Incubator

Abstract

The purpose of the present invention is to provide an incubator capable of generating pressure fluctuations. The incubator comprises an incubator body and a voltage transformation device, the incubator body is provided with a voltage transformation interface, the voltage transformation device is connected with the incubator body through the voltage transformation interface, and the voltage transformation device is used for inputting fluctuating or constant air pressure into the incubator body.

Description

[0001] This invention patent application is a divisional application of the invention patent application with international application number PCT / CN2021 / 106395, international application date 2021.7.15, application number 202180045181.8 entering the Chinese national phase, and name “Incubator”. Technical Field

[0002] The present invention relates to an incubator. Background Art

[0003] The incubator is a box device mainly used to culture microorganisms, plants and animal cells. It simulates the growth environment of microorganisms, tissues, cells, etc., provides stable temperature, humidity and gas concentration, and is widely used in cell, tissue culture and the reproduction and cultivation of some special microorganisms. The principle of the three-gas incubator is the same as other incubators, and its characteristics are that it can not only add CO 2 , nitrogen and oxygen can also be added, and the content of various gases can be controlled and adjusted.

[0004] Most of the incubators do not have a pressure higher than the atmospheric pressure and are mostly set to a constant pressure. The invention patent application with the publication number "CN102978112A" discloses a high-pressure cell culture incubator, which, under a high-pressure environment, in order to maintain the constant pressure required for cell culture in the incubator and to avoid excessive gas pressure in the incubator caused by too fast gas inflow using ordinary valves, uses needle valves for the first valve and the second valve, and the gas in the incubator passing through the first valve and the atmosphere passing through the second valve sequentially pass through an oxygen pressure reducer and a flow meter to enter the gas analyzer to control the amount of sampled gas.

[0005] However, the inventors have found that not only does applying an additional constant pressure in the incubator, especially a three-gas incubator, have a good culture effect; but also when the gas pressure applied in the incubator changes sinusoidally over time, the environment for culturing stem cells is closer to the environment in the human body, and has a better culture effect. Summary of the invention

[0006] The object of the present invention is to provide an incubator which can generate pressure fluctuations.

[0007] According to an embodiment of the present invention, the incubator includes a box body and a voltage transformer. The box body has a voltage transformer interface. The voltage transformer is connected to the box body via the voltage transformer interface. The voltage transformer is used to input fluctuating or constant air pressure into the box body.

[0008] According to an embodiment of the present invention, the pressure-changing device comprises a cylinder and a piston, the cylinder is connected to the box via the pressure-changing interface, and the piston is movably arranged in the cylinder to facilitate input of the gas pressure through movement of the piston.

[0009] According to an embodiment of the present invention, the cylinder body is connected to the pressure changing interface directly or through a pipeline.

[0010] According to an embodiment of the present invention, the cylinder body is directly connected to the pressure-changing interface, and the pressure-changing interface is large enough to enable the cylinder body and the box body to change the air pressure synchronously with the movement of the piston.

[0011] According to an embodiment of the present invention, the cylinder body is fixedly connected to the box body; preferably, the cylinder body is fixedly connected to the top of the box body.

[0012] According to an embodiment of the present invention, the voltage transformation device further comprises one of an electric cylinder, a gas cylinder, and a hydraulic cylinder;

[0013] Preferably, the electric cylinder comprises a servo motor and a transmission mechanism, wherein the transmission mechanism connects the servo motor and the piston to convert the rotation of the servo motor into the movement of the piston;

[0014] Preferably, the electric cylinder is an electric push rod, and the electric push rod can drive the piston to move.

[0015] According to an embodiment of the present invention, the voltage conversion device comprises a gas storage tank, which is connected to the box body through a pressurizing gas path and a decompressing gas path;

[0016] The boosting gas circuit is provided with a boosting pump and a boosting control valve, the boosting pump is used to transport the gas in the gas storage tank to the box body, and the boosting control valve is used to control the on-off of the boosting gas circuit;

[0017] The decompression gas circuit is provided with a decompression pump and a decompression control valve, the decompression pump is used to transport the gas in the box to the gas storage tank, and the decompression control valve is used to control the on-off of the decompression gas circuit.

[0018] According to an embodiment of the present invention, the box body is further provided with a safety valve, and the safety valve opens after the pressure in the box body exceeds a safety threshold.

[0019] According to an embodiment of the present invention, the box body further includes an openable and closable air inlet and an openable and closable air outlet that are independent of the voltage transformation interface.

