Automatic calibrator of Raman detector and calibration method thereof

Abstract

The invention discloses an automatic calibrator of a Raman detector and a calibration method of the automatic calibrator, and aims to simplify the calibration process and improve the calibration efficiency and precision through reasonable structural design and automatic control. The automatic calibrator comprises a shell, a light source mechanism, a collimating mirror, a laser calibration mechanism and an optical probe, wherein the optical probe is aligned with other parts of the calibrator through an adapter. Besides, the calibrator is integrated with a USB interface and used for being connected with a microcomputer of a Raman detector, the laser calibration mechanism moves up and down through a motion control panel, it is ensured that an optical probe is aligned with a laser power meter and a Raman standard substance, and the automatic calibration process is completed.

Description

Technical Field

[0001] The invention belongs to the technical field of optical instrument calibration, and in particular relates to an automatic calibrator of a Raman detector and a calibration method thereof. Background Art

[0002] Raman detection is a spectral detection technology that is mainly based on the spectral analysis of the received optical signal to achieve precise judgment of the composition of the detected object. However, the Raman signal is very weak and requires a high degree of accuracy in the received spectrum, so high-precision spectrometers and other supporting equipment are required. Based on the high-precision requirements, after long-distance transportation or long-term use, the spectrometer in the Raman detector needs to be calibrated with a standard light source to ensure the accuracy of the diagnosis; and because the optical probe is a consumable and often needs to be replaced, and the loss and other characteristics of each optical probe are slightly different, the internal laser channel also needs to be aligned after each replacement of the optical probe.

[0003] The standard light source is based on the emission of rare gases with very stable characteristics. The wavelength stability of its spectrum is strong and it is rarely affected by the outside world. Therefore, it is often used as a reference standard for various calibration work. At the same time, for in-body detection, lasers with too high intensity will damage human tissues, and lasers with insufficient intensity will result in very weak detection signals. The loss of each optical fiber, optical probe, etc. is different, so the signal transmission channel (laser channel and spectrometer channel) also needs to be calibrated before use.

[0004] The Raman detector designed for gastric cancer detection needs to be detected by introducing a thin optical probe such as that provided by Chinese patent CN115420382A to meet the needs of in vivo detection. The existing calibration design generally builds a standard light source into the Raman detector, and connects it to the spectrometer through a customized one-to-two optical fiber bundle ("two" means that one of them is connected to the light source for calibration, and the other is connected to the optical probe for Raman detection), and controls it to work through software when calibration is required. As for the calibration of the output laser power of the optical probe, the commonly used method is to set up a power meter module, which is more accurate for the calibration of laser power, sufficient to ensure that the lasers emitted by different optical probes maintain a roughly consistent power, but because the received Raman optical signal also needs to be transmitted to the spectrometer through the optical probe, there is a lack of correction for the Raman signal, that is, it is impossible to calibrate the optical fiber connected to the spectrometer, which leads to poor signal consistency.

[0005] Since the light entrance of the spectrometer is a slit, the light needs to be introduced through a precisely arranged optical fiber bundle, so the optical fiber bundle and the slit need to be adjusted more precisely to ensure alignment, that is, at least the interface on the spectrometer end needs to be kept in an aligned fixed state, and cannot be an easily disassembled and movable structure. Therefore, the current common design is to assemble the light source inside the chassis of the Raman detector and introduce it into the spectrometer through a special optical fiber, that is, the whole is a set of devices fixed in the chassis for a long time. The standard light source and the bent optical fiber bundle both occupy a large internal space of the chassis, and also occupy part of the light entrance of the spectrometer, and the customized optical fiber also increases the cost.

[0006] Another design scheme is that one side of the optical fiber's spectrometer interface is fixed, while the other end is designed as a quick-release structure and placed in a position that is convenient for disassembly. An external calibration light source is connected to it through optical fiber, coupler, etc. for calibration. Similarly, although this scheme saves space in the chassis, it also occupies a part of the light input port of the spectrometer, and the cost of customizing components such as optical fiber and optical fiber coupler is also high.

