Composition for preventing radioactive skin injury

Abstract

The invention discloses a composition for preventing radioactive skin injury. The composition comprises independently packaged vegetable oil dissolved with ozone and procyanidine, the content of ozone in the vegetable oil is 1-100mg / 100mL, and the vegetable oil dissolved with ozone is prepared by the following method: continuously introducing ozone into the vegetable oil, and stopping introducing ozone until the content of ozone in the vegetable oil reaches the standard to obtain the vegetable oil dissolved with ozone. The composition for preventing the radioactive skin injury can be applied to preparation of medicines for resisting the radioactive skin injury. After the ozone oil and the procyanidine are combined for use, an obvious relieving effect on skin injury is achieved, meanwhile, abnormal blood flow of a radiation part can be prevented, normal blood supply of the radiation part can be maintained, and repairing of the skin injury can be effectively promoted.

Description

Technical Field

[0001] The invention relates to a preventive medicine for skin damage, in particular to a composition for preventing radiation skin damage. Background Art

[0002] Radiation-Induced Skin Injury (RISI) is the most common complication of radiotherapy. Among patients with tumors who receive radiotherapy, 95% of them will have varying degrees of radiation-induced skin injury, especially patients with breast cancer, lung cancer, and head and neck tumors. About 49% of patients with head and neck radiotherapy will have grade 3 or 4 radiation-induced skin injury during treatment, and 20% to 25% of patients will have severe wet reactions and ulcers. According to the RTOG acute radiation injury grading standard, the degree of acute skin damage during radiotherapy is divided into 5 levels: grade 0 is no skin change; grade 1 is erythema, the skin in the radiation field is slightly red, and sometimes itchy; grade 2 is pigmentation, black and dry, with skin cracks and dry peeling; grade 3 is dry reaction, the skin is obviously pigmented, black and dry, with skin cracks and dry peeling; grade 4 is wet reaction, severe pigmentation, skin edema, exudation, and ulceration may occur.

[0003] In particular, the intense thermal pain and mechanical pain hypersensitivity after radiotherapy often cause patients to feel extremely uncomfortable. This pain may cause anxiety and depression in patients, seriously affecting their quality of life and treatment compliance. 58.1% of patients interrupted radiotherapy because they could not tolerate the pain associated with radiation-induced skin damage, which seriously affected the treatment effect of patients with malignant tumors.

[0004] The prevention and treatment of radiation-induced skin damage has always been a concern in the radiotherapy community. However, to date, there is still no unified standard or specific drug for radiation-induced skin damage, and the existing treatments are also ineffective. Therefore, it is urgent to explore the mechanism of radiation-induced skin damage and develop corresponding intervention measures, and there is an urgent need for efficient and safe solutions to prevent and treat radiation-induced skin damage.

[0005] Medical ozone is further prepared on the basis of medical pure oxygen. It has been used for more than 100 years. It has bactericidal, anti-inflammatory, antioxidant, analgesic and immunomodulatory effects. At appropriate concentrations, ozone is considered to be an ideal drug and has been widely used in the treatment of vascular diseases, degenerative diseases, chronic infections, acute and chronic soft tissue injuries and other diseases. Studies have found that ozone can enhance the phagocytic function of macrophages by activating AMPK, thereby alleviating CCI-induced neuropathic pain.

[0006] Medical ozone can remove bacteria and dirt from the body surface, reduce tissue fluid exudation, activate immune cells, regulate interferon, interleukin, tumor necrosis factor-α, fibroblast growth factor, epidermal growth factor and other cytokines, promote collagen fiber production, and accelerate wound healing. In the process of wound healing, ozone oil increases fibroblast proliferation and migration by enhancing miR-21-5p-mediated RASA1 inhibition, thereby promoting wound healing. Medical ozone can also increase the content of 2,3-diphosphoglyceric acid and adenosine triphosphate in red blood cells, improve their oxygen carrying capacity, shift the oxygen dissociation curve to the right, and make oxygenated hemoglobin more easily enter ischemic tissues, thereby increasing tissue oxygen supply. At the same time, the reactive oxygen produced by ozone decomposition stays in the surrounding tissues, directly improving the hypoxic state, promoting the opening and functional recovery of capillaries, and facilitating wound healing.

