Microfluidic device

Abstract

The invention relates to the technical field of microfluidics, and discloses a microfluidics device. A microfluidic main body of the microfluidic device is provided with a first liquid flow channel and a second liquid flow channel, the first liquid flow channel comprises a first liquid injection port, a mixing communication port and a first pressure equalizing port which are communicated in sequence, and the second liquid flow channel comprises a second liquid injection port, a fusion cavity, a reaction cavity and a second pressure equalizing port which are communicated in sequence; the control unit comprises a micro-flow pump, a flow meter, a liquid drop generator and a temperature control chip which are in communication connection, the micro-flow pump and the flow meter are both arranged between the second liquid injection opening and the fusion cavity and used for pumping a preset amount of second solution to the fusion cavity, and the liquid drop generator is arranged at the cavity bottom of the reaction cavity and communicated with the mixing communication opening; the dispersing device is used for dispersing a first solution to be mixed with a second solution in the fusion cavity to form a mixed solution; the temperature control chip is arranged in the reaction cavity and used for providing a preset temperature for the mixed solution in the reaction cavity. The microfluidic device disclosed by the invention is higher in integration level and has more functions.

Description

Technical Field

[0001] The present invention relates to the field of microfluidic technology, and in particular to a microfluidic device. Background Art

[0002] Microfluidics is an important tool for detecting trace amounts of liquids in chemistry, biology, and medicine. The current microfluidic detection systems only reduce the structural dimensions of the flow channel, but the power source, detection unit, temperature control unit, etc. are all external. This results in low integration of microfluidic chips and large size of detection equipment.

[0003] Therefore, a microfluidic device is urgently needed to solve the above problems. Summary of the invention

[0004] Based on the above, the object of the present invention is to provide a microfluidic device with higher integration and more functions.

[0005] To achieve the above object, the present invention adopts the following technical solutions:

[0006] Microfluidic device, comprising:

[0007] The microfluidic body is provided with a first liquid flow channel and a second liquid flow channel, wherein the first liquid flow channel comprises a first liquid injection port, a mixing communication port and a first pressure equalizing port connected in sequence, and the second liquid flow channel comprises a second liquid injection port, a fusion chamber, a reaction chamber and a second pressure equalizing port connected in sequence;

[0008] A control unit is arranged in the microfluidic body, and the control unit includes a microfluidic pump, a flow meter, a droplet generator and a temperature control chip that are communicatively connected. The microfluidic pump and the flow meter are both arranged between the second injection port and the fusion chamber, and are used to pump a preset amount of the second solution into the fusion chamber. The droplet generator is arranged at the bottom of the reaction chamber and is connected to the mixing communication port, and is used to disperse the first solution to mix with the second solution in the fusion chamber to form a mixed solution; the temperature control chip is arranged in the reaction chamber, and is used to provide a preset temperature for the mixed solution in the reaction chamber.

[0009] As a preferred solution of the microfluidic device, the microfluidic body includes a seat body, the fusion chamber and the reaction chamber are both arranged on the seat body, and the seat body is also provided with a first injection channel connected to the first injection port and a first pressure equalizing channel connected to the first pressure equalizing port; the microfluidic body is also provided with a base, and the base is arranged on the side of the seat body close to the bottom of the fusion chamber, the mixing connecting port is arranged on the base, and the base is also provided with a first injection connecting port connected to the first injection channel, and a first pressure equalizing connecting port connected to the first pressure equalizing channel, and the first injection connecting port and the first pressure equalizing connecting port are both connected to the mixing connecting port.

[0010] As a preferred solution of the microfluidic device, the seat body includes a base plate, a first flow channel groove and a second flow channel groove are arranged on the side of the base plate away from the base, the reaction chamber and the second equalizing pressure port are both arranged on the base plate, one end of the first flow channel groove is connected to the reaction chamber, and the other end is provided with a fusion groove, and the second flow channel groove is connected to the fusion groove; the seat body also includes an upper cover, the upper cover is arranged on the side of the base plate away from the base, the first liquid injection port and the first equalizing pressure port are both arranged on the upper cover, and the upper cover is provided with a second liquid injection port, and the second liquid injection port can be connected to the second flow channel groove.

[0011] As a preferred solution of the microfluidic device, the base is provided with a connecting groove, and the connecting groove is connected with the second flow channel groove and the fusion cavity.

