Full-automatic bacterium experiment equipment and use method thereof

Abstract

The invention discloses full-automatic bacterium experiment equipment and a use method thereof.The full-automatic bacterium experiment equipment comprises a box body, a partition plate is arranged in the middle of the box body, the box body is divided into two completely independent operation areas through the partition plate, one side is a bacterium cryopreservation box, and the other side is a bacterium operation box; two symmetrical sealing door devices are respectively arranged above and right in front of the box body, ultraviolet lamp devices are arranged on the inner sides of the symmetrical sealing door devices above the box body, a motor and a refrigerating and heating temperature control system device are arranged below the box body, an operation panel is arranged on the upper left portion of the box body, full-automatic bacteria operation software is arranged in the operation panel, and the upper left portion of the box body is provided with a control panel. The operation panel is connected to the computer through a panel line. The full-automatic bacterium experiment equipment can replace manual operation of bacteria, the full-automatic operation steps are simple and efficient, the problem of bacterium pollution caused by misoperation is avoided, and the experiment safety and the experiment result accuracy are improved.

Description

Technical Field

[0001] The invention relates to the technical field of bacteria experiment instruments, and in particular to a full-automatic bacteria experiment device and a use method thereof. Background Art

[0002] Bacteria are the basic materials for conducting research on microorganisms and life sciences. In the medical field, there is a complete set of bacterial strains for the preparation of diagnostic products, production of bacterial vaccines, research on microbial pathogenicity, antibacterial experiments of drugs, and microbial testing of drugs. Microorganisms have vitality and are prone to mutation and even death during the process of propagation. Therefore, bacterial propagation and preservation is an important basic work.

[0003] At present, laboratory bacteria-related experiments all require manual operation, which is cumbersome and time-consuming. Improper operation may lead to bacterial contamination, causing a series of safety hazards, which not only affects the experimental results, but also may harm the operators and cause infection with major dangerous pathogens. Improper operation of existing bacteria may cause the following hazards: 1. Laboratory safety risks: Improper operations in the laboratory may lead to bacterial contamination, which will not only affect the experimental results, but also cause cross-contamination within the laboratory, threatening the health of laboratory workers.

[0004] 2. Infection risk: Improper operation may cause workers to come into direct contact with harmful bacteria, increasing the risk of infection. For example, when taking seed tanks and fungus tanks, if you do not pay attention to hand hygiene or do not wear appropriate protective equipment, the risk of infection with bacteria and toxins will increase.

[0005] 3. Biological hazards: If pathogenic or toxic bacteria are involved in bacterial operations, incorrect operations may release these harmful substances into the environment, causing harm to the operator and others.

[0006] 4. Occupational health issues: Long-term exposure to improper bacterial operating environment may cause various occupational health problems, including but not limited to skin irritation, respiratory diseases, etc.

[0007] 5. Impact on experimental results: Bacterial contamination can lead to experimental failure because foreign bacteria will consume the nutrients in the culture medium, change the physical and chemical properties of the fermentation liquid, and affect the yield and quality of the product.

[0008] 6. Interference with the production process. In industrial production, bacterial contamination may lead to production interruptions, product quality problems, and even economic losses.

[0009] In summary, the hazards of improper handling of bacteria are mainly reflected in the potential threat to human health, the impact on experimental results, product quality problems and economic losses. In order to reduce these hazards, it is imperative to invent a device that can replace manual manipulation of bacteria. Summary of the invention

[0010] The purpose of the present invention is to solve the above existing technical problems and provide a fully automatic bacterial experiment equipment and its use method, which can reduce a series of safety hazards caused by operating bacteria. The specific technical solution is as follows: A fully automatic bacteria experiment device comprises a box body, a partition is arranged in the middle of the box body, and the box body is divided into two completely independent operation areas by the partition, one side is a bacteria freezing box, and the other side is a bacteria operation box; two symmetrical sealing door devices are arranged above and in front of the box body respectively, an ultraviolet lamp device is arranged inside the symmetrical sealing door device above the box body, a motor and a refrigeration and heating temperature control system device are arranged below the box body, an operation panel is arranged on the upper left side of the box body, and the operation panel is equipped with fully automatic bacteria operation software, and the operation panel is connected to a computer through a panel line.

[0011] Preferably, the test tube turntable a on one side of the bacterial freezing box transfers the test tube to the consumables area a through the test tube guide rail a, the culture dish loading area a transfers the culture dish to the consumables area a through the culture dish guide rail a, the multifunctional robotic arm a grabs the test tube or culture dish in the consumables area a, and transports it to the other side of the bacterial freezing box through the robotic arm running track a; the test tube or culture dish transported by the robotic arm running track a from the other side of the bacterial freezing box enters the freezing tube bottle loading area, the test tube or culture dish in the freezing tube bottle loading area enters the freezing bottle turntable, is transported to the laser printing area through the freezing bottle guide rail, and is transported to the low-temperature storage box through the freezing track disk.