[0007] However, the strong oxidizing property of medical ozone will aggravate the peroxidation in radiation-induced skin damage and accelerate oxidative stress. Ozone can enter the subcutaneous tissue through sweat glands and sebaceous glands and react rapidly within 1-2 minutes. After radiation-induced skin damage, a large amount of peroxides will be produced due to the direct cytotoxicity and inflammatory response of the radiation. These peroxides will further aggravate the inflammatory response and cell damage, leading to further development of skin damage:

[0008] 1. Oxidation of ozone

[0009] Ozone is a strong oxidant that can react quickly with other substances to cause oxidative stress in the body. Ozone reacts with polyunsaturated fatty acids (PUFA) and water to produce reactive oxygen species (ROS) such as hydrogen peroxide (H2O2), and lipid ozone oxides (LOPs), including lipid oxygen free radicals, hydroperoxides, and ozonated olefin compounds.

[0010] 2. The dual role of ozone

[0011] The effect of ozone depends on its concentration, dosage, route and site of action. Rational use of ozone can induce mild oxidative stress response and improve the body's antioxidant capacity, while improper use may induce excessive oxidation reaction, consume antioxidant enzymes, produce toxic substances and even induce cell necrosis.

[0012] 3. Oxidative stress in radiation-induced skin damage

[0013] The pathological changes of radiation-induced skin damage are mainly changes in the epithelial germinal layer (basal cells) and blood vessels. Ionizing radiation acts on water molecules in the skin, promoting the decomposition of water, producing a large amount of ROS, causing mitochondrial dysfunction and leading to persistent oxidative stress, which ultimately seriously damages basal cells.

[0014] 4. Ozone may aggravate radiation skin damage

[0015] Since ozone can produce more ROS, this may aggravate the oxidative stress in radiation-induced skin damage, thereby exacerbating skin damage. Especially in the case where there is already a large amount of peroxide in radiation-induced skin damage, the additional oxidative stress may further damage skin cells and aggravate the damage.

[0016] Proanthocyanidins (PC) are a class of polyphenolic compounds with important biological functions, including antioxidant, anticancer, antibacterial and anti-inflammatory activities. They are used as a defense against biotic and abiotic stresses in plants, and also affect the appearance, flavor and quality of plants. Due to their strong antioxidant activity, they show significant effects in scavenging free radicals, resisting lipid peroxidation, and enhancing the activity of antioxidant enzymes, and are an effective natural antioxidant. Summary of the invention

[0017] Purpose of the invention: The purpose of the present invention is to provide a composition for preventing radiation skin damage and solve the problem of how to reduce radiation skin damage.

[0018] Technical solution: The composition for preventing radiation skin damage described in the present invention comprises independently encapsulated vegetable oil dissolved with ozone and proanthocyanidins.

[0019] The present invention utilizes the strong oxidizing effect of proanthocyanidins to effectively avoid the aggravated damage of ozone on the skin damaged parts, and gives full play to the beneficial repair effect of ozone on tissue damage. The dual antioxidant repair effect of proanthocyanidins and ozone can quickly alleviate or prevent radiation skin damage.

[0020] Preferably, the ozone content in the vegetable oil is 1-100 mg / 100 mL.

[0021] Preferably, the vegetable oil dissolved with ozone is prepared as follows:

[0022] The ozone is continuously introduced into the vegetable oil until the ozone content in the vegetable oil reaches a standard, and then the introduction of ozone is stopped to obtain the vegetable oil dissolved with ozone.

[0023] Preferably, the vegetable oil is selected from at least one of olive oil, linseed oil, grape seed oil, jojoba oil, sesame oil and zucchini seed oil.

