Organic fertilizer suitable for tea yield increase and insect killing and preparation method thereof

Abstract

The application discloses an organic fertilizer suitable for tea yield increase and insect killing and a preparation method thereof, and relates to the technical field of organic fertilizer and microbial fertilizer manufacturing. The organic fertilizer comprises straw, chicken manure, Tibetan wood incense, worry grass, mouse grass, black bone vine, tannic acid-rice protein enzymatic hydrolysate, sodium laurate modified diatomite, sodium humate, urea, compound microbial bacteria and water. The compound microbial bacteria are composed of Candida utilis and intercalary neura. The compound microbial bacteria in the organic fertilizer are helpful to accelerating the fermentation and decomposition of the straw, chicken manure, sodium humate, urea and traditional Chinese medicine plants, promoting the conversion of macromolecular substances into small-molecule active ingredients, and then assisting with the tannic acid-rice protein enzymatic hydrolysate and sodium laurate modified diatomite, so that the yield of tea tree planting is improved through reasonable proportioning of nutritional ingredients.

Description

Technical Field

[0001] This invention relates to the field of organic fertilizer and microbial fertilizer manufacturing technology, and in particular to an organic fertilizer suitable for increasing tea yield and killing pests, and its preparation method. Background Technology

[0002] Currently, many types of fertilizers are used in tea cultivation. Most tea-specific fertilizers are commercially available NPK compound fertilizers, primarily composed of the three inorganic elements N, P, and K. While these can meet the growth needs of tea trees in the short term, long-term use can damage the soil's aggregate structure, leading to soil compaction, hardening, and reduced insecticidal effects, thus impacting tea tree growth and causing a decline in tea quality and yield. Therefore, there is an urgent need to develop an organic fertilizer suitable for increasing tea production and controlling pests. Summary of the Invention

[0003] In view of the above-mentioned deficiencies in the prior art, the present invention proposes an organic fertilizer suitable for increasing tea yield and controlling pests, and its preparation method, so as to solve the problems mentioned in the background art.

[0004] To achieve the above objectives, the present invention provides the following technical solution:

[0005] An organic fertilizer suitable for increasing tea production and controlling pests comprises the following raw materials in parts by weight: 50-70 parts straw, 30-40 parts chicken manure, 15-25 parts *Inula racemosa* Hook.f. and *Inula helenium* L. (Asteraceae), 15-20 parts *Clinacanthus nutans* (Burm.F.) Lindau (Acanthaceae), 12-22 parts *Gnaphalium affine* D. Don (Asteraceae), and 10-18 parts *Periploca forrestii* (Asteraceae). (roots or whole plant of Schltr.), 8-15 parts ellagic acid-rice protein hydrolysate, 5-10 parts sodium lauryl diatomaceous earth modified with sodium lauryl lactone, 5-10 parts sodium humate, 4-15 parts urea, 1-6 parts compound microbial bacteria and 200-300 parts water.

[0006] The compound microbial strain is composed of Candida utilis and Neurospora intercala in a mass ratio of (1-5):(2-7).

[0007] Preferably, an organic fertilizer suitable for increasing tea production and controlling pests comprises the following raw materials in parts by weight: 60 parts straw, 35 parts chicken manure, 20 parts Tibetan incense, 18 parts Clematis armandii, 15 parts Gnaphalium affine, 12 parts Caulis Gnaphalium affine, 12 parts Vitis thunbergii, 12 parts ellagic acid-rice protein hydrolysate, 8 parts sodium laurate-modified diatomaceous earth, 7 parts sodium humate, 6 parts urea, 5 parts compound microbial bacteria, and 280 parts water;

[0008] The compound microbial strain is composed of Candida utilis and Neurospora intercalans in a mass ratio of 2:5.

[0009] Preferably, the method for culturing Candida utilis involves fully dissolving the purchased freeze-dried Candida utilis powder in sterile water, spreading it evenly on a plate, and then culturing it in a culture medium (yeast extract: 3g, malt extract: 3g, glucose: 10g, casein peptone: 5g, agar: 20g) under the following conditions: pH: 6.2±0.2, temperature: 25℃.

[0010] Preferably, the method for culturing Neurospora intercalans is to fully dissolve the purchased Neurospora intercalans lyophilized powder in sterile water, spread it evenly on a plate, and place it in a culture medium (potato extract powder: 10g, glucose: 20g, KH2PO4: 3g, MgSO4·7H2O: 1.5g, thiamine: 0.008g, agar: 15g) for culture under the following conditions: pH: 6.0±0.2, temperature: 25℃.

[0011] Preferably, the preparation method of the ellagic acid-rice protein hydrolysate includes the following steps:

[0012] (1) Crush rice, add water and preheat to 40-50℃, adjust pH to 9-11, add alkaline protease and hydrolyze for 200-300 min. After hydrolysis, adjust pH to 8.5-9.6 to inactivate the enzyme and obtain hydrolysate.

[0013] (2) Dissolve ellagic acid in anhydrous ethanol to obtain ellagic acid solution;

[0014] (3) Mix the enzymatic hydrolysate obtained in step (1) and the ellagic acid solution obtained in step (2), and stir for 60 to 120 minutes to obtain ellagic acid-rice enzymatic hydrolysate.

[0015] Preferably, the alkaline protease has an enzyme activity of 100,000–200,000 U / g, is a brown powder, CAS No.: 9014-01-1, and was purchased from Chengdu Wanxiang Hongrun Biotechnology Co., Ltd.

[0016] Preferably, in step (1), the ratio of rice to water is (20-30) g: 100 mL.

[0017] Preferably, in step (2), the ratio of ellagic acid to anhydrous ethanol is 1 g: (10-20) mL.

[0018] Preferably, the preparation method of the sodium lauryl diatomite modified with sodium lauryl lactone includes the following steps:

[0019] (a) Add diatomaceous earth to a 10-15 wt% sodium hydroxide aqueous solution and soak for 15-40 min, then filter to obtain pretreated diatomaceous earth;

[0020] (b) Add sodium lauryl ester and water to the pretreated diatomite obtained in step (a), stir for 10-15 minutes, cool and dry, and then grind to obtain sodium lauryl ester modified diatomite.

[0021] Preferably, in step (a), the ratio of diatomaceous earth to sodium hydroxide aqueous solution is 1 g: (3-7) mL.

[0022] Preferably, in step (b), the ratio of pretreated diatomaceous earth, sodium lauryl laurate, and water is 1g:(1.5-3)mL:(20-50)mL.

[0023] Preferably, a method for preparing an organic fertilizer suitable for increasing tea yield and controlling pests includes the following steps:

[0024] S1. Weigh the raw materials according to the stated weight proportions;

[0025] S2. First, crush the Tibetan costus root, Clinacanthus nutans, Gnaphalium affine, and black bone vine into powder, then add sodium humate and water to mix, soak for 100-200 minutes, then add urea and mix evenly to obtain the fermentation product.

