Online calibration device for microplate incubator

Abstract

The invention provides an online calibration device for a microplate incubator, and relates to the field of incubator calibration. The online calibration device for the microplate incubator comprises a temperature measurement unit, a wireless temperature measurement terminal structure, a wireless temperature measurement collector and an upper computer. According to the online calibration device for the microplate incubator, key technologies such as the temperature and the rotating speed of the microplate incubator are researched, the calibration device for the microplate incubator with the function of calibrating the temperature and the rotating speed of the microplate incubator is developed, and the online calibration device for the microplate incubator consists of a temperature measuring unit and a rotating speed measuring unit, a control system and data acquisition and analysis software are independently developed, the system is composed of a group of miniature wireless temperature measurement probes, a wireless receiver and special test and analysis software, the performance of the system reaches or is superior to that of similar products at home and abroad, and meanwhile, the system meets the calibration specification of microplate incubators. Indexes such as temperature deviation, temperature fluctuation and temperature uniformity of the instrument can be automatically calculated, and a calibration report conforming to calibration specifications is generated.

Description

Technical Field

[0001] The invention relates to the technical field of incubator calibration, in particular to an online calibration device for a microplate incubator. Background Art

[0002] The microplate incubator uses an advanced temperature control system that can adjust the temperature with high precision. Since microorganisms, cells or enzyme reactions are all affected by temperature, precise temperature control technology can ensure the accuracy and reproducibility of experimental data. The microplate incubator uses high-performance temperature sensors and PID temperature controllers to achieve infinitely variable speeds, and the stability and control accuracy of temperature are at a high level. During long-term operation, the temperature fluctuation is very small, allowing microorganisms and cells to grow and reproduce smoothly at a constant temperature. The microplate incubator can not only be used for conventional applications such as microbial fermentation, cell culture, and enzyme reactions, but also for special experiments such as PCR amplification, gene cloning, and DNA sequencing. Various types of experiments can be effectively handled by the constant temperature incubator. The microplate incubator is simple and easy to operate. Users can set and display parameters through the touch screen to achieve automatic control and recording. In addition, the device has a compact structure and occupies little space, making it very suitable for placement in the laboratory. The microplate incubator has multiple safety protection measures during use. For example, the device has an overheating protection function, which automatically stops heating when the set temperature exceeds a certain range. At the same time, it also has safety measures such as electronic door locks and sound and light alarms to facilitate experimental operations and management. Currently, there is an urgent need to study key technologies such as the temperature and speed of the microplate incubator, and to develop a microplate incubator calibration device with the function of calibrating the temperature and speed of the microplate incubator. Summary of the invention

[0003] The purpose of the present invention is to provide an online calibration device for a microplate incubator, which solves the problems raised in the above-mentioned background technology.

[0004] Technical Solution

[0005] To achieve the above objectives, the present invention is implemented through the following technical solutions: an online calibration device for a microplate incubator, comprising a temperature measuring unit, a wireless temperature measurement terminal structure, a wireless temperature measurement collector and a host computer. The temperature measuring unit has the same appearance as the microplate and can be directly placed on the microplate incubator. Five temperature measurement points are distributed in the temperature measuring unit. The temperature detection unit is composed of a copper cap, a temperature sensor, a base, a circuit board, and a protection board. The temperature probe is placed between the copper cap and the base to form a temperature probe. The entire probe is directly fixed on the PCB board, and a protection board is added to protect the circuit. The copper cap is made of brass, and the overall structure uses PCB as the substrate. The wireless transmission mode and the rotation speed are detected by laser. The laser sensor is placed on a tripod, and the laser probe is aimed at the device to be tested for measurement, such asFigure 8 As shown, it includes a laser transmitting and receiving window, a tripod mounting hole and a charging port; the wireless temperature measurement terminal structure is mainly composed of an MCU main control module, a wireless transceiver module, an amplification and conditioning module, a high-precision PT100 temperature sensor, a reset circuit and a power module. The temperature control accuracy of the microplate incubator is generally ±0.3℃, ±0.5℃, and the instrument indication resolution is generally 0.1℃. The PT100 temperature sensor adopts a four-wire connection method, which eliminates the influence of the temperature sensing line on the measured temperature result and improves the measurement accuracy. The temperature measurement accuracy is 0.05℃. The wireless temperature measurement collector consists of an MCU main control module, a power management module, a reset circuit, a wireless transceiver module and a USB serial port interface. The host computer uses the USB serial port The interface sends control commands to the temperature collector, and the temperature collector sends the control commands to each wireless temperature measurement terminal through the wireless transceiver module to configure the measurement parameters of the wireless temperature measurement terminal. The host computer uses labview to design specific functions. Labview provides many virtual controls similar to multimeters and oscilloscopes to complete data reception, processing and display. Labview is used to distinguish the transmitted data, display data in real-time charts, record relevant data during the test, and control each sub-module to perform specified functions. At the same time, the host computer supports online calibration parameters and can send the calibrated parameters to the lower computer through instructions to realize the function of online real-time debugging of the equipment.

