Full-automatic bacterial endotoxin dilution detection device and system

Abstract

The invention relates to the technical field of biomedical detection, and particularly discloses a full-automatic bacterial endotoxin dilution detection system and a detection method thereof. The system aims at the operation complexity and the cross contamination risk in the bacterial endotoxin (lipopolysaccharide LPS) detection process, and full automation of endotoxin detection is achieved. The system comprises a dilution module, a sample adding module, a reagent distribution module, an incubation module, a detection module and a central control unit. Through a preset program, the system can automatically complete multi-stage dilution of a sample, accurate sample adding, LAL reagent adding, constant-temperature incubation and real-time monitoring of a reaction process and result interpretation. The system improves the detection efficiency and accuracy, reduces manual operation errors and cross contamination risks, supports simultaneous treatment of multiple samples, and flexibly adapts to different detection requirements. By implementing the method, an efficient and accurate solution is provided for endotoxin detection in the fields of medicine production, food safety, medical instruments and the like.

Description

Technical Field

[0001] The present invention relates to the field of life sciences, and in particular to a fully automatic bacterial endotoxin dilution detection device and system. Background Art

[0002] Bacterial endotoxin, as a lipopolysaccharide (LPS) component released from the cell wall of Gram-negative bacteria, has extremely high biological activity. Even at extremely low concentrations, it may trigger a strong immune response in the host, so its detection is of great significance in many fields such as biomedicine, drug production, food safety and medical devices.

[0003] Currently, the horseshoe crab test (LAL method) is widely used as the standard method for endotoxin detection. However, this method is complicated to implement and requires multiple precise dilutions and time control, which greatly depends on the operator's skills and experience. This not only leads to instability in test results and increases the risk of human error, but also increases the possibility of cross contamination due to frequent manual operations.

[0004] In view of the above technical defects, there is an urgent need to develop a more efficient, accurate and human-interference-reducing endotoxin detection system. A fully automated detection system can not only significantly improve detection efficiency, but also reduce the risk of cross-contamination by reducing the chance of manual contact with samples, thereby enhancing the accuracy and reliability of the test results. Therefore, the research and development of a fully automated bacterial endotoxin dilution detection system came into being to address the various deficiencies in existing technologies.

[0005] In order to solve the above problems, the applicant proposes a fully automatic bacterial endotoxin dilution detection device and system. Summary of the invention

[0006] The object of the present invention is to provide a fully automatic bacterial endotoxin dilution detection device and system to solve the problems in the prior art.

[0007] To achieve the above objectives, the present invention provides the following technical solutions: 1. A fully automatic bacterial endotoxin dilution detection device and system, comprising:

[0008] Dilution module, which can automatically complete multi-level dilution of samples according to preset programs;

[0009] A sample adding module is used to accurately add the diluted sample into the reaction plate;

[0010] A reagent dispensing module, used for adding LAL reagent to the reaction plate;

[0011] An incubation module, capable of providing a constant temperature environment for the reaction in the reaction plate;

[0012] A detection module, used to monitor the reaction process in real time and interpret the results;

[0013] And a central control unit is used to coordinate the work of the dilution module, the sample addition module, the reagent distribution module, the incubation module and the detection module, and to perform data processing and result output.

[0014] Optionally, the dilution module includes one or more dilution units, each of which can automatically dilute the sample according to a preset dilution ratio.

[0015] Optionally, the sample adding module includes a precise pipetting device to ensure that the diluted sample is accurately added to a designated position in the reaction plate in a predetermined volume.

[0016] Optionally, the reagent dispensing module includes one or more reagent channels, each of which can automatically add LAL reagent to the reaction plate according to a preset reagent volume.

[0017] Optionally, the incubation module includes a heating device and a temperature control system, which can provide a constant and suitable reaction temperature.

[0018] Optionally, the detection module includes an optical detection device or an electrochemical detection device for monitoring the reaction process in real time and interpreting the result based on the reaction signal.

[0019] Optionally, the central control unit includes a processor and a memory, the processor is used to execute the detection program, and the memory is used to store the detection program and data.

