Centrifuge tube reuse detection method and device for ultra-high-speed centrifuge

Abstract

The invention provides a centrifuge tube reuse detection method and device for an ultra-high-speed centrifuge, and the method comprises the following steps: S1, pressurizing and filling a new centrifuge tube with a medium, and carrying out the online detection of the diameter deformation condition of the centrifuge tube; s2, taking the maximum pressure value which can be borne by the centrifugal tube when the centrifugal tube returns to the initial diameter as a yield limit pressure value; s3, replacing the to-be-reused centrifugal tube, pressurizing and filling the to-be-reused centrifugal tube with a medium, and controlling the reuse pressure value to be 75-95% of the yield limit pressure value; s4, detecting the diameter deformation of the centrifuge tube to be reused on line; and S5, selecting a to-be-multiplexed centrifugal tube capable of returning to the initial diameter as a multiplexed centrifugal tube. The centrifugal tube can be detected, whether the centrifugal tube can be safely reused or not is judged, and the experiment cost is greatly reduced. The device can be used for detecting whether the replaced centrifuge tube meets the application requirements of the ultra-high-speed centrifuge. And the centrifugal tube with substandard quality is prevented from entering the ultra-high-speed centrifugal machine to cause equipment damage and even sample leakage.

Description

Technical Field

[0001] The invention relates to the technical field of centrifuge tubes, in particular to a centrifuge tube multiplexing detection method and device for ultra-high-speed centrifuges. Background Art

[0002] In the virus separation operation, an ultra-high-speed centrifuge with a rotation speed of 100,000 rpm is required to separate the virus at a maximum rotation speed of 100,000 rpm. The centrifuge tube needs to withstand a large gravitational acceleration. The existing centrifuge tubes used for the above working conditions are expensive and can only be used once, resulting in high experimental costs and great waste. If the centrifuge tube is fatigued at a high rotation speed, it is easy to break during the centrifugal operation, resulting in equipment damage or leakage of the test object. Summary of the invention

[0003] The technical problem to be solved by the present invention is to provide a method for detecting the reuse of centrifuge tubes for ultra-high-speed centrifuges, which can confirm whether the centrifuge tubes can be safely reused to reduce experimental costs.

[0004] In order to solve the above technical problems, the technical solution of the present invention is: A centrifuge tube multiplexing detection method for ultra-high speed centrifuge comprises the following steps: S1. Pressurize and fill the new centrifuge tube with medium, and detect the diameter deformation of the centrifuge tube online; S2, the maximum pressure that the centrifuge tube can withstand when it returns to its initial diameter is the yield limit pressure; S3, replace the centrifuge tube to be reused, pressurize and fill the medium into the centrifuge tube to be reused, and control the reuse pressure value to be 75%~95% of the yield limit pressure value; S4, online detection of diameter deformation of the centrifuge tube to be reused; S5. Select a centrifuge tube that can be restored to its original diameter and is intended to be reused as a reused centrifuge tube.

[0005] In a preferred embodiment, step S2 comprises the following steps: S21, pressurizing and filling the new centrifuge tube with the medium until the new centrifuge tube is destroyed; S22, recording the pressure value when the new centrifuge tube is destroyed, and recording it as the destruction limit pressure value; S23, take the value from 75% to 95% of the failure limit pressure value, which is the test yield limit pressure value; S24. Using the yield limit pressure value as the basic test data, increase or decrease the pressure value until the maximum pressure value that the centrifuge tube can withstand to return to its initial diameter is found.

[0006] In a preferred embodiment, the medium is air or water.

[0007] Another technical problem to be solved by the present invention is to provide a device for a method for detecting the reuse of centrifuge tubes for ultra-high-speed centrifuges, which can be used to accurately detect whether the centrifuge tubes can be reused.

[0008] In order to solve the above technical problems, the technical solution of the present invention is: A device for the above-mentioned centrifuge tube reuse detection method for ultra-high-speed centrifuges, comprising a plunger, a conduit is provided inside the plunger, and the plunger is used to form a sealing structure with the bottle mouth of the centrifuge tube; The conduit is connected to the output port of the pressure medium source; A pressure sensor is provided on the catheter; A detection device is also provided, and the detection device is used to detect the diameter change of the centrifuge tube.