[0012] Preferably, a waste storage box a is also provided inside the bacteria freezing box, and used consumables are collected in the waste storage box a.

[0013] Preferably, a rotating guide rail is provided inside the test tube turntable a, and the suspension, lyophilized powder and other substances inside the multiple test tubes placed in the test tube turntable a are shaken evenly by the rotating guide rail; a rotating guide rail is also provided inside the cryostat turntable, and the cryostat bottles are transported to the cryostat tube track disk by the rotating guide rail.

[0014] Preferably, a lifting platform a is provided directly below the cryogenic tube track tray, the ultraviolet-visible light zone a is provided on the left side of the equipment, and the light source is emitted from the left side; the lifting platform a distributes the cryogenic tubes to different layers in the low-temperature preservation box, and the ultraviolet-visible light zone a judges or measures the substance content according to the absorbance at the characteristic wavelength on the absorption spectrum.

[0015] Preferably, one side of the interior of the bacterial operation box is the same as the bacterial freezing box, including a test tube turntable b, a culture dish loading area b, a test tube track b, a culture dish track b, a waste storage box b, a consumables area b, a multifunctional robotic arm b, and a robotic arm running track b, and the other side is provided with a culture dish upper plate area, a test tube upper bottle area, a test tube track plate, a culture dish track plate, a test tube incubator, and a culture dish incubator; the test tubes in the test tube upper bottle area are transported to the test tube incubator via the test tube track plate, and the culture dishes in the culture dish upper plate area are transported to the culture dish incubator via the culture dish track plate.

[0016] Preferably, the test tube track tray is arranged in front of the test tube incubator, the culture dish track tray is arranged in front of the culture dish incubator, the lifting platform b is arranged directly below the test tube track tray and the culture dish track tray, the laser printing area is arranged directly above the culture dish track 2 and the test tube track 2, the ultraviolet visible light area b is arranged on the left side of the equipment, the light source is emitted from the left side, and the optical imaging system is arranged directly above the culture dish track b; the lifting platform b distributes the cryopreserved tubes to different layers in the incubator, the laser printing area collects information on the strains after the cryopreservation is completed, the ultraviolet visible light area b judges or measures the substance content according to the absorbance at the characteristic wavelength on the absorption spectrum, and the optical imaging system is connected to a computer via a data cable for image analysis.

[0017] Preferably, the multifunctional robotic arm a and the multifunctional robotic arm b are each provided with 10 arms, namely, a flat plate opening and closing gripper, a sampling needle, a magnetic bead clamping tweezers, an inoculation loop, a screw-cap bottle knob, a homogenizer gripper, a test tube opening and closing gripper, a grinding wheel, a sealing device, and a high-pressure nozzle.

[0018] A method for using a fully automatic bacterial experiment device, using the fully automatic bacterial experiment device to freeze liquid bacterial strain magnetic beads, comprising the following steps: Step 1: First, turn on the machine and set the strain freezing program and sample information on the operation panel, and then add the consumables and reagents used; Step 2: Place the liquid bacterial suspension test tube on the test tube turntable a, and the test tube turntable a rotates to transport the liquid bacterial suspension test tube to the test tube guide a, and then transport it to the consumable area a through the test tube guide a; at the same time, analyze the sample concentration through the ultraviolet visible light area and calculate the frozen bacterial strain sampling amount according to the set concentration; Step 3: Open the liquid bacterial suspension test tube through the test tube opening and closing gripper on the multifunctional robotic arm a, blow the bacterial solution in the liquid bacterial suspension test tube first to ensure that the bacterial solution is mixed, and then absorb the required number of samples, and the test tube opening and closing gripper on the multifunctional robotic arm a covers the test tube, and transports the used sample to the waste storage box a through the test tube guide a, and at the same time, the multifunctional robotic arm a transports the liquid bacterial suspension test tube on the robotic arm running track a to the top of the cryopreservation bottle turntable; Step 4: The screw-mouth bottle knob on the multifunctional robotic arm a opens the cryotube cover, the sampling needle on the multifunctional robotic arm a adds the sample into the cryotube, the screw-mouth bottle knob on the multifunctional robotic arm a closes the cryotube cover, and the homogenizing gripper on the multifunctional robotic arm a homogenizes the cryotube; Step 5: After homogenization, the cryotube is placed back on the cryotube turntable. First, the screw-mouth bottle knob on the multifunctional robotic arm a opens the cryotube lid. Then, the sampling needle on the multifunctional robotic arm a sucks out all the liquid in the cryotube. Then, the screw-mouth bottle knob on the multifunctional robotic arm a closes the cryotube lid. Finally, the automatic sealer on the multifunctional robotic arm a seals the cryotube. Step 6: The cryostat carousel transports the frozen samples to the cryostat guide rail, and the sample-related information is marked through the laser printing area. The cryostat guide rail transports the samples to the cryostat track disk, and the cryostat track disk transports the samples to the low-temperature storage box by lifting. The program is now complete.

