Organ-like cryopreservation cooling box

Abstract

The invention discloses an organoid cryopreservation cooling box, and belongs to the technical field of organoid cryopreservation. The organ cryopreservation cooling box comprises a box body, the top face of the box body is a conical face, side cabins are arranged on the conical face in the length direction of a plurality of generatrixes of the conical face, the bottom faces of the side cabins are inclined faces parallel to the notch direction, and isopropanol is arranged at the bottoms of the side cabins; the organ cryopreservation cooling box further comprises a plurality of cryopreservation tube carriers, each side cabin corresponds to the multiple cryopreservation tube carriers, and each cryopreservation tube carrier comprises a sliding piece and two elastic arc-shaped pieces. The sliding piece strides across the notches of the side cabins and slides on the notches of the side cabins. The two elastic arc-shaped pieces are arranged in the side cabins, and the ends of the two arc-shaped pieces are fixedly connected with the middle of the sliding piece. The organoid cryopreservation cooling box can adapt to cryopreservation tubes of different specifications, the utilization efficiency of the cryopreservation box is improved, universality is high, and the requirement for cryopreservation of various organoid cells of different tissues in the same organoid cryopreservation box can be met.

Description

Technical Field

[0001] The present invention relates to the technical field of organoid cryopreservation, and in particular to an organoid cryopreservation cooling box. Background Art

[0002] The organoid cryopreservation box is mainly due to the urgent need for organoid preservation technology in modern biomedical research. As a three-dimensional cell culture, organoids can simulate some structures and functions of real organs, providing valuable experimental models for disease research, drug screening and regenerative medicine.

[0003] However, the existing organoid cryopreservation cooling box needs to be added with isopropanol, which is used as the main carrier for heat storage and dissipation. After the heat is dissipated from the surface or tiny gaps of the cryopreservation box, it is first quickly dissipated from the isopropanol through convection and conduction, and then the heat in the cryopreservation tube is transferred to the outside through the isopropanol, so that each cryopreservation tube in the organoid cryopreservation cooling box is gradually cooled at a relatively consistent rate, achieving a cooling effect of -1ºC / min.

[0004] Although the existing organoid cryopreservation boxes meet general experimental requirements, the experimental efficiency is relatively low. In the daily operation of organoid cryopreservation experiments, the diameters and lengths of cryopreservation tubes used for organoid cells of different tissues are often different, and they cannot be cryopreserved uniformly in the same organoid cryopreservation box, which leads to low utilization efficiency of cryopreservation boxes. Multiple organoid cryopreservation boxes are often required for classified cryopreservation to achieve the experimental purpose. Therefore, each existing organoid cryopreservation cooling box can only adapt to one specification of cryopreservation tubes, which has poor versatility and cannot meet the needs of cryopreserving organoid cells of multiple different tissues in the same organoid cryopreservation box. Summary of the invention

[0005] The purpose of the present invention is to overcome the problems in the prior art. The provided organoid cryopreservation cooling box can adapt to cryopreservation tubes of different specifications, thereby improving the utilization efficiency of the cryopreservation box. The box has high versatility and can meet the needs of cryopreserving organoid cells of various different tissues in the same organoid cryopreservation box.

[0006] The present invention provides an organoid cryopreservation cooling box including a box body, wherein the top surface of the box body is a conical surface, and side chambers are arranged on the conical surface along the length direction of multiple busbars of the conical surface, and the bottom surface of the side chamber is an inclined surface parallel to the direction of the notch, and isopropanol is provided at the bottom of the side chamber; the organoid cryopreservation cooling box also includes multiple cryopreservation tube carriers, each of the side chambers corresponds to multiple cryopreservation tube carriers, and each cryopreservation tube carrier includes: a sliding member and two elastic arc-shaped members; the sliding member crosses the notch of the side chamber and slides on the notch of the side chamber; the two elastic arc-shaped members are both arranged in the side chamber, and one end of the two arc-shaped members is fixedly connected to the middle part of the sliding member, and the other ends are staggered with each other for clamping the cryopreservation tubes.

[0007] Preferably, each of the side chambers is provided with a first sliding hole parallel to the length direction of the slot on both sides, and the side of the first sliding hole is connected to the side of the side chamber; the sliding member is a sliding rod, the sliding rod is located in the side chamber, and the two ends of the sliding rod extend into the first sliding holes on both sides of the side chamber, and the two ends of the sliding rod are fixedly connected with a sliding block, and the sliding block is slidably connected in the first sliding hole.