[0020] According to an embodiment of the present invention, a sterilizing device and / or a humidifying device and / or an electric heating device are provided in the box.

[0021] According to an embodiment of the present invention, the sterilization device is an ultraviolet light emitter or a steam sterilization device.

[0022] According to an embodiment of the present invention, the gas in the incubator is one or more gases; preferably a three-gas incubator; more preferably, the gas is carbon dioxide, nitrogen, and oxygen.

[0023] According to an embodiment of the present invention, the incubator further comprises a gas supply system, wherein a pressure relief device is used to release the gas in the box; and a gas supply system is used to provide gas with stable process parameters to the box while the pressure relief device is releasing the gas.

[0024] According to an embodiment of the present invention, a gas mixing device is provided in the box body, which is used to make the gas in the box body in a turbulent state so as to maintain the uniformity of the gas.

[0025] According to an embodiment of the present invention, the gas supply system includes a premixing tank, which receives one or more gases through an input pipeline, and the premixing tank is connected to the box through an output pipeline. A gas mixing device is provided in the premixing tank, the input pipeline is provided with a gas source control device for the gas source, and the output pipeline is provided with an air intake control device for the box.

[0026] According to an embodiment of the present invention, a gas control device is further provided in the premixing tank, and the gas control device includes a gas concentration detector, a heating device, a humidifying device and / or a temperature and humidity detection device.

[0027] According to an embodiment of the present invention, the gas source control device and / or the air intake control device includes a heating device, a humidifying device and / or a gas filtering device.

[0028] According to the above scheme, the incubator can provide additional applied pressure fluctuations, and thus can be used to simulate the pressure environment in which cells are located, thereby helping to improve the biological activity of the cells. In addition, providing a constant pressure environment also has a good culture effect. BRIEF DESCRIPTION OF THE DRAWINGS

[0029] The above and other features, properties and advantages of the present invention will become more apparent through the following description in conjunction with the accompanying drawings and embodiments, in which:

[0030] Figure 1 It is a cross-sectional view of the incubator body.

[0031] Figure 2 is a schematic diagram of a first embodiment of an incubator.

[0032] Figure 3 is a schematic diagram of a second embodiment of an incubator.

[0033] Figure 4 is a schematic diagram of a third embodiment of an incubator.

[0034] Figure 5 This is an application example of an incubator showing the expansion multiples of hair follicle mesenchymal stem cells cultured under different conditions.

[0035] Figure 6 The figure shows the generation number and expansion multiple of hair follicle mesenchymal stem cells under different culture conditions in an application example of an incubator, wherein the generation number is represented by Pn, and n is a natural number.

[0036] Figure 7 is a schematic diagram of a fourth embodiment of an incubator. DETAILED DESCRIPTION

[0037] like Figure 1 and Figure 2 As shown, the incubator includes a box 16 and a transformer 9. The box 16 has a similar configuration to the existing incubator, providing a space and environment for culturing cells. The following embodiments are described by taking a three-gas incubator as an example, but are not limited thereto.

[0038] like Figure 1 As shown, the box 16 includes a cavity 3, and the box 16 also includes an air inlet 4 located at the top and an exhaust port 5 located at the bottom. A bracket 8 is arranged in the cavity 3, and the bracket 8 is used to place tools such as microbial culture dishes. Figure 2 As shown in the figure, the pressure-changing device 9 includes a cylinder 90 and a piston 91. The cylinder 90 is externally connected to the box 16. The box 16 has a pressure-changing interface not shown in the figure. Through the pressure-changing interface, the space in the cylinder 90 is connected to the cavity 3, so by moving the piston 91, the gas in the cylinder 90 can be compressed, thereby inputting air pressure to the cavity 3. The input air pressure can be an input positive pressure or a negative pressure. Figure 2 As shown, when the piston 91 moves to the left, positive pressure is input, and when it moves to the right, negative pressure is input. The pressure-changing interface can be set to be almost the same size as the cross-section of the cylinder 90, so that the space in the cylinder 90 and the cavity 3 is almost the same space, and the internal air pressure can change synchronously with the movement of the piston 91. In another embodiment, the pressure-changing interface is set to be smaller, and there is a delay in the change of air pressure between the cylinder 90 and the cavity 3. The air pressure inside the two is equal only after the piston 91 stops moving for a period of time.

