Laboratory mouse fixer adaptive to X-ray irradiator and use method

Abstract

The invention belongs to the technical field of laboratory mouse fixing, and particularly relates to a laboratory mouse fixing device matched with an X-ray irradiator and a using method, and the laboratory mouse fixing device specifically comprises a ray shielding cover used for shielding the X-ray irradiator and a limiting fixing disc used for fixing a laboratory mouse. A fixing device cover plate used for fixing an experimental mouse is arranged at the top of the limiting fixing disc in a buckled mode, and limb fixing discs are fixedly installed at the positions, corresponding to the four limbs of the experimental mouse, of the two sides of the limiting fixing disc correspondingly, in the scheme, through the fixing structures correspondingly arranged at the four limbs of the experimental mouse, the activity space of the experimental mouse can be effectively limited, and the experimental mouse can be prevented from being damaged. According to the fixing device, the stability of an irradiation experiment is guaranteed, the ray shielding cover and the fixing device are correspondingly buckled and fixed, disassembly is convenient, meanwhile, shielding protection of a non-irradiation part is effectively guaranteed, and therefore the problems that an existing fixing device is poor in irradiation stability, and a sampling organ is inconvenient to display are solved.

Description

Technical Field

[0001] The invention belongs to the technical field of laboratory mouse fixation, and particularly relates to a laboratory mouse fixer adapted for an X-ray irradiator and a use method thereof. Background Art

[0002] Esophageal cancer is a common upper gastrointestinal malignancy and is currently ranked as the eighth most common cancer in the world. The high incidence and mortality of this disease make it a major public health issue in my country. Radiotherapy is one of the core means of treating esophageal cancer and is involved in almost every stage of disease management. For patients with locally advanced esophageal cancer, neoadjuvant chemoradiotherapy combined with surgery is an internationally recognized standard treatment strategy that can effectively reduce tumor size, increase R0 resection rate and prolong the patient's disease-free survival. For patients with a higher risk of postoperative recurrence, adjuvant radiotherapy can reduce the local recurrence rate.

[0003] In the specific experimental process, experimental mice were selected to carry out radiation pulmonary fibrosis (RPF) pathological reactions to assist in the development of therapeutic vaccines based on relevant antigens. In order to adapt to the RS2000pro biological X-ray irradiator, an experimental living body fixing structure needs to be designed to ensure the stability of the experimental mice during radiotherapy and surgical sampling. The existing laboratory mouse fixing device, referring to the laboratory mouse fixing device disclosed in the Chinese utility model patent with application number CN202123170403.5, is provided with a working area that can be adsorbed by a magnet on the base plate, and then provided with multiple fixing seats. When in use, the clamping heads of the multiple fixing seats are respectively clamped on the limbs of the laboratory mouse. This structure has a wide range of adaptability and is a mostly selected fixing structure. However, within the irradiation range of the X-ray irradiator, the position of the laboratory mouse needs to be limited and fixed. When this type of equipment is used, the buffer space reserved for the laboratory mouse is large, and it is not suitable for related experimental fixation; Another example is a mouse fixator disclosed in the Chinese invention patent application number CN201410217986.8. This type of fixator mainly uses a limiting structure to perform local positioning. When this structure is used, it cannot be used in organ irradiation experiments and subsequent lung lobe sampling operations because the limb structures and chest organs of experimental mice are gathered together. In irradiation experiments, it is necessary to ensure that the experimental mice are fixed in the irradiation area of ​​the X-ray irradiator, to ensure that the experimental mice are shielded from radiation outside the irradiation range, and to ensure the stability of the shielding mask. When adapting to the sampling process after radiotherapy, the organs should also be dispersed to facilitate sampling. For this reason, we propose an experimental mouse fixer adapted to the X-ray irradiator and a method of use. Summary of the invention

[0004] The purpose of the present invention is to provide a laboratory mouse fixer adapted for an X-ray irradiator and a method of use, so as to solve the problems raised in the above-mentioned background technology.

[0005] In order to achieve the above-mentioned purpose, the present invention adopts the following technical scheme: an experimental mouse holder adapted for an X-ray irradiator, comprising a ray shielding cover for shielding the X-ray irradiator, and a limit fixing plate for fixing the experimental mouse, a holder cover for fixing the experimental mouse is buckled at the top position of the limit fixing plate, wherein limb fixing plates are fixedly installed at the positions of the limbs of the experimental mouse on both sides of the limit fixing plate, and a limb restraint tube 1 for fixing the wrist of the experimental mouse is arranged at the end position of the limb fixing plate, wherein the limb fixing plates on the holder cover are fixedly installed at the positions of the limbs of the experimental mouse A limb fixing cover and a limb restraint tube 2 are installed at the positions of the plate and the limb restraint tube 1, and a neck pressing frame is fixedly installed on the fixer cover plate, wherein the wrist of the experimental mouse passes through the limiting space formed by the buckling of the limb restraint tube 1 and the limb restraint tube 2, and is fixed by adding a strap, the front end of the limiting fixing plate and the fixer cover plate is detachably connected to the head fixer, and an oxygen supply tube for anesthesia is connected and fixed to the head fixer, and a tail placement slot for the experimental mouse is opened at the rear end position of the limiting fixing plate and the fixer cover plate, and a lifting slot is opened through the middle position of the bottom of the limiting fixing plate; The radiation shielding cover is buckled at the top position of the fixing cover plate, wherein the radiation shielding cover is provided with a cover plate structure for placing the X-ray shielding.

