Grinding device for biological tissue

Abstract

The invention provides a grinding device for biological tissues, and belongs to the technical field of biological tissue grinding. In order to solve the problems that an existing biological tissue grinding device is poor in use effect, and a centrifugal tube is broken due to direct impact or scraping of a grinding head in the grinding process, the grinding device for the biological tissue comprises a centrifugal tube fixing base and a grinder arranged on the centrifugal tube fixing base; the grinder comprises a grinding mechanism, a low-temperature stirring mechanism and a protective cover arranged at the end part of the low-temperature stirring mechanism, during use, the contact position of the grinding mechanism and the centrifugal tube can be protected through the protective cover, the grinding mechanism is prevented from being in direct contact with the inner wall of the centrifugal tube, and therefore the situation that the centrifugal tube is broken due to direct impact or scraping of the grinding head in the grinding process is avoided.

Description

Technical Field

[0001] The invention belongs to the technical field of biological tissue grinding, and in particular relates to a grinding device for biological tissue. Background Art

[0002] In the fields of biological science research, medical diagnosis, and biotechnology, the processing and analysis of biological tissues is an extremely common and critical link. Traditional biological tissue grinding methods often have many limitations. For example, when using simple tools for grinding, it is difficult to accurately control the grinding force and degree, which can easily lead to over-grinding or insufficient grinding of tissues, affecting the accuracy of subsequent experimental results. At the same time, the lack of an effective protection mechanism during the grinding process often causes the centrifuge tube to break due to direct impact or scratching by the grinding head during the grinding process, which not only causes sample loss, but may also cause safety hazards. In addition, if the heat generated by grinding cannot be dissipated in time, it will cause protein denaturation and enzyme inactivation in biological tissues, change the physiological characteristics of the tissue, and fail to truly reflect its original state, thereby affecting the in-depth research and application of biological tissues.

[0003] The patent with publication number CN219111786U discloses a tissue sample cryogenic grinding device, comprising: an adapter and a centrifuge tube, wherein zirconium oxide grinding balls are placed in the centrifuge tube; the adapter is a box structure, and its internal space is divided into a top cavity located at the top and a low-temperature holding liquid storage chamber located at the bottom by a partition layer; the low-temperature holding liquid storage chamber is provided with a holding liquid addition port with a rubber sealing plug I; the partition layer is provided with a placement through hole, the centrifuge tube is clamped in the placement through hole, and the lower end extends to the holding liquid storage chamber; the top cavity is equipped with a top cover, and a vibration rod with a rubber sealing plug II is vertically arranged at the bottom of the top cover; the vibration rod extends into the centrifuge tube, and the rubber sealing plug II thereon seals the tube mouth of the centrifuge tube, and the zirconium oxide grinding balls in the centrifuge tube are located in the gap between the inner wall of the centrifuge tube and the vibration rod. When in use, when the tissue sample low-temperature grinding device is fixed on the three-dimensional vibrating ball mill, the adapter and the top cover can be brought into contact with the vibrating part of the three-dimensional vibrating ball mill. Through vibration transmission, the zirconia grinding balls and the vibrating rod are both vibrating. Compared with the prior art in which only the zirconia grinding balls in the centrifuge tube vibrate, the zirconia grinding balls and the vibrating rod can interact with each other to improve the impact grinding effect on the tissue sample in the centrifuge tube; the lower section of the centrifuge tube is located in an additional low-temperature preservation liquid storage chamber, which can promptly dissipate the heat generated during the grinding impact into the low-temperature preservation liquid.

[0004] However, the above technical solution performs grinding by vibrating the zirconium oxide grinding balls arranged in the centrifuge tube. After repeated use, the centrifuge tube is broken due to direct impact or scratching by the zirconium oxide grinding balls during the grinding process. In addition, the above technical solution requires flushing the centrifuge tube, the zirconium oxide grinding balls and the vibration rod in the subsequent flushing process to recover the biological tissue, which is cumbersome to operate. Summary of the invention

[0005] The invention aims to overcome the problem that the existing biological tissue grinding device has poor use effect and the centrifuge tube is broken due to direct impact or scraping by the grinding head during the grinding process.