[0024] Preferably, the proanthocyanidins are extracted from at least one of grape seeds, blueberries, blackberries, cherries, green tea, and cocoa beans.

[0025] Preferably, the mass ratio of the vegetable oil dissolved with ozone to proanthocyanidins is 1-10:0.1-2.

[0026] Another aspect of the present invention discloses the use of the composition for preventing radiation skin damage in the preparation of a drug for preventing radiation skin damage.

[0027] Beneficial effects: Compared with the prior art, the present invention has the following significant advantages:

[0028] After the ozone oil and proanthocyanidins are used in combination, the present invention has a significant alleviating effect on skin damage, and can prevent abnormal blood flow in the irradiated area. Maintaining normal blood supply in the irradiated area can effectively promote the repair of skin damage. BRIEF DESCRIPTION OF THE DRAWINGS

[0029] Figure 1 The reverse side is a diagram showing the experimental results of the present invention for alleviating and preventing radiation skin damage in mice;

[0030] Figure 2 This is a front view of the experimental results of the present invention for alleviating and preventing radiation skin damage in mice;

[0031] Figure 3 This is a diagram showing the experimental results of the present invention in maintaining normal blood supply to the irradiated area. DETAILED DESCRIPTION

[0032] The technical solution of the present invention is further described below in conjunction with the accompanying drawings.

[0033] Example 1: A composition for preventing radiation skin damage, comprising independently packaged vegetable oil dissolved with ozone and proanthocyanidin powder extracted from grape seeds, wherein the mass ratio of the vegetable oil dissolved with ozone to the proanthocyanidin is 1:0.5.

[0034] The ozone-dissolved vegetable oil is prepared as follows:

[0035] The medical ozone is continuously introduced into the olive oil until the ozone content in the olive oil reaches 30 mg / 100 mL, and then the introduction of ozone is stopped to obtain the vegetable oil dissolved with ozone.

[0036] Example 2: A composition for preventing radiation skin damage, comprising independently packaged vegetable oil dissolved with ozone and proanthocyanidin powder extracted from blueberries, wherein the mass ratio of the vegetable oil dissolved with ozone to the proanthocyanidin is 10:0.1.

[0037] The ozone-dissolved vegetable oil is prepared as follows:

[0038] Medical ozone is continuously introduced into the linseed oil until the ozone content in the linseed oil reaches 50 mg / 100 mL, and then the introduction of ozone is stopped to obtain vegetable oil dissolved with ozone.

[0039] Example 3: A composition for preventing radiation skin damage, comprising independently packaged vegetable oil dissolved with ozone and proanthocyanidin powder extracted from blackberries, wherein the mass ratio of the vegetable oil dissolved with ozone to the proanthocyanidin is 1:1.

[0040] The ozone-dissolved vegetable oil is prepared as follows:

[0041] The medical ozone is continuously introduced into the grape seed oil until the ozone content in the grape seed oil reaches 10 mg / 100 mL, and then the introduction of ozone is stopped to obtain the vegetable oil dissolved with ozone.

[0042] Embodiment 4: A composition for preventing radiation skin damage, comprising independently packaged vegetable oil dissolved with ozone and proanthocyanidin powder extracted from cherries, wherein the mass ratio of the vegetable oil dissolved with ozone to the proanthocyanidin is 5:2.

[0043] The ozone-dissolved vegetable oil is prepared as follows:

[0044] The medical ozone is continuously introduced into the sesame oil until the ozone content in the sesame oil reaches 1 mg / 100 mL, and then the introduction of ozone is stopped to obtain the vegetable oil dissolved with ozone.

[0045] Embodiment 5: A composition for preventing radiation skin damage, comprising independently packaged vegetable oil dissolved with ozone and proanthocyanidin powder extracted from green tea, wherein the mass ratio of the vegetable oil dissolved with ozone to the proanthocyanidin is 10:2.