[0019] Compared with the closest prior art, the technical solution provided by the present invention has the following beneficial effects: The fully automatic bacterial experiment equipment of the present invention can replace manual bacterial manipulation. The fully automated operation steps are simple and efficient, and the full automation of bacteria-related experiments is realized, thereby avoiding the problem of bacterial contamination caused by improper operation, and improving the safety of the experiment and the accuracy of the experimental results. It can save a lot of time and labor costs in basic scientific research, clinical diagnosis, disease prevention and control, and drug development. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] Figure 1 This is an overall schematic diagram of a fully automatic bacterial experiment device of the present invention; Figure 2 This is a schematic diagram of the interior of the bacteria freezing box of the present invention; Figure 3 This is a schematic diagram of the interior of the bacterial operation box of the present invention; Figure 4 This is a schematic diagram of the multifunctional mechanical arm of the present invention; Figure 5 It is a schematic diagram of the lifting platform of the present invention; Among them: 1. Bacteria freezing box, 2. Bacteria operation box, 3. Sealed door device, 4. Ultraviolet lamp device, 5. Operation panel, refrigeration and heating temperature control system device 6, 1-1. Test tube turntable a, 1-2. Culture dish loading area a, 1-3. Test tube guide a, 1-4. Culture dish guide a, 1-5. Waste storage box a, 1-6. Consumables area a, 1-7. Multifunctional mechanical arm a, 1-8. Mechanical arm running track a, 1-9. Cryogenic tube loading area, 1-10. Cryogenic bottle turntable, 1-11. Cryogenic bottle guide, 1-12. Laser printing area, 1-13. Cryogenic track disk, 1-14. Low temperature storage box, 1-15. Lifting platform a, 1-16. Ultraviolet visible light area a, 2- 1. Test tube turntable b, 2-2. Culture dish loading area b, 2-3. Test tube guide b, 2-4. Culture dish guide b, 2-5. Waste storage box b, 2-6. Consumables area b, 2-7. Multifunctional robotic arm b, 2-8. Robotic arm running track b, 2-9. Culture dish upper plate area, 2-10. Test tube bottle area, 2-11. Test tube track plate, 2-12. Culture dish track plate, 2-13. Test tube incubator, 2-14. Culture dish incubator, 2-15. Track plate track rack, 2-16. Lifting platform b, 2-17. Laser printing area, 2-18. Ultraviolet visible light area b, 2-19. Optical imaging system, 2-20 Culture dish track 2, 2-21 Test tube track 2. DETAILED DESCRIPTION

[0021] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0022] See also Figure 1-5 .

[0023] Example 1 A fully automatic bacteria experiment device comprises a box body, a partition is arranged in the middle of the box body, and the box body is divided into two completely independent operation areas by the partition, one side is a bacteria freezing box 1, and the other side is a bacteria operation box 2; two symmetrical sealing door devices 3 are arranged above and in front of the box body respectively, an ultraviolet lamp device 4 is arranged inside the symmetrical sealing door device 3 above the box body, a motor and a refrigeration and heating temperature control system device 6 are arranged below the box body, an operation panel 5 is arranged on the upper left side of the box body, and the operation panel 5 is equipped with fully automatic bacteria operation software, and the operation panel 5 is connected to a computer through a panel line.

[0024] Furthermore, the test tube turntable a1-1 on one side of the bacterial freezing box 1 transfers the test tube to the consumables area a1-6 through the test tube guide a1-3, and the culture dish loading area a1-2 transfers the culture dish to the consumables area a1-6 through the culture dish guide a1-4. A waste storage box a1-5 is also provided inside the bacterial freezing box 1, and used consumables are collected in the waste storage box a1-5; the multifunctional robotic arm a1-7 grabs the test tube or culture dish in the consumables area a1-6 , and transported to the other side of the bacterial freezing box 1 through the robot arm running track a1-8; the test tubes or culture dishes transported by the robot arm running track a1-8 from the other side of the bacterial freezing box 1 enter the freezing tube bottle area 1-9, and the test tubes or culture dishes in the freezing tube bottle area 1-9 enter the freezing bottle turntable 1-10, are transported to the laser printing area 1-12 through the freezing bottle guide rail 1-11, and are transported to the low-temperature preservation box 1-14 through the freezing track disk 1-13.

