Microbial agent granule and preparation method thereof

Abstract

The invention discloses microbial agent granules and a preparation method thereof, and belongs to the field of microbial agents, and the granules are prepared from the following raw materials in parts by weight: 5-20 parts of a compound microbial agent, 20-50 parts of fulvic acid, 1-3 parts of essential elements, 30-80 parts of porous silicon spheres, 10-30 parts of organic acid, 1-5 parts of amino acid and 1-10 parts of vitamins. The nitrogen element content of soil can be increased, the microbial agent is suitable for a wide temperature range, the survival rate of effective viable bacteria is high, the soil hardening condition can be improved, the effect on the soil is stable and long-acting, and the adaptability is high.

Description

Technical Field

[0001] The present invention relates to the field of microbial agents, and in particular to a microbial agent granule and a preparation method thereof. Background Art

[0002] Microbial agents refer to the use of active microorganisms or their metabolites to utilize, regulate or treat certain substances. They have become an important research subject in modern agriculture, environmental protection and life sciences. Microbial agents have a wide range of applications, mainly in agriculture, environmental protection and industry. They can promote soil and plant health and increase crop yield and quality.

[0003] The effect of compound microorganisms will be affected by the growth habits of different strains. Blind compounding can easily cause antagonism between strains and affect the effect. Therefore, it is not the case that the more types of compound bacteria, the better. Instead, the best effect can be achieved when the strains can work together and promote each other.

[0004] Nitrogen, phosphorus, potassium and other elements in the soil are important for plant growth. Nitrogen is involved in the formation of proteins and enzymes in crops, which can promote healthy and thick leaves with bright colors, thereby improving photosynthesis efficiency and increasing crop yields and quality. Nitrogen is essential for the growth and development of crops. Lack of nitrogen will lead to slow plant growth, thin and small leaves, yellowing, and even death in severe cases. Soil nitrogen will turn into nitrate nitrogen under hypoxic conditions. Reduced to nitrogen by denitrifying bacteria , resulting in nitrogen loss; nitrogen loss in the form of gas may also occur due to processes such as double decomposition and nitrite decomposition. To this end, the present invention proposes a microbial agent granule and a preparation method thereof. Summary of the invention

[0005] In view of the above-mentioned problems, the present invention proposes a microbial agent granule and a preparation method thereof. The present invention can increase the nitrogen content in the soil. The microbial agent is suitable for a wider temperature range, has a high survival rate of effective live bacteria, has a stable and long-lasting effect on the soil, and has strong adaptability.

[0006] In order to achieve the above-mentioned purpose, the present invention adopts the following technical solutions: A microbial agent granule, the granule is prepared from the following raw materials in parts by weight: 5-20 parts of composite microbial agent, 20-50 parts of fulvic acid, 1-3 parts of essential elements, 30-80 parts of porous silicon balls, 10-30 parts of organic acid, 1-5 parts of amino acid and 1-10 parts of vitamins; Wherein, the composite microbial agent includes nitrogen-fixing bacteria, phosphate bacteria and silicate microbial agent; Wherein, the porous silicon sphere particles have a diameter of 10-80 μm.

[0007] Preferably, the granules are prepared from the following raw materials in parts by weight: 10-15 parts of composite microbial agents, 30-40 parts of fulvic acid, 1-2 parts of essential elements, 45-65 parts of porous silicon balls, 15-25 parts of organic acids, 2-4 parts of amino acids and 3-8 parts of vitamins.

[0008] Preferably, the granules are prepared from the following raw materials in parts by weight: 12 parts of composite microbial agents, 35 parts of fulvic acid, 1.5 parts of essential elements, 50 parts of porous silicon balls, 20 parts of organic acids, 3 parts of amino acids and 5 parts of vitamins.

[0009] Preferably, the nitrogen-fixing bacteria are composed of a mixture of nitrogen-fixing bacteria, rhizobia and nitrogen-fixing cyanobacteria, and the mass ratio of the nitrogen-fixing bacteria, rhizobia and nitrogen-fixing cyanobacteria is 1:2:2.

