Method for improving yield of hybrid pennisetum and soil quality of planting land

Abstract

The invention discloses a method for improving the yield of hybrid pennisetum and the soil quality of a planting land, which comprises the following steps: measuring nutritional ingredients in biogas slurry of a pig farm, including organic matters, total nitrogen, total phosphorus and total potassium; biogas slurry and chemical fertilizers with different application gradients are designed to keep the nitrogen level stable; through field trials, the yield of the hybrid pennisetum and the physicochemical properties of soil under different treatments are measured so as to determine the maximum safe absorption amount. According to the method, scientific fertilization of the hybrid pennisetum is realized by accurately measuring nutritional ingredients in the biogas slurry of the pig farm and designing the biogas slurry and the chemical fertilizer with different application gradients, so that the yield of crops is remarkably increased. Meanwhile, the maximum safe absorption amount of the biogas slurry is determined through field trials, and a scientific basis is provided for reasonably utilizing livestock and poultry breeding wastes. According to the method, by optimizing application of the biogas slurry, the physical and chemical properties of soil are improved, the soil fertility is improved, resource utilization of agricultural waste is achieved, and sustainable development of ecological agriculture is promoted.

Description

Technical Field

[0001] The invention belongs to the field of agricultural biotechnology and relates to a method for improving hybrid Pennisetum yield and soil quality by rationally applying biogas slurry and chemical fertilizers. Background Art

[0002] With the growth of global population and the improvement of living standards, the demand for meat and dairy products continues to rise, which directly promotes the rapid development of livestock and poultry farming. However, while livestock and poultry farming provides a rich source of protein for humans, it also produces a large amount of waste, especially livestock and poultry manure. If these wastes are not properly handled, they will cause serious pollution to the environment and become one of the main sources of agricultural non-point source pollution.

[0003] After livestock and poultry manure is treated with anaerobic fermentation, a large amount of biogas slurry will be produced. Biogas slurry is a liquid residue rich in various nutrients, trace elements and plant growth hormones, such as gibberellins, indoleacetic acid, etc., which has the potential to improve soil fertility and soil structure. However, the imbalance of nutrient content in biogas slurry and the complex microbial community produced during anaerobic fermentation may have adverse effects on the soil environment. In particular, the inventors have found that when biogas slurry is applied in large quantities, it may lead to soil pollution and degradation, change the physical and chemical properties and fertility of the soil, and thus affect the growth and yield of crops.

[0004] There have been some studies and practices on the treatment and resource utilization of livestock and poultry waste. For example, biogas slurry is used as liquid fertilizer for irrigating crops, but due to the lack of standardized application methods, it is often difficult to control its potential negative impact on soil and crops. In addition, although studies have shown that biogas slurry can promote the growth of certain forage grasses such as perennial ryegrass and hybrid pennisetum, the application of large amounts of biogas slurry may lead to changes in soil physical and chemical properties and imbalance of soil fertility.

[0005] As a high-yield, high-quality forage grass, hybrid Pennisetum is of great significance to the sustainable development of livestock and poultry farming.

[0006] However, how to safely and efficiently utilize biogas slurry to promote the growth of hybrid Pennisetum and improve soil quality remains a technical problem that needs to be solved urgently. Summary of the invention

[0007] In view of the limitations of the prior art, the present invention aims to provide a new method to improve the yield and soil quality of hybrid Pennisetum by precisely controlling the application of biogas slurry and fertilizers, combined with the application of AMF, while reducing the risk of environmental pollution. Through this method, we can not only effectively utilize the waste generated by livestock and poultry farming, but also provide an environmentally friendly solution for sustainable agricultural development.

[0008] The inventor has continuously innovated and reformed through long-term exploration and attempts, as well as multiple experiments and efforts, to solve the above technical problems. The technical solution provided by the present invention is to provide a method for improving the yield of hybrid Pennisetum and the soil quality of the planting site, which method comprises the following steps:

[0009] Determine the nutritional content of swine farm biogas slurry, including organic matter, total nitrogen, total phosphorus and total potassium;

[0010] Design different application gradients of biogas slurry and fertilizer to keep nitrogen levels stable;

[0011] Through field experiments, the yield of hybrid Pennisetum and the physical and chemical properties of soil under different treatments were measured to determine the maximum safe absorption capacity.

