Antibacterial anti-inflammatory blood vessel promoting time sequence microneedle and preparation method thereof

Abstract

The invention relates to an antibacterial anti-inflammatory blood vessel promoting time sequence microneedle and a preparation method thereof. The microneedle comprises a first microneedle mold, a second microneedle mold and a third microneedle mold which are arranged in sequence, the first microneedle mold is filled with a carrier material A, an antibacterial component and / or a traditional Chinese medicine component A; the second microneedle mold is filled with a carrier material B, an anti-inflammatory component and / or a traditional Chinese medicine component B; and the third microneedle mold is filled with a carrier material C and / or a vascular tissue regeneration promoting component. A good microenvironment is provided for tissue regeneration, the microneedle sequentially resists bacteria, resists inflammation and promotes vascular tissue regeneration, the microneedle conforming to the time sequence is beneficial to tissue regeneration, and the microneedle has a good application prospect.

Description

Technical Field

[0001] The present invention belongs to the technical field of microneedles, and in particular relates to an antibacterial, anti-inflammatory and angiogenic time-sequential microneedle and a preparation method thereof. Background Art

[0002] Painless transdermal drug delivery by microneedles is a new drug delivery technology. Its working principle is to painlessly create micron-level drug delivery channels on the skin to achieve effective absorption of drugs by subcutaneous tissue. It has the advantages of being painless, safe, and easy to operate. Microneedles can be classified into hollow microneedles, soluble microneedles, coated microneedles, solid microneedles, etc. according to the different structures of the needles. Among them, soluble microneedles are microneedles prepared by mixing drugs into microneedle mother liquid with biodegradable materials as the matrix, such as hyaluronic acid, polylactic acid, etc. After the soluble microneedles penetrate the skin, they dissolve in the body to release the drugs.

[0003] In addition to the advantages of general microneedles, soluble microneedles use their own degradable properties to solve the problem of microneedles breaking in the skin, which is difficult to deal with. Relatively speaking, they also increase the drug loading capacity of microneedles and expand the application range of microneedles. Therefore, soluble microneedles are expected to become an ideal carrier for transdermal drug delivery systems and have very good application value.

[0004] However, current soluble microneedles can only deliver a single drug and cannot achieve transdermal delivery of multiple drugs, which affects their further application expansion. Summary of the invention

[0005] The technical problem to be solved by the present invention is to provide an antibacterial, anti-inflammatory and angiogenic time-sequence microneedle and a preparation method thereof. The microneedle provides a good microenvironment for tissue regeneration, and is antibacterial, anti-inflammatory and promotes vascular tissue regeneration in sequence. The microneedle in accordance with the time sequence is helpful for tissue regeneration and has good application prospects.

[0006] The present invention provides an antibacterial, anti-inflammatory and angiogenic time-sequential microneedle, comprising a first microneedle mold, a second microneedle mold and a third microneedle mold which are arranged in sequence; the first microneedle mold is filled with a carrier material A, an antibacterial component and / or a traditional Chinese medicine component A; the second microneedle mold is filled with a carrier material B, an anti-inflammatory component and / or a traditional Chinese medicine component B; the third microneedle mold is filled with a carrier material C and / or a component promoting angiogenesis.

[0007] Preferably, the carrier material A is low-concentration PEG or gelatin; the antibacterial component is an antibiotic or an antibacterial inorganic ion; and the traditional Chinese medicine component A is chitosan.

[0008] Preferably, the low concentration refers to a carrier material such as PEG or gelatin with a mass volume fraction of 5%-20%, which has a fast degradation rate.

[0009] Preferably, the antibacterial inorganic ions are silver ions or zinc ions.

[0010] Preferably, the carrier material B is a high-concentration PEG or gelatin or a carrier material with a slow degradation rate; the anti-inflammatory component is a hormone or an anti-inflammatory inorganic ion; and the traditional Chinese medicine component B is curcumin, gallic acid or icariin.

[0011] Preferably, the high concentration refers to carrier materials such as PEG or gelatin with a mass volume fraction in the range of 20%-30%, and the carrier material with a slow degradation rate refers to high molecular polymers such as polycaprolactone (PCL), polyurethane (PU) and the like.

[0012] Preferably, the anti-inflammatory inorganic ions are copper ions or magnesium ions.

[0013] Preferably, the carrier material C is a carrier material with a higher concentration of PEG, gelatin or a carrier material with a slower degradation rate.

[0014] Preferably, the higher concentration refers to carrier materials such as PEG or gelatin with a mass volume fraction greater than 30%, and the carrier material with a slower degradation rate refers to high molecular polymers such as polycaprolactone (PCL), polyurethane (PU), etc.

[0015] Preferably, the growth factor is VEGF or bFGF.

[0016] Preferably, the growth-promoting inorganic ions are copper ions or magnesium ions.

[0017] The present invention also provides a method for preparing an antibacterial, anti-inflammatory and angiogenic time-sequential microneedle, comprising the following steps:

[0018] A first microneedle mold, a second microneedle mold and a third microneedle mold arranged in parallel are prepared; carrier material A, antibacterial component and / or Chinese medicine component A are poured into the first microneedle mold; carrier material B, anti-inflammatory component and / or Chinese medicine component B are poured into the second microneedle mold; and a vascular tissue regeneration promoting component is poured into the third microneedle mold to obtain antibacterial, anti-inflammatory and vascular pro-timed microneedles.