[0008] Preferably, the inner arc surfaces of the two arc-shaped members are arranged opposite to each other, and the two arc-shaped members are staggered to form an intersection point. An elastic pad is fixedly provided on the inner arc surface of each arc-shaped member, and a limiting column for blocking the other arc-shaped member is provided on one arc-shaped member in each freezing tube carrier, and the limiting column is located on the side of the intersection point away from the sliding member.

[0009] Preferably, a compensation chamber is provided in the box body, and the compensation chamber is located below the side chamber. The side chamber and the compensation chamber are separated by a layer plate, and a liquid outlet is provided in the layer plate. An extrusion unit is provided at the bottom of the compensation chamber for squeezing the isopropyl alcohol in the compensation chamber into the side chamber through the liquid outlet.

[0010] Preferably, there are multiple liquid outlets on the bottom surface of the side chamber, and the multiple liquid outlets are evenly arranged along the length direction of the bottom surface of the side chamber, and the number of liquid outlets is the same as the number of the freezing tube carriers; the sliding member is fixedly connected to a partition, the bottom of the partition is fixedly connected to an inclined plate, the surface of the inclined plate is slidably connected to the bottom surface of the side chamber, and the inclined plate is provided with liquid guide holes. The number of liquid outlets is the same as the number of liquid guide holes. After the sliding member moves, one liquid outlet can be connected to one liquid guide hole, which facilitates isopropyl alcohol to enter the side chamber between the multiple partitions from the compensation chamber.

[0011] Preferably, a plurality of vertical holes are provided on the top of the central chamber, and a valve unit is correspondingly arranged in each vertical hole, the number of the vertical holes, the number of the side chambers and the number of the valve units are all the same, and each valve unit comprises: a vertical rod, a first spring and a plug; the vertical rod is passed through the vertical hole; the first spring is sleeved on the vertical rod, and one end is connected to the vertical rod, and the other end is connected to the top surface of the central chamber, and the first spring is used to reset the vertical rod; the plug is fixedly connected to the lower end of the vertical rod, and is used to block the liquid inlet provided on the side wall of the side chamber, and the liquid inlet is opened by moving the upper end of the vertical rod, and the vertical rod drives the plug to move.

[0012] Preferably, a molecular sieve is provided inside the central compartment, and the upper and lower ends of the molecular sieve are fixedly connected to the box body. The lower end of the molecular sieve is provided with a plug port connected to the interior of the molecular sieve, and a plug body is provided in the plug port. The inner wall of the plug port is connected to one end of multiple conduits, and the other end of each conduit is connected to one of the compensation chambers.

[0013] Preferably, the extrusion unit includes: an extrusion plate, a sleeve and a second spring; the surface of the extrusion plate abuts against the lower surface of the inner part of the compensation chamber, and an active cavity is provided below the compensation chamber; the sleeve is fixedly connected to the extrusion plate and passes through a through hole provided at the bottom of the compensation chamber to enter the active cavity; the second spring is located in the sleeve, and its two ends respectively abut against the surface of the extrusion plate and the inner wall of the active cavity, so as to push the extrusion plate upward.

[0014] Preferably, a second sliding hole is provided at the end of the extrusion plate close to the catheter, and an extension rod is respectively connected to the extrusion plate on both sides of the second sliding hole, and the two extension rods are fixedly connected to the two ends of the connecting rod at the bottom of the side away from the extrusion plate, and the connecting rod is fixedly connected to one end of the push rod on the side away from the extrusion plate, and the other end of the push rod abuts the plug; a third sliding hole is provided on the bottom wall of the movable cavity, and the third sliding hole is slidably connected to the extension rod, and the space between the two extension rods forms a fourth sliding hole, and a sealing plate is slidably connected in the fourth sliding hole, and the sealing plate is fixedly arranged in the box body, and the sealing plate is used to ensure the sealing effect during the up and down movement of the extrusion plate, and the catheter passes through the sealing plate into the compensation chamber.

