Reaction zone unit of micro-fluidic chip and reflective condensation reading device

Abstract

The invention provides a micro-fluidic chip reaction zone unit. The micro-fluidic chip reaction zone unit comprises a chip substrate; the reflective body is formed on the chip substrate, an accommodating cavity for accommodating a sample reagent mixed solution is formed in the reflective body, and a light-transmitting area is arranged at the bottom of the reflective body; the chip runner is formed on the chip substrate, is communicated with the accommodating cavity and is used for allowing the reagent mixed solution to flow into the accommodating cavity; the condensing lens is arranged at the upper part of the reflecting body, and a reading area is formed at the upper end of the condensing lens; wherein the light reflecting body is constructed to enable light signals to be emitted from the light transmitting area to enter the sample reagent mixed liquid in the containing cavity, the light signals diffused to the periphery through the sample reagent mixed liquid are reflected through the inner wall face of the light reflecting body, the light signals are converged to the condensing lens, and the condensing lens can converge the converged light signals to the reading area. The invention also provides a reflection condensation reading device comprising the reaction zone unit.

Description

Technical Field

[0001] The invention belongs to the technical field of chemiluminescence analysis, and in particular relates to a reaction zone unit of a microfluidic chip, and a reflection focusing reading device comprising the reaction zone unit. Background Art

[0002] The excitation light generator and the photon reader of the existing optically excited photon reader are mostly in the same structure, and the excitation light and the reading light are generated in the same direction. The excitation light is first excited and then read by the reader. Since the reaction reagents are mostly planar liquids, they will reflect the excitation light to a certain extent, and the excitation light itself is difficult to completely penetrate the reaction liquid, there is a certain energy loss, and part of the reaction liquid does not absorb energy for excitation, so the generated light signal is relatively low. Moreover, the excitation light cannot be scattered evenly after entering the reaction liquid, resulting in some solutions in the reaction liquid not being excited and being excited at different times, which reduces the final light signal peak. In addition, because the reader is on the same side of the excitation light generator, it is necessary to wait for a period of time after the excitation light generator is turned off before reading. This requires precise control of the interval time, which makes it easy to be interfered by the excitation light generator and may miss the best photon reading time.

[0003] In addition, the reaction zone structure of the existing light-emitting microfluidic chip is mostly a single plane structure, and there is no specific three-dimensional structure to enhance the post-reaction reading signal. The general reaction zone structure is designed to promote the reaction process or provide a reaction site, and then the signal is simply read using an external reader. Common reaction zone structures are mostly based on planar structures or simple grooves. Due to the light-transmitting characteristics of microfluidic chip materials, the light signal of the conventional reaction zone structure is easy to disperse, easy to overflow, not aggregate, low reading efficiency, signal CV (coefficient of variation) is large, weak signals are not easy to read, and are greatly affected by the uniformity of the reaction zone reagents, the position of the excitation light source, and the reading position of the reading instrument. Most of the existing chips are transparent chips. In the reaction zone, the light signal is easy to diffuse around and is difficult to gather in one place. In addition, the flat reaction zone structure is not conducive to laser excitation, and the high-energy laser can only excite a smaller point surface, resulting in low excitation efficiency. Summary of the invention

[0004] In view of the above technical problems, the present invention aims to provide a reaction zone unit of a microfluidic chip and a reflective light-collecting reading device comprising the reaction zone unit. The reaction zone unit of the microfluidic chip and the reflective light-collecting reading device comprising the reaction zone unit can effectively improve the excitation efficiency and the collection efficiency of the light signal, which is conducive to improving the sensitivity of the light signal reading, improving the reading efficiency, and reducing the signal CV.

[0005] To this end, according to the first aspect of the present invention, there is provided a reaction zone unit for a microfluidic chip, comprising: a reflective body formed on a chip substrate of the microfluidic chip, a receiving cavity for receiving a sample reagent mixture formed inside the reflective body, and a light-transmitting area provided on the reflective body; a chip flow channel formed on the chip substrate and connected to the receiving cavity, for allowing the sample reagent mixture to flow into the receiving cavity; and a focusing lens provided at the first end of the reflective body, and a reading area formed on the focusing lens. The reflective body is configured to allow a light signal from an external excitation light generator to enter the receiving cavity containing the sample reagent mixture from the light-transmitting area, and to be reflected by the inner wall surface of the reflective body and then converged to the focusing lens, and the focusing lens is configured to converge the converged light signal to the reading area for reading by a photon reader.