[0010] Preferably, the phosphate bacteria include Bacillus megaterium and Bacillus megaterium phosphate-solubilizing, and the mass ratio of the Bacillus megaterium to the Bacillus megaterium phosphate-solubilizing is 1:1.

[0011] Preferably, the silicate microbial inoculant includes bridge bacteria and bacillus, and the mass ratio of the bridge bacteria to the bacillus is 1:2.

[0012] Preferably, the essential elements include macroelements and trace elements; the macroelements include nitrogen, phosphorus, potassium, calcium, sulfur, sodium, and magnesium; and the trace elements include iron, boron, manganese, and zinc.

[0013] Preferably, the vitamins include vitamin B1, vitamin B2, vitamin B6 and vitamin B12.

[0014] Preferably, the method for preparing the microbial agent granules comprises the following steps: (1) Add the composite microbial agent, fulvic acid and essential elements into a high-speed blender and mix them, controlling the stirring temperature at 25-28°C; (2) Add the porous silicon balls, control the stirring speed at 300-400 rpm, continue ultrasonication during the stirring process, and mix for 20-40 minutes; (3) Finally, organic acid, amino acid and vitamin are added, mixed evenly and passed through a disc granulator with a rotation speed of 2-2.5 rpm and a particle diameter of 1.5-2 mm. The granules are obtained.

[0015] Due to the adoption of the above technical scheme, the beneficial effects of the present invention are as follows: the present invention can convert nitrogen in the air into ammonia that can be used by plants, has a good nitrogen fixation effect, and increases the nitrogen content in the soil; the microbial granules of the present invention have a higher survival rate of effective live bacteria, are suitable for a wider temperature range, and have a high survival rate; improve soil compaction, have a stable and long-lasting effect on the soil, and have strong adaptability. DETAILED DESCRIPTION

[0016] To make the purpose, technical solution and advantages of the embodiments of the present invention clearer, the technical solution in the embodiments of the present invention will be clearly and completely described below in combination with the embodiments of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention. Embodiment 1:

[0017] The preparation method of the microbial agent granules of this embodiment 1 specifically comprises the following steps: (1) Weigh the following raw materials by weight: 12 parts of composite microbial agent, 35 parts of fulvic acid, 1.5 parts of essential elements, 50 parts of porous silicon balls, 20 parts of organic acid, 3 parts of amino acid and 5 parts of vitamins; Among them, the composite microbial agent includes nitrogen-fixing bacteria, phosphate bacteria and silicate microbial agent; Among them, the diameter of the porous silicon sphere particles is 15μm.

[0018] Among them, the nitrogen-fixing bacteria are composed of a mixture of nitrogen-fixing bacteria, rhizobia, and nitrogen-fixing cyanobacteria, and the mass ratio of nitrogen-fixing bacteria, rhizobia, and nitrogen-fixing cyanobacteria is 1:2:2.

[0019] Among them, the phosphate bacteria include Bacillus megaterium and Bacillus megaterium phosphate-solubilizing, and the mass ratio of Bacillus megaterium to Bacillus megaterium phosphate-solubilizing is 1:1.

[0020] Among them, the silicate microbial inoculants include bridge bacteria and bacillus, and the mass ratio of bridge bacteria to bacillus is 1:2.

[0021] Among them, essential elements include macroelements and trace elements; macroelements include nitrogen, phosphorus, potassium, calcium, sulfur, sodium, and magnesium; trace elements include iron, boron, manganese, and zinc.

[0022] Among them, vitamins include vitamin B1, vitamin B2, vitamin B6 and vitamin B12.

[0023] (2) After weighing the raw materials, add the composite microbial agent, fulvic acid and essential elements into a high-speed stirrer and mix and stir, controlling the stirring temperature at 25°C; (3) Add the porous silicon spheres, control the stirring speed at 350 rpm, continue ultrasonication during the stirring process, and mix for 30 min; (4) Finally, organic acid, amino acid and vitamin are added, mixed evenly and passed through a disc granulator with the speed controlled at 2.0 rpm and the particle diameter at 2.0 mm. Granules are obtained after discharge. Embodiment 2:

[0024] The preparation method of the microbial agent granules of this embodiment 2 specifically comprises the following steps: (1) Weigh the following raw materials by weight: 20 parts of composite microbial agent, 50 parts of fulvic acid, 3 parts of essential elements, 80 parts of porous silicon balls, 30 parts of organic acid, 5 parts of amino acid and 10 parts of vitamins; Among them, the composite microbial agent includes nitrogen-fixing bacteria, phosphate bacteria and silicate microbial agent; Among them, the diameter of the porous silicon sphere particles is 20μm.

