Anti-aging and repairing composition containing camellia japonica extract and application thereof

Abstract

The present application relates to a kind of anti-aging protective composition containing camellia japonica extract and application, the liposome composition includes nuclear layer, shell layer and chitosan coating from inside to outside in turn;Wherein, the nuclear layer includes camellia japonica extract and DNA sodium;The shell layer includes lecithin, cholesterol, ceramide and enzymatic tea extract.The present application is wrapped by coating liposome encapsulation technology active substance, help active ingredient penetration skin, from promoting collagen synthesis, enhancing skin elasticity, antioxidant, repair damaged skin barrier etc. multiple dimensions Comprehensive improvement skin aging condition, realize significant anti-aging protective effect.

Description

An anti-aging and repairing composition containing Camellia chrysantha extract and its application Technical Field

[0001] This invention relates to the technical field of liposomes, specifically to an anti-aging and repairing composition containing Camellia chrysantha extract, its preparation method, and its application. Background Technology

[0002] In today's society, with the improvement of people's living standards and the enhancement of health awareness, anti-aging and repair have become one of the core demands in the skin care field. Facial skin aging is a complex and gradual process, affected by multiple factors such as natural aging, photoaging, unhealthy lifestyle, environmental pollution, and mental stress. It manifests as a series of problems such as increased wrinkles, loose skin, age spots, and decreased barrier function, which seriously affect people's appearance and mental health.

[0003] Currently, there are numerous anti-aging and repair products on the market, with lotions serving as a basic category in daily skincare. However, existing anti-aging and repair lotions still have many shortcomings. On the one hand, some product formulas lack scientific rigor and systematic approach, relying excessively on single ingredients or blindly piling on popular ingredients, making it difficult to comprehensively and effectively address skin aging issues. They may even cause secondary damage to the skin due to incompatibility or irritation between ingredients. Therefore, how to reduce product irritation and improve user comfort while ensuring anti-aging and repair effects has become a key issue that urgently needs to be addressed in current anti-aging and repair products. On the other hand, the active ingredients in skincare products often have difficulty penetrating deeply to exert their effects due to the skin barrier. Therefore, this can be addressed by developing novel liposome-enhanced permeation technology. Liposomes, due to their similarity to cell membrane structures, can smoothly pass through the gaps between keratinocytes, promoting the transdermal absorption of active ingredients and thus improving bioavailability. Simultaneously, liposomes have an encapsulation effect, protecting active ingredients and improving their stability and solubility. However, most liposomes currently on the market encapsulate single ingredients and have poor stability. Summary of the Invention

[0004] The purpose of this invention is to overcome the shortcomings of the prior art and provide an anti-aging and repairing composition containing Camellia chrysantha extract, its preparation method and application. The core layer of the liposome composition includes Camellia chrysantha extract and sodium DNA, and the shell layer includes ceramide and enzymatically hydrolyzed tea extract. By using coated liposome encapsulation technology, this invention can simultaneously promote the transdermal absorption of these active ingredients, thereby achieving better efficacy.

[0005] To achieve the above objectives, the technical solution adopted by the present invention is as follows:

[0006] In a first aspect, the present invention provides an anti-aging and repairing composition containing Camellia chrysantha extract, wherein the anti-aging and repairing composition is a liposome composition, the liposome composition comprising, from the inside out, a core layer, a shell layer, and a chitosan coating; wherein the core layer comprises Camellia chrysantha extract and sodium DNA; the shell layer comprises lecithin, cholesterol, ceramide, and enzymatically hydrolyzed tea extract; the chitosan coating comprises chitosan; and the weight ratio of Camellia chrysantha extract, sodium DNA, lecithin, cholesterol, ceramide, enzymatically hydrolyzed tea extract, and chitosan is (0.1-10):(0.001-2):(0.1-10):(0.1-5):(0.001-2):(0.001-2):(0.01-3).

[0007] The function of the raw material in the liposome shell of the present invention is as follows:

[0008] The lecithin, as the main component of liposomes, possesses both hydrophilic and lipophilic properties. It is an important component of cell membranes, replenishing skin lipids, repairing damaged stratum corneum, and strengthening the skin barrier. Cholesterol, a crucial component of the skin's natural barrier, binds to lipids in liposomes, enhancing their stability and elasticity, and simultaneously strengthening skin barrier function. Chitosan molecules modify the liposome surface, giving it a positive charge, improving stability and skin absorption / permeability. It also possesses strong water absorption, repairing the skin barrier. Ceramides, a major component of intercellular lipids in the stratum corneum, help metabolize aging cells, stimulate new cell generation, smooth wrinkles, and repair the stratum corneum, playing a vital role in maintaining skin barrier function. The enzymatic hydrolysis technology for tea extract precisely breaks down tea cell walls, releasing more tea polyphenols, making the active ingredients in the tea extract more easily absorbed by the skin, exerting antioxidant and anti-inflammatory effects. Furthermore, the encapsulation of the tea extract within the liposome shell further enhances its skin permeability and stability.

[0009] The role of the raw material in the liposome core layer of the present invention is as follows:

[0010] The flavonoids and tea polyphenols in the Camellia chrysantha extract are the main antioxidants, which have the activity of scavenging free radicals, reducing the damage of oxidative stress to the skin, delaying skin aging, enhancing skin elasticity, and reducing the formation of wrinkles. The core advantage of the sodium DNA (polydeoxyribonucleic acid, PDRN) lies in activating the skin's self-repair ability at the cellular level, while providing a gentle anti-aging solution. It is encapsulated in the liposome core layer to improve its skin permeability and anti-aging effect.

[0011] The chitosan-coated liposomes have a positively charged surface. This positively charged liposomes can interact with the negatively charged surface of the skin, altering the secondary structure of keratin in the stratum corneum, increasing cell membrane fluidity and skin permeability, making it easier for the active ingredients in skincare products to be absorbed, thus enhancing product efficacy. Secondly, the chitosan-coated liposomes can exert antibacterial and anti-inflammatory effects, reducing inflammatory symptoms and promoting skin healing. Their antibacterial mechanism mainly involves interacting with negatively charged components on the bacterial surface, disrupting the bacterial cell wall and cell membrane structure, leading to bacterial death. Furthermore, the liposomes of this invention have a core layer encapsulating Camellia chrysantha extract and sodium DNase, and a shell layer encapsulating ceramide and enzymatically hydrolyzed tea extract, which can simultaneously promote the stability and transdermal absorption of these active ingredients, thereby achieving better efficacy.

[0012] Preferably, the weight ratio of the Camellia chrysanthemi extract to sodium DNA is (1-3):(0.01-0.5).

[0013] Preferably, the weight ratio of lecithin, cholesterol, ceramide, enzymatically hydrolyzed tea extract and chitosan is (1-8):(0.5-3):(0.01-1):(0.01-0.5):(0.1-1).

[0014] Preferably, the weight ratio of the Camellia chrysantha extract to sodium DNA is (1.5-2):(0.05-0.1).

[0015] Preferably, the weight ratio of lecithin, cholesterol, ceramide, enzymatically hydrolyzed tea extract and chitosan is (4-5):(0.8-1):(0.05-0.1):(0.05-0.1):(0.4-0.5).

