A method, device and apparatus for dispensing cell suspension

Abstract

The application discloses a cell liquid sub-packaging method, device and equipment. The cell liquid sub-packaging method comprises the following steps: obtaining a mixing capacity of a mixing bag and a mixing cell type; after receiving a pipeline consumable loading instruction, obtaining a sub-packaging cell type and a sub-packaging capacity on each sub-packaging bag; performing type confirmation on the mixing cell type and the sub-packaging cell type to obtain a type confirmation result corresponding to each sub-packaging bag, and performing capacity confirmation on the mixing capacity and the sub-packaging capacity to obtain a capacity confirmation result corresponding to each sub-packaging bag; after receiving a sub-packaging sequence, adaptively generating a liquid path diagram according to the sub-packaging sequence, the obtained sub-packaging cell type and the obtained sub-packaging capacity; the sub-packaging sequence is a sequence set for the sub-packaging bags with qualified capacity confirmation results and type confirmation results; and sub-packaging the mixed liquid in the mixing bag into target sub-packaging bags according to the liquid path diagram. The sub-packaging method can accurately sub-packaging without setting a sub-packaging sequence.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of cell dispensing, in particular to a cell liquid dispensing method, device and equipment. BACKGROUND

[0002] In the field of cell therapy, the traditional cell dispensing has set a fixed sequence to accurately dispense; and the existing dispensing process records the information of the dispensing bag manually, cannot realize the information transmission and traceability of the dispensing bag, cannot solve the problems of random sequence dispensing, dispensing source traceability and process inspection, etc., so that the operator needs to spend effort to record, which is not only time-consuming and error-prone, but also low in efficiency. SUMMARY

[0003] The present application provides a cell liquid dispensing method, device and equipment, which adopts the sequence set by the dispensing bag with qualified capacity confirmation result and type confirmation result as the dispensing sequence for automatic dispensing, and can accurately dispense without setting the dispensing sequence; and combines the identification code on the dispensing bag to achieve flexible traceability of the dispensing bag.

[0004] The present application adopts the following specific technical solutions:

[0005] A cell liquid dispensing method, which comprises the following steps:

[0006] Obtaining the mixing capacity and mixing cell type of the mixing bag;

[0007] After receiving the pipeline consumable loading instruction, obtaining the dispensing cell type and dispensing capacity on each dispensing bag;

[0008] Performing type confirmation on the mixing cell type and each dispensing cell type to obtain the type confirmation result corresponding to each dispensing bag, and performing capacity confirmation on the mixing capacity and each dispensing capacity to obtain the capacity confirmation result corresponding to each dispensing bag;

[0009] After receiving the dispensing sequence, generating a liquid path map adaptively according to the dispensing sequence and the obtained dispensing cell type and dispensing capacity; the dispensing sequence is the sequence set by the dispensing bag with qualified capacity confirmation result and type confirmation result;

[0010] According to the liquid path map, dispensing the mixed liquid in the mixing bag into the target dispensing bag.

[0011] Further, the obtaining of the mixing capacity and mixing cell type of the mixing bag comprises:

[0012] Receiving the mixing cell type after selection confirmation;

[0013] Detecting the flow rate of the mixed cells flowing into the mixed bag by a sensor to obtain the mixed volume.

[0014] Further, the volume confirmation of the mixed volume and each of the aliquot volumes obtains a volume confirmation result corresponding to each aliquot bag, including:

[0015] Summing up the aliquot volumes corresponding to all aliquot cell types of the same mixed cell type to obtain an aliquot total;

[0016] Judging whether the aliquot total is within the mixed volume range;

[0017] If it is within the mixed volume range, the volume confirmation result is qualified;

[0018] If it is not within the mixed volume range, the volume confirmation result is unqualified, and the aliquot volume needs to be corrected.

