Herbal cream with efficient bacteriostasis, preparation method and application

Abstract

The invention provides herbal emulsifiable paste with efficient bacteriostasis as well as a preparation method and application of the herbal emulsifiable paste. The herbal cream is prepared from the following components in parts by mass: 10 to 13 parts of geranium extract, 15 to 17 parts of thyme extract, 1 to 3 parts of peppermint essential oil, 3 to 6 parts of honeysuckle flower extract, 1 to 4 parts of chrysanthemum flower extract, 2 to 3 parts of glycerol, 0.2 to 0.5 part of sodium carboxymethyl cellulose, 0.2 to 0.6 part of methyl p-hydroxybenzoate, 2 to 4 parts of octadecanoic acid, 2 to 3 parts of emulsifier, 1.2 to 1.6 parts of squalane and 50 to 60 parts of water. According to the herbal cream disclosed by the invention, various components are compounded and synergistically play a role, and the prepared herbal cream is mild, non-irritant and good in antibacterial effect.

Description

Technical Field

[0001] The invention belongs to the technical field of herbal creams, and in particular relates to a herbal cream with high-efficiency antibacterial properties, a preparation method and an application thereof. Background Art

[0002] Skin antibacterial creams are mainly used to treat and prevent skin infections and inflammations caused by bacteria, fungi or other microorganisms. Their significance is reflected in the following aspects: Antibacterial and bactericidal: Skin antibacterial creams containing antibacterial and antifungal ingredients can act directly on the skin surface, effectively inhibiting or killing common skin pathogens, such as Staphylococcus, Streptococcus, Epidermophyton, Trichophyton, Microsporum, etc., thereby preventing or controlling the further development of infection. Relieve symptoms: For skin diseases such as eczema, urticaria, dermatitis, and ringworm (such as tinea manuum, tinea pedis, tinea cruris, and tinea corporis), antibacterial creams can help relieve symptoms such as itching, redness, swelling, pain, dryness, and desquamation, and improve the patient's quality of life. Promote healing: The ingredients in some antibacterial creams can also promote the repair and healing of local skin tissues, such as by improving blood circulation, moisturizing the skin, and accelerating the regeneration of damaged skin and restoring it to normal. Preventing the spread of infection: In cases of skin trauma, abrasions, or surgical incisions, using antibacterial skin creams can help prevent wound infection, especially in high-risk environments or among people with weakened immune systems. Daily care: For skin areas prone to infection or recurrent infection, antibacterial creams can be used as part of personal hygiene products for daily skin care to prevent skin problems caused by contact with microorganisms in the environment.

[0003] Antibacterial skin cream plays an important role in the prevention and treatment of skin diseases. It is one of the common medicines for clinical treatment in dermatology and household medicines. However, the antibacterial effect of the antibacterial creams on the market is not ideal, the effect of use is not significant, and adverse reactions are prone to occur. Summary of the invention

[0004] The purpose of the invention is to provide a herbal cream with high-efficiency antibacterial effect, a preparation method and application thereof.

[0005] In order to achieve the above-mentioned purpose, the present invention provides the following technical scheme: a herbal cream with high-efficiency antibacterial effect, comprising the following components in parts by weight: 10-13 parts of geranium extract, 15-17 parts of thyme extract, 1-3 parts of peppermint essential oil, 3-6 parts of honeysuckle extract, 1-4 parts of chrysanthemum extract, 2-3 parts of glycerol, 0.2-0.5 parts of sodium carboxymethyl cellulose, 0.2-0.6 parts of methyl parahydroxybenzoate, 2-4 parts of octadecanoic acid, 2-3 parts of emulsifier, 1.2-1.6 parts of squalane and 50-60 parts of water.

[0006] Furthermore, the preparation method of the geranium extract is:

[0007] (1) adding 12-15% by mass of geranium flowers to YPD medium and sterilizing by high pressure steam to obtain geranium medium;

[0008] (2) The yeast was inoculated into YPD medium and cultured at 32-35°C and 160-170 rpm to obtain a seed solution, wherein the yeast content in the seed solution was 3×10 8 -6×10 8 CFU / mL;

[0009] (3) inoculating the seed bacterial liquid into the geranium culture medium at a volume ratio of 1:7-9, culturing at 30-32° C. and 100-120 rpm for 22-25 hours to obtain a fermentation product; subjecting the fermentation product to high-pressure homogenization to obtain a homogenate, centrifuging, and taking a supernatant;

[0010] (4) adding 0.2-0.3% by mass of complex enzyme to the supernatant, performing enzymolysis at 45-48° C. for 3-4 h, inactivating the enzyme at 90° C. for 30 min, and spray drying to obtain a geranium extract.

[0011] Further, high pressure homogenization treatment: pressure 800-820Bar, homogenization 3 times.

[0012] Further, high pressure steam sterilization was performed at 121°C for 30 min.

[0013] Furthermore, the complex enzyme is composed of alkaline protease, papain and flavor protease in a mass ratio of 0.5-0.8:1:1.6-1.9.

[0014] Furthermore, the enzyme activity of alkaline protease is 100,000 U / g; the enzyme activity of papain is 50,000 U / g; and the enzyme activity of flavor protease is 200,000 U / g.