[0012] Compared with the prior art, the present invention has the following beneficial effects:

[0013] This technical solution achieves scientific fertilization of hybrid Pennisetum by accurately measuring the nutrient components in the biogas slurry in the pig farm and designing biogas slurry and fertilizers with different application gradients, thereby significantly increasing the yield of crops. At the same time, through field experiments, the yield of hybrid Pennisetum and the physical and chemical properties of soil under different treatments were measured, and the maximum safe absorption capacity of biogas slurry was effectively determined, providing a scientific basis for the rational use of livestock and poultry breeding waste. This method not only improves the yield and quality of feed crops and increases agricultural economic benefits, but also improves the physical and chemical properties of the soil and improves soil fertility by optimizing the application of biogas slurry, realizes the resource utilization of agricultural waste, and promotes the sustainable development of ecological agriculture. In addition, by controlling the application amount of biogas slurry within a safe range, the environmental pollution and ecological risks that may be caused by excessive application are reduced, and the safety of the agricultural ecological environment is guaranteed. Therefore, this technical solution has multiple beneficial effects of increasing crop yields, optimizing soil quality, resource utilization of agricultural waste, and protecting the agricultural ecological environment.

[0014] On the basis of the above technical solution, the present invention can also be improved as follows:

[0015] Furthermore, the total nitrogen application rate of the biogas slurry and fertilizer is 275 kg / hm 2 .

[0016] Compared with the prior art, the beneficial effects of adopting the above further technical solution are:

[0017] By precisely controlling the amount of nitrogen applied, the growth of hybrid Pennisetum can be more effectively promoted, its biomass and yield can be increased, thereby increasing the feed supply for animal husbandry. This precise fertilization method helps avoid environmental pollution caused by excessive nitrogen fertilizer application, such as eutrophication of water bodies caused by nitrogen loss, thereby protecting the agricultural ecological environment. In addition, this fertilization strategy can also help improve fertilizer utilization efficiency, reduce agricultural production costs, and promote sustainable agricultural development.

[0018] On the basis of the above technical solution, the present invention can also be improved as follows:

[0019] Furthermore, the biogas slurry contains 937.71 mg / L of organic matter, 228.50 mg / L of total nitrogen, 46.57 mg / L of total phosphorus and 101.48 mg / L of total potassium, and the application amount is 800 tons / hectare.

[0020] Compared with the prior art, the beneficial effects of adopting the above further technical solution are:

[0021] This further technical solution accurately meets the nutrients required for the growth of hybrid Pennisetum, achieving precise fertilization. This improvement not only improves the utilization efficiency of fertilizers and reduces the risk of environmental pollution caused by excessive fertilization, such as reducing the loss of nitrogen, phosphorus, potassium and other elements in water bodies, thereby reducing the impact of eutrophication on water bodies, but also helps to improve crop yields and quality. In addition, by optimizing the application amount of biogas slurry, the soil structure and biological activity can be better improved, the long-term fertility of the soil can be enhanced, and the sustainable development of agriculture can be promoted.

[0022] On the basis of the above technical solution, the present invention can also be improved as follows:

[0023] According to one embodiment of the method of the present invention, the urea application rate is 180 kg / hectare, the superphosphate application rate is 461 kg / hectare, and the potassium chloride application rate is 160 kg / hectare.