[0006] Furthermore, the cover plate structure arranged on the radiation shielding cover includes a short plate shielding cover and a long plate shielding cover, and a slide groove for placing the short plate shielding cover and the long plate shielding cover is vertically opened on the radiation shielding cover, and a positioning hole is opened on the limb fixing cover, wherein a fixing column is fixedly installed at the position corresponding to the positioning hole on the radiation shielding cover, and the positioning hole and the fixing column are fixed to each other.

[0007] Furthermore, a head holder for fixing the head of the experimental mouse is provided inside the head holder, wherein a supporting pad protrusion for placing the head holder is fixedly installed at the bottom position of the head holder, and a hole for movably passing the oxygen supply tube is opened on the head holder, wherein the end position of the oxygen supply tube passes through the head holder, the rubber buffer and the head holder in sequence, and is fixedly connected to the oxygen supply mimic hood.

[0008] Furthermore, a limit cover is fixedly installed on the head fixer, and a limit slide is fixedly installed at a corresponding position on the head retaining frame, wherein the limit slide slides along the inner wall of the limit cover.

[0009] Furthermore, a buckle is fixedly installed on the head fixer at a connection position corresponding to the fixer cover plate, and a protrusion is fixedly installed on the head fixer at a connection position corresponding to the limit fixing plate.

[0010] Furthermore, a spreader for supporting the incised wound of the experimental mouse is fixedly installed on the limiting fixing plate, and a positioning plate for fixing the spreader is symmetrically installed at a position on one side of the limiting fixing plate corresponding to the limb fixing plate, wherein the bottom position of the spreader is screwed together with the positioning plate through a rotating shaft structure, the inside of the spreader is screwed together with an adjusting screw, the outside of the adjusting screw is screwed together with a locking nut, the adjusting screw is rotatably installed on the limiting bracket, and a support hook for supporting the incised wound is fixedly installed on the limiting bracket.

[0011] Furthermore, the rotating shaft structure includes a lifting screw that is screwed on the positioning plate and a pillow block with a hole that is fixed at the bottom of the spreader, wherein the inner ring of the bearing of the pillow block with a hole is provided with a fixed thread groove for use with the lifting screw, and the outer side of the lifting screw is screwed on a limiting nut.

[0012] Furthermore, a support block 1 is provided on the limiting fixing plate at the position corresponding to the shoulder blade of the experimental mouse, wherein support blocks 2 are fixedly installed on the limiting fixing plate at the positions on both sides of the abdominal cavity of the experimental mouse, and a card block structure is fixedly installed on the bottom of the limb fixing cover, wherein a card slot for fixing the card block structure is provided on the limb fixing plate.

[0013] In a method for using an experimental mouse fixer adapted for an X-ray irradiator, the steps of fixing the experimental mouse for irradiation experiment by the X-ray irradiator include: S1: Preparation stage, 1. Before use, check whether the laboratory mouse holder can be used normally, and use 75% alcohol to wipe and disinfect the radiation shielding cover, limit fixing plate, holder cover, head holder, strap, spreader and positioning plate, as well as the above parts that come into contact with the laboratory mouse; Second, select 8-10 week old, C57BL / 6 male experimental mice, n=16 per group; 3. Anesthesia operation: drip anesthetic onto medical cotton balls, place the cotton balls in a sealed container, then place the experimental mouse in the container to allow it to inhale the anesthetic until the experimental mouse enters an anesthetized state; S2: Fix the experimental mouse. Place the experimental mouse prone on the experimental table, and place the limit fixing plate on the back of the experimental mouse. Pull up the back skin of the experimental mouse from the position of the pulling groove, and then turn the limit fixing plate 180 degrees to place the experimental mouse on the limit fixing plate in a supine position. Place the head of the experimental mouse on the head holder, and place the mouth and nose of the experimental mouse at the position of the oxygen supply mimic hood. Fill the inner side of the oxygen supply tube with anesthetic cotton balls, and use the oxygen supply mimic hood to continuously anesthetize the experimental mouse with oxygen. Then place the limbs of the anesthetized experimental mouse at the corresponding positions of the limb fixing plate, and place the wrist of the experimental mouse close to the end of the limb restraint tube. At the position of the head, the tail of the experimental mouse is placed outside from the position of the tail placement slot on the limiting fixing plate, and the fixer cover is buckled on the top of the limiting fixing plate, so that the fixer cover is fixed to the head fixer and the limiting fixing plate, wherein the limb fixation cover and the limb restraint tube 2 are buckled with the limb fixation plate and the limb restraint tube 1 respectively, and the limbs of the experimental mouse are pulled up so that the wrist of the experimental mouse completely passes through the limiting space of the limb restraint tube 1 and the limb restraint tube 2, and the straps are respectively set on the limb restraint tube 1 and the limb restraint tube 2 corresponding to the position of the wrist bone of the experimental mouse to complete the fixation of the limbs of the experimental mouse, and then the tail of the experimental mouse is bent and fitted on the outside of the limiting fixing plate; S3: Experimental operation: buckle the fixing column on the radiation shielding cover to the position of the positioning hole on the fixing cover plate to complete the installation of the radiation shielding cover, then place the experimental mouse according to the irradiation position of the X-ray irradiator standard sample room, and choose to add the long plate shielding cover or the short plate shielding cover to the slide position of the radiation shielding cover. After the irradiation experimental mouse is fixed, close the X-ray irradiator standard sample room door to carry out the irradiation experiment; S4: Release the fixation. After the irradiation experiment is completed, open the door of the sample chamber of the X-ray irradiator, place the experimental mouse on the experimental table, and then move the fixing column on the radiation shielding cover and the positioning hole on the fixer cover upward and separate them. Remove the strap from the fixed position of the limb restraint tube and the neck pressure frame, and throw it into the waste collection bin. Move the limit fixing plate and the fixer cover upward and separate them. Then separate the head fixer from the limit fixing plate, and remove the irradiated experimental mouse. S5: Cleaning and disinfection: disassemble the above-mentioned experimental mouse holder, rinse the parts with clean water to remove residual stains and hair, then soak them in disinfectant water for 15 minutes, take them out and dry them for storage.