[0006] In order to achieve the above-mentioned purpose, the technical idea adopted by the present invention is: a grinding device for biological tissue is provided, including a centrifuge tube fixed base and a grinder arranged on the centrifuge tube fixed base, the grinder includes a grinding mechanism, a low-temperature stirring mechanism, and a protective cover arranged at the end of the low-temperature stirring mechanism; wherein the low-temperature stirring mechanism is sleeved with the grinding mechanism, and a reset spring is provided between the low-temperature stirring mechanism and the grinding mechanism; in this way, when in use, the position where the grinding mechanism contacts the centrifuge tube can be protected by the protective cover to prevent the grinding mechanism from directly contacting the inner wall of the centrifuge tube, thereby avoiding the centrifuge tube from being broken due to direct impact or scratching by the grinding head during the grinding process; in addition, after the grinding is completed, the protective cover automatically turns over under the limiting action of the grinding head in the grinding mechanism, thereby facilitating the subsequent flushing process to flush the tissue remaining on the protective cover into the centrifuge tube, thereby realizing the recovery of biological samples.

[0007] Based on the above technical ideas, the technical solution adopted by the present invention is: A grinding device for biological tissue, comprising: Centrifuge tube base; The outer shell is arranged above the centrifuge tube base and connected to the centrifuge tube base through a lifting rod; A fixed column is arranged on the top of the centrifuge tube base, and a centrifuge tube is arranged inside the fixed column; A grinding mechanism, the upper end of which is slidably connected to the housing and the lower end of which is located inside the centrifuge tube; A low temperature stirring mechanism, the upper end of which is located inside the outer shell and connected to the outer shell; The protective cover is a hemispherical structure and is connected to the lower end of the low-temperature stirring mechanism.

[0008] In the above technical solution, preferably, the grinding mechanism includes: Hollow shaft motor; A push ring is sleeved on the outer surface of the hollow shaft motor, and the inner surface of the push ring is connected to the outer side of the hollow shaft motor, and the outer surface of the push ring is slidably connected to the inner wall of the opening of the shell; Two grinding rods are located between the push ring and the protective cover, and the upper end of each grinding rod is connected to the lower surface of the hollow shaft motor, and the lower end is provided with a grinding ball.

[0009] To further limit the above technical solution, the low temperature stirring mechanism includes: An electric push rod, the fixed end of which is connected to the housing, and a piston is provided at the end of the push rod portion of the electric push rod; The upper end of the liquid storage tube is connected with the piston sleeve, and the lower end passes through the hollow shaft motor and is connected with the protective cover, and the lower end of the liquid storage tube is located between the two grinding rods; A liquid outlet pipe, the upper end of which is connected to the liquid storage pipe, the lower end of which passes through the hollow shaft of the hollow shaft motor and is connected to the protective cover, and a plurality of guide pipes are arranged on the lower end of the liquid outlet pipe; The mixing drum is sleeved on the lower end of the liquid outlet pipe and is rotatably connected with the liquid outlet pipe.

[0010] The above technical solution is further limited in that a spring is sleeved on the liquid outlet pipe, and the upper end of the spring is connected to the bottom surface of the liquid storage pipe, and the lower end is connected to the upper surface of the hollow shaft motor.

[0011] The above technical solution is further limited, and the mixing drum comprises: Mixing drum body; A plurality of rotating blades are arranged at equal intervals on the side surface of the mixing drum body.

[0012] The above technical solution is further limited in that the mixing drum body is also provided with a plurality of stirring rods for stirring the biological tissue located between the two grinding balls.

[0013] The above technical solution is further limited in that a stepped groove with an opening facing upward is provided on the fixing column.

[0014] The above technical solution is further limited, and the stepped groove includes: A first circular groove is located on the top surface of the fixing column; The second circular groove is located at the lower end of the first circular groove and is communicated with the first circular groove; the upper surface of the second circular groove is in contact with the lower surface of the push ring.

[0015] The above technical solution is further limited in that a convex strip is provided on the outer surface of the push ring, and a sliding groove matching and slidably connected with the convex strip is provided at the opening of the shell (==). Beneficial Effects

[0016] 1. The present invention adopts a combined design of a grinding mechanism, a protective cover and a low-temperature stirring mechanism. During the grinding process, the protective cover can be used to protect the position where the grinding mechanism contacts the centrifuge tube to prevent the grinding mechanism from directly contacting the inner wall of the centrifuge tube, thereby preventing the centrifuge tube from being broken due to direct impact or scratching by the grinding head during the grinding process. At the same time, the biological tissue in the centrifuge tube can be stirred by the low-temperature stirring mechanism to make the stirring more sufficient.