[0007] In addition, the standard light source needs to be preheated for a certain period of time before it can emit light, and this preheating time is not stable, sometimes long and sometimes short. Unless the chassis is disassembled and the optical fiber bundle is removed, it is impossible to check whether the light source fixed inside the chassis has completed preheating (i.e., emitting a light beam). As a result, the calibration time is too long and the stability is poor. In actual use, calibration often fails due to incomplete preheating (the user cannot directly observe and confirm whether the preheating is completed), resulting in the need for repeated calibrations to succeed. Summary of the invention

[0008] In order to solve the above problems, the present invention discloses an automatic calibrator for a Raman detector and a calibration method thereof, aiming to simplify the calibration process and improve the calibration efficiency and accuracy through reasonable structural design and automatic control. The automatic calibrator includes a housing, a light source mechanism, a collimator, a laser calibration mechanism and an optical probe, and the optical probe is aligned with other parts of the calibrator through an adapter. In addition, the calibrator integrates a USB interface for connecting to a microcomputer of the Raman detector, and the laser calibration mechanism is moved up and down through a motion control board to ensure that the optical probe is aligned with the laser power meter and the Raman standard material, thereby completing the automatic calibration process.

[0009] To achieve the above object, the technical solution of the present invention is as follows:

[0010] An automatic calibrator for a Raman detector comprises a shell, wherein a light source mechanism, a collimator, a laser calibration mechanism, and an optical probe are arranged in the shell, a USB interface is arranged on one side of the shell, an adapter is arranged on the side of the shell away from the USB interface, the adapter is a cylindrical sleeve structure, openings are arranged at the front and rear ends of the adapter, the tail opening of the adapter is larger than the front opening, the optical probe is inserted into the adapter through the tail opening of the adapter, and the collimator and the adapter are on the same horizontal line.

[0011] As an improvement of the present invention, the light source mechanism includes a standard light source, a three-in-one optical fiber bundle, and a light source control panel. The three-in-one optical fiber bundle guides the light of the standard light source into a collimating lens and injects it into an adapter. The light source control panel is connected to the standard light source. A physical button and a circuit interface are provided on the shell, and the light source control panel is connected to the physical button and the circuit interface.

[0012] As an improvement of the present invention, the standard light source includes a Ne lamp, an Ar lamp, and a Xe lamp, and the standard light source contains a battery.

[0013] As an improvement of the present invention, the laser calibration mechanism includes a laser power meter, a Raman frequency shift standard material, a Raman intensity standard material, and a motion control board. The laser power meter, Raman frequency shift standard material, and Raman intensity standard material are arranged in parallel vertically. The motion control board is used to control the laser power meter, Raman frequency shift standard material, and Raman intensity standard material to move up and down. The motion control board is connected to the USB interface.

[0014] As an improvement of the present invention, the front inner portion of the adapter is a slope structure, so that the optical probe can be positioned at the exact center.

[0015] The present invention also provides a method for calibrating an automatic calibrator of a Raman detector, wherein the Raman detector is calibrated using the automatic calibrator, and the specific steps are as follows:

[0016] S1. Connect the USB interface to the microcomputer of the Raman detector, preheat the Raman detector, insert the optical probe into the adapter, start the light source mechanism, and start the automatic calibration procedure of the spectrometer of the Raman detector;

[0017] S2. The standard light sources are turned on in sequence, and the light beam is introduced into the spectrometer through the light probe. The spectrometer receives and identifies the incoming light once after waiting for a few seconds, and confirms whether it is the required standard light source by finding the number of peaks after normalization. After confirming the number of peaks, the spectrum is recorded, and the system goes through the steps of the corresponding light source. After the three light sources are recorded in sequence, the microcomputer automatically performs linear averaging to complete the spectrometer calibration process;

[0018] S3. After the spectrometer calibration is completed, all standard light sources are turned off, the motion control board is moved, the laser power meter is aligned with the adapter, the laser of the Raman detector is turned on, and the microcomputer automatically adjusts the laser output size according to the reading, so that the laser power output of the optical probe is stabilized near the preset value;

[0019] S4. Move the motion control board, align the Raman frequency shift standard material with the adapter, measure the spectrum of the standard material, and the microcomputer analyzes whether the spectrum conforms to the standard spectrum to confirm whether the calibrator is in normal working condition;

[0020] S5. Move the motion control board to align the Raman intensity standard material with the adapter. The microcomputer obtains the correction coefficient according to the Raman intensity calibration correction algorithm and the spectrum diagram and saves it.