[0014] Furthermore, when collecting the lung lobe tissue of the above-mentioned experimental mice, the above-mentioned fasted experimental mice are placed in a sterile surgical environment, and the above-mentioned steps S1 and S2 are repeated to fix the irradiated experimental mice; Fix the spreader on the position of the positioning plate and adjust it for use. When the incision of the experimental mouse is exposed, fix the support hooks on both sides of the incision to support the incision. After sampling, remove the support hooks from the incision position, disinfect and suture the incision of the experimental mouse, remove the spreader from the position of the positioning plate, repeat the steps of removing the experimental mouse from the fixator in S4, and then repeat the steps of S5 to disinfect the above-mentioned experimental mouse fixator to complete the disinfection and storage.

[0015] Compared with the prior art, the present invention has the following beneficial effects: 1. In this scheme, by correspondingly setting the fixed structure at the position of the limbs of the experimental mouse, the activity space of the experimental mouse can be effectively limited to ensure the stability of the irradiation experiment. By correspondingly buckling and fixing the radiation shielding cover with the fixture, it is convenient to disassemble and effectively ensure the shielding protection of the non-irradiated parts.

[0016] 2. The correspondingly arranged buffer structure for the head of the experimental mouse can prevent the skin from being damaged due to the struggle of the experimental mouse after the anesthesia fails, and the hanging spreader 6 can effectively realize the auxiliary spread of the incision, thereby increasing the stability of the fixator. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] The accompanying drawings are used to provide further understanding of the present invention and constitute a part of the specification. They are used to explain the present invention together with the embodiments of the present invention and do not constitute a limitation of the present invention.

[0018] Figure 1 It is a schematic diagram of the overall structure of the present invention; Figure 2 For the present invention Figure 1 Schematic diagram of the split structure; Figure 3 It is a schematic diagram of the combined structure of the radiation shielding cover and the long plate shielding cover of the present invention; Figure 4 It is a schematic diagram of the reverse structure of the combination of the limit fixing plate and the fixing cover plate in the present invention; Figure 5 It is a schematic diagram of the disassembled structure of the head fixer of the present invention; Figure 6 This is a schematic diagram of the structure of the experimental mouse fixer in the present invention with a spreader added; Figure 7 It is a schematic diagram of the split structure of the spreader of the present invention; In the figure: 1, ray shielding cover; 11, short board shielding cover; 12, long board shielding cover; 13, fixing column; 2. Limit fixing plate; 21. Limb fixing plate; 22. Limb restraining tube 1; 23. Supporting block 1; 24. Supporting block 2; 25. Tail placement slot; 26. Lifting slot; 3. Fixer cover; 31. Limb fixing cover; 32. Limb restraining tube II; 33. Neck pressing frame; 34. Positioning hole; 4. Head fixer; 41. Head holder; 42. Oxygen supply mimic cover; 43. Oxygen supply tube; 44. Rubber buffer; 45. Limit cover; 46. Limit slide plate; 47. Support pad protrusion; 5. Drawstring; 6. Spreader; 61. Adjusting screw; 62. Limiting bracket; 63. Support hook; 64. Pillow block with hole; 65. Limiting nut; 66. Lifting screw; 7. Positioning plate. DETAILED DESCRIPTION

[0019] The technical solutions in the embodiments of the present invention will be described clearly and completely below in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, rather than all the embodiments.

[0020] In the description of the present invention, it is necessary to understand that the terms "upper", "lower", "front", "back", "left", "right", "top", "bottom", "inside", "outside", etc., indicating the orientation or position relationship are based on the orientation or position relationship shown in the drawings, and are only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as a limitation on the present invention.