[0019] Further, the liquid path map is adaptively generated according to the aliquot sequence, and each aliquot cell type and each aliquot volume obtained, including:

[0020] According to the starting liquid bag, the jump start liquid bag, the jump end liquid bag and the ending liquid bag in the aliquot sequence, the liquid path map matching them is found out in the historical liquid path map set, and the found matching liquid path map is recorded as a reference liquid path map;

[0021] Difference self-learning is performed on all the reference liquid path maps to generate the liquid path map.

[0022] Further, the difference self-learning on all the reference liquid path maps to generate the liquid path map includes:

[0023] Calculating the difference degree between the historical volume in each of the reference liquid path maps and each aliquot volume to obtain a volume difference value;

[0024] Calculating the matching degree between the historical sequence in each of the reference liquid path maps and the aliquot sequence to obtain a sequence matching value;

[0025] According to the volume difference value and the sequence matching value of each of the reference liquid path maps, a most matching liquid path map is obtained;

[0026] Residual amount learning is performed on the volume difference value and the sequence matching value of the most matching liquid path map to obtain a residual amount path;

[0027] The residual amount path is integrated into the most matching liquid path map to obtain the liquid path map.

[0028] Further, the residual amount learning on the volume difference value and the sequence matching value of the most matching liquid path map to obtain a residual amount path includes:

[0029] According to the capacity difference value and the order matching value of the most matched liquid path map, a residual amount is obtained;

[0030] The residual amount is path-decomposed by pushing liquid and air extraction, and the shortest pushing liquid path and air extraction path are combined into a residual amount path.

[0031] Further, the step of distributing the mixed liquid in the mixing bag to the target distribution bag according to the liquid path map comprises:

[0032] According to the distribution capacity of each target distribution bag in the liquid path map, the distribution rotation speed of each distribution bag is correspondingly gradient-decreased according to the distribution capacity, and the mixed liquid is distributed to the target distribution bag.

[0033] Further, each distribution bag is provided with an identification code, and the distribution cell type and the distribution capacity of the corresponding distribution bag can be obtained by scanning the identification code.

[0034] Meanwhile, the application provides a cell liquid distribution device, which comprises:

[0035] The acquisition module is configured to acquire the mixed capacity and the mixed cell type of the mixing bag.

[0036] The receiving module is configured to acquire the distribution cell type and the distribution capacity of each distribution bag after receiving the pipeline consumable loading instruction.

[0037] The confirmation module is configured to confirm the types of the mixed cell type and each distribution cell type to obtain a type confirmation result corresponding to each distribution bag, and confirm the capacities of the mixed capacity and each distribution capacity to obtain a capacity confirmation result corresponding to each distribution bag.

[0038] The generation module is configured to generate a liquid path map according to the distribution sequence, the acquired distribution cell type and the acquired distribution capacity after receiving the distribution sequence; the distribution sequence is a sequence set for the distribution bag with both the capacity confirmation result and the type confirmation result being qualified.

[0039] The distribution module is configured to distribute the mixed liquid in the mixing bag to the target distribution bag according to the liquid path map.

[0040] In addition, the application further provides a cell liquid distribution device, which comprises a mixing bag, a distribution bag and a controller, and the controller is configured to execute any one of the cell liquid distribution methods provided in the above technical solutions.

[0041] Advantages:

[0042] The cell liquid sub-packaging method of the present application generates a liquid path map adaptively according to the sub-packaging sequence and the obtained sub-packaging cell type and sub-packaging capacity after receiving the sub-packaging sequence, and sub-packages the mixed liquid in the mixed bag into the target sub-packaging bag according to the liquid path map in the order of the sub-packaging bag whose capacity confirmation result and type confirmation result are both qualified, so that the sub-packaging cell type and sub-packaging capacity are accurate and correct, and the problems of batch comparison, sub-packaging source tracing and process inspection of sub-packaging are solved. At the same time, the cumbersome recording process can be automated through the identification code on the sub-packaging bag. The user can make the identification code in advance and paste it on the sub-packaging bag. When the sub-packaging bag is ready for sub-packaging, the instrument control automatically scans the device, automatically obtains the bar code and information on the sub-packaging bag, and records them together into the process record. After the sub-packaging is completed, the operator can flexibly refrigerate or process the sub-packaging bag according to his own needs, without worrying about the order of the sub-packaging bag in this sub-packaging. The user can track the state and information of each sub-packaging bag for a long time through the identification code, and solve the problems of time-consuming, error-prone and low efficiency caused by the need of the operator to record due to the problems of sub-packaging in random order, sub-packaging source tracing and process inspection. BRIEF DESCRIPTION OF DRAWINGS