[0015] Furthermore, the preparation method of the thyme extract is:

[0016] (1) grinding thyme through a 100-mesh sieve to obtain thyme powder, mixing 70-80 parts by weight of the thyme powder with 200-300 parts by weight of purified water, and sterilizing to obtain a mixed liquid;

[0017] (2) selecting Lactobacillus brevis, Lactobacillus plantarum, Lactobacillus acidophilus, and Bifidobacterium lactis as mixed bacterial agents; adding 1 part by mass of the mixed bacterial agent and the mixed liquid of step (1) to a fermentation tank, adjusting the pH to 6.3, and performing anaerobically fermentation at 36° C. for 3-5 days. After the fermentation is completed, sterilization is performed to obtain a fermentation system;

[0018] (3) The fermentation system was centrifuged at 8000-9000 rpm for 10-15 min, filtered through a 0.22 μm membrane, the supernatant was taken, and spray-dried to obtain a thyme extract.

[0019] Furthermore, the mass ratio of Lactobacillus brevis, Lactobacillus plantarum, Lactobacillus acidophilus and Bifidobacterium lactis is 2.5-3:0.8-1.2:0.3-0.6:0.7-1.

[0020] Furthermore, Lactobacillus brevis, with an active bacterial count of 10 billion CFU / g, and a preservation number of SHBCCD14346; Lactobacillus plantarum, with an active bacterial count of 10 billion CFU / g, and a preservation number of CCTCCAB2010210; Lactobacillus acidophilus, with an active bacterial count of 20 billion CFU / g, and a preservation number of CCTCCAB2010208; and Bifidobacterium lactis, with an active bacterial count of 10 billion CFU / g, and a preservation number of SHBCCD24675.

[0021] Furthermore, the emulsifier is selected from one or more of Tween 60, Tween 80 and Span 60.

[0022] The present invention provides a method for preparing a herbal cream with high antibacterial effect, comprising the following steps:

[0023] (1) firstly mix geranium extract, chrysanthemum extract, peppermint essential oil, honeysuckle extract, thyme extract and water, stir evenly, heat to 80-85° C. and keep warm for 40-50 min to obtain phase A component;

[0024] (2) Mix the remaining components, heat and stir evenly, raise the temperature to 70-75°C and keep warm for 20-30 minutes to obtain phase B component;

[0025] (3) Add the phase A component to the phase B component and perform homogenization at a homogenization speed of 11-12 krpm for 6-8 min. After the homogenization is completed, stir to obtain a herbal cream with high antibacterial efficiency.

[0026] The invention also provides the application of the herbal cream with high-efficiency bacteriostasis.

[0027] Compared with the prior art, the advantages and beneficial effects of the present invention are as follows: the present invention uses a plurality of components to compound and work synergistically, and the prepared herbal cream is mild and non-irritating, and has a good antibacterial effect. The present invention uses geranium extract and thyme extract prepared by a specific method and supplemented with other various natural extract ingredients, which have a synergistic effect and enhance the antibacterial effect of the antibacterial cream. The herbal cream of the present invention uses pure natural extracts as the main antibacterial ingredients, has a good antibacterial effect, has no side effects, is highly safe to use, and has good economic value and application prospects. DETAILED DESCRIPTION

[0028] The technical solutions in the embodiments of the present invention are described clearly and completely below. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0029] Example 1

[0030] This embodiment provides a herbal cream with high antibacterial effect, comprising the following components in parts by weight: 12 parts of geranium extract, 16 parts of thyme extract, 2 parts of peppermint essential oil, 4 parts of honeysuckle extract, 3 parts of chrysanthemum extract, 2.6 parts of glycerol, 0.4 parts of sodium carboxymethyl cellulose, 0.3 parts of methyl parahydroxybenzoate, 3 parts of octadecanoic acid, 2.3 parts of emulsifier, 1.5 parts of squalane and 55 parts of water.

[0031] The preparation method of the geranium extract is:

[0032] (1) adding 13% by mass of geranium flowers to YPD medium and sterilizing by high pressure steam to obtain geranium medium;

[0033] (2) The yeast was inoculated into YPD medium and cultured at 33°C and 165 rpm to obtain a seed solution, wherein the yeast content in the seed solution was 5×10 8 CFU / mL;

[0034] (3) inoculating the seed bacterial liquid into the geranium culture medium at a volume ratio of 1:8, culturing at 31° C. and 110 rpm for 24 hours to obtain a fermentation product; subjecting the fermentation product to high-pressure homogenization to obtain a homogenate, centrifuging, and taking a supernatant;

[0035] (4) adding 0.3% by mass of complex enzyme to the supernatant, performing enzymolysis at 46° C. for 3.5 h, inactivating the enzyme at 90° C. for 30 min, and spray drying to obtain a geranium extract.

[0036] High-pressure homogenization: pressure 810Bar, homogenization 3 times.

[0037] Sterilize with high pressure steam at 121℃ for 30min.

[0038] The complex enzyme is composed of alkaline protease, papain and flavor protease in a mass ratio of 0.7:1:1.7.

[0039] The enzyme activity of alkaline protease is 100,000 U / g; the enzyme activity of papain is 50,000 U / g; the enzyme activity of flavor protease is 200,000 U / g.