[0026] S3. Add straw, chicken manure and compound microbial bacteria to the fermentable material obtained in step S2, carry out aerobic fermentation at a temperature of 25-28℃ for 10-20 days, and dry until the moisture content is less than 12-20wt% to obtain the fermentation product.

[0027] S4. Add ellagic acid-rice protein hydrolysate and sodium lauryl diatomaceous earth modified to the fermentation product obtained in step S3, mix evenly, and granulate to obtain organic fertilizer.

[0028] Preferably, a method for applying an organic fertilizer suitable for increasing tea yield and controlling pests is provided, wherein the organic fertilizer is applied using one or more of the following methods: hole application, furrow application, and broadcast application. The application rate is 40-60 kg per acre.

[0029] Preferably, the organic fertilizer involved in this application is used to improve the yield and quality of spring tea and to prevent and / or kill tea pests.

[0030] Preferably, the tea pest is the tea leafroller moth or the tea black tussock moth.

[0031] Compared with the prior art, the beneficial effects of the present invention are:

[0032] The organic fertilizer of this invention uses readily available and abundant raw materials at low cost, making comprehensive use of medicinal plant and microbial resources widely found in nature. Its preparation method is simple, requires no large-scale machinery, and has a promising prospect for widespread application. While improving the yield and quality of spring tea, it also has insecticidal effects and can be used to control pests such as tea leaf rollers and tea black tussock moths. Detailed Implementation

[0033] The technical solution of the present invention will be further described in detail below with reference to specific embodiments.

[0034] Example 1

[0035] An organic fertilizer suitable for increasing tea production and controlling pests comprises the following raw materials in parts by weight: 50 parts straw, 30 parts chicken manure, 15 parts Tibetan incense, 15 parts Clematis armandii, 12 parts Gnaphalium affine, 10 parts Vinegarita, 8 parts ellagic acid-rice protein hydrolysate, 5 parts sodium laurate-modified diatomaceous earth, 5 parts sodium humate, 4 parts urea, 1 part compound microbial bacteria, and 200 parts water;

[0036] The compound microbial strain is composed of Candida utilis and Neurospora intercalans in a mass ratio of 1:2.

[0037] The preparation method of the ellagic acid-rice protein hydrolysate includes the following steps:

[0038] (1) Crush rice, add water and preheat to 40°C, adjust pH to 9, add alkaline protease and hydrolyze for 200 min. After hydrolysis, adjust pH to 8.5, inactivate enzyme, and obtain hydrolysate; the ratio of rice to water is 20 g: 100 mL.

[0039] (2) Dissolve ellagic acid in anhydrous ethanol to obtain ellagic acid solution; wherein the ratio of ellagic acid to anhydrous ethanol is 1g:10mL.

[0040] (3) Mix the enzymatic hydrolysate obtained in step (1) and the ellagic acid solution obtained in step (2), and stir for 60 min to obtain ellagic acid-rice enzymatic hydrolysate.

[0041] The preparation method of the sodium lauryl diatomite modified with sodium lauryl lactone includes the following steps:

[0042] (a) Diatomaceous earth was added to a 10 wt% sodium hydroxide aqueous solution and soaked for 15 min, and then filtered to obtain pretreated diatomaceous earth; wherein the ratio of diatomaceous earth to sodium hydroxide aqueous solution was 1 g: 3 mL.

[0043] (b) Add sodium lauryl nitrate and water to the pretreated diatomite obtained in step (a), stir for 10 min, cool and dry, and then grind to obtain sodium lauryl nitrate modified diatomite; wherein the material-liquid ratio of pretreated diatomite, sodium lauryl nitrate and water is 1 g: 1.5 mL: 20 mL.

[0044] A method for preparing an organic fertilizer suitable for increasing tea yield and controlling pests includes the following steps:

[0045] S1. Weigh the raw materials according to the weight percentages;

[0046] S2. First, crush the Tibetan costus root, Clinacanthus nutans, Gnaphalium affine, and black bone vine into powder, then add sodium humate and water to mix, soak for 100 minutes, then add urea and mix well to obtain the fermentation product.

[0047] S3. Add straw, chicken manure and compound microbial bacteria to the fermentable material obtained in step S2, carry out aerobic fermentation at 25℃ for 10 days, and dry until the moisture content is less than 12wt% to obtain the fermentation product.

[0048] S4. Add ellagic acid-rice protein hydrolysate and sodium lauryl diatomaceous earth modified to the fermentation product obtained in step S3, mix evenly, and granulate to obtain organic fertilizer.

[0049] Example 2

[0050] An organic fertilizer suitable for increasing tea production and controlling pests comprises the following raw materials in parts by weight: 70 parts straw, 40 parts chicken manure, 25 parts Tibetan incense, 20 parts Clematis armandii, 22 parts Gnaphalium affine, 18 parts Vinegarita, 15 parts ellagic acid-rice protein hydrolysate, 10 parts sodium laurate-modified diatomaceous earth, 10 parts sodium humate, 15 parts urea, 6 parts compound microbial bacteria, and 300 parts water;

[0051] The compound microbial strain is composed of Candida utilis and Neurospora intercalans in a mass ratio of 5:7.

[0052] The preparation method of the ellagic acid-rice protein hydrolysate includes the following steps:

[0053] (1) Crush rice, add water and preheat to 50°C, adjust pH to 11, add alkaline protease and hydrolyze for 300 min. After hydrolysis, adjust pH to 9.6 to inactivate the enzyme and obtain hydrolysate. The ratio of rice to water is 30 g: 100 mL.

[0054] (2) Dissolve ellagic acid in anhydrous ethanol to obtain ellagic acid solution; wherein the ratio of ellagic acid to anhydrous ethanol is 1g:20mL.

[0055] (3) Mix the enzymatic hydrolysate obtained in step (1) and the ellagic acid solution obtained in step (2), and stir for 120 min to obtain ellagic acid-rice enzymatic hydrolysate.

[0056] The preparation method of the sodium lauryl diatomite modified with sodium lauryl lactone includes the following steps:

[0057] (a) Diatomaceous earth was soaked in a 15 wt% sodium hydroxide aqueous solution for 40 min and filtered to obtain pretreated diatomaceous earth; wherein the ratio of diatomaceous earth to sodium hydroxide aqueous solution was 1 g: 7 mL.

[0058] (b) Add sodium lauryl nitrate and water to the pretreated diatomite obtained in step (a), stir for 15 minutes, cool and dry, and then grind to obtain sodium lauryl nitrate modified diatomite; wherein the material-liquid ratio of pretreated diatomite, sodium lauryl nitrate and water is 1g:3mL:50mL.

[0059] A method for preparing an organic fertilizer suitable for increasing tea yield and controlling pests includes the following steps:

[0060] S1. Weigh the raw materials according to the weight percentages;

[0061] S2. First, crush the Tibetan costus root, Clinacanthus nutans, Gnaphalium affine, and black bone vine into powder, then add sodium humate and water to mix, soak for 200 minutes, then add urea and mix evenly to obtain the fermentation product.