[0021] Reference Figure 1 - Figure 7 The present invention proposes a technical solution: a laboratory mouse holder adapted for an X-ray irradiator, comprising a ray shielding cover 1 for shielding the X-ray irradiator, and a limiting fixing plate 2 for fixing the laboratory mouse, a holder cover plate 3 for fixing the laboratory mouse is buckled at the top position of the limiting fixing plate 2, wherein limb fixing plates 21 are fixedly installed at both sides of the limiting fixing plate 2 corresponding to the positions of the limbs of the laboratory mouse, and a limb restraint tube 1 22 for fixing the wrist of the laboratory mouse is arranged at the end position of the limb fixing plate 21, wherein a limb fixing cover 31 and a limb restraint tube 2 32 are correspondingly installed at the positions of the limb fixing plate 21 and the limb restraint tube 1 22 on the holder cover plate 3, and a neck pressing frame 33 is fixedly installed on the holder cover plate 3, wherein after the wrist of the laboratory mouse passes through the limiting space formed by buckling the limb restraint tube 1 22 and the limb restraint tube 2 32, Specifically, a card block structure is fixedly installed at the bottom of the limb fixing cover 31, wherein a card slot for fixing the card block structure is correspondingly opened on the limb fixing plate 21, and the two are tightly buckled with each other to fix the limit fixing plate 2 and the fixer cover plate 3; The front ends of the position-limiting fixing plate 2 and the fixing cover plate 3 are detachably connected to the head fixing plate 4, and an oxygen supply tube 43 for anesthesia is connected and fixed to the head fixing plate 4. The rear ends of the position-limiting fixing plate 2 and the fixing cover plate 3 are provided with a groove 25 for placing the tail of the experimental mouse, and the middle position of the bottom of the position-limiting fixing plate 2 is provided with a lifting groove 26; The radiation shielding cover 1 is buckled at the top position of the fixing cover plate 3, wherein the radiation shielding cover 1 is provided with a cover plate structure for placing X-ray shielding.

[0022] The cover plate structure arranged on the radiation shielding cover 1 includes a short shielding cover 11 and a long shielding cover 12, and a slide groove for placing the short shielding cover 11 and the long shielding cover 12 is vertically opened on the radiation shielding cover 1, and a positioning hole 34 is opened on the limb fixing cover 31, wherein a fixing column 13 is fixedly installed at the position corresponding to the positioning hole 34 on the radiation shielding cover 1, and the positioning hole 34 and the fixing column 13 are fixed to each other.

[0023] A head holder 41 for fixing the head of the experimental mouse is arranged inside the head holder 4, wherein a supporting protrusion 47 for placing the head holder 41 is fixedly installed at the bottom position of the head holder 4, and a hole for movably passing the oxygen supply tube 43 is opened on the head holder 4, wherein the end position of the oxygen supply tube 43 passes through the head holder 4, the rubber buffer 44 and the head holder 41 in sequence, and is fixedly connected to the oxygen supply mimetic cover 42.

[0024] It should be noted that in order to adapt to the RS2000pro biological X-ray irradiator, the rubber buffer 44 should be made of medical rubber material to avoid the problem of metal affecting the blurred imaging of the irradiated rays; A limiting cover 45 is fixedly installed on the head fixer 4, and a limiting slide 46 is fixedly installed at a corresponding position on the head retaining frame 41, wherein the limiting slide 46 slides along the inner wall of the limiting cover 45, and this part mainly plays the role of limiting buffering. Since the skin at the mouth and nose of the experimental mouse is relatively fragile, it should be properly protected to avoid trauma.

[0025] Among them reference Figure 5 In the figure, a buckle is fixedly installed on the head fixer 4 at the connection position corresponding to the fixer cover 3, and a slot structure is opened on the fixer cover 3, and the two are adapted and fixed to each other, wherein the slot structure on the fixer cover 3 has a buffer groove that can be manually removed, and a protrusion is fixedly installed on the head fixer 4 at the connection position corresponding to the limit fixing disk 2, and a groove is opened at the corresponding position on the limit fixing disk 2 for docking with the protrusion structure on the head fixer, thereby realizing the combined fixation of the limit fixing disk 2, the fixer cover 3 and the head fixer 4.

[0026] The limiting fixing plate 2 is fixedly installed with a spreader 6 for supporting the incised wound of the experimental mouse, and a positioning plate 7 for fixing the spreader 6 is symmetrically installed at a position on one side of the limiting fixing plate 2 corresponding to the limb fixing plate 21, wherein the bottom position of the spreader 6 is screwed together with the positioning plate 7 through a rotating shaft structure, the inside of the spreader 6 is screwed together with an adjusting screw 61, the outer side of the adjusting screw 61 is screwed together with a locking nut, the adjusting screw 61 is rotatably installed on the limiting bracket 62, and the limiting bracket 62 is fixedly installed with a support hook 63 for supporting the incised wound.