[0016] Preferably, the method for preparing the enzymatically hydrolyzed tea extract includes the following steps:

[0017] (1) Raw material pretreatment: Fresh, clean, and unrotten tea leaves are selected as raw materials. They are dehydrated and killed by microwave treatment. After cooling, the dried tea leaves are pulverized by a pulverizing device and sieved to obtain tea powder. The power of microwave treatment is 300-500W and the treatment time is 40-60s. The particle size of the sieve is 40-60 mesh. Microwave treatment is used to destroy the enzyme activity in the tea leaves.

[0018] (2) Enzymatic hydrolysis: Sterile deionized water is added to tea powder, and after mixing and stirring evenly, a compound enzyme is added. The mixture is hydrolyzed at 35-45℃ for 2-5 hours to allow the enzyme to fully decompose the cell walls of the tea leaves and release the effective components in the tea leaves. The hydrolyzed mixture is then subjected to enzyme inactivation treatment to obtain the hydrolysate. The amount of sterile deionized water added is 6-7 times the weight of the tea powder. The compound enzyme includes cellulase and pectinase in a weight ratio of 1:1. The amount of compound enzyme added is 0.6%-1% of the weight of the tea powder. The enzyme inactivation treatment is as follows: the hydrolyzed mixture is placed in a boiling water bath for 5 minutes to deactivate the enzyme and stop the hydrolysis reaction.

[0019] (3) Three-stage extraction: Add ethanol solution to the enzymatic hydrolysate for the first stage extraction to obtain the first extract; perform the second stage extraction on the mixture after the first stage extraction to obtain the second extract; perform the third stage extraction on the mixture after the second stage extraction to obtain the extract; wherein, the mass concentration of the ethanol solution is 45-55%, the weight ratio of the enzymatic hydrolysate to the ethanol solution is 1:(3-5), the temperature of the first stage extraction is 25-35℃ and the time is 35-45min, the temperature of the second stage extraction is 55-65℃ and the time is 35-45min, and the temperature of the third stage extraction is 75-85℃ and the time is 15-25min; through the three-stage extraction, different effective components in tea can be fully extracted, and the extraction rate can be improved;

[0020] (4) Extraction treatment: The extract after multi-stage extraction is centrifuged and filtered to further purify the effective components in the extract to obtain crude tea extract. Macroporous resin is used to adsorb and desorb the crude tea extract in sequence. The desorbed extract is then vacuum distilled to obtain tea mixture.

[0021] (5) Spray drying: The tea mixture is spray dried to obtain the enzymatically hydrolyzed tea extract in powder form; wherein the spray drying parameters are: inlet air temperature of 95-105℃, outlet air temperature of 75-85℃, and feed rate of 10-20mL / min.

[0022] In a second aspect, the present invention provides a method for preparing the anti-aging and repairing composition containing Camellia chrysantha extract as described in the first aspect, comprising the following steps:

[0023] S1. Add lecithin, cholesterol, ceramide, enzymatically hydrolyzed tea extract, and 50 ml of anhydrous ethanol to a round-bottom flask. Attach the round-bottom flask to a rotary evaporator, turn on stirring and water bath heating until the components dissolve to form a lipid solution. Evaporate the anhydrous ethanol in the lipid solution until the lipids form a thin film on the inner wall of the flask. The stirring speed is 500-600 r / min, the water bath temperature is 35-45℃, the vacuum degree for evaporating anhydrous ethanol is 0.08-0.1 MPa, and the temperature is 35-45℃. The amount of anhydrous ethanol added is 2-3 times the mass of lecithin, cholesterol, ceramide, and enzymatically hydrolyzed tea extract.

[0024] S2. Dissolve Camellia chrysantha extract and sodium DNA in water to obtain a core layer solution. Add the core layer solution to the round-bottom flask in step S1 where a thin film has been formed. Turn on the stirring and heat in a water bath until the lipid film is completely hydrated to form multilayer liposomes. Then, sonicate the multilayer liposomes to obtain a liposome suspension. The mass concentration of the core layer solution is 30-40%, the stirring speed is 500-600 r / min, and the water bath temperature is 35-45℃.

[0025] S3. Chitosan Coating: Chitosan is dissolved in a 0.1%-1% glacial acetic acid solution to prepare a 0.2%-1.0% chitosan solution. The liposome suspension is mixed with the prepared chitosan solution at a ratio of 1:0.5. The mixture is stirred at 25-30℃ and then allowed to stand to allow the chitosan to bind to the liposomes through electrostatic interaction and weak hydrophobic force, thus stabilizing the electrostatic binding. The mixture is then centrifuged to obtain chitosan-coated liposomes.

[0026] S4. Ultrafiltration: The chitosan-coated liposomes are subjected to ultrafiltration to remove unencapsulated components and free lipids, thereby obtaining the anti-aging and repairing composition containing Camellia chrysantha extract.

[0027] Preferably, in step S3 of the preparation of the anti-aging and repairing composition containing Camellia chrysantha extract, the molecular weight of the chitosan is 200-450 kDa; after magnetic stirring at 200-400 rpm for 1.5 hours at 25-30°C and then standing for 30 minutes, the mixture is centrifuged at 10000 rpm for 10 minutes to remove unbound chitosan and obtain the chitosan-coated liposomes.

[0028] Preferably, in step S4 of the preparation of the anti-aging and repairing composition containing Camellia chrysantha extract, the ultrafiltration parameters are as follows: tangential flow filtration technology is used, a flat sheet membrane is used, the membrane material is regenerated cellulose, the pore size is 50 nm, the transmembrane pressure (TMP) is 0.1-0.4 MPa, and the tangential flow rate is 1-3 m / s.

[0029] Thirdly, the present invention provides the application of the anti-aging and repairing composition containing Camellia chrysantha extract in the first aspect in the preparation of cosmetics.

[0030] Fourthly, the present invention provides an emulsion comprising the following ingredients by weight percentage: 5%-20% of the anti-aging and repairing composition containing Camellia chrysantha extract as described in the first aspect, 0.05%-0.5% thickener, 1%-10% moisturizer, 1%-6% emulsifier, 5%-15% oil, 0.5%-3% preservative, and 0.01%-0.3% pH adjuster, with the balance being deionized water.

[0031] Preferably, the thickener includes at least one of xanthan gum, carbomer, hydroxyethyl acrylate / sodium acryloyldimethyl taurate copolymer, ammonium acryloyldimethyl taurate / VP copolymer, hydroxyethyl cellulose, and cetyl alcohol.

[0032] Preferably, the moisturizer includes at least one of allantoin, sodium polyacrylate, panthenol, β-glucan, trehalose, caprylyl glycol, dipropylene glycol, sodium hyaluronate, 1,2-butanediol, glycerin, and tremella polysaccharide.

[0033] Preferably, the pH adjuster includes at least one of arginine, citric acid, NaOH, and disodium EDTA.

[0034] Preferably, the emulsifier comprises at least one of PEG-100 glyceryl stearate, glyceryl stearate citrate, cetearyl glucoside, cetyl alcohol, stearyl alcohol, cetearyl alcohol, PEG-20 methyl glucose sesquistearate, methyl glucose sesquistearate, cetyl phosphate potassium, and sodium stearoyl glutamate.

[0035] Preferably, the oil comprises at least one of caprylic / capric triglyceride, polydimethylsiloxane, jojoba oil, grape seed oil, meadowfoam seed oil, squalane, macadamia nut oil, and camellia oil.

