Fluorescence excitation module and sample imaging analysis device

Abstract

The invention belongs to the technical field of fluorescence detection, and particularly relates to a fluorescence excitation module and a sample imaging analysis device.The fluorescence excitation module is applied to the sample imaging analysis device.The fluorescence excitation module comprises a plurality of excitation units which are sequentially in light path communication in the transmission direction of a light path, the plurality of excitation units are respectively used for emitting visible light with different wavelengths; the focusing lens is used for focusing the visible light emitted from the plurality of excitation units and transmitting the visible light to the transmission optical fiber, and the transmission optical fiber is provided with a synthesis end for receiving the plurality of visible light and a plurality of beam splitting ends for irradiating a sample. The plurality of excitation units of the fluorescence excitation module are integrally arranged, and the transmission optical fiber is adopted to transmit an optical path, so that the size is obviously reduced, and the miniaturization design of the fluorescence excitation module is facilitated.

Description

Technical Field

[0001] This invention belongs to the field of fluorescence detection technology, specifically relating to a fluorescence excitation module and a sample imaging analysis device. Background Technology

[0002] Fluorescence produced by fluorescence imaging technology is a photoluminescence phenomenon. High-energy excitation light excites fluorescent molecules, activating them into an excited state. Excited fluorescent molecules are unstable; their energy is rapidly released, either as heat or as light. The phenomenon of deactivation via light is called fluorescence. This controllable method of photoluminescence gives us the ability to selectively observe cells, much like a switch: turning it on causes cells to selectively emit fluorescence, and turning it off extinguishes the fluorescence. Light acts as the trigger for this switch, avoiding direct microscopic contact with cells, making it a non-invasive triggering method.

[0003] Existing fluorescence imaging analysis equipment typically uses fixed-focus lenses paired with high-power LEDs for excitation illumination. These devices are relatively large, occupying a significant amount of space, and often exhibit uneven energy distribution in the illumination spot. This also hinders the design of lightweight and miniaturized instruments. Furthermore, high-power LEDs usually require multiple optical transmission channels and multiple sets of transmission lenses to excite the sample and generate fluorescence, further increasing the size of the fluorescence imaging equipment. Summary of the Invention

[0004] The purpose of this invention is to provide a fluorescence imaging analysis device that achieves miniaturization of the fluorescence imaging analysis device.

[0005] To achieve the above objectives, the present invention provides a fluorescence excitation module for use in a sample imaging analysis device. The fluorescence excitation module includes components sequentially connected along the transmission direction of the optical path:

[0006] The excitation unit is multiple and integrated into one unit, and each excitation unit is used to emit visible light of different wavelengths;

[0007] A focusing lens and a transmission fiber are used to focus visible light emitted from multiple excitation units and transmit it to the transmission fiber, which has a combining end for receiving multiple visible light beams and multiple split ends for illuminating the sample.

[0008] In some embodiments, the excitation unit includes a light source, a collimating lens, a filter, and a dichroic mirror that are sequentially connected along the transmission direction of the optical path. The light source emits visible light within a preset wavelength range. The visible light emitted by the light source can pass through the dichroic mirror or be reflected on the dichroic mirror until the visible light is transmitted into the focusing lens.

[0009] In some implementations, the ports of multiple beam splitters are provided with collimation components for collimating visible light.

[0010] In some implementations, the distance range between the beam splitter and the center point of the sample must satisfy the following condition:

[0011]

[0012] Where R is the distance between the splitter and the center point of the sample, D is the circular diameter of the sample, and NA1 is the numerical aperture of the transmission fiber.

[0013] A second aspect of the present invention provides a sample imaging analysis apparatus, comprising:

[0014] The fluorescence excitation module described above;

[0015] An imaging lens is used to capture the fluorescence emitted by a sample and obtain a fluorescence image;

[0016] The image analysis module and the sample are located on opposite sides of the imaging lens. The image analysis module is used to receive and analyze fluorescence images.

[0017] In some embodiments, the image analysis module includes: a display unit for receiving and displaying a fluorescence image; and an analysis unit for analyzing and processing the fluorescence image, the analysis unit being located between the display unit and the imaging lens.

[0018] In some embodiments, the imaging lens includes a first lens, a second lens, an aperture, a third lens, and a fourth lens in sequence along the optical path transmission direction. The first lens and the fourth lens are placed in mirror image symmetry, the second lens and the third lens are placed in mirror image symmetry, the first lens and the fourth lens are both positive power lenses, and the second lens and the third lens are both negative power lenses.

[0019] In some embodiments, the first lens, the second lens, the third lens, and the fourth lens all satisfy the following conditions:

[0020] arctan(HI / EFL)≤8 0

[0021] Where HI is the half-image height of the image plane, and EFL is the effective focal length of the imaging lens (20);

[0022] Both the first lens and the fourth lens satisfy the following conditions:

[0023] 0.6≤|F S1 / EFL|≤1

[0024] Among them, F S1 The focal lengths of the first and fourth lenses;

[0025] Both the second and third lenses satisfy the following conditions:

[0026] 1.2≤|F S2 / EFL|≤1.6

[0027] Among them, F S2 This refers to the focal lengths of the second and third lenses.