[0017] 2. The present invention combines the design of a spring, an anti-slip cover and a grinding mechanism. After grinding is completed, the spring located in the shell is no longer squeezed by the fixed column below the shell, and the spring returns to a natural state, and then the spring pushes the push block downward, thereby driving the two grinding rods to move downward, causing the grinding balls at the lower ends of the grinding rods to move downward, so that the grinding balls press downward on the two ends of the protective cover, causing the protective cover to flip from a bowl-shaped shape to an inverted bowl-shaped shape, so that when the ground tissue in the centrifuge tube is subsequently flushed with liquid, the tissue remaining on the protective cover can be flushed into the centrifuge tube to achieve the recovery of biological samples.

[0018] 3. The present invention adopts the combined design of the liquid storage tube, the liquid outlet tube, the guide tube and the rotating blades. The cooling liquid in the liquid storage tube used to cool the biological tissue in the centrifuge tube flows out from the liquid storage tube to the guide tube, and then the cooling liquid flows out from the outlet of the guide tube and falls on the rotating blades, so that the cooling liquid generates thrust on the rotating blades, causing the rotating blades to rotate, thereby achieving stirring of the biological tissue during the grinding process; Fourth, the present invention combines the design of the rotating blades and the stirring rod, so that the stirring tank can further stir the biological tissue in the gap between the grinding balls of the two grinding rods, making the stirring more sufficient and uniform. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0020] Figure 1 A schematic structural diagram of a grinding device for biological tissue provided by an embodiment of the present invention; Figure 2 for Figure 1 The enlarged view of point A in the middle; Figure 3 for Figure 1 A schematic diagram of the structure of the device shown in another perspective; Figure 4 for Figure 3 The enlarged view of point B in the middle; Figure 5 for Figure 1 A half-section view of the device shown; Figure 6 for Figure 5 Enlarged view of point C in the middle; Figure 7 for Figure 5 The enlarged view of point D in the middle; Figure 8 This is the usage status diagram of the grinder; Fig. 9 for Figure 8 Status diagram of the medium grinding mechanism, low temperature stirring mechanism and centrifuge tube.

[0021] Fig.10 for Fig. 9 State diagram of the medium grinding mechanism and low temperature stirring mechanism; Fig.11 for Fig.10 Enlarged view of point E in the middle; Fig.12 This is a diagram of the grinder extending into the bottom of the centrifuge tube; Fig.13 for Fig.12 State diagram of the medium grinding mechanism and low temperature stirring mechanism; Fig.14 for Fig.13 A magnified view of the structure at F in the middle; Fig.15 for Figure 1 A diagram showing the device in use; Fig.16 for Fig.15 a cross-sectional view of the disc of the device shown; Fig.17 for Fig.16 Enlarged view of point G in the middle.

[0022] Among them, 1. shell; 2. protective cover; 3. Grinding mechanism; 31. Hollow shaft motor; 32. Push ring; 32a. Raised strip; 33. Grinding rod; 33a. Grinding ball; 4. low temperature stirring mechanism; 41. electric push rod; 41a. piston; 42. liquid storage tube; 43. liquid outlet tube; 43a. guide tube; 44. stirring drum; 44a. stirring drum body; 44b. rotating blade; 44c. stirring stick; 5. Lifting rod; 6. Centrifuge tube base; 61. Fixed column; 62. Stepped groove; 62a. First circular groove; 62b. Second circular groove; 7. Centrifuge tube; 8. Spring. DETAILED DESCRIPTION

[0023] To make the purpose, technical solution and advantages of the embodiments of the present invention more clear, the technical solution in the embodiments of the present invention will be clearly and completely described below in conjunction with the accompanying drawings in the embodiments of the present invention.

[0024] In the description of the present invention, it is necessary to understand that the terms "up", "down", "left", "right", "top", "bottom", "inside", "outside", etc. indicate orientations or positional relationships based on the orientations or positional relationships shown in the accompanying drawings, and are only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as a limitation on the present invention.

[0025] In addition, features limited to "first" and "second" may include one or more of the features explicitly or implicitly. In the description of the present invention, unless otherwise specified, "plurality" means two or more.