[0024] Compared with the prior art, the beneficial effects of adopting the above further technical solution are:

[0025] By adopting the above further technical solution, the amount of fertilizer applied is significantly reduced, that is, the amount of urea is reduced from 598 kg / ha to 180 kg / ha, the amount of superphosphate is reduced from 1528 kg / ha to 461 kg / ha, and the amount of potassium chloride is reduced from 529 kg / ha to 160 kg / ha, which is a significant reduction in the amount of fertilizer used compared with the prior art. This improvement not only reduces agricultural production costs and improves economic benefits, but more importantly, it greatly reduces the potential negative impact of excessive fertilizers on the environment, such as reducing water eutrophication and soil structure damage caused by fertilizer loss, thereby helping to protect the agricultural ecological environment and improve the sustainable production capacity of the soil. This precision fertilization method also helps to improve the efficiency of fertilizer absorption by crops, promote healthy crop growth, and increase crop yield and quality.

[0026] On the basis of the above technical solution, the present invention can also be improved as follows:

[0027] According to one embodiment of the method of the present invention, the biogas slurry and chemical fertilizer are applied four times a year, and biogas slurry irrigation and fertilizer application are carried out during the greening stage, jointing stage, booting stage and filling stage of hybrid Pennisetum.

[0028] Compared with the prior art, the beneficial effects of adopting the above further technical solution are:

[0029] By providing the required nutrients at the critical stage of crop growth, the growth and development of hybrid Pennisetum can be more effectively promoted, thereby increasing crop yield and biomass. The phased fertilization strategy helps to improve the utilization efficiency of fertilizers and reduce nutrient losses, especially key nutrients such as nitrogen, phosphorus, and potassium. This not only reduces agricultural production costs, but also reduces the burden that excessive fertilization may cause on the environment, such as reducing eutrophication of water bodies and soil degradation caused by nutrient loss. This precision fertilization method also helps to balance the nutrient supply in the soil, maintain soil health and productivity, and promote sustainable agricultural development.

[0030] On the basis of the above technical solution, the present invention can also be improved as follows:

[0031] According to one embodiment of the method of the present invention, the method further comprises the following steps:

[0032] Adding arbuscular mycorrhizal fungi spore matrix to the surface soil of hybrid Pennisetum planting areas can improve yield.

[0033] Preferably, the arbuscular mycorrhizal fungus spore matrix is ​​Claroideoglomus etunicatum, containing 15 to 20 spores per gram.

[0034] By adopting the above-mentioned further technical solution, adding arbuscular mycorrhizal fungi (AMF) spore matrix to the surface soil of hybrid Pennisetum planting area can significantly improve the yield and soil quality of hybrid Pennisetum compared with the existing technology. AMF forms a symbiotic relationship with the root system of hybrid Pennisetum, and through its extensive hyphae network, increases the absorption area of ​​hybrid Pennisetum for water and nutrients, and improves the nutrient absorption efficiency of hybrid Pennisetum, especially the absorption of phosphorus, which is crucial for plant growth. In addition, the addition of AMF can also enhance the stress resistance of plants and improve their resistance to adverse environmental conditions such as drought and disease. At the soil level, the activity of AMF can improve soil structure, increase soil aggregation, and improve soil water holding capacity and aeration, thereby promoting the diversity and activity of soil microbial communities and enhancing soil biological fertility.

[0035] Furthermore, the hybrid Pennisetum is planted by single stem node cuttings, with a plant spacing of 80 cm and a row spacing of 1 m.

[0036] The present invention also provides a system for treating and recycling livestock and poultry breeding waste, comprising:

[0037] a. A facility for collecting and storing swine biogas;

[0038] b. an analytical device for determining the nutrient content of biogas slurry;

[0039] c. A field application device for applying biogas slurry and fertilizer;

[0040] d. A monitoring system for monitoring hybrid Pennisetum growth and soil quality changes.

[0041] Furthermore, a device for adding AMF spore substrate is included.

