Continuous flow magnetic field biological fermentation and catalytic synthesis device and method

Abstract

The invention discloses a continuous flow magnetic field biological fermentation and catalytic synthesis device and method. The device comprises a heat preservation container, a magnetic field assembly and a conduction cavity. The magnetic field assembly is fixedly carried in the heat preservation container. The heat preservation container is of a closed structure with an inlet and an outlet, and a constant-temperature medium can be introduced and circularly flows. The conducting cavity penetrates through the magnetic field assembly, and all the sample tubes can pass through the conducting cavity; the fermentation liquid or the reaction medium continuously flows in the sample tube and is subjected to the action of the magnetic field; the included angle between the magnetic line direction of the magnetic field and the flowing direction of the fermentation liquid or the reaction medium is 0-90 degrees. The continuous flow magnetic field fermentation and catalysis device provided by the invention can realize magnetic field continuous catalysis treatment of fermentation liquor or a reaction medium in an external circulation state, and the adopted magnetic field assembly has a heat dissipation structure, so that heat dissipation and temperature control on the magnetic field assembly by a constant-temperature medium are facilitated, and the working stability and controllability of the device are improved; meanwhile, the fermentation temperature or the reaction temperature is not interfered by the temperature rise of the magnetic field assembly.

Description

Technical Field

[0001] The present invention particularly relates to a continuous flow magnetic field biological fermentation and catalytic synthesis device and method, belonging to the technical field of biological fermentation and catalytic synthesis. Background Art

[0002] In the context of limited resources, low-carbon and sustainable development strategies must be adopted to reduce the environmental pressure caused by huge energy demand. Liquid fermentation culture and chemical synthesis technology provide an effective way to obtain high-quality and high-yield products, such as bioactive substances, fuels, foods, fine chemicals and drugs. In recent years, with the in-depth study of the biological effects of magnetic fields, magnetic field-assisted microbial fermentation, cell culture and chemical catalytic reactions have become popular. Related studies have found that magnetic fields can regulate the process of microbial fermentation, cell metabolism and chemical catalytic reactions through macroscopic and microscopic pathways. The main principles include three aspects: 1) Intracellular substances such as proteins, nucleic acids, enzymes and free radicals are affected by external magnetic fields, namely Lorentz force effect, eddy current effect, induced current effect and additional magnetic moment, etc., to induce metabolic activities and changes in physical and chemical properties; 2) The magnetic field acts on the fermentation, culture and reaction matrix, inducing water molecule polarization and ion migration, thereby promoting the release of internal dissolved oxygen or carbon monoxide; 3) The magnetic field acts on the biomembrane system of microorganisms, changing the permeability and fluidity of the membrane, thereby regulating the intracellular ion transmembrane transport, signal transduction and electron transfer processes. Based on the above research, appropriate magnetic field treatment is beneficial to promoting biological conversion rate and catalytic reaction rate, and at the same time has the significant advantages of high efficiency, energy saving, low carbon and environmental protection.

[0003] Traditional magnetic field-assisted fermentation culture and catalytic reaction devices mostly adopt intermittent static treatment, and permanent magnets or conductive coils are set outside the fermenter or reactor so that the magnetic field covers the entire fermentation or reaction system, which undoubtedly increases the load of the fermenter or reactor. At the same time, the controllability of the magnetic field intensity and frequency is insufficient, and the working parameter range is limited, that is, the magnetic field energy density caused by low frequency is small, which leads to poor effects of biological fermentation culture and synthetic catalytic reactions. In recent years, continuous flow magnetic field fermentation and catalytic devices have attracted widespread attention. For example, in the prior art, the magnetic field assembly is vertically or radially installed on both sides of a straight tube, a spiral tube or a U-shaped tube, which can realize continuous magnetic field treatment under the external circulation state of the material, thereby improving the fermentation, culture or catalytic efficiency. However, the traditional magnetic field assembly cannot ensure that the sample is subjected to the same magnetic flux when in use, that is, due to the uneven distribution of magnetic lines of force, the application effect will not be repeatable, and thus the effect of a stable magnetic field environment on biological fermentation or catalytic synthesis reactions cannot be effectively regulated. Therefore, it is necessary to limit the distance between the material liquid in the sample tube and the magnetic field assembly, the relationship between the flow direction and the direction of the magnetic lines of force, avoid the uneven magnetic field environment and ensure that the sample is subjected to a stable magnetic flux. In addition, if the excitation heat cannot be removed by effective methods, it will inevitably be transferred to the fermentation liquid or reaction medium, causing the system to heat up or produce large temperature fluctuations, which will also interfere with the fermentation culture or catalytic reaction efficiency, further reduce repeatability, and fail to generate a high-energy density magnetic field, thus limiting the large-scale application of this method. Summary of the invention

[0004] The main purpose of the present invention is to provide a continuous flow magnetic field biological fermentation and catalytic synthesis device and method, thereby overcoming the deficiencies in the prior art.

[0005] In order to achieve the above-mentioned invention object, the technical solution adopted by the present invention includes: A first aspect of an embodiment of the present invention provides a continuous flow magnetic field biological fermentation and catalytic synthesis device, comprising: A constant temperature maintenance unit, comprising a sealed heat preservation container and a constant temperature medium circulation supply mechanism, wherein the heat preservation container has a sealed constant temperature cavity and x conductive cavities penetrating the container, wherein the conductive cavity is surrounded by the constant temperature cavity and is heat-conductingly matched with the constant temperature cavity, and the constant temperature medium circulation supply mechanism is connected with the constant temperature cavity to form a constant temperature medium circuit for continuous and circulating constant temperature medium; The reaction unit comprises a sample container and y sample tubes, wherein the sample container is used at least to contain a feed liquid and provide a required environment for fermentation culture or catalytic synthesis reaction of the feed liquid, each of the sample tubes is connected to the sample container to form a feed liquid loop for continuous flow of the feed liquid, and at least a part of each of the sample tubes is disposed in a conduction cavity and is thermally coordinated with the conduction cavity; A magnetic field generating unit comprises y magnetic field components, wherein the y magnetic field components are arranged in the constant temperature cavity, each of the magnetic field components is correspondingly distributed around one of the conductive cavities, and the magnetic field components are at least used to generate a magnetic field passing through the conductive cavity, and the angle between the direction of the magnetic field lines of the magnetic field generated by the magnetic field components and the axial direction of the sample tube passing through the magnetic field components is 0°~90°; the magnetic field can change the physical and chemical parameters of the medium or liquid in the sample tube, x≥y≥1, and x and y are positive integers.