[0026] In the description of the present invention, it should be noted that, unless otherwise clearly specified and limited, the terms "installed", "connected", and "connected" should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection, or it can be indirectly connected through an intermediate medium, or it can be the internal communication of two components. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0027] The inventors have found that the current biological tissue grinding device has the following problems when in use: 1. The grinding device contacts the inner wall of the centrifuge tube. After repeated use, the centrifuge tube is broken due to direct impact or scratching by the zirconium oxide grinding balls during the grinding process; 2. In the subsequent flushing process to recover the biological tissue, the centrifuge tube, zirconium oxide grinding balls and vibration rods need to be flushed, which is cumbersome.

[0028] Based on the above findings, the present application provides a grinding device for biological tissue, including a centrifuge tube fixed base 6 and a grinder arranged on the centrifuge tube fixed base 6, the grinder including a grinding mechanism 3, a low-temperature stirring mechanism 4, and a protective cover 2 arranged at the end of the low-temperature stirring mechanism 4; wherein the low-temperature stirring mechanism 4 is sleeved with the grinding mechanism 3, and a reset spring 8 is provided between the low-temperature stirring mechanism 4 and the grinding mechanism 3; thus, the present application 1. places the protective cover 2 at a position where the grinding mechanism 3 contacts the centrifuge tube 7 to prevent the grinding mechanism 3 from directly contacting the inner wall of the centrifuge tube 7, thereby avoiding the centrifuge tube 7 from being broken during the grinding process due to direct impact or scratching by the grinding device, such as zirconium oxide grinding balls; 2. after the grinding is completed, the protective cover 2 automatically turns over under the limiting action of the grinding rod 33 in the grinding mechanism 3, thereby facilitating the subsequent flushing process to flush the biological tissue remaining on the protective cover 2 into the centrifuge tube 7, thereby realizing the recovery of the biological sample. Example 1

[0029] This embodiment provides a grinding device for biological tissue, such as Figure 1-17 As shown, it includes a centrifuge tube base 6, a shell 1, and a grinding mechanism 3 and a low-temperature stirring mechanism 4 arranged in the shell 1; wherein a protective cover 2 is provided at the lower end of the low-temperature stirring mechanism 4.

[0030] like Figure 1 and 2 As shown, a fixing column 61 for fixing the centrifuge tube is provided on the centrifuge tube base 6, and a stepped groove 62 with an opening facing upward is formed on the fixing column 61; The stepped groove 62 includes a first circular groove 62 a and a second circular groove 62 b connected to the first circular groove 62 ; wherein the first circular groove 62 a is used for matching and connecting with the housing 1 , and the second circular groove 62 b is used for fixing the centrifuge tube 7 .

[0031] The housing 1 is a cylindrical structure with an opening facing downwards; the side of the housing 1 is connected to the bottom plate of the centrifuge tube base 6 through a lifting rod 5. The lifting rod 5 is an electric push rod, and the push rod part of the lifting rod 5 is an inverted L-shaped structure.

[0032] In practical applications, the end of the push rod portion of the lifting rod 5 is fixedly connected to the side of the housing 1. It should be noted that the embodiment of the present invention does not limit the fixed connection method of the end of the push rod portion of the lifting rod 5 and the housing 1. For example, the end of the push rod portion of the lifting rod 5 is integrally connected to the housing.

[0033] The grinding mechanism 3 includes a hollow shaft motor 31, a push ring 32 and a grinding rod 33; wherein, a grinding ball 33a is provided at the end of the grinding rod 33; it should be noted that the hollow shaft motor 31 provided in the embodiment of the present invention is the prior art, and the embodiment of the present invention does not make any improvements thereto. For example, the model of the hollow shaft motor 31 is: TODO 29BM16.

[0034] Specifically, Figure 4 and 6 As shown, the lower surface of the hollow shaft motor 31, that is, the rotating surface of the hollow shaft motor 31, is connected to two grinding rods 33 arranged parallel to each other; the embodiment of the present invention does not limit the connection method between the hollow shaft motor 31 and the two grinding rods 33. For example, the output end of the hollow shaft motor 31 is welded or bolted to the two grinding rods 33 through a transverse connecting rod. In actual use, each grinding rod 33 rotates under the drive of the hollow shaft motor 31, and then the grinding ball 33a at the lower end of the grinding rod 33 grinds the biological tissue in the centrifuge tube 7.