[0043] Figure 1 The flow chart of the cell liquid sub-packaging method of the present application;

[0044] Figure 2 The structure diagram of the sub-packaging pipeline with upper and lower liquid outlet ports. DETAILED DESCRIPTION

[0045] The technical solutions in the embodiments of the present application will be described clearly and completely below with reference to the drawings in the embodiments of the present application. Obviously, the described embodiments are only part of the embodiments of the present application, not all the embodiments. Based on the embodiments in the present application, all other embodiments obtained by those skilled in the art without creative labor are within the scope of protection of the present application.

[0046] Unless otherwise defined, all technical terms used in the embodiments have the same meanings as commonly understood by those skilled in the art; the terms used in the specification are only for the purpose of describing the specific embodiments, not intended to limit the present application; the terms "include" and "have" and any variations thereof in the specification and claims of the present application and the above description of drawings are intended to cover non-exclusive inclusion. The terms "first", "second" and the like in the specification and claims of the present application or the above description of drawings are used to distinguish different objects, not to describe a specific order.

[0047] Embodiment one

[0048] The embodiment provides a cell liquid dispensing method, as shown in the figure, which comprises the following steps: Figure 1

[0049] The first step S1 is to obtain a mixing capacity of a mixing bag and a mixing cell type, and specifically comprises the following steps:

[0050] The mixing cell type after selection confirmation is received; the mixing capacity is obtained by detecting the flow rate of the mixing bag through a sensor;

[0051] The second step S2 is to obtain a dispensing cell type and a dispensing capacity of each dispensing bag after receiving a pipeline consumable loading instruction; each dispensing bag is provided with an identification code, and the dispensing cell type and the dispensing capacity of the corresponding dispensing bag can be obtained by scanning the identification code; the identification code can be a bar code or a two-dimensional code; before process operation, the identification code of the dispensing bag can be made according to the self needs, and the identification code can contain the cell type name, the capacity of the dispensing bag and the like, and the identification code of the dispensing control information required for dispensing can be printed by inputting the cell type name and the capacity in a bar code generation device; each dispensing bag has a unique identification code; by making a unique identification code for each dispensing bag, the dispensing sequence does not need to be input, and the dispensing sequence of the traditional dispensing needs to be set to accurately dispense; the printed identification code is pasted on the dispensing bag, so that the identification code can be scanned by an automatic code scanning device; the identification code corresponding to the bar code is pasted according to the capacity of the bag, and the bar code is the bar code of the dispensing bag itself;

[0052] The third step S3 is to perform type confirmation on the mixing cell type and each dispensing cell type to obtain a type confirmation result corresponding to each dispensing bag, and to perform capacity confirmation on the mixing capacity and each dispensing capacity to obtain a capacity confirmation result corresponding to each dispensing bag; the capacity confirmation on the mixing capacity and each dispensing capacity obtains the capacity confirmation result corresponding to each dispensing bag, and specifically comprises the following steps:

[0053] The dispensing capacities corresponding to all dispensing cell types which are the same as the mixing cell type are summed up to obtain a dispensing total; it is judged whether the dispensing total is within the mixing capacity range; if yes, the capacity confirmation result is qualified; if not, the capacity confirmation result is unqualified, and the dispensing capacity needs to be corrected;