[0006] In a more specific implementation, the constant temperature maintaining unit further includes y heat dissipation structures, the y heat dissipation structures are arranged in the constant temperature cavity, and each of the heat dissipation structures is arranged around a conductive cavity and thermally cooperates with the conductive cavity.

[0007] Furthermore, the constant temperature cavity includes a first constant temperature cavity and y second constant temperature cavities, the y second constant temperature cavities are surrounded by and connected to the first constant temperature cavity, each of the second constant temperature cavities is arranged around a conductive cavity and cooperates with the conductive cavity for thermal conductivity, and the second constant temperature cavity and the constant temperature medium located in the second constant temperature cavity serve as the heat dissipation structure.

[0008] Furthermore, the thermal insulation container includes a first shell and x second shells, the first shell and the x second shells are fixedly combined, the first shell and the x second shells enclose the constant temperature cavity, and each second shell encloses the conductive cavity, wherein the first shell is formed of a metal material or a non-metal material, and the second shell is formed of a non-metal material, specifically, the metal material includes aluminum, iron or stainless steel, and the non-metal material includes tetrafluoroethylene, PFA, polyethylene or epoxy resin, etc.

[0009] Furthermore, the magnetic field component is disposed around the periphery of the heat dissipation structure, or the magnetic field component is disposed between the heat dissipation structure and the conductive cavity.

[0010] Furthermore, the magnetic field component includes a conductive coil, which is wound around the periphery of the conductive cavity, and the conductive coil generates the magnetic field by connecting to a power supply; or, the magnetic field component includes at least one permanent magnet.

[0011] Furthermore, the wire diameter of the conductive coil is 0.01 mm to 5 mm.

[0012] Furthermore, the number of turns of each of the conductive coils is between 10 and 30,000 turns.

[0013] Furthermore, the magnetic field is an alternating magnetic field or a static magnetic field. The alternating magnetic field is generated by passing alternating current into the conductive coil, the static magnetic field is generated by passing direct current into the conductive coil, or the static magnetic field is generated by the permanent magnet.

[0014] Furthermore, the conductive coil is electrically connected to a power supply, the current provided by the power supply is 0.1-16 A, and the power supply is a DC power supply or an AC power supply.

[0015] Furthermore, the y magnetic field components are arranged in parallel, and the ratio of the outer edge circumference of the magnetic field component to the center distance between two adjacent magnetic field components is (1:0.15) to (1:20), for example, 1:0.15, 1:0.3, 1:1.2, 1:2.6, 1:4.3, 1:5.5, 1:6, 1:7.5, 1:9, 1:10, 1:15, 1:20, etc.

[0016] Furthermore, the portion of the sample tube located in the conducting cavity is a straight tube or a spiral tube.

[0017] Furthermore, the constant temperature maintaining unit also includes a temperature regulating mechanism and a temperature sensor. The temperature regulating mechanism is connected to the heat preservation container and the constant temperature medium circulation supply mechanism. The temperature regulating mechanism, the heat preservation container, and the constant temperature medium circulation supply mechanism form a constant temperature medium circuit through which the constant temperature medium can continuously and circulate. The temperature regulating mechanism is at least used to regulate the temperature of the constant temperature medium in the constant temperature medium circuit, and the temperature sensor is at least used to monitor the temperature of the constant temperature medium flowing into and / or out of the heat preservation container.

[0018] Furthermore, the constant temperature medium can be a water bath, an oil bath or a cold medium such as ethanol and propylene glycol.

[0019] A second aspect of the embodiments of the present invention provides a continuous flow magnetic field biological fermentation and catalytic synthesis method, which comprises: Providing the continuous flow magnetic field biological fermentation and catalytic synthesis device; Allowing the liquid in the sample container to continuously and circulate through the sample tube; The magnetic field assembly generates a magnetic field passing through the sample tube, and the angle θ between the direction of the magnetic field lines of the magnetic field and the flow direction of the liquid in the sample tube passing through the magnetic field is 0° to 90°, for example, θ can be 0°, 10°, 15°, 20°, 23°, 32°, 45°, 60°, 75°, 90°, etc.; The temperature of the magnetic field assembly and the liquid in the sample tube is adjusted by the constant temperature maintaining unit.

[0020] Furthermore, the magnetic field is an alternating magnetic field or a static magnetic field, the magnetic field strength of the alternating magnetic field is 0.1 mT~500 mT, the frequency is 0.1 Hz~100 kHz, and the magnetic field strength of the static magnetic field is 0.1 mT~600 mT.

[0021] Furthermore, the flow rate of the liquid in the sample tube is 5 mL / h ~1000 L / h.

[0022] Furthermore, the temperature of the magnetic field assembly and the constant temperature medium is maintained at -40°C~150°C, for example, it can be -40°C, -30°C, -22°C, -10°C, 0°C, 15°C, 25°C, 55°C, 80°C, 100°C, 150°C, etc.

[0023] Furthermore, the constant temperature medium and the feed liquid are driven and flowed by a pump, and the pump may be a centrifugal pump, a horizontal flow pump, a screw pump or a peristaltic pump.

[0024] Compared with the prior art, the advantages of the present invention include: The present invention provides a continuous flow magnetic field biological fermentation and catalytic synthesis device, which can realize the continuous catalytic treatment of the fermentation liquid or reaction medium in the magnetic field under the external circulation state, and at the same time, the distance between the medium in the sample tube and the magnetic field component, the flow direction and the direction of the magnetic field line are limited, so that the sample medium or feed liquid avoids the uneven magnetic field environment and ensures that it is subject to a stable magnetic flux. The application effect is repeatable, so the effect on the biological fermentation or catalytic synthesis reaction can be achieved by regulating the stable magnetic field environment. In addition, a high-energy-density medium-frequency magnetic field can be generated through a special conductive cavity structure and a constant temperature cavity, so the large-scale application of this technology can be realized.