[0009] In the preferred solution, the plunger is fixed on the base plate, bolts are also fixed on the base plate, and a pressure cover is also provided. The pressure cover is provided with holes corresponding to the bolts, and the bolts pass through the holes to be connected with nuts. The centrifuge tube is used to be arranged between the base plate and the pressure cover, and the mouth of the centrifuge tube is sleeved on the plunger, and a sealing ring is provided on the outer wall of the plunger.

[0010] In a preferred solution, a limiting ring is fixedly provided on the periphery of the plunger. The limiting ring is located on the periphery of the centrifuge tube orifice and is used to limit the deformation of the centrifuge tube orifice.

[0011] In a preferred solution, the structure of the pressure medium source is: the outlet of the pump is connected to the inlet of the pressure regulating valve, the outlet of the pressure regulating valve is connected to the inlet of the control valve, and the outlet of the control valve is connected to the conduit; The circulation port of the pressure regulating valve is connected to the inlet of the pump through a circulation pipe; A buffer tank is also provided on the catheter, and a compressed air bag is provided in the buffer tank.

[0012] In a preferred solution, the detection device includes an image detection device, a photoelectric array detection device, a rangefinder, a stroke detection device and a circumference detection device; The image detection device is used to capture an image of the centrifuge tube and detect a change in the diameter of the centrifuge tube based on the image; The photoelectric array detection device detects the diameter change of the centrifuge tube using an array of opposing light beams; The rangefinder uses laser or sonar to detect the distance between the transmitter and the outer wall of the centrifuge tube, and calculates the change in the diameter of the centrifuge tube based on the change in distance; The stroke detection device uses a detection head to contact the outer wall of the centrifuge tube, measures the displacement of the detection head in a mechanical, electromagnetic or photoelectric manner, and calculates the diameter change of the centrifuge tube according to the displacement of the detection head; The circumference detection device uses a detection belt wound on the outer wall of the centrifuge tube. One end of the detection belt is fixed, and the other end is connected to a fixed displacement sensor to detect the circumference change of the centrifuge tube and calculate the diameter change of the centrifuge tube based on the circumference change.

[0013] In a preferred solution, one end of the detection belt is fixedly connected to the bolt through a fixed connector, and the displacement sensor is fixedly connected to the current or another bolt; Displacement sensors include capacitive, grating or wound disc displacement sensors.

[0014] In a preferred solution, there are multiple plungers on the bottom plate, and the multiple plungers are all connected to the conduit; There are also multiple corresponding detection devices, and each detection device corresponds to a centrifuge tube.

[0015] The invention provides a method and device for detecting the multiplexing of centrifuge tubes for ultra-high-speed centrifuges, which have the following beneficial effects: 1. The method and device of the present invention can detect centrifuge tubes and determine whether the centrifuge tubes can be safely reused, thereby greatly reducing experimental costs.

[0016] 2. The method of the present invention can be used to detect whether the replaced centrifuge tube meets the application requirements of the ultracentrifuge, thereby preventing the centrifuge tubes that do not meet the quality standards from entering the ultracentrifuge, causing equipment damage or even sample leakage.

[0017] 3. The method of the present invention uses pressure parameters instead of other parameters for evaluation, which simplifies the detection and evaluation process and facilitates implementation.

[0018] 4. The device of the present invention evaluates the pressure bearing capacity of the centrifuge tube by the rebound of the centrifuge tube diameter deformation, so as to comprehensively evaluate the strength of the centrifuge tube and avoid unqualified centrifuge tubes from being used in ultra-high-speed centrifuges in a simple way.

[0019] 5. The device of the present invention controls the experimental pressure in a pressure cycle manner and balances the pressure fluctuation with a buffer tank, and can provide output pressure simply and accurately.

[0020] 6. The device of the present invention measures the diameter change of the centrifuge tube by the circumference method, which has low requirements on the measurement basis and can accurately obtain the circumference change of the centrifuge tube, thereby obtaining the diameter change, thereby ensuring the detection accuracy. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] The present invention will be further described below in conjunction with the accompanying drawings and embodiments: Figure 1 The present invention is a flow chart of the detection method.

[0022] Figure 2 The detection device of the present invention.

[0023] Figure 3 This is the multiple centrifuge tube detection device of the present invention.