[0015] Preferably, the inner wall of the plug mouth is provided with an internal thread, the plug body is provided with an external thread, the plug mouth cooperates with the plug body thread, and the end face of the plug body close to the molecular sieve is provided with a accommodating cavity, which is screwed into the plug body, the plug body blocks the conduit, and pushes isopropanol into the molecular sieve.

[0016] Compared with the prior art, the present invention has the following beneficial effects: 1. This organoid cryopreservation cooling box can store cryopreservation tubes of different diameters and lengths, and can adapt to cryopreservation tubes of different specifications, thereby improving the utilization efficiency of the cryopreservation box. It has high versatility and can meet the needs of cryopreservation of organoid cells of various different tissues in the same organoid cryopreservation box; 2. It has the function of filtering isopropyl alcohol, which can filter the water molecules in the air that have been dissolved in the isopropyl alcohol, thereby improving the utilization rate of isopropyl alcohol and reducing the number of times the isopropyl alcohol needs to be replaced; 3. Aiming at the slightly toxic characteristics of isopropyl alcohol, the air tightness of the device is enhanced to reduce the volatilization of isopropyl alcohol.

[0017] 4. The structure is thickened to provide better explosion-proof and drop-proof capabilities. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] Figure 1 A perspective view of the present invention; Figure 2 It is an assembly diagram of the box body of the present invention; Figure 3 It is a schematic diagram of the box cover of the present invention; Figure 4 For the present invention Figure 2 A front cross-sectional view of Figure 5 For the present invention Figure 4 A partial enlarged view of the middle A; Figure 6 is a three-dimensional diagram of the box body of the present invention; Figure 7 For the present invention Figure 6 A partial enlarged view of point B in the middle; Figure 8 This is a schematic diagram of a cryopreservation tube carrier of the box body of the present invention; Fig. 9 For the present invention Figure 8 A partial enlarged view of point C in the middle; Fig.10 It is a schematic diagram of the connection between the extrusion plate and the push rod in the present invention; Fig.11 It is a schematic diagram of the connection between the vertical rod and the plug in the present invention; Fig.12 is a schematic diagram of the first spring in the present invention; Fig.13 is a schematic diagram of the central cabin in the present invention; Fig.14 Schematic diagram of the molecular sieve in the present invention.

[0019] Description of reference numerals: 1. Box body, 2. Side chamber, 3. Sliding part, 4. Arc-shaped part, 5. Cryotube carrier, 6. First sliding hole, 7. Sliding block, 8. Elastic pad, 9. Limiting column, 12. Central chamber, 13. Liquid outlet, 14. Liquid inlet, 15. Partition, 16. Inclined plate, 17. Liquid guide hole, 18. Vertical rod, 19. First spring, 20. Plug, 21. Molecular sieve, 22. Plug body, 23. Conduit, 24. Compensation chamber, 25. Extrusion plate, 26. Sleeve, 27. Second spring, 28. Extension rod, 29. Connecting rod, 30. Push rod, 31. Sealing plate, 32. Box cover. DETAILED DESCRIPTION

[0020] In order to make the purpose, technical solution and advantages of the embodiment of the present invention clearer, the technical solution of the embodiment of the present invention will be clearly and completely described below in conjunction with the accompanying drawings of the embodiment of the present invention. Obviously, the described embodiment is a part of the embodiment of the present invention, not all of the embodiments. Based on the described embodiment of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0021] Unless otherwise defined, the technical or scientific terms used herein shall have the usual meanings understood by persons of ordinary skill in the field to which the present invention belongs. "Include" or "comprises" and similar words mean that the elements or objects appearing before the word include the elements or objects listed after the word and their equivalents, without excluding other elements or objects. "Inside", "outside", "upper", "lower", "far", "near", "front", "back", etc. are only used to indicate relative positional relationships. When the absolute position of the described object changes, the relative positional relationship may also change accordingly.

[0022] The drawings in the present invention are not drawn strictly according to the actual scale, and the specific size and quantity of each structure can be determined according to actual needs. The drawings described in the present invention are only schematic diagrams of the structures.