[0062] S3. Add straw, chicken manure and compound microbial bacteria to the fermentable material obtained in step S2, carry out aerobic fermentation at 28℃ for 20 days, and dry until the moisture content is less than 20wt% to obtain the fermentation product.

[0063] S4. Add ellagic acid-rice protein hydrolysate and sodium lauryl diatomaceous earth modified to the fermentation product obtained in step S3, mix evenly, and granulate to obtain organic fertilizer.

[0064] Example 3

[0065] An organic fertilizer suitable for increasing tea production and controlling pests comprises the following raw materials in parts by weight: 60 parts straw, 35 parts chicken manure, 20 parts Tibetan incense, 18 parts Clematis armandii, 15 parts Gnaphalium affine, 12 parts Vinegarita, 12 parts ellagic acid-rice protein hydrolysate, 8 parts sodium laurate-modified diatomaceous earth, 7 parts sodium humate, 6 parts urea, 5 parts compound microbial bacteria, and 280 parts water;

[0066] The compound microbial strain is composed of Candida utilis and Neurospora intercalans in a mass ratio of 2:5.

[0067] The preparation method of the ellagic acid-rice protein hydrolysate includes the following steps:

[0068] (1) The rice is crushed, water is added and preheated to 45°C, pH is adjusted to 10, alkaline protease is added and hydrolyzed for 250 min. After hydrolysis, pH is adjusted to 9.0 to inactivate the enzyme and obtain hydrolysate. The ratio of rice to water is 27 g: 100 mL.

[0069] (2) Dissolve ellagic acid in anhydrous ethanol to obtain ellagic acid solution; wherein the ratio of ellagic acid to anhydrous ethanol is 1g:15mL.

[0070] (3) Mix the enzymatic hydrolysate obtained in step (1) and the ellagic acid solution obtained in step (2), and stir for 100 min to obtain ellagic acid-rice enzymatic hydrolysate.

[0071] The preparation method of the sodium lauryl diatomite modified with sodium lauryl lactone includes the following steps:

[0072] (a) Diatomaceous earth was added to a 12 wt% sodium hydroxide aqueous solution and soaked for 30 min, and then filtered to obtain pretreated diatomaceous earth; wherein the ratio of diatomaceous earth to sodium hydroxide aqueous solution was 1 g: 5 mL.

[0073] (b) Add sodium lauryl nitrate and water to the pretreated diatomite obtained in step (a), stir for 12 min, cool and dry, and then grind to obtain sodium lauryl nitrate modified diatomite; wherein the material-liquid ratio of pretreated diatomite, sodium lauryl nitrate and water is 1 g: 2 mL: 25 mL.

[0074] A method for preparing an organic fertilizer suitable for increasing tea yield and controlling pests includes the following steps:

[0075] S1. Weigh the raw materials according to the weight percentages;

[0076] S2. First, crush the Tibetan costus root, Clinacanthus nutans, Gnaphalium affine, and black bone vine into powder, then add sodium humate and water to mix, soak for 150 minutes, then add urea and mix well to obtain the fermentation product.

[0077] S3. Add straw, chicken manure and compound microbial bacteria to the fermentation material obtained in step S2, carry out aerobic fermentation at 27°C for 18 days, and dry until the moisture content is less than 18wt% to obtain the fermentation product.

[0078] S4. Add ellagic acid-rice protein hydrolysate and sodium lauryl diatomaceous earth modified to the fermentation product obtained in step S3, mix evenly, and granulate to obtain organic fertilizer.

[0079] Comparative Example 1

[0080] The difference between this comparative example and Example 3 is that the raw material, Tibetan costus root, is replaced with purslane, while the other ingredients and steps remain unchanged.

[0081] An organic fertilizer suitable for increasing tea production and controlling pests comprises the following raw materials in parts by weight: 60 parts straw, 35 parts chicken manure, 20 parts purslane, 18 parts Clematis armandii, 15 parts Gnaphalium affine, 12 parts Gnaphalium affine, 12 parts ellagic acid-rice protein hydrolysate, 8 parts sodium laurate-modified diatomaceous earth, 7 parts sodium humate, 6 parts urea, 5 parts compound microbial bacteria, and 280 parts water;

[0082] The compound microbial strain is composed of Candida utilis and Neurospora intercalans in a mass ratio of 2:5.

[0083] The preparation method of the ellagic acid-rice protein hydrolysate includes the following steps:

[0084] (1) The rice is crushed, water is added and preheated to 45°C, pH is adjusted to 10, alkaline protease is added and hydrolyzed for 250 min. After hydrolysis, pH is adjusted to 9.0 to inactivate the enzyme and obtain hydrolysate. The ratio of rice to water is 27 g: 100 mL.

[0085] (2) Dissolve ellagic acid in anhydrous ethanol to obtain ellagic acid solution; wherein the ratio of ellagic acid to anhydrous ethanol is 1g:15mL.

[0086] (3) Mix the enzymatic hydrolysate obtained in step (1) and the ellagic acid solution obtained in step (2), and stir for 100 min to obtain ellagic acid-rice enzymatic hydrolysate.

[0087] The preparation method of the sodium lauryl diatomite modified with sodium lauryl lactone includes the following steps:

[0088] (a) Diatomaceous earth was added to a 12 wt% sodium hydroxide aqueous solution and soaked for 30 min, and then filtered to obtain pretreated diatomaceous earth; wherein the ratio of diatomaceous earth to sodium hydroxide aqueous solution was 1 g: 5 mL.

[0089] (b) Add sodium lauryl nitrate and water to the pretreated diatomite obtained in step (a), stir for 12 min, cool and dry, and then grind to obtain sodium lauryl nitrate modified diatomite; wherein the material-liquid ratio of pretreated diatomite, sodium lauryl nitrate and water is 1 g: 2 mL: 25 mL.

[0090] A method for preparing an organic fertilizer suitable for increasing tea yield and controlling pests includes the following steps:

[0091] S1. Weigh the raw materials according to the weight percentages;

[0092] S2. First, crush purslane, Clinacanthus nutans, Gnaphalium affine and black bone vine into powder, then add sodium humate and water and mix. After soaking for 150 minutes, add urea and mix well to obtain the fermentation product.

[0093] S3. Add straw, chicken manure and compound microbial bacteria to the fermentation material obtained in step S2, carry out aerobic fermentation at 27°C for 18 days, and dry until the moisture content is less than 18wt% to obtain the fermentation product.

[0094] S4. Add ellagic acid-rice protein hydrolysate and sodium lauryl diatomaceous earth modified to the fermentation product obtained in step S3, mix evenly, and granulate to obtain organic fertilizer.

[0095] Comparative Example 2

[0096] The difference between this comparative example and Example 3 is that the raw material *Clinacanthus nutans* is replaced with *Melia azedarach*, while the other ingredients and steps remain unchanged.

[0097] An organic fertilizer suitable for increasing tea production and controlling pests comprises the following raw materials in parts by weight: 60 parts straw, 35 parts chicken manure, 20 parts Tibetan incense, 18 parts neem, 15 parts cudweed, 12 parts black vine, 12 parts ellagic acid-rice protein hydrolysate, 8 parts sodium laurate modified diatomaceous earth, 7 parts sodium humate, 6 parts urea, 5 parts compound microbial bacteria, and 280 parts water;

[0098] The compound microbial strain is composed of Candida utilis and Neurospora intercalans in a mass ratio of 2:5.