[0024] Figure 4 It is a structural schematic diagram of the capacitive detection device of the present invention.

[0025] Figure 5 It is a schematic structural diagram of the grating type detection device of the present invention.

[0026] Figure 6 It is a schematic structural diagram of the winding disk type detection device of the present invention.

[0027] Figure 7 It is a schematic structural diagram of the pressure medium source of the present invention.

[0028] In the figure: gland 1, bolt 2, centrifuge tube 3, plunger 4, sealing ring 5, conduit 6, pressure medium source 7, circulation pipe 71, pump 72, pressure regulating valve 73, buffer tank 74, compression air bag 75, pressure sensor 8, control valve group 9, displacement sensor 10, first electrode layer 101, second electrode layer 102, damper 103, insulating section 104, electrode medium section 105, fixed connector 106, metal detection belt 107, grating head 108, grating guide rail 109, absolute value sensor 110, winding disk 111, detection belt 11, exhaust valve 12, bottom plate 13, limit ring 14. DETAILED DESCRIPTION

[0029] Embodiment 1: like Figure 1 As shown in, a method for multiplexing detection of centrifuge tubes for ultra-high-speed centrifuges comprises the following steps: S1, pressurizing and filling the new centrifuge tube 3 with the medium, and online detecting the diameter deformation of the centrifuge tube 3; The centrifuge tube 3 in this example is made of PVC or PC. In the preferred embodiment, the medium filled is air or water. In the optional embodiment, water is used as the medium filled in the centrifuge tube 3 made of PVC, and compressed air is used as the medium in the centrifuge tube 3 made of PC. PVC is the abbreviation of polyvinyl chloride, which is a thermoplastic plastic divided into two categories: soft PVC and hard PVC. In this example, hard PVC is used. PC is the abbreviation of polycarbonate, which is an engineering plastic with high strength and toughness.

[0030] S2, the maximum pressure value that the centrifuge tube 3 can withstand when returning to its initial diameter is the yield limit pressure value; In a preferred embodiment, step S2 comprises the following steps: S21, pressurizing and filling the new centrifuge tube 3 with a medium until the new centrifuge tube 3 is damaged; the damage of the centrifuge tube 3 means that the centrifuge tube 3 is deformed and cannot be restored, or cracks appear on the wall of the centrifuge tube 3. If any of the above situations occurs, the centrifuge tube 3 is judged to be damaged.

[0031] S22, recording the pressure value when the new centrifuge tube 3 is destroyed, and recording it as the destruction limit pressure value; S23. The value taken is 75%~95% of the failure limit pressure value, which is the test yield limit pressure value.

[0032] Preferably, different values ​​are taken as the test yield limit pressure values ​​for different materials. For PVC material, 80% of the destruction limit pressure value is taken as the yield limit pressure value, and for PC material, 90% of the destruction limit pressure value is taken as the yield limit pressure value.

[0033] S24, using the yield limit pressure value as the basic test data, increasing or decreasing the pressure value until the maximum pressure value that the centrifuge tube 3 can withstand when returning to the initial diameter is found.

[0034] S3. Replace the centrifuge tube 3 to be reused, pressurize and fill the medium into the centrifuge tube 3 to be reused, and control the reuse pressure value to be 75%~95% of the yield limit pressure value; for PVC material, in actual experiments, the experiment is carried out at 80% of the yield limit pressure value, and for PC material, the experiment is carried out at 90% of the yield limit pressure value. During the experiment, the pressure is allowed to fluctuate within 5% of the taken pressure value.

[0035] Preferably, the centrifuge tube 3 made of PVC material uses water as the pressure medium, while the centrifuge tube 3 made of PC material uses air as the pressure medium. This is because the breaking limit pressure value of the PC material is closer to the yield limit pressure value, and air has a certain buffer.

[0036] S4, online detection of the diameter deformation of the centrifuge tube 3 to be reused; S5. Select the centrifuge tube 3 to be reused that can be restored to its original diameter as the reused centrifuge tube.

[0037] In the above manner, it is determined whether the centrifuge tube 3 can be reused. The method of the present invention can also be used to determine the quality of the centrifuge tube 3 to avoid equipment damage or sample leakage caused by unqualified centrifuge tubes 3.