[0006] In one embodiment, the light-transmitting area is disposed at a second end of the reflective body opposite to the first end.

[0007] In one embodiment, a reflective layer is provided on the inner wall surface of the reflective body for reflecting light signals.

[0008] In one embodiment, the cross-sectional shape of the reflective body is configured as a geometric polygon.

[0009] In one embodiment, an excitation light scattering lens is provided in the containing cavity, and the excitation light scattering lens is arranged at the light-transmitting area to uniformly scatter the light signal into the sample-reagent mixture in the containing cavity.

[0010] In one embodiment, the reflective body is configured as a reaction cup structure, and the reaction cup structure is configured as a bullet-shaped cup structure, wherein the first end of the reflective body is the end opposite to the bullet head portion of the reaction cup structure, and the light-transmitting area is arranged at the first end of the reflective body.

[0011] In one embodiment, an injection-molded reaction cup body is installed in the reflective body, and a side portion of the injection-molded reaction cup body is provided with an opening communicating with the chip flow channel.

[0012] In one embodiment, a reflective layer is provided on the inner wall surface of the reflective body for reflecting light signals.

[0013] In one embodiment, the reflective body and the chip flow channel are formed as one body with the chip substrate.

[0014] According to a second aspect of the present invention, a reflective focusing reading device is provided, comprising: a reaction zone unit for a microfluidic chip as described above; an excitation light generator for generating excitation light, the excitation light generator being capable of moving to the vicinity of the light-transmitting area so as to emit excitation light into the containing cavity through the light-transmitting area; and a photon reader, the photon reader being capable of moving to the vicinity of the reading area so as to read the light signal focused by the focusing lens.

[0015] Compared with the prior art, the advantages of this application are:

[0016] According to the reaction zone unit of the microfluidic chip of the present invention, by setting an excitation light scattering lens on the bottom layer of the reflective body, the excitation light can be scattered more evenly into each position of the sample reagent mixture, so that the peak value of the light signal generated by the reaction liquid is higher, and the time of generating the light signal is more synchronized, which is very convenient for the detection of the photon reader. The reaction zone unit of the microfluidic chip utilizes the combined effect of the reflective structure and the focusing structure, so that the light signal originally dispersed in the reflective body can be directed to converge to the photon reader to enhance the read light signal. In addition, the coating formed by the reflective material and the light-shielding material is very helpful to reduce the leakage of the light signal, reduce the reading CV, and improve the final reagent sensitivity. In addition, due to the use of physical reflection and focusing of the light signal, the amplification of the light signal is more stable, and the amplification result is determined by the reaction itself, without introducing other factors, and the result is more reliable. In addition, by using the reflective material and the light-shielding material, it is possible to prevent the light from being lost through the chip and to gather the light reflection, so that the capture of the light signal is more efficient.

[0017] According to the reflective focusing reading device of the present invention, the excitation light generator and the photon reader are not in the same structure, and the two are relatively distributed and not in the same direction, so as to avoid mutual interference between the excitation light generator and the photon reader, and allow the excitation light to fully penetrate the reaction liquid. In this way, the excitation light directly reaches the interior of the sample reagent mixture, avoiding the reflection of the excitation light by the surface of the sample reagent mixture, reducing the energy loss, and reducing the proportion of the reaction liquid that does not absorb energy, thereby being able to more fully excite the reaction liquid and greatly improving the light excitation efficiency. At the same time, the working interval between the excitation light generator and the photon reader can be reduced, shortening the overall working time. At the same time, penetrating excitation can more effectively excite the reaction liquid and generate more light signals. After the excitation light passes through the excitation light scattering lens, the excitation light is more evenly distributed in the sample reagent mixture, generating more synchronous light signals, and the signal peaks are more concentrated. In addition, the reflective material and the light-shielding material can both prevent the light from being lost through the chip and reflect the light and concentrate it, making the capture of the light signal more efficient. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] The present invention will be described below with reference to the accompanying drawings.

[0019] Figure 1 The structure of the first embodiment of the reaction zone unit of the microfluidic chip according to the present invention is schematically shown.

[0020] Figure 2 The structure of the second embodiment of the reaction zone unit of the microfluidic chip according to the present invention is schematically shown.

[0021] Figure 3 and Figure 4 The diagram schematically shows the states before and after the injection-molded reaction body is combined with the reaction cup in the second embodiment of the reaction zone unit of the microfluidic chip according to the present invention.

