Antioxidant composition and application thereof in cosmetics

Abstract

The invention discloses an antioxidant composition and application thereof in cosmetics. The invention relates to an antioxidant composition which comprises quercetin and carnosine, the weight ratio of the quercetin to the carnosine is (1: 0.02)-(1: 400). The antioxidant composition provided by the invention is prepared from quercetin and carnosine, the weight ratio of the quercetin to the carnosine is (1: 0.02)-(1: 400). The anti-oxidation composition provided by the invention simultaneously contains the quercetin and the carnosine with a set amount, and the quercetin and the carnosine have a good synergistic anti-oxidation effect, so that the composition has good anti-oxidation capability, can be used as an anti-oxidation active component in the process of preparing cosmetics, and helps a user to delay the skin aging process.

Description

Technical Field

[0001] The present invention relates to the technical field of cosmetics, and in particular to an antioxidant composition and application thereof in cosmetics. Background Art

[0002] The skin is a tissue with high metabolic activity, but ultraviolet rays and polluted air in the external environment can induce the skin to produce harmful reactive oxygen species (ROS), which can cause oxidative damage to various biological macromolecules such as cell membranes, lipids, proteins, RNA, DNA, etc., and then destroy cells and increase the chance of DNA damage and mutation. When the reactive oxygen produced by the skin exceeds the capacity of the skin's own antioxidant defense system, the skin will experience oxidative stress.

[0003] Reactive oxygen species (ROS) are secondary metabolites produced by many metabolic pathways in the human body. At the same time, the human body will continue to produce reactive oxygen species when exposed to ultraviolet light and / or inflammation. The oxidative stress response of the skin is caused by an imbalance between oxidants and antioxidants in the skin. If the skin produces too much reactive oxygen species (ROS), or the antioxidants in the skin are insufficient, or the antioxidant cell defense system in the skin is reduced, it will cause damage to important processes such as skin cell replication, transcription and translation, leading to skin cell death and rapid skin aging.

[0004] Oxidative stress in the skin is generally caused by skin aging. Antioxidants can terminate the transmission of the oxidation chain in the oxidative stress reaction by combining with free radicals, making it impossible for free radicals to attack the biomacromolecules in the skin cells, thereby reducing the oxidative stress reaction in the skin.

[0005] However, among the numerous cosmetics in the prior art, there are not many products that can truly achieve anti-oxidation, reduce the oxidative stress response of the skin, and thus protect the skin and delay skin disease.

[0006] In view of this, it is indeed necessary to provide a technical solution to the above problems. Summary of the invention

[0007] The purpose of the present invention is to provide an antioxidant composition with good antioxidant performance which can be used as an antioxidant active ingredient in cosmetics in view of the deficiencies of the prior art.

[0008] In order to achieve the above object, the present invention provides an antioxidant composition, comprising quercetin and carnosine; the weight ratio of quercetin to carnosine is 1:0.02 to 1:400.

[0009] In some embodiments of the antioxidant composition, the weight ratio of quercetin to carnosine is 1:1 to 1:400.

[0010] In some embodiments of the antioxidant composition, the weight ratio of quercetin to carnosine is 1:10 to 1:100.

[0011] In order to achieve the above-mentioned purpose, the present invention also provides a cosmetic, comprising quercetin and carnosine, wherein the weight ratio of quercetin to carnosine is 1:0.02 to 1:400; the content of the quercetin in the cosmetic is in the range of 0.01 to 1% by weight, and the content of the carnosine in the cosmetic is in the range of 0.01 to 4% by weight.

[0012] In the cosmetics described in some embodiments, the content of quercetin in the cosmetics ranges from 0.01 to 0.5% by weight, and the content of carnosine in the cosmetics ranges from 0.1 to 4% by weight.

[0013] In the cosmetics described in some embodiments, the content of quercetin in the cosmetics ranges from 0.01 to 0.2% by weight, and the content of carnosine in the cosmetics ranges from 0.5 to 4% by weight.

[0014] In order to achieve the above object, the present invention also provides the use of the antioxidant composition as an antioxidant active ingredient in cosmetics.