[0036] Preferably, the preservative includes at least one selected from 1,2-hexanediol, 1,2-pentanediol, ethylhexylglycerin, p-hydroxyacetophenone, phenoxyethanol, octanoyl hydroxamic acid, and sodium benzoate.

[0037] Fifthly, the present invention provides a method for preparing the emulsion of the fourth aspect, comprising the following steps:

[0038] (1) Mix the thickener and humectant evenly, add deionized water, heat to 70-80℃, homogenize, keep warm for later use, and obtain component A;

[0039] (2) Mix the oil and emulsifier, heat to 70-80℃, stir to dissolve, keep warm for later use, and obtain component B;

[0040] (3) Heat component A to 70-80℃, add component B, homogenize, and obtain emulsion base material.

[0041] (4) Stir the emulsified base material and cool it down to 30-50°C. Add the anti-aging and repair composition containing Camellia japonica extract and continue stirring until the material is uniform. Then add preservative and pH adjuster to adjust the pH to 6.0-6.5. Stop stirring and discharge the material to obtain the emulsion.

[0042] Compared with the prior art, the beneficial effects of the present invention are as follows:

[0043] The purpose of this invention is to provide an anti-aging and repairing composition. The liposome composition comprises, from the inside out, a core layer, a shell layer, and a chitosan coating. The core layer includes Camellia chrysantha extract and sodium DNA, while the shell layer includes ceramide and enzymatically hydrolyzed tea extract. These active ingredients are absorbed transdermally through liposome encapsulation technology. This allows the liposome composition of this invention to comprehensively improve skin aging from multiple dimensions, such as promoting collagen synthesis, enhancing skin elasticity, anti-oxidation, and repairing damaged skin barriers, thereby achieving significant anti-aging and repairing effects. Attached Figure Description

[0044] Figure 1 shows a comparison of the emulsion used in Application Example 1 before and after application. Detailed Implementation

[0045] To better illustrate the purpose, technical solution, and advantages of the present invention, the present invention will be further described below in conjunction with specific embodiments.

[0046] The sources of the raw materials used in the following examples and comparative examples are as follows:

[0047] Golden Camellia Extract: Manufacturer: Ximuyuan (Xiamen) Biotechnology Co., Ltd.; Product: Golden Camellia Extract.

[0048] Calendula extract: Manufacturer: Xi'an Lvtian Biotechnology Co., Ltd., Model: PBJ0301;

[0049] Sodium DNA: Shenzhen Weiqi Technology Co., Ltd., product name: Sodium DNA;

[0050] Ceramide: Shanghai Yuanye Biotechnology Co., Ltd., model S35454-25mg;

[0051] Tea: Wuyishan Wuming Keji Tea Industry Co., Ltd., type: green tea;

[0052] Cellulase: ROHAMENT® CL, developed by British Enzyme Trading (Shanghai) Co., Ltd.

[0053] Pectinase: Ningxia Xiasheng Industrial Group Co., Ltd., model number FFY-0654.

[0054] Unless otherwise specified, all other materials and reagents used in the examples are commercially available.

[0055] The method for preparing enzymatically hydrolyzed tea extract includes the following steps:

[0056] The method for preparing the enzymatically hydrolyzed tea extract includes the following steps:

[0057] (1) Raw material pretreatment: Fresh, clean, and rot-free tea leaves are selected as raw materials. They are dehydrated and killed by microwave treatment. After cooling, the dried tea leaves are pulverized by a pulverizing device and sieved to obtain tea powder. The power of microwave treatment is 400W and the treatment time is 50s. The particle size of the sieved particles is 40-60 mesh. Microwave treatment is used to destroy the enzyme activity in the tea leaves.

[0058] (2) Enzymatic hydrolysis: Sterile deionized water is added to tea powder, and after mixing and stirring evenly, a compound enzyme is added. The mixture is hydrolyzed at 40°C for 4 hours to allow the enzyme to fully decompose the cell walls of the tea leaves and release the effective components in the tea leaves. The hydrolyzed mixture is then subjected to enzyme inactivation treatment to obtain the hydrolysate. The amount of sterile deionized water added is 7 times the weight of the tea powder. The compound enzyme includes cellulase and pectinase in a weight ratio of 1:1. The amount of compound enzyme added is 0.8% of the weight of the tea powder. The enzyme inactivation treatment is as follows: the hydrolyzed mixture is placed in a boiling water bath for 5 minutes to deactivate the enzyme and stop the hydrolysis reaction.

[0059] (3) Three-stage extraction: Add ethanol solution to the enzymatic hydrolysate for the first stage extraction to obtain the first extract; perform the second stage extraction on the mixture after the first stage extraction to obtain the second extract; perform the third stage extraction on the mixture after the second stage extraction to obtain the extract; wherein, the mass concentration of ethanol solution is 50%, the weight ratio of enzymatic hydrolysate to ethanol solution is 1:4, the first stage extraction temperature is 30℃ and the time is 40min, the second stage extraction temperature is 60℃ and the time is 40min, and the third stage extraction temperature is 80℃ and the time is 20min;

[0060] (4) Extraction treatment: The extract after multi-stage extraction is centrifuged and filtered to further purify the effective components in the extract, resulting in crude tea extract. The crude tea extract is then purified by adsorption and desorption using macroporous resin. Specifically, AB-8 type weakly polar macroporous resin is used for purification. First, the pH of the crude extract is adjusted to 3.0-4.0 and filtered. The sample is then loaded onto the pretreated resin column at a flow rate of 2-3 BV / h (the dynamic adsorption capacity of the resin is 80-100 mg tea polyphenols / mL). Adsorption is stopped when the concentration of tea polyphenols in the effluent reaches 1 / 5-1 / 10 of the sample solution. Then, the column is washed with deionized water at 3-4 BV / h until the conductivity is ≤50 μS / cm to remove macromolecular impurities. The column is then washed with 20% ethanol (2 BV, 2 BV / h), 30% ethanol (3 BV, 1.5 BV / h), and 50% ethanol (4 BV, 1.5 BV / h) in sequence. Tea extract was obtained by gradient elution with BV / h and 30% and 50% ethanol elution fractions combined. The resin can be reused after rinsing with 70% ethanol and acid-base regeneration.

[0061] (5) Spray drying: The eluent is concentrated under reduced pressure (≤50℃) to a solid content of 20%-30% and then spray dried; the parameters for spray drying are: air inlet 160-180℃, air outlet 70-90℃, and finally powdered tea extract is obtained.

[0062] Examples 1-7 and Comparative Examples 1-5

[0063] The mass ratios of the components in the anti-aging and repair compositions of Examples 1-7 and Comparative Examples 1-5 are shown in Table 1. The total mass parts of each anti-aging and repair composition are 100 parts.

[0064] The preparation methods of the anti-aging repair compositions of Examples 1-7 and Comparative Examples 1-5 include the following steps:

[0065] S1. Add lecithin, cholesterol, ceramide, enzymatically hydrolyzed tea extract, and anhydrous ethanol to a round-bottom flask. Attach the flask to a rotary evaporator, turn on stirring and water bath heating until the components dissolve, forming a lipid solution. Evaporate the anhydrous ethanol from the lipid solution until a thin film forms on the inner wall of the flask. The stirring speed is 550 r / min, the water bath temperature is 40℃, and the pressure and temperature for evaporating anhydrous ethanol are 0.09 MPa and 40℃. The amount of anhydrous ethanol added is twice the mass of lecithin, cholesterol, ceramide, and enzymatically hydrolyzed tea extract.

