Method for detecting activity index of tea seeds

Abstract

The invention relates to the technical field of tea seed variety breeding, in particular to a method for detecting the activity index of tea seeds. According to the method, to-be-detected tea seeds are soaked in deionized water, the first conductivity R1 of the deionized water soaked with the tea seeds is obtained, the second conductivity R2 of the deionized water soaked with the tea seeds is obtained after a certain time, R3 is the conductivity of a tea seed-containing solution which is cooled to the room temperature after boiling, the deionized water soaked with the tea seeds is boiled, and the first conductivity R1 and the second conductivity R2 of the deionized water soaked with the tea seeds are obtained. And then cooling to room temperature, measuring the third conductivity R3 of the cooled deionized water, calculating a conductivity trend value # imgabs0 #, and judging the activity of the tea seeds according to the conductivity trend value.

Description

Technical Field

[0001] The invention relates to the technical field of tea seed variety breeding, and in particular to a method for detecting tea seed vitality indicators. Background Art

[0002] Tea seeds are the seeds of tea trees and are an important basis for tea production. The vitality of tea seeds refers to the ability of tea seeds to germinate and grow into seedlings under suitable conditions. It is an important indicator for measuring the quality and production potential of tea seeds. The level of tea seed vitality directly affects the quantity and quality of tea tree seedlings, and then affects the yield and quality of tea. At present, the commonly used methods for detecting tea seed vitality include the TTC standard germination test method, the accelerated aging test method, and the conductivity measurement method. Although these methods can reflect the level of tea seed vitality, they also have some disadvantages. For example, the standard germination test method requires a long time and more manual operations, and the accelerated aging test method requires special equipment and conditions.

[0003] The conductivity determination rule is to determine the vitality of the seeds by testing the conductivity value of the deionized water used to soak the seeds. The smaller the conductivity value, the greater the vitality. However, the conductivity value is greatly affected by factors such as water quality, temperature, and soaking time. Tea seeds that have lost their vitality can also have very low conductivity values. Therefore, the vitality of tea seeds cannot be determined based on a single conductivity value range. Summary of the invention

[0004] The purpose of the present invention is to avoid the shortcomings of the prior art and provide a method for detecting tea seed vitality indexes. The method for detecting tea seed vitality indexes can accurately judge the activity of tea seeds and has the advantage of being easy to operate.

[0005] To achieve the above object, the present invention provides the following technical solutions:

[0006] A method for detecting tea seed vitality index is provided, comprising the following steps:

[0007] The tea seeds to be tested are soaked in the deionized water, and the first conductivity R1 of the deionized water soaked with the tea seeds is first obtained. After a certain period of time, the second conductivity R2 of the deionized water soaked with the tea seeds is obtained.

[0008] R3 is the conductivity of the tea seed solution cooled to room temperature after boiling;

[0009] The boiling treatment is to destroy the cell membrane of the seeds and release the electrolytes in the cells into the water, thereby measuring the conductivity of the seeds under extreme conditions.

[0010] The deionized water soaked with tea seeds is boiled and then cooled to room temperature. The third conductivity R3 of the cooled deionized water is measured and the conductivity trend value is calculated.

[0011] The calculation formula is:

[0012]

[0013] When <0.5, the tea seed activity is judged as high activity;

[0014] When 0.5≤<0.7, the tea seed activity is judged as medium activity;

[0015] When ≥0.7, the tea seed activity is judged to be low.

[0016] In some embodiments, before the deionized water is soaked in the tea seeds to be tested, the following treatment is performed: the deionized water is covered with a film and kept at a constant temperature of 22° C. without light for 24 hours to provide a stable external environment.

[0017] In some embodiments, the instrument for measuring conductivity is a DDS-11A pointer conductivity meter.

[0018] In some embodiments, a detection step is further provided, wherein the detection test comprises: measuring a plurality of conductivities in a time period between a first conductivity and a second conductivity, wherein the detection time intervals of adjacent conductivities are the same, and when the difference between adjacent conductivities is within an allowable range, it indicates that the error of the obtained conductivity trend value is small, otherwise it indicates that the error of the obtained conductivity trend value is large.

[0019] In some embodiments, the allowable range of the difference is 4 to 5 μS cm -1 g -1 .

[0020] In some embodiments, the second conductivity is measured 7 to 12 hours after the first conductivity is measured.

[0021] In some embodiments, the number of tea seeds to be tested immersed in deionized water is ≥100.