[0015] The beneficial effects of the present invention are:

[0016] The antioxidant composition provided by the present invention comprises quercetin and carnosine; the weight ratio of quercetin to carnosine is 1:0.02 to 1:400. The antioxidant composition provided by the present invention comprises a set amount of quercetin and carnosine at the same time, and the two have a good synergistic antioxidant effect, so that the composition has good antioxidant capacity, and can be used as an antioxidant active ingredient in the process of preparing cosmetics, helping users to delay the aging process of the skin. DETAILED DESCRIPTION

[0017] To make the technical solutions and advantages of the present invention more clear, the present invention and its beneficial effects will be further described in detail below in conjunction with specific implementation methods, but the implementation methods of the present invention are not limited thereto.

[0018] In order to achieve the above object, the present invention adopts the following technical solutions:

[0019] An antioxidant composition comprises quercetin and carnosine; the weight ratio of quercetin to carnosine is 1:0.02-1:400.

[0020] In some embodiments of the antioxidant composition, the weight ratio of quercetin to carnosine is 1:1 to 1:400.

[0021] In some embodiments of the antioxidant composition, the weight ratio of quercetin to carnosine is 1:10 to 1:100.

[0022] In order to achieve the above-mentioned purpose, the present invention also provides a cosmetic, comprising quercetin and carnosine, wherein the weight ratio of quercetin to carnosine is 1:0.02 to 1:400; the content of the quercetin in the cosmetic is in the range of 0.01 to 1% by weight, and the content of the carnosine in the cosmetic is in the range of 0.01 to 4% by weight.

[0023] In the cosmetics described in some embodiments, the content of quercetin in the cosmetics ranges from 0.01 to 0.5% by weight, and the content of carnosine in the cosmetics ranges from 0.1 to 4% by weight.

[0024] In the cosmetics described in some embodiments, the content of quercetin in the cosmetics ranges from 0.01 to 0.2% by weight, and the content of carnosine in the cosmetics ranges from 0.5 to 4% by weight.

[0025] In order to achieve the above object, the present invention also provides the use of the antioxidant composition as an antioxidant active ingredient in cosmetics.

[0026] Embodiment 1:

[0027] 0.01 g of quercetin and 0.01 g of carnosine were mixed at room temperature and pressure to obtain the antioxidant composition of Example 1. The antioxidant composition was added to 99.98 g of phosphate buffered saline to form a mixed solution. The mixed solution was heated to completely dissolve the mixed solution at 80° C. to obtain an antioxidant composition solution with a quercetin content of 0.01% and a carnosine content of 0.01%.

[0028] The preparation process of the phosphate buffered saline solution is as follows: 8 grams of NaCl, 0.2 grams of KCl, 1.44 grams of Na2HPO4, and 0.24 grams of KH2PO4 are weighed and placed in a 1L container. 800 ml of deionized water is added to the container and stirred for 5 minutes to completely dissolve the components to obtain a mixed solution. The pH value of the mixed solution is measured. If the pH value is not 7.4, Na2HPO4 and / or KH2PO4 are used to fine-tune until the pH value is 7.4. Then deionized water is added to make the volume 1L, and the phosphate buffered saline solution required for Example 1 is obtained.

[0029] The oxidative composition prepared in Example 1 was tested for its oxidative free radical absorption capacity (ORAC)

[0030] The Oxygen Radical Absorbance Capacity (ORAC) test method is one of the most commonly used methods for detecting the antioxidant capacity of antioxidants. The principle is that a free radical initiator such as an azo initiator compound 2,2-azobis(2,-methylpropylimidazole) dihydrochloride is mixed with a fluorescent probe (fluorescein sodium) to destroy the fluorescent probe, causing a change in fluorescence intensity, and the fluorescence decay is quantified by using a fluorometer. The free radical initiator produces peroxy radicals. In the absence of an inhibitor, the free radicals will quickly destroy the fluorescence of the fluorescein dye. By tracking the time course of fluorescence decay and comparing the addition and non-addition of the test substance, the free radical capture capacity of the test substance can be estimated. Trolox, a water-soluble analog of vitamin E, is used as a quantitative standard, i.e., a standard reference substance, and a microplate fluorescence and chemiluminescence analyzer is used for measurement and analysis to measure the relative degree of protection of the sample and the standard reference substance, and the results are expressed in μMol equivalents of the standard reference substance.

