Straw feed microbial agent and application thereof in preparation of feed by straw fermentation

Abstract

The invention discloses a straw feed microbial agent and application of the straw feed microbial agent in preparation of feed through straw fermentation, and belongs to the technical field of agricultural solid waste treatment. By optimizing the composition of phanerochaete chrysosporium, lactobacillus reuteri, lactobacillus paracasei, bacillus subtilis and bacillus cereus in the flora preparation, the obtained wheat straw microbial agent has the advantages that compared with the conventional straw yellow silage feed in the application of fermenting wheat to prepare feed, the yield of the wheat straw microbial agent is increased; according to the method, the content of crude protein is increased by 28.79%, the content of acetic acid is increased by 42.67%, the content of propionic acid is increased by 28.31%, the content of lactic acid is increased by 103.15%, the content of phosphorus element is increased by 33.19%, the content of calcium element is increased by 34.30%, the palatability and nutritional value of the straw feed are remarkably improved, and the method has the advantages of being low in cost, easy and convenient to operate, capable of shortening the preparation period of the wheat straw feed and the like.

Description

Technical Field

[0001] The invention relates to straw feed preparation, belongs to the technical field of agricultural solid waste treatment for development and utilization of straw feed resources, and specifically relates to a straw feed microbial agent and application thereof in preparing feed by straw fermentation. Background Art

[0002] With the booming development of global animal husbandry, the demand for feed resources is increasing day by day, the supply of traditional feed raw materials is gradually stretched, and the price is rising. At the same time, crop straw, as a major by-product of agricultural production, has a very considerable output. Microbial fermentation of straw to prepare feed has many advantages. First, it can improve the nutritional value. The straw itself is rich in cellulose, hemicellulose and lignin, but the crude protein content is low and the digestibility is poor, making it difficult for livestock to directly and efficiently use it. With the help of microbial fermentation, lactic acid and various enzymes can be produced to decompose straw macromolecules into small molecule sugars, amino acids, etc., to improve palatability and digestibility. At the same time, it can also increase the crude protein content of feed, increase livestock feed intake, and improve growth performance. Second, it can reduce feed costs. Straw is widely distributed, easy to obtain, simple to operate, and can be obtained at almost zero cost. Converting it into feed through microbial fermentation can greatly reduce feed expenses. Third, it can be beneficial to ecological protection and conform to the current concept of green agriculture and sustainable development.

[0003] However, the quality of feed prepared from straw fermented by microorganisms is currently affected by many factors, such as the characteristics of the strains, ambient temperature, and humidity. In particular, when the strain ratio is inappropriate, they cannot work together to achieve the best effect, and may even cause contamination by foreign bacteria, resulting in fermentation failure, mold and deterioration of the feed, and harm to livestock health. Summary of the invention

[0004] Although the microbial fermentation of straw into feed has broad prospects, the above-mentioned drawbacks also need to be solved urgently. Therefore, the present invention provides a special microbial flora preparation for preparing feed by straw fermentation with low cost and high efficiency, which is particularly suitable for wheat straw micro-storage feed after adjustment and optimization, and a method for preparing wheat straw fermentation feed is established, which can effectively solve the above-mentioned problems, can empower the transformation and upgrading of animal husbandry, and has important application value.

[0005] The straw feed microbial agent of the invention comprises Phanerochaete chrysosporium, Lactobacillus reuteri, Lactobacillus paracasei, Bacillus subtilis and Bacillus cereus.

[0006] The Phanerodontiachrysosporium is Phanerodontiachrysosporium SX-7-2, which is deposited in the General Microbiological Center of the China Microbiological Culture Collection Administration - CGMCC, and is classified and named Phanerodontiachrysosporium. The deposit number is CGMCC No.41406, and the deposit date is July 9, 2024.

[0007] The Lactobacillus reuteri is Lactobacillus reuteri LR301, which is deposited in the General Microbiology Center of China Microorganism Culture Collection Administration-CGMCC, classified and named Lactobacillus reuteri, with a deposit number of CGMCC No.29769 and a deposit date of January 25, 2024.

[0008] Lactobacillus paracasei, Bacillus subtilis and Bacillus cereus were commercially purchased.

[0009] The straw feed microbial agent of the present invention comprises the following components by volume:

[0010] 10-30 parts of Phanerochaete chrysosporium fermentation liquid, 20-30 parts of Lactobacillus reuteri fermentation liquid, 10-30 parts of Lactobacillus paracasei fermentation liquid, 20-30 parts of Bacillus subtilis fermentation liquid, and 10-30 parts of Bacillus cereus fermentation liquid.