[0006] Furthermore, when calibrating the temperature sensor, it is necessary to select a temperature sensor with a fast response speed and a consistent response curve. When a step change occurs in the temperature, the time required for the output of the thermal resistor to change to 50% of the step change is the thermal response time T0.5. A thin film platinum resistor is used to encapsulate the PT100 temperature sensor, T0.5<0.9S, and the temperature measurement range is: -50~400(℃).

[0007] Furthermore, the labview is a program development environment that supports functions such as data acquisition, TCP / IP, GPIB, serial port control, data analysis, data display and data storage.

[0008] Furthermore, the host computer software also performs specified operations on the data and generates a report in a specified format for output.

[0009] The present invention provides an online calibration device for a microplate incubator, which has the following beneficial effects:

[0010] The microplate incubator online calibration device, this study studies the key technologies such as the temperature and rotation speed of the microplate incubator, and develops a microplate incubator calibration device with the function of calibrating the temperature and rotation speed of the microplate incubator. It is composed of a temperature measurement unit and a rotation speed measurement unit, and independently develops a control system and data acquisition and analysis software. The system consists of a group of miniature wireless temperature probes, wireless receivers and special test and analysis software. The system performance reaches or exceeds similar products at home and abroad, and meets the "Microplate Incubator Calibration Specifications". It can automatically calculate the instrument's temperature deviation, temperature fluctuation, temperature uniformity and other indicators, and generate a calibration report that meets the calibration specifications. BRIEF DESCRIPTION OF THE DRAWINGS

[0011] Figure 1 are the parameters of the microplate incubator of the present invention;

[0012] Figure 2 This is the automatic ELISA preliminary screening process of the present invention;

[0013] Figure 3 It is a thermal analysis of the microplate in the incubator of the present invention;

[0014] Figure 4 It is a detection schematic diagram of the present invention;

[0015] Figure 5 This is a distribution diagram of detection points of the present invention;

[0016] Figure 6 It is a structural diagram of the present invention;

[0017] Figure 7 It is a detection schematic diagram of the present invention;

[0018] Figure 8 This is the appearance diagram of the laser probe of the present invention;

[0019] Fig. 9 It is the overall block diagram of the circuit principle of the present invention;

[0020] Fig.10 This is a circuit schematic diagram of the microplate incubator calibration device of the present invention;

[0021] Fig.11 The wireless temperature measurement terminal structure of the present invention;

[0022] Fig.12 The wireless temperature collector of the present invention;

[0023] Fig.13 The WIFI communication module of the present invention;

[0024] Fig.14 It is a schematic diagram of the functional structure of the host computer of the present invention.

[0025] Among them, there are 1 copper cap, 2 temperature sensors, 3 bases, 4 circuit boards, 5 protection boards, 1 laser transmitting and receiving window, 2 tripod mounting holes, and 3 charging ports. DETAILED DESCRIPTION