[0025] The present invention realizes efficient, stable and rapid reaction process of microbial fermentation and chemical catalysis exposed to magnetic field. In addition, the angle between the direction of the magnetic field lines and the direction of the flow of the fermentation liquid or the reaction medium is 0-90°. For the charged solutes and free ions in the fermentation liquid or the reaction medium, since the direction of the fluid flow velocity and the direction of the magnetic field lines have a certain angle, the magnitude and direction of the Lorentz force on the charged solutes and free ions in the fluid will show a specific regulation effect due to the angle, and under the action of a stable magnetic flux, the ion exchange and material transfer of the free ions in the reaction substrate in the pores of the fermentation liquid microbial cell membrane or the mutual orientation effect between the components in the reaction medium are promoted. That is, when the charged solutes in the fermentation liquid or reaction medium move in the fluid, they will cause changes in the induced electric field in the surrounding area, and the direction and magnitude of the electric field force are related to the motion state of the solute or ion, and the spatial alternating electric field force caused by the magnetic field changing in different directions is also a vector. The interaction between the two vectors will further produce a synergistic effect on the charged solutes and free ions in the fermentation liquid or reaction medium, thereby affecting the physical and chemical properties of the fermentation culture medium or reaction medium, such as the chemical reaction rate and the diffusion of the solute, thereby improving the growth efficiency, metabolic yield or reaction rate, etc. BRIEF DESCRIPTION OF THE DRAWINGS

[0026] Figure 1 It is a structural schematic diagram of a continuous flow magnetic field biological fermentation and catalytic synthesis device provided in a typical implementation case of the present invention; Figure 2a , Figure 2b This is a partial structural diagram of a continuous flow magnetic field biological fermentation and catalytic synthesis device provided in Example 1 of the present invention; Figure 3a , Figure 3b This is a partial structural diagram of a continuous flow magnetic field biological fermentation and catalytic synthesis device provided in Example 2 of the present invention; Figure 4a , Figure 4b It is a partial structural schematic diagram of a continuous flow magnetic field biological fermentation and catalytic synthesis device provided in Example 3 of the present invention. DETAILED DESCRIPTION

[0027] In view of the deficiencies in the prior art, the inventor of this case has proposed the technical solution of the present invention after long-term research and extensive practice. The technical solution, its implementation process and principle, etc. will be further explained in conjunction with the accompanying drawings and specific implementation cases. It should be noted that the controller, power supply, refrigeration compressor, heater, temperature sensor, etc. used in the present invention are all known to those skilled in the art and can be obtained commercially. The above-mentioned technical features of the present invention and the technical features specifically described in the following (embodiment) can be combined with each other to form a new or preferred technical solution. Due to space limitations, they will not be described one by one here.

[0028] In a more specific implementation, see Figure 1 , a continuous flow magnetic field biological fermentation and catalytic synthesis device, comprising: a heat preservation container 101, x magnetic field components 103, x sample tubes 105, a power supply 110, a controller 111, a temperature adjustment device 112, a constant temperature medium pump 113, a sample pump 114, and a sample container 115; The heat preservation container 101 includes a first shell and x second shells, the first shell and the x second shells are fixedly combined, the first shell and the x second shells are enclosed to form a closed constant temperature cavity, each second shell is enclosed to form a conductive cavity 102 directly connected to the outside, the constant temperature cavity includes a first constant temperature cavity and x second constant temperature cavities 104, the x second constant temperature cavities 104 are surrounded by one of the first constant temperature cavities and are directly connected, each of the second constant temperature cavities 104 is arranged around the periphery of one of the conductive cavities 102 and The second shell is heat-conductingly matched with the conducting cavity 102; the first shell is provided with a constant temperature medium inlet 107 and a constant temperature medium outlet 108, a temperature sensor 106 is provided in the constant temperature medium outlet 108, a constant temperature medium pump 113 is connected with a temperature regulating device 112, and the constant temperature medium pump 113 and the temperature regulating device 112 are also respectively connected with the constant temperature medium inlet 107 and the constant temperature medium outlet 108 via a conduit, and the constant temperature medium pump 113, the temperature regulating device 112 and the constant temperature cavity form a constant temperature medium circuit for continuous and cyclic flow of the constant temperature medium; The temperature adjustment device 112 and the temperature sensor 106 are connected to the controller 111, and the temperature parameters of the constant temperature medium can be adjusted and monitored by the controller 111; x magnetic field components 103 are arranged inside the constant temperature cavity, each magnetic field component 103 surrounds a conductive cavity 102 and is used to generate a magnetic field; A portion of each sample tube 105 is disposed in the conductive cavity 102, and both ends of the sample tube 105 are connected to a sample pump 114 and a sample container 115 via conduits, respectively. The sample pump 114 is also connected to the sample container 115. The sample tube 105, the sample pump 114, and the sample container 115 form a liquid loop for continuous and circulating flow of the liquid. The ion exchange and substance interaction of the liquid flowing through the sample tube 105 in the conductive cavity 102 can be changed by the magnetic field.

[0029] Specifically, the constant temperature medium can be pumped into the constant temperature cavity by the constant temperature medium pump 113, and the constant temperature medium loop can be continuously and circulated, and the magnetic field assembly 103 is immersed in the constant temperature medium. The constant temperature medium is circulated inside and outside the heat preservation container 101 and the magnetic field assembly 103, and the temperature regulating device 112 is used to maintain the working temperature of the constant temperature medium and the magnetic field assembly 103 at -40°C to 150°C. In addition, the constant temperature medium can fill the first constant temperature cavity 104 and the second constant temperature cavity 105, and the second constant temperature cavity 106 can be filled with the constant temperature medium. 4 and the second shell regulate the ambient temperature in the conduction cavity 102. Specifically, the temperature of the magnetic field component 103 and the constant temperature medium is measured by the temperature sensor 106. Specifically, the temperature regulating device 112 can be a refrigeration compressor, a heat exchanger or a circulating air cooler, etc. The constant temperature medium pump 113 can be a centrifugal pump, a horizontal flow pump, a screw pump or a peristaltic pump, etc. The temperature sensor 106 can be a PT100 thermal resistor, a K-type thermocouple or an infrared thermal imaging system, etc. The constant temperature medium can be a water bath, an oil bath or a refrigerant. It should be noted that the constant temperature medium can, on the one hand, dissipate heat and control the temperature of the magnetic field component 103, and on the other hand, can also regulate the temperature of the conduction cavity 102, thereby adjusting the temperature of the fermentation liquid by heat conduction and keeping the temperature of the fermentation liquid constant.