[0040] The preparation method of the thyme extract is:

[0041] (1) grinding thyme through a 100-mesh sieve to obtain thyme powder, mixing 75 parts by mass of the thyme powder and 230 parts by mass of purified water, and sterilizing to obtain a mixed liquid;

[0042] (2) selecting Lactobacillus brevis, Lactobacillus plantarum, Lactobacillus acidophilus, and Bifidobacterium lactis as mixed bacterial agents; adding 1 part by mass of the mixed bacterial agent and the mixed liquid of step (1) to a fermentation tank, adjusting the pH to 6.3, and performing anaerobically fermentation at 36° C. for 4 days. After the fermentation is completed, sterilization is performed to obtain a fermentation system;

[0043] (3) The fermentation system was centrifuged at 8500 rpm for 12 min, filtered through a 0.22 μm membrane, the supernatant was taken, and spray-dried to obtain a thyme extract.

[0044] The mass ratio of the Lactobacillus brevis, Lactobacillus plantarum, Lactobacillus acidophilus and Bifidobacterium lactis is 2.7:0.9:0.4:1.

[0045] Lactobacillus brevis, bacterial activity 10 billion CFU / g. Collection number: SHBCCD14346. Deposited in Shanghai Microbiological Collection Center.

[0046] Lactobacillus plantarum, bacterial activity 10 billion CFU / g. Collection number: CCTCCAB2010210. Deposited in China Center for Type Culture Collection.

[0047] Lactobacillus acidophilus, bacterial activity 20 billion CFU / g. Collection number: CCTCCAB2010208. Deposited in China Center for Type Culture Collection.

[0048] Bifidobacterium lactis, with an activity of 10 billion CFU / g. Collection number: SHBCCD24675. Deposited in Shanghai Microbiological Collection Center.

[0049] The emulsifier is Tween 60.

[0050] The preparation method of the herbal cream with high antibacterial effect comprises the following steps:

[0051] (1) firstly mix geranium extract, chrysanthemum extract, peppermint essential oil, honeysuckle extract, thyme extract and water, stir evenly, heat to 82° C. and keep warm for 45 minutes to obtain phase A component;

[0052] (2) Mix the remaining components, heat and stir evenly, raise the temperature to 72°C and keep warm for 25 minutes to obtain phase B component;

[0053] (3) Add the phase A component to the phase B component and perform homogenization at a homogenization speed of 12 krpm for 7 min. After the homogenization is completed, stir to obtain a herbal cream with high antibacterial efficiency.

[0054] Example 2

[0055] This embodiment provides a herbal cream with high antibacterial effect, comprising the following components in parts by weight: 10 parts of geranium extract, 17 parts of thyme extract, 1 part of peppermint essential oil, 6 parts of honeysuckle extract, 1 part of chrysanthemum extract, 3 parts of glycerol, 0.2 parts of sodium carboxymethyl cellulose, 0.6 parts of methyl parahydroxybenzoate, 2 parts of octadecanoic acid, 3 parts of emulsifier, 1.2 parts of squalane and 60 parts of water.

[0056] The preparation method of the geranium extract is:

[0057] (1) adding 12% by mass of geranium flowers to YPD medium and sterilizing by high pressure steam to obtain geranium medium;

[0058] (2) The yeast was inoculated into YPD medium and cultured at 32°C and 170 rpm to obtain a seed culture solution, wherein the yeast content in the seed culture solution was 3×10 8 CFU / mL;

[0059] (3) inoculating the seed bacterial liquid into the geranium culture medium at a volume ratio of 1:7, culturing at 32° C. and 100 rpm for 22 hours to obtain a fermentation product; subjecting the fermentation product to high-pressure homogenization to obtain a homogenate, centrifuging, and taking a supernatant;

[0060] (4) adding 0.3% by mass of complex enzyme to the supernatant, performing enzymolysis at 48° C. for 4 h, inactivating the enzyme at 90° C. for 30 min, and spray drying to obtain a geranium extract.

[0061] High-pressure homogenization: pressure 820Bar, homogenization 3 times.

[0062] Sterilize with high pressure steam at 121℃ for 30min.

[0063] The complex enzyme is composed of alkaline protease, papain and flavor protease in a mass ratio of 0.8:1:1.6.

[0064] The enzyme activity of alkaline protease is 100,000 U / g; the enzyme activity of papain is 50,000 U / g; the enzyme activity of flavor protease is 200,000 U / g.

[0065] The preparation method of the thyme extract is:

[0066] (1) grinding thyme through a 100-mesh sieve to obtain thyme powder, mixing 80 parts by weight of thyme powder and 200 parts by weight of purified water, and sterilizing to obtain a mixed liquid;

[0067] (2) selecting Lactobacillus brevis, Lactobacillus plantarum, Lactobacillus acidophilus, and Bifidobacterium lactis as mixed bacterial agents; adding 1 part by mass of the mixed bacterial agent and the mixed liquid of step (1) to a fermentation tank, adjusting the pH to 6.3, and performing anaerobically fermentation at 36° C. for 5 days. After the fermentation is completed, sterilization is performed to obtain a fermentation system;

[0068] (3) The fermentation system was centrifuged at 8000 rpm for 15 min, filtered through a 0.22 μm membrane, the supernatant was taken, and spray-dried to obtain a thyme extract.

[0069] The mass ratio of the Lactobacillus brevis, Lactobacillus plantarum, Lactobacillus acidophilus and Bifidobacterium lactis is 2.5:0.8:0.6:1.

[0070] Lactobacillus brevis, bacterial activity 10 billion CFU / g. Collection number: SHBCCD14346. Deposited in Shanghai Microbiological Collection Center.