[0026] like Figure 1-14 As shown, an embodiment of the present invention provides an online calibration device for a microplate incubator, including a temperature measuring unit, a wireless temperature measuring terminal structure, a wireless temperature measuring collector and a host computer. The appearance of the temperature measuring unit is consistent with that of the microplate and can be directly placed on the microplate incubator. Figure 5 As shown in Figure 1, there are 5 temperature measurement points distributed in the temperature measurement unit. Figure 6 As shown, the temperature detection unit consists of a copper cap 1, a temperature sensor 2, a base 3, a circuit board 4, and a protection board 5. The temperature probe is placed between the copper cap 1 and the base 3 to form a temperature probe. The entire probe is directly fixed on the PCB board, and a protection board is added to protect the circuit. The copper cap 1 is made of brass, which has good thermal conductivity and fast temperature response. The overall structure uses PCB as the base, wireless transmission mode, and a light and simple structure without multiple components. The rotation speed is detected by laser. The laser sensor is placed on a tripod, and the laser probe is aimed at the device to be tested for measurement. The measurement is simple and convenient. Figure 8 As shown in the figure, 1 is the laser transmitting and receiving window, 2 is the tripod mounting hole, and 3 is the charging port; the laser probe is compact in size, easy to install, and rechargeable, and does not require an external power supply. The structure of the wireless temperature measurement terminal is shown in 11, which is mainly composed of an MCU main control module, a wireless transceiver module, an amplification and conditioning module, a high-precision PT100 temperature sensor, a reset circuit, and a power module. At present, the temperature control accuracy of the microplate incubator is generally ±0.3℃ and ±0.5℃, and the instrument indication resolution is generally 0.1℃. The PT100 temperature sensor adopts a four-wire connection method, which eliminates the influence of the temperature sensing line on the temperature measurement result and improves the measurement accuracy. Its temperature measurement accuracy is 0.05℃. When calibrating the temperature sensor, you need to select a temperature sensor with fast response speed and consistent response curve. When the temperature changes step by step, the time required for the output of the thermal resistor to change to 50% of the step change is the thermal response time T0.5. The PT100 temperature sensor encapsulated with a thin film platinum resistor has a T0.5<0.9S and a fast response speed. The temperature measurement range is -50~400℃, which is sufficient to meet the measurement needs. The wireless temperature collector consists of an MCU main control module, a power management module, a reset circuit, a wireless transceiver module and a USB serial port interface. Its main structure is as follows: Fig.12As shown, the host computer sends control commands to the temperature collector through the USB serial port interface. The temperature collector sends the control commands to each wireless temperature measurement terminal through the wireless transceiver module to configure the measurement parameters of the wireless temperature measurement terminal. The host computer uses LabVIEW to design specific functions. LabVIEW is a program development environment developed by National Instruments (NI) of the United States. It supports data acquisition, TCP / IP, GPIB, serial port control, data analysis, data display and data storage. Many virtual controls such as multimeters and oscilloscopes provided by LabVIEW can help us complete the reception, processing and display of data. We use LabVIEW to distinguish the transmitted data, display the data in real-time charts, record the relevant data during the test, and control each submodule to perform the specified function. At the same time, the host computer supports online calibration parameters, and can send the calibrated parameters to the lower computer through instructions to realize the function of online real-time debugging of the equipment. At the same time, the host computer software also performs specified operations on the data and generates reports in a specified format for output.

Claims

1. An online calibration device for a microplate incubator, comprising a temperature measurement unit, a wireless temperature measurement terminal structure, a wireless temperature measurement collector and a host computer, characterized in that: The temperature measuring unit has the same appearance as the microplate and can be directly placed on the microplate incubator. Five temperature measuring points are distributed in the temperature measuring unit. The temperature detection unit is composed of a copper cap (1), a temperature sensor (2), a base (3), a circuit board (4), and a protection board (5). The temperature probe is placed between the copper cap (1) and the base (3) to form a temperature probe. The entire probe is directly fixed on the PCB board, and a protection board is added to protect the circuit. The copper cap (1) is made of brass. The overall structure uses PCB as the substrate. The wireless transmission mode and the rotation speed are detected by laser. The laser sensor is placed on a tripod. The laser probe is aligned with the device to be tested for measurement, as shown in FIG8 . The temperature measuring unit includes a laser transmitting and receiving window (1), a tripod mounting hole (2), and a charging port (3). The wireless temperature measurement terminal structure is mainly composed of an MCU main control module, a wireless transceiver module, an amplification and conditioning module, a high-precision PT100 temperature sensor, a reset circuit, and a power supply module. The temperature control accuracy of the microplate incubator is generally ±0.3°C, ±0.5°C, and the instrument indication resolution is generally 0. 1℃, the PT100 temperature sensor adopts a four-wire connection method, which eliminates the influence of the temperature sensing line on the measured temperature results and improves the measurement accuracy. Its temperature measurement accuracy is 0.05℃. The wireless temperature measurement collector consists of an MCU main control module, a power management module, a reset circuit, a wireless transceiver module and a USB serial port interface. The host computer sends control commands to the temperature collector through the USB serial port interface. The temperature collector sends the control commands to each wireless temperature measurement terminal through the wireless transceiver module, and configures the measurement parameters of the wireless temperature measurement terminal. The host computer uses labview to design specific functions. Labview provides many virtual controls such as multimeters and oscilloscopes to complete data reception, processing and display. Labview is used to distinguish the transmitted data, display data in real-time charts, record relevant data during the test, and control each sub-module to perform specified functions. At the same time, the host computer supports online calibration parameters, and can send the calibrated parameters to the lower computer through instructions to realize the function of online real-time debugging of the equipment.

2. The microplate incubator online calibration device according to claim 1, characterized in that: When calibrating the temperature sensor, it is necessary to select a temperature sensor with a fast response speed and a consistent response curve. When a step change occurs in the temperature, the time required for the output of the thermal resistor to change to 50% of the step change is the thermal response time T0.

5. A thin film platinum resistor is used to encapsulate the PT100 temperature sensor, T0.5<0.9S, and the temperature measurement range is: -50~400(℃).

3. The microplate incubator online calibration device according to claim 1, characterized in that: The labview is a program development environment that supports functions such as data acquisition, TCP / IP, GPIB, serial port control, data analysis, data display and data storage.

4. The microplate incubator online calibration device according to claim 1, characterized in that: The host computer software also performs specified operations on the data and generates reports in a specified format for output.

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