[0022] Figure 5 and Figure 6 The structure of an injection-molded reaction body with different volumes is schematically shown.

[0023] In the present application, all drawings are schematic drawings, which are only used to illustrate the principles of the present invention and are not necessarily drawn according to the actual scale. DETAILED DESCRIPTION

[0024] The present invention will be described below in conjunction with the accompanying drawings.

[0025] Figure 1 The structure of the reaction zone unit of the microfluidic chip according to the present invention is schematically shown. Figure 1 As shown, the reaction zone unit 100 of the microfluidic chip includes a reflective body 2 formed on a chip substrate 1, and a chip flow channel 11 formed on the chip substrate 1. A receiving cavity 20 for receiving a sample reagent mixture is formed inside the reflective body 2. In the illustrated embodiment, a light-transmitting area 21 is provided at the bottom of the reflective body 2. The chip flow channel 11 is connected to the receiving cavity 20 for introducing the sample reagent mixture into the receiving cavity 20.

[0026] like Figure 1 As shown, the microfluidic chip reaction zone unit 100 also includes a condenser lens 3 disposed on the upper part of the reflective body 2, and a reading area 31 is formed at the upper end of the condenser lens 3. In the present application, it should be noted that the directional terms or qualifiers such as "upper" and "lower" used in the present application are all for the referenced drawings, and are only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as a limitation to the present invention.

[0027] According to the present invention, the reflective body 2 is configured to allow the light signal to enter the containing cavity 20 from the light-transmitting area 21, and at the same time, the light signal that is dispersed around the sample reagent mixture is reflected by the inner wall surface of the reflective body 2, so that the light signal is gathered to the condenser lens 3. Therefore, the condenser lens 3 can gather the gathered light signal to the reading area 31.

[0028] According to the present invention, a reflective layer (not shown) is provided on the inner wall surface of the reflective body 2 for reflecting light signals.

[0029] The reflective body 2 and the chip flow channel 11 are etched on the chip substrate 1 and are directly formed without a specific connection method, thereby forming a whole with the chip substrate 1.

[0030] According to the first embodiment of the present invention, Figure 1 As shown, the cross-sectional shape of the reflective body 2 is configured as a geometric polygon, which may be, for example, a hexagon, an octagon or other shapes.

[0031] Preferably, an excitation light scattering lens 22 may be provided in the accommodating cavity 20. The excitation light scattering lens 22 is disposed at the position of the light-transmitting region 21, and is used to evenly scatter the light signal into the sample reagent mixture. The excitation light scattering lens 22 is configured to match the shape of the excitation light generator 5 (see below). The excitation light scattering lens 22 allows the excitation light energy to be more evenly scattered into various positions of the sample reagent mixture, so that the peak value of the light signal generated by the sample reagent mixture is higher, and the time of generating the light signal is more synchronized, which is very convenient for detection by the photon reader 4.

[0032] In one embodiment, the reflective layer is a first reflective coating (not shown) sprayed on the inner wall surface of the reflective body 2. The first reflective coating is formed by spraying a reflective material that does not react with the reagent. The first reflective coating is sprayed on the inner surface of the reflective body 2 except for the light-transmitting area 21 and the excitation light scattering lens 22. The first reflective coating can effectively reflect the light signal passing through the sample reagent mixture while ensuring that the light signal can pass through the light-transmitting area 21.

[0033] Except for the connection between the focusing lens 3 and the photon reader 4 (see below), the rest of the outer surface is sprayed with a light-shielding layer to prevent leakage of optical signals.

[0034] Preferably, the condenser lens 3 can be overlapped and bonded with the upper region of the chip substrate 1. Of course, the condenser lens 3 can also be directly integrated below the reading port of the photon reader 4, thereby forming a fixed condenser lens cover.

[0035] According to the microfluidic chip reaction zone unit 100 in the first embodiment of the present invention, when actually working, the sample reagent mixture flows into the containing cavity 20 through the chip flow channel 11. After sufficient mixing reaction, the excitation light generator 5 and the photon reader 4 are close to the chip and fit with the corresponding interface. Specifically, the excitation light generator 5 is close to the light-transmitting area 21 at the lower end of the reflective body 2, and the photon reader 4 is close to the reading area 31 at the upper end of the focusing lens 3. Afterwards, the excitation light generator 5 generates excitation light, which penetrates the light-transmitting area 21 at the lower end of the reflective body 2, and is evenly scattered into the sample reagent mixture through the excitation light scattering lens 22, and excites the sample reagent mixture to generate a light signal. The light signal is emitted in all directions in the sample reagent mixture, and the light signals emitted downward and to the side are reflected by the first reflective coating on the inner wall surface of the reflective body 2, change direction and converge upward, and converge together with the light signal originally emitted upward through the focusing lens 3 and enter the photon reader 4. As a result, readings can be made through the photon reader 4.