[0025] Furthermore, a rotating guide rail is provided inside the test tube turntable a1-1, and the suspension, lyophilized powder and other substances inside the multiple test tubes placed in the test tube turntable a1-1 are shaken evenly by the rotating guide rail; a rotating guide rail is also provided inside the cryostat bottle turntable 1-10, and the cryostat bottles are transported to the cryostat tube track disk 1-13 by the rotating guide rail.

[0026] Furthermore, the lifting platform a1-15 distributes the cryotubes to different layers in the low-temperature preservation box, and the ultraviolet visible light area a1-16 judges or measures the content of the substance according to the absorbance at the characteristic wavelength on the absorption spectrum.

[0027] Furthermore, one side of the interior of the bacterial operation box 2 is the same as the bacterial freezing box 1, including a test tube turntable b2-1, a culture dish loading area b2-2, a test tube track b2-3, a culture dish track b2-4, a waste storage box b2-5, a consumables area b2-6, a multifunctional robotic arm b2-7, and a robotic arm running track b2-8, and the other side is provided with a culture dish upper plate area 2-9, a test tube upper bottle area 2-10, a test tube track plate 2-11, a culture dish track plate 2-12, a test tube incubator 2-13, and a culture dish incubator 2-14; the test tubes in the test tube upper bottle area 2-10 are transported to the test tube incubator 2-13 via the test tube track plate 2-11, and the culture dishes in the culture dish upper plate area 2-9 are transported to the culture dish incubator 2-14 via the culture dish track plate 2-12.

[0028] Furthermore, the lifting platform b2-16 distributes the cryopreservation tubes to different layers in the incubator, the laser printing area 2-17 collects information on the strains after they are frozen, the ultraviolet visible light area b2-18 determines or measures the substance content based on the absorbance at the characteristic wavelength on the absorption spectrum, and the optical imaging system 2-19 is connected to a computer via a data cable for image analysis.

[0029] Furthermore, the multifunctional robotic arm a1-7 and the multifunctional robotic arm b2-7 are both provided with 10 arms, namely, a dish cover opening and closing gripper, a sampling needle, a magnetic bead gripping tweezers, an inoculation loop, a screw-cap bottle knob, a homogenizing gripper, a test tube opening and closing gripper, a grinding wheel, a sealing device, and a high-pressure nozzle.

[0030] Furthermore, the robot arm running track runs through the entire box, transporting from one side of the box to the other side.

[0031] A method for using a fully automatic bacterial experiment device, using the fully automatic bacterial experiment device to freeze liquid bacterial strain magnetic beads, comprising the following steps: Step 1: Turn on the machine, set the strain freezing program and sample information on the operation panel 5, and add the consumables and reagents used; Step 2: Place the liquid bacterial suspension test tube on the test tube turntable a1-1, and the test tube turntable a1-1 rotates to transport the liquid bacterial suspension test tube to the test tube guide a1-3, and then transport it to the consumables area a1-6 through the test tube guide a1-3; at the same time, analyze the sample concentration through the ultraviolet visible light area 1-16 and calculate the frozen bacterial strain sampling amount according to the set concentration; Step 3: Open the liquid bacterial suspension test tube through the test tube opening and closing gripper on the multifunctional robotic arm a1-7, blow the bacterial solution in the liquid bacterial suspension test tube with the sampling needle on the multifunctional robotic arm a1-7 first to ensure that the bacterial solution is mixed and then absorb the required number of samples, cover the test tube with the test tube opening and closing gripper on the multifunctional robotic arm a1-7, and transport the used sample to the waste storage box a1-5 through the test tube guide a1-3, and at the same time, the multifunctional robotic arm a1-7 transports the liquid bacterial suspension test tube to the top of the cryopreservation bottle turntable 1-10 on the robotic arm running track a1-8; Step 4: The multifunctional robotic arm a1-7 uses the screw-mouth bottle knob to open the cryotube cover, the multifunctional robotic arm a1-7 uses the sampling needle to add the sample into the cryotube, the multifunctional robotic arm a1-7 uses the screw-mouth bottle knob to close the cryotube cover, and the multifunctional robotic arm a1-7 uses the homogenizing gripper to homogenize the cryotube. Step 5: After homogenization, put the cryotube back on the cryotube turntable 1-10. First, the screw-mouth bottle knob on the multifunctional robotic arm a1-7 opens the cryotube cover. Then, the sampling needle on the multifunctional robotic arm a1-7 sucks out all the liquid in the cryotube. Then, the screw-mouth bottle knob on the multifunctional robotic arm a1-7 closes the cryotube cover. Finally, the automatic sealer on the multifunctional robotic arm a1-7 seals the cryotube. Step 6: The cryostat turntable 1-10 transports the frozen samples to the cryostat guide 1-11, and the sample-related information is marked through the laser printing area 1-12. The cryostat guide 1-11 transports the samples to the cryostat track disk 1-13, and the cryostat track disk 1-13 transports the samples to the low-temperature preservation box 1-14 through the lifting platform 1-15. The program is now completed.