[0071] Lactobacillus plantarum, bacterial activity 10 billion CFU / g. Collection number: CCTCCAB2010210. Deposited in China Center for Type Culture Collection.

[0072] Lactobacillus acidophilus, bacterial activity 20 billion CFU / g. Collection number: CCTCCAB2010208. Deposited in China Center for Type Culture Collection.

[0073] Bifidobacterium lactis, with an activity of 10 billion CFU / g. Collection number: SHBCCD24675. Deposited in Shanghai Microbiological Collection Center.

[0074] The emulsifier is selected from one or more of Tween 60, Tween 80 and Span 60.

[0075] The preparation method of the herbal cream with high antibacterial effect comprises the following steps:

[0076] (1) firstly mix geranium extract, chrysanthemum extract, peppermint essential oil, honeysuckle extract, thyme extract and water, stir evenly, heat to 85° C. and keep warm for 50 min to obtain phase A component;

[0077] (2) Mix the remaining components, heat and stir evenly, raise the temperature to 75°C and keep warm for 30 minutes to obtain phase B component;

[0078] (3) Add the phase A component to the phase B component and perform a homogenization operation at a homogenization speed of 11 krpm for 6 min. After the homogenization is completed, stir to obtain a herbal cream with high antibacterial efficiency.

[0079] Comparative Example 1

[0080] The difference between this comparative example and Example 1 is: raw material composition.

[0081] A herbal cream with high antibacterial effect comprises the following components in parts by weight: 21 parts of geranium extract, 16 parts of thyme extract, 2.6 parts of glycerol, 0.4 parts of sodium carboxymethylcellulose, 0.3 parts of methyl parahydroxybenzoate, 3 parts of octadecanoic acid, 2.3 parts of emulsifier, 1.5 parts of squalane and 55 parts of water.

[0082] The preparation method of the geranium extract is:

[0083] (1) adding 13% by mass of geranium flowers to YPD medium and sterilizing by high pressure steam to obtain geranium medium;

[0084] (2) The yeast was inoculated into YPD medium and cultured at 33°C and 165 rpm to obtain a seed solution, wherein the yeast content in the seed solution was 5×10 8 CFU / mL;

[0085] (3) inoculating the seed bacterial liquid into the geranium culture medium at a volume ratio of 1:8, culturing at 31° C. and 110 rpm for 24 hours to obtain a fermentation product; subjecting the fermentation product to high-pressure homogenization to obtain a homogenate, centrifuging, and taking a supernatant;

[0086] (4) adding 0.3% by mass of complex enzyme to the supernatant, performing enzymolysis at 46° C. for 3.5 h, inactivating the enzyme at 90° C. for 30 min, and spray drying to obtain a geranium extract.

[0087] High-pressure homogenization: pressure 810Bar, homogenization 3 times.

[0088] Sterilize with high pressure steam at 121℃ for 30min.

[0089] The complex enzyme is composed of alkaline protease, papain and flavor protease in a mass ratio of 0.7:1:1.7.

[0090] The enzyme activity of alkaline protease is 100,000 U / g; the enzyme activity of papain is 50,000 U / g; the enzyme activity of flavor protease is 200,000 U / g.

[0091] The preparation method of the thyme extract is:

[0092] (1) grinding thyme through a 100-mesh sieve to obtain thyme powder, mixing 75 parts by mass of the thyme powder and 230 parts by mass of purified water, and sterilizing to obtain a mixed liquid;

[0093] (2) selecting Lactobacillus brevis, Lactobacillus plantarum, Lactobacillus acidophilus, and Bifidobacterium lactis as mixed bacterial agents; adding 1 part by mass of the mixed bacterial agent and the mixed liquid of step (1) to a fermentation tank, adjusting the pH to 6.3, and performing anaerobically fermentation at 36° C. for 4 days. After the fermentation is completed, sterilization is performed to obtain a fermentation system;

[0094] (3) The fermentation system was centrifuged at 8500 rpm for 12 min, filtered through a 0.22 μm membrane, the supernatant was taken, and spray-dried to obtain a thyme extract.

[0095] The mass ratio of the Lactobacillus brevis, Lactobacillus plantarum, Lactobacillus acidophilus and Bifidobacterium lactis is 2.7:0.9:0.4:1.

[0096] Lactobacillus brevis, bacterial activity 10 billion CFU / g. Collection number: SHBCCD14346. Deposited in Shanghai Microbiological Collection Center.

[0097] Lactobacillus plantarum, bacterial activity 10 billion CFU / g. Collection number: CCTCCAB2010210. Deposited in China Center for Type Culture Collection.

[0098] Lactobacillus acidophilus, bacterial activity 20 billion CFU / g. Collection number: CCTCCAB2010208. Deposited in China Center for Type Culture Collection.

[0099] Bifidobacterium lactis, with an activity of 10 billion CFU / g. Collection number: SHBCCD24675. Deposited in Shanghai Microbiological Collection Center.

[0100] The emulsifier is Tween 60.

[0101] The preparation method of the herbal cream with high antibacterial effect comprises the following steps:

[0102] (1) firstly mix the geranium extract, the thyme extract and water, stir evenly, and then heat to 82° C. and keep the temperature for 45 min to obtain a phase A component;

[0103] (2) Mix the remaining components, heat and stir evenly, raise the temperature to 72°C and keep warm for 25 minutes to obtain phase B component;

[0104] (3) Add the phase A component to the phase B component and perform homogenization at a homogenization speed of 12 krpm for 7 min. After the homogenization is completed, stir to obtain a herbal cream with high antibacterial efficiency.