[0036] In this embodiment, the microfluidic chip reaction zone unit 100 sets an excitation light scattering lens 22 on the bottom layer of the reflective body 2, so that the excitation light can be scattered more evenly into various positions of the sample reagent mixture, so that the peak value of the light signal generated by the reaction liquid is higher, and the time of generating the light signal is more synchronized, which is very convenient for the detection of the photon reader 4. The microfluidic chip reaction zone unit 100 uses the combined effect of the reflective structure and the focusing structure to enable the originally dispersed light signal in the reflective body 2 to be directed and converged to the photon reader 4 to enhance the read light signal. In addition, the coating formed by the reflective material and the light-shielding material is very helpful in reducing the leakage of the light signal, reducing the reading CV, and improving the final reagent sensitivity.

[0037] The reflection direction depends on the fixed reflection structure. By adjusting the structural angle, the focus of the reflected light can be controlled to adapt to different reaction area volumes and chip thicknesses. Different focusing lenses 3 can correspond to reading ports of different sizes of the photon reader 4, and can be freely replaced and adjusted, with high controllability. In addition, due to the use of physical reflection and focusing of light signals, the amplification of light signals is more stable, and the amplification result is determined by the reaction itself, without introducing other factors, and the result is more reliable. In addition, by using reflective materials and light-shielding materials, it is possible to prevent light from being lost through the chip and to concentrate light reflections, making the capture of light signals more efficient.

[0038] Figure 2The second embodiment of the present invention is shown. The difference between the second embodiment and the first embodiment lies in the reflective body 2. In the second embodiment, the reflective body 2' is configured as a reaction cup structure, and the reaction cup structure is configured as a bullet-like cup structure. Specifically, the reaction cup is a bullet-like cup structure etched on the chip substrate 1, and its bottom is shaped like a bullet head, which can gather the sample reagent mixture at the bottom. The inner cavity of the reaction cup is formed as a receiving cavity 20', and the cup body is slender, so that the sample reagent mixture can form a sufficient height.

[0039] In one embodiment, the reflective layer can be, for example, a second reflective coating (not shown) sprayed on the inner wall of the reaction cup to form a reflective cup wall. The second reflective coating is formed by spraying a reflective material that does not react with the sample reagent mixture and can effectively reflect light signals scattered by the sample reagent mixture.

[0040] In another embodiment, Figure 3 and Figure 4 As shown, the reflective layer is configured as an injection molded reaction cup body 6 that can be adapted to the reaction cup. The injection molded reaction cup body 6 is formed by injection molding and placed in the reaction cup. The side of the injection molded reaction cup body 6 is provided with an opening 61 that is connected to the chip flow channel 11. Thus, the injection molded reaction cup body 6, as a reflective cup wall, can effectively reflect the light signal scattered by the sample reagent mixture.

[0041] The upper end of the reflective body 2' is provided with a light-transmitting area (not shown) for stimulating the light generator ( Figure 2 The specific configuration and structure of the excitation light generator used in the second embodiment may refer to the prior patent CN212622236U of the same applicant, which is incorporated herein by reference in its entirety.

[0042] Similarly, the condenser lens 3 is light-transmissive except for the connection with the photon reader 4 (see below), and the rest of the outer surface is sprayed with a light-shielding layer to prevent leakage of light signals. Preferably, the condenser lens 3 can be overlapped and bonded with the upper area of ​​the chip substrate 1. Of course, the condenser lens 3 can also be directly integrated below the reading port of the photon reader 4, thereby forming a fixed condenser lens cover.

[0043] like Figure 5 and Figure 6 As shown, the injection molded reaction cup body 6 is made of injection molding material and can be formed into different sizes, heights, and thicknesses. Its bottom matches the reaction cup and can be appropriately configured in the reaction cup. The internal space of the injection molded reaction cup body 6 can freely adjust the thickness to accommodate different volumes of sample reagent mixtures. Figure 5 and Figure 6Injection-molded reaction cup bodies 6 with different thicknesses are schematically shown, and the sides of the reaction cup bodies 6 are respectively provided with openings 61 for communicating with the chip flow channel 11 .