[0066] S2. Dissolve Camellia chrysanthemi extract and sodium DNA in water to obtain a core layer solution. Add the core layer solution to the round-bottom flask in step S1 where a thin film has been formed. Turn on the stirring and heat in a water bath until the lipid film is completely hydrated to form multilayer liposomes. Then, sonicate the multilayer liposomes to obtain a liposome suspension. The mass concentration of the core layer solution is 35%, the stirring speed is 550 r / min, the water bath temperature is 40℃, and the sonication frequency is 20 kHz, the power is 500 W, and the time is 20 min.

[0067] S3. Chitosan Coating: Select chitosan with a molecular weight of 200-450 kDa: concentration 0.2%-1.0% (soluble in 0.1%-1% glacial acetic acid), magnetically stir at 200-400 rpm for 12 hours until completely dissolved, filter to remove bacteria (0.22 μm filter membrane), mix the liposome suspension with the prepared chitosan solution at a ratio of 1:0.5, magnetically stir at 200-400 rpm for 1.5 hours at 25-30℃, then let stand for 30 minutes to stabilize the electrostatic binding, centrifuge (10,000 rpm, 10 minutes) to remove unbound chitosan, and obtain the chitosan-coated liposomes;

[0068] S4. Ultrafiltration: The chitosan-coated liposomes are subjected to ultrafiltration, specifically: tangential flow filtration technology is used, a flat sheet membrane is used, the membrane material is regenerated cellulose with a pore size range of 50 nm, the transmembrane pressure (TMP) is controlled at 0.1-0.4 MPa, and the tangential flow rate is 1-3 m / s, to remove unencapsulated components and free lipids, thereby obtaining the anti-aging and repairing composition containing Camellia chrysantha extract.

[0069] Table 1. Weight ratio of each raw material in each anti-aging and repair composition.

[0070]

[0071] Comparative Example 6

[0072] The difference between Comparative Example 6 and Example 1 is that Comparative Example 6 replaces the Camellia chrysantha extract with Calendula officinalis extract.

[0073] Comparative Example 7

[0074] The difference between Comparative Example 7 and Example 1 is that Comparative Example 7 uses tea extract instead of enzymatically hydrolyzed tea extract, and the preparation method of the tea extract includes the following steps:

[0075] (1) Raw material pretreatment: Fresh, clean, and unrotten tea leaves are selected as raw materials, and microwave treatment is used to dehydrate and kill the green. After cooling, the dried tea leaves are pulverized using a pulverizing device and sieved to obtain tea powder. The power of microwave treatment is 400W and the treatment time is 50s. The particle size of the sieved particles is 40-60 mesh.

[0076] (2) Three-stage extraction: Take the crushed tea leaves, add ethanol solution, and perform the first stage extraction to obtain the first stage extract; perform the second stage extraction on the mixture after the first stage extraction to obtain the second stage extract; perform the third stage extraction on the mixture after the second stage extraction to obtain the extract; wherein, the mass concentration of ethanol solution is 50%, the weight ratio of enzyme hydrolysate to ethanol solution is 1:4, the first stage extraction temperature is 30℃ and the time is 40min, the second stage extraction temperature is 60℃ and the time is 40min, and the third stage extraction temperature is 80℃ and the time is 20min;

[0077] (3) Extraction treatment: The extract after multi-stage extraction is centrifuged and filtered to further purify the effective components in the extract to obtain crude tea extract. Macroporous resin is used to adsorb and desorb the crude tea extract in sequence. The desorbed extract is then vacuum distilled to obtain tea mixture.

[0078] (4) Spray drying: The tea mixture is spray dried to obtain the tea extract in powder form; wherein the spray drying parameters are: inlet air temperature of 100℃, outlet air temperature of 80℃, and feed rate of 15mL / min.

[0079] Comparative Example 8

[0080] The difference between Comparative Example 8 and Example 1 is that the four components in the composition—ceramide, enzymatically hydrolyzed tea extract, sodium DNA, and Camellia chrysantha extract—were directly stirred and mixed at 500 rpm for 20 minutes to obtain the composition.

[0081] Test Example 1: Test on the reduction of inflammatory factors by anti-aging and repair compositions

[0082] I. Reagent Preparation

[0083] 1. Culture medium preparation: Prepare DMEM culture medium containing 10% (V / V) fetal bovine serum (FBS).

[0084] 2. Trypsin-EDTA solution: Aliquot the 0.25% trypsin-EDTA solution into small volumes (e.g., 5-10 mL), freeze at -20°C, and thaw quickly in a 37°C water bath before use to avoid repeated freeze-thaw cycles affecting activity.

[0085] 3. Lipopolysaccharide (LPS): Prepare a stock solution of LPS at 1 mg / mL using sterile PBS, aliquot, and store at -20°C. For experiments, dilute with culture medium to a working solution of 1 μg / mL according to group requirements, preparing fresh solution each time.

[0086] 4. Composition Sample Solution: Dissolve the compositions of Examples 1-7 and Comparative Examples 1-8 separately in DMSO to prepare a stock solution of 10 mg / mL, and store at -20°C. When using, dilute with culture medium to a working concentration of 100 μg / mL, ensuring that the final concentration of DMSO does not exceed 0.1% to eliminate its potential impact on cells.

[0087] 5. Dexamethasone: Prepare a 10 mM stock solution of dexamethasone with anhydrous ethanol and store at -20°C. For experiments, dilute with culture medium to a 1 μM working solution and prepare fresh each time.

[0088] 6. ELISA kit related reagents: According to the instructions of the selected ELISA kit for inflammatory factors such as TNF-α and IL-1α, prepare the necessary washing buffer, detection antibody, standards and other reagents, and bring them to room temperature in advance.

[0089] II. Consumables Preparation

[0090] 1. Cell culture consumables: Prepare T75 culture flasks, 96-well cell culture plates, 15mL and 50mL centrifuge tubes, pipette tips (10μL, 200μL, 1000μL), cell scrapers, etc. Ensure that all consumables are sterile products and use them as soon as possible after opening.

[0091] 2. Other consumables: Prepare 96-well plate sealing film, aluminum foil (for protecting reagents from light), ice box (for keeping samples at low temperature during operation), etc.

[0092] III. Instrument Preparation

[0093] 1. Cell culture related instruments: Turn on the CO2 incubator in advance, adjust the temperature to 37℃, CO2 concentration to 5%, and humidity to above 95%, and clean and disinfect it. Pre-cool the centrifuge to 4℃ for cell centrifugation.

[0094] 2. Testing Instruments: Preheat the ELISA reader and calibrate and test its performance according to the instruction manual to ensure the accuracy of the test results. At the same time, prepare instruments such as vortex mixers and micropipettes, and calibrate and check their functionality.

[0095] IV. Cell Culture

[0096] RAW264.7 mouse macrophages were seeded in T75 culture flasks and cultured in DMEM medium containing 10% FBS at 37°C and 5% CO2. When the cells reached the logarithmic growth phase, they were digested with 0.25% trypsin-EDTA to adjust the cell density to 2 × 10⁻⁶ cells / year. 5 Cells / mL were seeded into 96-well plates, with 100 μL of cell suspension added to each well.

