Full fermentation period starch hydrolysis ultrafast reaction kinetic measurement device and measurement method thereof

Abstract

The invention belongs to the field of biochemical reaction kinetics and chemical measurement, and discloses a full-fermentation-period starch hydrolysis ultrafast reaction kinetics measuring device and a measuring method thereof. The high-repetition-frequency ultrafast laser is adopted as a detection light source, femtosecond-order ultrafast biochemical reaction dynamic behaviors in a reaction system can be continuously captured, jitter suppression is carried out on the detection light source through the waveform shaper, so that detection signals of the biochemical reaction system can be continuously kept stable in a full fermentation period as long as several months, and the detection accuracy is improved. All transverse mode phases in the pulse can be completely the same by eliminating chirp, distortion of phase distribution in the pulse completely comes from modulation of a biochemical reaction system, time domain stretching and spectral domain stretching are carried out on an ultrafast signal, signal measurement difficulty and cost can be reduced, reconstruction of an ultrafast pulse field eigenstate is more accurate, and the method is suitable for large-scale popularization and application. Therefore, the measurement and characterization precision of the system on ultrafast reaction kinetics is improved.

Description

Technical Field

[0001] The invention belongs to the field of biochemical reaction kinetics and chemical measurement, and particularly relates to a device for measuring ultrafast reaction kinetics of starch hydrolysis during a full fermentation cycle and a measuring method thereof. Background Art

[0002] Starch hydrolysis is a fundamental biochemical reaction in food fermentation, and real-time monitoring of its hydrolysis progress is crucial for regulating the quality of fermentation products. Currently, monitoring the microscopic molecular structure of starch in fermentation systems relies primarily on periodic sampling. This method requires offline testing and can only indirectly infer the hydrolysis process from the physicochemical parameters of reactants and products, preventing in situ real-time monitoring. The starch hydrolysis reaction exhibits multi-stage enzymatic catalysis, with ultrafast reaction kinetics encompassing sub-nanosecond conformational changes in the enzyme-substrate complex, femtosecond transition state kinetics of glycosidic bond cleavage, and millisecond product release and diffusion. Among existing detection technologies, stopped-flow spectroscopy triggers the reaction through mechanical mixing and measures changes in the transmission spectrum to obtain reaction kinetic data. Ultrafast pump-probe techniques utilize femtosecond laser pulses to probe the relaxation of molecular excited states to reveal the hydrolysis reaction dynamics. However, stopped-flow spectroscopy is limited by its mechanical triggering mechanism and cannot perform real-time measurements of initiated continuous reactions. Ultrafast pump-probe techniques, based on coherent detection, struggle to resolve non-periodic reaction characteristics. From the perspective of measurement system performance, existing photoelectric detection equipment operates at gigahertz sampling frequencies, while the characteristic events of ultrafast biochemical reactions occur at terahertz frequencies, resulting in a temporal resolution gap of approximately three orders of magnitude. Furthermore, existing ultrafast detection devices are limited by laser stability and optical system drift, making it impossible to maintain signal stability over the multi-month fermentation cycle. This makes long-term, continuous monitoring of ultrafast biochemical reaction kinetics impossible. Summary of the Invention

[0003] In order to overcome the above-mentioned shortcomings and deficiencies of the prior art, the primary purpose of the present invention is to provide a device for measuring ultrafast reaction kinetics of starch hydrolysis during the entire fermentation cycle.

[0004] Another object of the present invention is to provide a method for measuring the ultrafast reaction kinetics of starch hydrolysis throughout the fermentation cycle. This method uses ultrafast ground-state soliton pulses generated by a high-repetition-rate ultrafast laser as an optical probe. This method can capture in real time the modulation of the pulse's time-frequency characteristics by changes in the microscopic molecular structure caused by chemical changes during starch hydrolysis, thereby enabling precise monitoring and quantitative characterization of ultrafast biochemical reaction kinetics.

[0005] The purpose of the present invention is achieved through the following solutions:

[0006] A device for measuring the ultrafast reaction kinetics of starch hydrolysis during the entire fermentation cycle includes a high-repetition-rate ultrafast laser module, a biochemical reactor module, an ultrafast biochemical reaction kinetics measurement module, and a data processing module. The output of the high-repetition-rate ultrafast laser module is connected to the input of the biochemical reactor module, which in turn is connected to the input of the ultrafast biochemical reaction kinetics measurement module. The output of the ultrafast biochemical reaction kinetics measurement module is connected to the input of the data processing module. Specifically:

[0007] The high repetition rate ultrafast laser module is used to generate high repetition rate ultrafast ground state soliton pulses;

[0008] The biochemical reactor module is used for fermentation reaction and is provided with a sampling window to realize real-time monitoring of the reaction process;

[0009] The ultrafast biochemical reaction kinetics measurement module is used to perform time domain stretching and spectral domain stretching on ultrafast pulses modulated by the biochemical reaction system, and to collect pulse transient evolution data sets in real time;

[0010] The data processing module is used to execute an ultrafast pulse field inversion algorithm, reconstruct ultrafast pulse field eigenstate information based on a pulse transient evolution data set, and reconstruct the ultrafast reaction kinetics of starch hydrolysis from this information using an artificial intelligence algorithm.

[0011] Furthermore, the high repetition rate ultrafast laser module includes a pumping light source, a wavelength division multiplexer, a gain fiber, a first fiber coupler, a waveform shaper, a fiber isolator, and a polarization controller. The output end of the pumping light source is connected to the shortwave input end of the wavelength division multiplexer, the output end of the wavelength division multiplexer is connected to the input end of the gain fiber, the output end of the gain fiber is connected to the input end of the first fiber coupler, the large-scale output end of the first fiber coupler is connected to the input end of the waveform shaper, the output end of the waveform shaper is connected to the input end of the fiber isolator, the output end of the fiber isolator is connected to the input end of the polarization controller, the output end of the polarization controller is connected to the longwave input end of the wavelength division multiplexer, and the small-scale output end of the first fiber coupler is connected to the input end of the biochemical reactor module, wherein:

[0012] The gain optical fiber is used to generate and amplify laser signals in a specific working band;

[0013] The pumping light source is used to generate a pumping laser signal, provide energy to the working material in the gain fiber, stimulate its energy level transition, and thus generate a laser signal in the working band;

[0014] The polarization controller is used to adjust the polarization state of the high repetition rate ultrafast laser module;

[0015] The wavelength division multiplexer combines the pumping laser signal with the working wavelength band laser signal output by the polarization controller, and injects the combined laser into the gain optical fiber;

[0016] The first fiber coupler is used to split the laser signal into two paths with a splitting ratio ranging from 1:99 to 45:55. The path with a smaller ratio is transmitted as the output laser signal to the biochemical reactor module, and the path with a larger ratio is returned as the echo signal to the waveform shaper of the high repetition rate ultrafast laser module.