[0020] A fully automatic bacterial endotoxin dilution detection system comprises the fully automatic bacterial endotoxin dilution detection device as described in any one of claims 1 to 7, and a display unit and / or printing unit connected thereto for displaying and / or printing the detection results.

[0021] Optionally, an input unit is also included for inputting detection parameters and / or program instructions.

[0022] Optionally, the system can process multiple samples simultaneously and perform dilution, sample loading, reagent addition, incubation and detection separately.

[0023] Beneficial effects: 1. Significantly improve detection efficiency: Through automated means, the entire process of endotoxin detection can be completed quickly and accurately, greatly improving detection efficiency.

[0024] 2. Reduce human operation errors: Reduce the human intervention link, reduce the impact of human operation errors on the test results, and improve the accuracy and repeatability of the test results.

[0025] 3. Reduce the risk of cross contamination: By optimizing the sample handling and reagent distribution process, the chance of manual contact with the sample is reduced, thereby reducing the risk of cross contamination.

[0026] 4. Improve detection throughput: The system can process multiple samples simultaneously, meeting the needs of large-scale detection.

[0027] 5. Flexible adaptation to different testing needs: Through flexible program setting functions, the system can adapt to diverse testing needs and provide users with more convenient and efficient testing solutions. BRIEF DESCRIPTION OF THE DRAWINGS

[0028] Figure 1 It is a schematic diagram of the structure of an embodiment of the present invention;

[0029] Figure 2 It is a schematic diagram of the internal structure of an embodiment of the present invention. DETAILED DESCRIPTION

[0031] The following describes the preferred embodiments of the present invention with reference to the drawings in the specification, so that the technical content is clearer and easier to understand. The present invention can be embodied in many different forms of embodiments, and the protection scope of the present invention is not limited to the embodiments mentioned in the text.

[0032] In the drawings, components with the same structure are indicated by the same numerical reference numerals, and components with similar structures or functions are indicated by similar numerical reference numerals. The size and thickness of each component shown in the drawings are arbitrarily shown, and the present invention does not limit the size and thickness of each component. In order to make the illustration clearer, the thickness of the components is appropriately exaggerated in some places in the drawings.

[0033] Example 1

[0034] The core structure of the fully automatic bacterial endotoxin dilution detection device

[0035] Dilution module: The dilution module is the first step in the entire detection process, and its design reflects a high degree of automation and intelligence. By integrating sophisticated liquid handling technology and advanced control algorithms, the module can automatically complete multi-stage dilution of samples according to preset procedures. In specific operations, users only need to place the sample to be tested in the specified position and set the dilution ratio and steps. The dilution module can automatically complete the entire process from sampling, dilution to discharge of waste liquid. This process not only ensures the accuracy and consistency of sample dilution, but also greatly improves detection efficiency.

[0036] Sample loading module: The sample loading module is equipped with a high-precision pipetting device and a precision positioning system, which can accurately add the diluted sample to the reaction plate. In order to further improve the accuracy and stability of sample loading, the module also uses advanced vibration control and bubble elimination technology to effectively avoid the influence of bubbles generated during the sample loading process on the test results. In addition, the sample loading module also has a variety of sample loading modes to choose from to meet the detection needs of different samples.

[0037] Reagent dispensing module: The reagent dispensing module is a key link to ensure the smooth progress of the reaction. This module can not only automatically identify different types of LAL reagents, but also accurately distribute the reagent volume according to the detection requirements. To achieve this goal, the reagent dispensing module uses sophisticated liquid dispensing technology and an intelligent recognition system to accurately identify the type and concentration of reagents and accurately distribute the reagent volume to each reaction well according to the preset program. This process not only ensures the accuracy and consistency of the reagent volume, but also greatly reduces the detection error caused by improper reagent distribution.

[0038] Incubation module: The incubation module provides a constant temperature and stable incubation environment for the reaction. By integrating advanced temperature control technology and humidity regulation system, the module can accurately control the incubation temperature and humidity to ensure that the reaction is carried out under optimal conditions. In addition, the incubation module also has automatic calibration and fault alarm functions, which can monitor and adjust the incubation environment in real time to ensure the stability and reliability of the reaction.