[0025] Among them, the nitrogen-fixing bacteria are composed of a mixture of nitrogen-fixing bacteria, rhizobia, and nitrogen-fixing cyanobacteria, and the mass ratio of nitrogen-fixing bacteria, rhizobia, and nitrogen-fixing cyanobacteria is 1:2:2.

[0026] Among them, the phosphate bacteria include Bacillus megaterium and Bacillus megaterium phosphate-solubilizing, and the mass ratio of Bacillus megaterium to Bacillus megaterium phosphate-solubilizing is 1:1.

[0027] Among them, the silicate microbial inoculants include bridge bacteria and bacillus, and the mass ratio of bridge bacteria to bacillus is 1:2.

[0028] Among them, essential elements include macroelements and trace elements; macroelements include nitrogen, phosphorus, potassium, calcium, sulfur, sodium, and magnesium; trace elements include iron, boron, manganese, and zinc.

[0029] Among them, vitamins include vitamin B1, vitamin B2, vitamin B6 and vitamin B12.

[0030] (2) After weighing the raw materials, add the composite microbial agent, fulvic acid and essential elements into a high-speed stirrer and mix and stir, controlling the stirring temperature at 26°C; (3) Add the porous silicon balls, control the stirring speed at 400 rpm, continue ultrasonication during the stirring process, and mix for 40 min; (4) Finally, organic acid, amino acid and vitamin are added, mixed evenly and passed through a disc granulator with the speed controlled at 2.5 rpm and the particle diameter at 2.0 mm. Granules are obtained. Embodiment 3:

[0031] The preparation method of the microbial agent granules of this embodiment 3 specifically includes the following steps: (1) Weigh the following raw materials by weight: 5 parts of composite microbial agent, 20 parts of fulvic acid, 1 part of essential elements, 30 parts of porous silicon balls, 10 parts of organic acid, 1 part of amino acid and 1 part of vitamin; Among them, the composite microbial agent includes nitrogen-fixing bacteria, phosphate bacteria and silicate microbial agent; Among them, the diameter of the porous silicon sphere particles is 25μm.

[0032] Among them, the nitrogen-fixing bacteria are composed of a mixture of nitrogen-fixing bacteria, rhizobia, and nitrogen-fixing cyanobacteria, and the mass ratio of nitrogen-fixing bacteria, rhizobia, and nitrogen-fixing cyanobacteria is 1:2:2.

[0033] Among them, the phosphate bacteria include Bacillus megaterium and Bacillus megaterium phosphate-solubilizing, and the mass ratio of Bacillus megaterium to Bacillus megaterium phosphate-solubilizing is 1:1.

[0034] Among them, the silicate microbial inoculants include bridge bacteria and bacillus, and the mass ratio of bridge bacteria to bacillus is 1:2.

[0035] Among them, essential elements include macroelements and trace elements; macroelements include nitrogen, phosphorus, potassium, calcium, sulfur, sodium, and magnesium; trace elements include iron, boron, manganese, and zinc.

[0036] Among them, vitamins include vitamin B1, vitamin B2, vitamin B6 and vitamin B12.

[0037] (2) After weighing the raw materials, add the composite microbial agent, fulvic acid and essential elements into a high-speed stirrer and mix and stir, controlling the stirring temperature at 27°C; (3) Add the porous silicon spheres, control the stirring speed at 300 rpm, continue ultrasonication during the stirring process, and mix for 35 min; (4) Finally, organic acid, amino acid and vitamin are added, mixed evenly and passed through a disc granulator with the speed controlled at 2.5 rpm and the particle diameter at 2.0 mm. Granules are obtained. Embodiment 4:

[0038] The preparation method of the microbial agent granules of this embodiment 4 specifically comprises the following steps: (1) Weigh the following raw materials by weight: 15 parts of composite microbial agent, 40 parts of fulvic acid, 2 parts of essential elements, 65 parts of porous silicon balls, 25 parts of organic acid, 4 parts of amino acid and 8 parts of vitamins; Among them, the composite microbial agent includes nitrogen-fixing bacteria, phosphate bacteria and silicate microbial agent; Among them, the diameter of the porous silicon sphere particles is 30μm.