[0032] Example 2 A method for using a fully automatic bacterial experiment device, using the fully automatic bacterial experiment device to perform a solid bacteria purification operation process, comprising the following steps: Step 1: Turn on the machine, set the solid bacteria purification program and sample information on the computer or operation panel, and add the consumables and reagents used; Step 2: Place the sample to be purified on the culture dish loading area b2-2, transfer the sample to the culture dish track b2-4, quickly scan and image through the optical imaging system 2-19, connect to the computer through a data cable to perform image analysis, and find the target colony; Step 3: The multifunctional robot arm b2-7 opens the lid with the plate opening and closing gripper, collects the target colony with the inoculation loop, and closes the lid with the plate opening and closing gripper on the multifunctional robot arm b2-7. The culture dish track b2-4 transports the used sample to the waste storage box b2-5. At the same time, the multifunctional robot arm b2-7 runs on the robot arm running track b2-8 to the culture dish track. The culture dish track continues to transport the culture medium with the sample information printed on it through the laser printing area 2-17. Step 4: The flat dish cover opening and closing gripper on the multifunctional robotic arm b2-7 opens the culture medium cover with the sample information printed on it, the inoculation loop on the multifunctional robotic arm b2-7 that has collected samples divides the culture medium into sections and marks the lines, the flat dish cover opening and closing gripper on the multifunctional robotic arm b2-7 closes the culture medium cover, the multifunctional robotic arm b2-7 transports the used inoculation loop to the waste storage box b2-5, the culture dish track transports the culture medium to the culture dish track tray b2-12, the culture dish track tray b2-12 transports the culture medium to the culture dish incubator b2-14 via the lifting platform b2-16, and the program ends here.

[0033] Example 3 A method for using a fully automatic bacterial experiment device, using the fully automatic bacterial experiment device to perform a microbial limit inspection operation process, including the following steps: Step 1: Turn on the machine, set the microbial limit test program and sample information on the computer or operation panel, and add the consumables and reagents used; Step 2: Place the sample on the test tube turntable b2-1. According to the set sample dilution concentration, the test tube opener and closeer on the multifunctional robotic arm b2-7 opens the test tube. The sampling needle on the multifunctional robotic arm b2-7 goes to the consumable area b2-6 to absorb a certain amount of diluent into the test tube. The test tube opener and closeer on the multifunctional robotic arm 2-7 covers the test tube lid. The homogenizing gripper on the multifunctional robotic arm b2-7 homogenizes the test tube (the homogenization method can be set, including but not limited to shaking and inversion). After homogenization, put it back on the test tube turntable b2-1. Step 3: The multifunctional robotic arm b2-7 uses a test tube opener to open the second test tube, and the multifunctional robotic arm b2-7 uses a sampling needle to go to the consumable area b2-6 to absorb a certain amount of diluent into the second test tube. The multifunctional robotic arm b2-7 uses a test tube opener to open the first test tube, and the multifunctional robotic arm b2-7 uses a sampling needle to go to the first test tube to absorb a certain amount of sample into the second test tube. Step 4: The test tube opener and closeer on the multifunctional robotic arm b 2-7 covers the first and second test tubes, and the homogenizing gripper on the multifunctional robotic arm b 2-7 homogenizes the second test tube (the homogenization method can be set, including but not limited to shaking and inversion), and puts it back on the test tube turntable b 2-1 after homogenization (if gradient dilution needs to be continued, proceed in the same way as above); Step 5: The test tube turntable b2-1 transports the sample to the test tube track b2-3, the test tube opener on the multifunctional robotic arm b2-7 opens the first test tube, the sampling needle on the multifunctional robotic arm b2-7 absorbs a certain amount of liquid in the first test tube, and the multifunctional robotic arm b2-7 runs on the robotic arm running track b2-8 to the culture dish track; Step 6: The culture dish track will continue to transport the culture medium with the sample information printed on it through the laser printing area 2-17. The multifunctional robot arm b2-7 will open the culture medium cover with the sample information printed on it. The multifunctional robot arm b2-7 will use the sampling needle to suck the liquid in the first test tube into the culture medium with the sample information printed on it. The multifunctional robot arm will use the sampling needle to transport it to the waste storage box b2-5. Step 7: The plate cover opening and closing gripper on the multifunctional robotic arm b2-7 covers the culture medium cover with the printed sample information, and the homogenizing gripper on the multifunctional robotic arm b2-7 homogenizes the culture medium injected into the first test tube sample (the homogenization method can be set, including but not limited to shaking and inversion), and puts it back on the culture plate track after homogenization, and the test tube opener and closer on the multifunctional robotic arm b2-7 opens the second test tube, and the sampling needle on the multifunctional robotic arm b2-7 absorbs a certain amount of liquid in the second test tube, and at the same time, the multifunctional robotic arm b2-7 runs on the robotic arm running track b2-8 to the culture plate track; Step 8: The culture dish track will continue to transport the culture medium with the sample information printed on it through the laser printing area 2-17, the plate cover opening and closing gripper on the multifunctional robotic arm b2-7 will open the cover of the culture medium with the sample information printed on it, the sampling needle on the multifunctional robotic arm b2-7 will suck the liquid in the second test tube into the culture medium with the sample information printed on it, the multifunctional robotic arm b2-7 will use the sampling needle to transport it to the waste storage box b2-5, the plate cover opening and closing gripper on the multifunctional robotic arm b2-7 will cover the cover of the culture medium with the sample information printed on it, and the homogenizing gripper on the multifunctional robotic arm b2-7 will homogenize the culture medium injected into the second test tube sample (the homogenization method can be set, including but not limited to shaking and inversion); Step 9: The test tube guide b 2-3 transports the used test tubes to the waste storage box a 1-5, and puts them back on the culture dish track after homogenization. The culture dish track transports the culture medium to the culture dish track tray 2-12, and the culture dish track tray 2-12 transports the culture medium to the culture dish incubator 2-14 through the lifting platform a 1-15. At this point, the program is completed.