[0039] Detection module: The detection module integrates high-precision sensors and data processing algorithms, which can monitor the reaction process in real time and automatically interpret the results. The module uses advanced optical detection technology or electrochemical detection technology to accurately measure the signal changes generated during the reaction process and judge the concentration of endotoxins based on this. In order to improve the accuracy and sensitivity of the detection, the detection module also uses multi-channel detection technology and automatic calibration function, which can detect multiple samples at the same time and automatically eliminate background interference.

[0040] Central Control Unit: As the "brain" of the entire system, the central control unit is responsible for coordinating the work of each module, and performing data processing and result output. The unit uses a high-performance microprocessor and embedded operating system, which can receive and process data information from each module in real time, and make automatic judgments and decisions according to preset programs. In addition, the central control unit also has flexible program setting functions and user interface design, which can provide users with a more convenient and efficient detection experience.

[0041] The above shows and describes the basic principles and main features of the present invention and the advantages of the present invention. It is obvious to those skilled in the art that the present invention is not limited to the details of the above exemplary embodiments, and the present invention can be implemented in other specific forms without departing from the spirit or basic features of the present invention. Therefore, no matter from which point of view, the embodiments should be regarded as exemplary and non-restrictive. The scope of the present invention is defined by the attached claims rather than the above description, and it is intended that all changes falling within the meaning and scope of the equivalent elements of the claims are included in the present invention. Any figure mark in the claims should not be regarded as limiting the claims involved.

[0042] In addition, it should be understood that although the present specification is described according to implementation modes, not every implementation mode contains only one independent technical solution. This description of the specification is only for the sake of clarity. Those skilled in the art should regard the specification as a whole. The technical solutions in each embodiment may also be appropriately combined to form other implementation modes that can be understood by those skilled in the art.

Claims

1. A fully automatic bacterial endotoxin dilution detection device and system, characterized in that: include: Dilution module, which can automatically complete multi-level dilution of samples according to preset programs; A sample adding module is used to accurately add the diluted sample into the reaction plate; A reagent dispensing module, used for adding LAL reagent to the reaction plate; An incubation module, capable of providing a constant temperature environment for the reaction in the reaction plate; A detection module, used to monitor the reaction process in real time and interpret the results; And a central control unit is used to coordinate the work of the dilution module, the sample addition module, the reagent distribution module, the incubation module and the detection module, and to perform data processing and result output.

2. The fully automatic bacterial endotoxin dilution detection device according to claim 1, characterized in that: The dilution module includes one or more dilution units, each of which can automatically dilute the sample according to a preset dilution ratio.

3. The fully automatic bacterial endotoxin dilution detection device according to claim 1, characterized in that: The sample adding module includes a precise pipetting device to ensure that the diluted sample is accurately added to a designated position in the reaction plate in a predetermined volume.

4. The fully automatic bacterial endotoxin dilution detection device according to claim 1, characterized in that: The reagent dispensing module includes one or more reagent channels, each of which can automatically add LAL reagent to the reaction plate according to a preset reagent volume.

5. The fully automatic bacterial endotoxin dilution detection device according to claim 1, characterized in that: The incubation module includes a heating device and a temperature control system, which can provide a constant and suitable reaction temperature.

6. The fully automatic bacterial endotoxin dilution detection device according to claim 1, characterized in that: The detection module includes an optical detection device or an electrochemical detection device, which is used to monitor the reaction process in real time and interpret the result according to the reaction signal.

7. The fully automatic bacterial endotoxin dilution detection device according to claim 1, characterized in that: The central control unit includes a processor and a memory, wherein the processor is used to execute the detection program, and the memory is used to store the detection program and data.

8. A fully automatic bacterial endotoxin dilution detection system, characterized in that: It comprises a fully automatic bacterial endotoxin dilution detection device as described in any one of claims 1 to 7, and a display unit and / or printing unit connected thereto, for displaying and / or printing the detection results.

9. The fully automatic bacterial endotoxin dilution detection system according to claim 8, characterized in that: The device also comprises an input unit for inputting detection parameters and / or program instructions.

10. The fully automatic bacterial endotoxin dilution detection system according to claim 8, characterized in that: The system can process multiple samples simultaneously and perform dilution, sample loading, reagent addition, incubation and detection separately.

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