[0039] Among them, the nitrogen-fixing bacteria are composed of a mixture of nitrogen-fixing bacteria, rhizobia, and nitrogen-fixing cyanobacteria, and the mass ratio of nitrogen-fixing bacteria, rhizobia, and nitrogen-fixing cyanobacteria is 1:2:2.

[0040] Among them, the phosphate bacteria include Bacillus megaterium and Bacillus megaterium phosphate-solubilizing, and the mass ratio of Bacillus megaterium to Bacillus megaterium phosphate-solubilizing is 1:1.

[0041] Among them, the silicate microbial inoculants include bridge bacteria and bacillus, and the mass ratio of bridge bacteria to bacillus is 1:2.

[0042] Among them, essential elements include macroelements and trace elements; macroelements include nitrogen, phosphorus, potassium, calcium, sulfur, sodium, and magnesium; trace elements include iron, boron, manganese, and zinc.

[0043] Among them, vitamins include vitamin B1, vitamin B2, vitamin B6 and vitamin B12.

[0044] (2) After weighing the raw materials, add the composite microbial agent, fulvic acid and essential elements into a high-speed stirrer and mix and stir, controlling the stirring temperature at 28°C; (3) Add the porous silicon balls, control the stirring speed at 400 rpm, continue ultrasonication during the stirring process, and mix for 40 min; (4) Finally, organic acid, amino acid and vitamin are added, mixed evenly and passed through a disc granulator with the speed controlled at 2.5 rpm and the particle diameter at 1.5 mm. Granules are obtained. Embodiment 5:

[0045] The preparation method of the microbial agent granules of this embodiment 5 specifically comprises the following steps: (1) Weigh the following raw materials by weight: 10 parts of composite microbial agent, 30 parts of fulvic acid, 1 part of essential elements, 45 parts of porous silicon balls, 15 parts of organic acids, 2 parts of amino acids and 3 parts of vitamins; Among them, the composite microbial agent includes nitrogen-fixing bacteria, phosphate bacteria and silicate microbial agent; Among them, the diameter of the porous silicon sphere particles is 60μm.

[0046] Among them, the nitrogen-fixing bacteria are composed of a mixture of nitrogen-fixing bacteria, rhizobia, and nitrogen-fixing cyanobacteria, and the mass ratio of nitrogen-fixing bacteria, rhizobia, and nitrogen-fixing cyanobacteria is 1:2:2.

[0047] Among them, the phosphate bacteria include Bacillus megaterium and Bacillus megaterium phosphate-solubilizing, and the mass ratio of Bacillus megaterium to Bacillus megaterium phosphate-solubilizing is 1:1.

[0048] Among them, the silicate microbial inoculants include bridge bacteria and bacillus, and the mass ratio of bridge bacteria to bacillus is 1:2.

[0049] Among them, essential elements include macroelements and trace elements; macroelements include nitrogen, phosphorus, potassium, calcium, sulfur, sodium, and magnesium; trace elements include iron, boron, manganese, and zinc.

[0050] Among them, vitamins include vitamin B1, vitamin B2, vitamin B6 and vitamin B12.

[0051] (2) After weighing the raw materials, add the composite microbial agent, fulvic acid and essential elements into a high-speed stirrer and mix and stir, controlling the stirring temperature at 25°C; (3) Add the porous silicon spheres, control the stirring speed at 300 rpm, continue ultrasonication during the stirring process, and mix for 35 min; (4) Finally, organic acid, amino acid and vitamin are added, mixed evenly and passed through a disc granulator with the speed controlled at 2.5 rpm and the particle diameter at 1.5 mm. Granules are obtained.