[0046] The ozone-dissolved vegetable oil is prepared as follows:

[0047] The medical ozone is continuously introduced into the zucchini seed oil until the ozone content in the zucchini seed oil reaches 20 mg / 100 mL, and then the introduction of ozone is stopped to obtain a vegetable oil dissolved with ozone.

[0048] The composition in Example 1 was used to carry out an experiment on preventing radiation skin damage, and the method was as follows:

[0049] Construction of a mouse model of radiation skin injury: Healthy male C57BL / 6 mice were used, and the right hind limb of the mouse was irradiated once with 6mv X-rays; the irradiation range was the sole of the right hind foot; the distance from the skin was 100cm; the dose rate was 260-300MU / min; the total irradiation dose was 40Gy.

[0050] The control group was not irradiated with radiation, nor was ozone oil and proanthocyanidins administered; the 40Gy group was the model group; the 40Gy+Ozone group was treated with ozone-dissolved vegetable oil prepared in Example 1 applied to the irradiated part immediately after modeling, once every 3 days; the 40Gy+PC group was treated with proanthocyanidin powder in Example 1 in saline solution by gavage immediately after modeling, once every 3 days; the 40Gy+Ozone+PC group was treated with ozone-dissolved vegetable oil prepared in Example 1 applied to the irradiated part immediately after modeling, and the proanthocyanidin powder in Example 1 in saline solution was administered by gavage once every 3 days. The skin damage on the feet of mice in each group after radiation treatment was observed, and the results are as follows: Figure 1 and Figure 2 After the administration of ozone oil alone or proanthocyanidins alone, the improvement of skin damage in the irradiated area was not obvious, but the combined use of ozone oil and proanthocyanidins had a significant alleviating effect on the damaged skin.

[0051] Doppler speckle blood flow meter was used to detect the blood flow of the right hind foot of each group of experimental mice: the mice were placed individually in a transparent plexiglass cage, and after the mice adapted for 30 minutes, the radiation heat tester was used to irradiate the sole of the right hind foot of the mice. When the mice showed a rapid foot retraction, foot raising or foot licking reaction, the irradiation was stopped immediately, and the duration of irradiation at this time was recorded. The automatic cut-off time was 30 seconds. The measurement was repeated 5 times, with an interval of 5 minutes each time, and the average of the 5 continuous irradiation times was taken as TWL. The results are shown in Figure 3 As shown in the figure, after the combined use of ozone oil and proanthocyanidins, the blood flow in the irradiated area of ​​mice remained normal. However, the maintenance effect of blood supply in the ozone oil single administration group and proanthocyanidin single administration group was extremely limited, which further led to the weaker effect of the two on alleviating radiation skin damage.

Claims

1. A composition for preventing radiation skin damage, characterized in that: Contains individually encapsulated ozone-dissolved vegetable oils and proanthocyanidins.

2. The composition for preventing radiation skin damage according to claim 1, characterized in that: The ozone content in the vegetable oil is 1-100 mg / 100 mL.

3. The composition for preventing radiation skin damage according to claim 1, characterized in that: The ozone-dissolved vegetable oil is prepared as follows: The ozone is continuously introduced into the vegetable oil until the ozone content in the vegetable oil reaches a standard, and then the introduction of ozone is stopped to obtain the vegetable oil dissolved with ozone.

4. The composition for preventing radiation skin damage according to claim 1, characterized in that: The vegetable oil is selected from at least one of olive oil, linseed oil, grape seed oil, jojoba oil, sesame oil and zucchini seed oil.

5. The composition for preventing radiation skin damage according to claim 1, characterized in that: The proanthocyanidins are extracted from at least one of grape seeds, blueberries, blackberries, cherries, green tea and cocoa beans.

6. The composition for preventing radiation skin damage according to claim 1, characterized in that: The mass ratio of the ozone-dissolved vegetable oil to proanthocyanidins is 1-10:0.1-2.

7. Use of the composition for preventing radiation skin damage according to any one of claims 1 to 6 in the preparation of a drug for preventing radiation skin damage.

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