[0022] Beneficial effects of the method for detecting tea seed vitality index of the present invention:

[0023] The method for detecting tea seed vitality index of the present invention obtains a first conductivity as an initial conductivity, a second conductivity as an end point conductivity, and a third conductivity under extreme conditions after boiling and cooling, obtains a conductivity trend value according to a calculation formula, and judges the tea seed vitality by the trend value, effectively overcoming the problem that the conductivity of deionized water for soaking tea seeds is affected by the external environment in the traditional method of measuring only the conductivity, avoiding the problem that tea seeds that have lost vitality also have low conductivity, ensuring the accuracy of the conductivity determination method, and having the advantage of easy operation. BRIEF DESCRIPTION OF THE DRAWINGS

[0024] Figure 1 The conductivity changes of KJ in three different storage methods

[0025] Figure 2 The conductivity changes of FH in three storage methods

[0026] Figure 3 The conductivity changes of three types of YS storage DETAILED DESCRIPTION

[0027] The preferred embodiments of the present invention will be described in more detail below with reference to the accompanying drawings. Although the preferred embodiments of the present invention are shown in the accompanying drawings, it should be understood that the present invention can be implemented in various forms and should not be limited by the embodiments described herein. On the contrary, these embodiments are provided to make the present invention more thorough and complete, and to fully convey the scope of the present invention to those skilled in the art.

[0029] Example

[0030] The method for rapidly detecting tea seed vitality index disclosed in this embodiment is only applicable to sampling a batch of tea seeds. In actual application, the remaining tea seeds after sampling can be used normally for production needs.

[0031] The method comprises the following steps: soaking tea seed samples in deionized water in groups, measuring the initial conductivity of the tea seeds (first conductivity R1), keeping the tea seeds in the deionized water at a constant temperature (using a constant temperature box environment, specifically: a temperature of 22° C. in a lightless environment), allowing the tea seeds to absorb water and swell, and using a conductivity meter to measure the conductivity of deionized water in a beaker at different time periods (such as 1 h, 2 h, 4 h, 6 h, 8 h, and 10 h), wherein 10 h is the endpoint conductivity (second conductivity R2) and recording the data, and then transferring the tea seeds to a boiling water bath for 1 h, and measuring the conductivity (third conductivity R3) after cooling to room temperature.

[0032]

[0033] According to the trend of relative conductivity, each sample was divided into three levels: high activity (trend less than 0.5), medium activity (trend greater than or equal to 0.5 and less than 0.7), and low activity (trend greater than or equal to 0.7).

[0034] The vitality level of tea seeds is divided according to this trend. The tea seeds are taken out, washed and soaked with clean water, germinated, and then sown. After sowing, the germination rate is observed and recorded to detect the vitality of the tea seeds. The germination method is warm water soaking.

[0035] Among them, the number of tea seed samples is 100

[0036] The conductivity obtained after 1h, 2h, 4h, 6h, 8h, and 10h is calculated, and the difference between adjacent conductivities is calculated. The difference is allowed to be within 5μS cm-1 g -1 The accuracy of the conductivity trend value can be monitored in real time through the difference calculation.

[0037] The system for detecting tea seed vitality includes the following components:

[0038] (1) Deionized water or distilled water;

[0039] (2) DDS-11A pointer conductivity meter;

[0040] (3) WPS or IBM SPSS Statistical 23 processing equipment;

[0041] (4) Storage devices used to process and store data.

[0042] WPS and IBM SPSS Statistics 23 were used to analyze the conductivity data and determine the viability of the seeds. The storage device was used to store the data and software programs collected from the DDS-11A pointer conductivity meter.

[0043] The present application relates to the technical field of seed detection, and provides a method for detecting tea seed vitality detection indicators, comprising the following steps: soaking tea seeds in deionized water; measuring the conductivity of seeds and water; and evaluating the vitality of tea seeds according to the conductivity trend value. The present invention can quickly distinguish the vitality of tea seeds by detecting the conductivity change trend, so as to quickly detect seed quality. The present invention has the advantages of simple, rapid, non-destructive operation, reliable and intuitive results, and is suitable for large-scale tea seed detection, and has wide application value.

[0044] Test example

[0045] Experimental Materials

[0046] On November 21, 2023, seeds of 'Phoenix Dancong', 'Micui Lu', and 'Phoenix Narcissus' (FH, KJ, YS) with cracked and mature peels were picked in the tea garden of South China Agricultural University and placed in a ventilated place in the laboratory for 2-3 days until the peels fell off for experiments.