[0034] Example 4 A method for using a fully automatic bacterial experimental device, using the fully automatic bacterial experimental device to perform a drug minimum inhibitory concentration MIC determination process, comprising the following steps: Step 1: Turn on the machine, set the drug minimum inhibitory concentration MIC determination program and sample information on the computer or operation panel, and add the consumables and reagents (drugs) used; Step 2: Place the bacterial suspension on the test tube turntable b2-1, and the test tube turntable will rotate to transport the bacterial suspension to the test tube guide a1. The sample concentration is analyzed by the ultraviolet visible light area b2-18 and the bacterial solution sampling volume is calculated according to the set concentration. The test tube opener and closeer on the multifunctional robotic arm a1-7 opens the test tubes 1, 2, 3 (1, 2, 3 are three parallel groups of the first gradient) 4, 5, 6 (4, 5, 6 are three parallel groups of the second gradient) 7, 8, 9 (7, 8, 9 are three parallel groups of the third gradient) on the test tube track 2. The sampling needle on the multifunctional robotic arm a1-7 draws a certain amount of liquid culture medium into the test tubes 1, 2, 3, 4, 5, 6, 7, 8, 9. The sampling needle on the multifunctional robotic arm a1-7 first blows the bacterial solution to ensure that the bacterial solution is mixed, and then draws the same amount of bacterial solution into the test tubes 1, 2, 3, 4, 5, 6, 7, 8, 9. Step 3: The multifunctional robotic arm a 1-7 replaces the sampling needle to absorb the set concentration of the drug and adds it to the first gradient test tubes 1, 2, and 3 respectively. The multifunctional robotic arm a 1-7 replaces the sampling needle to absorb the set concentration of the drug and adds it to the second gradient test tubes 4, 5, and 6 respectively. The multifunctional robotic arm a 1-7 replaces the sampling needle to absorb the set concentration of the drug and adds it to the third gradient test tubes 7, 8, and 9 respectively. Step 4: The test tube opener and closer on the multifunctional robotic arm b 2-7 covers the lids of the test tubes 1, 2, 3, 4, 5, 6, 7, 8, and 9. The homogenizing gripper on the multifunctional robotic arm 2-7 homogenizes the test tubes 1, 2, 3, 4, 5, 6, 7, 8, and 9 (the homogenization method can be set, including but not limited to shaking and inversion). After homogenization, they are put back on the test tube track 2. The automatic sealer on the multifunctional robotic arm b 2-7 seals the test tubes 1, 2, 3, 4, 5, 6, 7, 8, and 9 (the sealing method includes but is not limited to sealing film and paraffin). The sample related information is marked by the laser printing area 2-17. The test tube track 2 transports the test tubes 1, 2, 3, 4, 5, 6, 7, 8, and 9 to the test tube track plate b 2-11. The test tube track plate 2-11 transports the culture medium to the test tube incubator 2-13 through the lifting platform b 2-16. At this point, the program is completed.