[0027] The rotating shaft structure includes a lifting screw 66 screwed on the positioning plate 7 and a perforated pillow block 64 fixed at the bottom of the spreader 6, wherein the inner ring of the bearing of the perforated pillow block 64 is provided with a fixed thread groove used in conjunction with the lifting screw 66, and the outer side of the lifting screw 66 is screwed and screwed with a limiting nut 65.

[0028] It is worth noting that the structure of the perforated shaft block 64 is an inner and outer ring bearing structure, and the outer ring portion of the perforated shaft block 64 is fixed to the bottom of the fixed spreader 6 housing by interference fit, the inner ring thread groove of the perforated shaft block 64 cooperates with the lifting screw 66 to be screwed together and fixed, and there is no metal structure on the positioning plate 7.

[0029] In order to facilitate irradiation experiments and sampling operations, a support block 23 is provided on the limiting fixing plate 2 of the experimental mouse fixator at the position corresponding to the shoulder blade of the experimental mouse, and a support block 24 is fixedly installed on the limiting fixing plate 2 at the positions corresponding to the two sides of the abdominal cavity of the experimental mouse.

[0030] Embodiment 1

[0031] refer to Figure 2 and Figure 3 In the X-ray irradiator irradiation experiment, the relevant steps are: Before use, check whether the experimental mouse holder can be used normally, and use 75% alcohol to wipe and disinfect the radiation shielding cover 1, the limit fixing plate 2, the holder cover 3, the head holder 4, the strap 5, the spreader 6 and the positioning plate 7, as well as the above parts that come into contact with the experimental mouse; 8-10-week-old C57BL / 6 male experimental mice were selected, n = 16 per group; Anesthesia operation: drip anesthetic onto medical cotton balls, place the cotton balls in a sealed container, and then place the experimental mouse in the container to allow it to inhale the anesthetic until the experimental mouse enters an anesthetized state; Instrument preparation: Turn on the RS2000pro biological X-ray irradiator in advance, preheat and stabilize it, and set the irradiation to 20 Gy per shot according to the experimental requirements; Place the experimental mouse prone on the experimental table, and buckle the limiting fixing plate 2 on the back of the experimental mouse. Pull up the back skin of the experimental mouse from the position of the pulling groove 26, and then turn the limiting fixing plate 2 180 degrees to place the experimental mouse on the limiting fixing plate 2 in a supine position. Place the head of the experimental mouse on the head holder 41, and the mouth and nose of the experimental mouse on the position of the oxygen supply mimetic hood 42. Fill the inner side of the oxygen supply tube 43 with anesthetic cotton balls, and use the oxygen supply mimetic hood 42 to continuously anesthetize the experimental mouse with oxygen. Then place the limbs of the anesthetized experimental mouse on the corresponding positions of the limb fixing plate 21, and place the wrist of the experimental mouse on the end position of the limb restraint tube 22. The tail is placed outside the position of the tail placement groove 25 on the limiting fixing plate 2, and the fixer cover plate 3 is buckled on the top of the limiting fixing plate 2, so that the fixer cover plate 3 is fixed to the head fixer 4 and the limiting fixing plate 2, wherein the limb fixing cover 31 and the limb restraint tube 2 32 are buckled with the limb fixing plate 21 and the limb restraint tube 1 22 respectively, and the limbs of the experimental mouse are pulled up so that the wrist of the experimental mouse completely passes through the limiting space of the limb restraint tube 1 22 and the limb restraint tube 2 32, and the strap 5 is respectively set on the limb restraint tube 1 22 and the limb restraint tube 2 32 corresponding to the position of the wrist bone of the experimental mouse, and the limbs of the experimental mouse are fixed, and then the tail of the experimental mouse is bent and fitted on the outside of the limiting fixing plate 2; The fixing column 13 on the radiation shielding cover 1 is buckled into the position of the positioning hole 34 on the fixing cover plate 3 to complete the installation of the radiation shielding cover 1, and then the experimental mouse is placed according to the irradiation position of the X-ray irradiator standard sample chamber, and the long plate shielding cover 12 or the short plate shielding cover 11 is selected to be added to the slide groove position of the radiation shielding cover 1, and the irradiation experimental mouse is fixed. In this embodiment, chest irradiation is used as an example, and the two short plate shielding covers 11 cover the head and lower abdomen of the experimental mouse respectively, exposing only the chest to prevent other organs from being exposed to irradiation; after the experiment is completed, the door of the X-ray irradiator standard sample chamber is closed to carry out the irradiation experiment; After the irradiation experiment is completed, open the door of the sample chamber of the X-ray irradiator, move the experimental mouse covered with the radiation shielding cover 1 to the experimental table, then move the fixing column 13 on the radiation shielding cover 1 and the positioning hole 34 on the fixer cover 3 upward and separate them, remove the strap 5 from the fixed position of the limb restraint tube 22 and the neck compression frame 33, and throw them into the waste collection bin, move the limit fixing plate 2 and the fixer cover 3 upward and separate them, then separate the head fixer 4 from the limit fixing plate 2, and remove the irradiated experimental mouse.

