Sandwich structure composite rotator cuff patch induced tendon regeneration method

Abstract

The invention discloses a method for inducing tendon regeneration by using a sandwich-structured composite rotator cuff patch, which comprises the following implementation steps: step 1, weighing a certain amount of gelatin by using an electronic scale, and preparing a 12% wt mixed solution by using hexafluoroisopropanol as a solvent; 2, magnetically stirring the mixed solution in the step 1 for 24 hours, pouring the mixed solution into a 10 mL injector, and performing electrostatic spinning, in the process, setting the spinning voltage to be 18 kV, the propelling speed to be 1.2 mm / h, the temperature to be 25 DEG C and the humidity to be 60%; step 3; and based on the electrostatic spinning in the step 2, carrying out electrostatic spinning by using a 20G needle. And fixing the PET woven fabric on an orientation receiver for electrostatic spinning, after the composite spinning film is completely attached to the PET woven fabric, performing electrostatic spinning on the other side according to the same steps, and performing a microstructure test by using an SEM (Scanning Electron Microscope). Compared with the prior art, the patch disclosed by the invention has the advantages that the patch has double effects, namely a biological membrane has high orientation and can induce tendon oriented growth and promote regeneration of new tissues, and PET woven cloth has high mechanical properties and provides mechanical support for regeneration of the new tissues.

Description

Technical Field

[0001] The invention relates to the technical field of rotator cuff patches, in particular to a method for inducing tendon regeneration with a sandwich structure composite rotator cuff patch. Background Art

[0002] The nanoscale electrospun membrane has a structure similar to that of the extracellular matrix, which can promote the generation of new compensatory repair tissue and the reconstruction of damaged tendons. The process of rotator cuff reconstruction is the process of scar tissue reconstruction along the rotator cuff patch. As the rotator cuff patch degrades, the mechanical strength provided by the rotator cuff patch is gradually replaced by the new scar tissue, ultimately achieving the rehabilitation purpose of functional reconstruction.

[0003] Polycaprolactone (PCL) is a synthetic biodegradable polymer with good biocompatibility and biodegradability. It has a slow degradation rate, less acid decomposition products, and has the potential for load-bearing applications. PCL also has good mechanical properties, high flexibility and large stretchability. PCL is a hydrophobic polymer material with low biological activity, lacks cell adhesion sites, and has no ability to induce tissue regeneration, so it needs to be synergistic with other bioactive materials to improve its application performance. Summary of the invention

[0004] The technical problem to be solved by the present invention is to overcome the above technical defects and provide a lighting device that can be easily adjusted, can be easily moved and can be stably fixed on the ground and can provide protection.

[0005] In order to solve the above problems, the technical solution of the present invention is: a method for inducing tendon regeneration by a sandwich structure composite rotator cuff patch, comprising the following implementation steps:

[0006] Step 1: Use an electronic balance to weigh a certain amount of gelatin, and use hexafluoroisopropanol as a solvent to prepare a 12% wt mixed solution;

[0007] Step 2: The mixed solution in step 1 was stirred magnetically for 24 hours, and the mixed solution was poured into a 10 mL syringe and subjected to electrospinning. During the process, the spinning voltage was set to 18 kV, the propulsion speed was set to 1.2 mm / h, the temperature was set to 25° C., and the humidity was set to 60%;

[0008] Step 3: Based on the electrospinning in step 2, use a 20G needle to perform electrospinning. Fix the PET fabric on the orientation receiver for electrospinning. After the composite spinning membrane is completely attached to the PET fabric, perform electrospinning on the other side according to the same steps, and use SEM for microscopic morphology testing.

[0009] Furthermore, based on step 2, after the electrospinning is completed, the electrospinning membrane is embedded in the PET fabric using hot pressing to fix it, and then electrospinning is performed again. After reaching a certain thickness, the same operation steps are performed on the other side.

[0010] Furthermore, the spinning voltage in the electrospinning parameters is 16-28 kV, preferably 18-20 kV.

[0011] Furthermore, the receiving distance in the electrospinning parameters is 10-20 cm, preferably 15-18 cm.

[0012] Furthermore, the receiver speed in the electrospinning parameters is 500-2000 rpm, preferably 800-1000 rpm.

[0013] Furthermore, the needle used in the electrospinning parameters is 19-22G, preferably 20-21G.

[0014] The advantages of the present invention compared with the prior art are:

[0015] The present invention provides a controllable degradable composite bionic multi-layer rotator cuff tear repair patch, which has high mechanical properties, orientation and porosity, can induce tendon regeneration, and provide mechanical protection at the same time. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1 The invention relates to woven PET fibers for the method of inducing tendon regeneration by a sandwich structure composite rotator cuff patch.