[0099] The preparation method of the ellagic acid-rice protein hydrolysate includes the following steps:

[0100] (1) The rice is crushed, water is added and preheated to 45°C, pH is adjusted to 10, alkaline protease is added and hydrolyzed for 250 min. After hydrolysis, pH is adjusted to 9.0 to inactivate the enzyme and obtain hydrolysate. The ratio of rice to water is 27 g: 100 mL.

[0101] (2) Dissolve ellagic acid in anhydrous ethanol to obtain ellagic acid solution; wherein the ratio of ellagic acid to anhydrous ethanol is 1g:15mL.

[0102] (3) Mix the enzymatic hydrolysate obtained in step (1) and the ellagic acid solution obtained in step (2), and stir for 100 min to obtain ellagic acid-rice enzymatic hydrolysate.

[0103] The preparation method of the sodium lauryl diatomite modified with sodium lauryl lactone includes the following steps:

[0104] (a) Diatomaceous earth was added to a 12 wt% sodium hydroxide aqueous solution and soaked for 30 min, and then filtered to obtain pretreated diatomaceous earth; wherein the ratio of diatomaceous earth to sodium hydroxide aqueous solution was 1 g: 5 mL.

[0105] (b) Add sodium lauryl nitrate and water to the pretreated diatomite obtained in step (a), stir for 12 min, cool and dry, and then grind to obtain sodium lauryl nitrate modified diatomite; wherein the material-liquid ratio of pretreated diatomite, sodium lauryl nitrate and water is 1 g: 2 mL: 25 mL.

[0106] A method for preparing an organic fertilizer suitable for increasing tea yield and controlling pests includes the following steps:

[0107] S1. Weigh the raw materials according to the weight percentages;

[0108] S2. First, crush the Tibetan costus root, bitter melon seed, cudweed and black bone vine into powder, then add sodium humate and water to mix, soak for 150 minutes, then add urea, mix well, and obtain the fermentation product.

[0109] S3. Add straw, chicken manure and compound microbial bacteria to the fermentation material obtained in step S2, carry out aerobic fermentation at 27°C for 18 days, and dry until the moisture content is less than 18wt% to obtain the fermentation product.

[0110] S4. Add ellagic acid-rice protein hydrolysate and sodium lauryl diatomaceous earth modified to the fermentation product obtained in step S3, mix evenly, and granulate to obtain organic fertilizer.

[0111] Comparative Example 3

[0112] The difference between this comparative example and Example 3 is that the raw materials do not contain cudweed, while the other ingredients and steps remain the same.

[0113] An organic fertilizer suitable for increasing tea production and controlling pests comprises the following raw materials in parts by weight: 60 parts straw, 35 parts chicken manure, 20 parts Tibetan incense, 18 parts Clematis armandii, 12 parts Vinegarita 'Black Bone Vine', 12 parts ellagic acid-rice protein hydrolysate, 8 parts sodium laurate modified diatomaceous earth, 7 parts sodium humate, 6 parts urea, 5 parts compound microbial bacteria and 280 parts water;

[0114] The compound microbial strain is composed of Candida utilis and Neurospora intercalans in a mass ratio of 2:5.

[0115] The preparation method of the ellagic acid-rice protein hydrolysate includes the following steps:

[0116] (1) The rice is crushed, water is added and preheated to 45°C, pH is adjusted to 10, alkaline protease is added and hydrolyzed for 250 min. After hydrolysis, pH is adjusted to 9.0 to inactivate the enzyme and obtain hydrolysate. The ratio of rice to water is 27 g: 100 mL.

[0117] (2) Dissolve ellagic acid in anhydrous ethanol to obtain ellagic acid solution; wherein the ratio of ellagic acid to anhydrous ethanol is 1g:15mL.

[0118] (3) Mix the enzymatic hydrolysate obtained in step (1) and the ellagic acid solution obtained in step (2), and stir for 100 min to obtain ellagic acid-rice enzymatic hydrolysate.

[0119] The preparation method of the sodium lauryl diatomite modified with sodium lauryl lactone includes the following steps:

[0120] (a) Diatomaceous earth was added to a 12 wt% sodium hydroxide aqueous solution and soaked for 30 min, and then filtered to obtain pretreated diatomaceous earth; wherein the ratio of diatomaceous earth to sodium hydroxide aqueous solution was 1 g: 5 mL.

[0121] (b) Add sodium lauryl nitrate and water to the pretreated diatomite obtained in step (a), stir for 12 min, cool and dry, and then grind to obtain sodium lauryl nitrate modified diatomite; wherein the material-liquid ratio of pretreated diatomite, sodium lauryl nitrate and water is 1 g: 2 mL: 25 mL.

[0122] A method for preparing an organic fertilizer suitable for increasing tea yield and controlling pests includes the following steps:

[0123] S1. Weigh the raw materials according to the weight percentages;

[0124] S2. First, crush the Tibetan incense, Clinacanthus nutans and black bone vine into powder, then add sodium humate and water to mix, soak for 150 minutes, then add urea and mix evenly to obtain the fermentation product.

[0125] S3. Add straw, chicken manure and compound microbial bacteria to the fermentation material obtained in step S2, carry out aerobic fermentation at 27°C for 18 days, and dry until the moisture content is less than 18wt% to obtain the fermentation product.

[0126] S4. Add ellagic acid-rice protein hydrolysate and sodium lauryl diatomaceous earth modified to the fermentation product obtained in step S3, mix evenly, and granulate to obtain organic fertilizer.

[0127] Comparative Example 4

[0128] The difference between this comparative example and Example 3 is that the raw materials do not contain black bone vine, while the other components and steps remain the same.

[0129] An organic fertilizer suitable for increasing tea production and controlling pests comprises the following raw materials in parts by weight: 60 parts straw, 35 parts chicken manure, 20 parts Tibetan incense, 18 parts Clematis armandii, 15 parts Gnaphalium affine, 12 parts ellagic acid-rice protein hydrolysate, 8 parts sodium laurate-modified diatomaceous earth, 7 parts sodium humate, 6 parts urea, 5 parts compound microbial bacteria, and 280 parts water.

[0130] The compound microbial strain is composed of Candida utilis and Neurospora intercalans in a mass ratio of 2:5.

[0131] The preparation method of the ellagic acid-rice protein hydrolysate includes the following steps:

[0132] (1) The rice is crushed, water is added and preheated to 45°C, pH is adjusted to 10, alkaline protease is added and hydrolyzed for 250 min. After hydrolysis, pH is adjusted to 9.0 to inactivate the enzyme and obtain hydrolysate. The ratio of rice to water is 27 g: 100 mL.

[0133] (2) Dissolve ellagic acid in anhydrous ethanol to obtain ellagic acid solution; wherein the ratio of ellagic acid to anhydrous ethanol is 1g:15mL.