[0097] V. Drug treatment and inflammation induction

[0098] (a) Group settings

[0099] Blank control group: 100 μL of cell suspension and 100 μL of culture medium were added to each well, without adding LPS and composition sample solution.

[0100] LPS group: 100 μL of cell suspension was added to each well, followed by 50 μL of culture medium and then 50 μL of 1 μg / mL LPS solution to induce an inflammatory response.

[0101] Sample group: Add 100 μL of cell suspension to each well, then add 50 μL (per well) of the sample solution of the compositions of Examples 1-7 and Comparative Examples 1-8 at 50 μg / mL, and pre-treat the 96-well plate in an incubator for 1 hour. After pre-treatment, add 50 μL of 1 μg / mL LPS solution to each well.

[0102] Positive control group: 100 μL of cell suspension was added to each well, followed by 50 μL of 1 μM dexamethasone solution. After pretreatment for 1 hour, 50 μL of 1 μg / mL LPS solution was added.

[0103] (2) Specific operations

[0104] Following the group settings, accurately add the corresponding drugs and LPS solution using a multichannel pipette to achieve a final volume of 200 μL per well. When adding the solution, avoid direct impact of the liquid on the cells; instead, gently add the solution by placing the pipette tip against the edge of the well wall.

[0105] After adding the samples, gently shake the 96-well plate to mix the solution thoroughly. Seal the 96-well plate with sealing film to prevent moisture evaporation and contamination. Incubate the treated cells in a 37°C, 5% CO2 incubator for 24 hours. During the incubation process, avoid frequently opening the incubator door to maintain a stable culture environment.

[0106] IV. Detection and Calculation of Inflammatory Factors

[0107] After 24 hours of incubation, remove the 96-well plate from the incubator and place it on an ice pack to prevent degradation of inflammatory factors. Transfer the cell culture supernatant from the 96-well plate to a new 96-well plate, avoiding aspirating cell pellets as much as possible. If further processing of the supernatant is required (e.g., centrifugation to remove cell debris), centrifuge at 1000 rpm for 5 minutes at 4°C and collect the supernatant for detection. Follow the ELISA kit instructions, placing the ELISA plate in a microplate reader. Select the corresponding detection wavelength based on the TNF-α and IL-1α inflammatory factors in the kit and measure the absorbance (OD value) of each well. Plot the standard concentration on the X-axis and the OD value on the Y-axis using four-parameter logistic regression (4-PL) or linear regression to fit the curve. Substitute the sample OD values ​​into the standard curve equation to calculate the TNF-α or IL-1α concentration (pg / mL), as shown in the following formula:

[0108] TNF-α inhibition rate (%) = (1 - TNF-α concentration in LPS group / TNF-α concentration in test sample group) × 100%;

[0109] IL-1α inhibition rate (%) = (1 - IL-1α concentration in LPS group / IL-1α concentration in test sample group) × 100%;

[0110] The test sample group includes the sample group, blank control group, and positive control group. Specific data are shown in Table 2.

[0111] Table 2. TNF-α inhibition rate and IL-1α inhibition rate of the compositions in each group.

[0112] Group / Performance TNF-α Inhibition Rate / % IL-1α Inhibition Rate / % Example 1 78.4 66.3 Example 2 76.3 64.5 Example 3 76.5 64.8 Example 4 72.2 62.2 Example 5 76.2 63.9 Example 6 69.4 61.2 Example 7 69.9 61.5 Comparative Example 1 29.7 24.5 Comparative Example 2 50.7 41.2 Comparative Example 3 57.9 49.2 Comparative Example 4 35.2 30.3 Comparative Example 5 39.9 31.7 Comparative Example 6 65.6 55.7 Comparative Example 7 63.0 54.1 Comparative Example 8 38.2 30.0 Blank Control Group 0.2 0.4 Positive Control Group 83.5 75.3 surface

[0113] As shown in Table 2, the higher the values ​​of TNF-α inhibition rate and IL-1α inhibition rate, the better the anti-inflammatory properties of the composition.

[0114] Combining the data from Example 1 and Comparative Examples 1-5, it can be seen that the TNF-α inhibition rate and IL-1α inhibition rate of Comparative Examples 1-5 were both lower than those of Example 1 to varying degrees. This indicates that the Camellia chrysanthemi extract contains flavonoids (quercetin, kaempferol), which reduce TNF-α transcription by inhibiting the NF-κB pathway, thus playing a key role in anti-inflammation. Tea leaves release small-molecule EGCG through enzymatic hydrolysis, enhancing permeability and directly inhibiting IL-1α convertase activity. Chitosan has antibacterial and anti-inflammatory effects, alleviating inflammatory symptoms. Sodium DNase accelerates tissue repair and indirectly reduces inflammatory triggering.

[0115] Based on the data from Example 1 and Comparative Examples 6 and 7, it can be seen that the active ingredients encapsulated by liposomes have a significant impact on the anti-inflammatory effect of the composition.

[0116] As can be seen from the comparison of Example 1 and Comparative Example 7, the anti-inflammatory properties of Comparative Example 7 are significantly lower than those of Example 1. This may be because the tea extract of Comparative Example 7 has not undergone enzymatic hydrolysis. The tea releases small molecule EGCG through the enzymatic hydrolysis process, which enhances permeability and directly inhibits the activity of IL-1α convertase.

[0117] Based on the data from Examples 1 and 1-7, it can be seen that when the weight ratio of Camellia chrysantha extract, sodium DNA, lecithin, cholesterol, ceramide, enzymatically hydrolyzed tea extract, and chitosan is (1.5-2):(0.05-0.1):(4-5):(0.8-1):(0.05-0.1):(0.05-0.1):(0.4-0.5), the anti-inflammatory effect of the composition is at a relatively optimal level.

[0118] Test Example 2: Composition Test for Promoting Collagen Production

[0119] I. Reagent Preparation

[0120] 1. Culture medium preparation: Prepare DMEM culture medium containing 10% (V / V) fetal bovine serum (FBS).

[0121] 2. Trypsin-EDTA solution: Aliquot the 0.25% trypsin-EDTA solution into small volumes and store at -20°C. Before use, thaw rapidly in a 37°C water bath to avoid repeated freeze-thaw cycles that may affect activity.

[0122] 3. PBS buffer: Prepare PBS buffer with pH 7.2-7.4, autoclave it, and store it at 4°C for cell washing.

[0123] 4. Cell lysis buffer: Select a suitable cell lysis buffer (such as RIPA lysis buffer containing protease inhibitors) according to experimental needs, and prepare it fresh for use to ensure lysis effect.

[0124] 5. Collagen test kit: Carefully read the kit instructions and remove the required reagents (such as standards, detection antibodies, colorimetric reagents, etc.) from the refrigerator in advance to allow them to reach room temperature.

[0125] 6. Sample Composition Solution: Dissolve the compositions of Examples 1-7 and Comparative Examples 1-8 separately in DMSO to prepare a stock solution of 10 mg / mL, and store at -20℃. During experiments, dilute with culture medium to a working concentration of 100 μg / mL, ensuring the final DMSO concentration does not exceed 0.1%.

[0126] II. Consumables Preparation

[0127] Cell culture consumables: Prepare sterile T75 culture flasks, 96-well cell culture plates, 15mL and 50mL centrifuge tubes, pipette tips (10μL, 200μL, 1000μL), cell scrapers, etc. Use as soon as possible after opening.