[0017] The waveform shaper is used to perform chirp elimination and jitter suppression on the laser signal running in the high repetition rate ultrafast laser module, so that the output laser signal is stabilized in the ultrafast ground state soliton pulse state;

[0018] The optical fiber isolator is used to guide the running direction of the laser signal in the high repetition rate ultrafast laser module.

[0019] Furthermore, the biochemical reactor module includes a fermentation tank and a sampling window, the output end of the first fiber coupler in the high repetition rate ultrafast laser module is connected to the input end of the sampling window, the sampling window is in communication with the interior of the fermentation tank, and the output end of the sampling window is connected to the input end of the ultrafast biochemical reaction kinetics measurement module, wherein:

[0020] The fermentation tank is used to provide a container for the reaction system to carry out biochemical reactions;

[0021] The sampling window is used to provide a monitoring window for the reaction system, allowing the laser signal generated by the high repetition rate ultrafast laser module to penetrate the reaction system and couple the transmitted light to the optical fiber end face at the output end on the other side of the sampling window, so that the signal can be transmitted to the ultrafast biochemical reaction kinetics measurement module.

[0022] Furthermore, the ultrafast biochemical reaction kinetics measurement module includes a second optical fiber coupler, a time domain stretching device, a spectral domain stretching device, a first photodetector, a second photodetector, and an analog-to-digital converter. The output end of the biochemical reactor module is connected to the input end of the second optical fiber coupler, the first output end of the second optical fiber coupler is connected to the input end of the time domain stretching device, the output end of the time domain stretching device is connected to the input end of the first photodetector, the output end of the first photodetector is connected to the first input end of the analog-to-digital converter, the second output end of the second optical fiber coupler is connected to the input end of the spectral domain stretching device, the output end of the spectral domain stretching device is connected to the input end of the second photodetector, the output end of the second photodetector is connected to the second input end of the analog-to-digital converter, and the output end of the analog-to-digital converter is connected to the input end of the data processing module.

[0023] The second optical fiber coupler is used to perform optical splitting processing on the laser signal modulated by the reaction system to generate a signal to be stretched in the time domain and a signal to be stretched in the spectral domain;

[0024] The time domain stretching device is used to stretch the time scale of the laser signal in the time domain to generate a pulsed time domain stretched laser signal;

[0025] The spectral domain stretching device is used to map the spectral domain information of the pulse to the time domain, and improve the spectral domain measurement resolution by stretching the time scale of the signal, thereby generating a pulsed spectral domain stretched laser signal;

[0026] The first photodetector is used to perform photoelectric conversion on the pulse time-domain stretching laser signal to generate a pulse time-domain stretching analog electronic response signal;

[0027] The second photodetector is used to perform photoelectric conversion on the pulsed spectrum domain stretching laser signal to generate a pulsed spectrum domain stretching analog electronic response signal;

[0028] The analog-to-digital converter is used to perform analog-to-digital conversion on the pulse time domain stretching analog electronic response signal and the pulse spectrum domain stretching analog electronic response signal, respectively, to generate a pulse time domain stretching electronic response digital signal and a pulse spectrum domain stretching electronic response digital signal, respectively.

[0029] Furthermore, the data processing module includes a server, the input end of the server is connected to the output end of the ultrafast biochemical reaction kinetics measurement module, wherein:

[0030] The server is used to reconstruct ultrafast pulse field eigenstate information based on the pulse time domain stretched electron response digital signal and the pulse spectrum domain stretched electron response digital signal, and reconstruct the ultrafast biochemical reaction kinetics of starch hydrolysis.

[0031] A method for measuring ultrafast reaction kinetics of starch hydrolysis during a full fermentation cycle comprises the following steps:

[0032] Obtain high repetition rate ultrafast ground state soliton pulse signals;

[0033] The ultrafast ground state soliton pulse signal is modulated by the reaction system to obtain a modulated signal;

[0034] By performing time domain stretching processing and spectral domain stretching processing on the modulated signal and performing data acquisition, a pulse transient evolution data set is obtained, wherein the pulse transient evolution data set includes a pulse time domain dynamics data set and a pulse spectral domain dynamics data set;

[0035] Inverse the pulse transient evolution data set to obtain the pulse field eigenstate dynamics data set;

[0036] The pulsed field eigenstate kinetics dataset was analyzed to obtain an ultrafast reaction kinetics dataset of starch hydrolysis.

[0037] Furthermore, the step of obtaining a high repetition rate ultrafast ground state soliton pulse signal specifically includes:

[0038] By adjusting the polarization controller and pump light source settings, a conventional high repetition rate ultrafast pulse signal is obtained;

[0039] A waveform shaper is used to eliminate pulse chirp and jitter to obtain a high repetition rate ultrafast ground-state soliton pulse signal.

[0040] Furthermore, the step of modulating the ultrafast ground state soliton pulse signal through the fermentation and hydrolysis reaction system to obtain a modulated signal specifically includes:

[0041] The ultrafast ground-state soliton pulse signal is emitted into the fermentation and hydrolysis reaction system through a fully transparent sample window, so that the microscopic molecular changes in the fermentation and hydrolysis system can modulate the ultrafast ground-state soliton pulse signal;

[0042] The transmitted modulated signal is coupled to the optical fiber end face on the other side of the sample window, so that the modulated signal is transmitted to the ultrafast biochemical reaction kinetics measurement module.

[0043] Furthermore, the step of performing time domain stretching processing and spectral domain stretching processing on the modulated signal and performing data acquisition to obtain a pulse transient evolution data set specifically includes:

[0044] The modulated signal is subjected to distortion-free time domain stretching and distortion-free spectral domain stretching respectively using time lens technology and dispersive Fourier transform technology, until the generated pulse time domain stretching signal and pulse spectral domain stretching signal can be accurately measured by conventional photodetectors;

[0045] A photodetector is used to convert the pulse time domain stretching signal and the pulse spectrum domain stretching signal into a pulse time domain stretching simulated electronic response signal and a pulse spectrum domain stretching simulated electronic response signal respectively;

[0046] An analog-to-digital converter is used to convert the pulse time-domain stretching analog electronic response signal and the pulse spectrum domain stretching analog electronic response signal into a pulse time-domain stretching digital electronic response signal and a pulse spectrum domain stretching digital electronic response signal respectively;

[0047] The server is used to collect the pulse time domain stretched digital electronic response signal and the pulse spectral domain stretched digital electronic response signal to obtain the pulse time domain dynamics dataset and the pulse spectral domain dynamics dataset, respectively, which constitute the pulse transient evolution dataset.