[0021] As an improvement of the present invention, after the light source mechanism is started in step S1, a calibration procedure of a single standard light source can be performed by turning on a physical button alone.

[0022] As an improvement of the present invention, the Raman intensity calibration correction algorithm of step S5 has the following specific steps:

[0023] (1) Select several fiber optic probes of qualified quality as reference fibers, align them correctly with the Raman intensity standard material, measure the spectra, and take the average value of the light intensity of multiple spectra as the standard value, denoted as L 标 (λ), where L represents the light intensity and λ represents the wavelength;

[0024] (2) Align the optical fiber probe to be calibrated with the Raman intensity standard material to obtain the measured spectrum, and use the ratio of the measured spectrum to the spectrum standard value as the correction coefficient k n (λ), the specific calculation formula is:

[0025]

[0026] Where n is the fiber number, L n (λ) is the light intensity of the optical fiber numbered n at wavelength λ.

[0027] As an improvement of the present invention, when the Raman detector detects the sample to be tested, the light intensity at each wavelength λ is equal to the correction coefficient k corresponding to the wavelength. n (λ) to obtain the corrected spectrum. The specific calculation formula is:

[0028] L' n(修正) (λ) = k n (λ)L' n(测量) (λ);

[0029] Among them, L' n(修正)(λ) represents the final corrected light intensity at wavelength λ, L' n(测量) (λ) represents the light intensity directly measured at the wavelength.

[0030] The present invention also provides an application of an automatic calibrator for a Raman detector. The automatic calibrator can be applied to calibrate a gastric cancer Raman detector.

[0031] The beneficial effects of the present invention are:

[0032] (1) The automatic calibrator of the present invention uses a standard light source for calibration (i.e., a spectrometer calibration module) and no longer guides light through a dedicated optical fiber. Instead, the existing Raman optical fiber probe, which is a component already present in the Raman detector, is used to guide light into the spectrometer. By using the adapter of the present invention, the adapter can also perform calibration work, thereby reducing the cost of the Raman detector, simplifying its structure, and significantly reducing the size of the Raman detector.

[0033] (2) The present invention facilitates the insertion or removal of the optical probe by setting the adapter. Therefore, when the standard light source is preheated, it is possible to directly observe whether light is emitted (i.e., whether the preheating is completed) through the opening of the adapter. The adapter can be replaced and can be adapted to optical probes of various models and sizes. At the same time, the present invention eliminates the high-cost attenuation plate, coupler and other precision optical components that are originally set to prevent the spectrometer from being damaged by overexposure, and does not require the use of complex customized optical fibers, thereby reducing costs.

[0034] (3) The present invention separates the automatic calibrator from the Raman detector, which reduces the volume of the Raman detector, simplifies its structure, and can achieve one-to-many calibration (one calibrator can be used for multiple Raman detectors), reducing the overall cost. In addition, the present invention includes Raman intensity calibration, which improves the consistency of the detection signal through the correction algorithm, which helps to improve the accuracy of diagnosis.

[0035] (4) The present invention is provided with a USB interface. Since a microcomputer is already included inside the Raman detector, the present invention does not need to set up a separate computer. It only needs to be connected to the data interface on the Raman detector through a USB or other common universal data interface to realize the control of the entire calibration process and the analysis of spectral data. BRIEF DESCRIPTION OF THE DRAWINGS

[0036] Figure 1 This is a schematic diagram of the structure of an automated calibrator for a Raman detector, with some power supply wires omitted.

[0037] Figure 2 A schematic diagram of the structure of the adapter.

[0038] Figure 3 A cross-sectional view of the adapter.

[0039] Figure 4 The present invention is a flow chart of the calibration method of the automated calibrator of the Raman detector.

[0040] Figure 5 This is the spectrum of Xe light source.

[0041] List of Figure Symbols:

[0042] 1. Shell; 2. Collimator; 3. USB interface; 4. Adapter; 5. Standard light source; 6. Three-in-one optical fiber bundle; 7. Light source control panel; 8. Laser power meter; 9. Raman frequency shift standard material; 10. Raman intensity standard material; 11. Motion control board; 12. Ramp structure. DETAILED DESCRIPTION

[0043] The present invention will be further explained below in conjunction with the accompanying drawings and specific embodiments. It should be understood that the following specific embodiments are only used to illustrate the present invention and are not used to limit the scope of the present invention.