[0019] Beneficial Effects

[0020] The present invention provides a good microenvironment for tissue regeneration, and sequentially has antibacterial, anti-inflammatory, and promotes vascular tissue regeneration. The microneedles in accordance with the time sequence are helpful for tissue regeneration, and have good application prospects. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] Figure 1 It is a schematic diagram of the structure of the present invention; wherein, 1 is the first microneedle mold, 2 is the second microneedle mold, and 3 is the third microneedle mold. DETAILED DESCRIPTION

[0022] The present invention will be further described below in conjunction with specific embodiments. It should be understood that these embodiments are only used to illustrate the present invention and are not intended to limit the scope of the present invention. In addition, it should be understood that after reading the content taught by the present invention, those skilled in the art can make various changes or modifications to the present invention, and these equivalent forms fall within the scope limited by the appended claims of the application equally.

[0023] Example 1

[0024] like Figure 1 As shown, this embodiment provides an antibacterial, anti-inflammatory and angiogenic timing microneedle, comprising a first microneedle mold, a second microneedle mold and a third microneedle mold arranged in sequence; the first microneedle mold is filled with PEG (5% by volume), antibiotics and chitosan; the second microneedle mold is filled with PEG (20% by volume), hormones and curcumin; the third microneedle mold is filled with PEG (35% by volume) and growth factor VEGF.

[0025] Example 2

[0026] like Figure 1 As shown, this embodiment provides an antibacterial, anti-inflammatory and angiogenic timing microneedle, comprising a first microneedle mold, a second microneedle mold and a third microneedle mold arranged in sequence; the first microneedle mold is filled with gelatin (mass volume fraction 5%), silver ions and chitosan; the second microneedle mold is filled with gelatin (mass volume fraction 20%), copper ions and gallic acid; the third microneedle mold is filled with gelatin (mass volume fraction 35%) and growth factor VEGF.

[0027] Example 3

[0028] like Figure 1 As shown, this embodiment provides an antibacterial, anti-inflammatory and angiogenic timing microneedle, comprising a first microneedle mold, a second microneedle mold and a third microneedle mold arranged in sequence; the first microneedle mold is filled with PEG (5% by volume), antibiotics and chitosan; the second microneedle mold is filled with gelatin (20% by volume), hormones and icariin; the third microneedle mold is filled with PCL (polycaprolactone) and magnesium ions.

[0029] The antibacterial, anti-inflammatory and angiogenic time-sequence microneedles described in Examples 1-3 provide a good microenvironment for tissue regeneration, and sequentially perform antibacterial, anti-inflammatory and angiogenic functions. Microneedles that conform to the time sequence are helpful for tissue regeneration and have good application prospects.

Claims

1. An antibacterial, anti-inflammatory and angiogenic microneedle, characterized by: It comprises a first microneedle mold, a second microneedle mold and a third microneedle mold which are arranged in sequence; the first microneedle mold is loaded with a carrier material A, an antibacterial component and / or a Chinese medicine component A; the second microneedle mold is loaded with a carrier material B, an anti-inflammatory component and / or a Chinese medicine component B; the third microneedle mold is loaded with a carrier material C and / or a component promoting vascular tissue regeneration.

2. The antibacterial, anti-inflammatory and angiogenic microneedle according to claim 1, characterized in that: The carrier material A is low-concentration PEG or gelatin; the antibacterial component is antibiotics or antibacterial inorganic ions; and the traditional Chinese medicine component A is chitosan.

3. The antibacterial, anti-inflammatory and angiogenic microneedle according to claim 2, characterized in that: The antibacterial inorganic ions are silver ions or zinc ions.

4. The antibacterial, anti-inflammatory and angiogenic microneedle according to claim 1, characterized in that: The carrier material B is high-concentration PEG, gelatin or a carrier material with a slow degradation rate; the anti-inflammatory component is a hormone or an anti-inflammatory inorganic ion; and the traditional Chinese medicine component B is curcumin, gallic acid or icariin.

5. The antibacterial, anti-inflammatory and angiogenic microneedle according to claim 4, characterized in that: The anti-inflammatory inorganic ions are copper ions or magnesium ions.

6. The antibacterial, anti-inflammatory and angiogenic microneedle according to claim 1, characterized in that: The carrier material C is PEG, gelatin with a higher concentration or a carrier material with a slower degradation rate; the component promoting vascular tissue regeneration is a growth factor or an inorganic ion that promotes growth.

7. The antibacterial, anti-inflammatory and angiogenic microneedle according to claim 6, characterized in that: The growth factor is VEGF or bFGF; the inorganic ions promoting growth are copper ions or magnesium ions.

8. A method for preparing the antibacterial, anti-inflammatory and angiogenic microneedle according to claim 1, comprising the following steps: A first microneedle mold, a second microneedle mold and a third microneedle mold arranged in parallel are prepared; carrier material A, antibacterial component and / or Chinese medicinal component A are poured into the first microneedle mold; carrier material B, anti-inflammatory component and / or Chinese medicinal component B are poured into the second microneedle mold; carrier material C and / or a component promoting vascular tissue regeneration are poured into the third microneedle mold to obtain antibacterial, anti-inflammatory and pro-vascular sequential microneedles.

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