[0039] Figure 3 Another embodiment is shown, with Figure 1 and Figure 2 Compared with the embodiment shown in the figure, the difference is that the transformer interface is connected to the transformer device 9 through the pipeline 13. In this way, the transformer device 9 and the box 16 can be arranged at a distance, and the transformer device 9 and the box 16 are more flexible in spatial arrangement. Instead of making the transformer device 9 and the box 16 into one piece, the transformer device 9 and the box 16 can be manufactured by different industrial divisions, which can save costs or facilitate the productization of the device.

[0040] In the aforementioned embodiment, the movement of the piston 91 is achieved by connecting the drive device 92. One embodiment of the drive device 92 is an electric cylinder (including a servo motor and a transmission mechanism). The servo motor outputs a rotation with a variable speed, and the transmission mechanism converts the rotation of the servo motor into the movement of the piston 91. The transmission mechanism is, for example, a gear rack or a screw nut transmission mechanism. In a preferred embodiment, the drive device 92 is an electric push rod. In another embodiment, the drive device 92 is a cylinder or a hydraulic cylinder. Through the reciprocating motion of the piston 91, the gas in the box 16 generates fluctuating pressure, and the change of the pressure can be set as required, for example, a sinusoidal change. When the piston 91 moves to a certain position and remains stationary, a constant pressure can be generated in the box. There is a safety valve not shown in the figure on the box 16. When the pressure exceeds a value, the safety valve opens and automatically releases the pressure. The safety valve is also suitable for the embodiments described below.

[0041] Figure 4 Another embodiment is shown. Figure 1 and Figure 2 Compared with the embodiment shown in the figure, the difference is that the pressure conversion device 9 includes a gas storage tank 96, a pressurized gas circuit 94 and a decompressed gas circuit 95. The gas storage tank 96 is connected to the box 16 through the pressurized gas circuit 94 and the decompressed gas circuit 95. The pressurized gas circuit 94 is provided with a booster pump 14 and a booster control valve 17. The booster pump 14 is used to transport the gas in the gas storage tank 96 to the box 16, and the booster control valve 14 is used to control the on and off of the pressurized gas circuit.

[0042] The decompression gas circuit 95 is provided with a decompression pump 15 and a decompression control valve 18. The decompression pump 15 is used to transport the gas in the box 16 to the gas storage tank 96, and the decompression control valve 18 is used to control the opening and closing of the decompression gas circuit.

[0043] When the pressure in the cavity 3 needs to be increased, the control valve 17 in front of the boost pump 14 is opened, and the boost pump 14 starts to draw gas from the gas tank 96 into the cavity 3, while the control valve 18 in front of the pressure reducing pump 15 is closed, and the pressure in the cavity 3 is increased to a pressure value, and the pressure increase is completed; then, the control valve 17 in front of the boost pump 14 is closed, and the control valve 18 in front of the pressure reducing pump 15 is opened, and the pressure reducing pump 15 draws gas in the cavity 3 into the gas tank 96, and the cavity 3 is depressurized to a specified value, and the pressure reduction is completed. The boost pump 14, the pressure reducing pump 15, and the two control valves 17 and 18 can be connected to a controller, and the operation control of the pressure increase and pressure reduction is performed by a program in the controller, and the pressure in the cavity 3 fluctuates periodically over time.

[0044] More detailed settings can also be implemented on the box 16. Figure 1As shown, the shell 1 of the box body 16 of the incubator is a stainless steel tank, which can be a cylinder or a cuboid, with an arc transition on the inner wall. The lid of the stainless steel tank is integrated with the door, and can withstand a certain positive pressure after closing. There is a humidifying device 10 at the bottom of the cavity 3, and a sensor or tester 12 is arranged around it, which is connected to a display not shown in the figure set outside. The sterilizing device 6 can be an ultraviolet light emitter, arranged around the cavity 3, or it can be steam sterilized. The heating device 7 is an electric heater, distributed around the cavity 3. The culture environment of the cells is purified by the sterilizing device 6, and a more realistic culture environment is simulated by the heating device and the humidifying device, and the sensor or tester 12 can be used to feedback information such as temperature, humidity and pressure, and the feedback information is fed to the controller, and the controller compares the feedback signal with the set value to control and adjust the heating device 7, the humidifying device 10, etc.

[0045] An example of application of an incubator is described below.