[0054] The fourth step S4 is to adaptively generate a liquid path map according to the dispensing sequence, the dispensing cell type and the dispensing capacity obtained after receiving the dispensing sequence; the dispensing sequence is the sequence set for the dispensing bags with qualified capacity confirmation result and type confirmation result; the liquid path map is adaptively generated according to the dispensing sequence, the dispensing cell type and the dispensing capacity obtained, and specifically comprises the following steps:

[0055] ​According to the starting liquid bag, the jump starting liquid bag, the jump ending liquid bag and the ending liquid bag in the order of sub-packaging, the liquid path diagram matched with them is found out in the historical liquid path diagram set, and the found matched liquid path diagram is recorded as the reference liquid path diagram; the starting liquid bag is the liquid bag that starts sub-packaging first; the jump starting and ending liquid bags are the sub-packaging tank that does not need to be sub-packaged and the sub-packaging tank that jumps; the ending sub-packaging liquid bag is the liquid bag that is sub-packaged last; difference self-learning is performed on all reference liquid path diagrams to generate a liquid path diagram; the adaptive method is: the matching degree is calculated through the capacity dimension and the serial number dimension of the sub-packaging tank, the different parameters in the most matched liquid path diagram are self-learned, the residual amount in the pipeline is analyzed, and back-pumping and emptying are performed according to the residual amount; back-pumping, that is, according to the residual amount between the upper and lower delivery ports and the interface between the upper delivery port and the last sub-packaging bag of the upper row / the interface between the lower delivery port and the last sub-packaging bag of the lower row, the liquid is appropriately back-pumped to the lower delivery port; emptying, that is, when the liquid is about to be sub-packaged to the ending liquid bag, the time to stop liquid supply can be recognized, only the liquid in the peristaltic pump transmission pipeline is transmitted after the time, the liquid supply is not continued, and the liquid in the pipeline is emptied to the ending sub-packaging bag; the liquid path diagram defines the flow path of the liquid path; because the pipeline is branched into two rows between the upper and lower delivery ports, there is residual liquid in the pipeline between the branches of the two rows. Figure 2 The sub-packaging pipeline with the upper and lower delivery ports is shown, the upper and lower rows each have 10 delivery ports, and 20 sub-packaging bags can be sub-packaged.

[0056] The fifth step S5 is to sub-packaging the mixed liquid in the mixed bag to the target sub-packaging bag according to the liquid path diagram, specifically including:

[0057] According to the sub-packaging capacity of each target sub-packaging bag in the liquid path diagram, the sub-packaging rotation speed of each sub-packaging bag is decreased in a gradient corresponding to the sub-packaging capacity, and the mixed liquid is sub-packaged to the target sub-packaging bag. When planning the liquid path diagram, the rotation speed of the pump is controlled or the displacement of the pump in the sub-packaging equipment is switched according to the capacity of the sub-packaging bag to improve the sub-packaging accuracy; the pump can be a transmission pump driven by a stepper motor, such as a peristaltic pump.

[0058] The above cell liquid dispensing method adopts a dispensing sequence of the dispensing bag with both the volume confirmation result and the type confirmation result being qualified, and dispenses the mixed liquid in the mixed bag to the target dispensing bag according to the liquid path diagram, so that the dispensing cell type and the dispensing volume are accurate, and the problems of batch comparison, source tracing and process inspection of dispensing are solved. Meanwhile, the tedious recording process can be automated through the unique identification code on the dispensing bag. The user can make the identification code in advance and paste it on the dispensing bag. When the dispensing bag is ready for dispensing, the instrument control automatically scans the device, automatically obtains the bar code and information on the dispensing bag, and records them in the process record. After dispensing is completed, the operator can flexibly refrigerate or process the dispensing bag according to his own needs, without worrying about the sequence of the dispensing bag in this dispensing. The user can track the state and information of each dispensing bag for a long time through the identification code, and solve the problems of time-consuming, error-prone and low efficiency caused by the need of the operator to record the dispensing in a random sequence, source tracing and process inspection.