[0105] Comparative Example 2

[0106] The difference between this comparative example and Example 1 is that the composition ratio is different.

[0107] A herbal cream with high antibacterial effect comprises the following components in parts by weight: 6 parts of geranium extract, 10 parts of thyme extract, 5 parts of peppermint essential oil, 7 parts of honeysuckle extract, 9 parts of chrysanthemum extract, 2.6 parts of glycerol, 0.4 parts of sodium carboxymethylcellulose, 0.3 parts of methyl parahydroxybenzoate, 3 parts of octadecanoic acid, 2.3 parts of emulsifier, 1.5 parts of squalane and 55 parts of water.

[0108] The preparation method of the geranium extract is:

[0109] (1) adding 13% by mass of geranium flowers to YPD medium and sterilizing by high pressure steam to obtain geranium medium;

[0110] (2) The yeast was inoculated into YPD medium and cultured at 33°C and 165 rpm to obtain a seed solution, wherein the yeast content in the seed solution was 5×10 8 CFU / mL;

[0111] (3) inoculating the seed bacterial liquid into the geranium culture medium at a volume ratio of 1:8, culturing at 31° C. and 110 rpm for 24 hours to obtain a fermentation product; subjecting the fermentation product to high-pressure homogenization to obtain a homogenate, centrifuging, and taking a supernatant;

[0112] (4) adding 0.3% by mass of complex enzyme to the supernatant, performing enzymolysis at 46° C. for 3.5 h, inactivating the enzyme at 90° C. for 30 min, and spray drying to obtain a geranium extract.

[0113] High-pressure homogenization: pressure 810Bar, homogenization 3 times.

[0114] Sterilize with high pressure steam at 121℃ for 30min.

[0115] The complex enzyme is composed of alkaline protease, papain and flavor protease in a mass ratio of 0.7:1:1.7.

[0116] The enzyme activity of alkaline protease is 100,000 U / g; the enzyme activity of papain is 50,000 U / g; the enzyme activity of flavor protease is 200,000 U / g.

[0117] The preparation method of the thyme extract is:

[0118] (1) grinding thyme through a 100-mesh sieve to obtain thyme powder, mixing 75 parts by mass of the thyme powder and 230 parts by mass of purified water, and sterilizing to obtain a mixed liquid;

[0119] (2) selecting Lactobacillus brevis, Lactobacillus plantarum, Lactobacillus acidophilus, and Bifidobacterium lactis as mixed bacterial agents; adding 1 part by mass of the mixed bacterial agent and the mixed liquid of step (1) to a fermentation tank, adjusting the pH to 6.3, and performing anaerobically fermentation at 36° C. for 4 days. After the fermentation is completed, sterilization is performed to obtain a fermentation system;

[0120] (3) The fermentation system was centrifuged at 8500 rpm for 12 min, filtered through a 0.22 μm membrane, the supernatant was taken, and spray-dried to obtain a thyme extract.

[0121] The mass ratio of the Lactobacillus brevis, Lactobacillus plantarum, Lactobacillus acidophilus and Bifidobacterium lactis is 2.7:0.9:0.4:1.

[0122] Lactobacillus brevis, bacterial activity 10 billion CFU / g. Collection number: SHBCCD14346. Deposited in Shanghai Microbiological Collection Center.

[0123] Lactobacillus plantarum, bacterial activity 10 billion CFU / g. Collection number: CCTCCAB2010210. Deposited in China Center for Type Culture Collection.

[0124] Lactobacillus acidophilus, bacterial activity 20 billion CFU / g. Collection number: CCTCCAB2010208. Deposited in China Center for Type Culture Collection.

[0125] Bifidobacterium lactis, with an activity of 10 billion CFU / g. Collection number: SHBCCD24675. Deposited in Shanghai Microbiological Collection Center.

[0126] The emulsifier is Tween 60.

[0127] The preparation method of the herbal cream with high antibacterial effect comprises the following steps:

[0128] (1) firstly mix geranium extract, chrysanthemum extract, peppermint essential oil, honeysuckle extract, thyme extract and water, stir evenly, heat to 82° C. and keep warm for 45 minutes to obtain phase A component;

[0129] (2) Mix the remaining components, heat and stir evenly, raise the temperature to 72°C and keep warm for 25 minutes to obtain phase B component;

[0130] (3) Add the phase A component to the phase B component and perform homogenization at a homogenization speed of 12 krpm for 7 min. After the homogenization is completed, stir to obtain a herbal cream with high antibacterial efficiency.

[0131] Comparative Example 3

[0132] The difference between this comparative example and Example 1 is that the composite enzyme is composed of alkaline protease, papain and flavor protease in a mass ratio of 1:1:1.

[0133] A herbal cream with high antibacterial effect comprises the following components in parts by weight: 12 parts of geranium extract, 16 parts of thyme extract, 2 parts of peppermint essential oil, 4 parts of honeysuckle extract, 3 parts of chrysanthemum extract, 2.6 parts of glycerol, 0.4 parts of sodium carboxymethylcellulose, 0.3 parts of methyl parahydroxybenzoate, 3 parts of octadecanoic acid, 2.3 parts of emulsifier, 1.5 parts of squalane and 55 parts of water.