[0030] Specifically, the heat preservation container 101 is a closed structure provided with a constant temperature medium inlet 107 and a constant temperature medium outlet 108. Specifically, the first shell and the second shell can be fixed and closed by welding, gluing, fastener connection and other processes. The first shell can be made of metal material or non-metal material, the conduction cavity 102 has machinability, and the second shell is made of non-metal material with a certain strength. The radial cross-section shape of the conduction cavity 102 can be circular, rectangular or other polygonal. Exemplarily, the metal material can be aluminum, iron or stainless steel, etc., and the non-metal material can be tetrafluoro, PFA, polyethylene or epoxy resin, etc.

[0031] Specifically, the magnetic field assembly can be arranged in the first constant temperature cavity and distributed around the second constant temperature cavity 104, or the magnetic field assembly can be arranged in the second constant temperature cavity 104 and surrounded between the conductive cavities 102. Specifically, the angle between the magnetic field lines of the magnetic field generated by the magnetic field assembly 103 and the axial direction of the sample tube 105 surrounded by the magnetic field assembly 103 is 0° to 90°.

[0032] Specifically, the magnetic field component 103 can be a conductive coil or at least one permanent magnet. Specifically, when the magnetic field component 103 is a conductive coil, it is completely or partially wound around the second constant temperature cavity 104 or the conduction cavity 102. A terminal 109 is provided on the heat preservation container 101. The conductive coil is electrically connected to the controller 111 and the power supply 110 via the terminal 109. The current provided by the power supply 110 causes the conductive coil to generate a magnetic field. Specifically, the wire diameter of the conductive coil is 0.01 mm to 5 mm, and the number of turns of the conductive coil is 10 turns to 30,000 turns. Exemplarily, the conductive coil can be a Helmholtz coil, a solenoid, etc. When the magnetic field component 103 is a pair or more pairs of permanent magnets, the pair or more pairs of permanent magnets are fixed to the second constant temperature cavity 104 or the conduction cavity 102.

[0033] Specifically, the magnetic field component 103 can generate an alternating magnetic field with a magnetic field strength of 0.1 mT ~ 500 mT and a frequency of 0.1 Hz ~ 100 kHz, or generate a static magnetic field with a magnetic field strength of 0.1 mT ~ 600 mT, wherein the alternating magnetic field can be generated by the alternating current passing through the magnetic field component 103 by electrically connecting the magnetic field component 103 with the terminal 109, the controller 111 and the power supply 110; the static magnetic field can be generated by the direct current passing through the magnetic field component 103 by electrically connecting the magnetic field component 103 with the terminal 109, the controller 111 and the power supply 110, or the static magnetic field is generated by a permanent magnet, and the power supply 110 is connected to the controller 111, and the power supply parameters and the magnetic field parameters provided by the magnetic field component can be regulated by the controller 111.

[0034] Specifically, the x magnetic field components 103 should be fixed inside the heat preservation container 101. When x≥2, the x magnetic field components 103 should be arranged in parallel, and the ratio of the outer perimeter of the magnetic field component 103 to the center distance of two adjacent magnetic field components 103 should be (1:0.15)~(1:20). Through such a design, the x magnetic field components 103 can simultaneously perform fermentation and catalytic synthesis treatments, increase the processing capacity of the reaction, but can ensure that the parameters of the magnetic field environment in the x sample tubes 105 are basically the same. If the distance between adjacent magnetic field components 103 is too far, the volume of the entire reaction device will be too large, and the magnetic fields between adjacent magnetic field components 103 will be isolated from each other. If the distance between adjacent magnetic field components 103 is too close, it will affect the heat dissipation of each other. At the same time, the x magnetic field components 103 are placed in parallel, the directions and distributions of the magnetic field lines of the x magnetic field components 103 are the same, and the magnetic fields between the x magnetic field components 103 have an enhancement effect. Otherwise, magnetic fields at different angles or in opposite directions will weaken other magnetic fields.

[0035] It should be noted that the outer perimeter of the magnetic field component 103 refers to the length of the circumferential contour of the magnetic field component 103, which can be specifically understood as the perimeter of the orthographic projection figure formed by the magnetic field component 103 along its own axial direction. For example, when the magnetic field component 103 is a conductive coil, the outer perimeter can be the length of a single-turn annular coil. When the magnetic field component 103 is a single annular permanent magnet, the outer perimeter is the circumferential length of the outer ring of the end face of the annular permanent magnet. When the magnetic field component 103 is a plurality of permanent magnets, the plurality of permanent magnets are distributed in an annular region, and the outer perimeter is the circumferential length of the outer ring of the annular region. The center distance between two adjacent magnetic field components 103 is the vertical distance between the geometric centers of the two adjacent magnetic field components 103. When the two magnetic field components 103 are generally presented as a regular geometric structure, such as a cylindrical structure or a prismatic structure, the center distance between the two adjacent magnetic field components 103 can also be regarded as the straight-line distance between the geometric centers of the two orthographic projection figures formed by the two adjacent magnetic field components 103 along their own axial direction.