[0134] (3) Mix the enzymatic hydrolysate obtained in step (1) and the ellagic acid solution obtained in step (2), and stir for 100 min to obtain ellagic acid-rice enzymatic hydrolysate.

[0135] The preparation method of the sodium lauryl diatomite modified with sodium lauryl lactone includes the following steps:

[0136] (a) Diatomaceous earth was added to a 12 wt% sodium hydroxide aqueous solution and soaked for 30 min, and then filtered to obtain pretreated diatomaceous earth; wherein the ratio of diatomaceous earth to sodium hydroxide aqueous solution was 1 g: 5 mL.

[0137] (b) Add sodium lauryl nitrate and water to the pretreated diatomite obtained in step (a), stir for 12 min, cool and dry, and then grind to obtain sodium lauryl nitrate modified diatomite; wherein the material-liquid ratio of pretreated diatomite, sodium lauryl nitrate and water is 1 g: 2 mL: 25 mL.

[0138] A method for preparing an organic fertilizer suitable for increasing tea yield and controlling pests includes the following steps:

[0139] S1. Weigh the raw materials according to the weight percentages;

[0140] S2. First, crush the Tibetan costus root, Clinacanthus nutans and Gnaphalium affine, then add sodium humate and water and mix. After soaking for 150 minutes, add urea and mix well to obtain the fermentation product.

[0141] S3. Add straw, chicken manure and compound microbial bacteria to the fermentation material obtained in step S2, carry out aerobic fermentation at 27°C for 18 days, and dry until the moisture content is less than 18wt% to obtain the fermentation product.

[0142] S4. Add ellagic acid-rice protein hydrolysate and sodium lauryl diatomaceous earth modified to the fermentation product obtained in step S3, mix evenly, and granulate to obtain organic fertilizer.

[0143] Comparative Example 5

[0144] The difference between this comparative example and Example 3 is that the raw material ellagic acid-rice protein hydrolysate is replaced with rice protein hydrolysate, while the other components and steps remain unchanged.

[0145] An organic fertilizer suitable for increasing tea production and controlling pests comprises the following raw materials in parts by weight: 60 parts straw, 35 parts chicken manure, 20 parts Tibetan incense, 18 parts Clematis armandii, 15 parts Gnaphalium affine, 12 parts Caulis Amurensis, 12 parts rice protein hydrolysate, 8 parts sodium lauryl latae modified diatomaceous earth, 7 parts sodium humate, 6 parts urea, 5 parts compound microbial bacteria, and 280 parts water;

[0146] The compound microbial strain is composed of Candida utilis and Neurospora intercalans in a mass ratio of 2:5.

[0147] The method for preparing the rice protein hydrolysate includes the following steps: rice is pulverized, water is added and preheated to 45°C, pH is adjusted to 10, alkaline protease is added and hydrolyzed for 250 min, pH is adjusted to 9.0 after hydrolysis, enzyme is inactivated, and rice protein hydrolysate is obtained; wherein the ratio of rice to water is 27 g: 100 mL.

[0148] The preparation method of the sodium lauryl diatomite modified with sodium lauryl lactone includes the following steps:

[0149] (a) Diatomaceous earth was added to a 12 wt% sodium hydroxide aqueous solution and soaked for 30 min, and then filtered to obtain pretreated diatomaceous earth; wherein the ratio of diatomaceous earth to sodium hydroxide aqueous solution was 1 g: 5 mL.

[0150] (b) Add sodium lauryl nitrate and water to the pretreated diatomite obtained in step (a), stir for 12 min, cool and dry, and then grind to obtain sodium lauryl nitrate modified diatomite; wherein the material-liquid ratio of pretreated diatomite, sodium lauryl nitrate and water is 1 g: 2 mL: 25 mL.

[0151] A method for preparing an organic fertilizer suitable for increasing tea yield and controlling pests includes the following steps:

[0152] S1. Weigh the raw materials according to the weight percentages;

[0153] S2. First, crush the Tibetan costus root, Clinacanthus nutans, Gnaphalium affine, and black bone vine into powder, then add sodium humate and water to mix, soak for 150 minutes, then add urea and mix well to obtain the fermentation product.

[0154] S3. Add straw, chicken manure and compound microbial bacteria to the fermentation material obtained in step S2, carry out aerobic fermentation at 27°C for 18 days, and dry until the moisture content is less than 18wt% to obtain the fermentation product.

[0155] S4. Add rice protein hydrolysate and sodium lauryl diatomaceous earth modified with sodium lauryl precipitate to the fermentation product obtained in step S3, mix evenly, and granulate to obtain organic fertilizer.

[0156] Comparative Example 6

[0157] The difference between this comparative example and Example 3 is that the raw materials do not contain ellagic acid-rice protein hydrolysate, while the other components and steps remain unchanged.

[0158] An organic fertilizer suitable for increasing tea production and controlling pests comprises the following raw materials in parts by weight: 60 parts straw, 35 parts chicken manure, 20 parts Tibetan incense, 18 parts Clematis armandii, 15 parts Gnaphalium affine, 12 parts Vinegarita var. rubra, 8 parts sodium lauryl lacquer modified diatomaceous earth, 7 parts sodium humate, 6 parts urea, 5 parts compound microbial bacteria and 280 parts water.

[0159] The compound microbial strain is composed of Candida utilis and Neurospora intercalans in a mass ratio of 2:5.

[0160] The preparation method of the sodium lauryl diatomite modified with sodium lauryl lactone includes the following steps:

[0161] (a) Diatomaceous earth was added to a 12 wt% sodium hydroxide aqueous solution and soaked for 30 min, and then filtered to obtain pretreated diatomaceous earth; wherein the ratio of diatomaceous earth to sodium hydroxide aqueous solution was 1 g: 5 mL.

[0162] (b) Add sodium lauryl nitrate and water to the pretreated diatomite obtained in step (a), stir for 12 min, cool and dry, and then grind to obtain sodium lauryl nitrate modified diatomite; wherein the material-liquid ratio of pretreated diatomite, sodium lauryl nitrate and water is 1 g: 2 mL: 25 mL.

[0163] A method for preparing an organic fertilizer suitable for increasing tea yield and controlling pests includes the following steps:

[0164] S1. Weigh the raw materials according to the weight percentages;

[0165] S2. First, crush the Tibetan costus root, Clinacanthus nutans, Gnaphalium affine, and black bone vine into powder, then add sodium humate and water to mix, soak for 150 minutes, then add urea and mix well to obtain the fermentation product.

[0166] S3. Add straw, chicken manure and compound microbial bacteria to the fermentation material obtained in step S2, carry out aerobic fermentation at 27°C for 18 days, and dry until the moisture content is less than 18wt% to obtain the fermentation product.

[0167] S4. Add sodium lauryl diatomaceous earth modified with sodium lauryl precipitate to the fermentation product obtained in step S3, mix evenly, and granulate to obtain organic fertilizer.