[0023] The present invention provides an organoid cryopreservation cooling box as follows Figure 1-Figure 9 It includes a box body 1, the top surface of which is a conical surface, and side chambers 2 are provided on the conical surface along the length direction of its own multiple busbars, the bottom surface of the side chamber 2 is an inclined surface parallel to the direction of the slot, and isopropyl alcohol is provided at the bottom of the side chamber 2; the organ freezing and cooling box also includes multiple cryotube carriers 5, each of the side chambers 2 corresponds to multiple cryotube carriers 5, and each cryotube carrier 5 includes: a sliding member 3 and two elastic arc-shaped members 4; the sliding member 3 crosses the slot of the side chamber 2 and slides on the slot of the side chamber 2; the two elastic arc-shaped members 4 are both arranged in the side chamber 2, and one end of the two arc-shaped members 4 is fixedly connected to the middle part of the sliding member 3, and the other ends are staggered with each other for clamping the cryotubes. A box cover 32 is provided on the box body 1, which can reduce the contact between air and isopropyl alcohol in the side compartment 2. The box cover 32 and the box body 1 are thickened and have better explosion-proof and drop-proof capabilities. The outer diameter of the box cover 32 is 300mm and the thickness is 35mm; the outer diameter of the box body 1 is 300mm, the height is 100mm, and the cone angle of the cone is 130°-150°.

[0024] In this type of organ cryopreservation cooling box, the two arc-shaped parts 4 are staggered with each other. After the cryopreservation tubes of different diameters are inserted, the two arc-shaped parts 4 can adaptably change according to the thickness of the cryopreservation tubes, so that this type of organ cryopreservation cooling box can store cryopreservation tubes of various diameters. At the same time, the bottom of the side chamber 2 is an inclined surface, and isopropanol will gather at the lowest point of the side chamber 2. Therefore, when the cryopreservation tube is longer, the longer cryopreservation tube is preferentially inserted into the cryopreservation tube carrier 5 that is farther from the lower part of the side chamber 2. Conversely, when the cryopreservation tube is shorter, the shorter cryopreservation tube is preferentially inserted into the cryopreservation tube carrier 5 that is closer to the lower part of the side chamber 2, so that the bottoms of the longer cryopreservation tube and the shorter cryopreservation tube can both contact the liquid surface of the isopropanol, and then can be used to store cryopreservation tubes of different lengths and sizes; The organoid cryopreservation cooling box can store cryopreservation tubes of different diameters and lengths, can adapt to cryopreservation tubes of different specifications, improves the utilization efficiency of the cryopreservation box, has high versatility, and can meet the needs of cryopreserving organoid cells of various different tissues in the same organoid cryopreservation box.

[0025] In this embodiment, Figure 6-Figure 7 Both sides of each side chamber 2 are provided with a first sliding hole 6 parallel to the length direction of the slot, and the side of the first sliding hole 6 is connected to the side of the side chamber 2; the sliding member 3 is a sliding rod, which is located in the side chamber 2, and both ends of the sliding rod extend into the first sliding holes 6 on both sides of the side chamber 2, and both ends of the sliding rod are fixedly connected with a sliding block 7, and the sliding block 7 is slidably connected in the first sliding hole 6.

[0026] The sliders 7 on both sides are used to improve stability and prevent the slide bar from deviating during movement. The slide bar is also a carrier of the two arc-shaped members 4, so that the two arc-shaped members 4 are always in the notch.

[0027] In this embodiment, Figure 8-Figure 9 The inner arc surfaces of the two arc-shaped parts 4 are arranged opposite to each other, and the two arc-shaped parts 4 are staggered to form an intersection point. An elastic pad 8 is fixedly arranged on the inner arc surface of each arc-shaped part 4. A limiting column 9 for blocking the other arc-shaped part 4 is arranged on one arc-shaped part 4 in each freezing tube carrier 5, and the limiting column 9 is located on the side of the intersection point away from the sliding part 3.

[0028] The elastic pad 8 is used to provide sufficient friction for the cryotube. At the same time, when taking out the cryotube, the elastic pad 8 can effectively scrape off the isopropanol liquid on the surface of the cryotube to prevent the isopropanol from spilling and causing pollution and waste. The function of the limit column 9 is to limit the maximum embrace diameter of the two arc-shaped parts 4 to prevent the arc-shaped parts 4 from being damaged.