[0012] As a preferred solution of the microfluidic device, a flow detection groove is further provided on the side of the bottom plate close to the base, the flow detection groove is connected to the second flow channel groove, and the detection end of the flow meter is arranged in the flow detection groove; and / or, a micropump mounting groove is further provided on the side of the bottom plate close to the base, the micropump mounting groove is arranged at the bottom of the second flow channel groove and connected to the second flow channel groove, and the microfluidic pump is arranged in the micropump mounting groove.

[0013] As a preferred solution of the microfluidic device, the bottom plate, the upper cover and the base are all made of transparent plastic or glass; the bottom plate, the upper cover and the base are connected by bonding or bonding.

[0014] As a preferred solution of the microfluidic device, the microfluidic body also includes a lower cover, and a through groove is provided on the side of the base away from the base body, and the through groove is connected to the first liquid injection connecting port, the first pressure equalizing connecting port, and the mixing connecting port, and the lower cover is provided on the side of the base away from the base body.

[0015] As a preferred solution of the microfluidic device, the droplet generator includes a droplet generating plate and a droplet generating cable communicatively connected to the droplet generating plate, the droplet generating plate is arranged at the bottom of the fusion chamber and is connected to the mixing connecting port, and the droplet generating cable is arranged in a U shape and is clamped on the side of the lower cover and the base facing away from each other.

[0016] As a preferred solution of the microfluidic device, a chip mounting groove is further provided on the microfluidic body, the chip mounting groove is provided corresponding to the reaction chamber, and the temperature control chip is provided in the chip mounting groove.

[0017] As a preferred solution of the microfluidic device, the microfluidic pump is arranged at an end of the flowmeter away from the fusion chamber.

[0018] The beneficial effects of the present invention are:

[0019] The present invention is used to realize different functions by setting a control unit on the microfluidic body, and has a higher degree of integration. Specifically, the microfluidic body is provided with a first liquid flow channel and a second liquid flow channel, which are used for the flow of the first liquid and the second liquid respectively, wherein the injection port is used for the injection of the liquid, and the pressure equalizing port is used for the discharge of gas or liquid in the fluid channel to even out the air pressure in the liquid flow channel. Before use, the second liquid is injected from the second injection port and fills the second liquid flow channel. During the injection process, the second pressure equalizing port has gas discharge, making the injection smoother. Then the first liquid is injected from the first injection port, and during the injection process, the second pressure equalizing port has gas discharge, and then the first liquid injected by the first injection port is processed by the droplet generator, dispersed and mixed with the second solution in the fusion chamber to form a mixed solution. Next, the microfluidic pump and the flowmeter work to pump a preset amount of the second solution to the fusion chamber so that the mixed solution in the fusion chamber enters the reaction chamber, and at this time, the second liquid is discharged from the second pressure equalizing port. After the mixed solution enters the reaction chamber, the temperature control chip works to cool or heat the mixed solution in the reaction chamber to a preset temperature, thereby realizing the detection of the liquid. The above-mentioned microfluidic device has a higher degree of integration and more functions, and can be applied to different usage scenarios. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] In order to more clearly illustrate the technical solutions in the embodiments of the present invention, the following briefly introduces the drawings required for use in the description of the embodiments of the present invention. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on the contents of the embodiments of the present invention and these drawings without paying any creative work.

[0021] Figure 1 is an exploded schematic diagram of a microfluidic device provided in a specific embodiment of the present invention;

[0022] Figure 2 is a schematic diagram of a bottom plate of a microfluidic device provided in a specific embodiment of the present invention from one perspective;

[0023] Figure 3 is a schematic diagram of another perspective of the bottom plate of the microfluidic device provided in a specific embodiment of the present invention;

[0024] Figure 4 It is a schematic diagram of another viewing angle of the base of the microfluidic device provided in a specific embodiment of the present invention.