[0059] In a specific embodiment, all reference liquid path diagrams are subjected to difference self-learning to generate a liquid path diagram, specifically including the following steps:

[0060] The difference between the historical volume in each reference liquid path diagram and each dispensing volume is calculated to obtain a volume difference value; the matching degree between the historical sequence in each reference liquid path diagram and the dispensing sequence is calculated to obtain a sequence matching value; the most matching liquid path diagram is obtained according to the volume difference value and the sequence matching value of each reference liquid path diagram; the residual amount path is obtained by residual amount learning of the volume difference value and the sequence matching value of the most matching liquid path diagram; and the residual amount path is integrated into the most matching liquid path diagram to obtain a liquid path diagram.

[0061] Further, the residual amount path is obtained by residual amount learning of the volume difference value and the sequence matching value of the most matching liquid path diagram, including:

[0062] The residual amount is obtained according to the volume difference value and the sequence matching value of the most matching liquid path diagram; and the residual amount path is obtained by path decomposition of liquid pushing and air pumping of the residual amount, and the shortest liquid pushing path and air pumping path are combined into the residual amount path.

[0063] Embodiment two

[0064] Meanwhile, the application provides a cell liquid dispensing device, which realizes functions corresponding to the above cell liquid dispensing method one by one. The cell liquid dispensing device includes an acquisition module, a receiving module, a confirmation module, a generation module and a dispensing module:

[0065] The acquisition module is configured to acquire a mixing volume of the mixing bag and a mixing cell type; the receiving module is configured to acquire a dispensing cell type and a dispensing volume on each dispensing bag after receiving a pipeline consumable loading instruction; the confirmation module is configured to perform type confirmation on the mixing cell type and the dispensing cell type to obtain a type confirmation result corresponding to each dispensing bag, and perform volume confirmation on the mixing volume and the dispensing volume to obtain a volume confirmation result corresponding to each dispensing bag; the generation module is configured to adaptively generate a liquid path map according to the dispensing sequence, and the acquired dispensing cell type and dispensing volume after receiving the dispensing sequence; the dispensing sequence is a sequence set for the dispensing bag with both the volume confirmation result and the type confirmation result being qualified; and the dispensing module is configured to dispense the mixed liquid in the mixing bag into a target dispensing bag according to the liquid path map.

[0066] Embodiment three

[0067] The embodiment provides a cell liquid dispensing device, which comprises a mixing bag, a dispensing bag and a controller; and the controller is configured to execute the cell liquid dispensing method provided in the above embodiment.

[0068] Obviously, those skilled in the art can make various modifications and variations to the embodiments of the present application without departing from the spirit and scope of the present application. Thus, if these modifications and variations of the present application belong to the scope of the claims of the present application and their equivalent technologies, the present application also intends to include these modifications and variations.

Claims

1. A method for dispensing cell fluid, characterized in that, Includes the following steps: Obtain the mixing capacity and cell type of the mixing bag; After receiving the tubing consumable loading instruction, obtain the cell type and capacity of each dispensing bag; The type of the mixed cell and each of the sub-packaged cell types are confirmed to obtain the type confirmation result corresponding to each sub-package; the capacity of the mixed capacity and each of the sub-packaged capacity are confirmed to obtain the capacity confirmation result corresponding to each sub-package. Upon receiving the dispensing order, an adaptive fluid routing diagram is generated based on the dispensing order, the acquired cell types, and the dispensing capacities. This includes: identifying matching fluid routing diagrams in the historical fluid routing diagram set based on the starting fluid bag, skipped starting fluid bag, skipped ending fluid bag, and ending fluid bag in the dispensing order, and recording the matching fluid routing diagrams as reference fluid routing diagrams; and performing difference self-learning on all the reference fluid routing diagrams to generate the fluid routing diagram, including: calculating the difference between the historical capacity and the dispensing capacity in each reference fluid routing diagram. The following steps are performed: 1) Obtain the capacity difference value; 2) Calculate the matching degree between the historical order and the dispensing order in each reference liquid path diagram to obtain the order matching value; 3) Obtain the best-matching liquid path diagram based on the capacity difference value and the order matching value of each reference liquid path diagram; 4) Perform residual amount learning on the capacity difference value and the order matching value of the best-matching liquid path diagram to obtain the residual amount path; 5) Integrate the residual amount path into the best-matching liquid path diagram to obtain the liquid path diagram; 6) The dispensing order is the order set for dispensing bags where both the capacity confirmation result and the type confirmation result are qualified. According to the liquid path diagram, the mixed liquid in the mixing bag is dispensed into the target dispensing bag.