[0047] Experimental methods

[0048] Sampling: 1100 tea seeds of the same variety (a total of 3300 tea seeds, 3.3 kg) were taken and stored in three different ways (wet sand storage, low temperature storage, and indoor cool storage). In each stage, 180 seeds were taken and divided into 4 groups for vitality testing using relative conductivity testing.

[0049] Relative conductivity detection:

[0050] Fifteen seeds were randomly selected for each treatment, with 5 seeds in each group, for a total of three groups. After being rinsed with deionized water (or distilled water) three times, they were immersed in a beaker filled with 200 ml of deionized water to measure the conductivity R1. The beaker filled with only deionized water was used as a control and the conductivity R was measured. The conductivity R2 was measured after being sealed with tin foil and left to stand for 10 hours at room temperature. Then, they were transferred to a boiling water bath for 1 hour, cooled to room temperature, and the conductivity was measured separately, recorded as R3. The value of R remained unchanged during this process, proving that the experimental data is available.

[0051] Relative conductivity (%) = (R2-R1) / (R3-R1) x 100% [7]

[0052] Germination rate determination

[0053] Randomly select 20 tea seeds from each treatment, soak them for 1 day, and maintain the water temperature at 25-30℃. After soaking, heat and germinate them, and cover them with soil at a depth of 3-5cm.

[0054] Tea seed germination rate (%) = number of germinated seeds / number of test seeds × 100%

[0055] Tea seed storage method

[0056] Wet sand storage: Add water to sterile sand to 60%-70% of its maximum water holding capacity (hold the sand tightly with your hands, and let it spread naturally after loosening, but without dripping water). Mix the tea seeds that have been sterilized with alcohol cotton balls with the wet sand evenly into an acrylic bucket and place it in a ventilated, cool and dry place. During storage, regularly check the humidity of the sand and the state of the seeds.

[0057] Low temperature storage: After disinfecting the tea seeds with alcohol cotton balls, put them in an aluminum foil bag and store them in the refrigerator (4°C).

[0058] Cool indoors: Spread the tea seeds flat on a laboratory table in a ventilated, dry and cool place, and turn them over every 10 days.

[0059] Changes in germination rate of different tea varieties during storage

[0060] As shown in Table 1, under wet sand storage conditions, the germination rate of tea seeds of three different varieties showed similar trends with storage time, basically showing a slow increase, then decrease, and finally an increase trend with the increase of storage time. The germination rate of FH reached 45% at 40 days of storage, and its germination rate still showed an upward trend after 80 days of storage, indicating that this variety has higher vitality and a later germination peak in wet sand storage. The germination rate of KJ dropped to 40% after 80 days of storage, indicating that its vitality was relatively low and failed to recover to the initial level within the experimental period. The germination rate of YS reached a maximum value of 50% after 80 days of storage, indicating that under the same storage conditions, this variety has the best germination performance.

[0061] Under low temperature storage conditions, the germination rates of three different varieties of tea seeds showed significant differences with storage time. The three tea seeds showed a higher germination rate at the beginning of storage and a tendency to recover in the middle, indicating that tea seeds have a good adaptability to low temperature storage conditions.

[0062] Under indoor cooling conditions, all three seeds lost their germination characteristics after being stored for 25-40 days.

[0063] Table 1 Changes in germination rate of three varieties stored in wet sand

[0064]

[0065]

[0066] Changes of conductivity index of different tea varieties during storage

[0067] The relative conductivity of seed extract is an important indicator for evaluating the integrity of seed cell membranes, reflecting the degree of damage and functional status of cell membranes. The higher the relative conductivity, the higher the degree of damage and the lower the seed vitality. Figure 1-3 As shown in the figure, the relative conductivity increases with the storage time, and the relative conductivity of the seed extract in air-dried storage (indoor cooling) is higher than that in the other two storage methods. The relative conductivity under low-temperature storage conditions in FH and YS states is significantly higher than that in wet sand storage, while the relative conductivity under wet sand storage conditions in KJ state is basically higher than that under low-temperature storage conditions. The possible reason is that the tea seeds and wet sand in KJ state were not fully disinfected, resulting in mildew of the tea seeds and increased damage to the cell membrane.