[0025] In the figure:

[0026] 110, bottom plate; 111, first injection channel; 112, first equalizing pressure channel; 113, first flow channel groove; 1131, fusion groove; 114, second flow channel groove; 115, flow detection groove; 1151, first mounting groove; 116, micro pump mounting groove; 1161, second mounting groove; 117, chip mounting groove; 118, reaction groove; 119, second equalizing pressure port; 120, upper cover; 121, first injection port; 122, first equalizing pressure port; 123, second injection port; 124, avoidance gap;

[0027] 200, base; 210, mixing communication port; 220, first liquid injection communication port; 230, first pressure equalization communication port; 240, communication groove; 250, through groove;

[0028] 310, microfluidic pump; 311, micropump cable; 320, flow meter; 321, detection end; 322, flow cable; 331, droplet generating plate; 332, droplet generating cable; 340, temperature control chip;

[0029] 410, lower cover; 420, sealing plate. DETAILED DESCRIPTION

[0030] Embodiments of the present invention are described in detail below, examples of which are shown in the accompanying drawings, wherein the same or similar reference numerals throughout represent the same or similar elements or elements having the same or similar functions. The embodiments described below with reference to the accompanying drawings are exemplary and are intended to be used to explain the present invention, and should not be construed as limiting the present invention.

[0031] In the description of the present invention, it should be noted that the terms "center", "up", "down", "left", "right", "vertical", "horizontal", "inside", "outside" and the like indicate positions or positional relationships based on the positions or positional relationships shown in the accompanying drawings, and are only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as limiting the present invention. In addition, the terms "first" and "second" are used for descriptive purposes only and cannot be understood as indicating or implying relative importance. Among them, the terms "first position" and "second position" are two different positions.

[0032] Unless otherwise clearly specified and limited, the terms "installed", "connected", "connected", and "fixed" should be understood in a broad sense, for example, it can be a fixed connection or a detachable connection; it can be a mechanical connection or an electrical connection; it can be a direct connection or an indirect connection through an intermediate medium, it can be the internal connection of two elements or the interaction relationship between two elements. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0033] In the present invention, unless otherwise clearly specified and limited, a first feature being "above" or "below" a second feature may include that the first and second features are in direct contact, or may include that the first and second features are not in direct contact but are in contact through another feature between them. Moreover, a first feature being "above", "above" and "above" a second feature includes that the first feature is directly above and obliquely above the second feature, or simply indicates that the first feature is higher in level than the second feature. A first feature being "below", "below" and "below" a second feature includes that the first feature is directly below and obliquely below the second feature, or simply indicates that the first feature is lower in level than the second feature.

[0034] The technical solution of the present invention is further described below with reference to the accompanying drawings and through specific implementation methods.

[0035] like Figure 1-Figure 4 As shown, this embodiment provides a microfluidic device, which includes a microfluidic body and a control unit arranged in the microfluidic body, the microfluidic body is provided with a first liquid flow channel and a second liquid flow channel, the first liquid flow channel includes a first injection port 121, a mixing connection port 210 and a first pressure equalizing port 122 connected in sequence, and the second liquid flow channel includes a second injection port 123, a fusion chamber, a reaction chamber and a second pressure equalizing port 119 connected in sequence; the control unit includes a microfluidic pump 310, a flow meter 320, a droplet generator and a temperature control chip 340 connected in communication, the microfluidic pump 310 and the flow meter 320 are both arranged between the second injection port 123 and the fusion chamber, and are used to pump a preset amount of the second solution into the fusion chamber, the droplet generator is arranged at the bottom of the reaction chamber, and is connected to the mixing connection port 210, and is used to disperse the first solution to mix with the second solution in the fusion chamber to form a mixed solution; the temperature control chip 340 is arranged in the reaction chamber, and is used to provide a preset temperature for the mixed solution in the reaction chamber.

[0036] By setting a control unit on the microfluidic body, it is used to realize different functions and has a higher degree of integration. Specifically, the microfluidic body is provided with a first liquid flow channel and a second liquid flow channel, which are used for the flow of the first liquid and the second liquid respectively, wherein the injection port is used for the injection of the liquid, and the pressure equalizing port is used for the discharge of gas or liquid in the fluid channel to even out the air pressure in the liquid flow channel. Before use, the second liquid is injected from the second injection port 123 and fills the second liquid flow channel. During the injection process, the second pressure equalizing port 119 has gas discharge, making the injection smoother. Then the first liquid is injected from the first injection port 121, and the second pressure equalizing port 119 has gas discharge during the injection process. Then the first liquid injected by the first injection port 121 enters the droplet generator through the mixing communication port 210, and is processed by the droplet generator, dispersed and mixed with the second solution in the fusion chamber to form a mixed solution. Next, the microfluidic pump 310 and the flowmeter 320 work to pump a preset amount of the second solution into the fusion chamber so that the mixed solution in the fusion chamber enters the reaction chamber, and the second liquid is discharged from the second pressure equalizing port 119. After the mixed solution enters the reaction chamber, the temperature control chip 340 works to cool or heat the mixed solution in the reaction chamber to a preset temperature, thereby realizing the detection of the liquid. The above-mentioned microfluidic device has a higher degree of integration and more functions, and can be applied to different usage scenarios.