[0042] Compared with the prior art, the present invention has the following beneficial effects:

[0043] Through integrated facilities and equipment, the system realizes the whole process management from waste collection, nutrient analysis, precision fertilization to growth monitoring, improving the efficiency of waste treatment and the scientific nature of resource utilization. By measuring the nutrient composition of biogas slurry and carrying out precise fertilization, it not only improves the utilization efficiency of fertilizers and reduces the potential impact of excessive fertilization on the environment, but also helps to improve the yield and quality of crops. The monitoring function of the system can track the changes in crop growth and soil quality in real time, provide scientific data support for agricultural production, and guide the precision management of agricultural production. Furthermore, by adding AMF spore matrix, the nutrient absorption capacity and stress resistance of crops can be further improved, the diversity of soil microorganisms can be promoted, and the biological fertility of the soil can be enhanced. BRIEF DESCRIPTION OF THE DRAWINGS

[0044] In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings required for use in the embodiments will be briefly introduced below. It should be understood that the following drawings only show certain embodiments of the present invention and therefore should not be regarded as limiting the scope. For ordinary technicians in this field, other related drawings can be obtained based on these drawings without paying creative work.

[0045] Figure 1 It is the distribution diagram of each treatment in the field test design.

[0046] Figure 2 Figure 2 shows the effects of different biogas slurry application rates on hybrid Pennisetum yield in different periods. Figure a is June 2023, Figure b is August 2023, and Figure c is October 2023. Different lowercase letters in the figures indicate significant differences between different treatments (P < 0.05).

[0047] Figure 3 The figure shows the effects of different biogas slurry dosages on the contents of protein (Figure a), crude fat (Figure b), crude fiber (Figure c), nitrogen-free extract (Figure d) and crude ash (Figure e) in hybrid Pennisetum. Different lowercase letters in the figure indicate significant differences among different treatments (P < 0.05).

[0048] Figure 4 Effects of different biogas slurry dosages on soil pH. Different lowercase letters in the figure indicate significant differences among different treatments (P<0.05).

[0049] Figure 5 Effects of different biogas slurry dosages on the contents of soil total nitrogen (Figure a), nitrate nitrogen (Figure b), ammonia nitrogen (Figure c), organic matter (Figure d), available phosphorus (Figure e), and available potassium (Figure f). Different lowercase letters in the figures indicate significant differences among different treatments (P < 0.05). DETAILED DESCRIPTION

[0050] The following describes the invention in conjunction with specific embodiments.

[0051] In order to make the purpose, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below in combination with the embodiments of the present invention. Obviously, the described embodiments are part of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention. Therefore, the following detailed description of the embodiments of the present invention is not intended to limit the scope of the invention claimed for protection, but merely represents selected embodiments of the present invention.

[0052] In the present invention, unless otherwise specified, all equipment and raw materials can be purchased from the market or are commonly used in the industry. The methods in the following embodiments, unless otherwise specified, are conventional methods in the art.

[0053] This experiment was conducted at the Shuanghe base of Chongqing Academy of Animal Husbandry Sciences. Reyan No. 4 hybrid Pennis purpureum×P.tyhoideum cv.Reyan No.4 was selected as the test material. The nitrogen content of the biogas slurry was 0.23 g / L (Table 1). The optimal nitrogen requirement of hybrid Pennis purpureum was determined to be 275 kg / hm 2 The following experimental treatments were designed accordingly, with the maximum biogas slurry application rate being 1200 t / ha (Table 3).

[0054] Table 1: Main components of biogas slurry from pig farms near the experimental site

[0055]

[0056] Table 2: Physical and chemical properties of soil in the experimental site

[0057]

[0058] Table 3: Design of biogas slurry and fertilizer treatment in this experiment

[0059]

[0060] In Table 3, the total amount of biogas slurry treated by T4 is 800 tons / hectare, and that of T6 is 1200 tons / hectare.

[0061] The experiment was conducted in 7 treatments, each with 3 replicates. T stands for treatment and R stands for replicate. There were 1 fertilizer treatment and 6 biogas slurry gradient treatments. Each treatment had 3 replicates, for a total of 21 plots (see Figure 1 The plot is 5m long and 3m wide. Hybrid Pennisetum is planted by single stem node cuttings. There are 20 plants in each plot, with a spacing of 80cm between plants and 1m between rows.