[0044] According to the microfluidic chip reaction zone unit 100' in the second embodiment of the present invention, when actually working, the sample reagent mixture flows into the containing cavity 20' through the chip flow channel 11. After sufficient mixing reaction, the excitation light generator and the photon reader 4 are close to the chip and fit with the corresponding interface. Specifically, the excitation light generator is close to the light-transmitting area at the upper end of the reflective body 2, and the photon reader 4 is close to the reading area 31 at the upper end of the focusing lens 3. Afterwards, the excitation light generator generates excitation light, and the excitation light penetrates the light-transmitting area on the reflective body 2' and enters the sample reagent mixture, exciting the sample reagent mixture to generate a light signal. The light signal is emitted in all directions in the sample reagent mixture, and the light signals emitted downward and to the sides are reflected by the reflective cup wall of the bullet-shaped reaction cup, change direction and converge upward, and converge together with the light signal originally emitted upward through the focusing lens 3 and enter the photon reader 4. As a result, readings can be taken through the photon reader 4.

[0045] In practical applications, the sizes of different reaction areas can be controlled by inserting injection molded reaction cup bodies 6 of different sizes into the reaction cups. The size of the reaction area can be adjusted by the different sizes of the reaction cups, so that different volumes of liquid can form different liquid level heights to match the optimal excitation light emission position and reading position. Different focusing lenses 3 can be freely adjusted and replaced to adapt to different photon readers 4. This significantly improves the adjustability of the microfluidic chip reaction area unit 100.

[0046] In this embodiment, the microfluidic chip reaction zone unit 100' utilizes the combined effect of the reflective structure and the focusing structure to enable the originally dispersed light signals in the reflective body 2' to be directed and converged to the photon reader 4 to enhance the read light signals. In addition, the reflective layer is made of opaque reflective material or directly etched from the chip and then sprayed, which is very helpful to reduce the leakage of light signals, enhance the reflection of light signals, and increase sensitivity.

[0047] The reaction cup is formed by a deeper cup-shaped structure, and its depth is deeper, which allows the sample reagent mixture to form a certain thickness inside. Therefore, by deepening the depth of the reaction cup to increase the optical path of laser excitation and increase the light reflection surface on the reaction cup wall, the excitation efficiency and the collection efficiency of the light signal can be effectively improved, which helps to further improve the sensitivity of the light signal reading, improve the reading efficiency, and reduce the signal CV.

[0048] In addition, due to the use of physical reflection and focusing of light signals, the amplification of light signals is more stable, and the amplification result is determined by the reaction itself without introducing other factors, so the result is more reliable. In addition, reflective materials and light-shielding materials can prevent light from being lost through the chip and can also focus light reflection, making the capture of light signals more efficient.

[0049] The present invention also provides a reflection focusing reading device. Figure 1 As shown, the reflective focusing reading device includes the microfluidic chip reaction area unit 100 as described above, an excitation light generator 5 for generating excitation light, and a photon reader 4. The excitation light generator 5 can be moved to the light-transmitting area 21 to emit the excitation light into the accommodating cavity 20 through the light-transmitting area 21. The photon reader 4 can be moved to the reading area 31 so that the light signal converged by the focusing lens 3 can enter the photon reader 4, and then read by the photon reader 4.

[0050] According to the present invention, the excitation light generator 5 and the photon reader 4 of the reflective focusing reading device are not in the same structure, and the two are relatively distributed and not in the same direction, thereby avoiding mutual interference between the excitation light generator 5 and the photon reader 4, and enabling the excitation light to fully penetrate the reaction liquid. In this way, the excitation light directly reaches the interior of the sample reagent mixture, avoiding the reflection of the excitation light by the surface of the sample reagent mixture, reducing energy loss, and reducing the proportion of unabsorbed energy in the reaction liquid, thereby being able to more fully excite the reaction liquid and greatly improving the light excitation efficiency. At the same time, the working interval between the excitation light generator 5 and the photon reader 4 can be reduced, shortening the overall working time.

[0051] At the same time, penetrating excitation can more effectively excite the reaction solution and generate more light signals. After the excitation light passes through the excitation light scattering lens 22, it is more evenly distributed in the sample reagent mixture, the light signal generated is more synchronous, the signal peak is more concentrated, and the reflective material and the light-shielding material can not only prevent the light from being lost through the chip, but also reflect and concentrate the light, making the capture of light signals more efficient.