[0032] Disassemble the above-mentioned experimental mouse holder, rinse the parts with clean water to remove residual stains and hair, then soak them in disinfectant water for 15 minutes, take them out and dry them for storage.

[0033] Embodiment 2

[0034] When collecting lung lobe tissues from the above-mentioned experimental mice, the above-mentioned experimental mice and normal mice were raised independently and selected as the RPF group and the control group. The cycle was 12-16 hours, during which they were fasted but not watered. Afterwards, the experimental mice and normal mice were placed in a sterile surgical environment respectively; Check whether the experimental mouse holder can be used normally, and use 75% alcohol to wipe and disinfect the radiation shielding cover 1, the limit fixing plate 2, the holder cover plate 3, the head holder 4, the strap 5, the spreader 6 and the positioning plate 7, as well as the above-mentioned parts that come into contact with the experimental mouse; Prepare surgical instruments including scalpels, surgical scissors, tweezers, 75% alcohol, saline, paraformaldehyde, etc. When anesthetizing experimental mice and normal mice, sodium pentobarbital or isoflurane is often used for anesthesia. When intraperitoneal injection of sodium pentobarbital is used, the dose is generally 30-50 mg / kg body weight. When using isoflurane anesthesia, the induction concentration is generally 3% to 5%, and the maintenance concentration is 1.5% to 2.5%.

[0035] After anesthesia takes effect, the mouse is fixed on the operating table in a supine position, and the chest is disinfected with 75% alcohol, and extensive disinfection is performed from the neck to the abdomen. The tissue in the chest cavity is carefully separated, the lungs are exposed, and the morphology and position of the lung lobes are observed.

[0036] The specific steps of fixing the irradiated experimental mice and normal mice can refer to the relevant steps in Example 1; Then install and fix the spreader 6 at the position of the positioning plate 7 and adjust it for standby use. When the incision of the experimental mouse is exposed, buckle and fix the support hook 63 on both sides of the incision of the experimental mouse to support the incision. This part mainly supports the surface skin. The skin should be cut along the midline of the abdomen of the surgical mouse, and the sternum should be cut upward from the xiphoid process to expose the chest cavity; One lobe of the left lung was removed from the operated mice for histopathological analysis, and 100 mg of the four lobes of the right lung were taken for immunopeptide group analysis. The removed lung lobe samples were immediately placed in a culture dish filled with physiological saline, and the samples were placed in paraformaldehyde fixative for storage. In addition, due to the need for cardiac perfusion, the mouse lung tissue for single-cell sequencing needed to be prepared separately.

[0037] After sampling is completed, the support hook 63 is removed from the incision position, and the incision of the surgical mouse is disinfected and sutured. The spreader 6 is removed from the position of the positioning plate 7, and the steps of removing the experimental mouse from the fixator are repeated. The disinfection steps are repeated to disinfect the above surgical mouse fixator to complete the disinfection and storage.

[0038] The specific steps for using the spreader 6 are as follows: screw the top of the lifting screw 66 clockwise from bottom to top to penetrate the screw hole on the positioning plate 7, and screw the limiting nut 65 counterclockwise to the outside of the lifting screw 66, press the inner circle of the hole-bearing pillow block 64, screw the top of the lifting screw 66 and the inner circle of the hole-bearing pillow block 64 clockwise to fix it, release the pressing part, and rotate the lifting screw 66 clockwise to adjust the use height of the spreader 6 to the top; screw the adjusting screw 61 clockwise along the screw hole on the spreader 6 to adjust the outward length of the support hook 63 to above the lung lobe of the experimental mouse, and rotate the locking nut on the adjusting screw 61 clockwise until the above-mentioned locking nut is tightly fitted with the spreader 6; rotate the lifting screw 66 counterclockwise until the support hook 63 moves to the incision position, and rotate the limiting nut 65 counterclockwise along the thread on the lifting screw 66 Screw it together and move it down until it is close to the top of the positioning plate 7, and then buckle the skin incision of the experimental mouse on the support hooks 63 on both sides respectively. When the lung lobe sample is taken and placed, the skin incision of the experimental mouse is separated from the support hooks 63. After the wound of the experimental mouse is sutured, the limiting nut 65 is rotated clockwise along the thread on the lifting screw 66, wherein the rotation height of the limiting nut 65 is greater than the fixed height of the lifting screw 66 and the inner circle of the hole-bearing pillow block 64. At this time, press the inner circle of the hole-bearing pillow block 64 and rotate the lifting screw 66 counterclockwise to separate the lifting screw 66 from the inner circle thread of the hole-bearing pillow block 64, and complete the disassembly of the spreader 6, and then continue to rotate the limiting nut 65 clockwise along the lifting screw 66 until the two are completely separated, and then rotate the lifting screw 66 counterclockwise from the positioning plate 7 to remove it. At this time, repeat the above disassembly steps to remove the experimental mouse.

[0039] The above description is only a preferred specific implementation manner of the present invention, but the protection scope of the present invention is not limited thereto. Any technician familiar with the technical field can make equivalent replacements or changes according to the technical scheme and inventive concept of the present invention within the technical scope disclosed by the present invention, which should be covered by the protection scope of the present invention.