[0062] In March, May, July and September every year, hybrid Pennisetum is irrigated with biogas slurry and fertilized. Among them, three kinds of fertilizers are used, namely nitrogen fertilizer, phosphorus fertilizer and potassium fertilizer. The application amount of phosphorus fertilizer and potassium fertilizer is equal to that of nitrogen fertilizer. The plants are mowed and the yield is measured in June, August and October every year. The morphological indicators of hybrid Pennisetum are measured for each yield measurement. After the yield measurement, a certain amount of hybrid Pennisetum is randomly taken to measure the water content, and the nutritional quality of hybrid Pennisetum is measured. The five-point method is used to collect soil from 0-20 soil layers in each plot for measurement of soil physical and chemical properties.

[0063] Index determination

[0064] The agronomic traits of hybrid Pennisetum were measured in a June 2023, b August 2023, and c October 2023, respectively.

[0065] Plant height: Use a ruler to measure the plant height (the height from the base of the main stem to the top of the leaves on the upper part of the plant).

[0066] Leaf length: the length of the leaf corresponding to the second node from the top.

[0067] Leaf width: The widest width of the leaf corresponding to the second node from the top.

[0068] Stem diameter: the diameter of the second node of the above-ground part, calculate the average value.

[0069] The measurement results are shown in Table 4.

[0070] Number of tillers: Take 5 representative plants from each plot, count their tiller numbers, and then take the average value.

[0071] Number of stem nodes: Five representative plants were selected from each plot, and the number of stem nodes of their above-ground parts was counted.

[0072] The measurement results are shown in Table 5.

[0073] Table 4 Measurement results of plant height, leaf length, leaf width and stem diameter

[0074]

[0075]

[0076] Note: Lowercase letters in the table indicate significant differences between different treatments for the same index (P < 0.05) Table 5 Determination results of tiller number and stem node number

[0077]

[0078]

[0079] The results of the effects of different biogas slurry application rates on the yield of hybrid Pennisetum at different stages are as follows: Figure 2 shown. Figure 2 In the figure, Figure a shows the yield measurement results in June 2023, Figure b shows the yield measurement results in August 2023, and Figure c shows the yield measurement results in October 2023. Different lowercase letters in the figures indicate significant differences between different treatments (P < 0.05).

[0080] According to the yield data, hybrid Pennisetum had the highest yield in August, and its agronomic traits such as plant height were higher than those in the other two yield measurements. The plant height, leaf length and leaf width of hybrid Pennisetum were greatly affected by the amount of biogas slurry applied, and showed a trend of first increasing and then decreasing with the increase of biogas slurry application. In addition, the application of biogas slurry significantly increased the number of tillers of hybrid Pennisetum. The yield of hybrid Pennisetum first increased and then decreased with the increase of biogas slurry application. The total yield of T2 and T4 reached 85742kg / hm 2 and 85178kg / hm 2 The application of biogas slurry significantly increased the protein content of hybrid Pennisetum, reduced the cellulose content of the plant, and improved the nutritional quality and palatability of hybrid Pennisetum.

[0081] Determination of nutritional components of hybrid Pennisetum

[0082] After the yield measurement, three hybrid Pennisetum plants were randomly selected from each plot, and their fresh weight was recorded. Then they were dried in an oven until constant weight was obtained, and their dry weight was recorded to obtain their water content. The remaining dry samples were used to measure nutritional quality:

[0083] Coarse ash (Ash): ash gravimetric method,

[0084] Crude fat (Ether extracts, EE): acid hydrolysis,

[0085] Crude protein (CP): Kjeldahl method,

[0086] Crude fiber (CF): acid-base digestion method,

[0087] Nitrogen free extract (NFE): subtraction method.

[0088] Effect of different biogas slurry dosage on protein content of hybrid Pennisetum Figure 3 a), crude fat ( Figure 3 b), crude fiber ( Figure 3 c), nitrogen-free extract ( Figure 3 d), crude ash ( Figure 3 Different lowercase letters in the figure indicate significant differences among different treatments (P<0.05).