[0044] The standard substance described in the present invention is a special product for calibration provided by the Chinese Academy of Metrology.

[0045] Embodiment 1

[0046] An automatic calibrator for a Raman detector comprises a shell, wherein a light source mechanism, a collimator, a laser calibration mechanism, and an optical probe are arranged in the shell, a USB interface is arranged on one side of the shell, an adapter is arranged on the side of the shell away from the USB interface, the adapter is a cylindrical sleeve structure, openings are arranged at the front and rear ends of the adapter, the tail opening of the adapter is larger than the front opening, the optical probe is inserted into the adapter through the tail opening of the adapter, and the collimator and the adapter are on the same horizontal line.

[0047] The light source mechanism described in this embodiment includes a standard light source, a three-in-one optical fiber bundle, and a light source control panel. The three-in-one optical fiber bundle guides the light of the standard light source into a collimating lens and then injects it into an adapter. The light source control panel is connected to the standard light source. Physical buttons and circuit interfaces are provided on the shell, and the light source control panel is connected to the physical buttons and circuit interfaces.

[0048] The laser calibration mechanism described in this embodiment includes a laser power meter, a Raman frequency shift standard material, a Raman intensity standard material, and a motion control board. The laser power meter, Raman frequency shift standard material, and Raman intensity standard material are arranged in parallel vertically. The motion control board is used to control the laser power meter, Raman frequency shift standard material, and Raman intensity standard material to move up and down. The motion control board is connected to the USB interface.

[0049] Embodiment 2

[0050] On the basis of the first embodiment, the standard power source includes Ne lamp, Ar lamp and Xe lamp. The standard light source contains a battery, which only needs to be charged before use, and is not restricted by power supply wires.

[0051] Embodiment 3

[0052] Based on the first embodiment, the front inner side of the adapter is a slope structure, so that the optical probe can be positioned at the exact center. The adapter can be made of metal or plastic. When in use, the optical probe can be directly inserted from the tail and extended to the bottom. It can be fixed on the calibrator by tilting the installation method, and gravity is used to ensure that it will not fall off naturally. At the same time, since the front opening is designed according to the size of the optical probe, it can meet the needs of fixing the probe and accurately aligning the optical path.

[0053] Embodiment 4

[0054] The spectrometer of the Raman detector is calibrated using any one of the automated calibrators in Examples 1 to 3, and the specific steps are as follows:

[0055] S1. Connect the USB interface to the microcomputer of the Raman detector, preheat the Raman detector, insert the optical probe into the adapter, start the light source mechanism, and start the automatic calibration procedure of the spectrometer of the Raman detector;

[0056] S2. The standard light sources are turned on in sequence, and the light beam is introduced into the spectrometer through the light probe. The spectrometer receives and identifies the incoming light once after waiting for a few seconds, and confirms whether it is the required standard light source by finding the number of peaks after normalization. After confirming the number of peaks, the spectrum is recorded, and the system goes through the steps of the corresponding light source. After the three light sources are recorded in sequence, the microcomputer automatically performs linear averaging to complete the spectrometer calibration process;

[0057] S3. After the spectrometer calibration is completed, all standard light sources are turned off, the motion control board is moved, the laser power meter is aligned with the adapter, the laser of the Raman detector is turned on, and the microcomputer automatically adjusts the laser output size according to the reading, so that the laser power output of the optical probe is stabilized near the preset value;

[0058] S4. Move the motion control board, align the Raman frequency shift standard material with the adapter, measure the spectrum of the standard material, and the microcomputer analyzes whether the spectrum conforms to the standard spectrum to confirm whether the calibrator is in normal working condition;

[0059] S5. Move the motion control board to align the Raman intensity standard material with the adapter, and the microcomputer obtains the correction coefficient according to the Raman intensity calibration correction algorithm and the spectrum, and saves it;

[0060] Correction factor k n The specific calculation formula of (λ) is:

[0061]

[0062] Where: L 标 (λ) is the standard value, n is the fiber number, L n (λ) is the light intensity of the optical fiber numbered n at wavelength L. 标 (λ) By selecting several fiber optic probes of qualified quality as reference fibers, aligning them correctly with the Raman intensity standard material, measuring and obtaining spectra, the average light intensity of multiple spectra is taken as the standard value, where L represents the light intensity and λ represents the wavelength.