[0128] Other consumables: Prepare 96-well plate sealing film, ice box (to keep samples at low temperature), vortex shaker, aluminum foil (to protect reagents from light), etc.

[0129] III. Instrument Preparation

[0130] 1. Cell culture related instruments: Turn on the CO2 incubator in advance, adjust the temperature to 37℃, CO2 concentration to 5%, and humidity to above 95%, and clean and sterilize it. Pre-cool the centrifuge to 4℃ for cell centrifugation.

[0131] 2. Testing Instruments: Preheat and calibrate the ELISA reader in advance to ensure testing accuracy. Simultaneously, prepare instruments such as micropipettes and vortex mixers, and perform functional checks and calibrations.

[0132] IV. Cell Culture and Inoculation

[0133] Human fibroblasts were removed from the liquid nitrogen tank and rapidly thawed in a 37°C water bath, with constant shaking during the process. The thawed cell suspension was then seeded into T75 culture flasks and cultured in DMEM medium containing 10% FBS at 37°C and 5% CO2. Once the cells reached the logarithmic growth phase, they were digested with 0.25% trypsin-EDTA, and the cell density was adjusted to an appropriate level (e.g., 2 × 10⁶ cells / year). 5 The cells were seeded into 96-well plates, with an appropriate amount of cell suspension added to each well (100 μL per well).

[0134] V. Drug Treatment

[0135] 1. Group settings

[0136] Blank control group: 100 μL of cell suspension and 100 μL of culture medium were added to each well, without adding sample composition solution.

[0137] Sample group: Add 50 μL (per well) of the sample composition solution of Examples 1-7 and Comparative Examples 1-8 at a concentration of 100 μg / mL, and then add 50 μL of culture medium to make the final volume of each well 200 μL.

[0138] 2. Specific operations

[0139] Use a multichannel pipette to accurately add the corresponding sample solution and culture medium according to the group settings. When adding the solution, try to avoid liquid impacting the cells. You can put the pipette tip against the edge of the well wall and add the solution slowly.

[0140] After adding the samples, gently shake the 96-well plate to mix the solution thoroughly. Seal the 96-well plate with sealing film to prevent moisture evaporation and contamination. Place the treated cells in a 37°C, 5% CO2 incubator for 48 hours. During the incubation process, avoid frequently opening the incubator door to maintain a stable culture environment.

[0141] VI. Sample Collection and Collagen Detection

[0142] 1. Cell lysis

[0143] After culture, remove the 96-well plate from the incubator and place it on an ice box to prevent collagen degradation. Discard the culture medium and gently wash the cells twice with 100 μL PBS to remove residual medium. Add 50 μL of cell lysis buffer to each well and place the 96-well plate on ice for lysis for 30 minutes, gently shaking the plate every 5-10 minutes to ensure complete cell lysis. After lysis, transfer the lysis buffer to a 1.5 mL centrifuge tube and centrifuge at 12,000 rpm for 10 minutes at 4°C. Collect the supernatant, avoiding aspirating any precipitate. Transfer the supernatant to a new centrifuge tube and store at -80°C for later use, or immediately perform collagen detection.

[0144] 2. Collagen detection and result calculation

[0145] Follow the instructions for the collagen detection kit. Place the ELISA plate in a microplate reader, select an appropriate wavelength (as specified in the kit), and measure the absorbance (OD value) of each well. Plot the standard concentration on the X-axis and the OD value on the Y-axis, and fit a curve using four-parameter logistic regression (4-PL) or linear regression. Substitute the sample OD values ​​into the standard curve equation to calculate the collagen concentration (pg / mL). The specific formula is as follows:

[0146] Collagen concentration increase rate (%) = (collagen concentration) 样品组- Collagen concentration 空白对照组 Collagen concentration 空白对照组 ×100%; the results are shown in Table 3.

[0147] Table 3. Collagen increase rate of each group of samples

[0148] Group / Performance Collagen Increase Rate / % Example 1 296.0 Example 2 278.5 Example 3 280.8 Example 4 268.6 Example 5 277.3 Example 6 252.2 Example 7 221.2 Comparative Example 1 157.0 Comparative Example 2 142.5 Comparative Example 3 189.6 Comparative Example 4 172.7 Comparative Example 5 195.3 Comparative Example 6 206.2 Comparative Example 7 201.6.7 Comparative Example 8 180.6 Blank Control Group 0 surface

[0149] As shown in Table 3, the higher the collagen promotion rate, the better the repair effect of the composition.

[0150] Combining the data from Example 1 and Comparative Examples 1-5, it can be seen that the collagen promotion rate of Comparative Examples 1-5 decreased to varying degrees compared to Example 1. This indicates that sodium DNA, as a direct activator of collagen synthesis, significantly increases dermal density by stimulating fibroblast proliferation and type III collagen expression. Camellia chrysanthemi extract is rich in flavonoids (such as kaempferol), which protect collagen structure by inhibiting MMP-1 enzyme, and its synergistic effect with sodium DNA enhances collagen density. Enzymatic hydrolysis of tea extract releases small molecule EGCG (epigallocatechin gallate), which penetrates the dermis and activates collagen gene transcription. Ceramides indirectly promote collagen synthesis by strengthening the skin barrier, reducing inflammatory damage, and protecting fibroblast function.

[0151] Based on the data from Example 1 and Comparative Examples 6 and 7, it can be seen that the collagen-promoting effect of the liposome-encapsulated component composition has a significant impact.

[0152] Based on the comparison of Example 1 and Comparative Example 7, it can be seen that the anti-inflammatory properties of Comparative Example 7 are significantly lower than those of Example 1. This may be because the tea extract of Comparative Example 7 has not undergone enzymatic hydrolysis, resulting in a lower EGCG content.

[0153] Based on the data from Examples 1 and 1-7, it can be seen that when the weight ratio of Camellia chrysantha extract to sodium DNA is (1.5-2):(0.05-0.1) and the weight ratio of lecithin, cholesterol, ceramide, enzymatically hydrolyzed tea extract and chitosan is (4-5):(0.8-1):(0.05-0.1):(0.05-0.1):(0.4-0.5), the composition has a better effect on promoting collagen production.

[0154] Application Example 1-7 and Comparative Application Example 1-8

[0155] The compositions of Examples 1-7 and Comparative Examples 1-8 were added to the emulsion at a concentration of 10 wt% to obtain the emulsions of Application Examples 1-7 and Comparative Application Examples 1-8. The formulations are shown in Table 4.

[0156] The methods for preparing emulsions in Application Examples 1-7 and Comparative Application Examples 1-8 include the following steps:

[0157] (1) Mix the thickener and humectant evenly, add pure water, heat at 75°C water bath temperature for 10 minutes, then homogenize at 4000 rpm for 5 minutes, keep warm for later use, and obtain component A.

[0158] (2) Mix the oil and emulsifier, heat to 75°C, stir to dissolve evenly, keep warm for later use, and obtain component B.

[0159] (3) Heat component A to 75°C, add component B, and homogenize at 4000 rpm for 5 min to obtain emulsion base material.

[0160] (4) Stir the emulsified base material and cool it down to 50°C. Add the anti-aging and repair composition and continue stirring until the material is uniform. Then add the preservative and pH adjuster to adjust the pH to 6.5. Stop stirring and discharge the material to obtain the emulsion.