[0068] Correlation analysis of activity indicators of various varieties

[0069] Correlation analysis involves studying two or more interrelated data elements in order to assess the degree of interdependence between these data elements. When two variables show a significant correlation, the change in one variable can be used to infer the change dynamics of the other variable. As shown in Tables 2-4, correlation analysis was performed on the two vitality indicators of moisture content and germination rate of the three varieties and the three physical and chemical indicators of moisture content, dehydrogenase activity, and relative conductivity. The results showed that the germination rate of KJ and FH was extremely significantly positively correlated with moisture content and dehydrogenase activity; the moisture content was significantly positively correlated with dehydrogenase activity, and negatively correlated with relative conductivity but not significantly. However, the germination rate of YS was significantly negatively correlated with relative conductivity, and the germination rate was extremely significantly positively correlated with moisture content and dehydrogenase activity; the moisture content was extremely significantly correlated with dehydrogenase activity, and negatively correlated with relative conductivity. From the analysis, it can be seen that the changes in the physical and chemical indicators of tea seeds during storage are closely related to the changes in tea seed vitality. Therefore, the dehydrogenase activity, moisture content and relative conductivity during storage can be used as indicators to measure the vitality level of tea seeds.

[0070] Table 2 Correlation between KJ germination rate / water content and other activity indicators

[0071]

[0072]

[0073] Note: “*” indicates significant correlation and “**” indicates extremely significant correlation

[0074] Table 3 Correlation between FH germination rate / water content and other activity indicators

[0075]

[0076] Note: “*” indicates significant correlation and “**” indicates extremely significant correlation

[0077] Table 4 Correlation between YS germination rate / water content and other activity indicators

[0078]

[0079] Note: “*” indicates significant correlation and “**” indicates extremely significant correlation

[0080] The vitality of seeds of three tea varieties under different storage methods was tested by relative conductivity detection method. Through statistical analysis of experimental data, it can be concluded that the change of relative conductivity of tea seed extract reflects the integrity and functional state of seed cell membrane. In the late stage of wet sand storage and low temperature storage, the relative conductivity is low, which can indicate that the integrity of tea seed cell membrane is good, and the corresponding germination rate can be improved after necessary germination treatment; while in air-dried storage, the relative conductivity is high, indicating that the seed cell membrane is damaged and the activity is reduced, which shows that the storage environment has a significant effect on the integrity of seed cell membrane, among which the YS relative conductivity is significantly negatively correlated with the germination rate, and the higher conductivity may indicate that the structure of seed cell membrane is damaged, thus affecting the vitality and germination ability of seeds.

[0081] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. For those skilled in the art, the present invention may have various modifications and variations. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention shall be included in the protection scope of the present invention.

Claims

1. A method for detecting tea seed vitality index, characterized in that: The following steps are involved: Soak the tea seeds to be tested in deionized water, first obtain the first conductivity R1 of the deionized water soaked with the tea seeds, and then obtain the second conductivity R2 of the deionized water soaked with the tea seeds after a certain period of time. The deionized water soaked with tea seeds was boiled and then cooled to room temperature. The third conductivity R3 of the cooled deionized water was measured and the conductivity trend value was calculated. The calculation formula is: when The tea seed vitality is judged to be high activity; when The tea seed activity is judged to be moderate; when The tea seed activity is judged to be low.

2. The method for detecting tea seed vitality index according to claim 1, characterized in that: Before the deionized water is used to soak the tea seeds to be tested, the following treatment is performed: the deionized water is covered with a film and kept at a constant temperature of 22° C. without light for 24 hours.

3. The method for detecting tea seed vitality index according to claim 1, characterized in that: The instrument for measuring conductivity is a DDS-11A pointer-type conductivity meter.

4. The method for detecting tea seed vitality index according to claim 1, characterized in that: A detection step is also provided, and the detection test comprises: Several conductivities are measured in the time period between the first conductivity and the second conductivity, and the detection time intervals of adjacent conductivities are the same. When the difference between adjacent conductivities is within the allowable range, it indicates that the error of the obtained conductivity trend value is small, otherwise it indicates that the error of the obtained conductivity trend value is large.

5. The method for detecting tea seed vitality index according to claim 4, characterized in that: The allowable range of the difference is 4 to 5 μS cm -1 g -1 .

6. The method for detecting tea seed vitality index according to claim 1, characterized in that: The second conductivity is measured 7 to 12 hours after the first conductivity is measured.

7. The method for detecting tea seed vitality index according to claim 1, characterized in that: The number of tea seeds to be tested immersed in deionized water is ≥100.

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