Claims

1. A laboratory mouse holder adapted for an X-ray irradiator, comprising a ray shielding cover (1) for shielding the X-ray irradiator, and a limiting fixing plate (2) for fixing the laboratory mouse, characterized in that: A fixer cover plate (3) for fixing the experimental mouse is buckled at the top position of the limit fixing plate (2), wherein limb fixing plates (21) are fixedly installed at the positions corresponding to the limbs of the experimental mouse on both sides of the limit fixing plate (2), and a limb restraint tube 1 (22) for fixing the wrist of the experimental mouse is arranged at the end position of the limb fixing plate (21), wherein a limb fixing cover (31) and a limb restraint tube 2 (32) are correspondingly installed at the positions corresponding to the limb fixing plate (21) and the limb restraint tube 1 (22) on the fixer cover plate (3), and a limb restraint tube 2 (32) is fixedly installed on the fixer cover plate (3). A neck pressing frame (33), wherein the wrist of the experimental mouse passes through the limiting space formed by the limb restraint tube 1 (22) and the limb restraint tube 2 (32), and is fixed by adding a strap (5); the front end of the limiting fixing plate (2) and the fixer cover plate (3) are detachably connected to the head fixer (4); an oxygen supply tube (43) for anesthesia is connected and fixed to the head fixer (4); a tail placement groove (25) for the experimental mouse is provided at the rear end of the limiting fixing plate (2) and the fixer cover plate (3); and a lifting groove (26) is provided through the middle of the bottom of the limiting fixing plate (2); The radiation shielding cover (1) is buckled at the top position of the fixing cover plate (3), wherein the radiation shielding cover (1) is provided with a cover plate structure for placing X-ray shielding.

2. The experimental mouse holder adapted for an X-ray irradiator according to claim 1, characterized in that: The cover plate structure provided on the radiation shielding cover (1) comprises a short shielding cover (11) and a long shielding cover (12), and a slide groove for placing the short shielding cover (11) and the long shielding cover (12) is vertically provided on the radiation shielding cover (1), and a positioning hole (34) is provided on the limb fixing cover (31), wherein a fixing column (13) is fixedly installed at a position corresponding to the positioning hole (34) on the radiation shielding cover (1), and the positioning hole (34) and the fixing column (13) are fixedly matched with each other.

3. The experimental mouse holder adapted for an X-ray irradiator according to claim 1, characterized in that: The head holder (4) is provided inside the head holder (4) for fixing the head of the experimental mouse, wherein a support protrusion (47) for placing the head holder (41) is fixedly installed at the bottom position of the head holder (4), and a hole for movably passing an oxygen supply tube (43) is opened on the head holder (4), wherein the end position of the oxygen supply tube (43) passes through the head holder (4), the rubber buffer (44) and the head holder (41) in sequence, and is fixedly connected to the oxygen supply mimic cover (42).

4. The experimental mouse holder adapted for an X-ray irradiator according to claim 3, characterized in that: A limit cover (45) is fixedly mounted on the head holder (4), and a limit slide plate (46) is fixedly mounted at a corresponding position on the head holder (41), wherein the limit slide plate (46) slides in a limit manner along the inner wall of the limit cover (45).

5. The experimental mouse holder adapted for an X-ray irradiator according to claim 4, characterized in that: A buckle is fixedly mounted on the head fixer (4) at a connection position corresponding to the fixer cover plate (3), and a protrusion is fixedly mounted on the head fixer (4) at a connection position corresponding to the position limiting fixing plate (2).

6. The experimental mouse holder adapted for an X-ray irradiator according to claim 1, characterized in that: The limit fixing plate (2) is fixedly mounted with a spreader (6) for supporting the incised wound of the experimental mouse, and a positioning plate (7) for fixing the spreader (6) is symmetrically mounted at a position on one side of the limit fixing plate (21) corresponding to the limb fixing plate (21), wherein the bottom position of the spreader (6) is screwed and connected to the positioning plate (7) via a rotating shaft structure, an adjusting screw (61) is screwed inside the spreader (6), a locking nut is screwed outside the adjusting screw (61), the adjusting screw (61) is rotatably mounted on a limit bracket (62), and a supporting hook (63) for supporting the incised wound is fixedly mounted on the limit bracket (62).

7. The experimental mouse holder adapted for an X-ray irradiator according to claim 6, characterized in that: The rotating shaft structure comprises a lifting screw (66) screwed on the positioning plate (7) and a pillow block (64) fixed at the bottom of the spreader (6), wherein the inner ring of the bearing of the pillow block (64) is provided with a fixed thread groove for use with the lifting screw (66), and the outer side of the lifting screw (66) is screwed on the limiting nut (65).

8. The experimental mouse holder adapted for an X-ray irradiator according to claim 1, characterized in that: A support block 1 (23) is provided on the position-limiting fixing plate (2) at a position corresponding to the shoulder blade of the experimental mouse, wherein support blocks 2 (24) are fixedly installed on the position-limiting fixing plate (2) at positions corresponding to both sides of the abdominal cavity of the experimental mouse, and a clamping block structure is fixedly installed on the bottom of the limb fixing cover (31), wherein a clamping slot for fixing the clamping block structure is correspondingly provided on the limb fixing plate (21).