[0089] Soil physical and chemical properties determination

[0090] Soil pH: Potentiometric method: Accurately weigh 10 g of air-dried soil, add 25 mL of distilled water, stir for 1-2 min, let stand for 30 min, and measure with a Delta 320 pH meter.

[0091] Effects of different biogas slurry dosages on soil pH Figure 4 Different lowercase letters in the figure indicate significant differences among different treatments (P<0.05).

[0092] Total nitrogen (TN): Kjeldahl method. Accurately weigh 1.0 g of air-dried soil sample, place it at the bottom of a digestion tube, add 10 mL of 98% sulfuric acid and 5 g of catalyst (K 2 SO 4 ∶CuSO 4 ·5H 2 O=500∶50), boiled at 42℃ for about 2h. After cooling, the soil total nitrogen analysis was performed using a fully automatic Kjeldahl nitrogen analyzer.

[0093] Organic matter (SOM): Potassium dichromate volumetric method-external heating method. Accurately weigh 0.5g of soil sample into a hard large test tube, add 0.1g of powdered silver sulfate, and then accurately add 5mL of 0.8mol / L (1 / 6K2Cr2O7) solution, concentrated H 2 SO 4 5 mL, carefully rotate and shake. After boiling at 210℃ for 10 minutes in a digestion furnace, wash the solution into a 250mL conical flask, making the volume in the flask about 60-80mL, add 3-4 drops of o-phenanthroline indicator, and titrate with 0.2mol / L ferrous sulfate. The end point is when the solution changes from orange to blue-green to brown-red. Record the amount of ferrous sulfate used.

[0094] Nitrate nitrogen (NO 3 - -N): Phenol disulfonic acid colorimetric method. Weigh 50g of fresh soil sample and place it in a 500mL conical flask, add 0.5g of calcium sulfate and 250mL of distilled water, and shake it with a shaker for 10 minutes. Take it out and filter it, take 25mL of the filtrate in an evaporating dish, add calcium carbonate, evaporate it in a water bath until it is dry, then add phenol disulfonic acid reagent, water and 1:1 ammonia water (excess), mix well, and measure its absorbance at a wavelength of 420nm.

[0095] Ammonium nitrogen (NH 4 + -N): KCl extraction-indophenol blue colorimetric method. Weigh 10g of fresh soil sample, place in a 250mL conical flask, add 50mL of potassium chloride solution, and shake on a shaker for 1h. Take out and filter, take 5mL of filtrate, add phenol solution, sodium chloride alkaline solution and masking agent in sequence, mix well, and measure its absorbance at a wavelength of 625nm.

[0096] Available phosphorus (AP): 1 / 2H 2 SO4 Method. Accurately weigh 5.00 g of air-dried soil sample, put it into a 50 mL conical flask, add the extractant (accurately measure 4 mL of concentrated HCL and concentrated H 2 SO 4 0.7mL, put into a 1L volumetric flask, add water to make up to 25mL, shake on a shaker for 5 minutes, and filter. Take 1mL of the filtrate, add 24mL of the working solution, shake well, and after leaving for 0.5h, perform colorimetric determination on a spectrophotometer at a wavelength of 700nm (use the extractant blank to adjust to zero).

[0097] Available potassium (AK): ammonium acetate extraction-flame photometry. Accurately weigh 5.00 g of air-dried soil, put it into a 50 mL conical flask, add 50 mL of soil extract (1 mol / L ammonium acetate, pH 7.0), shake it thoroughly for 30 minutes, take it out and filter it, and the filtrate is directly measured by flame photometer.

[0098] Effects of different biogas slurry dosages on soil total nitrogen ( Figure 5 a), nitrate nitrogen ( Figure 5 b), ammonia nitrogen ( Figure 5 c), organic matter ( Figure 5 d), fast-acting phosphorus ( Figure 5 Medium e), fast-acting potassium ( Figure 5 f) content, and different lowercase letters in the figure indicate significant differences among different treatments (P < 0.05).