[0063] S6. When the Raman detector detects the sample to be tested, the light intensity at each wavelength λ is the correction factor k corresponding to the wavelength. n (λ) to obtain the corrected spectrum. The specific calculation formula is:

[0064] L' n(修正) (λ) = k n (λ)L' n(测量) (λ)

[0065] Among them, L' n(修正) (λ) represents the final corrected light intensity at wavelength λ, L' n(测量) (λ) represents the light intensity directly measured at the wavelength.

[0066] Embodiment 5

[0067] Based on the fourth embodiment, the spectrometer can also perform a manual calibration procedure of a single standard light source by turning on a physical button alone.

[0068] The detection principle of the calibration method of the automatic calibrator of a Raman detector of the present invention is:

[0069] (1) Spectrometer calibration principle:

[0070] The mechanism of standard light source calibration is to take the standard light source Xe lamp as an example, and its spectrum is as shown in the attached figure. Figure 5 As shown, the gastric cancer Raman detector calibrated by the present invention uses a 785nm laser, so the band near 785nm is intercepted. The arrows in the figure indicate several very obvious light intensity peaks with large signal-to-noise ratio. The spectra of the other two standard light sources, Ne and Ar lamps, are also similar, except that the wavelengths corresponding to the multiple peaks in the spectra of different light sources are different. The purpose of using multiple light sources is to subdivide the area as much as possible and ensure accuracy as much as possible. Since rare gases are very stable, the wavelengths corresponding to the above-mentioned light intensity peaks are also very fixed and are almost unaffected by environmental changes. Therefore, they can be used as a reference standard to calibrate the spectrometer (only the wavelength accuracy is required when calibrating the spectrometer, and it has nothing to do with the light intensity).

[0071] The light emitted by the standard light source enters the light entrance of the spectrometer through optical fibers and other devices. The spectrometer confirms several accurate wavelength positions by receiving the peak light intensity of the light. The three light sources provide a total of more than ten standard lights of different wavelengths, which are finally linearly divided between adjacent peaks to achieve full-band wavelength calibration to maximize the accuracy of the calibration.

[0072] Spectrometer calibration can be divided into automatic calibration mode and manual calibration mode, where:

[0073] The automatic calibration mode of the spectrometer is to complete the preheating work first, then insert the optical probe into the adapter, press the calibration button, the light source is turned on in turn, the light beam is introduced into the spectrometer through the optical probe, and the spectrometer receives and recognizes the incoming light once after waiting for a few seconds. It confirms whether it is the required standard light source (such as the attached Figure 2 The Xe lamp shown should find 8 peaks), record the spectrum after confirming the number of peaks, and in the system, complete the collection of the three light sources in turn through the steps of the corresponding light source, and then automatically perform linear averaging through the computer to complete the spectrometer calibration process. Linear averaging refers to directly and evenly distributing the points that lack precise information between two adjacent feature points according to the coordinate interval. For example, in a certain calibration process, the light (peak position) provided by the standard light source can accurately confirm points such as 860nm and 875nm, and the middle part is distributed by an average "cutting" method. Obviously, although it is impossible to directly obtain the feature points of all wavelengths, the more standard feature points are obtained, the more accurate the linear averaging will be. Therefore, the present invention receives the input light of three light sources, obtains nearly 20 feature points, and then performs linear averaging to minimize the interval of each linear averaging and obtain the most accurate calibration effect.

[0074] The logic of manual mode is relatively simple, that is, manually turn on the switch of any light source as needed, and the light beam is emitted from the adapter for calibration. It is mainly used for individual debugging or defect detection and leakage repair.

[0075] The automatic calibration mode of the spectrometer is to complete the preheating work first, then insert the optical probe into the adapter, press the calibration button, the light source is turned on in turn, the light beam is introduced into the spectrometer through the optical probe, and the spectrometer receives and recognizes the incoming light once after waiting for a few seconds. It confirms whether it is the required standard light source (such as the attached Figure 2 The Xe lamp shown in the figure should find 8 peaks), record the spectrum after confirming the number of peaks, and go through the steps of the corresponding light source in the system, complete the collection of the three light sources in turn, and then automatically perform linear averaging through the computer to complete the spectrometer calibration process.