[0046] "Different conditions" or "different culture conditions" means that the cell culture conditions involved in the comparison differ from each other only in the unique gas environment characteristics indicated below. Such a set of different culture conditions for comparison is referred to as "4 conditions":

[0047] Normoxia (20%): oxygen concentration is 20%, and no additional pressure is applied beyond one atmosphere;

[0048] Hypoxia (5%): oxygen concentration is 5%, and no additional pressure is applied beyond one atmosphere;

[0049] Hypoxic static pressure (5%+static): oxygen concentration is 5%, and a constant pressure of 95 mmHg is additionally applied under 1 atmosphere. The box 16 is pressurized through the pressure transformer 9. When the additional pressure reaches 95 mmHg, the inlet and outlet of the box 16 are dynamically balanced without stopping the inflation and pressurization, and the gas in the box 16 is maintained at 1 atmosphere + 95 mmHg.

[0050] Hypoxic dynamic pressure (5%+dynamic): oxygen concentration is 5%, additional pressure is applied at 1 atmosphere, and the pressure in the box 16 performs sinusoidal periodic fluctuations within the range of 1 atmosphere + 75-115 mmHg, with a frequency of 14 times / minute.

[0051] Example: Comparison of expansion multiples of hair follicle mesenchymal stem cells cultured under different conditions

[0052] 1) Obtain intact human hair follicle tissue and carefully place the hair follicle tissue at the bottom of a 1.5 mL EP tube using microtweezers. Add 5 μL of enzymatic hydrolysis solution TripLE (Gibco-12604021) to each hair follicle and incubate at 37°C with 5% CO 2Incubate in the incubator for 3 hours, carefully flicking the bottom of the tube every hour to mix gently.

[0053] 2) After 3 hours of enzymatic hydrolysis, the outer root sheath layer of the hair follicles can be seen under the microscope to be completely enzymatically hydrolyzed, but the hair shaft cannot be completely enzymatically hydrolyzed. The unenzymatically hydrolyzed part does not need to be removed. Use a 100-1000μL pipette to gently blow 10 times to completely mix the enzymatic solution. Let it stand for 1 minute until the unenzymatically hydrolyzed hair shaft sinks to the bottom of the EP tube, and aspirate the upper enzymatic suspension, which is the primary mesenchymal stem cells.

[0054] 25 μL of primary mesenchymal stem cell suspension obtained by enzymatic hydrolysis of 5 hair follicles was added to one well of a six-well plate, and then 2 mL of amniotic fluid culture medium (purchased from Guangzhou Baiyunshan Baidi Biomedicine Co., Ltd.) was added for re-suspending. The six-well plate was placed in normoxia (20%), hypoxia (5%), hypoxia static pressure (5% + static), and hypoxia dynamic pressure (5% + dynamic) at 37°C, and the medium was changed every 3 days.

[0055] 3) After culturing for 10-12 days, the culture medium can be discarded when the cell density in the six-well plate reaches more than 80%. After adding 0.5 mL of TryplE digestion solution to the bottom of the six-well plate, place it in a 37°C incubator for digestion for 3 minutes, and then add 1 mL of amniotic fluid culture medium to the six-well plate to terminate the digestion. The supernatant is taken out and placed in a 15 mL centrifuge tube. The plate is rinsed once with 1 mL of amniotic fluid culture medium, and the rinse solution is added to the centrifuge tube. Centrifuge at 1500 rpm for 5 minutes in a centrifuge, the supernatant is discarded, 1 mL of amniotic fluid culture medium is added to resuspend, and the counted cells are inoculated into a T25 culture bottle to obtain P1 hair follicle mesenchymal stem cells.

[0056] 4) The cells were continuously subcultured to P12, and the cells under the four conditions always maintained their respective culture conditions. Expansion multiple = number of cells harvested at each subculture / number of cells inoculated. In this example, 5000 cells / cm 2 The area of ​​a T25 culture flask is 25 cm 2 , that is, one T25 culture flask is inoculated with 1.25×10 5 cells, the expansion factor calculation formula is "expansion factor = number of cells harvested each time / (1.25 × 10 5 )”.

[0057] Results Figure 5 , Figure 6 The results showed that the proliferation multiple of mesenchymal stem cells cultured in hypoxia was higher than that in normoxia, and pressurization further increased the proliferation multiple. Under hypoxic dynamic pressure conditions, the proliferation multiple of mesenchymal stem cells was the highest.