[0011] The number of viable bacteria in the straw feed microbial agent of the present invention is 1×10 7 Up to 1×10 10 In a specific embodiment, the number of viable bacteria CFU of the strain in the microbial composite flora preparation used is 1×10 8 .

[0012] The straw feed microbial agent is prepared by the following method:

[0013] The fungal fermentation system is used to ferment Phanerochaete chrysosporium; the lactic acid bacteria liquid fermentation system is used to ferment Lactobacillus reuteri and Lactobacillus paracasei respectively; the Bacillus liquid fermentation system is used to ferment Bacillus subtilis and Bacillus cereus respectively, and after obtaining the fermentation liquid of each strain, they are mixed and compounded in proportion to obtain a microbial composite flora preparation.

[0014] Specific:

[0015] The fermentation liquid of Phanerochaete chrysosporium is obtained by the following method: using YPD liquid fermentation medium, the temperature is 20-30°C, the pH is 4.0-7.0, the rotation speed is 80-150 rpm, the fermentation is carried out for 24-96 hours, and the number of live bacteria of Phanerochaete chrysosporium CFU is (1.0-3.0)×10 10 .

[0016] The Lactobacillus reuteri fermentation liquid is obtained by the following fermentation method: using MRS liquid fermentation medium, the temperature is 30-37°C, the pH is 4.0-6.0, the rotation speed is 80-150 rpm, the fermentation is carried out for 48-96 hours, and the number of live bacteria CFU of Lactobacillus reuteri is (5.0-6.0)×10 10 .

[0017] The Lactobacillus paracasei fermentation liquid is obtained by the following fermentation method: using MRS liquid fermentation medium, the temperature is 30-37°C, the pH is 4.0-6.0, the rotation speed is 80-150 rpm, the fermentation is carried out for 48-96 hours, and the viable bacterial count CFU of Lactobacillus paracasei is (5.0-6.0)×10 10 .

[0018] The Bacillus subtilis fermentation liquid is obtained by fermentation in the following manner: using LB liquid fermentation medium, at a temperature of 28-40°C, a pH of 5.0-6.0, a rotation speed of 100-220 rpm, fermenting for 24-48 hours, and the number of viable Bacillus subtilis CFU is (6.0-7.0)×10 10 .

[0019] The Bacillus cereus fermentation liquid is obtained by fermentation in the following method: using LB liquid fermentation medium, the temperature is 28-40°C, the pH is 5.0-6.0, the rotation speed is 100-220rpm, the fermentation is carried out for 24-48 hours, and the number of live bacteria CFU of Bacillus cereus is (6.0-7.0)×10 10 .

[0020] The straw feed microbial agent of the present invention also includes application in preparing feed by straw fermentation.

[0021] Furthermore, the straw is crushed into a length of 1-3 cm, the liquid bacterial agent is evenly sprayed on the straw, and the straw is put into a fermentation bag, which is evacuated and sealed. After the fermentation is completed for 20-40 days, the physical and chemical properties of the wheat straw feed are tested.

[0022] The liquid microbial agent is prepared by adding water to the straw feed microbial agent.

[0023] The straw is preferably wheat straw.

[0024] Based on 100 parts by mass of straw, the inoculation amount of the straw feed microbial agent is 0.1-0.5 parts, and the amount of water added when preparing the liquid agent is 40-70 parts.

[0025] The straw feed microbial inoculant of the present invention has a good fermentation effect on the preparation of feed by straw micro-storage, and is particularly suitable for the fermentation preparation of wheat straw feed.

[0026] Compared with the prior art, the beneficial effects of the present invention are as follows:

[0027] The invention optimizes the fermentation conditions of Phanerochaete chrysosporium, Lactobacillus reuteri, Lactobacillus paracasei, Bacillus subtilis and Bacillus cereus in the microbial preparation, including temperature, rotation speed, time and the like, and finally establishes a high-density fermentation system, thereby reducing production costs. The above strains are mixed in proportion to prepare a wheat straw micro-storage composite microbial microbial preparation, and the number of live bacteria in the microbial preparation is not lower than the commercialization requirement.