[0161] Table 3. Emulsion formulations of Application Examples 1-7 and Comparative Application Examples 1-7

[0162]

[0163] Comparative Application Example 9

[0164] In contrast, the emulsion in Application Example 9 did not contain the anti-aging and repair composition, but used an equal amount of deionized water instead of the composition, and the preparation method was the same as in Application Example 1.

[0165] Test Example 3: Human Patch Test of Emulsion

[0166] Thirty volunteers were recruited, 15 men and 15 women, aged 20-50 years. A closed patch test method was used. Equal amounts (0.5g-0.6g) of test samples (emulsions prepared in Application Examples 1-7 and Control Examples 1-9) were placed in a specific patch applicator. The patch was then applied to the volunteers' arms with hypoallergenic adhesive tape, with eight samples applied to each arm. The patches were gently pressed to ensure even application to the skin and left on for 24 hours. After 24 hours, the patch applicator was removed, and skin reactions were observed at 0.5 hours, 24 hours, and 48 hours, and the results were recorded. The severity of adverse skin reactions is shown in Table 4 below.

[0167] Table 4. Adverse skin reaction grades

[0168] Skin reaction rating scale: 0 Negative reaction; 1 Suspicious reaction, only slight erythema; 2 Weak positive reaction (erythema reaction): erythema, infiltration, edema, papules may be present; 3 Strong positive reaction (herpes reaction): erythema, infiltration, edema, papules; reaction may extend beyond the test area; 4 Very strong positive reaction (confluent herpes reaction): obvious erythema, severe infiltration, edema, confluent herpes; reaction extends beyond the test area. surface

[0169] After testing, the lotions provided in Application Examples 1-7 and Comparative Application Examples 1-9 all showed negative reactions after human patch testing, indicating that they are safe and non-irritating to human skin.

[0170] Test Example 4: Test on the anti-wrinkle, firming, soothing, and repairing effects on the human body

[0171] 1. Experimental basis

[0172] The human efficacy evaluation test method shall be followed in accordance with the "Technical Specifications for Cosmetic Safety" (2015 edition).

[0173] 2. Subject selection

[0174] Inclusion criteria: We are recruiting Asian adult female volunteers aged 45-60 who meet the following conditions:

[0175] a) Clinical assessment indicates skin laxity (grades I-III according to clinical grading standards);

[0176] b) A self-reported history of skin allergies (confirmed by a dermatologist);

[0177] c) No history of serious systemic diseases or skin diseases.

[0178] Exclusion criteria: pregnant / lactating women, individuals with severe allergies, and individuals who have participated in other clinical trials within the past 3 months.

[0179] Number of participants: A total of 90 qualified volunteers were included and divided into 16 groups of 5 people each using a random number table.

[0180] 3. Sample application method

[0181] Test samples: Emulsions prepared in Application Examples 1-7 and Comparative Application Examples 1-9 (double-blind method numbering), wherein the serum prepared in Comparative Application Example 9 served as the blank control group, and the other application examples and comparative application examples served as the sample groups.

[0182] How to use: After cleansing in the morning and evening, volunteers should take 1mL of the sample and apply it evenly to the entire face, gently massaging until fully absorbed.

[0183] 4. Testing Cycle and Process

[0184] Test periods: 14, 28, and 56 days (D0, D...) 14 D 28 and D 56 ).

[0185] Visit time points: D0 (baseline period), D 14 D 28 and D 56 (At the end of the test) Instrument testing will be conducted.

[0186] Preparation before testing:

[0187] a) After the participants arrived, they used a uniform, non-irritating facial cleanser to clean their faces.

[0188] b) Rest for 30 minutes in a constant temperature and humidity environment (temperature 21±1℃, humidity 50±10%);

[0189] c) Keep your eyes closed and relax during the test to avoid facial expressions and movements that may interfere with the process.

[0190] The facial skin condition was tested on days 14, 28, and 56 after product use to comprehensively evaluate the product's firming and anti-wrinkle effects. Specific experimental parameters and equipment are shown in Table 5, and evaluation parameters are shown in Table 6.

[0191] Result determination: If the R2 / R7 / Q1, Wrinkle Area, and Wrinkle Length values ​​of the test area increase significantly after product use, it indicates that the test sample has a firming and anti-wrinkle effect; conversely, if the parameters do not increase and do not show significant differences, it indicates that the test product does not have a firming and anti-wrinkle effect.

[0192] Table 5 Test Parameters and Equipment

[0193]

[0194] Table 6 Evaluation Parameters

[0195] Evaluation Parameters and Their Meanings: R² Skin Elasticity: The R² value characterizes the improvement of facial skin elasticity; a higher elasticity value indicates better skin elasticity. Wrinkle Area Value: The Wrinkle Area value represents the area of ​​wrinkles; a lower Wrinkle Area value indicates a smaller area of ​​crow's feet, under-eye wrinkles, and nasolabial folds. Wrinkle Length Value: The Wrinkle Length value represents the length of wrinkles; a lower Wrinkle Length value indicates a shorter length of crow's feet, under-eye wrinkles, and nasolabial folds. surface

[0196] The formulas for calculating the evaluation parameters are as follows:

[0197] (T14, 28, 56) R² improvement rate = (R²) T0 -R2 Tn ) / R2 T0 ×100%; where, T n =T 14 T 28 T 56 .

[0198] (T14, 28, 56) Wrinkle Area value improvement rate = (Wrinkle Area) T0 -Wrinkle Area Tn Wrinkle Area T0 ×100%; where, T n =T 14 T 28 T 56 .

[0199] (T14, 28, 56) Wrinkle Length improvement rate = (Wrinkle Length) / (T14, 28, 56) T0 Wrinkle Length Tn Wrinkle Length T0 ×100%; where, T n =T 14 T 28 T 56 The data is shown in Table 7.

[0200] Table 7 Test results of each emulsion group after use

[0201]

[0202] As shown in Table 7, combining the data from Application Example 1 and Comparative Application Examples 1-5, it can be seen that the liposomes whose main components are Camellia chrysantha extract, sodium DNA, ceramide, enzymatically hydrolyzed tea extract, and chitosan can synergistically enhance the anti-wrinkle and elasticity-enhancing effects of the emulsion.

[0203] Combining the data from Application Example 1 and Comparative Application Example 6, it can be seen that the performance of the emulsion is significantly lower than that of Application Example 1. This may be because the anti-aging effect of Calendula extract is not as good as that of Camellia chrysantha extract.

[0204] Combining the data from Application Example 1 and Comparative Application Example 7, it can be seen that the performance of the emulsion is lower than that of Application Example 1. This may be because the enzymatic hydrolysis technology of tea extract precisely decomposes the cell walls of tea leaves, releasing more tea polyphenols, making the active ingredients in the tea extract easier to be absorbed by the skin and exert antioxidant and anti-inflammatory effects.

[0205] Combining the data from Application Example 1 and Comparative Application Example 8, it can be seen that Comparative Application Example 8 directly mixes the raw materials of the composition without using liposomes as a carrier for the ingredients. Its emulsion performance is significantly lower than that of Application Example 1. This may be because the form of liposomes can promote the transdermal absorption of active ingredients, and the use of coated liposome encapsulation technology to encapsulate active substances can better help the active substances penetrate the skin and achieve significant anti-aging and repair effects.