[0031] The specific test method is as follows:

[0032] Take 25 μL of the antioxidant composition solution prepared in Example 1 as a sample and add it to a 96-well plate, and take 25 μL of the standard reference substance and add it to a 96-well plate. Add 150 μL of fluorescein solution to the sample and the standard reference substance respectively, and mix the fluorescein solution with the corresponding sample and the standard reference substance; then put the 96-well plate into a 37°C incubator and incubate for 30 minutes. Then add 25 μL of free radical initiator solution to the sample and the standard reference substance respectively and mix them evenly; finally, put the 96-well plate into a microplate fluorescence and chemiluminescence analyzer, and measure the fluorescence intensity of the sample and the standard reference substance under the conditions of an excitation light wavelength of 485 nm, an emission light wavelength of 538 nm, and a measurement temperature of 37°C. The measurement time is 60 min, and the interval is once every 1 min. The 96-well plate, standard reference substance, fluorescein solution, and free radical initiator solution used above were from the ORAC Assay kit (ab233473) of Abcam; the microplate fluorescence and chemiluminescence analyzer used was purchased from ThermoScientific's Fluoroskan FL microplate fluorescence and chemiluminescence analyzer.

[0033] Based on the 60 fluorescence intensity data of the sample measured in chronological order and the 60 fluorescence intensity data of the standard reference substance, the oxygen free radical absorption capacity of the sample and the standard reference substance is calculated. The oxygen free radical absorption capacity calculated in this step is the actual measured value of the oxygen free radical absorption capacity of the sample and the actual measured value of the oxygen free radical absorption capacity of the standard reference substance.

[0034] Table 1 shows the content parameters of each group of antioxidant composition solutions prepared in Examples 1 to 47, the ratio parameters between the components, and the component content parameters of Comparative Examples 1 to 15.

[0035]

[0036]

[0037]

[0038] Examples 2 to 47

[0039] The steps of Example 1 were repeated according to the content parameters of each component and the ratio parameters between the components specified in Examples 2 to 47 in Table 1. See Table 1 for specific parameters and test results.

[0040] Comparative Examples 1 to 15

[0041] The steps of Example 1 were repeated according to the component content parameters specified in Comparative Examples 1 to 15 in Table 1. See Table 1 for specific parameters and test results.

[0042] The measured data of Comparative Examples 1-6 are: the actual measured values ​​of oxygen free radical absorption capacity of quercetin samples of corresponding concentrations. The measured data of Comparative Examples 7-15 are: the actual measured values ​​of oxygen free radical absorption capacity of carnosine samples of corresponding concentrations.

[0043] The predicted value of the oxygen free radical absorption capacity of the antioxidant composition prepared in Examples 1-47 is: the measured value of the oxygen free radical absorption capacity of the quercetin sample of the corresponding concentration in the corresponding composition + the measured value of the oxygen free radical absorption capacity of the carnosine sample of the corresponding concentration in the corresponding composition.

[0044] The predicted values ​​and measured values ​​of the oxygen free radical absorption capacity of the antioxidant compositions prepared in Examples 1-47, the measured values ​​of the oxygen free radical absorption capacity of the quercetin samples corresponding to Comparative Examples 1-6, and the measured values ​​of the oxygen free radical absorption capacity of the carnosine samples corresponding to Comparative Examples 7-15 are specifically shown in Table 1.