[0048] Furthermore, the step of performing inversion processing on the pulse transient evolution data set to obtain the pulse field eigenstate dynamics data set specifically includes:

[0049] The phase inversion algorithm is used to invert the pulse spectral domain phase dynamics dataset based on the pulse transient evolution dataset, and the pulse spectral domain dynamics dataset is further combined with the pulse spectral domain phase dynamics dataset to reconstruct the pulse field eigenstate dynamics dataset.

[0050] Furthermore, the step of analyzing the pulsed field eigenstate kinetics dataset to obtain the starch hydrolysis ultrafast reaction kinetics dataset specifically includes:

[0051] Using artificial intelligence algorithms, the ultrafast reaction kinetics dataset of starch hydrolysis was obtained by inputting the ultrafast ground state soliton pulse field eigenstate dynamics dataset before and after modulation of the reaction system into the neural network.

[0052] Compared with the prior art, the present invention has the following advantages and beneficial effects:

[0053] The present invention adopts a high-repetition-rate ultrafast laser as the detection light source, which can continuously capture the ultrafast biochemical reaction dynamics behavior of the femtosecond level in the reaction system. The waveform shaper suppresses the jitter of the detection light source, so that the detection signal of the biochemical reaction system can remain stable during the full fermentation cycle of several months. By eliminating chirp, the phases of all transverse modes within the pulse can be made exactly the same, and thus the distortion of the phase distribution within the pulse is completely derived from the modulation of the biochemical reaction system. Time domain stretching and spectral domain stretching of the ultrafast signal are beneficial to reducing the difficulty and cost of signal measurement, making the reconstruction of the eigenstate of the ultrafast pulse field more accurate, and thus improving the system's measurement and characterization accuracy of ultrafast reaction dynamics.

[0054] Specifically, the present invention achieves more accurate reconstruction of pulse field eigenstates by incorporating multi-physics information domain data for constraints. Conventional detection methods typically only detect in the spectral domain, making it impossible to uniquely determine the pulse phase, further resulting in an inability to accurately detect ultrafast biochemical reactions. Assuming that the ultrafast pulse field contains a single-frequency component with an angular frequency of ω1, the angular frequency can be calculated using the following formula:

[0055]

[0056] where φ1 and Represent the total phase and initial phase respectively. Solving the above equation we can get:

[0057] φ1=∫ω1dt=ω1t+C

[0058] Where C is an arbitrary constant. However, the original initial phase It is not an arbitrary constant, which means that even if the spectra of two pulses are exactly the same, their phases are not always the same. According to the expression of the ultrafast pulse field:

[0059]

[0060] Among them, A n 、ω n and Represent the amplitude, angular frequency and initial phase of the nth frequency component respectively, and Ψ(t) represents the complex amplitude of the pulse field. Through time domain detection, Ψ(t) is a known quantity; through spectral domain detection, A n and ω n are also known quantities, then the formula only has The phase of the ultrafast pulse field is unknown. Therefore, by combining the time-domain and spectral-domain measurements, we can solve the equation to uniquely determine the phase of the ultrafast pulse field. Substituting the results into the equation, we can reconstruct an analytical expression for the ultrafast pulse field. BRIEF DESCRIPTION OF THE DRAWINGS

[0061] Figure 1 This is a schematic structural diagram of a device for measuring ultrafast reaction kinetics of starch hydrolysis during the entire fermentation cycle of the present invention;

[0062] Figure 2 This is a schematic flow chart of the steps of a measurement method of a device for measuring ultrafast reaction kinetics of starch hydrolysis during a full fermentation cycle of the present invention;

[0063] Figure 3 This is a schematic diagram of measurement results of a device for measuring ultrafast reaction kinetics of starch hydrolysis during a full fermentation cycle according to the present invention.

[0064] Explanation of the accompanying symbols: A. high repetition rate ultrafast laser module; B. biochemical reactor module; C. ultrafast biochemical reaction kinetics measurement module; D. data processing module; 1. pumping light source; 2. wavelength division multiplexer; 3. gain fiber; 4a. first fiber coupler; 4b. second fiber coupler; 5. waveform shaper; 6. fiber isolator; 7. polarization controller; 8. fermenter; 9. sampling window; 10. time domain stretching device; 11. spectral domain stretching device; 12a. first photodetector; 12b. second photodetector; 13. analog-to-digital converter; 14. server. DETAILED DESCRIPTION

[0065] The present invention will be described in further detail below with reference to the Examples and accompanying drawings, but the embodiments of the present invention are not limited thereto. Where specific conditions are not specified in the Examples, conventional conditions or conditions recommended by the manufacturer were followed. Reagents or instruments used, where the manufacturer is not specified, are commercially available conventional products.

[0066] Example 1

[0067] The mechanism by which the embodiments of the present invention achieve ultrafast reaction kinetics measurement of starch hydrolysis throughout the entire fermentation cycle is as follows: using high-repetition-rate ultrafast ground-state soliton pulses as detection signals, modulating the detection signals using the dynamic electromagnetic response brought about by changes in the microstructure of the material in the reaction system, and accurately measuring the modulated signals using time-domain stretching and spectral-domain stretching techniques to obtain a pulse time-domain kinetics dataset and a pulse spectral-domain kinetics dataset. The pulse field eigenstate kinetics dataset is then inverted based on the pulse transient kinetics dataset to determine the modulation effect of the reaction system on the pulse field eigenstate. Furthermore, an artificial intelligence algorithm is used to parse the ultrafast biochemical reaction kinetics occurring in the starch hydrolysis reaction system from the pulse field eigenstate kinetics dataset, and to plot in real time the evolutionary kinetics of multi-scale features such as starch chain length distribution, weight-average molecular weight, and molecular weight distribution during the fermentation cycle.

[0068] Reference Figure 1 The present invention provides a device for measuring ultrafast reaction kinetics of starch hydrolysis during a full fermentation cycle, comprising a high-repetition-rate ultrafast laser module A, a biochemical reactor module B, an ultrafast biochemical reaction kinetics measurement module C, and a data processing module D. The output end of the high-repetition-rate ultrafast laser module A is connected to the input end of the biochemical reactor module B, the output end of the biochemical reactor module B is connected to the input end of the ultrafast biochemical reaction kinetics measurement module C, and the output end of the ultrafast biochemical reaction kinetics measurement module C is connected to the input end of the data processing module D.