[0168] Comparative Example 7

[0169] The difference between this comparative example and Example 3 is that sodium lauryl diatomaceous earth is replaced with diatomaceous earth, while the other components and steps remain unchanged.

[0170] An organic fertilizer suitable for increasing tea production and controlling pests comprises the following raw materials in parts by weight: 60 parts straw, 35 parts chicken manure, 20 parts Tibetan incense, 18 parts Clematis armandii, 15 parts Gnaphalium affine, 12 parts Caulis Gnaphalium affine, 12 parts Vinegarita 'Black Bone', 12 parts ellagic acid-rice protein hydrolysate, 8 parts diatomaceous earth, 7 parts sodium humate, 6 parts urea, 5 parts compound microbial bacteria, and 280 parts water;

[0171] The compound microbial strain is composed of Candida utilis and Neurospora intercalans in a mass ratio of 2:5.

[0172] The preparation method of the ellagic acid-rice protein hydrolysate includes the following steps:

[0173] (1) The rice is crushed, water is added and preheated to 45°C, pH is adjusted to 10, alkaline protease is added and hydrolyzed for 250 min. After hydrolysis, pH is adjusted to 9.0 to inactivate the enzyme and obtain hydrolysate. The ratio of rice to water is 27 g: 100 mL.

[0174] (2) Dissolve ellagic acid in anhydrous ethanol to obtain ellagic acid solution; wherein the ratio of ellagic acid to anhydrous ethanol is 1g:15mL.

[0175] (3) Mix the enzymatic hydrolysate obtained in step (1) and the ellagic acid solution obtained in step (2), and stir for 100 min to obtain ellagic acid-rice enzymatic hydrolysate.

[0176] A method for preparing an organic fertilizer suitable for increasing tea yield and controlling pests includes the following steps:

[0177] S1. Weigh the raw materials according to the weight percentages;

[0178] S2. First, crush the Tibetan costus root, Clinacanthus nutans, Gnaphalium affine, and black bone vine into powder, then add sodium humate and water to mix, soak for 150 minutes, then add urea and mix well to obtain the fermentation product.

[0179] S3. Add straw, chicken manure and compound microbial bacteria to the fermentation material obtained in step S2, carry out aerobic fermentation at 27°C for 18 days, and dry until the moisture content is less than 18wt% to obtain the fermentation product.

[0180] S4. Add ellagic acid-rice protein hydrolysate and diatomaceous earth to the fermentation product obtained in step S3, mix evenly, and granulate to obtain organic fertilizer.

[0181] Comparative Example 8

[0182] The difference between this comparative example and Example 3 is that sodium lauryl diatomaceous earth is not included in the raw materials, while the other components and steps remain unchanged.

[0183] An organic fertilizer suitable for increasing tea production and controlling pests comprises the following raw materials in parts by weight: 60 parts straw, 35 parts chicken manure, 20 parts Tibetan incense, 18 parts Clematis armandii, 15 parts Gnaphalium affine, 12 parts Vinegarita, 12 parts ellagic acid-rice protein hydrolysate, 7 parts sodium humate, 6 parts urea, 5 parts compound microbial bacteria and 280 parts water;

[0184] The compound microbial strain is composed of Candida utilis and Neurospora intercalans in a mass ratio of 2:5.

[0185] The preparation method of the ellagic acid-rice protein hydrolysate includes the following steps:

[0186] (1) The rice is crushed, water is added and preheated to 45°C, pH is adjusted to 10, alkaline protease is added and hydrolyzed for 250 min. After hydrolysis, pH is adjusted to 9.0 to inactivate the enzyme and obtain hydrolysate. The ratio of rice to water is 27 g: 100 mL.

[0187] (2) Dissolve ellagic acid in anhydrous ethanol to obtain ellagic acid solution; wherein the ratio of ellagic acid to anhydrous ethanol is 1g:15mL.

[0188] (3) Mix the enzymatic hydrolysate obtained in step (1) and the ellagic acid solution obtained in step (2), and stir for 100 min to obtain ellagic acid-rice enzymatic hydrolysate.

[0189] A method for preparing an organic fertilizer suitable for increasing tea yield and controlling pests includes the following steps:

[0190] S1. Weigh the raw materials according to the weight percentages;

[0191] S2. First, crush the Tibetan costus root, Clinacanthus nutans, Gnaphalium affine, and black bone vine into powder, then add sodium humate and water to mix, soak for 150 minutes, then add urea and mix well to obtain the fermentation product.

[0192] S3. Add straw, chicken manure and compound microbial bacteria to the fermentation material obtained in step S2, carry out aerobic fermentation at 27°C for 18 days, and dry until the moisture content is less than 18wt% to obtain the fermentation product.

[0193] S4. Add ellagic acid-rice protein hydrolysate to the fermentation product obtained in step S3, mix well, and granulate to obtain organic fertilizer.

[0194] Comparative Example 9

[0195] The difference between this comparative example and Example 3 is that the Candida utilis in the raw material compound microbial strain is replaced with Bacillus subtilis, while the other components and steps remain unchanged.

[0196] An organic fertilizer suitable for increasing tea production and controlling pests comprises the following raw materials in parts by weight: 60 parts straw, 35 parts chicken manure, 20 parts Tibetan incense, 18 parts Clematis armandii, 15 parts Gnaphalium affine, 12 parts Vinegarita, 12 parts ellagic acid-rice protein hydrolysate, 8 parts sodium laurate-modified diatomaceous earth, 7 parts sodium humate, 6 parts urea, 5 parts compound microbial bacteria, and 280 parts water;

[0197] The composite microbial strain is composed of Bacillus subtilis and Neurospora intercala in a mass ratio of 2:5.

[0198] The preparation method of the ellagic acid-rice protein hydrolysate includes the following steps:

[0199] (1) The rice is crushed, water is added and preheated to 45°C, pH is adjusted to 10, alkaline protease is added and hydrolyzed for 250 min. After hydrolysis, pH is adjusted to 9.0 to inactivate the enzyme and obtain hydrolysate. The ratio of rice to water is 27 g: 100 mL.

[0200] (2) Dissolve ellagic acid in anhydrous ethanol to obtain ellagic acid solution; wherein the ratio of ellagic acid to anhydrous ethanol is 1g:15mL.

[0201] (3) Mix the enzymatic hydrolysate obtained in step (1) and the ellagic acid solution obtained in step (2), and stir for 100 min to obtain ellagic acid-rice enzymatic hydrolysate.

[0202] The preparation method of the sodium lauryl diatomite modified with sodium lauryl lactone includes the following steps:

[0203] (a) Diatomaceous earth was added to a 12 wt% sodium hydroxide aqueous solution and soaked for 30 min, and then filtered to obtain pretreated diatomaceous earth; wherein the ratio of diatomaceous earth to sodium hydroxide aqueous solution was 1 g: 5 mL.

[0204] (b) Add sodium lauryl nitrate and water to the pretreated diatomite obtained in step (a), stir for 12 min, cool and dry, and then grind to obtain sodium lauryl nitrate modified diatomite; wherein the material-liquid ratio of pretreated diatomite, sodium lauryl nitrate and water is 1 g: 2 mL: 25 mL.