[0099] The present invention effectively adjusts the pH value of the soil by scientifically applying biogas slurry, prevents the soil acidification problem caused by the single application of compound fertilizer, and thus maintains the healthy state of the soil.

[0100] As the amount of biogas slurry applied increases, the content of ammonia nitrogen and available phosphorus in the soil increases significantly, which helps to improve soil fertility and the nutrient supply of crops.

[0101] At the same time, the organic matter and total nitrogen content in the soil decreased at the beginning of the biogas slurry application, and then increased with the increase of the application amount, indicating that the application of biogas slurry helps the circulation and replenishment of soil nutrients. However, when the biogas slurry application amount exceeds a certain limit (as observed in the T5 and T6 treatment groups), the total nitrogen content in the soil continues to increase, but the crop yield decreases, indicating that the soil's absorption capacity has reached its limit.

[0102] Based on this, this study determined that the maximum safe absorption capacity of biogas slurry in hybrid foxtail grassland is 800 tons / hectare. This finding is of great significance for avoiding soil environmental damage and pollution caused by excessive application of biogas slurry.

[0103] Therefore, the present invention not only improves soil productivity, but also ensures the sustainability of the soil environment, and provides an environmentally friendly and efficient solution for the treatment and resource utilization of livestock and poultry breeding waste.

[0104] In addition to the field experiment, the inventors also conducted a pot experiment: no fertilizer was added, only biogas slurry was used, and gradient treatment was used to explore the effect of arbuscular mycorrhizal fungi (AMF) on the absorption of biogas slurry by hybrid Pennisetum.

[0105] In the AMF treatment group, 200 g (15-20 spores per g) of AMF (Claroideoglomus etunicatum, purchased from the National Center for Microbiological Resources) zeolite medium was added to the humus soil matrix in each pot (35 cm in diameter and 38 cm in depth), and the same amount of soil matrix was added to the CK group. In this study, hybrid Pennisetum (Guiminyin) was used as the plant material, provided by Sichuan Agricultural University, and propagated by stem node cuttings. After 3 months of cultivation, plants with consistent growth were selected as transplant test objects, and 3 hybrid Pennisetum seedlings were planted in each pot. The biogas slurry treatment method was consistent with the field (biogas slurry application amount and chemical fertilizer application amount). Seven different biogas slurry application levels were set for the AMF group and the CK group, and biogas slurry was applied once every other month. Finally, the yield of hybrid Pennisetum was measured.

[0106] Table 6 Biogas slurry treatment design, 7 treatments in total; biogas slurry was applied 3 times

[0107]

[0108] Table 7 Effects of AMF treatment and different biogas slurry application rates on hybrid Pennisetum yield (kg / plant)

[0109]

[0110] Note: Different lowercase letters indicate significant differences between the presence and absence of arbuscular mycorrhizal fungi in the same biogas slurry treatment (P<0.05).

[0111] The yield results show that adding a substrate containing AMF spores to the hybrid Pennisetum soil can significantly increase the yield of hybrid Pennisetum. As one of the AMF, Claroideoglomus etunicatum can significantly improve the plant's absorption efficiency of nutrients such as phosphorus and nitrogen, thereby promoting the growth of hybrid Pennisetum. The hyphae network of Claroideoglomus etunicatum helps to improve the soil's aggregation structure, increase soil water retention and air permeability, and thus promote soil health; the symbiotic relationship between the fungus and hybrid Pennisetum can promote the growth of hybrid Pennisetum roots, increase the surface area of ​​the roots, and further improve the hybrid Pennisetum's ability to absorb nutrients. In short, the application of AMF promotes the absorption of water and nutrients by enhancing the interaction between plant roots and soil.

[0112] In the description of the present invention, it should be understood that "-" and "~" represent the range between two values, and the range includes the endpoints. For example: "AB" represents a range greater than or equal to A and less than or equal to B. "A~B" represents a range greater than or equal to A and less than or equal to B.