[0029] In this embodiment, Figure 4-Figure 5 A compensation chamber 24 is provided in the box body 1, and the compensation chamber 24 is located below the side chamber 2. The side chamber 2 and the compensation chamber 24 are separated by a layer plate, and a liquid outlet 13 is provided on the layer plate. An extrusion unit is provided at the bottom of the compensation chamber 24 for squeezing the isopropyl alcohol in the compensation chamber 24 into the side chamber 2 through the liquid outlet 13.

[0030] In this embodiment, there are multiple liquid outlets 13 on the bottom surface of the side chamber 2, and the multiple liquid outlets 13 are evenly arranged along the length direction of the bottom surface of the side chamber 2, and the number of liquid outlets 13 is the same as the number of the freezing tube carriers 5; the sliding member 3 is fixedly connected to a partition 15, and the bottom of the partition 15 is fixedly connected to an inclined plate 16, and the surface of the inclined plate 16 is slidably connected to the bottom surface of the side chamber 2, and the inclined plate 16 is provided with liquid guide holes 17. The number of liquid outlets 13 is the same as the number of liquid guide holes 17. After the sliding member 3 moves, one liquid outlet 13 can be connected to one liquid guide hole 17, so that isopropyl alcohol can enter the side chamber 2 between the multiple partitions 15 from the compensation chamber 24.

[0031] Multiple liquid outlets 13 are located on the bottom surface of the side chamber 2. A concealed pipe is provided in the bottom wall of the side chamber 2. The concealed pipes are all connected to the multiple liquid outlets 13. A main perforation is provided on the surface of the compensation chamber 24 close to the side chamber 2. The main perforation is connected to the concealed pipe, allowing the isopropyl alcohol in the compensation chamber 24 to enter the concealed pipe through the main perforation and then enter the side chamber 2 through each liquid outlet 13.

[0032] In the default state, the inclined plate 16 blocks the corresponding liquid outlet 13. Figure 5 , and multiple partitions 15 divide the side chamber 2 into multiple independent chambers. When the bottom sliding member 3 is moved, the corresponding liquid guide hole 17 on the inclined plate 16 is connected to the bottom liquid outlet 13. At this time, isopropyl alcohol enters the bottom independent chamber from the compensation chamber 24. When the amount of isopropyl alcohol in the independent chamber is appropriate, the sliding member 3 is moved again, and the inclined plate 16 is used to block the liquid outlet 13. The use method of other independent chambers is the same. The number of cryopreservation tubes used is the corresponding volume of isopropyl alcohol (cryopreservation liquid), which can make full use of the cryopreservation liquid and save the cryopreservation cost. During use, the problem of isopropyl alcohol in the independent chambers contaminating each other is avoided, ensuring that the isopropyl alcohol in the compensation chamber 24 is not contaminated.

[0033] In this embodiment, Figure 4 and Figure 11-13 A central chamber 12 is provided at the center of the cone surface, and a plurality of vertical holes are provided at the top of the central chamber 12. A valve unit is correspondingly provided in each vertical hole. The number of the vertical holes, the number of the side chambers 2 and the number of the valve units are the same. Each valve unit includes: a vertical rod 18, a first spring 19 and a plug 20; the vertical rod 18 is inserted into the vertical hole; the first spring 19 is sleeved on the vertical rod 18, and one end is connected to the vertical rod 18, and the other end is connected to the top surface of the central chamber 12, and the first spring 19 is used to realize the resetting of the vertical rod 18; the plug 20 is fixedly connected to the lower end of the vertical rod 18, and is used to block the liquid inlet 14 provided on the side wall of the side chamber 2. By moving the upper end of the vertical rod 19, the vertical rod 18 drives the plug to move, and then the liquid inlet 14 is opened.

[0034] The natural length of the first spring 19 is 30mm-40mm. Pull the vertical rod 18 upward, and the vertical rod 19 drives the plug 20 to move upward, so that the central chamber 12 replenishes isopropyl alcohol to the compensation chamber 24 through the liquid inlet 14. After the hand is released, the first spring 19 drives the vertical rod 18 and the plug 20 to reset and block the liquid inlet 14 again.