[0205] A method for preparing an organic fertilizer suitable for increasing tea yield and controlling pests includes the following steps:

[0206] S1. Weigh the raw materials according to the weight percentages;

[0207] S2. First, crush the Tibetan costus root, Clinacanthus nutans, Gnaphalium affine, and black bone vine into powder, then add sodium humate and water to mix, soak for 150 minutes, then add urea and mix well to obtain the fermentation product.

[0208] S3. Add straw, chicken manure and compound microbial bacteria to the fermentable material obtained in step S2, carry out aerobic fermentation at 27°C for 18 days, and dry until the moisture content is less than 18wt% to obtain the fermentation product.

[0209] S4. Add ellagic acid-rice protein hydrolysate and sodium lauryl diatomaceous earth modified to the fermentation product obtained in step S3, mix evenly, and granulate to obtain organic fertilizer.

[0210] Comparative Example 10

[0211] The difference between this comparative example and Example 3 is that the *Neurospora intercala* in the raw material compound microbial strain is replaced with *Trichoderma hygroscopica*, while the other components and steps remain unchanged.

[0212] An organic fertilizer suitable for increasing tea production and controlling pests comprises the following raw materials in parts by weight: 60 parts straw, 35 parts chicken manure, 20 parts Tibetan incense, 18 parts Clematis armandii, 15 parts Gnaphalium affine, 12 parts Vinegarita, 12 parts ellagic acid-rice protein hydrolysate, 8 parts sodium laurate-modified diatomaceous earth, 7 parts sodium humate, 6 parts urea, 5 parts compound microbial bacteria, and 280 parts water;

[0213] The compound microbial strain is composed of Candida utilis and Trichoderma echinosporum in a mass ratio of 2:5.

[0214] The preparation method of the ellagic acid-rice protein hydrolysate includes the following steps:

[0215] (1) The rice is crushed, water is added and preheated to 45°C, pH is adjusted to 10, alkaline protease is added and hydrolyzed for 250 min. After hydrolysis, pH is adjusted to 9.0 to inactivate the enzyme and obtain hydrolysate. The ratio of rice to water is 27 g: 100 mL.

[0216] (2) Dissolve ellagic acid in anhydrous ethanol to obtain ellagic acid solution; wherein the ratio of ellagic acid to anhydrous ethanol is 1g:15mL.

[0217] (3) Mix the enzymatic hydrolysate obtained in step (1) and the ellagic acid solution obtained in step (2), and stir for 100 min to obtain ellagic acid-rice enzymatic hydrolysate.

[0218] The preparation method of the sodium lauryl diatomite includes the following steps:

[0219] (a) Diatomaceous earth was added to a 12 wt% sodium hydroxide aqueous solution and soaked for 30 min, and then filtered to obtain pretreated diatomaceous earth; wherein the ratio of diatomaceous earth to sodium hydroxide aqueous solution was 1 g: 5 mL.

[0220] (b) Add sodium lauryl nitrate and water to the pretreated diatomite obtained in step (a), stir for 12 min, cool and dry, and then grind to obtain sodium lauryl nitrate modified diatomite; wherein the material-liquid ratio of pretreated diatomite, sodium lauryl nitrate and water is 1 g: 2 mL: 25 mL.

[0221] A method for preparing an organic fertilizer suitable for increasing tea yield and controlling pests includes the following steps:

[0222] S1. Weigh the raw materials according to the weight percentages;

[0223] S2. First, crush the Tibetan costus root, Clinacanthus nutans, Gnaphalium affine, and black bone vine into powder, then add sodium humate and water to mix, soak for 150 minutes, then add urea and mix well to obtain the fermentation product.

[0224] S3. Add straw, chicken manure and compound microbial bacteria to the fermentable material obtained in step S2, carry out aerobic fermentation at 27°C for 18 days, and dry until the moisture content is less than 18wt% to obtain the fermentation product.

[0225] S4. Add ellagic acid-rice protein hydrolysate and sodium lauryl diatomaceous earth modified to the fermentation product obtained in step S3, mix evenly, and granulate to obtain organic fertilizer.

[0226] Experiment 1: Increasing Tea Yield

[0227] 1.1 Test Methods

[0228] Tea gardens harvested only from Huangshan Maofeng tea in spring were selected and divided into 42 equal-sized plots, each 4 mu (approximately 0.67 hectares). These plots were further divided into experimental groups (Examples 1-3), comparative groups (Comparative Examples 1-10), and control groups (conventional fertilizer: N:P2O5:K2O:MgO = 22:8:10:2). Each group underwent three parallel trials. The yield of tea leaves was recorded after each harvest. Simultaneously, the contents of free amino acids (determined using the ninhydrin colorimetric method (GB / T8314-2002), tea polyphenols (determined using the ferric tartrate colorimetric method (GB / T8313-2002),) and caffeine (determined using ultraviolet spectrophotometry (GB / T8312-2002)) were measured. The results are shown in Table 1.

[0229] 1.2 Tea yield and quality

[0230] Table 1 Average tea yield and tea quality

[0231] Average yield of fresh tea leaves per mu (unit of land area) / kg Free amino acids / % Tea polyphenols % caffeine % control group 41.3 2.1 21.2 2.0 Example 1 64.5 4.4 25.0 4.1 Example 2 65.0 4.5 25.1 4.2 Example 3 65.2 4.5 25.3 4.5 Comparative Example 1 58.6 4.3 24.8 3.7 Comparative Example 2 60.1 4.3 25.0 3.9 Comparative Example 3 44.2 3.2 22.6 2.6 Comparative Example 4 43.8 2.9 22.0 2.6 Comparative Example 5 50.1 3.7 23.5 2.8 Comparative Example 6 47.0 3.4 22.8 2.7 Comparative Example 7 51.7 3.8 24.0 3.1 Comparative Example 8 47.9 3.5 23.0 2.7 Comparative Example 9 55.1 4.1 24.6 3.6 Comparative Example 10 54.4 3.9 24.3 3.4

[0232] Table 1 shows that the organic fertilizers of Examples 1-3 of this invention resulted in optimal average fresh tea yield, free amino acid content, tea polyphenol content, and caffeine content per acre. Comparison of Example 3 with Comparative Examples 1-4 shows that the synergistic effect of *Saussurea costus*, *Clematis chinensis*, *Gnaphalium affine*, and *Gnaphalium affine* in improving tea yield and quality is crucial; the absence or substitution of any single ingredient leads to a decrease in effectiveness. Comparison of Example 3 with Comparative Examples 5-8 shows that replacing ellagic acid-rice protein hydrolysate with rice protein hydrolysate and sodium laurate-modified diatomaceous earth with diatomaceous earth results in a decrease in tea yield. The combination of ellagic acid and rice protein hydrolysate synergistically enhances the stability of the organic fertilizer and maintains the biological activity of ellagic acid. The sodium laurate-modified diatomaceous earth... Diatomaceous earth, through surface modification, further enhances the adsorption capacity of diatomaceous earth, optimizes the release of nutrients in the soil, maintains the dynamic balance of soil fertility, and thus improves the absorption efficiency and growth rate of tea roots. As can be seen from the comparison between Example 3 and Comparative Examples 9 and 10, the selection of suitable aerobic bacteria is particularly important. Candida utilis can decompose macromolecular compounds such as proteins and cellulose, converting them into small molecules such as amino acids and monosaccharides. Neurospora intercalans accelerates the degradation of raw materials in organic fertilizers by secreting extracellular enzymes, releasing active ingredients, thereby improving the soil fertility environment and the tea growth environment.