[0035] Example 5 A method for using a fully automatic bacterial experiment device, using the fully automatic bacterial experiment device for cleaning and disinfection (taking the disinfectant method as an example) operation process, including the following steps: Step 1: Set up the cleaning and disinfection program (including but not limited to disinfectant, dry heat sterilization, ultraviolet, fumigation) and sample information on the computer or operation panel, and then add the consumables and reagents used; Step 2: A conduit is provided at the rear end of the high-pressure nozzle on the multifunctional robotic arm b 2-7 to directly connect to the disinfectant bottle, and the disinfectant is transported to the high-pressure nozzle by pressurization. The high-pressure nozzle on the multifunctional robotic arm b 2-7 runs on the robotic arm track b 2-8 to spray the disinfectant to every place in the equipment. At this point, the program is completed.

[0036] The above embodiments are only used to illustrate the technical solutions of the present invention rather than to limit the same. Although the present invention has been described in detail with reference to the above embodiments, a person skilled in the art can still modify or make equivalent substitutions to the specific implementations of the present invention. Any modifications or equivalent substitutions that do not depart from the spirit and scope of the present invention are within the scope of protection of the claims of the present invention to be approved.

Claims

1. A fully automatic bacterial experiment equipment, comprising a box, characterized in that: A partition is arranged in the middle of the box body, and the box body is divided into two completely independent operation areas by the partition, one side is a bacteria freezing box (1), and the other side is a bacteria operation box (2); two symmetrical sealing door devices (3) are arranged above and in front of the box body respectively, and an ultraviolet lamp device (4) is arranged inside the symmetrical sealing door device (3) above the box body, and a motor and a refrigeration and heating temperature control system device are arranged below the box body. An operation panel (5) is arranged at the upper left of the box body, and the operation panel (5) is equipped with fully automatic bacteria operation software, and the operation panel (5) is connected to a computer through a panel line.

2. A fully automatic bacterial experiment equipment according to claim 1, characterized in that: The test tube turntable a (1-1) on one side of the bacterial freezing box (1) transfers the test tube to the consumables area a (1-6) via the test tube guide a (1-3), the culture dish loading area a (1-2) transfers the culture dish to the consumables area a (1-6) via the culture dish guide a (1-4), and the multifunctional robotic arm a (1-7) grabs the test tube or culture dish in the consumables area a (1-6) and transports it to the bacterial freezing box via the robotic arm running track a (1-8). (1) The other side inside; the test tube or culture dish transported by the robot arm running track a (1-8) on the other side inside the bacterial freezing box (1) enters the freezing tube bottle loading area (1-9), the test tube or culture dish in the freezing tube bottle loading area (1-9) enters the freezing bottle turntable (1-10), is transported to the laser printing area (1-12) through the freezing bottle guide rail (1-11), and is transported to the low temperature preservation box (1-14) through the freezing track disk (1-13).

3. A fully automatic bacterial experiment equipment according to claim 2, characterized in that: A waste storage box a (1-5) is also provided inside the bacterial freezing box (1), and used consumables are collected in the waste storage box a (1-5).

4. A fully automatic bacterial experiment equipment according to claim 2, characterized in that: The test tube turntable a (1-1) is provided with a rotating guide rail, and the suspension, lyophilized powder and other substances in the multiple test tubes placed in the test tube turntable a (1-1) are shaken evenly by the rotating guide rail; the cryostat bottle turntable (1-10) is also provided with a rotating guide rail, and the cryostat bottles are transported to the cryostat tube track plate (1-13) by the rotating guide rail.

5. A fully automatic bacterial experiment equipment according to claim 2, characterized in that: A lifting platform a (1-15) is arranged directly below the cryotube track plate (1-13); the ultraviolet-visible light zone a (1-16) is arranged on the left side of the device, and the light source is emitted from the left side; the lifting platform a (1-15) distributes the cryotubes to different layers in the low-temperature preservation box, and the ultraviolet-visible light zone a (1-16) judges or measures the content of the substance according to the absorbance at the characteristic wavelength on the absorption spectrum.

6. A fully automatic bacterial experiment equipment according to claim 1, characterized in that: One side of the interior of the bacterial operation box (2) is the same as the bacterial freezing box (1), comprising a test tube turntable b (2-1), a culture dish loading area b (2-2), a test tube track b (2-3), a culture dish track b (2-4), a waste storage box b (2-5), a consumables area b (2-6), a multifunctional mechanical arm b (2-7), and a mechanical arm running track b (2-8); the other side is provided with a culture dish upper plate area (2-9), a test tube upper bottle area (2-10), a test tube track plate (2-11), a culture dish track plate (2-12), a test tube incubator (2-13), and a culture dish incubator (2-14); the test tubes in the test tube upper bottle area (2-10) are transported to the test tube incubator (2-13) via the test tube track plate (2-11), and the culture dishes in the culture dish upper plate area (2-9) are transported to the culture dish incubator (2-14) via the culture dish track plate (2-12).