[0036] Specifically, the conductive cavity 102 passes through the heat preservation container 101, and the sample tube 105 for circulating the feed liquid (fermentation liquid or synthetic reaction mixture) can all pass through the conductive cavity 102; the sample pump 114 is used to realize the continuous flow of the feed liquid inside the sample tube 105, and the angle θ between the direction of the magnetic lines of force generated by the magnetic field component 103 and the flow direction of the feed liquid in the sample tube 105 is 0~90°. Exemplarily, the sample tube 105 all passes through the conductive cavity 102, and the sample pump 114 is used to pump the feed liquid to be processed into the sample tube 105 and flow continuously. In addition, the feed liquid can be fermented or catalytically reacted in an external sample container 115, and the sample container 115 can be a fermentation tank or a reactor. It should be noted that the portion of the sample tube 105 located in the conductive cavity 102 is a straight tube or a spiral tube. The difference between the straight tube and the spiral tube is that the angle of interaction between the magnetic lines of force and the reaction medium is different. When the sample tube 105 is a spiral tube, the angle θ between the direction of the magnetic lines of force generated by the magnetic field assembly 103 and the flow direction of the liquid in the sample tube 105 is closer to 90°. Moreover, under the same magnetic line distribution conditions, the spiral tube has a longer pipeline and a longer action time, and the fermentation or catalytic reaction is more complete.

[0037] Specifically, the controller 111 can be a PLC controller or an integrated circuit board, etc. The numerical control program used by the controller can be obtained commercially, and the circuit structure in the present invention is implemented using methods or technologies known to technical personnel in this field, and no special limitation is made here.

[0038] Specifically, the continuous flow magnetic field biological fermentation and catalytic synthesis device can be used in the fields of food, biology, life science, environment, materials, chemical industry, medicine, etc.

[0039] Example 1 A method for fermenting Antrodia cinnamomea in a continuous flow magnetic field. In this embodiment, the device for fermenting Antrodia cinnamomea is the continuous flow magnetic field biological fermentation and catalytic synthesis device in Example 1, and its partial structure is as follows Figure 2a , Figure 2b shown.

[0040] The continuous flow magnetic field biological fermentation and catalytic synthesis device used in this embodiment is provided with four magnetic field components 202, and the magnetic field components 202 are solenoids, wherein the wire diameter of the solenoid used is 2.0 mm, and the number of turns of a single solenoid is 600 turns. The four magnetic field components 202 are arranged in parallel and vertically fixed inside the heat preservation container 101, and the ratio of the outer edge circumference of the solenoid to the center distance of the adjacent magnetic field components 202 is 1:0.5; the conductive cavity 201 passes through the heat preservation container 101, and the conductive cavity 201 is made of PFA material (that is, the material of the aforementioned second shell is PFA material), and the solenoids are all wound around the periphery of the second constant temperature cavity 203. All sample tubes 204 pass through the conducting cavity 201, and after the fermentation liquid is pumped in, it flows continuously in the sample tubes 204, and the angle between the direction of the magnetic field lines and the direction of the fermentation liquid flowing in the sample tubes 204 is 0°; a constant temperature oil bath is pumped into the heat preservation container 101, and a refrigeration compressor or a heat exchanger controls the temperature of the constant temperature oil bath. A centrifugal pump is used to realize the circulation of the constant temperature oil bath inside and outside the first constant temperature cavity, the second constant temperature cavity and the magnetic field assembly of the heat preservation container 101.

[0041] A method for fermenting Antrodia camphorata in a continuous flow magnetic field, specifically comprising: (1) Take the preserved Antrodia camphorata strain and inoculate it into the slant culture medium, and culture it at 25°C for two weeks to activate the strain; (2) Take 1 cm 2 The activated slant bacterial block in step (1) was cultured in 100 mL of seed culture solution at 25°C for one week to prepare seed solution; (3) The Antrodia camphorata seed solution prepared in step (2) was inoculated into a liquid culture medium at an inoculation rate of 8%, and the fermentation liquid was pumped into a continuous flow magnetic field biological fermentation and catalytic synthesis device as the fermentation liquid. The device parameters were set as follows: a 6 A excitation current was applied to the solenoid so that the magnetic field component 202 generated an alternating magnetic field of 20 mT and 500 Hz; the fermentation temperature of the fermentation liquid was set to 25°C, and a constant temperature oil bath was controlled by a centrifugal pump and a refrigeration compressor so that the fermentation liquid circulated into and out of the insulation container, and the temperature of the constant temperature oil bath was maintained at 25°C; and the flow rate in the sample tube was 5 L / h, and the constant temperature culture was carried out for 5 days.

[0042] After completion, the growth of Antrodia camphorata was observed and the biomass and biomass increase rate of Antrodia camphorata fermentation liquid were calculated.

[0043] The specific calculation method is as follows: Biomass: Take 100 mL of fermentation broth, centrifuge at 6000×g for 8 min, collect mycelium, wash with distilled water 3 times, and dry in a 50 ℃ oven to constant weight. The biomass per unit volume of Antrodia camphorata fermentation broth (mg / mL) = mycelium mass after drying to constant weight / fermentation broth volume; The results showed that: in this embodiment, the biomass per unit volume of Antrodia camphorata fermentation liquid reached 0.742 mg / mL.

[0044] Comparative Example 1 A method for fermenting Antrodia camphorata, the difference between comparative example 1 and example 1 is that the ratio of the outer perimeter of the solenoid to the center distance of the adjacent magnetic field components is 1:0.14, that is, the solenoid is wound into a right triangle appearance, and the adjacent magnetic field components are tightly fitted.

[0045] After the end, the growth of Antrodia camphorata was observed and the biomass of Antrodia camphorata fermentation liquid was calculated. The results showed that the biomass per unit volume of Antrodia camphorata fermentation liquid in Comparative Example 1 was only 0.326 mg / mL.

[0046] The continuous flow magnetic field biological fermentation and catalytic synthesis device of the present invention is adopted, and the biomass of Example 1 is significantly increased by 127.61% compared with the close fit of the magnetic field assembly in Comparative Example 1. The fermentation broth contains a large amount of carbon source, nitrogen source, inorganic salt and water, which have antimagnetic effects, and a weak reverse orientation of the electron cloud magnetic moment occurs under the magnetic field environment. The pores of the cell membrane of the Antrodia cinnamomea tissue change under this effect, promoting material transfer, and the action of active enzymes and substrates, so that the fermentation of Antrodia cinnamomea is strengthened and the biomass is increased. In Comparative Example 1, the magnetic field assembly is closely fitted, the magnetic field assembly heats up significantly, the temperature of the constant temperature oil bath rises sharply, and the sample in the sample tube produces obvious temperature fluctuations. At the same time, the magnetic field assembly is too close, and the magnetic lines of force generated interfere with each other, resulting in the inability to gather a high energy density magnetic field, further inhibiting the fermentation of Antrodia cinnamomea.