[0037] It is understandable that the microfluidic device is also connected to a power supply system and a control system, wherein the power supply system is used to supply power to the control unit, and the control unit is connected to the control system in communication to operate the liquid according to a preset control program, thereby achieving a preset function. The control system and the control methods of each control unit can be set with reference to the prior art and are not specifically limited here.

[0038] Exemplarily, the above-mentioned microfluidic device can be used in dPCR (digital PCR) detection. Accordingly, the first liquid is a reaction reagent, and the second liquid is a transport liquid such as fluorinated oil. The temperature control chip 340 needs to achieve high and low temperature temperature cycles. In other embodiments, the microfluidic device can also be used in other scenarios that require temperature control and flow control, or special scenarios such as ships and space.

[0039] Specifically, the microfluidic body includes a seat body, the fusion chamber and the reaction chamber are both arranged on the seat body, and the seat body is also provided with a first injection channel 111 connected to the first injection port 121 and a first pressure equalizing channel 112 connected to the first pressure equalizing port 122; the microfluidic body is also provided with a base 200, the base 200 is arranged on the side of the seat body close to the bottom of the fusion chamber, and the mixing communication port 210 is arranged on the base 200. Correspondingly, the base 200 is also provided with a first injection communication port 220 connected to the first injection channel 111, and a first pressure equalizing communication port 230 connected to the first pressure equalizing channel 112, and the first injection communication port 220 and the first pressure equalizing communication port 230 are both connected to the mixing communication port 210. Exemplarily, the droplet generator includes a droplet generating plate 331, and the droplet generating plate 331 is arranged at the bottom of the fusion chamber to connect the mixing communication port 210 and the fusion chamber. That is, the flow path of the first liquid is as follows: the first liquid injection port 121, the first liquid injection channel 111, the first liquid injection connection port 220, and then enters the droplet generator through the mixing connection port 210. At the same time, the gas in the first liquid flow channel can be discharged from the microfluidic device through the first pressure equalization connection port 230, the first pressure equalization channel 112 and the first pressure equalization port 122. The base 200 is provided to guide the first liquid to the bottom of the fusion chamber, so that the droplet generator can disperse the first liquid into the second liquid in the fusion chamber after processing.

[0040] In this embodiment, the microfluidic body also includes a lower cover 410. A connecting groove 240 is provided on the side of the base 200 away from the base body. The connecting groove 240 is connected to the first liquid injection connecting port 220, the first pressure equalizing connecting port 230, and the mixing connecting port 210. The lower cover 410 is provided on the side of the base 200 away from the base body. The connecting groove 240 is provided to connect the first liquid injection connecting port 220, the first pressure equalizing connecting port 230, and the mixing connecting port 210. At the same time, the lower cover 410 is provided to block the connecting groove 240 to form a sealed liquid flow channel, making the processing easier. Exemplarily, the droplet generating plate 331 is provided with two liquid inlets, and correspondingly, two mixing connecting ports 210 are also provided.

[0041] Furthermore, the droplet generator includes a droplet generating cable 332 which is communicatively connected to the droplet generating plate 331. The droplet generating cable 332 is configured to be U-shaped and is clamped on the side of the lower cover 410 and the base 200 which are opposite to each other. The droplet generating cable 332 can be used to fix the lower cover 410 and the base 200, or to improve the connection reliability between the lower cover 410 and the base 200.

[0042] Exemplarily, the seat body includes a bottom plate 110, a first flow channel groove 113 and a second flow channel groove 114 are provided on a side of the bottom plate 110 away from the base 200, a reaction chamber and a second equalizing pressure port 119 are both provided on the bottom plate 110, one end of the first flow channel groove 113 is connected to the reaction chamber, and a fusion groove 1131 is provided on the other end, and the second flow channel groove 114 is connected to the fusion groove 1131; the seat body also includes an upper cover 120, and the upper cover 120 is provided on a side of the bottom plate 110 away from the base 200 to form a fusion chamber in the fusion groove 1131. A second liquid injection port 123 is provided on the upper cover 120, and the second liquid injection port 123 can be connected to the second flow channel groove 114; the first liquid injection port 121 and the first equalizing pressure port 122 are both provided on the upper cover 120, and the first liquid injection channel 111 and the first equalizing pressure channel 112 are provided on the bottom plate 110.