[0052] In the description of the present invention, it should be understood that the terms "first" and "second" are used for descriptive purposes only and should not be understood as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include one or more of the features. In the description of the present invention, the meaning of "plurality" is two or more, unless otherwise clearly and specifically defined.

[0053] In addition, in the description of this specification, the description with reference to the terms "one embodiment", "some embodiments", "example", "specific example", or "some examples" means that the specific features, structures, materials or characteristics described in conjunction with the embodiment or example are included in at least one embodiment or example of the present invention. In this specification, the schematic representation of the above terms does not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials or characteristics described may be combined in any one or more embodiments or examples in a suitable manner.

[0054] Finally, it should be noted that the above is only a preferred embodiment of the present invention and does not constitute any limitation to the present invention. Although the present invention has been described in detail with reference to the aforementioned embodiments, it is still possible for a person skilled in the art to modify the technical solutions described in the aforementioned embodiments, or to replace some of the technical features therein by equivalents. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention shall be included in the protection scope of the present invention.

Claims

1. The reaction zone unit of the microfluidic chip comprises: A reflective body (2) formed on the chip substrate (1) of the microfluidic chip, wherein a receiving cavity (20) for receiving a sample reagent mixture is formed inside the reflective body (2), and a light-transmitting area (21) is provided on the reflective body (2); a chip flow channel (11) formed on the chip substrate (1) and connected to the receiving chamber (20), for allowing a sample reagent mixture to flow into the receiving chamber (20); and A condenser lens (3) disposed at a first end of the reflective body (2), wherein a reading area (31) is formed on the condenser lens (3); The reflective body (2) is configured to allow a light signal from an external excitation light generator (5) to enter a containing cavity (20) containing the sample reagent mixture from the light-transmitting area (21), and to be reflected by the inner wall surface of the reflective body (2) and then be collected by the focusing lens (3). The focusing lens (3) is configured to collect the collected light signals to the reading area (31) for reading by the photon reader (4).

2. The reaction zone unit of the microfluidic chip according to claim 1, characterized in that: The light-transmitting area (21) is arranged at a second end of the reflective body (2) opposite to the first end.

3. The reaction zone unit of the microfluidic chip according to claim 2, characterized in that: A reflective layer is provided on the inner wall surface of the reflective body (2) for reflecting light signals.

4. The reaction zone unit of the microfluidic chip according to claim 3, characterized in that: The cross-sectional shape of the reflective body (2) is configured as a geometric polygon.

5. The reaction zone unit of the microfluidic chip according to claim 4, characterized in that: An excitation light scattering lens (22) is provided in the containing cavity (20); the excitation light scattering lens (22) is arranged at the light-transmitting area (21) and is used to evenly scatter light signals into the sample reagent mixture in the containing cavity (20).

6. The reaction zone unit of the microfluidic chip according to claim 1, characterized in that: The reflective body (2') is configured as a reaction cup structure, and the reaction cup structure is configured as a bullet-shaped cup structure, wherein the first end of the reflective body (2') is the end opposite to the bullet head portion of the reaction cup structure, and the light-transmitting area is arranged at the first end of the reflective body (2').

7. The reaction zone unit of the microfluidic chip according to claim 6, characterized in that: An injection-molded reaction cup body (6) is installed in the reflective body (2'), and a side portion of the injection-molded reaction cup body (6) is provided with an opening (61) communicating with the chip flow channel (11).

8. The reaction zone unit of the microfluidic chip according to claim 6, characterized in that: A reflective layer is provided on the inner wall surface of the reflective body (2') for reflecting light signals.

9. The reaction zone unit of the microfluidic chip according to any one of claims 1 to 8, characterized in that: The reflective body (2) and the chip flow channel (11) are formed as one body with the chip substrate (1).

10. Reflection focusing reading device, comprising: The reaction zone unit (100) of the microfluidic chip according to any one of claims 1 to 9; an excitation light generator (5) for generating excitation light, wherein the excitation light generator (5) is movable to the vicinity of the light-transmitting area (21) so as to emit the excitation light into the accommodating cavity (20) through the light-transmitting area (21); as well as A photon reader (4) is capable of moving to the vicinity of the reading area (31) so as to read the light signal gathered by the condenser lens (3).

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