[0038] Embodiment 2: like Figure 2 , 3 As shown in the figure, a device for the above-mentioned ultra-high speed centrifuge centrifuge tube multiplexing detection method comprises a plunger 4, a conduit 6 is provided in the plunger 4, and the plunger 4 is used to form a sealing structure with the bottle mouth of the centrifuge tube 3; The conduit 6 is connected to the output port of the pressure medium source 7; A pressure sensor 8 is provided on the conduit 6; A detection device is also provided, which is used to detect the diameter change of the centrifuge tube 3. With this structure, when in use, the pressure medium is filled into the centrifuge tube 3 by the pressure medium source 7, and then the diameter change of the centrifuge tube 3 under different pressures is detected. With this structure, the centrifuge tube 3 can be easily tested for pressure resistance.

[0039] The preferred solution is Figure 2 , 3 As shown in the figure, the plunger 4 is fixed on the bottom plate 13, the bolt 2 is also fixed on the bottom plate 13, and a gland 1 is also provided. The gland 1 is provided with a hole corresponding to the bolt 2, and the bolt 2 passes through the hole and is connected with a nut. The centrifuge tube 3 is used to be arranged between the bottom plate 13 and the gland 1. The mouth of the centrifuge tube 3 is sleeved on the plunger 4, and a sealing ring 5 is provided on the outer wall of the plunger 4. With this structure, the centrifuge tube 3 is connected to the plunger 4 and is used to prevent the centrifuge tube 3 from being separated from the plunger 4.

[0040] The preferred solution is Figure 3 As shown in the figure, a limiting ring 14 is fixedly arranged on the periphery of the plunger 4, and the limiting ring 14 is located on the periphery of the mouth of the centrifuge tube 3 to limit the deformation of the mouth of the centrifuge tube 3. With this structure, the centrifuge tube 3 is prevented from being damaged at the position of the mouth of the centrifuge tube 3, and the mouth of the centrifuge tube 3 is also prevented from being deformed and causing leakage.

[0041] The preferred solution is Figure 7 As shown in , the structure of the pressure medium source 7 is: the outlet of the pump 72 is connected to the inlet of the pressure regulating valve 73, the outlet of the pressure regulating valve 73 is connected to the inlet of the control valve, and the outlet of the control valve is connected to the conduit 6; The circulation port of the pressure regulating valve 73 is connected to the inlet of the pump 72 through the circulation pipe 71; A buffer tank 74 is also provided on the catheter 6, and a compressed air bag 75 is provided in the buffer tank 74. The pump 72 in this example adopts a plunger pump to obtain a higher output pressure, for example, an output pressure of more than 2MPa. The pressure regulating valve 73 adopts a pilot pressure regulating valve, and the structure of the valve has a high pressure precision control. The control valve adopts an electrically controlled stop valve. In the preferred embodiment, the pressure regulating valve 73 adopts an electrically controlled pressure regulating valve, which can adjust the output pressure by automatic control, thereby improving the efficiency of the test and avoiding losses caused by operating errors.

[0042] The use of the pressure medium source 7 is to set the circulating pressure of the pressure regulating valve 73. When the output pressure of the pump 72 is higher than the set pressure, the medium enters the inlet of the pump 72 through the circulation pipe 71, and the buffer tank 74 is used to buffer the pressure fluctuation of the plunger pump. When in use, first set the circulating pressure of the pressure regulating valve 73, so that the pump 72 continuously supplies the pressure medium to the buffer tank 74 at a preset pressure until the pressure reaches the preset value, turn off the pump 72, and maintain the pressure by the one-way valve at the outlet of the pump 72. Detect whether the pressure of the pressure sensor 8 reaches the preset value. The pressure usually set will be higher than the pressure required for the experiment, because when the control valve is opened, a part of the pressure value will be lost. After opening the control valve, the experiment is completed, and the diameter of the centrifuge tube 3 is detected by the detection device. Then the exhaust valve 12 is depressurized, and a new centrifuge tube 3 is reinstalled for detection. Preferably, the control valve adopts an electric control valve to realize the automatic control test and improve the efficiency of the test.