[0134] The preparation method of the geranium extract is:

[0135] (1) adding 13% by mass of geranium flowers to YPD medium and sterilizing by high pressure steam to obtain geranium medium;

[0136] (2) The yeast was inoculated into YPD medium and cultured at 33°C and 165 rpm to obtain a seed solution, wherein the yeast content in the seed solution was 5×10 8 CFU / mL;

[0137] (3) inoculating the seed bacterial liquid into the geranium culture medium at a volume ratio of 1:8, culturing at 31° C. and 110 rpm for 24 hours to obtain a fermentation product; subjecting the fermentation product to high-pressure homogenization to obtain a homogenate, centrifuging, and taking a supernatant;

[0138] (4) adding 0.3% by mass of complex enzyme to the supernatant, performing enzymolysis at 46° C. for 3.5 h, inactivating the enzyme at 90° C. for 30 min, and spray drying to obtain a geranium extract.

[0139] High-pressure homogenization: pressure 810Bar, homogenization 3 times.

[0140] Sterilize with high pressure steam at 121℃ for 30min.

[0141] The complex enzyme is composed of alkaline protease, papain and flavor protease in a mass ratio of 1:1:1.

[0142] The enzyme activity of alkaline protease is 100,000 U / g; the enzyme activity of papain is 50,000 U / g; the enzyme activity of flavor protease is 200,000 U / g.

[0143] The preparation method of the thyme extract is:

[0144] (1) grinding thyme through a 100-mesh sieve to obtain thyme powder, mixing 75 parts by mass of the thyme powder and 230 parts by mass of purified water, and sterilizing to obtain a mixed liquid;

[0145] (2) selecting Lactobacillus brevis, Lactobacillus plantarum, Lactobacillus acidophilus, and Bifidobacterium lactis as mixed bacterial agents; adding 1 part by mass of the mixed bacterial agent and the mixed liquid of step (1) to a fermentation tank, adjusting the pH to 6.3, and performing anaerobically fermentation at 36° C. for 4 days. After the fermentation is completed, sterilization is performed to obtain a fermentation system;

[0146] (3) The fermentation system was centrifuged at 8500 rpm for 12 min, filtered through a 0.22 μm membrane, the supernatant was taken, and spray-dried to obtain a thyme extract.

[0147] The mass ratio of the Lactobacillus brevis, Lactobacillus plantarum, Lactobacillus acidophilus and Bifidobacterium lactis is 2.7:0.9:0.4:1.

[0148] Lactobacillus brevis, bacterial activity 10 billion CFU / g. Collection number: SHBCCD14346. Deposited in Shanghai Microbiological Collection Center.

[0149] Lactobacillus plantarum, bacterial activity 10 billion CFU / g. Collection number: CCTCCAB2010210. Deposited in China Center for Type Culture Collection.

[0150] Lactobacillus acidophilus, bacterial activity 20 billion CFU / g. Collection number: CCTCCAB2010208. Deposited in China Center for Type Culture Collection.

[0151] Bifidobacterium lactis, with an activity of 10 billion CFU / g. Collection number: SHBCCD24675. Deposited in Shanghai Microbiological Collection Center.

[0152] The emulsifier is Tween 60.

[0153] The preparation method of the herbal cream with high antibacterial effect comprises the following steps:

[0154] (1) firstly mix geranium extract, chrysanthemum extract, peppermint essential oil, honeysuckle extract, thyme extract and water, stir evenly, heat to 82° C. and keep warm for 45 minutes to obtain phase A component;

[0155] (2) Mix the remaining components, heat and stir evenly, raise the temperature to 72°C and keep warm for 25 minutes to obtain phase B component;

[0156] (3) Add the phase A component to the phase B component and perform homogenization at a homogenization speed of 12 krpm for 7 min. After the homogenization is completed, stir to obtain a herbal cream with high antibacterial efficiency.

[0157] Comparative Example 4

[0158] The difference between this comparative example and Example 1 is that the mass ratio of Lactobacillus brevis, Lactobacillus plantarum, Lactobacillus acidophilus and Bifidobacterium lactis is 1:1:1:1.

[0159] A herbal cream with high antibacterial effect comprises the following components in parts by weight: 12 parts of geranium extract, 16 parts of thyme extract, 2 parts of peppermint essential oil, 4 parts of honeysuckle extract, 3 parts of chrysanthemum extract, 2.6 parts of glycerol, 0.4 parts of sodium carboxymethylcellulose, 0.3 parts of methyl parahydroxybenzoate, 3 parts of octadecanoic acid, 2.3 parts of emulsifier, 1.5 parts of squalane and 55 parts of water.

[0160] The preparation method of the geranium extract is:

[0161] (1) adding 13% by mass of geranium flowers to YPD medium and sterilizing by high pressure steam to obtain geranium medium;

[0162] (2) The yeast was inoculated into YPD medium and cultured at 33°C and 165 rpm to obtain a seed solution, wherein the yeast content in the seed solution was 5×10 8 CFU / mL;

[0163] (3) inoculating the seed bacterial liquid into the geranium culture medium at a volume ratio of 1:8, culturing at 31° C. and 110 rpm for 24 hours to obtain a fermentation product; subjecting the fermentation product to high-pressure homogenization to obtain a homogenate, centrifuging, and taking a supernatant;

[0164] (4) adding 0.3% by mass of complex enzyme to the supernatant, performing enzymolysis at 46° C. for 3.5 h, inactivating the enzyme at 90° C. for 30 min, and spray drying to obtain a geranium extract.