[0043] In this embodiment, in order to achieve the connection between the second flow channel groove 114 and the fusion groove 1131, a through groove 250 is provided on one side of the base 200 close to the bottom plate 110, and the second flow channel groove 114 and the fusion chamber are connected through the through groove 250. It can be understood that the second liquid directly enters the fusion chamber through the through groove 250 and does not pass through the droplet generating plate 331.

[0044] Optionally, a flow detection slot 115 is further provided on one side of the bottom plate 110 close to the base 200, the flow detection slot 115 is communicated with the second flow channel slot 114, the detection end 321 of the flow meter 320 is provided at the flow detection slot 115, and the flow of the liquid is measured by flowing through the detection end 321. At the same time, a micro pump installation slot 116 is further provided on one side of the bottom plate 110 close to the base 200, the micro pump installation slot 116 is provided at the bottom of the second flow channel slot 114 and communicated with the second flow channel slot 114, and the micro flow pump 310 is provided in the micro pump installation slot 116 to realize pumping liquid. In addition, the flow meter 320 is also provided with a flow cable 322, and the micro pump is also provided with a micro pump cable 311. Accordingly, the bottom plate 110 is also provided with a first mounting groove 1151 connected to the flow detection groove 115 for mounting the flow cable 322; the bottom plate 110 is also provided with a second mounting groove 1161 connected to the micro pump mounting groove 116 for mounting the micro pump cable 311; the above structures are all conducive to improving the integration of the microfluidic device. It is worth noting that the upper cover 120 is also provided with an avoidance notch 124 to avoid the installation of the micro pump cable 311 and the flow cable 322.

[0045] Preferably, the microfluidic pump 310 is disposed at an end of the flow meter 320 away from the fusion cavity. By disposing the flow meter 320 close to the fusion cavity, it is helpful to improve the accuracy of the volume of the liquid flowing into the fusion cavity, thereby improving the detection accuracy.

[0046] In this embodiment, the microfluidic body further includes a sealing plate 420, and a reaction groove 118 is provided at one end of the bottom plate 110 away from the upper cover 120. The sealing plate 420 is connected to the bottom plate 110 to block the reaction groove 118 to form a reaction chamber. Preferably, a chip mounting groove 117 is also provided on the microfluidic body, and the chip mounting groove 117 is provided corresponding to the reaction chamber, and the temperature control chip 340 is provided in the chip mounting groove 117. By making the chip mounting groove 117 correspond to the reaction chamber, the distance between the temperature control chip 340 and the reaction chamber is reduced, thereby improving the temperature control effect of the temperature control chip on the liquid in the reaction chamber.

[0047] Exemplarily, the bottom plate 110, the upper cover 120, the base 200, the lower cover 410 and the sealing plate 420 are all made of transparent plastic or glass, specifically COC. In addition, the bottom plate 110, the base 200, the lower cover 410 and the sealing plate 420 are connected by bonding or bonding, wherein the bonding can be done by glue or double-sided tape to ensure the sealing between the flow channels, and there is no specific limitation.

[0048] The above contents are only preferred embodiments of the present invention. For ordinary technicians in this field, according to the concept of the present invention, there will be changes in the specific implementation methods and application scopes. The content of this specification should not be understood as limiting the present invention.

Claims

1. A microfluidic device, characterized in that include: A microfluidic body is provided with a first liquid flow channel and a second liquid flow channel, wherein the first liquid flow channel comprises a first liquid injection port (121), a mixing communication port (210) and a first pressure equalizing port (122) which are connected in sequence, and the second liquid flow channel comprises a second liquid injection port (123), a fusion chamber, a reaction chamber and a second pressure equalizing port (119) which are connected in sequence; A control unit is arranged in the microfluidic body, and the control unit includes a microfluidic pump (310), a flow meter (320), a droplet generator and a temperature control chip (340) which are communicatively connected. The microfluidic pump (310) and the flow meter (320) are both arranged between the second injection port (123) and the fusion chamber, and are used to pump a preset amount of the second solution into the fusion chamber. The droplet generator is arranged at the bottom of the reaction chamber and is connected to the mixing communication port (210), and is used to disperse the first solution to mix with the second solution in the fusion chamber to form a mixed solution; the temperature control chip (340) is arranged in the reaction chamber, and is used to provide a preset temperature for the mixed solution in the reaction chamber.