9. A method for using the laboratory mouse holder adapted for an X-ray irradiator according to any one of claims 1 to 8, characterized in that: The steps for fixing experimental mice in the X-ray irradiator irradiation experiment include: S1: Preparation stage, 1. Before use, check whether the laboratory mouse holder can be used normally, and use 75% alcohol to wipe and disinfect the radiation shielding cover (1), the limit fixing plate (2), the holder cover (3), the head holder (4), the strap (5), the spreader (6) and the positioning plate (7), as well as the above-mentioned parts that come into contact with the laboratory mouse; Second, select 8-10 week old C57BL / 6 male experimental mice, n=16 per group; 3. Anesthesia operation: drip anesthetic onto medical cotton balls, place the cotton balls in a sealed container, and then place the experimental mice in the container to allow them to inhale the anesthetic until they enter an anesthetized state; S2: Fixing the experimental mouse: Place the experimental mouse in a prone position on the experimental table, and buckle the limiting fixing plate (2) on the back of the experimental mouse. Pull up the back skin of the experimental mouse from the position of the pulling groove (26), and then turn the limiting fixing plate (2) 180 degrees, so that the experimental mouse is placed on the limiting fixing plate (2) in a supine position. Place the head of the experimental mouse on the head holder (41), and place the mouth and nose of the experimental mouse at the position of the oxygen supply mimic hood (42). Fill the inner side of the oxygen supply tube (43) with anesthetic cotton balls, and use the oxygen supply mimic hood (42) to continuously anesthetize the experimental mouse with oxygen. Then place the limbs of the anesthetized experimental mouse at the corresponding positions of the limb fixing plate (21), and place the wrist of the experimental mouse at the end position of the limb restraint tube (22). , from the position of the tail placement slot (25) on the limiting fixing plate (2), the fixer cover plate (3) is buckled on the top of the limiting fixing plate (2), so that the fixer cover plate (3) is fixed to the head fixer (4) and the limiting fixing plate (2), wherein the limb fixing cover (31) and the limb restraint tube 2 (32) are buckled with the limb fixing plate (21) and the limb restraint tube 1 (22) respectively, and the limbs of the experimental mouse are pulled up so that the wrist of the experimental mouse completely passes through the limiting space of the limb restraint tube 1 (22) and the limb restraint tube 2 (32), and the straps (5) are respectively mounted on the limb restraint tube 1 (22) and the limb restraint tube 2 (32) at the positions corresponding to the wrist bones of the experimental mouse, so as to complete the fixation of the limbs of the experimental mouse, and then the tail of the experimental mouse is bent and placed on the outside of the limiting fixing plate (2); S3: Experimental operation, buckle the fixing column (13) on the radiation shielding cover (1) at the position of the positioning hole (34) on the fixing cover (3), complete the installation of the radiation shielding cover (1), then place the experimental mouse according to the irradiation position of the X-ray irradiator standard sample chamber, and choose to add the long plate shielding cover (12) or the short plate shielding cover (11) to the slide position of the radiation shielding cover (1), fix the irradiated experimental mouse, close the X-ray irradiator standard sample chamber door, and carry out the irradiation experiment; S4: Release the fixation. After the irradiation experiment is completed, open the door of the sample chamber of the X-ray irradiator, move the experimental mouse covered with the radiation shielding cover (1) to the experimental table, then move the fixing column (13) on the radiation shielding cover (1) and the positioning hole (34) on the fixer cover (3) upward and separate them, remove the strap (5) from the fixed position of the limb restraint tube (22) and the neck pressure frame (33), and throw them into the waste collection bin, move the limit fixing plate (2) and the fixer cover (3) upward and separate them, then separate the head fixer (4) from the limit fixing plate (2), and remove the irradiated experimental mouse; S5: Cleaning and disinfection: disassemble the above-mentioned experimental mouse holder, rinse the parts with clean water to remove residual stains and hair, then soak them in disinfectant water for 15 minutes, take them out and dry them for storage.

10. The method for using the laboratory mouse holder adapted for an X-ray irradiator according to claim 9, characterized in that: When collecting the lung lobe tissue of the above-mentioned experimental mouse, the above-mentioned experimental mouse is placed in a sterile surgical environment, and the above-mentioned steps S1 and S2 are repeated to fix the irradiated experimental mouse; The spreader (6) is fixed to the position of the positioning plate (7) and adjusted for standby use. When the incision of the experimental mouse is exposed, the support hooks (63) are buckled and fixed to the support incisions on both sides of the incision of the experimental mouse. After the sampling is completed, the support hooks (63) are removed from the incision position, and the incision of the experimental mouse is disinfected and sutured. The spreader (6) is removed from the position of the positioning plate (7), and the steps of removing the experimental mouse from the fixture in S4 are repeated. Then, the steps of S5 are repeated to disinfect the above-mentioned experimental mouse fixture, and the disinfection and storage are completed.

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