[0043] In a preferred solution, the detection device includes an image detection device, a photoelectric array detection device, a rangefinder, a stroke detection device and a circumference detection device; In an optional scheme, the image detection device is used to capture a high-definition image of the centrifuge tube 3, and detect the diameter change of the centrifuge tube 3 based on the pixels of the image; during the detection, a high-definition camera, such as a 4k camera, is used to capture the initial image of the middle position of the centrifuge tube 3 and the image after filling with pressure medium. After calibration, the pixels of the image are corresponded to the diameter data, and the diameter change of the centrifuge tube 3 is obtained based on the pixel data by identifying the edge of the centrifuge tube 3.

[0044] In another optional scheme, the photoelectric array detection device uses a corresponding light beam array to detect the diameter change of the centrifuge tube 3; the centrifuge tube is detected by multiple array light beams, and when the diameter of the centrifuge tube 3 changes, more light beams are blocked, and the position of the light beam array is matched with the diameter data to obtain the diameter change of the centrifuge tube 3.

[0045] In another optional solution, the rangefinder uses laser or sonar to detect the distance between the transmitter and the outer wall of the centrifuge tube 3, and calculates the diameter change of the centrifuge tube 3 according to the distance change; the transmitter is fixed in position, and the transmitter first emits laser or sonar, and calculates the distance between the transmitter and the outer wall of the centrifuge tube 3 according to the flight time of the reflected light or reflected wave. The diameter change of the centrifuge tube 3 is calculated according to the change in the distance from the outer wall of the centrifuge tube 3 in the initial state of the test and after the pressure medium is filled.

[0046] In another optional scheme, the stroke detection device uses a detection head to contact the outer wall of the centrifuge tube 3, measures the displacement of the detection head in a mechanical, electromagnetic or photoelectric manner, and calculates the diameter change of the centrifuge tube 3 based on the displacement of the detection head; taking a micrometer as an example, the detection end of the micrometer is contacted with the outer wall of the centrifuge tube 3, and the position of the micrometer at this time is set to zero. When the diameter of the centrifuge tube 3 changes, the displacement of the micrometer detection rod is used to obtain the value of the diameter change.

[0047] For some plastics, such as PC, the breaking limit pressure value of PC is relatively close to the yield limit pressure value, so it is necessary to accurately detect the diameter change. The circumference detection device uses a detection belt 11 to be wound on the outer wall of the centrifuge tube 3. One end of the detection belt 11 is fixed, and the other end is connected to a fixed displacement sensor 10 to detect the circumference change of the centrifuge tube 3, and the diameter change of the centrifuge tube 3 is calculated based on the circumference change. With this structure, the detection accuracy is the highest, and the highest accuracy can reach nanometer level. Thereby, accurate detection results are obtained to avoid excessive numerical differences in pressure, damage to the centrifuge tube 3, or unqualified centrifuge tubes 3 passing the test.

[0048] The preferred solution is Figure 2 As shown in , one end of the detection belt 11 is fixedly connected to the bolt 2 through a fixed connector 106. Preferably, the detection belt 11 is a metal belt, the fixed connector 106 is a hook with a magnet, and the displacement sensor 10 is fixedly connected to the current or another bolt; that is, the displacement sensor 10 can be fixed on the current bolt or on another bolt, and the fixing method is preferably a clamp fixing method. Although the detection belt 11 is wound in a spiral shape on the outer wall of the centrifuge tube 3, the length change of the detection belt 11 is linearly related to the diameter change. Therefore, the diameter change of the centrifuge tube 3 can be accurately calculated based on the length change of the detection belt 11.

[0049] The displacement sensor 10 includes Figure 4 Capacitive, Figure 5 Raster or Figure 6 Wound disc displacement sensor.

[0050] like Figure 4As shown in , the structure of the capacitive displacement sensor 10 is that the detection belt 11 passes between the first electrode layer 101 and the second electrode layer 102, and an insulating section 104 and an electrode dielectric section 105 are provided on the detection belt 11, wherein the lengths of the insulating section 104 and the electrode dielectric section 105 are equal, and the lengths of the insulating section 104 and the electrode dielectric section 105 are slightly larger than the range of deformation of the circumference of the centrifuge tube 3. For example, the length of the insulating section 104 and the electrode dielectric section 105: the range of deformation of the circumference of the centrifuge tube 3 is 1.2:1. A damper 103 is also provided to provide damping for the detection belt 11. By detecting the capacitance change between the first electrode layer 101 and the second electrode layer 102, the deformation value of the circumference of the centrifuge tube 3 can be obtained, and then the diameter change of the centrifuge tube 3 can be calculated. The advantage of this structure is that it is very cheap and has sufficient detection accuracy.