2. The microfluidic device according to claim 1, characterized in that: The microfluidic body comprises a seat body, the fusion chamber and the reaction chamber are both arranged on the seat body, and the seat body is also provided with a first injection channel (111) connected to the first injection port (121) and a first pressure equalizing channel (112) connected to the first pressure equalizing port (122); the microfluidic body is also provided with a base (200), and the base (200) is arranged on a side of the seat body close to the bottom of the fusion chamber, and the mixing communication port (210) is arranged on the base (200), and the base (200) is also provided with a first injection communication port (220) connected to the first injection channel (111), and a first pressure equalizing communication port (230) connected to the first pressure equalizing channel (112), and the first injection communication port (220) and the first pressure equalizing communication port (230) are both connected to the mixing communication port (210).

3. The microfluidic device according to claim 2, characterized in that: The seat body comprises a bottom plate (110), a first flow channel groove (113) and a second flow channel groove (114) are arranged on a side of the bottom plate (110) away from the base (200), the reaction chamber and the second pressure equalizing port (119) are both arranged on the bottom plate (110), one end of the first flow channel groove (113) is communicated with the reaction chamber, and the other end is provided with a fusion groove (1131), and the second flow channel groove (114) is communicated with the fusion groove (1131); the seat body also comprises an upper cover (120), the upper cover (120) is arranged on a side of the bottom plate (110) away from the base (200), the first liquid injection port (121) and the first pressure equalizing port (122) are both arranged on the upper cover (120), the upper cover (120) is provided with a second liquid injection port (123), and the second liquid injection port (123) can be communicated with the second flow channel groove (114).

4. The microfluidic device according to claim 3, characterized in that: The base (200) is provided with a communication groove (240), and the communication groove (240) is in communication with the second flow channel groove (114) and the fusion cavity.

5. The microfluidic device according to claim 3, characterized in that: A flow detection groove (115) is also provided on one side of the bottom plate (110) close to the base (200), the flow detection groove (115) is communicated with the second flow channel groove (114), and the detection end (321) of the flow meter (320) is arranged in the flow detection groove (115); and / or a micro pump installation groove (116) is also provided on one side of the bottom plate (110) close to the base (200), the micro pump installation groove (116) is arranged at the bottom of the second flow channel groove (114) and is communicated with the second flow channel groove (114), and the micro flow pump (310) is arranged in the micro pump installation groove (116).

6. The microfluidic device according to claim 3, characterized in that: The bottom plate (110), the upper cover (120) and the base (200) are all made of transparent plastic or glass; the bottom plate (110), the upper cover (120) and the base (200) are connected by bonding or bonding.

7. The microfluidic device according to claim 2, characterized in that: The microfluidic body further comprises a lower cover (410); a through groove (250) is provided on a side of the base (200) away from the base body; the through groove (250) is connected to the first liquid injection connection port (220), the first pressure equalization connection port (230), and the mixing connection port (210); and the lower cover (410) is provided on a side of the base (200) away from the base body.

8. The microfluidic device according to claim 7, characterized in that: The droplet generator comprises a droplet generating plate (331) and a droplet generating cable (332) which is communicatively connected to the droplet generating plate (331); the droplet generating plate (331) is arranged at the bottom of the fusion chamber and is communicated with the mixing communication port (210); the droplet generating cable (332) is arranged in a U shape and is clamped on the side of the lower cover (410) and the base (200) which are away from each other.

9. The microfluidic device according to any one of claims 1 to 8, characterized in that: The microfluidic body is also provided with a chip mounting groove (117), the chip mounting groove (117) is arranged corresponding to the reaction chamber, and the temperature control chip (340) is arranged in the chip mounting groove (117).

10. The microfluidic device according to any one of claims 1 to 8, characterized in that: The microfluidic pump (310) is disposed at an end of the flow meter (320) away from the fusion chamber.

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