[0035] The push ring 32 is a circular ring structure with a groove on the bottom surface. The hollow shaft motor 31 is arranged in the groove of the push ring 32, and the inner wall of the groove of the push ring 32 is fixedly connected to the hollow shaft motor 31. The embodiment of the present invention does not limit the specific connection method between the push ring 32 and the hollow shaft motor 31. For example, the inner wall of the groove of the push ring 32 is fixedly connected to the hollow shaft motor 31 by gluing or bolts.

[0036] Among them, a convex strip 32a is vertically arranged on the outer surface of the push ring 32; correspondingly, a slide groove at the opening of the shell 1 is arranged on the inner edge of the opening of the shell 1; in this way, when in use, the convex strip 32a of the push ring 32 is located in the slide groove at the opening of the shell 1, so that the push ring 32 can be prevented from rotating in the shell 1, thereby improving the stability of the grinding ball 33a.

[0037] In practical applications, after the biological tissue is placed in the centrifuge tube 7, the housing 1 needs to be closely matched with the fixing column 61 to grind the biological tissue; in this way, when the housing 1 is driven by the lifting rod 5 and moves downward into the first circular groove 62a, as shown in FIG. Fig.17 As shown, the push ring 32 is located in the first circular groove 62a and contacts the inner bottom surface of the first circular groove 62a until the shell 1 continues to enter the first circular groove 62a. At this time, the push ring 32 enters into the opening of the shell 1 under the action of the inner bottom surface of the first circular groove 62a.

[0038] The lower end of the low-temperature stirring mechanism 4 passes through the central through hole of the hollow shaft motor 31 and is located between the two grinding rods 33 so as to stir the biological tissue in the centrifuge tube 7 .

[0039] like Figures 5 to 7 As shown, the low temperature stirring mechanism 4 includes an electric push rod 41, a liquid storage pipe 42, a liquid outlet pipe 43 and a stirring drum 44. The bottom end of the liquid outlet pipe 43 is connected to the protective cover 2.

[0040] Among them, the fixed end of the electric push rod 41 is located in the opening of the shell 1, and the upper end of the electric push rod 41, that is, the fixed end of the electric push rod is fixedly connected to the inner wall of the shell 1; the lower end of the electric push rod 41, that is, the push rod end of the electric push rod 41 is provided with a piston 41a.

[0041] The liquid storage tube 42 is a cylindrical structure with two upper and lower openings, and the upper and lower openings are connected. The piston 41a is located in the upper opening of the liquid storage tube 42, and the piston 41a is tightly connected to the inner wall of the liquid storage tube 42 so as to squeeze the coolant in the liquid storage tube 42 downward; the lower opening of the liquid storage tube 42 is connected to the liquid outlet pipe 43. The liquid storage tube 42 is fixedly connected to the inner wall of the housing 1 through a connecting rod.

[0042] Specifically, the liquid outlet pipe 43 is a cylindrical structure, the upper end of the liquid outlet pipe 43 is connected to the lower end opening of the liquid storage pipe 42, and the lower end of the liquid outlet pipe 43 is provided with a plurality of guide pipes 43a, which are arranged at equal intervals on the side of the liquid outlet pipe 43. Specifically, the guide pipe 43a is an inverted L-shaped structure, and the guide pipe 43a is arranged obliquely so that the coolant drips onto the mixing drum 44.

[0043] In practical applications, the protective cover 2 is fixedly connected to the bottom end of the liquid outlet pipe 43 , so that the anti-slip cover 2 moves up and down along with the liquid outlet pipe 43 .

[0044] like Fig.11 As shown, the mixing drum 44 includes a mixing drum body 44a, rotating blades 44b and a stirring rod 44c.

[0045] The mixing drum body 44a is rotatably connected to the lower end of the liquid outlet pipe 43; The rotating blade 44b is arranged on the side of the mixing drum body 44a and is located between the guide tube 43a and the protective cover 2; in actual use, under the action of the piston 41a, the coolant in the liquid storage tube 42 flows out from the guide tube 43c and drips onto the rotating blade 44b, thereby pushing the rotating blade 44b to rotate, achieving stirring of the biological tissue while cooling the biological tissue through the coolant.