[0045] From the predicted values ​​and measured values ​​of the oxygen free radical absorption capacity of the corresponding samples of Examples 1-47 shown in Table 1, and the measured values ​​of the oxygen free radical absorption capacity of the corresponding samples of Comparative Examples 1-15, it can be seen that the measured values ​​of ORAC of the antioxidant composition provided by the embodiments of the present invention are all increased by more than 20% compared with the corresponding predicted values, and most of them are more than 100%, indicating that the active ingredients quercetin and carnosine in the antioxidant composition provided by the embodiments of the present invention have synergistic antioxidant activity. From the specific data in Table 1, it can be seen that the measured values ​​of ORAC of more than 50% of the antioxidant composition provided by the embodiments of the present invention are increased by more than 500% compared with the corresponding predicted values. For example, the antioxidant composition solution prepared in Example 13 contains 0.05% quercetin and 0.5% carnosine, and the measured ORAC value obtained by the sample measurement is increased by 1955.08% compared with the predicted value, which indicates that the solution of the antioxidant composition of 0.05% quercetin and 0.5% carnosine provided in Example 13 can produce significant synergistic antioxidant effects. When the antioxidant composition is used as an antioxidant active ingredient in cosmetics, for example, when the cosmetics contain 0.05% by weight of quercetin and 0.5% by weight of carnosine, the quercetin and carnosine have the same synergistic antioxidant effect.

[0046] In summary, the antioxidant composition provided by the present invention comprises quercetin and carnosine; the weight ratio of quercetin to carnosine is 1:0.02 to 1:400; because the antioxidant composition comprises a set amount of quercetin and carnosine at the same time, the two have a good synergistic antioxidant effect, so that the antioxidant composition has good antioxidant capacity, and can be used as an antioxidant active ingredient in the process of preparing cosmetics to help users delay the aging process of the skin.

[0047] When the quercetin content and carnosine content of the cosmetics provided by the present invention are consistent with the content in the antioxidant composition solution prepared by a certain embodiment above, the ORAC oxygen free radical absorption capacity of the cosmetics and the corresponding antioxidant composition solution is the same, because the ORAC oxygen free radical absorption capacity of the two is synergistically produced by the corresponding content of quercetin and the corresponding content of carnosine. The solvent in the antioxidant composition solution and other ingredients in the cosmetics have no effect on the antioxidant synergistic effect of quercetin and carnosine. Therefore, the ORAC oxygen free radical absorption capacity of the antioxidant composition solution prepared by the above embodiments 1-47 is equivalent to the ORAC oxygen free radical absorption capacity of the cosmetics containing the corresponding content of quercetin and carnosine, and the corresponding embodiments and the corresponding ORAC oxygen free radical absorption capacity test methods and test results are no longer provided for the cosmetics of the present invention.

[0048] Those skilled in the art may combine and associate different embodiments or examples and features of different embodiments or examples described in this specification without mutual contradiction.

[0049] The above is only a specific embodiment of the present invention, but the protection scope of the present invention is not limited thereto. Any technician familiar with the technical field can easily think of various equivalent modifications or replacements within the technical scope disclosed by the present invention, and these modifications or replacements should be included in the protection scope of the present invention. Therefore, the protection scope of the present invention shall be based on the protection scope of the claims.

Claims

1. An antioxidant composition, characterized in that It includes quercetin and carnosine; the weight ratio of quercetin to carnosine is 1:0.02 to 1:

400.

2. The antioxidant composition according to claim 1, characterized in that The weight ratio of quercetin to carnosine is 1:1 to 1:

400.

3. The antioxidant composition according to claim 2, characterized in that The weight ratio of quercetin to carnosine is 1:10 to 1:

100.

4. A cosmetic, characterized in that: The cosmetic comprises quercetin and carnosine, wherein the weight ratio of quercetin to carnosine is 1:0.02 to 1:400; the content of the quercetin in the cosmetic is 0.01 to 1% by weight, and the content of the carnosine in the cosmetic is 0.01 to 4% by weight.

5. The cosmetic according to claim 4, characterized in that: The content of the quercetin in the cosmetics is in the range of 0.01-0.5% by weight, and the content of the carnosine in the cosmetics is in the range of 0.1-4% by weight.

6. The cosmetic according to claim 5, characterized in that: The content of the quercetin in the cosmetics is in the range of 0.01-0.2% by weight, and the content of the carnosine in the cosmetics is in the range of 0.5-4%.

7. Use of the antioxidant composition according to any one of claims 1 to 3 as an antioxidant active ingredient in cosmetics.

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