[0052] Comparative Example 1: The preparation method of the microbial agent granules in this comparative example 1 specifically comprises the following steps: (1) Weigh the following raw materials by weight: 35 parts of fulvic acid, 1.5 parts of essential elements, 50 parts of porous silicon balls, 20 parts of organic acids, 3 parts of amino acids and 5 parts of vitamins; Among them, the diameter of the porous silicon sphere particles is 15μm.

[0053] Among them, essential elements include macroelements and trace elements; macroelements include nitrogen, phosphorus, potassium, calcium, sulfur, sodium, and magnesium; trace elements include iron, boron, manganese, and zinc.

[0054] Among them, vitamins include vitamin B1, vitamin B2, vitamin B6 and vitamin B12.

[0055] (2) After weighing the raw materials, add fulvic acid and essential elements into a high-speed stirrer and mix them, controlling the stirring temperature at 25°C; (3) Add the porous silicon spheres, control the stirring speed at 350 rpm, continue ultrasonication during the stirring process, and mix for 30 min; (4) Finally, organic acid, amino acid and vitamin are added, mixed evenly and passed through a disc granulator with the speed controlled at 2.0 rpm and the particle diameter at 2.0 mm. Granules are obtained after discharge.

[0056] Comparative Example 2: The preparation method of the microbial agent granules in this comparative example 2 specifically comprises the following steps: (1) Weigh the following raw materials by weight: 12 parts of composite microbial agent, 35 parts of fulvic acid, 1.5 parts of essential elements, 20 parts of organic acid, 3 parts of amino acid and 5 parts of vitamins; Among them, the composite microbial agent includes nitrogen-fixing bacteria, phosphate bacteria and silicate microbial agent; Among them, the nitrogen-fixing bacteria are composed of a mixture of nitrogen-fixing bacteria, rhizobia, and nitrogen-fixing cyanobacteria, and the mass ratio of nitrogen-fixing bacteria, rhizobia, and nitrogen-fixing cyanobacteria is 1:2:2.

[0057] Among them, the phosphate bacteria include Bacillus megaterium and Bacillus megaterium phosphate-solubilizing, and the mass ratio of Bacillus megaterium to Bacillus megaterium phosphate-solubilizing is 1:1.

[0058] Among them, the silicate microbial inoculants include bridge bacteria and bacillus, and the mass ratio of bridge bacteria to bacillus is 1:2.

[0059] Among them, essential elements include macroelements and trace elements; macroelements include nitrogen, phosphorus, potassium, calcium, sulfur, sodium, and magnesium; trace elements include iron, boron, manganese, and zinc.

[0060] Among them, vitamins include vitamin B1, vitamin B2, vitamin B6 and vitamin B12.

[0061] (2) After weighing the raw materials, add the composite microbial agent, fulvic acid and essential elements into a high-speed stirrer and mix and stir, controlling the stirring temperature at 25°C; (3) Continuous ultrasound during stirring for 30 minutes; (4) Finally, organic acid, amino acid and vitamin are added, mixed evenly and passed through a disc granulator with the speed controlled at 2.0 rpm and the particle diameter at 2.0 mm. Granules are obtained after discharge.

[0062] 1. Detect the content of N, K and P elements in the soil after the microbial agent granules are applied at different times and temperatures; The granules prepared in this example and the comparative example were tested for element content. The granules were mixed in soil, and the element content in the soil was measured at different times. The nitrogen determination was performed according to the Kjeldahl method and the statistical data were obtained.

[0063] The element content in the soil was measured at different temperatures (0℃, 25℃, 45℃) and different time periods (3h and 12hmin); the content of N, K, and P in the soil in the initial state was 0.06%, K: 1.23%, and P 0.08%, respectively. After the test, the data was statistically recorded and drawn into a table.

[0064]

[0065]

[0066] Based on the above Table 1-2, we can conclude that: (1) It can be concluded from Examples 1-5 and Comparative Examples 1-2 that the microbial agent granules prepared in this example have a good nitrogen fixation effect, can convert nitrogen in the air into ammonia that can be used by plants, increase the nitrogen content in the soil, and provide more nutrients for crops; (2) Porous silicon balls facilitate the attachment of composite microbial agents, which can improve soil compaction, increase the soil spatial structure, facilitate the interaction between microbial agents and soil, and further enhance the role of microorganisms.