[0069] High repetition rate ultrafast laser module A is used to generate high repetition rate ultrafast ground state soliton pulses;

[0070] Specifically, the high repetition rate ultrafast laser module A includes a pumping light source 1, a wavelength division multiplexer 2, a gain fiber 3, a first fiber coupler 4a, a waveform shaper 5, a fiber isolator 6 and a polarization controller 7. The output end of the pumping light source 1 is connected to the shortwave input end of the wavelength division multiplexer 2, the output end of the wavelength division multiplexer 2 is connected to the input end of the gain fiber 3, the output end of the gain fiber 3 is connected to the input end of the first fiber coupler 4a, the large-scale output end of the first fiber coupler 4a is connected to the input end of the waveform shaper 5, the output end of the waveform shaper 5 is connected to the input end of the fiber isolator 6, the output end of the fiber isolator 6 is connected to the input end of the polarization controller 7, the output end of the polarization controller 7 is connected to the longwave input end of the wavelength division multiplexer 2, and the small-scale output end of the first fiber coupler 4a is connected to the input end of the biochemical reactor module B.

[0071] The gain fiber 3 is used to generate and amplify a laser signal in a specific operating wavelength band. The pump light source 1 is used to generate a pump laser signal, providing energy to the working material in the gain fiber 3 and stimulating its energy level transitions, thereby generating a laser signal in the operating wavelength band. The wavelength division multiplexer 2 combines the pump laser signal with the operating wavelength band laser signal output by the polarization controller and injects the combined laser signal into the gain fiber 3. The first fiber coupler 4a is used to split the laser signal into two paths: a smaller path is transmitted as the output laser signal to the biochemical reactor module B, and a larger path is returned as the echo signal to the high-repetition-rate ultrafast laser module A. The waveform shaper 5 is used to de-chirp and suppress jitter of the laser signal operating in the high-repetition-rate ultrafast laser module A, stabilizing the output laser signal in the ultrafast ground-state soliton pulse state. The fiber isolator 6 is used to guide the direction of the laser signal in the high-repetition-rate ultrafast laser module. The polarization controller 7 is used to adjust the polarization state of the high-repetition-rate ultrafast laser module.

[0072] In this embodiment of the present invention, a high-repetition-rate ultrafast laser module A comprises a pumping light source 1, a wavelength division multiplexer 2, a gain fiber 3, a first fiber coupler 4a, a waveform shaper 5, a fiber isolator 6, and a polarization controller 7. The components within the high-repetition-rate ultrafast laser module A are connected as follows: the output of the pumping light source 1 is connected to the shortwave input of the wavelength division multiplexer 2, the output of the wavelength division multiplexer 2 is connected to the input of the gain fiber 3, the output of the gain fiber 3 is connected to the input of the first fiber coupler 4a, the large-scale output of the first fiber coupler 4a is connected to the input of the waveform shaper 5, the output of the waveform shaper 5 is connected to the input of the fiber isolator 6, the output of the fiber isolator 6 is connected to the input of the polarization controller 7, the output of the polarization controller 7 is connected to the longwave input of the wavelength division multiplexer 2, and the small-scale output of the first fiber coupler 4a is connected to the input of the biochemical reactor module B. The splitting ratio range of the first fiber coupler used for the output end of the high repetition rate ultrafast laser module is between 1:99 and 45:55. The high splitting ratio output port serves as an echo port to re-input the signal into the high repetition rate ultrafast laser module, and the small splitting ratio output port serves as the total output port of the high repetition rate ultrafast laser module.

[0073] It should be noted that the embodiment of the present invention requires the pumping power of the pump light source 1 to be above 700 mW to ensure that the ultrafast laser signal can effectively transmit the reaction system in the sample window. The repetition frequency of the pulse signal output by the high repetition rate ultrafast laser module is 10 GHz. The waveform shaper 5 can completely eliminate the chirp and jitter of the laser output pulse, so that the output pulse width is fixed at 20 fs, the time-bandwidth product is maintained at 0.315, and it can operate stably in this operating state for at least 105 hours.

[0074] The biochemical reactor module B is used for fermentation reaction and is provided with a sampling window 9 to realize real-time monitoring of the reaction process;

[0075] Specifically, the biochemical reactor module B includes a fermentation tank 8 and a sampling window 9. The output end of the high repetition rate ultrafast laser module A is connected to the input end of the sampling window 9. The sampling window 9 is connected to the interior of the fermentation tank 8. The output end of the sampling window 9 is connected to the input end of the ultrafast biochemical reaction kinetics measurement module C.

[0076] Among them, the fermentation tank 8 is used to provide a container for the reaction system to carry out biochemical reactions; the sampling window 9 is used to provide a monitoring window for the reaction system, allowing the laser signal generated by the high-repetition-rate ultrafast laser module A to penetrate the reaction system and couple the transmitted light to the optical fiber end face at the output end on the other side of the sampling window, so that the signal can be transmitted to the ultrafast biochemical reaction kinetics measurement module C.

[0077] In this embodiment of the present invention, the functional component of biochemical reactor module B is a fermenter equipped with a sampling window. Fermenter 8 provides the appropriate physical and chemical environment required for fermentation. Single-mode fiber coupling is used between the low-splitting ratio output port of the first fiber coupler 4a in the high-repetition-rate ultrafast laser module and the input end of the sampling window 9, allowing the ultrafast ground-state soliton pulse to enter the reaction system within the sampling window. Assuming that the pulse sequence contains M ultrafast pulses, each with N frequency components, the complex amplitude expression Ψ for the mth ultrafast pulse is: m (t) can be expressed as:

[0078]

[0079] in, and They represent the amplitude, angular frequency and initial phase of the nth frequency component in the mth ultrafast pulse, i represents the imaginary unit, and e represents the natural base. When the ultrafast pulse is in the ground state soliton pulse state without chirp and evolution, all initial phases The values ​​of are all 0. Microstructural changes caused by biochemical reactions within the reaction system alter the motion of molecular charges. After the ultrafast pulse, acting as an electromagnetic wave, is modulated by the reaction system, the complex amplitude characteristics of its transmitted field carry information about the ultrafast reaction dynamics of the system, manifesting as amplitude and phase changes of specific frequency components within the pulse. Single-mode fiber is used to couple the sample output to the input of the second fiber coupler 4b in the ultrafast biochemical reaction dynamics measurement module C, allowing the modulated signal to enter the ultrafast biochemical reaction dynamics measurement module C for measurement.