[0047] Example 2 A method for producing alcohol by continuous flow magnetic field fermentation. The device for alcohol fermentation in this embodiment is the continuous flow magnetic field biological fermentation and catalytic synthesis device in embodiment 1, such as Figure 3a , Figure 3bAs shown, the continuous flow magnetic field biological fermentation and catalytic synthesis device used in this embodiment is provided with three magnetic field components 302, and the magnetic field components 302 are solenoids, wherein the wire diameter of the solenoid used is 2.6 mm, and the number of turns of a single solenoid is 450 turns. The three magnetic field components 302 are arranged in parallel and vertically fixed inside the heat preservation container, and the ratio of the circumference of the outer edge of the solenoid to the center distance of the adjacent magnetic field components is 1:2; the conductive cavity 301 passes through the heat preservation container and is made of PFA material. The conductive cavity is provided with a second constant temperature cavity 303, and the solenoids are all wound around the periphery of the second constant temperature cavity 303. All sample tubes 304 pass through the conducting cavity 301, and after the fermentation liquid is pumped in, it flows continuously in the sample tube 304, and the angle 305 between the magnetic field lines and the flow direction of the fermentation liquid in the sample tube 304 is 15°; a constant temperature water bath is pumped into the thermal insulation container, and a refrigeration compressor or a heat exchanger controls the constant temperature water bath, and a peristaltic pump is used to realize the circulation of the constant temperature water bath inside and outside the first constant temperature cavity, the second constant temperature cavity and the magnetic field assembly of the thermal insulation container.

[0048] A method for continuous flow magnetic field fermentation of alcohol, specifically comprising: (1) Activation of strains: Take the preserved yeast strain, inoculate it into the slant medium of malt juice, and culture it at a constant temperature of 28°C for two days to activate the strain; (2) Raw material pretreatment: Take the saccharification liquid of industrial alcohol production with a sugar content of 12°Bx, sterilize it, add the activated yeast strain at an inoculation rate of 3%, mix well, and obtain the initial fermentation liquid; (3) The initial fermentation liquid in step (2) was pumped into a continuous flow magnetic field fermentation and catalytic device, and the device parameters were set: a 7 A excitation current was applied to the solenoid so that the magnetic field component generated a static magnetic field of 10 mT in the conduction cavity; the fermentation temperature of the fermentation liquid was set to 28 °C, the refrigeration compressor controlled the temperature of the constant temperature water bath, and the peristaltic pump made the circulating flow flow into and out of the insulation container, and the temperature of the constant temperature water bath was maintained at 32 °C; and the flow rate in the sample tube was 4 L / h, and the constant temperature culture was carried out for 5 days.

[0049] After completion, observe the alcohol fermentation and use an alcohol meter to measure the alcohol content of the fermented liquid.

[0050] The results show that: in this embodiment, the alcohol content of the fermentation liquid is 9.74% Vol.

[0051] Comparative Example 2 A method for alcohol fermentation, the difference between comparative example 2 and example 2 is that: the fermentation liquid is pumped into a continuous flow magnetic field fermentation and catalysis device, and the angle between the magnetic field lines and the direction of the fermentation liquid flowing in the sample tube is 105°, and the magnetic field strength of the device is 0.05 mT.

[0052] After completion, the alcohol content of the fermentation liquid was determined by referring to the method of Example 2. The results showed that the alcohol content of the fermentation liquid of Comparative Example 2 was 8.86% Vol.

[0053] By using the continuous flow magnetic field biological fermentation and catalytic synthesis device of the present invention, compared with Comparative Example 2, the alcohol content of the fermented liquid in Example 2 is increased by 9.93%. Under the appropriate magnetic field strength and the angle between the magnetic field direction and the flow direction of the fermented liquid, the interaction between the fermented liquid and the substrate is enhanced, the water molecule clusters are dispersed, and alcohol fermentation and product synthesis are promoted. However, the magnetic field strength of Comparative Example 2 is relatively small, and the angle between the flow direction of the fermented liquid and the magnetic field direction is too large, and the disturbance between different components is too severe, which causes some components in the fermented liquid to produce more spin polarization or exposure of hydrophobic groups, and the interaction is reduced. There is no obvious directional effect on the components in the fermented liquid and even side effects are produced. The magnetic field energy utilization rate is low, and the fermentation alcohol efficiency is low.

[0054] Example 3 A method for hydrolyzing polyferric sulfate, wherein the device for hydrolyzing polyferric sulfate in Example 4 is the continuous flow magnetic field biological fermentation and catalytic synthesis device in Example 1, such as Figure 4a , Figure 4b As shown, the continuous flow magnetic field biological fermentation and catalytic synthesis device used in this embodiment is provided with two magnetic field components 402, and the magnetic field components 402 are solenoids, wherein the wire diameter of the solenoid used is 2 mm, and the number of turns of a single solenoid is 800 turns. The two magnetic field components 402 are arranged in parallel and vertically fixed inside the heat preservation container, and the ratio of the circumference of the outer edge of the solenoid to the center distance of the adjacent magnetic field components is 1:1; the conductive cavity 401 passes through the heat preservation container and is made of epoxy resin. The conductive cavity 401 is provided with a second constant temperature cavity 403, and the solenoids are all wound around the periphery of the second constant temperature cavity 403. All sample tubes 404 pass through the conducting cavity 401, and after the reaction liquid is pumped in, it flows continuously in the sample tubes 404, and the angle 405 between the magnetic field lines and the flow direction of the reaction liquid in the sample tubes 404 is 90°; a constant temperature oil bath is pumped into the insulation container, and a refrigeration compressor or a heat exchanger controls the constant temperature oil bath, and a centrifugal pump is used to realize the circulation of the constant temperature oil bath inside and outside the first constant temperature cavity, the second constant temperature cavity and the magnetic field assembly of the insulation container.