[0067] 2. Detect the survival rate of effective live bacteria in microbial inoculant granules at different temperatures and times: The granules were placed in a drying drum at temperatures of 15, 20, 25, 30, 35, 40, and 45°C and for 1, 6, 12, and 36 hours, and the survival rate of the effective live bacteria in the granules was determined.

[0068]

[0069]

[0070]

[0071]

[0072] Based on the above Tables 1-4, we can conclude that: (1) The microbial granules prepared in this embodiment have a high survival rate of effective live bacteria, and the microbial granules are suitable for a wide temperature range and have a high survival rate; (2) The microbial granules prepared in this example have a stable and long-lasting effect on the soil and have strong adaptability.

[0073] The above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit the same. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that the technical solutions described in the aforementioned embodiments may still be modified, or some of the technical features may be replaced by equivalents. However, these modifications or replacements do not deviate the essence of the corresponding technical solutions from the spirit and scope of the technical solutions of the embodiments of the present invention.

Claims

1. A microbial agent granule, characterized in that: The granules are prepared from the following raw materials in parts by weight: 5-20 parts of composite microbial agent, 20-50 parts of fulvic acid, 1-3 parts of essential elements, 30-80 parts of porous silicon balls, 10-30 parts of organic acid, 1-5 parts of amino acid and 1-10 parts of vitamins; Wherein, the composite microbial agent includes nitrogen-fixing bacteria, phosphate bacteria and silicate microbial agent; Wherein, the porous silicon sphere particles have a diameter of 10-80 μm.

2. The microbial agent granules according to claim 1, characterized in that: The granules are prepared from the following raw materials in parts by weight: 10-15 parts of composite microbial agents, 30-40 parts of fulvic acid, 1-2 parts of essential elements, 45-65 parts of porous silicon balls, 15-25 parts of organic acids, 2-4 parts of amino acids and 3-8 parts of vitamins.

3. The microbial agent granules according to claim 2, characterized in that: The granules are prepared from the following raw materials in parts by weight: 12 parts of composite microbial agents, 35 parts of fulvic acid, 1.5 parts of essential elements, 50 parts of porous silicon balls, 20 parts of organic acids, 3 parts of amino acids and 5 parts of vitamins.

4. The microbial agent granules according to claim 1, characterized in that: The nitrogen-fixing bacteria are composed of a mixture of nitrogen-fixing bacteria, rhizobia and nitrogen-fixing cyanobacteria, and the mass ratio of the nitrogen-fixing bacteria, rhizobia and nitrogen-fixing cyanobacteria is 1:2:

2.

5. The microbial agent granules according to claim 1, characterized in that: The phosphate bacteria include Bacillus megaterium and Bacillus megaterium phosphate-solubilizing, and the mass ratio of the Bacillus megaterium to the Bacillus megaterium phosphate-solubilizing is 1:

1.

6. The microbial agent granules according to claim 1, characterized in that: The silicate microbial inoculant includes bridge bacteria and bacillus, and the mass ratio of the bridge bacteria to the bacillus is 1:

2.

7. The microbial agent granules according to claim 1, characterized in that: The essential elements include macroelements and trace elements; the macroelements include nitrogen, phosphorus, potassium, calcium, sulfur, sodium, and magnesium; the trace elements include iron, boron, manganese, and zinc.

8. The microbial agent granules according to claim 1, characterized in that: The vitamins include vitamin B1, vitamin B2, vitamin B6 and vitamin B12.

9. The method for preparing the microbial agent granules according to any one of claims 1 to 8, characterized in that: The preparation method of the microbial agent granules specifically comprises the following steps: (1) Add the composite microbial agent, fulvic acid and essential elements into a high-speed blender and mix them, controlling the stirring temperature at 25-28°C; (2) Add the porous silicon balls, control the stirring speed at 300-400 rpm, continue ultrasonication during the stirring process, and mix for 20-40 minutes; (3) Finally, organic acid, amino acid and vitamin are added, mixed evenly and passed through a disc granulator with a rotation speed of 2-2.5 rpm and a particle diameter of 1.5-2 mm. The granules are obtained.

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