[0028] The invention optimizes the composition of Phanerochaete chrysosporium, Lactobacillus reuteri, Lactobacillus paracasei, Bacillus subtilis and Bacillus cereus in a bacterial flora preparation. The obtained wheat straw micro-storage agent is used in fermenting wheat to prepare feed. Compared with conventional straw silage feed, the crude protein content is increased by 28.79%, the acetic acid content is increased by 42.67%, the propionic acid content is increased by 28.31%, the lactic acid content is increased by 103.15%, the phosphorus content is increased by 33.19%, and the calcium content is increased by 34.30%. The palatability and nutritional value of the straw feed are significantly improved, and the invention has the advantages of low cost, simple operation, shortened wheat straw feed preparation cycle, and the like. DETAILED DESCRIPTION

[0029] The terms used in the present invention, unless otherwise specified, generally have the meanings commonly understood by those of ordinary skill in the art. In the following examples, various processes and methods not described in detail are conventional methods known in the art. The specific embodiments of the present invention described below are to supplement the purpose, technical solutions and effects of the present invention, but they are not intended to limit the scope of protection of the present invention. Within the scope of the present invention, simple changes, modifications or replacements can be made, which are all considered to be within the scope of protection of the present invention.

[0030] Among the straw feed microbial agent, the present invention provides a Phanerochaete chrysosporium SX-7-2, which is deposited in the General Microbiological Center of China National Microbiological Culture Collection Committee-CGMCC, with a deposit number of CGMCC No.41460 and a deposit date of July 9, 2024.

[0031] Among the straw feed microbial agent, the Lactobacillus reuteri LR301 provided by the present invention is deposited in the General Microbiological Center of China National Microbiological Culture Collection Committee-CGMCC, with a deposit number of CGMCC No.29769 and a deposit date of January 25, 2024.

[0032] Among the straw feed microbial inoculants, the Lactobacillus paracasei, Bacillus subtilis and Bacillus cereus used in the present invention are purchased through conventional market channels.

[0033] The culture medium used in the present invention is:

[0034] YPD liquid fermentation medium: 10 g yeast extract, 20 g peptone, 20 g glucose, distilled water to 1 L, sterilized at 115°C for 30 min.

[0035] LB liquid fermentation medium: peptone 10 g, yeast extract powder 5 g, NaCl 10 g, distilled water to 1 L, sterilize at 115 °C for 30 min.

[0036] MRS liquid fermentation medium: 10 g of peptone, 5 g of beef powder, 4 g of yeast extract powder, 2 g of glucose, 801 mL of Tween, 2 g of dipotassium hydrogen phosphate, 5 g of sodium acetate, 2 g of triammonium citrate, 0.2 g of magnesium sulfate, 0.05 g of manganese sulfate, dilute to 1 L with distilled water, and sterilize at 115°C for 30 min.

[0037] Example 1: Fermentation preparation of straw microflora preparation

[0038] Based on the initial culture conditions of fungi, lactic acid bacteria and spore-forming fungi, the fermentation preparation conditions of the strains, such as temperature (25°C, 30°C, 37°C, 40°C), pH (4.0, 4.5, 5.0, 5.5, 6.0, 6.5, 7.0), etc., were optimized, and the number of live cells in the fermentation liquid was used as the evaluation standard to obtain the optimal fermentation conditions of the strains.

[0039] The culture medium of Phanerochaete chrysosporium was YPD, at 28°C, pH 6.0, and 120 rpm for 48 h. The CFU counts were 1.7×10 10 .

[0040] Lactobacillus reuteri and Lactobacillus paracasei were fermented in MRS medium at 37°C, pH 5.5, and 100 rpm for 72 h. At this time, the CFU counts of Lactobacillus reuteri and Lactobacillus paracasei were 5.1×10 10 , 5.3×10 10 .

[0041] Bacillus subtilis and Bacillus cereus were fermented in LB medium at 37°C, pH 7.0, and 200 rpm for 48 h. The CFU counts of Bacillus subtilis and Bacillus cereus were 6.2×10 10 , 6.1×10 10 .

[0042] Example 2: Application of composite microbial inoculants in preparing feed using wheat straw micro-storage

[0043] After preparing Phanerochaete chrysosporium SX-7-2, Lactobacillus reuteri LR301, Lactobacillus paracasei, Bacillus subtilis and Bacillus cereus under the above optimal fermentation conditions, the bacterial fermentation liquid is obtained. The wheat straw is crushed to 2 cm in length.

[0044] In one embodiment A:

[0045] 100 portions of Lactobacillus reuteri LR301 fermentation broth were used to prepare wheat straw micro-storage inoculants. The CFU of the live bacteria in the inoculants was 1×10 8 .