7. A fully automatic bacterial experiment equipment according to claim 6, characterized in that: The test tube track plate (2-11) is arranged in front of the test tube incubator (2-13), the culture dish track plate (2-12) is arranged in front of the culture dish incubator (2-14), the lifting platform b (2-16) is arranged directly below the test tube track plate (2-11) and the culture dish track plate (2-12), the laser printing area (2-17) is arranged directly above the culture dish track 2 (2-20) and the test tube track 2 (2-21), the ultraviolet visible light area b (2-18) is arranged on the left side of the device, and the light The source is emitted from the left side, and the optical imaging system (2-19) is set directly above the culture dish track b (2-4); the lifting platform b (2-16) distributes the cryopreservation tubes to different layers in the incubator, the laser printing area (2-17) collects information on the strains after they are frozen, and the ultraviolet visible light area b (2-18) judges or measures the substance content according to the absorbance at the characteristic wavelength on the absorption spectrum. The optical imaging system (2-19) is connected to a computer via a data cable for image analysis.

8. A fully automatic bacterial experiment equipment according to claim 7, characterized in that: The multifunctional mechanical arm a (1-7) and the multifunctional mechanical arm b (2-7) are both provided with 10 arms, which are respectively a plate opening and closing gripper, a sampling needle, a magnetic bead gripping tweezers, an inoculation loop, a screw-mouth bottle knob, a homogenizing gripper, a test tube opening and closing gripper, a grinding wheel, a sealing device, and a high-pressure nozzle.

9. A method for using a fully automatic bacterial experiment device as described in any one of claims 1 to 8, characterized in that: Using the fully automatic bacterial experimental equipment to freeze liquid bacterial strains with magnetic beads includes the following steps: Step 1: First, turn on the machine and set the strain freezing program and sample information on the operation panel (5), and then add the consumables and reagents used; Step 2: Place the liquid bacterial suspension test tube on the test tube turntable a (1-1), and the test tube turntable a (1-1) rotates to transport the liquid bacterial suspension test tube to the test tube guide a (1-3), and then transports it to the consumables area a (1-6) through the test tube guide a (1-3); at the same time, analyze the sample concentration through the ultraviolet visible light area (1-16) and calculate the frozen bacterial strain sampling amount according to the set concentration; Step 3: Open the liquid bacterial suspension test tube by using the test tube opening and closing gripper on the multifunctional robotic arm a (1-7), blow the bacterial solution in the liquid bacterial suspension test tube with the sampling needle on the multifunctional robotic arm a (1-7) to ensure that the bacterial solution is mixed, and then absorb the required number of samples, and cover the test tube with the test tube opening and closing gripper on the multifunctional robotic arm a (1-7), and transport the used sample to the waste storage box a (1-5) through the test tube guide a (1-3), and at the same time, the multifunctional robotic arm a (1-7) transports the liquid bacterial suspension test tube on the robotic arm running track a (1-8) to the top of the cryopreservation bottle turntable (1-10); Step 4: The multifunctional robotic arm a (1-7) uses the screw-cap bottle knob to open the cryotube cover, the multifunctional robotic arm a (1-7) uses the sampling needle to add the sample into the cryotube, the multifunctional robotic arm a (1-7) uses the screw-cap bottle knob to close the cryotube cover, and the multifunctional robotic arm a (1-7) uses the homogenizing gripper to homogenize the cryotube. Step 5: After homogenization, place the cryotube back on the cryotube turntable (1-10). First, the multifunctional robotic arm a (1-7) opens the cryotube lid by turning the screw-cap bottle knob. Then, the multifunctional robotic arm a (1-7) uses the sampling needle to suck out all the liquid in the cryotube. Then, the multifunctional robotic arm a (1-7) uses the screw-cap bottle knob to close the cryotube lid. Finally, the multifunctional robotic arm a (1-7) uses the automatic sealer to seal the cryotube. Step 6: The cryostat carousel (1-10) transports the frozen samples to the cryostat guide (1-11), and the sample-related information is marked through the laser printing area (1-12). The cryostat guide (1-11) transports the samples to the cryostat track disk (1-13), and the cryostat track disk (1-13) transports the samples to the low-temperature storage box (1-14) through the lifting platform (1-15). The program is now complete.

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