[0080] It should be noted that the temperature of the fermentation tank can be adjusted in the range of 0-70°C and the pressure can be adjusted in the range of 0.01-0.1 MPa. The sample window is made of inorganic transparent material with a thickness of 0.5 mm on one side and a maximum light attenuation of 10 in the wavelength range of 100-2000 nm. -5 dB, the refractive index is 1.44 at a wavelength of 1550 nm, and the group velocity dispersion coefficient is 21 ps·nm -1 km -1 , the nonlinear Kerr effect coefficient is 1.3W -1 km -1 During the whole fermentation cycle measurement process, the number of ultrafast pulses transmitted through the sample window is not less than 10 17 The optical signal coupling efficiency between the pigtail at the output end of the high repetition rate ultrafast laser module and the input end of the sample window is over 99%, and the optical signal coupling efficiency between the output end of the sample window and the pigtail at the input end of the ultrafast biochemical reaction kinetics measurement module is over 99%.

[0081] The ultrafast biochemical reaction kinetics measurement module C is used to perform time stretching and spectral stretching on ultrafast pulses modulated by the biochemical reaction system, and to collect pulse transient evolution data sets in real time;

[0082] Specifically, the ultrafast biochemical reaction kinetics measurement module C includes a second optical fiber coupler 4b, a time domain stretching device 10, a spectral domain stretching device 11, a first photodetector 12a, a second photodetector 12b, and an analog-to-digital converter 13. The output end of the biochemical reactor module B is connected to the input end of the second optical fiber coupler 4b, the first output end of the second optical fiber coupler 4b is connected to the input end of the time domain stretching device 10, the output end of the time domain stretching device 10 is connected to the input end of the first photodetector 12a, the output end of the first photodetector 12a is connected to the first input end of the analog-to-digital converter 13, the second output end of the second optical fiber coupler 4b is connected to the input end of the spectral domain stretching device 11, the output end of the spectral domain stretching device 11 is connected to the input end of the second photodetector 12b, the output end of the second photodetector 12b is connected to the second input end of the analog-to-digital converter 13, and the output end of the analog-to-digital converter 13 is connected to the input end of the data processing module D.

[0083] Among them, the second optical fiber coupler 4b is used to perform spectroscopic processing on the laser signal modulated by the reaction system to generate a signal to be stretched in the time domain and a signal to be stretched in the spectral domain; the time domain stretching device 10 is used to generate a pulsed time domain stretched laser signal on the time scale of the time domain stretching laser signal; the spectral domain stretching device 11 is used to map the spectral domain information of the pulse to the time domain, and improve the spectral domain measurement resolution by stretching the time scale of the signal to generate a pulsed spectral domain stretched laser signal; the first photodetector 12a is used to perform photoelectric conversion on the pulsed time domain stretched laser signal to generate a pulsed time domain stretched analog electronic response signal; the second photodetector 12b is used to perform photoelectric conversion on the pulsed spectral domain stretched laser signal to generate a pulsed spectral domain stretched analog electronic response signal; the analog-to-digital converter 13 is used to perform analog-to-digital conversion on the pulsed time domain stretched analog electronic response signal and the pulsed spectral domain stretched analog electronic response signal, respectively, to generate a pulsed time domain stretched electronic response digital signal and a pulsed spectral domain stretched electronic response digital signal, respectively.

[0084] In the embodiment of the present invention, the time domain stretching device 10 is used to perform time domain amplification on the high repetition rate ultrafast pulse signal modulated by the reaction system, and the amplification factor is within 10 5 The minimum time resolution is less than 300 as. The spectral domain stretching module 11 is used to map the spectral domain information of the high-repetition-rate ultrafast pulse signal modulated by the reaction system into the time domain, and through time domain stretching, the minimum spectral domain resolution is reduced to less than 1 pm. The first photodetector 12a and second photodetector 12b used are commercial instruments with a photoelectric response bandwidth of 12.5 GHz. The analog-to-digital converter 13 used has a bandwidth of 15 GHz, a signal-to-noise ratio of no less than 80 dB, and a spurious-free dynamic range of no less than 90 dB.

[0085] The data processing module is used to execute the ultrafast pulse field inversion algorithm, reconstruct the ultrafast pulse field eigenstate information based on the pulse transient evolution data set, and reconstruct the ultrafast reaction kinetics of starch hydrolysis from this information through artificial intelligence algorithms;

[0086] Specifically, the data processing module includes a server 14 , an input end of the server is connected to an output end of the ultrafast biochemical reaction kinetics measurement module.

[0087] Among them, the server is used to reconstruct the ultrafast pulse field eigenstate information based on the pulse time domain stretched electron response digital signal and the pulse spectrum domain stretched electron response digital signal, and reconstruct the ultrafast biochemical reaction kinetics of starch hydrolysis.

[0088] In an embodiment of the present invention, the data processing module includes a server, the input end of the server is connected to the output end of the ultrafast biochemical reaction kinetics measurement module, and the server can convert the pulse time domain stretched electronic response digital signal and the pulse spectrum domain stretched electronic response digital signal into a pulse time domain kinetics data set and a pulse spectrum domain kinetics data set respectively, forming a pulse transient kinetics data set. The minimum single-core operating frequency of the server is 3.2GHz, the minimum number of cores is 192, the minimum memory size is 1TB, and the minimum storage space is 1PB. In the detection of the full fermentation cycle, the number of pulses in the data set is not less than 10 17 The number of sampling points in the time domain for each pulse is no less than 2000, and the number of sampling points in the spectral domain is no less than 3000. The data processing module runs the Gerchberg-Saxton iterative algorithm to invert the pulse spectral domain phase dynamics dataset from the pulse transient dynamics dataset, and then reconstruct the pulse field eigenstate dynamics dataset by combining the pulse spectral domain dynamics dataset.

[0089] It should be noted that the Gerchberg-Saxton iterative algorithm is used to invert the pulse spectral domain phase dynamics dataset. By combining the pulse spectral domain phase dynamics dataset with the pulse spectral domain dynamics dataset, the complex amplitude expression can be used to reconstruct the pulse spectral domain complex amplitude dynamics dataset, which is expressed as:

[0090]

[0091] in, and φ m (ω) represents the spectral domain complex amplitude, spectral domain amplitude, and spectral domain phase of the mth pulse in the dataset, respectively. Subsequently, the reconstructed pulse time domain dynamics dataset can be obtained by Fourier transforming the results and taking the intensity. The maximum tolerable normalized mean square error between the reconstructed pulse time domain dynamics dataset and the measured pulse time domain dynamics dataset is 10 –10 When the iterative algorithm runs until the error is lower than the maximum tolerable threshold, the iteration can be stopped, and the pulse spectrum domain phase dynamics dataset obtained by the iteration at this time can be used to reconstruct the pulse field eigenstate dynamics dataset.