[0046] Take 100 parts of crushed wheat straw, take 0.1 parts of the above-mentioned wheat straw micro-storage agent, add it to 50 parts of water and mix evenly, spray the mixture on the wheat straw, put it into a fermentation bag, extract the air and compact it, ferment it for 30 days, and after the end, detect the changes in the crude protein, acetic acid, propionic acid, lactic acid, phosphorus and calcium content of the wheat straw.

[0047] In one embodiment B:

[0048] 100 portions of Lactobacillus paracasei fermentation broth were used to prepare wheat straw micro-storage inoculants. The number of viable bacteria in the inoculant was 1×10 8 .

[0049] Take 100 parts of crushed wheat straw, take 0.1 parts of the above-mentioned wheat straw micro-storage agent, add it to 50 parts of water and mix evenly, spray the mixture on the wheat straw, put it into a fermentation bag, extract the air and compact it, ferment it for 30 days, and after the end, detect the changes in the crude protein, acetic acid, propionic acid, lactic acid, phosphorus and calcium content of the wheat straw.

[0050] In one embodiment C:

[0051] 50 parts of Lactobacillus reuteri LR301 fermentation liquid and 50 parts of Lactobacillus paracasei fermentation liquid were mixed to prepare wheat straw micro-storage inoculant. The number of viable bacteria CFU in the inoculant was 1×10 8 .

[0052] Take 100 parts of crushed wheat straw, take 0.1 parts of the above-mentioned wheat straw micro-storage agent, add it to 50 parts of water and mix evenly, spray the mixture on the wheat straw, put it into a fermentation bag, extract the air and compact it, ferment it for 30 days, and after the end, detect the changes in the crude protein, acetic acid, propionic acid, lactic acid, phosphorus and calcium content of the wheat straw.

[0053] In one embodiment D:

[0054] 50 parts of Lactobacillus paracasei fermentation liquid and 50 parts of Phanerochaete chrysosporium SX-7-2 fermentation liquid were mixed to prepare wheat straw micro-storage inoculant. The CFU of the live bacteria in the inoculant was 1×10 8 .

[0055] Take 100 parts of crushed wheat straw, take 0.1 parts of the above-mentioned wheat straw micro-storage agent, add it to 50 parts of water and mix evenly, spray the mixture on the wheat straw, put it into a fermentation bag, extract the air and compact it, ferment it for 30 days, and after the end, detect the changes in the crude protein, acetic acid, propionic acid, lactic acid, phosphorus and calcium content of the wheat straw.

[0056] In one embodiment E:

[0057] 50 parts of Lactobacillus paracasei fermentation liquid and 50 parts of Bacillus cereus fermentation liquid were mixed to prepare wheat straw micro-storage inoculant. The number of viable bacteria CFU in the inoculant was 1×10 8 .

[0058] Take 100 parts of crushed wheat straw, take 0.1 parts of the above-mentioned wheat straw micro-storage agent, add it to 50 parts of water and mix evenly, spray the mixture on the wheat straw, put it into a fermentation bag, extract the air and compact it, ferment it for 30 days, and after the end, detect the changes in the crude protein, acetic acid, propionic acid, lactic acid, phosphorus and calcium content of the wheat straw.

[0059] In one embodiment F:

[0060] 50 parts of Lactobacillus paracasei fermentation liquid and 50 parts of Bacillus subtilis fermentation liquid were mixed to prepare wheat straw micro-storage inoculant. The number of viable bacteria CFU in the inoculant was 1×10 8 .

[0061] Take 100 parts of crushed wheat straw, take 0.1 parts of the above-mentioned wheat straw micro-storage agent, add it to 50 parts of water and mix evenly, spray the mixture on the wheat straw, put it into a fermentation bag, extract the air and compact it, ferment it for 30 days, and after the end, detect the changes in the crude protein, acetic acid, propionic acid, lactic acid, phosphorus and calcium content of the wheat straw.

[0062] In one embodiment G:

[0063] 30 parts of Lactobacillus paracasei fermentation broth, 50 parts of Phanerochaete chrysosporium SX-7-2 fermentation broth, and 20 parts of Bacillus subtilis fermentation broth were mixed to prepare wheat straw micro-storage inoculant. The CFU of the live bacteria in the inoculant was 1×10 8 .

[0064] Take 100 parts of crushed wheat straw, take 0.1 parts of the above-mentioned wheat straw micro-storage agent, add it to 50 parts of water and mix evenly, spray the mixture on the wheat straw, put it into a fermentation bag, extract the air and compact it, ferment it for 30 days, and after the end, detect the changes in the crude protein, acetic acid, propionic acid, lactic acid, phosphorus and calcium content of the wheat straw.