[0051] like Figure 5 As shown in , the structure of the grating displacement sensor 10 is that the grating guide rail 109 is fixedly arranged, the grating head 108 is slidably connected to the grating guide rail 109, the grating head 108 is fixedly connected to the detection belt 11, and the circumference deformation of the centrifuge tube 3 drives the grating head 108 to move, thereby obtaining the deformation value of the circumference of the centrifuge tube 3, and then calculating the diameter change of the centrifuge tube 3. The structure of the grating displacement detection is the prior art. The advantage of this structure is that the detection accuracy is very high.

[0052] like Figure 6 As shown in , the structure of the winding disk displacement sensor is that the winding disk 111 is connected to the absolute value sensor 110 to drive the absolute value sensor 110 to rotate, thereby obtaining the rotation angle data of the winding disk 111, and the detection belt 11 is wound on the winding disk 111. The position of the winding disk 111 when it is wound on the centrifuge tube 3 is assumed to be the initial position, that is, the zero position. During the experiment, the diameter deformation of the centrifuge tube 3 drives the detection belt 11 to move, thereby driving the winding disk 111 to rotate. The deformation value of the circumference of the centrifuge tube 3 is calculated from the rotation angle data of the absolute value sensor 110, and then the diameter change of the centrifuge tube 3 is calculated. The advantage of this structure is that it is a commercially available product, easy to purchase, and suitable for small batch experiments.

[0053] Embodiment 3: Based on Example 2, the preferred solution is as follows Figure 3 In the embodiment, there are multiple plungers 4 on the bottom plate 13, and the multiple plungers 4 are all connected to the conduit 6; There are also multiple corresponding detection devices, each detection device corresponds to a centrifuge tube 3. With this structure, multiple centrifuge tubes 3 can be detected at one time.

[0054] When in use, the centrifuge tube 3 is installed on each plunger 4, and the pressure medium source 7 supplies the pressure medium. After reaching the preset value, the control valve group 9 is opened, that is, the conduit 6 fills the pressure medium into each centrifuge tube 3, and the detection device obtains the diameter change value of the centrifuge tube 3. Then, according to the method of Example 1, it is determined whether the centrifuge tube 3 can be reused. After the test is completed, the pressure medium is first discharged from the exhaust valve 12, and then the nut is loosened to remove the centrifuge tube 3.

[0055] The above-mentioned embodiments are only preferred technical solutions of the present invention and should not be regarded as limitations of the present invention. The embodiments and features in the embodiments of the present application can be arbitrarily combined with each other without conflict. The protection scope of the present invention shall be the technical solutions recorded in the claims, including the equivalent replacement solutions of the technical features in the technical solutions recorded in the claims. That is, equivalent replacement improvements within this scope are also within the protection scope of the present invention.

Claims

1. A method for multiplexing detection of centrifuge tubes for ultra-high speed centrifuges, characterized in that The following steps are involved: S1, pressurizing and filling a medium into a new centrifuge tube (3), and online detecting the diameter deformation of the centrifuge tube (3); S2, the maximum pressure value that the centrifuge tube (3) can withstand when returning to its initial diameter is the yield limit pressure value; S3, replacing the centrifuge tube (3) to be reused, and pressurizing the medium into the centrifuge tube (3) to be reused, wherein the reuse pressure value is controlled to be 75% to 95% of the yield limit pressure value; S4, online detection of diameter deformation of the centrifuge tube (3) to be reused; S5. Select the centrifuge tube (3) to be reused that can be restored to its original diameter as the reused centrifuge tube.

2. The method for multiplexing detection of centrifuge tubes for ultra-high-speed centrifuge according to claim 1, characterized in that: Step S2 includes the following steps: S21, pressurizing and filling the new centrifuge tube (3) with a medium until the new centrifuge tube (3) is destroyed; S22, recording the pressure value when the new centrifuge tube (3) is destroyed, and recording it as the destruction limit pressure value; S23, take the value from 75% to 95% of the failure limit pressure value, which is the test yield limit pressure value; S24. Using the yield limit pressure value as the basic test data, increase or decrease the pressure value until the maximum pressure value that the centrifuge tube (3) can withstand to restore its initial diameter is found.