[0046] The stirring rod 44d is disposed on the side of the mixing drum body 44a and is slidably connected to the side of the mixing drum body 44a; wherein the stirring rod 44d is located below the rotating blade 44b. Thus, when in use, the stirring rod 44d can stir the biological tissue between the two grinding balls 33a, thereby further quickly stirring the biological tissue uniformly.

[0047] Specifically, a slide groove is provided on the side of the mixing drum body 44a, and an arc-shaped slider is provided at the end of the mixing rod 44d, and the arc-shaped slider is located in the slide groove on the side of the mixing drum body 44a. In this way, during the rotation of the mixing drum body 44a, the mixing rod 44d slides in the slide groove on the side of the mixing drum body 44a. Since biological tissues may be aggregated together due to their own viscosity and other properties, the mixing rod 44d exerts force on the biological tissues from different directions and angles, just like constantly changing the direction of water flow to break up the sediment in the water, making it difficult for the biological tissues to aggregate.

[0048] In actual use, the liquid outlet pipe 43 is sleeved with a spring 8, and the upper end of the spring 8 is connected to the bottom surface of the liquid storage pipe 42, and the lower end of the spring 8 is connected to the upper surface of the push ring 32. Before the push ring 32 is not in contact with the bottom surface of the first circular groove 62a, Figure 6 , 7As shown in FIG. 11 , the spring 8 is in a natural state. At this time, the convex strip 32a on the push ring 32 is at the lower end of the slide groove at the opening of the housing 1, and the grinding ball 33a protective cover 2 is pressed downward by the two grinding balls 33a, and is in an inverted bowl shape; When the housing 1 completely enters the first circular groove 62a, the push ring 32 is subjected to an upward extrusion force from the bottom surface of the first circular groove 62a, so that the push ring 32 drives the hollow shaft motor 31 to enter the opening of the housing 1. At this time, the grinding balls 33a move upward with the push ring 32, no longer press the protective cover 2 downward, and the grinding balls 33a only contact the surface of the protective cover 2. At this time, the protective cover 2 wraps the grinding balls 33a to prevent the grinding balls 33a from damaging the inner wall of the centrifuge tube 7. Fig.14 and 17 As shown, the spring 8 is compressed by the upward extrusion force of the push ring 32. At this time, the convex strip 32a on the push ring 32 is completely located in the slide groove at the opening of the shell 1; when the shell 1 completely enters the stepped groove 62a, the protective cover 2 is overturned by the extrusion force of the bottom surface of the centrifuge tube 7, and switches from the inverted bowl shape to the bowl shape; After the grinding is completed, the lifting rod drives the shell 1 and the grinding mechanism 3 and the low-temperature stirring mechanism 4 arranged in the shell 1 to move upward at the same time. During the upward movement of the grinding mechanism 3, the spring 8 is no longer subjected to the squeezing force of the push ring 32 and gradually returns to its natural state. The spring 8 squeezes the push ring 32 downward until the push ring 32 is in the initial position. At this time, the grinding balls 33a of the two grinding rods 33 move downward synchronously with the push ring 32, that is, while the liquid outlet pipe 43 moves upward, the two grinding balls 33a move downward under the action of the spring 8, pressing downward the two ends of the protective cover 2 located at the bottom end of the liquid outlet pipe 43, so that the protective cover 2 turns downward, and then the protective cover 2 switches from a bowl-shaped shape to an inverted bowl-shaped shape. In this way, when the ground tissue in the centrifuge tube 7 is flushed with liquid, the tissue remaining on the protective cover 2 is flushed into the centrifuge tube to realize the recovery of biological samples.