[0092] Furthermore, by using an artificial intelligence algorithm, by comparing a conventional ultrafast ground state soliton pulse field eigenstate dynamics dataset with a pulse field eigenstate dynamics dataset modulated by a reaction system, an ultrafast reaction dynamics dataset of the reaction system is obtained, specifically including an ultrafast molecular weight distribution change dynamics dataset, an ultrafast chain length distribution change dynamics dataset, and an ultrafast weight average molecular weight change dynamics dataset. The neural network model in the artificial intelligence algorithm takes the ultrafast ground state soliton pulse field eigenstate dynamics dataset and the pulse field eigenstate dynamics dataset modulated by a reaction system as input, and takes the ultrafast molecular weight distribution change dynamics dataset, the ultrafast chain length distribution change dynamics dataset, and the ultrafast weight average molecular weight change dynamics dataset as output. The normalized mean square error of the training set, validation set, and test set of the neural network model should all be less than 10 –8 .

[0093] Therefore, the embodiment of the present invention can measure the ultrafast reaction kinetics of starch hydrolysis during the entire fermentation cycle. Existing methods for detecting starch hydrolysis during the entire fermentation cycle are all based on periodic sampling detection methods. This method can only obtain the microscopic structure of the material in the reaction system at the sampling time node, and cannot achieve the measurement of ultrafast biochemical reaction kinetics; existing ultrafast reaction kinetics measurement methods can only measure the reaction kinetics in a short period of time by mechanically mixing the reactants to trigger the reaction, and cannot perform continuous detection of reactions that have already started in other reaction environments. Therefore, this embodiment can break through the limitations of existing detection methods in time resolution and measurement range, and achieve accurate measurement and characterization of ultrafast reaction kinetics of starch hydrolysis with a time resolution of the order of hundreds of as and a wavelength resolution of the order of pm during the entire fermentation cycle of several months. Therefore, the embodiment of the present invention not only has important application value in the field of food fermentation science, but also shows great potential in the field of ultrafast chemical measurement.

[0094] Reference Figure 2 A method for measuring ultrafast reaction kinetics of starch hydrolysis during a full fermentation cycle comprises the following steps:

[0095] S1. Obtain high repetition rate ultrafast ground state soliton pulse signal;

[0096] It should be noted that the step of obtaining a high repetition rate ultrafast ground state soliton pulse signal specifically includes: adjusting the polarization controller and the pump light source settings to obtain a conventional high repetition rate ultrafast pulse signal with a repetition rate of 10 GHz; using a waveform shaper to eliminate pulse chirp and jitter through filtering attenuation and phase adjustment to obtain a high repetition rate ultrafast ground state soliton pulse signal with a pulse width stabilized at 20 fs and a time-bandwidth product maintained at 0.315. The output signal maintains this state for at least 10 5 h, can support stable measurement of the entire fermentation cycle.

[0097] S2, modulating the ultrafast ground state soliton pulse signal through the reaction system to obtain a modulated signal;

[0098] It should be noted that the step of modulating the ultrafast ground-state soliton pulse signal through the reaction system specifically includes inputting the ultrafast ground-state soliton pulse signal into the reaction system through the sampling window, utilizing the changes in the microscopic molecular structure of the substance caused by chemical changes in the reaction system to modulate the amplitude and phase of each frequency component in the ultrafast ground-state soliton pulse signal, so that the ultrafast pulse signal carries ultrafast biochemical reaction kinetics information, and inputting the signal into the ultrafast biochemical reaction kinetics measurement module for measurement.

[0099] S3. performing time domain stretching and spectral domain stretching on the modulated signal and performing data acquisition to obtain a pulse transient evolution dataset, wherein the pulse transient evolution dataset includes a pulse time domain dynamics dataset and a pulse spectral domain dynamics dataset;

[0100] It should be noted that the step of performing time domain stretching and spectral domain stretching processing on the modulated signal and performing data acquisition to obtain a pulse transient evolution data set specifically includes converting the original modulated pulse sequence signal into a pulse time domain envelope sequence signal and a pulse spectral domain envelope sequence signal respectively through time lens technology and dispersion Fourier transform technology, with a minimum time domain resolution of no more than 300as and a minimum spectral domain resolution of no more than 1pm; further performing continuous data acquisition to obtain a pulse time domain dynamics data set and a pulse spectral domain dynamics data set, with a continuous data acquisition time of 10 5 h or more, covering the entire fermentation cycle.

[0101] S4. Inverse processing is performed on the pulse transient evolution data set to obtain a pulse field eigenstate dynamics data set;

[0102] It should be noted that the step of inverting the pulse transient evolution data set to obtain the pulse field eigenstate dynamics data set specifically includes using the Gerchberg–Saxton algorithm to iteratively invert the pulse spectral domain phase evolution data set, substituting the inverted pulse spectral domain phase evolution data set and the pulse spectral domain evolution data set into the pulse field complex amplitude expression, reconstructing the pulse field eigenstate dynamics data set, and performing Fourier transform and modular squaring on the data to obtain the reconstructed pulse time domain evolution data set. The maximum normalized mean square error between the measurement and reconstruction results of the pulse time domain evolution data set is no more than 10 –10 .

[0103] S5. Analyze the pulsed field eigenstate kinetics dataset to obtain a starch hydrolysis ultrafast reaction kinetics dataset.

[0104] It should be noted that the step of parsing the pulsed field eigenstate kinetics dataset to obtain the ultrafast starch hydrolysis reaction kinetics dataset specifically involves using an artificial intelligence neural network model to parse the pulsed field eigenstate kinetics dataset. By inputting an ultrafast ground-state soliton pulsed field eigenstate kinetics dataset and a pulsed field eigenstate kinetics dataset modulated by the reaction system, the model can use ultrafast molecular weight distribution change kinetics datasets, ultrafast chain length distribution change kinetics datasets, and ultrafast weight-average molecular weight change kinetics datasets as prediction outputs to reveal the ultrafast reaction kinetics of starch hydrolysis during the entire fermentation cycle.

[0105] Reference Figure 3 Measurement results from a device for measuring the ultrafast reaction kinetics of starch hydrolysis over a full fermentation cycle include spectral domain macroscopic measurement results A, spectral domain transient measurement results A, time domain macroscopic measurement results B, time domain transient measurement results B, phase macroscopic inversion results C, and phase transient inversion results C. Before the 15th day, the measured reaction had not yet begun, and the measurement results remained in the soliton pulse state, demonstrating that the high-repetition-rate ultrafast laser module was operating in a stable state. Starting on the 15th day, the measured reaction began, and interference fringes appeared in the spectrum, becoming increasingly dense as the reaction progressed. The pulse profile showed splitting, with the spacing increasing as the reaction progressed. The phase showed an overall increase. Spectral domain transient measurement results A, time domain transient measurement results B, and phase transient inversion results C show the measurement results from 0 to 2 nanoseconds on day 30. This demonstrates that the present invention not only measures biochemical reactions over a timescale of up to two months but also captures changes at the nanosecond level, demonstrating its ability to measure ultrafast reaction kinetics of starch hydrolysis over a full fermentation cycle. After day 45, the reaction stopped and the measurements stabilized at a new steady state, further illustrating the stability of the system.