[0113] In the description of the present invention, the term "and / or" herein is merely a description of the association relationship of associated objects, indicating that three relationships may exist. For example, A and / or B may represent three situations: A exists alone, A and B exist at the same time, and B exists alone.

[0114] In the description of the invention, the numerical values ​​of time, temperature, ratio, mass, etc. involved may be based on actual measurements, standard parameters of equipment, simplified rounding results, or within an acceptable error range, ensuring the practicability and repeatability of the invention.

[0115] In the description of the present invention, the term "about" or "approximately" is used to express the approximate value of a numerical value or range, allowing a certain error to ensure the flexibility and practicality of the description while remaining within an acceptable error range, with the maximum error range not exceeding 10% of the corresponding numerical value or numerical range.

[0116] The above are only preferred embodiments of the present invention. It should be noted that the above preferred embodiments should not be regarded as limiting the present invention, and the protection scope of the present invention should be based on the scope defined by the claims. For ordinary technicians in this technical field, several improvements and modifications can be made without departing from the spirit and scope of the present invention, and these improvements and modifications should also be regarded as the protection scope of the present invention.

Claims

1. A method for improving the yield of hybrid Pennisetum and the soil quality of the planting site, characterized in that: The method comprises the following steps: Determine the nutritional content of swine farm biogas slurry, including organic matter, total nitrogen, total phosphorus and total potassium; Design different application gradients of biogas slurry and fertilizer to keep nitrogen levels stable; Through field experiments, the yield of hybrid Pennisetum and the physical and chemical properties of soil under different treatments were measured to determine the maximum safe absorption capacity.

2. The method for improving hybrid Pennisetum yield and soil quality of planting sites according to claim 1, characterized in that: The total nitrogen application rate of biogas slurry and fertilizer is 275kg / hm 2 .

3. The method for improving hybrid Pennisetum yield and soil quality of planting sites according to claim 1, characterized in that: The biogas slurry contains 937.71 mg / L organic matter, 228.50 mg / L total nitrogen, 46.57 mg / L total phosphorus and 101.48 mg / L total potassium, and the application amount is 800 tons / hectare.

4. The method for improving hybrid Pennisetum yield and soil quality of planting sites according to claim 1, characterized in that: The urea application rate is 180 kg / hectare, the superphosphate application rate is 461 kg / hectare, and the potassium chloride application rate is 160 kg / hectare.

5. The method for improving the yield of hybrid Pennisetum and the soil quality of the planting site according to any one of claims 1 to 4, characterized in that: The biogas slurry and chemical fertilizers are applied four times a year, and the biogas slurry irrigation and fertilizer application are respectively carried out during the greening stage, jointing stage, booting stage and grain filling stage of hybrid Pennisetum.

6. The method for improving hybrid Pennisetum yield and soil quality of planting sites according to claim 1, characterized in that: The following steps are also included: Adding arbuscular mycorrhizal fungi spore matrix to the surface soil of hybrid Pennisetum planting areas can improve yield.

7. The method for improving the yield of hybrid Pennisetum and the soil quality of the planting site according to claim 6, characterized in that: The arbuscular mycorrhizal fungus spore matrix is ​​Claroideoglomus etunicatum, and each gram contains 15 to 20 spores.

8. The method for improving hybrid Pennisetum yield and soil quality of planting sites according to claim 1, characterized in that: The hybrid Pennisetum is planted by single stem node cuttings, with a plant spacing of 80 cm and a row spacing of 1 m.

9. A system for treating and recycling livestock and poultry breeding waste, characterized in that: include a. A facility for collecting and storing swine biogas; b. an analytical device for determining the nutrient content of biogas slurry; c. A field application device for applying biogas slurry and fertilizer; d. A monitoring system for monitoring hybrid Pennisetum growth and soil quality changes.

10. The system according to claim 9, characterized in that Also included is a device for adding AMF spore substrate.

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