[0065] In one embodiment H:

[0066] 30 parts of Lactobacillus paracasei fermentation liquid, 50 parts of Phanerochaete chrysosporium SX-7-2 fermentation liquid, and 20 parts of Bacillus cereus fermentation liquid were mixed to prepare wheat straw micro-storage inoculant. The number of viable bacteria CFU in the inoculant was 1×10 8 .

[0067] Take 100 parts of crushed wheat straw, take 0.1 parts of the above-mentioned wheat straw micro-storage agent, add it to 50 parts of water and mix evenly, spray the mixture on the wheat straw, put it into a fermentation bag, extract the air and compact it, ferment it for 30 days, and after the end, detect the changes in the crude protein, acetic acid, propionic acid, lactic acid, phosphorus and calcium content of the wheat straw.

[0068] In one embodiment 1:

[0069] 30 parts of Lactobacillus paracasei fermentation liquid, 40 parts of Bacillus subtilis fermentation liquid, and 30 parts of Bacillus cereus fermentation liquid were mixed to prepare wheat straw micro-storage inoculant. The number of viable bacteria CFU in the inoculant was 1×10 8 .

[0070] Take 100 parts of crushed wheat straw, take 0.1 parts of the above-mentioned wheat straw micro-storage agent, add it to 50 parts of water and mix evenly, spray the mixture on the wheat straw, put it into a fermentation bag, extract the air and compact it, ferment it for 30 days, and after the end, detect the changes in the crude protein, acetic acid, propionic acid, lactic acid, phosphorus and calcium content of the wheat straw.

[0071] In one embodiment J:

[0072] 30 parts of fermentation broth of Phanerochaete chrysosporium SX-7-2, 20 parts of fermentation broth of Lactobacillus reuteri LR301, 20 parts of fermentation broth of Lactobacillus paracasei, 20 parts of fermentation broth of Bacillus subtilis, and 10 parts of fermentation broth of Bacillus cereus were mixed to prepare wheat straw micro-storage inoculant. The number of viable bacteria CFU in the inoculant was 1×10 8 .

[0073] Take 100 parts of crushed wheat straw, take 0.1 parts of the above-mentioned wheat straw micro-storage agent, add it to 50 parts of water and mix evenly, spray the mixture on the wheat straw, put it into a fermentation bag, extract the air and compact it, ferment it for 30 days, and after the end, detect the changes in the crude protein, acetic acid, propionic acid, lactic acid, phosphorus and calcium content of the wheat straw.

[0074] In one embodiment K:

[0075] 10 parts of fermentation liquid of Phanerochaete chrysosporium SX-7-2, 25 parts of fermentation liquid of Lactobacillus reuteri LR301, 10 parts of fermentation liquid of Lactobacillus paracasei, 25 parts of fermentation liquid of Bacillus subtilis, and 30 parts of fermentation liquid of Bacillus cereus were mixed to prepare wheat straw micro-storage inoculant. The number of viable bacteria CFU of the strains in the inoculant was 1×10 8 .

[0076] Take 100 parts of crushed wheat straw, take 0.1 parts of the above-mentioned wheat straw micro-storage agent, add it to 50 parts of water and mix evenly, spray the mixture on the wheat straw, put it into a fermentation bag, extract the air and compact it, ferment it for 30 days, and after the end, detect the changes in the crude protein, acetic acid, propionic acid, lactic acid, phosphorus and calcium content of the wheat straw.

[0077] In one embodiment L:

[0078] 15 parts of fermentation broth of Phanerochaete chrysosporium SX-7-2, 30 parts of fermentation broth of Lactobacillus reuteri LR301, 15 parts of fermentation broth of Lactobacillus paracasei, 30 parts of fermentation broth of Bacillus subtilis, and 10 parts of fermentation broth of Bacillus cereus were mixed to prepare wheat straw micro-storage inoculant. The CFU of the live bacteria in the inoculant was 1×10 8 .

[0079] Take 100 parts of crushed wheat straw, take 0.1 parts of the above-mentioned wheat straw micro-storage agent, add it to 50 parts of water and mix evenly, spray the mixture on the wheat straw, put it into a fermentation bag, extract the air and compact it, ferment it for 30 days, and after the end, detect the changes in the crude protein, acetic acid, propionic acid, lactic acid, phosphorus and calcium content of the wheat straw.