3. The method for multiplexing detection of centrifuge tubes for ultracentrifuge according to claim 1, characterized in that: The medium is air or water.

4. A device for the ultracentrifuge tube multiplexing detection method according to any one of claims 1 to 3, characterized in that: It comprises a plunger (4), a conduit (6) is arranged inside the plunger (4), and the plunger (4) is used to form a sealing structure with the bottle mouth of the centrifuge tube (3); The conduit (6) is connected to the output port of the pressure medium source (7); A pressure sensor (8) is provided on the catheter (6); A detection device is also provided, and the detection device is used to detect the change in the diameter of the centrifuge tube (3).

5. The device for the method for multiplexing detection of centrifuge tubes for ultra-high-speed centrifuges according to claim 4, characterized in that: The plunger (4) is fixed on the bottom plate (13), a bolt (2) is also fixed on the bottom plate (13), and a gland (1) is also provided. The gland (1) is provided with a hole corresponding to the bolt (2), and the bolt (2) passes through the hole and is connected to a nut. The centrifuge tube (3) is used to be arranged between the bottom plate (13) and the gland (1), and the mouth of the centrifuge tube (3) is sleeved on the plunger (4). A sealing ring (5) is provided on the outer wall of the plunger (4).

6. The device for the method for multiplexing detection of centrifuge tubes for ultra-high-speed centrifuges according to claim 5, characterized in that: A limiting ring (14) is also fixedly provided on the periphery of the plunger (4). The limiting ring (14) is located on the periphery of the mouth of the centrifuge tube (3) and is used to limit deformation of the mouth of the centrifuge tube (3).

7. The device for the method for multiplexing detection of centrifuge tubes for ultra-high-speed centrifuges according to claim 5, characterized in that: The structure of the pressure medium source (7) is as follows: the outlet of the pump (72) is connected to the inlet of the pressure regulating valve (73), the outlet of the pressure regulating valve (73) is connected to the inlet of the control valve, and the outlet of the control valve is connected to the conduit (6); The circulation port of the pressure regulating valve (73) is connected to the inlet of the pump (72) through the circulation pipe (71); A buffer tank (74) is also provided on the catheter (6), and a compressed air bag (75) is provided in the buffer tank (74).

8. The device for the method for multiplexing detection of centrifuge tubes for ultra-high-speed centrifuges according to claim 5, characterized in that: The detection device includes an image detection device, a photoelectric array detection device, a rangefinder, a stroke detection device and a circumference detection device; The image detection device is used to capture an image of the centrifuge tube (3) and detect a change in the diameter of the centrifuge tube (3) based on the image; The photoelectric array detection device detects the diameter change of the centrifuge tube (3) using an array of opposing light beams; The rangefinder uses laser or sonar to detect the distance between the transmitter head and the outer wall of the centrifuge tube (3), and calculates the change in diameter of the centrifuge tube (3) based on the change in distance; The stroke detection device uses a detection head to contact the outer wall of the centrifuge tube (3), measures the displacement of the detection head in a mechanical, electromagnetic or photoelectric manner, and calculates the diameter change of the centrifuge tube (3) based on the displacement of the detection head; The circumference detection device uses a detection belt (11) wound around the outer wall of the centrifuge tube (3), one end of the detection belt (11) is fixed, and the other end is connected to a fixed displacement sensor (10) to detect the change in the circumference of the centrifuge tube (3), and calculate the change in the diameter of the centrifuge tube (3) based on the change in the circumference.

9. The device for the method for multiplexing detection of centrifuge tubes for ultra-high-speed centrifuges according to claim 8, characterized in that: One end of the detection belt (11) is fixedly connected to the bolt (2) via a fixed connector (106), and the displacement sensor (10) is fixedly connected to the current bolt or another bolt; The displacement sensor (10) comprises a capacitive, grating or winding disk displacement sensor.

10. The device for the method for multiplexing detection of centrifuge tubes for ultra-high-speed centrifuges according to any one of claims 5 to 9, characterized in that: There are a plurality of plungers (4) on the bottom plate (13), and each of the plurality of plungers (4) is connected to the conduit (6); There are also multiple corresponding detection devices, and each detection device corresponds to a centrifuge tube (3).

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