2. The cell fluid dispensing method as described in claim 1, characterized in that, The process of obtaining the mixing capacity and mixing cell type of the mixing bag includes: Receive the selected and confirmed cell type after mixing. The mixing capacity is obtained by detecting the flow rate into the mixing bag using a sensor.

3. The cell fluid dispensing method as described in claim 1, characterized in that, The process of confirming the mixing capacity and the dispensing capacity of each bag to obtain the capacity confirmation result for each dispensing bag includes: Sum the dispensing capacities corresponding to all dispensing cell types that are the same as the mixed cell types to obtain the total dispensing capacity; Determine whether the total amount of the dispensed products is within the range of the mixing capacity; If the mixing capacity is within the specified range, the capacity confirmation result is qualified; If the mixing capacity is not within the specified range, the capacity confirmation result is unqualified and the repackaging capacity needs to be corrected.

4. The cell fluid dispensing method as described in claim 1, characterized in that, The process of learning the residual amount from the capacity difference value and sequence matching value of the best-matched fluid flow diagram to obtain the residual amount path includes: The residual amount is obtained based on the capacity difference value and sequence matching value of the best matching fluid flow diagram; The residual amount is decomposed into liquid pushing and air extraction paths, and the shortest liquid pushing and air extraction paths are combined into the residual amount path.

5. The cell fluid dispensing method as described in claim 1, characterized in that, The step of dispensing the mixed liquid from the mixing bag into the target dispensing bag according to the liquid path diagram includes: According to the dispensing capacity of each target dispensing bag in the liquid path diagram, the dispensing speed of each dispensing bag is gradually decreased in a gradient corresponding to the dispensing capacity, so as to complete the dispensing of the mixed liquid into the target dispensing bag.

6. The cell fluid dispensing method according to any one of claims 1-5, characterized in that, Each of the aforementioned repackaging bags is equipped with an identification code, which can be scanned to obtain the cell type and repackaging capacity of the corresponding repackaging bag.

7. A cell fluid dispensing apparatus employing the cell fluid dispensing method according to any one of claims 1-6, characterized in that, include: The acquisition module is used to acquire the mixing capacity and cell type of the mixing bag; The receiving module is used to obtain the cell type and dispensing capacity on each dispensing bag after receiving the pipeline consumable loading instruction; The confirmation module is used to confirm the type of the mixed cell and each of the dispensed cell types, and to obtain the type confirmation result corresponding to each dispensed bag; and to confirm the capacity of the mixed capacity and each of the dispensed capacities, and to obtain the capacity confirmation result corresponding to each dispensed bag. The generation module is used to adaptively generate a liquid path diagram based on the dispensing order, the obtained cell types and capacities of each dispensing after receiving the dispensing order; the dispensing order is the order set for dispensing bags whose capacity and type confirmation results are both qualified. The dispensing module is used to dispense the mixed liquid in the mixing bag into the target dispensing bag according to the liquid path diagram.

8. A cell fluid dispensing device, characterized in that, The method includes a mixing bag, a dispensing bag, and a controller, the controller being used to perform the cell fluid dispensing method as described in any one of claims 1 to 6.

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