[0058] Figure 7Another embodiment of the incubator is shown. The incubator includes a box body 16, a transformer 9 and a gas premixing tank 21. This embodiment uses the component numbers and some contents of the previous embodiment, wherein the same numbers are used to represent the same or similar components, and the description of the same technical contents is selectively omitted. The description of the omitted parts can be referred to the previous embodiment, and this embodiment will not be repeated. Figure 2 , Figure 3 or Figure 4 Compared with the embodiment shown in the figure, the difference of this embodiment is that the cylinder 90 of the transformer 9 is fixedly connected to the top of the box 16, and one of the ways of fixed connection is welding, so that the overall structure is more compact, firm and reliable, and can withstand higher air pressure and faster frequency air pressure fluctuations. There is a transformer interface not shown in the figure between the box 16 and the transformer 9. Through the transformer interface, the space in the cylinder 90 is connected to the box 16, so by moving the piston 91, the gas in the cylinder 90 can be compressed, thereby inputting air pressure to the box 16. The input air pressure can be positive pressure or negative pressure. When the piston 91 moves downward, positive pressure is input, and when it moves upward, negative pressure is input. The transformer interface can be set to be almost as large as the cross-sectional area of ​​the cylinder 90, so that the space of the cylinder 90 and the box 16 is almost the same space, and the internal air pressure can change synchronously with the movement of the piston 91.

[0059] Each embodiment of the incubator may also optionally include a gas replenishment system, which may be used in any of the aforementioned embodiments, and includes a gas supply system and a pressure relief device. The pressure relief device is used to release the gas in the box. The gas supply system is used to replenish the box with gas with stable process parameters while releasing the gas in the box, so as to ensure the stability of the process parameters of the gas in the box, reduce the fluctuation of the gas atmosphere in the box, and ensure a more stable culture environment. The process parameters include but are not limited to gas concentration, gas temperature, and gas humidity.

[0060] exist Figure 7In the embodiment shown, the gas supply system includes a gas source 19, a gas premixing tank 21, a gas source control device 20, and an air intake control device 24. The gas premixing tank 21 is used to mix various gases and adjust the process parameters of the gas. The gas source 19 provides the gas required for the culture environment, such as a mixed gas of oxygen, carbon dioxide and nitrogen. The gas source 19 provides one or more gases, such as nitrogen, carbon dioxide, oxygen, air or other gases. The gas premixing tank 21 has an input pipeline 210 and an output pipeline 211. The gas source control device 20 is arranged in the input pipeline 210, and the air intake control device is arranged in the output pipeline 211. The gas source control device 20 includes various valves, such as one or more of a pressure regulating valve and a control valve, and also includes one or more of a heating device, a humidifying device and a filtering device. The control valve is used to control the on-off of the pipeline, and the pressure regulating valve is used to control the output pressure of the pipeline. The control valve is preferably a one-way valve. The heating device is used to preheat the gas passing through the pipeline. The heating device can select a jacket heater, a coil heater or an electric heater. The humidifying device is used to add water vapor to increase the humidity of the gas in the pipeline. The method of adding water vapor can be to spray or inject water vapor into the pipe. The air intake control device 24 includes but is not limited to one or more of a pressure regulating valve and a control valve. The control valve is preferably a one-way valve. It can also optionally include a heating device, a humidifying device or a gas filter. The gas filter is preferably a high-efficiency gas filter with a pore size not greater than 0.3μm. A gas mixing device 22 and a gas control device 23 are arranged in the premixing tank 21. The mixing device 22 is activated when the gas source 19 provides a variety of gases. A fan or air pump can be selected to generate turbulence in the premixing tank 21. The gas control device 23 includes but is not limited to various types of gas concentration detectors, heating devices, humidifying devices, temperature and humidity detection devices, etc.

[0061] The gas comes out from the gas source 19, and after being adjusted by the gas source control device 20, it enters the premixing tank 21. The gas passes through the gas mixing device 22 to make the gas in the premixing tank 21 evenly mixed. After being adjusted to the set value by the gas control device 23 in the premixing tank 21, it can be ventilated to the incubator 16 through the air intake control device 24. According to the gas flow direction in the box 16, one or more air outlets 161 are set at the parts where the air flow is difficult to reach, and the active air release is achieved through the pressure relief device 27, and the air intake control device 24 is opened to replenish air. In order to further improve the uniformity and cleanliness of the gas inside the box 16, one or more gas mixing devices 25 are placed in the incubator 16 to make the gas in the box 16 in a turbulent state and thus maintain the uniformity of the gas. In order to accurately adjust the gas process parameters in the box 16, a gas control device 26 is also provided in the box 16. The gas control device 26 includes but is not limited to various gas concentration detectors, heating devices, humidification devices, temperature and humidity detection devices, etc.