[0055] A method for continuous flow magnetic field catalytic hydrolysis of polyferric sulfate, specifically comprising: (1) Prepare 100 mg / L polyferric sulfate liquid, wherein the ferric sulfate content is 20% and the pH is 2.6; (2) The polyferric sulfate liquid of step (1) is pumped into a continuous flow magnetic field biofermentation and catalytic synthesis device as a reaction medium, and the device parameters are set: a 9.5 A excitation current is applied to the solenoid, so that the magnetic field component generates an alternating magnetic field of 30 mT, 800 Hz in the conduction cavity; the hydrolysis temperature of the reaction medium is set to 65 °C, and a centrifugal pump and a heat exchanger control a constant temperature oil bath so that it circulates into and out of the insulation container, and the temperature of the constant temperature oil bath is maintained at 65 °C; and the flow rate in the sample tube is 10 L / h, and the cyclic hydrolysis is performed for 2 h; After completion, observe the hydrolysis of the reaction medium, measure the UV absorption spectrum, and calculate the Fe(OH)2 + The integral area of ​​the characteristic absorption peak indicates the change of Fe(OH)2 + The results showed that after 2 h of cyclic hydrolysis, the integrated area of ​​the ultraviolet absorption peak of the reaction medium at 300 nm was 76.4.

[0056] Comparative Example 3 A method for hydrolyzing polyferric sulfate. The difference between the device used in comparative example 3 for hydrolyzing polyferric sulfate and the continuous flow magnetic field biological fermentation and catalytic synthesis device used in Example 3 is that: the reaction liquid is pumped into the continuous flow magnetic field fermentation and catalytic device, and the magnetic field direction of the magnetic field is at an angle of 150° to the direction in which the reaction liquid flows in the sample tube, the magnetic field strength of the device is 600 mT, and the frequency is 1500 Hz.

[0057] After the reaction, the hydrolysis of the reaction medium was observed, the UV absorption spectrum was measured, and the Fe(OH)2 + The results show that after 2 h of cyclic hydrolysis, the integrated area of ​​the ultraviolet absorption peak of the reaction medium at 300 nm is 48.2.

[0058] By using the continuous flow magnetic field biological fermentation and catalytic synthesis device of the present invention, under the conditions of suitable magnetic field strength and angle between the magnetic field direction and the flow direction of the feed liquid, the sample reaction temperature can be controlled, and the metal salt compounds have obvious electron cloud orientation under the magnetic field, Fe 3+ The charged solutes and free ions are acted upon by the Lorentz force, which strengthens the binding force of the hydrated ion compounds, the hydrogen bonding ability of the water molecules, and the mass transfer rate. 3+Gradually combined with hydroxyl groups, promoting the transformation of low-polymer state to high-polymer state hydrated compounds, and promoting hydrolysis reaction. In Comparative Example 3, the magnetic field intensity is relatively high, and the angle between the flow direction of the reaction liquid and the magnetic field direction is too large, which causes too much disturbance between different components. At the same time, the isoelectric solutes and free ions in the polyferric sulfate solution are highly spin-polarized, and the interaction between the components is offset, inhibiting the growth of high-polymer substances, and weakening the hydrolysis effect. The integrated area of ​​the ultraviolet absorption peak at 300 nm in Comparative Example 3 is reduced compared with that in Example 3, that is, the Fe(OH)2 produced by hydrolysis + The content decreased by 36.9%.

[0059] It should be noted that the above are only some embodiments of the present invention. The inventors of this case also refer to Embodiment 1, Embodiment 2 and Embodiment 3, and change the angle θ between the direction of the magnetic field lines of the magnetic field generated by the magnetic field component passing through the sample tube and the flow direction of the slurry in the sample tube passing through the magnetic field, the ratio of the outer perimeter of the magnetic field component to the center distance between two adjacent magnetic field components, the flow rate of the slurry in the sample tube, the temperature of the magnetic field component and the constant temperature medium, the type of magnetic field, the magnetic field strength / frequency of the alternating magnetic field, and the magnetic field strength of the static magnetic field. Specifically, the angle θ between the direction of the magnetic field lines of the magnetic field generated by the magnetic field component passing through the sample tube and the flow direction of the slurry in the sample tube passing through the magnetic field is set to 0°, 10°, 15°, 20°, 23°, 32°, 45°, 60°, 75°, 90°, etc., and the temperature of the magnetic field component and the constant temperature medium is maintained at -40°C, -30°C, -22°C, -10°C, 0°C, 15°C, 25°C, 55°C, 80°C, etc. ℃, 100 ℃, 150 ℃, etc., set the ratio of the outer perimeter of the magnetic field assembly to the center distance between two adjacent magnetic field assemblies to 1:0.15, 1:0.3, 1:1.2, 1:2.6, 1:4.3, 1:5.5, 1:6, 1:7.5, 1:9, 1:10, 1:15, 1:20, etc., select an alternating magnetic field or a static magnetic field, the magnetic field strength of the alternating magnetic field is 0.1 mT, 1 mT, 10 mT, 50 mT, 100 mT, 260 mT, 350 mT, 400 mT, 420mT, 500 mT, etc., the magnetic field strength of the static magnetic field is 0.1 mT, 3 mT, 55 mT, 120 mT, 280 mT, 360 mT, 400mT, 500 mT, 600 mT, etc., the flow rate of the liquid in the sample tube is 5 mL / h, 20 L / h, 100 L / h, 350 L / h, 420 L / h, 560 L / h, 600 L / h, 750 L / h, 900 L / h, 1000 L / h, etc., to obtain a series of embodiments, and verify the effects of different magnetic field conditions on the fermentation / synthesis reactions of fermentation of Antrodia camphorata, fermentation to produce alcohol, hydrolysis of polyferric sulfate and other materials, and the results are basically close to those of Examples 1, 2 and 3.