[0017] Figure 2 It is a SEM photograph of the electrospun composite fiber membrane of the sandwich structure composite rotator cuff patch inducing tendon regeneration method of the present invention. DETAILED DESCRIPTION

[0018] The specific implementation of the present invention is further described below in conjunction with the accompanying drawings, wherein the same components are represented by the same reference numerals.

[0019] It should be noted that the words "front", "rear", "left", "right", "up" and "down" used in the following description refer to directions in the drawings, and the words "inside" and "outside" refer to directions toward or away from the geometric center of a specific component, respectively.

[0020] In order to make the contents of the present invention more clearly understood, the technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the accompanying drawings in the embodiments of the present invention.

[0021] like Figure 1 to Figure 2 As shown, the method for inducing tendon regeneration with a sandwich structure composite rotator cuff patch includes the following implementation steps:

[0022] Step 1: Use an electronic balance to weigh a certain amount of gelatin, and use hexafluoroisopropanol as a solvent to prepare a 12% wt mixed solution;

[0023] Step 2: The mixed solution in step 1 was stirred magnetically for 24 hours, and the mixed solution was poured into a 10 mL syringe and subjected to electrospinning. During the process, the spinning voltage was set to 18 kV, the propulsion speed was set to 1.2 mm / h, the temperature was set to 25° C., and the humidity was set to 60%;

[0024] Step 3: Based on the electrospinning in step 2, use a 20G needle to perform electrospinning. Fix the PET fabric on the orientation receiver for electrospinning. After the composite spinning membrane is completely attached to the PET fabric, perform electrospinning on the other side according to the same steps, and use SEM for microscopic morphology testing.

[0025] Based on step 2, after the electrospinning is completed, the electrospinning membrane is embedded in the PET fabric using hot pressing to fix it, and then electrospinning is performed again. After reaching a certain thickness, the same operation steps are performed on the other side.

[0026] The spinning voltage in the electrospinning parameters is 16-28 kV, preferably 18-20 kV, the receiving distance in the electrospinning parameters is 10-20 cm, preferably 15-18 cm, the receiver speed in the electrospinning parameters is 500-2000 rpm, preferably 800-1000 rpm, and the needle used in the electrospinning parameters is 19-22 G, preferably 20-21 G.

[0027] The present invention and its embodiments are described above, and such description is not restrictive. The drawings show only one embodiment of the present invention, and the actual structure is not limited thereto. In short, if ordinary technicians in the field are inspired by it, without departing from the purpose of the invention, they can design a structure and embodiment similar to the technical solution without creativity, which should belong to the protection scope of the present invention.

Claims

1. A method for inducing tendon regeneration using a sandwich structure composite rotator cuff patch, comprising the following implementation steps: Step 1: Use an electronic balance to weigh a certain amount of gelatin, and use hexafluoroisopropanol as a solvent to prepare a 12% wt mixed solution; Step 2: The mixed solution in step 1 was stirred magnetically for 24 hours, and the mixed solution was poured into a 10 mL syringe and subjected to electrospinning. During the process, the spinning voltage was set to 18 kV, the propulsion speed was set to 1.2 mm / h, the temperature was set to 25° C., and the humidity was set to 60%; Step 3: Based on the electrospinning in step 2, use a 20G needle to perform electrospinning. Fix the PET fabric on the orientation receiver for electrospinning. After the composite spinning membrane is completely attached to the PET fabric, perform electrospinning on the other side according to the same steps, and use SEM for microscopic morphology testing.

2. The method for inducing tendon regeneration using a sandwich structure composite rotator cuff patch according to claim 1, characterized in that: Based on step 2, after the electrospinning is completed, the electrospinning membrane is embedded in the PET fabric using hot pressing to fix it, and then electrospinning is performed again. After reaching a certain thickness, the same operation steps are performed on the other side.

3. The method for inducing tendon regeneration using a sandwich structure composite rotator cuff patch according to claim 1, characterized in that: The spinning voltage in the electrospinning parameters is 16-28 kV, preferably 18-20 kV.

4. The method for inducing tendon regeneration using a sandwich structure composite rotator cuff patch according to claim 1, characterized in that: The receiving distance in the electrospinning parameters is 10-20 cm, preferably 15-18 cm.

5. The method for inducing tendon regeneration using a sandwich structure composite rotator cuff patch according to claim 1, characterized in that: The receiver speed in the electrospinning parameters is 500-2000 rpm, preferably 800-1000 rpm.

6. The method for inducing tendon regeneration using a sandwich structure composite rotator cuff patch according to claim 1, characterized in that: The needle used in the electrospinning parameters is 19-22G, preferably 20-21G.

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