[0062] Although the present invention is disclosed as above with preferred embodiments, it is not intended to limit the present invention. Any person skilled in the art may make possible changes and modifications without departing from the spirit and scope of the present invention. Therefore, any modification, equivalent change and modification made to the above embodiments according to the technical essence of the present invention without departing from the content of the technical solution of the present invention shall fall within the protection scope defined by the claims of the present invention.

Claims

1. An incubator, comprising a housing, characterized in that: It also includes a transformer, the box has a transformer interface, the transformer is connected to the box via the transformer interface, and the transformer is used to input fluctuating or constant air pressure to the box.

2. The incubator according to claim 1, characterized in that The pressure-changing device comprises a cylinder and a piston. The cylinder is connected to the box through the pressure-changing interface. The piston is movably arranged in the cylinder so as to input the gas pressure through the movement of the piston.

3. The incubator according to claim 2, characterized in that The cylinder body is connected to the pressure-changing interface directly or through a pipeline.

4. The incubator according to claim 2, characterized in that The cylinder body is directly connected to the pressure-changing interface, and the pressure-changing interface is large enough to enable the cylinder body and the box body to change the air pressure synchronously with the movement of the piston.

5. The incubator according to claim 2, characterized in that: The cylinder body is fixedly connected to the box body; preferably, the cylinder body is fixedly connected to the top of the box body.

6. The incubator according to claim 2, characterized in that The voltage conversion device also includes one of an electric cylinder, a gas cylinder, and a hydraulic cylinder; Preferably, the electric cylinder comprises a servo motor and a transmission mechanism, wherein the transmission mechanism connects the servo motor and the piston to convert the rotation of the servo motor into the movement of the piston; Preferably, the electric cylinder is an electric push rod, and the electric push rod can drive the piston to move.

7. The incubator according to claim 1, characterized in that The pressure-changing device comprises a gas storage tank, which is connected to the box body through a pressurizing gas path and a decompressing gas path; The boosting gas circuit is provided with a boosting pump and a boosting control valve, the boosting pump is used to transport the gas in the gas storage tank to the box body, and the boosting control valve is used to control the on-off of the boosting gas circuit; The decompression gas circuit is provided with a decompression pump and a decompression control valve, the decompression pump is used to transport the gas in the box to the gas storage tank, and the decompression control valve is used to control the on-off of the decompression gas circuit.

8. The incubator according to claim 1, wherein: The box body is also provided with a safety valve, which opens when the pressure in the box body exceeds a safety threshold.

9. The incubator according to claim 1, wherein: The box body also includes an openable and closable air inlet and an openable and closable air outlet which are independent of the voltage transformation interface.

10. The incubator according to claim 1, wherein: The box body is provided with a sterilizing device and / or a humidifying device and / or an electric heating device.

11. The incubator according to claim 10, characterized in that The sterilization device is an ultraviolet light emitter or a steam sterilization device.

12. The incubator according to claim 1, wherein: The gas in the incubator is one or more gases; preferably a three-gas incubator; more preferably, the gas is carbon dioxide, nitrogen, and oxygen.

13. The incubator according to any one of claims 1 to 12, characterized in that The incubator also includes an air supply system, which includes: A pressure relief device, used to release the gas in the box; and The gas supply system is used to provide gas with stable process parameters to the box while the pressure relief device is releasing.

14. The incubator according to claim 13, wherein: The box is provided with a gas mixing device for making the gas in the box in a turbulent state so as to maintain the uniformity of the gas.

15. The incubator according to claim 13, wherein: The gas supply system includes a premixing tank, which receives one or more gases through an input pipeline. The premixing tank is connected to the box through an output pipeline. A gas mixing device is provided in the premixing tank. The input pipeline is provided with a gas source control device for the gas source, and the output pipeline is provided with an air intake control device for the box.

16. The incubator according to claim 15, characterized in that A gas control device is also provided in the premixing tank, and the gas control device includes a gas concentration detector, a heating device, a humidifying device and / or a temperature and humidity detection device.

17. The incubator according to claim 16, wherein: The air source control device and / or the air intake control device comprises a heating device, a humidifying device and / or a gas filtering device.

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