[0049] The working principle of the grinding device for biological tissue provided by the present invention is: The lifting rod 5 drives the housing 11 to rise so that the medical staff can place the biological tissue in the centrifuge tube 7 so that the biological tissue is located above the anti-slip cover 2; then, the push rod part of the lifting rod 5 retreats to lower the height of the housing 1 until the housing 1 completely enters the first circular groove 62a. At this time, the push ring 32 is subjected to the upward squeezing force of the first circular groove 62a, and the spring 8 on the push ring 32 is compressed, and the grinding ball 33a no longer exerts a downward squeezing force on the protective cover 2; Then, the electric push rod 41 and the centrifugal shaft motor 31 are turned on, and the electric push rod 41 drives the piston 41a to move downward, squeezing the coolant in the liquid storage tube 42 into the liquid outlet tube 43, and then the coolant flows out from the guide tube 43a and drips onto the rotating blade 44b, so that the rotating blade 44b drives the mixing drum body 44a to rotate synchronously, so that the biological tissue is stirred and cooled by the coolant at the same time; and in the process of the mixing drum body 44a rotating, the stirring rod 44c slides in the slide groove on the side of the mixing drum body 44a, so as to stir the biological tissue between the two grinders 33a and break up the biological tissue at the same time; in the process of the rotating blade 44b and the stirring rod 44c stirring and breaking up the biological tissue, the coolant cools down the biological tissue to prevent the protein denaturation of the biological tissue; When the outer shell 1 completely enters the first circular groove 62a, the protective cover 2 is squeezed by the curved bottom surface inside the centrifuge tube 7 and switches from an inverted bowl shape to a bowl shape, thereby protecting the inner wall of the centrifuge tube 7. When the grinding ball 31a rotates to grind the biological tissue, the protective cover 2 can prevent the inner wall of the centrifuge tube 7 from contacting the grinding ball, thereby preventing the inner wall surface of the centrifuge tube 7 from being worn.

[0050] The specific usage process is: Step 1: The height of the housing 1 is raised by the lifting rod 5 so that the operator can place the biological tissue in the centrifuge tube 7. At this time, the biological tissue is located above the inverted bowl-shaped protective cover 2; Step 2: lowering the height of the lifting rod 5, so that the heights of the housing 1, the protective cover 2, the grinding mechanism 3 and the low-temperature stirring mechanism 4 are lowered synchronously, and the protective cover 2 is gradually lowered until it contacts the inner arc bottom surface of the centrifuge tube 7. After the protective cover 2 is squeezed by the inner arc bottom surface of the centrifuge tube 7, the protective cover 2 turns upward, and the protective cover 2 switches from an inverted bowl shape to a bowl shape. At this time, the biological tissue is located above the bowl-shaped protective cover 2 and contacts the grinding balls 33a. At the same time, the housing 1 continues to move downward until the housing 1 completely enters the first circular groove 62a. At this time, the bottom surface of the push ring 32 contacts the bottom surface of the first circular groove 62a, and the convex strip 32a on the outer surface of the push ring 32 completely enters the slide groove at the opening of the housing 1 of the housing 11, that is, the push ring 32 completely enters the opening of the housing 1. At this time, the spring 8 is compressed after being squeezed upward by the push ring 32, and the grinding ball 33a no longer applies downward pressing force to the protective cover 2 and only contacts the surface of the protective cover 2; Step 3: Turn on the electric push rod 41 so that the push rod part of the electric push rod 41 extends. At this time, the piston 41a located at the end of the push rod part of the electric push rod 41 moves downward in the liquid storage tube 42 to squeeze the coolant in the liquid storage tube 42 into the liquid outlet tube 43, and then the coolant flows out from the guide tube 43a and drips onto the rotating blade 44b. While pushing the rotating blade 44b to rotate, the coolant drips from the rotating blade 44b into the centrifuge tube 7 to cool the biological tissue while stirring it to prevent protein denaturation of the biological tissue; When the rotating blade 44b rotates, it drives the mixing drum body 44a to rotate. When the mixing drum body 44a rotates, the stirring rod 44 slides in the slide groove on the side of the mixing drum body 44a. Since biological tissues may aggregate together due to their own viscosity and other characteristics, the stirring rod 44d applies force to the biological tissues from different directions and angles, just like constantly changing the direction of water flow to break up the sediment in the water, making it difficult for the biological tissues to aggregate. Step 4: Turn on the hollow shaft motor 31, the hollow shaft motor 31 drives the two grinding rods 33 to rotate, and then the grinding balls 33a on the lower ends of the grinding rods 33 rotate to grind the biological tissue; After grinding is completed, the height of the lifting rod 5 rises, driving the protective cover 2, the grinding mechanism 3 and the low-temperature stirring mechanism 4 in the shell 1 to move upward synchronously. During the upward movement of the grinding mechanism 3, the spring 8 gradually returns to its natural state. During the gradual return of the spring 8 to its natural state, the spring 8 presses the push ring 32 downward, causing the push ring 32 to move toward the outside of the opening of the shell 1 until the convex strip 32a is located at the lower end of the slide groove at the opening of the shell 1. At this time, the hollow shaft motor 31 drives the two grinding rods 33 to move downward until the grinding balls 33a of the two grinding rods 33 return to the initial position. At this time, the two grinding balls 33a apply a downward pressing force to both ends of the bowl-shaped protective cover 2, causing the bowl-shaped protective cover 2 to flip downward, thereby switching the protective cover 2 from a bowl-shaped form to an inverted bowl-shaped form, so that when the ground tissue in the centrifuge tube is subsequently flushed, the tissue remaining on the protective cover is flushed into the centrifuge tube to achieve the recovery of the biological sample.