[0106] The advantages of the embodiments of the present invention over the prior art are:

[0107] 1) The waveform shaper ensures that the high repetition rate ultrafast laser module can stably output the detection signal throughout the entire fermentation cycle, avoiding signal distortion caused by random jitter of the detection signal.

[0108] 2) Leveraging the characteristics of the ultrafast ground-state soliton pulse—no evolution, no chirp, and an initial zero phase for all frequency components within the pulse—all phase variations can be attributed to the modulation of the reaction system. Because phase is a more microscopic feature than temporal and spectral envelopes, it is possible to characterize the details of ultrafast reaction dynamics with higher resolution.

[0109] 3) Through time-domain stretching and spectral-domain stretching techniques, the shortcomings of existing measuring instruments in sampling accuracy when measuring ultrafast events are compensated, enabling instruments with low sampling bandwidth and low sampling accuracy to achieve more complete characterization of the eigenstates of ultrafast pulse fields, thereby achieving higher-precision measurements of ultrafast reaction dynamics.

[0110] In summary, the embodiments of the present invention modulate the ultrafast reaction kinetics information of starch hydrolysis onto an ultrafast ground state soliton pulse signal, and stretch and amplify the modulated signal of the fs order in the time domain and spectral domain respectively through time domain stretching technology and spectral domain stretching technology, and then perform high-precision measurement through conventional gigahertz-level instruments; based on the Gerchberg–Saxton iterative algorithm, the inversion of the pulse field spectral domain phase data set is realized, and the pulse field eigenstate dynamics data set is constructed by combining the pulse field spectral domain data set, and the ultrafast reaction kinetics of starch hydrolysis is analyzed using an artificial intelligence neural network algorithm, including an ultrafast molecular weight distribution change kinetics data set, an ultrafast chain length distribution change kinetics data set, and an ultrafast weight average molecular weight change kinetics data set.

[0111] The contents of the above method embodiments are all applicable to the present system embodiments. The functions specifically implemented by the present system embodiments are the same as those of the above method embodiments, and the beneficial effects achieved are also the same as those achieved by the above method embodiments.

[0112] The above embodiments are preferred implementations of the present invention, but the implementations of the present invention are not limited to the above embodiments. Any other changes, modifications, substitutions, combinations, and simplifications that do not deviate from the spirit and principles of the present invention should be considered equivalent replacement methods and are included in the scope of protection of the present invention.

Claims

1. A device for measuring ultrafast reaction kinetics of starch hydrolysis during the entire fermentation cycle, characterized in that The system comprises a high repetition rate ultrafast laser module, a biochemical reactor module, an ultrafast biochemical reaction kinetics measurement module and a data processing module; the output end of the high repetition rate ultrafast laser module is connected to the input end of the biochemical reactor module, the output end of the biochemical reactor module is connected to the input end of the ultrafast biochemical reaction kinetics measurement module, and the output end of the ultrafast biochemical reaction kinetics measurement module is connected to the input end of the data processing module; The high repetition rate ultrafast laser module is used to generate high repetition rate ultrafast ground state soliton pulses; The biochemical reactor module is used for fermentation reaction and is provided with a sampling window to realize real-time monitoring of the reaction process; The ultrafast biochemical reaction kinetics measurement module is used to perform time domain stretching and spectral domain stretching on ultrafast pulses modulated by the biochemical reaction system, and to collect pulse transient evolution data sets in real time; The data processing module is used to execute an ultrafast pulse field inversion algorithm, reconstruct ultrafast pulse field eigenstate information based on a pulse transient evolution data set, and reconstruct the ultrafast reaction kinetics of starch hydrolysis from this information using an artificial intelligence algorithm.

2. The device for measuring ultrafast reaction kinetics of starch hydrolysis during the entire fermentation cycle according to claim 1, characterized in that: The high repetition rate ultrafast laser module includes a pumping light source, a wavelength division multiplexer, a gain fiber, a first fiber coupler, a waveform shaper, a fiber isolator and a polarization controller, wherein the output end of the pumping light source is connected to the short-wave input end of the wavelength division multiplexer, the output end of the wavelength division multiplexer is connected to the input end of the gain fiber, the output end of the gain fiber is connected to the input end of the first fiber coupler, the large-scale output end of the first fiber coupler is connected to the input end of the waveform shaper, the output end of the waveform shaper is connected to the input end of the fiber isolator, the output end of the fiber isolator is connected to the input end of the polarization controller, the output end of the polarization controller is connected to the long-wave input end of the wavelength division multiplexer, and the small-scale output end of the first fiber coupler is connected to the input end of the biochemical reactor module, wherein; The gain optical fiber is used to generate and amplify laser signals in a specific working band; The pumping light source is used to generate a pumping laser signal, provide energy to the working material in the gain fiber, stimulate its energy level transition, and thus generate a laser signal in the working band; The polarization controller is used to adjust the polarization state of the high repetition rate ultrafast laser module; The wavelength division multiplexer combines the pumping laser signal with the working wavelength band laser signal output by the polarization controller, and injects the combined laser into the gain optical fiber; The first fiber coupler is used to split the laser signal into two paths, wherein a smaller proportion of the path is transmitted as an output laser signal to the biochemical reactor module, and a larger proportion of the path is returned as an echo signal to the waveform shaper of the high repetition rate ultrafast laser module; The waveform shaper is used to perform chirp elimination and jitter suppression on the laser signal running in the high repetition rate ultrafast laser module, so that the output laser signal is stabilized in the ultrafast ground state soliton pulse state; The optical fiber isolator is used to guide the running direction of the laser signal in the high repetition rate ultrafast laser module.

3. The device for measuring ultrafast reaction kinetics of starch hydrolysis during the entire fermentation cycle according to claim 1, characterized in that: The biochemical reactor module includes a fermentation tank and a sampling window. The output end of the first optical fiber coupler in the high repetition rate ultrafast laser module is connected to the input end of the sampling window. The sampling window is in communication with the interior of the fermentation tank. The output end of the sampling window is connected to the input end of the ultrafast biochemical reaction kinetics measurement module. The fermentation tank is used to provide a container for the reaction system to carry out biochemical reactions; The sampling window is used to provide a monitoring window for the reaction system, allowing the laser signal generated by the high repetition rate ultrafast laser module to penetrate the reaction system and couple the transmitted light to the optical fiber end face at the output end on the other side of the sampling window, so that the signal can be transmitted to the ultrafast biochemical reaction kinetics measurement module.