[0206] In summary, the anti-wrinkle, repairing, and elasticity-enhancing effects of the lotion are mainly based on the addition of an anti-aging and repairing composition. As shown in Figure 1, after using the lotion of Application Example 1 for 56 days, the crow's feet, under-eye wrinkles, and nasolabial folds all showed varying degrees of reduction. This may be because the anti-aging and repairing composition of the present invention can comprehensively improve the skin's aging condition from multiple dimensions, such as promoting collagen synthesis, enhancing skin elasticity, anti-oxidation, and repairing the damaged skin barrier.

[0207] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention and are not intended to limit the scope of protection of the present invention. Although the present invention has been described in detail with reference to preferred embodiments, those skilled in the art should understand that modifications or equivalent substitutions can be made to the technical solutions of the present invention without departing from the essence and scope of the technical solutions of the present invention.

Claims

1. An anti-aging and repairing composition containing Camellia chrysantha extract, characterized in that, The anti-aging and repairing composition is a liposome composition, which consists of a core layer, a shell layer and a chitosan coating from the inside out; wherein, the core layer is composed of Camellia chrysantha extract and sodium DNA; the shell layer is composed of lecithin, cholesterol, ceramide and enzymatically hydrolyzed tea extract; the raw material of the chitosan coating is chitosan; the weight ratio of Camellia chrysantha extract, sodium DNA, lecithin, cholesterol, ceramide, enzymatically hydrolyzed tea extract and chitosan is (0.1-10):(0.001-2):(0.1-10):(0.1-5):(0.001-2):(0.001-2):(0.01-3); the preparation method of the enzymatically hydrolyzed tea extract includes the following steps: (1) Raw material pretreatment: Fresh, clean, and unrotten tea leaves are selected as raw materials, microwave treatment is used for dehydration and blanching, and after cooling, the dried tea leaves are pulverized using a pulverizing device and sieved to obtain tea leaves. Powder; wherein, the power of microwave treatment is 300-500W, the treatment time is 40-60s; the particle size of sieve is 40-60 mesh; (2) Enzymatic hydrolysis treatment: add sterile deionized water to tea powder, mix and stir evenly, add compound enzyme, and enzymatically hydrolyze at 35-45℃ for 2-5h, and perform enzyme inactivation treatment on the mixed liquid after enzymatic hydrolysis to obtain enzymatic hydrolysate; wherein, the amount of sterile deionized water added is 6-7 times the weight of tea powder, the compound enzyme is cellulase and pectinase with a weight ratio of 1:1, and the amount of compound enzyme added is 0.6%-1%; (3) Three-stage extraction: Add ethanol solution to the enzymatic hydrolysate for the first stage extraction to obtain the first stage extract; perform the second stage extraction on the mixture after the first stage extraction to obtain the second stage extract; perform the third stage extraction on the mixture after the second stage extraction to obtain the extract; wherein, the mass concentration of ethanol solution is 45-55%, the weight ratio of enzymatic hydrolysate to ethanol solution is 1:(3-5), the first stage extraction temperature is 25-35℃ and the time is 35-45min, the second stage extraction temperature is 55-65℃ and the time is 35-45min, and the third stage extraction temperature is 75-85℃ and the time is 15min. -25min; (4) Extract treatment: Centrifuge and filter the extract after multi-stage extraction to obtain crude tea extract. Adsorb and desorb the crude tea extract sequentially using macroporous resin. Vacuum distill the desorbed extract to obtain tea mixture; (5) Spray drying: Spray dry the tea mixture to obtain powdered enzymatically hydrolyzed tea extract; The preparation method of the anti-aging and repair composition containing golden camellia extract includes the following steps: S1, take lecithin, cholesterol, ceramide, enzymatically hydrolyzed tea extract and anhydrous ethanol into a round-bottom flask, and install the round-bottom flask into a rotary distillation unit. On the apparatus, stir and heat in a water bath until the components dissolve to form a lipid solution; evaporate the anhydrous ethanol in the lipid solution until a thin film forms on the inner wall of the flask; the amount of anhydrous ethanol added is 2-3 times the mass of lecithin, cholesterol, ceramide, and enzymatically hydrolyzed tea extract; S2, dissolve the golden camellia extract and sodium DNase in water to obtain a core layer solution, add the core layer solution to the round-bottom flask in step S1 where a thin film has formed, stir and heat in a water bath until the lipid film is completely hydrated to form multilayer liposomes, and then sonicate the multilayer liposomes to obtain a liposome suspension; wherein, the mass concentration of the core layer solution is 30-40%. S3, Chitosan Coating: Chitosan is dissolved in a 0.1%-1% glacial acetic acid solution to prepare a 0.2%-1.0% chitosan solution. The liposome suspension is mixed with the prepared chitosan solution at a ratio of 1:0.5, stirred at 25-30℃, and then allowed to stand to stabilize the electrostatic binding. Centrifugation is then performed to obtain chitosan-coated liposomes. S4, Ultrafiltration: The chitosan-coated liposomes are subjected to ultrafiltration to remove unencapsulated components and free lipids, yielding the anti-aging and repair composition containing Camellia chrysantha extract.

2. The anti-aging and repairing composition containing Camellia chrysantha extract as described in claim 1, characterized in that, The weight ratio of the Camellia chrysanthemi extract to sodium DNA is (1.0-3.0):(0.01-0.5).

3. The anti-aging and repairing composition containing Camellia chrysantha extract as described in claim 1, characterized in that, The weight ratio of lecithin, cholesterol, ceramide, enzymatically hydrolyzed tea extract and chitosan is (1-8):(0.5-3):(0.01-1):(0.01-0.5):(0.1-1).

4. The anti-aging and repairing composition containing Camellia chrysantha extract as described in claim 1, characterized in that, The weight ratio of the Camellia chrysanthemi extract to sodium DNA is (1.5-2):(0.05-0.1).

5. The anti-aging and repairing composition containing Camellia chrysantha extract as described in claim 1, characterized in that, The weight ratio of lecithin, cholesterol, ceramide, enzymatically hydrolyzed tea extract and chitosan is (4-5):(0.8-1):(0.05-0.1):(0.05-0.1):(0.4-0.5).

6. The use of the anti-aging and repairing composition containing Camellia chrysantha extract according to any one of claims 1-5 in the preparation of cosmetics.

7. An emulsion, characterized in that, The ingredients comprise the following ingredients by weight percentage: 5%-20% of the anti-aging and repairing composition containing Camellia chrysantha extract as described in any one of claims 1-5, 0.05%-0.5% thickener, 1%-10% moisturizer, 1%-6% emulsifier, 5%-15% oil, 0.5%-3% preservative, and 0.01%-0.3% pH adjuster, with the balance being deionized water.

8. The method for preparing the emulsion according to claim 7, characterized in that, The process includes the following steps: (1) Mix the thickener and moisturizer evenly, add deionized water, heat to 70-80℃, homogenize, keep warm for later use, and obtain component A; (2) Mix the oil and emulsifier, heat to 70-80℃, stir to dissolve, keep warm for later use, and obtain component B; (3) Heat component A to 70-80℃, add component B, homogenize, and obtain emulsion base material; (4) Stir the emulsion base material and cool it down to 30-50℃, add the anti-aging and repair composition containing golden camellia extract, continue stirring until the material is uniform, then add preservative and pH adjuster to adjust the pH to 6.0-6.5, stop stirring, discharge the material, and obtain the emulsion.

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