[0051] The contents not described in detail in this specification belong to the prior art known to professional and technical personnel in this field.

Claims

1. A grinding device for biological tissue, characterized in that: include: Centrifuge tube base (6); The housing (1) is arranged above the centrifuge tube base (6) and is connected to the centrifuge tube base (6) via a lifting rod (5); A fixed column (61) is arranged on the top of the centrifuge tube base (6), and a centrifuge tube (7) is arranged inside the fixed column (61); A grinding mechanism (3), the upper end of which is slidably connected to the housing (1) and the lower end of which is located inside the centrifuge tube (7); A low-temperature stirring mechanism (4), the upper end of which is located inside the outer shell (1) and connected to the outer shell (1); The protective cover (2) is a hemispherical structure and is connected to the lower end of the low-temperature stirring mechanism (4).

2. A grinding device for biological tissue according to claim 1, characterized in that: The grinding mechanism (3) comprises: Hollow shaft motor (31); A push ring (32) is sleeved on the outer surface of the hollow shaft motor (31), and the inner surface of the push ring (32) is connected to the outer surface of the hollow shaft motor (31), and the outer surface of the push ring (32) is slidably connected to the housing (1); Two grinding rods (33) are located between the push ring (32) and the protective cover (2), and the upper end of each grinding rod (33) is connected to the lower surface of the hollow shaft motor (31), and the lower end is provided with a grinding ball (33a).

3. A grinding device for biological tissue according to claim 2, characterized in that: The low temperature stirring mechanism (4) comprises: An electric push rod (41), wherein a fixed end of the electric push rod (41) is connected to the housing (1), and a piston (41a) is provided at the push rod end of the electric push rod (41); A liquid storage tube (42), the upper end of which is sleeved with the piston (41a), and the lower end of which passes through the hollow shaft motor (31) and is connected to the protective cover (2), and the lower end of the liquid storage tube (42) is located between the two grinding rods (33); A liquid outlet pipe (43), the upper end of which is connected to the liquid storage pipe (42), the lower end of which passes through the hollow shaft of the hollow shaft motor (31) and is connected to the protective cover (2), and a plurality of guide pipes (43a) are provided on the lower end of the liquid outlet pipe (43); The mixing drum (44) is sleeved on the lower end of the liquid outlet pipe (43) and is rotatably connected to the liquid outlet pipe (43).

4. A grinding device for biological tissue according to claim 3, characterized in that: A spring (8) is sleeved on the liquid outlet pipe (43), and the upper end of the spring (8) is connected to the bottom surface of the liquid storage pipe (42), and the lower end is connected to the upper surface of the hollow shaft motor (31).

5. A grinding device for biological tissue according to claim 4, characterized in that: The mixing drum (44) comprises: Mixing drum body (44a); A plurality of rotating blades (44b) are arranged at equal intervals on the side surface of the mixing drum body (44a).

6. A grinding device for biological tissue according to claim 5, characterized in that: The mixing drum body (44a) is also provided with a plurality of stirring rods (44d) for stirring the biological tissue located between the two grinding balls (33a).

7. A grinding device for biological tissue according to claim 1, characterized in that: The fixing column (61) is provided with a stepped groove (62) with an opening facing upward.

8. A grinding device for biological tissue according to claim 7, characterized in that: The stepped groove (62) comprises: A first circular groove (62a) is located on the top surface of the fixing column (61); The second circular groove (62b) is located at the lower end of the first circular groove (62a) and is in communication with the first circular groove (62a); the upper surface of the second circular groove (62b) is in contact with the lower surface of the push ring (32).

9. A grinding device for biological tissue according to claim 2, characterized in that: A convex strip (32a) is provided on the outer surface of the push ring (32), and a sliding groove matching and slidably connected with the convex strip (32a) is provided at the opening of the housing (1).

Images