[0233] Insecticide effect in Experiment Example 2

[0234] 2.1 Experimental materials: The organic fertilizers prepared in Examples 1 to 3 were mixed with water at a ratio of 1g:3mL. After stirring for 20 minutes, the mixture was filtered to obtain an organic fertilizer aqueous solution, which was used to test the insecticidal effect; 2.5% deltamethrin.

[0235] 2.2 Test Methods: Determination of contact toxicity against tea leafroller moth and tea black tussock moth

[0236] Methods: The leaf-disc method was used for determination. Fresh, pesticide-free Huangshan Maofeng tea leaves were taken, and holes were punched in the leaves to make circular leaf discs with a diameter of 10 cm. The leaves were immersed in the test material for 5 minutes and then dried. Thirty tea leafroller moths and thirty tea black tussock moths were inoculated into the leaves respectively. The discs were placed in an incubator with a light / dark cycle of 15 h / 8 h, a temperature of 25 °C, and a humidity of 60-70%. The experimental group consisted of leaves immersed in the organic fertilizer aqueous solution prepared in this application, the blank control group consisted of leaves immersed in clean water, and the positive control group consisted of leaves immersed in 2.5% deltamethrin. Each treatment was repeated three times. After 24 h of treatment, the mortality and survival of tea leafroller moths and tea black tussock moths in each treatment were checked, and the mortality rate was calculated.

[0237] (3) Test Results

[0238] As shown in Tables 2 and 3:

[0239] Table 2 Results of contact toxicity assay against tea leafroller moth

[0240] Serial Number Number of survivors Mortality rate Example 1 3 90.00％ Example 2 3 90.00％ Example 3 2 93.33％ Positive control group 5 83.33％ Blank control group 30 0.00％

[0241] Table 3 Results of contact toxicity assay against the tea black tussock moth.

[0242] Serial Number Number of survivors Mortality rate Example 1 6 80.00％ Example 2 6 80.00％ Example 3 4 86.67％ Positive control group 8 73.33％ Blank control group 30 0.00％

[0243] As shown in the table above, the organic fertilizer aqueous solutions prepared in Examples 1-3 all had a mortality rate of 80% or higher against the tea leafroller moth and the tea black tussock moth, with Example 3 showing the best insecticidal effect. Compared with the positive control group, Example 3 showed that the insecticide of the present invention was more effective than deltamethrin, a commonly recognized insecticide in the art.

[0244] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present invention should be included within the protection scope of the present invention.

Claims

1. An organic fertilizer suitable for increasing tea yield and controlling pests, characterized in that it improves the yield and quality of spring tea and prevents and / or kills tea pests. The organic fertilizer comprises the following raw materials in parts by weight: 50-70 parts straw, 30-40 parts chicken manure, 15-25 parts Tibetan incense, 15-20 parts Clinacanthus nutans, 12-22 parts Gnaphalium affine, 10-18 parts Black vine, 8-15 parts ellagic acid-rice protein hydrolysate, 5-10 parts sodium laurate-modified diatomaceous earth, 5-10 parts sodium humate, 4-15 parts urea, 1-6 parts compound microbial inoculum, and 200-300 parts water; The compound microbial strain is composed of Candida utilis and Neurospora intercala in a mass ratio of (1~5):(2~7); The preparation method of the ellagic acid-rice protein hydrolysate includes the following steps: (1) Crush rice, add water and preheat to 40~50℃, adjust pH to 9~11, add alkaline protease and hydrolyze for 200~300min, after hydrolysis, adjust pH to 8.5~9.6, inactivate enzyme, and obtain hydrolysate; (2) Dissolve ellagic acid in anhydrous ethanol to obtain an ellagic acid solution; (3) Mix the enzymatic hydrolysate obtained in step (1) and the ellagic acid solution obtained in step (2), and stir for 60~120 min to obtain ellagic acid-rice enzymatic hydrolysate; The preparation method of the sodium lauryl diatomite modified with sodium lauryl lactone includes the following steps: (a) Add diatomaceous earth to a 10-15 wt% sodium hydroxide aqueous solution and soak for 15-40 min. The material-to-liquid ratio is 1 g: (3-7) mL. Filter to obtain pretreated diatomaceous earth. (b) Add sodium lauryl nitrate and water to the pretreated diatomite obtained in step (a), stir for 10-15 minutes, cool and dry, and then grind to obtain sodium lauryl nitrate modified diatomite. The tea pests mentioned are the tea leafroller moth or the tea black tussock moth.

2. The application according to claim 1, characterized in that, The organic fertilizer comprises the following raw materials in parts by weight: 60 parts straw, 35 parts chicken manure, 20 parts Tibetan incense, 18 parts Clematis armandii, 15 parts Gnaphalium affine, 12 parts Caulis Gnaphalium affine, 12 parts Vitis thunbergii, 12 parts ellagic acid-rice protein hydrolysate, 8 parts sodium laurate-modified diatomaceous earth, 7 parts sodium humate, 6 parts urea, 5 parts compound microbial inoculants, and 280 parts water; The compound microbial strain is composed of Candida utilis and Neurospora intercalans in a mass ratio of 2:

5.

3. The application according to claim 1, characterized in that, In step (1), the ratio of rice to water is (20~30) g: 100 mL.

4. The application according to claim 1, characterized in that, In step (2), the ratio of ellagic acid to anhydrous ethanol is 1g:(10~20)mL.

5. The application according to claim 1, characterized in that, In step (b), the ratio of pretreated diatomaceous earth, sodium lauryl laurate and water is 1g:(1.5~3)mL:(20~50)mL.

6. The application according to claim 1, characterized in that, The method for preparing the organic fertilizer includes the following steps: S1. Weigh the raw materials according to the weight proportions specified in claim 1; S2. First, crush the Tibetan costus root, Clinacanthus nutans, Gnaphalium affine, and black bone vine into powder, then add sodium humate and water to mix, soak for 100-200 minutes, then add urea, mix well, and obtain the fermentation product. S3. Add straw, chicken manure and compound microbial bacteria to the fermentable material obtained in step S2, carry out aerobic fermentation at a temperature of 25~28℃ for 10~20 days, and dry until the moisture content is less than 12~20wt% to obtain the fermentation product. S4. Add ellagic acid-rice protein hydrolysate and sodium lauryl diatomaceous earth modified to the fermentation product obtained in step S3, mix evenly, and granulate to obtain organic fertilizer.