[0035] In this embodiment, Figure 4-Figure 5 and Fig.14 A molecular sieve 21 is arranged inside the central chamber 12, and the upper and lower ends of the molecular sieve 21 are fixedly connected to the box body 1. The lower end of the molecular sieve 21 is provided with a plug port connected to the inside of the molecular sieve 21, and a plug body 22 is arranged inside the plug port. The inner wall of the plug port is connected to one end of a plurality of conduits 23, and the other end of each conduit 23 is connected to a compensation chamber 24. The inner wall of the plug port is provided with an internal thread, and the plug body 22 is provided with an external thread. The plug port is threadedly matched with the plug body 22, and the end face of the plug body 22 close to the molecular sieve 21 is provided with a receiving cavity, which is screwed into the plug body 22, and the plug body 22 blocks the conduit 23 and pushes isopropanol into the molecular sieve 21. A feed port for pouring isopropanol is provided on the top of the molecular sieve 21, and the feed port has a blocking cover for blocking the feed port.

[0036] The base diameter of the molecular sieve 21 is 175 mm and the overall height is 285 mm. The molecular sieve 21 is used to filter pollutants in isopropanol and filter out other impurities mixed in during use, such as water molecules and solid particles mixed in the air. After the plug body 22 is screwed into the sealing conduit 23, the isopropanol in the accommodating cavity on the plug body 22 can be pushed into the molecular sieve 21, thereby promoting the molecular sieve 21 to quickly filter out impurities in the isopropanol.

[0037] In this embodiment, Figure 4-Figure 5 The extrusion unit includes: an extrusion plate 25, a sleeve 26 and a second spring 27; the surface of the extrusion plate 25 abuts against the lower surface of the inner part of the compensation chamber 24, and an active cavity is provided below the compensation chamber 24; the sleeve 26 is fixedly connected to the extrusion plate 25 and passes through a through hole provided at the bottom of the compensation chamber 24 to enter the active cavity; the second spring 27 is located in the sleeve 26, and its two ends respectively abut against the surface of the extrusion plate 25 and the inner wall of the active cavity, so as to push the extrusion plate 25 to move upward.

[0038] The second spring 27 always pushes the extrusion plate 25, so that the isopropyl alcohol in the compensation chamber 24 can smoothly enter the side compartment 2. When the isopropyl alcohol enters the side compartment 2, the extrusion plate 25 moves up, the elastic force of the second spring 27 is released, and the pressure in the compensation chamber 24 is reduced.

[0039] In this embodiment, Figure 5 and Fig.10The extrusion plate 25 is provided with a second sliding hole at the end near the conduit 23, and an extension rod 28 is respectively connected to the extrusion plate 25 on both sides of the second sliding hole. The two extension rods 28 are fixedly connected to the two ends of the connecting rod 29 at the bottom of the side away from the extrusion plate 25, and the connecting rod 29 is fixedly connected to one end of the push rod 30 at the side away from the extrusion plate 25, and the other end of the push rod 30 abuts the plug 20; the bottom wall of the movable cavity is provided with a third sliding hole, and the third sliding hole is slidably connected to the extension rod 28. The space between the two extension rods 28 forms a fourth sliding hole, and the fourth sliding hole is slidably connected to a sealing plate 31, which is fixedly arranged in the box body 1. The sealing plate 31 is used to ensure the sealing effect during the up and down movement of the extrusion plate 25, and the conduit 23 passes through the sealing plate 31 to enter the compensation chamber 24.

[0040] During the upward movement of the extrusion plate 25, the push rod 30 is continuously driven to push the plug 20 upward until the liquid inlet 14 is just opened, so as to replenish isopropyl alcohol in the compensation chamber. Sufficient isopropyl alcohol presses down the extrusion plate 25, so that the extrusion plate 25 and the plug 20 are reset at the same time and continue to block the liquid inlet 14.

[0041] The use method and working principle of the organoid cryopreservation cooling box of the present invention are as follows: Open the box cover 32, insert the cryotube into the cryotube carrier 5 near the edge of the cone, and drag the cryotube carrier 5 downward until the liquid guide hole 17 on the inclined plate 16 at the bottom of the cryotube carrier 5 is connected with the corresponding liquid outlet 13. At this time, isopropyl alcohol enters the lowest independent cavity from the compensation chamber 24. When the amount of isopropyl alcohol in the independent cavity is appropriate, move the sliding part 3 again, and use the inclined plate 16 to block the liquid outlet 13, so that the isopropyl alcohol liquid surface contacts the cryotube for freezing. The method of using other cryotubes is the same as the above method, and finally cover the box cover 32.