[0060] It should be understood that the above embodiments are only for illustrating the technical concept and features of the present invention, and their purpose is to enable people familiar with the technology to understand the content of the present invention and implement it accordingly, and they cannot be used to limit the protection scope of the present invention. Any equivalent changes or modifications made according to the spirit of the present invention should be included in the protection scope of the present invention.

Claims

1. A continuous flow magnetic field biological fermentation and catalytic synthesis device, characterized in that: include: A constant temperature maintenance unit, comprising a sealed heat preservation container and a constant temperature medium circulation supply mechanism, wherein the heat preservation container has a sealed constant temperature cavity and x conductive cavities penetrating the container, wherein the conductive cavity is surrounded by the constant temperature cavity and is heat-conductingly matched with the constant temperature cavity, and the constant temperature medium circulation supply mechanism is connected with the constant temperature cavity to form a constant temperature medium circuit for continuous and circulating constant temperature medium; A reaction generating unit, comprising a sample container and y sample tubes, wherein the sample container is at least used to contain a feed liquid and provide a required environment for fermentation culture or catalytic synthesis reaction of the feed liquid, each of the sample tubes and the sample container form a feed liquid loop for continuous flow of the feed liquid, and at least a portion of each of the sample tubes is disposed in a conduction cavity and is thermally coordinated with the conduction cavity; A magnetic field generating unit comprises y magnetic field components, wherein the y magnetic field components are arranged in the constant temperature cavity, each of the magnetic field components is correspondingly distributed around one of the conductive cavities, and the magnetic field components are at least used to generate a magnetic field passing through the conductive cavity, and the angle between the direction of the magnetic field lines of the magnetic field generated by the magnetic field components and the axial direction of the sample tube passing through the magnetic field components is 0°~90°; the magnetic field can change the physical and chemical parameters of the medium or liquid in the sample tube, x≥y≥1, and x and y are positive integers.

2. The continuous flow magnetic field biological fermentation and catalytic synthesis device according to claim 1, characterized in that: The constant temperature maintaining unit further includes y heat dissipation structures, which are arranged in the constant temperature cavity. Each of the heat dissipation structures is arranged around a conductive cavity and cooperates with the conductive cavity for thermal conductivity.

3. The continuous flow magnetic field biological fermentation and catalytic synthesis device according to claim 2, characterized in that: The constant temperature cavity includes a first constant temperature cavity and y second constant temperature cavities, the y second constant temperature cavities are surrounded by and connected to the first constant temperature cavity, each of the second constant temperature cavities is arranged around a conductive cavity and cooperates with the conductive cavity for heat conduction, and the second constant temperature cavity and the constant temperature medium located in the second constant temperature cavity serve as the heat dissipation structure; And / or, the thermal insulation container includes a first shell and x second shells, the first shell and the x second shells are fixedly combined, the first shell and the x second shells enclose the constant temperature cavity, each second shell encloses the conductive cavity, wherein the second shell is formed of non-metallic material.

4. The continuous flow magnetic field biological fermentation and catalytic synthesis device according to claim 2 or 3, characterized in that: The magnetic field components are distributed around the heat dissipation structure, or the magnetic field components are arranged between the heat dissipation structure and the conduction cavity.

5. The continuous flow magnetic field biological fermentation and catalytic synthesis device according to claim 2 or 3, characterized in that: The magnetic field component includes a conductive coil, the conductive coil is wound around the periphery of the conductive cavity, and the conductive coil generates the magnetic field by connecting to a power source; or, the magnetic field component includes at least one permanent magnet; Preferably, the wire diameter of the conductive coil is 0.01 mm to 5 mm; and / or the number of turns of each conductive coil is 10 turns to 30,000 turns; Preferably, the magnetic field is an alternating magnetic field or a static magnetic field, the alternating magnetic field is generated by passing alternating current into the conductive coil, the static magnetic field is generated by passing direct current into the conductive coil, or the static magnetic field is generated by the permanent magnet.

6. The continuous flow magnetic field biological fermentation and catalytic synthesis device according to claim 5, characterized in that: The y magnetic field components are arranged in parallel, and the ratio of the outer perimeter of the magnetic field component to the center distance between two adjacent magnetic field components is (1:0.15) to (1:20); And / or, the portion of the sample tube located in the conducting cavity is a straight tube or a spiral tube.

7. The continuous flow magnetic field biological fermentation and catalytic synthesis device according to claim 1, characterized in that: The constant temperature maintaining unit also includes a temperature regulating mechanism and a temperature sensor. The temperature regulating mechanism is connected to the heat preservation container and the constant temperature medium circulation supply mechanism. The temperature regulating mechanism, the heat preservation container, and the constant temperature medium circulation supply mechanism form a constant temperature medium circuit through which the constant temperature medium can continuously and circulate. The temperature regulating mechanism is at least used to regulate the temperature of the constant temperature medium in the constant temperature medium circuit, and the temperature sensor is at least used to monitor the temperature of the constant temperature medium flowing into and / or out of the heat preservation container.

8. A continuous flow magnetic field biological fermentation and catalytic synthesis method, characterized in that: include: Provide a continuous flow magnetic field biological fermentation and catalytic synthesis device according to any one of claims 1 to 7; Allowing the liquid in the sample container to continuously and circulate through the sample tube; The magnetic field assembly generates a magnetic field passing through the sample tube, and the angle θ between the direction of the magnetic field lines and the flow direction of the liquid in the sample tube passing through the magnetic field is 0° to 90°; The temperature of the magnetic field assembly and the liquid in the sample tube is adjusted by the constant temperature maintaining unit.

9. The continuous flow magnetic field biological fermentation and catalytic synthesis method according to claim 8, characterized in that: The magnetic field is an alternating magnetic field or a static magnetic field. The magnetic field strength of the alternating magnetic field is 0.1 mT~500 mT and the frequency is 0.1 Hz~100 kHz. The magnetic field strength of the static magnetic field is 0.1 mT~600 mT.

10. The continuous flow magnetic field biological fermentation and catalytic synthesis method according to claim 8, characterized in that: The flow rate of the liquid in the sample tube is 5 mL / h ~1000 L / h.

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