[0076] Linear averaging refers to directly averaging the points between two adjacent feature points that lack precise information according to the coordinate interval. For example, in a calibration process, the light (peak position) provided by a standard light source can accurately confirm points such as 860nm and 875nm, and the middle part is distributed by an average "cutting" method. Obviously, although it is impossible to directly obtain feature points of all wavelengths, the more standard feature points are obtained, the more accurate the linear averaging will be. Therefore, the present invention receives input light from three light sources, obtains nearly 20 feature points, and then performs linear averaging to minimize the interval of each linear averaging and obtain the most accurate calibration effect.

[0077] The logic of manual mode is relatively simple, that is, manually turn on the switch of any light source as needed, and the light beam is emitted from the adapter for calibration. It is mainly used for individual debugging or defect detection and leakage repair.

[0078] (2) Raman intensity calibration principle:

[0079] After completing the spectrometer calibration steps, the laser calibration procedure is entered. The motor moves the displacement module, and the output of the laser is calibrated using a laser power meter, Raman frequency shift standard material, and Raman intensity standard material, and the output of the spectrum detection is preliminarily standardized and corrected.

[0080] Laser power meter: Real-time measurement of the laser output power of the optical probe. The computer automatically adjusts through data feedback to increase or decrease the laser output and finally reaches the preset range (such as 75-85mW). (Note: The laser is located inside the Raman detector. The above output adjustment steps are actually the microcomputer inside the Raman detector controlling the components inside the Raman detector to make adjustments. They do not belong to the components of the present invention. The present invention only plays the role of feeding back the power meter data)

[0081] Raman frequency shift standard material: After the laser power calibration is completed, a spectrum measurement of the standard material is performed. Its Raman spectrum is basically fixed. Using AI recognition, the computer can automatically confirm whether the current test result of the detector is accurate to confirm whether it is in normal working condition.

[0082] Raman intensity standard material: Since there are individual differences in the transmission loss of each optical probe, even if the outgoing laser intensity has been calibrated, the absolute intensity consistency of the optical signal received by the spectrometer is still low, which has a certain impact on the subsequent analysis of the test results using AI. Therefore, the present invention introduces a Raman intensity standard material to calibrate the intensity of the Raman detection signal. Since the proportion of Raman light in the reflected light is extremely low, the Raman signal is very weak, and the Raman scattering intensity of each wavelength is relatively obvious and the difference is not large. The whole presents a slightly undulating and soothing arc. Compared with other common substances such as Raman frequency shift standard materials and samples, there will be no situation where there are strong Raman peaks only at a few wavelengths and almost no visible signal feedback at most wavelengths. Therefore, through intensity calibration, the correction coefficient is obtained using the Raman intensity standard material, which is used to gain or weaken the obtained detection signal when detecting the sample, and to correct the spectrum of the sample detection. The corrected spectrum can improve the consistency of the analysis results and facilitate subsequent analysis and diagnosis.

[0083] It should be noted that the drawings only illustrate the technical ideas of the present invention, and the protection scope of the present invention cannot be limited by this shape. For ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principles of the present invention. These improvements and modifications all fall within the protection scope of the claims of the present invention.

Claims

1. An automatic calibrator for a Raman detector, comprising a housing, wherein a light source mechanism, a collimator, a laser calibration mechanism, and an optical probe are arranged in the housing, characterized in that: A USB interface is provided on one side of the shell, and an adapter is provided on the side of the shell away from the USB interface. The adapter is a cylindrical sleeve structure, and openings are provided at the front and rear ends of the adapter. The tail opening of the adapter is larger than the front opening. The optical probe is inserted into the adapter through the tail opening of the adapter, and the collimator and the adapter are on the same horizontal line.

2. The automatic calibrator of the Raman detector and the calibration method thereof according to claim 1, characterized in that: The light source mechanism includes a standard light source, a three-in-one optical fiber bundle, and a light source control panel. The three-in-one optical fiber bundle guides the light of the standard light source into a collimating lens and then injects it into an adapter. The light source control panel is connected to the standard light source. Physical buttons and circuit interfaces are provided on the housing, and the light source control panel is connected to the physical buttons and circuit interfaces.