[0042] As more isopropyl alcohol enters the side compartment 2 from the compensation chamber 24, the weight of the isopropyl alcohol in the compensation chamber 24 decreases, and the second spring 27 pushes the extrusion plate 25 upward and releases the elastic force. The extrusion plate 25 moves up to a certain height, and the push rod 30 connected to the extrusion plate 25 pushes the plug 20 upward until the liquid inlet 14 is just opened, thereby replenishing isopropyl alcohol in the compensation chamber. Sufficient isopropyl alcohol presses down the extrusion plate 25, so that the extrusion plate 25 and the plug 20 are reset at the same time and continue to block the liquid inlet 14.

[0043] When it is necessary to filter the isopropanol in the compensation chamber 24, the plug 22 is properly unscrewed, the conduit 23 is opened (blocked by default), and the isopropanol in the compensation chamber 24 enters the accommodating cavity on the plug 22. After the plug 22 is screwed in again and the conduit 23 is blocked, the isopropanol in the accommodating cavity on the plug 22 can be pushed into the molecular sieve 21, promoting the molecular sieve 21 to quickly filter the impurities in the isopropanol.

[0044] The above shows and describes the basic principles, main features and advantages of the present invention. Technical personnel in this industry should understand that the present invention is not limited to the above embodiments. The above embodiments and descriptions are only preferred examples of the present invention and are not used to limit the present invention. Without departing from the spirit and scope of the present invention, the present invention may have various changes and improvements, which fall within the scope of the present invention to be protected. The scope of protection of the present invention is defined by the attached claims and their equivalents.

Claims

1. An organoid cryopreservation cooling box, comprising a box body (1), characterized in that: The top surface of the box body (1) is a conical surface, and side chambers (2) are provided on the conical surface along the length direction of the plurality of generatrixes thereof, and the bottom surface of the side chambers (2) is an inclined surface parallel to the direction of the notch, and isopropyl alcohol is present at the bottom of the side chambers (2); The organoid cryopreservation cooling box further comprises a plurality of cryopreservation tube carriers (5), each of the side chambers (2) corresponds to a plurality of cryopreservation tube carriers (5), and each cryopreservation tube carrier (5) comprises: A sliding member (3) straddles the slot of the side chamber (2) and slides on the slot of the side chamber (2); Two elastic arc-shaped members (4) are both arranged in the side chamber (2), and one end of the two arc-shaped members (4) is fixedly connected to the middle part of the sliding member (3), and the other ends are interlaced with each other, so as to clamp the cryopreservation tube.

2. The organoid cryopreservation cooling box according to claim 1, characterized in that: Both sides of each of the side chambers (2) are provided with a first sliding hole (6) parallel to the length direction of the slot, and the side surface of the first sliding hole (6) is connected to the side surface of the side chamber (2); The sliding member (3) is a sliding rod, which is located in the side chamber (2), and the two ends of the sliding rod extend into the first sliding holes (6) on both sides of the side chamber (2). Both ends of the sliding rod are fixedly connected with sliding blocks (7), and the sliding blocks (7) are slidably connected in the first sliding holes (6).

3. The organoid cryopreservation cooling box according to claim 1, characterized in that: The inner arc surfaces of the two arc-shaped members (4) are arranged opposite to each other, and the two arc-shaped members (4) are staggered to form a staggered point. An elastic pad (8) is fixedly arranged on the inner arc surface of each arc-shaped member (4). A limiting column (9) for blocking the other arc-shaped member (4) is arranged on one arc-shaped member (4) in each freezing tube carrier (5), and the limiting column (9) is located on a side of the staggered point away from the sliding member (3).

4. The organoid cryopreservation cooling box according to claim 1, characterized in that: A compensation chamber (24) is provided in the box body (1), and the compensation chamber (24) is located below the side chamber (2). The side chamber (2) and the compensation chamber (24) are separated by a layer plate, and a liquid outlet (13) is provided on the layer plate. An extrusion unit is provided at the bottom of the compensation chamber (24) for squeezing the isopropyl alcohol in the compensation chamber (24) into the side chamber (2) through the liquid outlet (13).