4. The device for measuring ultrafast reaction kinetics of starch hydrolysis during the entire fermentation cycle according to claim 1, characterized in that: The ultrafast biochemical reaction kinetics measurement module includes a second optical fiber coupler, a time domain stretching device, a spectral domain stretching device, a first photodetector, a second photodetector, and an analog-to-digital converter. The output end of the biochemical reactor module is connected to the input end of the second optical fiber coupler, the first output end of the second optical fiber coupler is connected to the input end of the time domain stretching device, the output end of the time domain stretching device is connected to the input end of the first photodetector, the output end of the first photodetector is connected to the first input end of the analog-to-digital converter, the second output end of the second optical fiber coupler is connected to the input end of the spectral domain stretching device, the output end of the spectral domain stretching device is connected to the input end of the second photodetector, the output end of the second photodetector is connected to the second input end of the analog-to-digital converter, and the output end of the analog-to-digital converter is connected to the input end of the data processing module. The second optical fiber coupler is used to perform optical splitting processing on the laser signal modulated by the reaction system to generate a signal to be stretched in the time domain and a signal to be stretched in the spectral domain; The time domain stretching device is used to stretch the time scale of the laser signal in the time domain to generate a pulsed time domain stretched laser signal; The spectral domain stretching device is used to map the spectral domain information of the pulse to the time domain, and improve the spectral domain measurement resolution by stretching the time scale of the signal, thereby generating a pulsed spectral domain stretched laser signal; The first photodetector is used to perform photoelectric conversion on the pulse time-domain stretching laser signal to generate a pulse time-domain stretching analog electronic response signal; The second photodetector is used to perform photoelectric conversion on the pulsed spectrum domain stretching laser signal to generate a pulsed spectrum domain stretching analog electronic response signal; The analog-to-digital converter is used to perform analog-to-digital conversion on the pulse time-domain stretching analog electronic response signal and the pulse spectrum domain stretching analog electronic response signal, respectively, to generate a pulse time-domain stretching electronic response digital signal and a pulse spectrum domain stretching electronic response digital signal, respectively.

5. The device for measuring ultrafast reaction kinetics of starch hydrolysis during the entire fermentation cycle according to claim 1, characterized in that: The data processing module includes a server, the input end of the server is connected to the output end of the ultrafast biochemical reaction kinetics measurement module, wherein: The server is used to reconstruct ultrafast pulse field eigenstate information based on the pulse time domain stretched electron response digital signal and the pulse spectrum domain stretched electron response digital signal, and reconstruct the ultrafast biochemical reaction kinetics of starch hydrolysis.

6. A method for measuring ultrafast reaction kinetics of starch hydrolysis during the entire fermentation cycle, characterized in that The following steps are involved: Obtain high repetition rate ultrafast ground state soliton pulse signals; The ultrafast ground state soliton pulse signal is modulated by the reaction system to obtain a modulated signal; By performing time domain stretching processing and spectral domain stretching processing on the modulated signal and performing data acquisition, a pulse transient evolution data set is obtained, wherein the pulse transient evolution data set includes a pulse time domain dynamics data set and a pulse spectral domain dynamics data set; Inverse the pulse transient evolution data set to obtain the pulse field eigenstate dynamics data set; The pulsed field eigenstate kinetics dataset was analyzed to obtain an ultrafast reaction kinetics dataset of starch hydrolysis.

7. The method for measuring ultrafast reaction kinetics of starch hydrolysis during the entire fermentation cycle according to claim 6, wherein: The step of obtaining a high repetition rate ultrafast ground state soliton pulse signal specifically includes: By adjusting the polarization controller and pump light source settings, a conventional high repetition rate ultrafast pulse signal is obtained; A waveform shaper is used to eliminate pulse chirp and jitter to obtain a high repetition rate ultrafast ground-state soliton pulse signal.

8. The method for measuring ultrafast reaction kinetics of starch hydrolysis during the entire fermentation cycle according to claim 6, wherein: The step of modulating the ultrafast ground state soliton pulse signal by the fermentation and hydrolysis reaction system to obtain a modulated signal specifically includes: The ultrafast ground-state soliton pulse signal is emitted into the fermentation and hydrolysis reaction system through a fully transparent sample window, so that the microscopic molecular changes in the fermentation and hydrolysis system can modulate the ultrafast ground-state soliton pulse signal; The transmitted modulated signal is coupled to the optical fiber end face on the other side of the sample window, so that the modulated signal is transmitted to the ultrafast biochemical reaction kinetics measurement module.

9. The method for measuring ultrafast reaction kinetics of starch hydrolysis during the entire fermentation cycle according to claim 6, wherein: The step of performing time domain stretching processing and spectral domain stretching processing on the modulated signal and performing data acquisition to obtain a pulse transient evolution data set specifically includes: The modulated signal is subjected to distortion-free time domain stretching and distortion-free spectral domain stretching respectively using time lens technology and dispersive Fourier transform technology, until the generated pulse time domain stretching signal and pulse spectral domain stretching signal can be accurately measured by conventional photodetectors; A photodetector is used to convert the pulse time domain stretching signal and the pulse spectrum domain stretching signal into a pulse time domain stretching simulated electronic response signal and a pulse spectrum domain stretching simulated electronic response signal respectively; An analog-to-digital converter is used to convert the pulse time-domain stretching analog electronic response signal and the pulse spectrum domain stretching analog electronic response signal into a pulse time-domain stretching digital electronic response signal and a pulse spectrum domain stretching digital electronic response signal respectively; The server is used to collect the pulse time domain stretched digital electronic response signal and the pulse spectral domain stretched digital electronic response signal to obtain the pulse time domain dynamics dataset and the pulse spectral domain dynamics dataset, respectively, which constitute the pulse transient evolution dataset.

10. The method for measuring ultrafast reaction kinetics of starch hydrolysis during the entire fermentation cycle according to claim 6, characterized in that: The step of performing inversion processing on the pulse transient evolution data set to obtain the pulse field eigenstate dynamics data set specifically includes: The phase inversion algorithm is used to invert the pulse spectral domain phase dynamics dataset based on the pulse transient evolution dataset, and the pulse spectral domain dynamics dataset and the pulse spectral domain phase dynamics dataset are further combined to reconstruct the pulse field eigenstate dynamics dataset; The step of analyzing the pulsed field eigenstate kinetics dataset to obtain the starch hydrolysis ultrafast reaction kinetics dataset specifically includes: Using artificial intelligence algorithms, the ultrafast reaction kinetics dataset of starch hydrolysis was obtained by inputting the ultrafast ground state soliton pulse field eigenstate dynamics dataset before and after modulation of the reaction system into the neural network.

Images