[0165] High-pressure homogenization: pressure 810Bar, homogenization 3 times.

[0166] Sterilize with high pressure steam at 121℃ for 30min.

[0167] The complex enzyme is composed of alkaline protease, papain and flavor protease in a mass ratio of 0.7:1:1.7.

[0168] The enzyme activity of alkaline protease is 100,000 U / g; the enzyme activity of papain is 50,000 U / g; the enzyme activity of flavor protease is 200,000 U / g.

[0169] The preparation method of the thyme extract is:

[0170] (1) grinding thyme through a 100-mesh sieve to obtain thyme powder, mixing 75 parts by mass of the thyme powder and 230 parts by mass of purified water, and sterilizing to obtain a mixed liquid;

[0171] (2) selecting Lactobacillus brevis, Lactobacillus plantarum, Lactobacillus acidophilus, and Bifidobacterium lactis as mixed bacterial agents; adding 1 part by mass of the mixed bacterial agent and the mixed liquid of step (1) to a fermentation tank, adjusting the pH to 6.3, and performing anaerobically fermentation at 36° C. for 4 days. After the fermentation is completed, sterilization is performed to obtain a fermentation system;

[0172] (3) The fermentation system was centrifuged at 8500 rpm for 12 min, filtered through a 0.22 μm membrane, the supernatant was taken, and spray-dried to obtain a thyme extract.

[0173] The mass ratio of the Lactobacillus brevis, Lactobacillus plantarum, Lactobacillus acidophilus and Bifidobacterium lactis is 1:1:1:1.

[0174] Lactobacillus brevis, bacterial activity 10 billion CFU / g. Collection number: SHBCCD14346. Deposited in Shanghai Microbiological Collection Center.

[0175] Lactobacillus plantarum, bacterial activity 10 billion CFU / g. Collection number: CCTCCAB2010210. Deposited in China Center for Type Culture Collection.

[0176] Lactobacillus acidophilus, bacterial activity 20 billion CFU / g. Collection number: CCTCCAB2010208. Deposited in China Center for Type Culture Collection.

[0177] Bifidobacterium lactis, with an activity of 10 billion CFU / g. Collection number: SHBCCD24675. Deposited in Shanghai Microbiological Collection Center.

[0178] The emulsifier is Tween 60.

[0179] The preparation method of the herbal cream with high antibacterial effect comprises the following steps:

[0180] (1) firstly mix geranium extract, chrysanthemum extract, peppermint essential oil, honeysuckle extract, thyme extract and water, stir evenly, heat to 82° C. and keep warm for 45 minutes to obtain phase A component;

[0181] (2) Mix the remaining components, heat and stir evenly, raise the temperature to 72°C and keep warm for 25 minutes to obtain phase B component;

[0182] (3) Add the phase A component to the phase B component and perform homogenization at a homogenization speed of 12 krpm for 7 min. After the homogenization is completed, stir to obtain a herbal cream with high antibacterial efficiency.

[0183] Comparative Example 5

[0184] The difference between this comparative example and Example 1 is that the geranium extract and the thyme extract are commercially available from Lanzhou Waterless Biotechnology Co., Ltd.

[0185] A herbal cream with high antibacterial effect comprises the following components in parts by weight: 12 parts of geranium extract, 16 parts of thyme extract, 2 parts of peppermint essential oil, 4 parts of honeysuckle extract, 3 parts of chrysanthemum extract, 2.6 parts of glycerol, 0.4 parts of sodium carboxymethylcellulose, 0.3 parts of methyl parahydroxybenzoate, 3 parts of octadecanoic acid, 2.3 parts of emulsifier, 1.5 parts of squalane and 55 parts of water.

[0186] The emulsifier is Tween 60.

[0187] The preparation method of the herbal cream with high antibacterial effect comprises the following steps:

[0188] (1) firstly mix geranium extract, chrysanthemum extract, peppermint essential oil, honeysuckle extract, thyme extract and water, stir evenly, heat to 82° C. and keep warm for 45 minutes to obtain phase A component;

[0189] (2) Mix the remaining components, heat and stir evenly, raise the temperature to 72°C and keep warm for 25 minutes to obtain phase B component;

[0190] (3) Add the phase A component to the phase B component and perform homogenization at a homogenization speed of 12 krpm for 7 min. After the homogenization is completed, stir to obtain a herbal cream with high antibacterial efficiency.

[0191] Performance Testing

[0192] 70 people aged 18 to 45 with skin problems were selected as volunteers. Skin problems refer to rashes and itching on the skin. They were divided into 7 groups, with 10 people in each group.

[0193] During the test, in addition to normal skin care, the herbal cream was applied once in the morning and once in the evening, with the amount applied each time not exceeding 1g. During the application period, if more severe rashes, itching or other discomforts occur, stop immediately and record the number of people who experience discomfort. After 14 consecutive days of application, observe the skin condition and compare it with the skin before the test to score the performance of the herbal cream.