5. The organoid cryopreservation cooling box according to claim 4, characterized in that: There are a plurality of liquid outlets (13) on the bottom surface of the side chamber (2), and the plurality of liquid outlets (13) are evenly arranged along the length direction of the bottom surface of the side chamber (2), and the number of the liquid outlets (13) is the same as the number of the cryotube carriers (5); The sliding member (3) is fixedly connected to a partition (15), the bottom of the partition (15) is fixedly connected to an inclined plate (16), the surface of the inclined plate (16) is slidably connected to the bottom surface of the side chamber (2), and a liquid guide hole (17) is provided on the inclined plate (16). The number of the liquid outlets (13) is the same as the number of the liquid guide holes (17). After the sliding member (3) moves, one liquid outlet (13) can be connected to one liquid guide hole (17), so that isopropyl alcohol can enter the side chamber (2) between the plurality of partitions (15) from the compensation chamber (24).

6. The organoid cryopreservation cooling box according to claim 4, characterized in that: A central chamber (12) is provided at the center of the conical surface, and a plurality of vertical holes are provided at the top of the central chamber (12), wherein a valve unit is correspondingly arranged in each vertical hole, and the number of the vertical holes, the number of the side chambers (2) and the number of the valve units are all the same, and each valve unit comprises: A vertical rod (18) is inserted into the vertical hole; A first spring (19) is sleeved on the vertical rod (18), one end of the first spring being connected to the vertical rod (18) and the other end of the first spring being connected to the top surface of the central chamber (12), the first spring (19) being used to reset the vertical rod (18); The plug (20) is fixedly connected to the lower end of the vertical rod (18) and is used to block the liquid inlet (14) provided on the side wall of the side chamber (2). By moving the upper end of the vertical rod (19), the vertical rod (18) drives the plug to move, thereby opening the liquid inlet (14).

7. The organoid cryopreservation cooling box according to claim 6, characterized in that: A molecular sieve (21) is arranged inside the central chamber (12); the upper and lower ends of the molecular sieve (21) are fixedly connected to the box body (1); a plug opening is arranged at the lower end of the molecular sieve (21) and communicates with the interior of the molecular sieve (21); a plug body (22) is arranged inside the plug opening; the inner wall of the plug opening is connected to one end of a plurality of conduits (23); and the other end of each conduit (23) is connected to one of the compensation chambers (24).

8. The organoid cryopreservation cooling box according to claim 7, characterized in that: The extrusion unit comprises: An extrusion plate (25) has a surface abutting against an inner lower surface of the compensation chamber (24), and a movable cavity is provided below the compensation chamber (24); A sleeve (26) is fixedly connected to the extrusion plate (25) and passes through a through hole provided at the bottom of the compensation chamber (24) to enter the active cavity; The second spring (27) is located on the sleeve (26), with two ends respectively contacting the surface of the extrusion plate (25) and the inner wall of the movable cavity, and is used to push the extrusion plate (25) to move upward.

9. The organoid cryopreservation cooling box according to claim 8, characterized in that: The extrusion plate (25) is provided with a second sliding hole at the end thereof close to the conduit (23); an extension rod (28) is respectively connected to the extrusion plate (25) on both sides of the second sliding hole; the two extension rods (28) are respectively fixedly connected to two ends of a connecting rod (29) at the bottom of the side away from the extrusion plate (25); the connecting rod (29) is fixedly connected to one end of a push rod (30) at the side away from the extrusion plate (25); and the other end of the push rod (30) abuts against the plug (20); The bottom wall of the movable cavity is provided with a third sliding hole, the third sliding hole is slidably connected to the extension rod (28), the space between the two extension rods (28) forms a fourth sliding hole, the fourth sliding hole is slidably connected to a sealing plate (31), the sealing plate (31) is fixedly arranged in the box body (1), the sealing plate (31) is used to ensure the sealing effect during the upward and downward movement of the extrusion plate (25), and the conduit (23) passes through the sealing plate (31) and enters the compensation chamber (24).

10. The organoid cryopreservation cooling box according to claim 7, characterized in that: The inner wall of the plug opening is provided with an internal thread, the plug body (22) is provided with an external thread, the plug opening and the plug body (22) are threadedly matched, and the end surface of the plug body (22) close to the molecular sieve (21) is provided with a receiving cavity, and the plug body (22) is screwed into the plug body, so that the plug body (22) blocks the conduit (23) and pushes the isopropanol into the molecular sieve (21).

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