3. The automatic calibrator of Raman detector and the calibration method thereof according to claim 2, characterized in that: The standard light source includes a Ne lamp, an Ar lamp, and a Xe lamp, and the standard light source contains a battery.

4. The automatic calibrator for Raman detector according to claim 2, characterized in that: The laser calibration mechanism includes a laser power meter, a Raman frequency shift standard material, a Raman intensity standard material, and a motion control board. The laser power meter, Raman frequency shift standard material, and Raman intensity standard material are arranged in parallel and longitudinally. The motion control board is used to control the laser power meter, Raman frequency shift standard material, and Raman intensity standard material to move up and down. The motion control board is connected to the USB interface.

5. The automatic calibrator of the Raman detector according to any one of claims 1 to 4, characterized in that: The front inner portion of the adapter is a slope structure, so that the optical probe can be positioned at the exact center.

6. A method for calibrating an automated calibrator of a Raman detector, characterized in that: The Raman detector is calibrated using the automated calibrator described in claim 4, and the specific steps are as follows: S1. Connect the USB interface to the microcomputer of the Raman detector, preheat the Raman detector, insert the optical probe into the adapter, start the light source mechanism, and start the automatic calibration procedure of the spectrometer of the Raman detector; S2. The standard light sources are turned on in sequence, and the light beam is introduced into the spectrometer through the light probe. The spectrometer receives and identifies the incoming light once after waiting for a few seconds, and confirms whether it is the required standard light source by finding the number of peaks after normalization. After confirming the number of peaks, the spectrum is recorded, and the system goes through the steps of the corresponding light source. After the three light sources are recorded in sequence, the microcomputer automatically performs linear averaging to complete the spectrometer calibration process; S3. After the spectrometer calibration is completed, all standard light sources are turned off, the motion control board is moved, the laser power meter is aligned with the adapter, the laser of the Raman detector is turned on, and the microcomputer automatically adjusts the laser output size according to the reading, so that the laser power output of the optical probe is stabilized near the preset value; S4. Move the motion control board, align the Raman frequency shift standard material with the adapter, measure the spectrum of the standard material, and the microcomputer analyzes whether the spectrum conforms to the standard spectrum to confirm whether the calibrator is in normal working condition; S5. Move the motion control board to align the Raman intensity standard material with the adapter. The microcomputer obtains the correction coefficient according to the Raman intensity calibration correction algorithm and the spectrum diagram and saves it.

7. The calibration method of the automated calibrator of the Raman detector according to claim 6, characterized in that: After the light source mechanism is started in step S1, a calibration procedure of a single standard light source can be performed by turning on a physical button alone.

8. The calibration method of the automated calibrator of the Raman detector according to claim 6, characterized in that: The specific steps of the Raman intensity calibration correction algorithm in step S5 are as follows: (1) Select several fiber optic probes of qualified quality as reference fibers, align them correctly with the Raman intensity standard material, measure the spectra, and take the average value of the light intensity of multiple spectra as the standard value, denoted as L 标 (λ), where L represents the light intensity and λ represents the wavelength; (2) Align the optical fiber probe to be calibrated with the Raman intensity standard material to obtain the spectrum to be measured, and use the ratio of the spectrum to be measured to the standard value as the correction coefficient k n (λ), the specific calculation formula is: Where n is the fiber number, L n (λ) is the light intensity of the optical fiber numbered n at wavelength λ.

9. The calibration method of the automated calibrator of the Raman detector according to claim 8, characterized in that: When the Raman detector detects the sample to be tested, the light intensity at each wavelength λ is equal to the correction coefficient k corresponding to the wavelength. n (λ) to obtain the corrected spectrum. The specific calculation formula is: The n(修正) (λ)=k n (λ)L' n(测量) (l); Among them, L' n(修正) (λ) represents the final corrected light intensity at wavelength λ, L' n(测量) (λ) represents the light intensity directly measured at the wavelength.

10. An application of an automatic calibrator for a Raman detector according to any one of claims 1 to 5, characterized in that: Application of the automatic calibrator in a gastric cancer Raman detector.

Images