[0194] The scoring criteria are as follows: 1. Improvement effect of rash and itching: no obvious change in rash and itching 0-3 points; rash and itching improved but not completely disappeared 4-7 points; rash and itching basically disappeared 8-10 points. 2. Moisturizing effect: no obvious discomfort during use, the skin still has moisturizing effect within 2 hours after the test is recorded as A; dryness, tightness, itching and other discomfort symptoms during use are recorded as B. The results are shown in Table 1.

[0195] Table 1 Performance test results

[0196]

[0197] The results show that the herbal creams prepared in Examples 1 to 2 have good antibacterial effect, good moisturizing effect, and are mild and non-irritating.

[0198] The above is a preferred embodiment of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principles of the present invention. These improvements and modifications should also be regarded as the scope of protection of the present invention.

Claims

1. A herbal cream with high antibacterial effect, characterized in that: The invention comprises the following components in parts by weight: 10-13 parts of geranium extract, 15-17 parts of thyme extract, 1-3 parts of peppermint essential oil, 3-6 parts of honeysuckle extract, 1-4 parts of chrysanthemum extract, 2-3 parts of glycerol, 0.2-0.5 parts of sodium carboxymethyl cellulose, 0.2-0.6 parts of methyl parahydroxybenzoate, 2-4 parts of octadecanoic acid, 2-3 parts of emulsifier, 1.2-1.6 parts of squalane and 50-60 parts of water.

2. The herbal cream with high antibacterial effect according to claim 1, characterized in that: The preparation method of the geranium extract is: (1) adding 12-15% by mass of geranium flowers to YPD medium and sterilizing by high pressure steam to obtain geranium medium; (2) The yeast was inoculated into YPD medium and cultured at 32-35°C and 160-170 rpm to obtain a seed solution, wherein the yeast content in the seed solution was 3×10 8 -6×10 8 CFU / mL; (3) inoculating the seed bacterial liquid into the geranium culture medium at a volume ratio of 1:7-9, culturing at 30-32° C. and 100-120 rpm for 22-25 hours to obtain a fermentation product; subjecting the fermentation product to high-pressure homogenization to obtain a homogenate, centrifuging, and taking a supernatant; (4) adding 0.2-0.3% by mass of complex enzyme to the supernatant, performing enzymolysis at 45-48° C. for 3-4 h, inactivating the enzyme at 90° C. for 30 min, and spray drying to obtain a geranium extract.

3. The herbal cream with high antibacterial effect according to claim 2, characterized in that: High-pressure homogenization: pressure 800-820Bar, homogenization 3 times.

4. The herbal cream with high antibacterial effect according to claim 2, characterized in that: The complex enzyme is composed of alkaline protease, papain and flavor protease in a mass ratio of 0.5-0.8:1:1.6-1.

9.

5. The herbal cream with high antibacterial effect according to claim 2, characterized in that: The enzyme activity of alkaline protease is 100,000 U / g; the enzyme activity of papain is 50,000 U / g; the enzyme activity of flavor protease is 200,000 U / g.

6. The herbal cream with high antibacterial effect according to claim 1, characterized in that: The preparation method of the thyme extract is: (1) grinding thyme through a 100-mesh sieve to obtain thyme powder, mixing 70-80 parts by weight of the thyme powder with 200-300 parts by weight of purified water, and sterilizing to obtain a mixed liquid; (2) selecting Lactobacillus brevis, Lactobacillus plantarum, Lactobacillus acidophilus, and Bifidobacterium lactis as mixed bacterial agents; adding 1 part by mass of the mixed bacterial agent and the mixed liquid of step (1) to a fermentation tank, adjusting the pH to 6.3, and performing anaerobically fermentation at 36° C. for 3-5 days. After the fermentation is completed, sterilization is performed to obtain a fermentation system; (3) The fermentation system was centrifuged at 8000-9000 rpm for 10-15 min, filtered through a 0.22 μm membrane, the supernatant was taken, and spray-dried to obtain a thyme extract.

7. The herbal cream with high antibacterial effect according to claim 6, characterized in that: The mass ratio of the Lactobacillus brevis, Lactobacillus plantarum, Lactobacillus acidophilus and Bifidobacterium lactis is 2.5-3:0.8-1.2:0.3-0.6:0.7-1.

8. The herbal cream with high antibacterial effect according to claim 6, characterized in that: Lactobacillus brevis, active 10 billion CFU / g, deposit number: SHBCCD14346; Lactobacillus plantarum, active 10 billion CFU / g, deposit number: CCTCCAB2010210; Lactobacillus acidophilus, with an activity of 20 billion CFU / g; Collection number: CCTCCAB2010208; Bifidobacterium lactis, bacterial activity 10 billion CFU / g, collection number: SHBCCD24675.

9. The method for preparing the herbal cream with high antibacterial effect according to any one of claims 1 to 8, characterized in that: The following steps are involved: (1) firstly mix geranium extract, chrysanthemum extract, peppermint essential oil, honeysuckle extract, thyme extract and water, stir evenly, heat to 80-85° C. and keep warm for 40-50 min to obtain phase A component; (2) Mix the remaining components, heat and stir evenly, raise the temperature to 70-75°C and keep warm for 20-30 minutes to obtain phase B component; (3) Add the phase A component to the phase B component and perform homogenization at a homogenization speed of 11-12 krpm for 6-8 min. After the homogenization is completed, stir to obtain a herbal cream with high antibacterial efficiency.

10. Use of the herbal cream with high antibacterial effect according to any one of claims 1 to 8.

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