[0080] In one embodiment M:

[0081] 10 parts of fermentation liquid of Phanerochaete chrysosporium SX-7-2, 20 parts of fermentation liquid of Lactobacillus reuteri LR301, 30 parts of fermentation liquid of Lactobacillus paracasei, 30 parts of fermentation liquid of Bacillus subtilis, and 10 parts of fermentation liquid of Bacillus cereus were mixed to prepare wheat straw micro-storage inoculant. The number of viable bacteria CFU of the strains in the inoculant was 1×10 8 .

[0082] Take 100 parts of crushed wheat straw, take 0.1 parts of the above-mentioned wheat straw micro-storage agent, add it to 50 parts of water and mix evenly, spray the mixture on the wheat straw, put it into a fermentation bag, extract the air and compact it, ferment it for 30 days, and after the end, detect the changes in the crude protein, acetic acid, propionic acid, lactic acid, phosphorus and calcium content of the wheat straw.

[0083] The same wheat straw fermentation treatment as mentioned above was used as the control group, except that an equal amount of sterile water was added instead of the microbial compound agent.

[0084] The test results are shown in Table 1. The microbial agents have different degrees of improvement and influence on the physical and chemical properties of wheat straw micro-silo feed products. Among them, the comprehensive evaluation of Example 1 considering the fermentation preparation of wheat straw feed has the best effect. Compared with conventional straw yellow silo feed, the crude protein content is increased by 28.79%, the acetic acid content is increased by 42.67%, the propionic acid content is increased by 28.31%, the lactic acid content is increased by 103.15%, the phosphorus content is increased by 33.19%, and the calcium content is increased by 34.30%, which significantly improves the palatability and nutritional value of the straw feed, and has the advantages of low cost, simple operation, and shortened wheat straw feed preparation cycle.

[0085]

Claims

1. A straw feed microbial agent, characterized in that: The straw feed microbial inoculant includes Phanerochaete chrysosporium, Lactobacillus reuteri, Lactobacillus paracasei, Bacillus subtilis and Bacillus cereus; The Phanerodontia chrysosporium is Phanerodontia chrysosporium SX-7-2, which is deposited in the General Microbiological Center of China Microbiological Culture Collection Administration-CGMCC, and is classified and named Phanerodontia chrysosporium, with a deposit number of CGMCC No.41406 and a deposit date of July 9, 2024; The Lactobacillus reuteri is Lactobacillus reuteri LR301, which is deposited in the General Microbiology Center of China Microorganism Culture Collection Administration-CGMCC, classified and named Lactobacillus reuteri, with a deposit number of CGMCC No.29769 and a deposit date of January 25, 2024.

2. The straw feed microbial agent according to claim 1, characterized in that The components by volume are as follows: 10-30 parts of Phanerochaete chrysosporium fermentation liquid, 20-30 parts of Lactobacillus reuteri fermentation liquid, 10-30 parts of Lactobacillus paracasei fermentation liquid, 20-30 parts of Bacillus subtilis fermentation liquid, and 10-30 parts of Bacillus cereus fermentation liquid.

3. The straw feed microbial agent according to claim 2, characterized in that: The number of viable bacteria in the straw feed microbial agent is 1×10 7 Up to 1×10 10 .

4. The straw feed microbial agent according to claim 2, characterized in that: Using a fungal fermentation system, Phanerochaete chrysosporium was fermented; using a lactic acid bacteria liquid fermentation system, Lactobacillus reuteri and Lactobacillus paracasei were fermented respectively; Bacillus subtilis and Bacillus cereus are fermented separately in a Bacillus liquid fermentation system, and after obtaining the fermentation liquid of each strain, they are mixed and compounded in proportion to obtain a microbial composite flora preparation.

5. Use of the straw feed microbial agent according to any one of claims 1 to 4 in preparing feed by fermenting wheat straw.

6. The use of the straw feed microbial agent according to claim 5 in preparing feed by fermentation of wheat straw, characterized in that: Crush the wheat straw into 1-3 cm length, spray the liquid bacterial agent evenly on the wheat straw, put it into the fermentation bag, evacuate and seal it, and after fermentation for 20-40 days, test the physical and chemical properties of the wheat straw feed; The liquid microbial agent is prepared by adding water to the straw feed microbial agent.

7. The use of the straw feed microbial agent according to claim 6 in preparing feed by fermentation of wheat straw, characterized in that: Based on 100 parts by mass of wheat straw, the inoculation amount of the straw feed microbial agent is 0.1-0.5 parts, and the amount of water added when preparing the liquid agent is 40-70 parts.

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