[0028] In some embodiments, the first lens, the second lens, the third lens, and the fourth lens are all cemented lenses.

[0029] In some implementations, a filter module is also provided between the imaging lens and the image analysis module, and the filter range of the filter module is adjustable.

[0030] The aforementioned fluorescence excitation module includes an excitation unit, a focusing lens, and a transmission fiber, all connected sequentially along the optical path transmission direction. Multiple excitation units are integrated into a single unit, reducing the overall size of the fluorescence excitation module and facilitating miniaturization of the sample imaging analysis device. Furthermore, the small size of the transmission fiber allows for further reduction in the overall size of the sample imaging analysis device. Multiple beam splitters of the transmission fiber can simultaneously illuminate the sample, enhancing the fluorescence excitation effect.

[0031] Other features and advantages of the embodiments of the present invention will be described in detail in the following detailed description section. Attached Figure Description

[0032] The accompanying drawings are provided to further illustrate embodiments of the present invention and form part of the specification. They are used together with the following detailed description to explain the embodiments of the present invention, but do not constitute a limitation thereof. Those skilled in the art can obtain other drawings based on the structures shown in these drawings without any inventive effort. In the drawings:

[0033] Figure 1 This is a schematic diagram of the structure of a fluorescence excitation module provided according to an embodiment of the present invention;

[0034] Figure 2 This is a schematic diagram of the sample imaging analysis device provided according to an embodiment of the present invention;

[0035] Figure 3 This is a schematic diagram of the structure of a fluorescence excitation module according to another embodiment of the present invention;

[0036] Figure 4 This is a schematic diagram showing the light spot shining on the sample.

[0037] Figure 5This is a schematic diagram of fluorescence of different wavelengths passing through an imaging lens according to an embodiment of the present invention.

[0038] Figure 6 The MTF curve of lens imaging provided according to an embodiment of the present invention;

[0039] Figure 7 This is a lens imaging point diagram provided according to an embodiment of the present invention;

[0040] Figure 8 This is a field curvature / distortion curve under visible light detected according to an embodiment of the present invention.

[0041] Explanation of reference numerals in the attached figures

[0042] 10. Fluorescence Excitation Module

[0043] 11 Excitation Unit

[0044] 111 Light Source

[0045] 112 Collimating Lens

[0046] 113 Filter

[0047] 114 Dichroic mirror

[0048] 12 Focusing Lenses

[0049] 13. Transmission fiber optic cable

[0050] 131 Synthesis End

[0051] 132 beam splitter

[0052] 20 Imaging Lenses

[0053] 21 First Lens

[0054] 22 Second Lens

[0055] 23 Third Lens

[0056] 24. Fourth Lens

[0057] 30 Image Analysis Module

[0058] 40 samples

[0059] 50 Collimation Components

[0060] 60 filter modules Detailed Implementation

[0061] The specific embodiments of the present invention will be described in detail below with reference to the accompanying drawings. It should be understood that the specific embodiments described herein are for illustration and explanation only and are not intended to limit the present invention.

[0062] The fluorescence excitation module 10 and sample imaging analysis apparatus according to the present invention are described below with reference to the accompanying drawings.

[0063] like Figure 1 As shown, this is a schematic diagram of the structure of the fluorescence excitation module 10 provided according to an embodiment of the present invention. Figure 2 The diagram shown is a schematic representation of the sample imaging analysis device provided according to an embodiment of the present invention.

[0064] The fluorescence excitation module 10 provided in this embodiment of the invention is applied to, for example... Figure 2 In the sample imaging analysis device shown, the fluorescence excitation module 10 includes the following components sequentially connected along the optical path transmission direction:

[0065] The excitation unit 11 is multiple and integrated into one unit, and the multiple excitation units 11 are used to emit visible light of different wavelengths;

[0066] A focusing lens 12 and a transmission fiber 13 are provided. The focusing lens 12 is used to focus visible light emitted from multiple excitation units 11 and transmit it to the transmission fiber 13. The transmission fiber 13 has a combining end 131 for receiving multiple visible light beams and multiple split ends 132 for illuminating the sample 40.

[0067] Compared to using high-power LEDs to illuminate the sample 40, the integrated excitation unit 11 of this application occupies less space, and different excitation modules are used for different channels to illuminate, making the spatial light field distribution more uniform and conducive to obtaining better imaging results.

[0068] The focusing lens 12 focuses the visible light generated by the fluorescence excitation module 10 and transmits the focused light to the combining end 131 of the transmission fiber 13. The transmission fiber 13 further transmits the focused visible light to the sample 40. The transmission fiber 13 includes multiple beam-splitting ends 132, each of which is positioned towards the sample 40 to excite the sample 40 and induce fluorescence. The multiple beam-splitting ends 132 can irradiate the sample 40 from different directions, resulting in better irradiation. Furthermore, this application uses the transmission fiber 13 to transmit visible light, which occupies less space than a transmission channel formed by a mirror or lens, facilitating the miniaturization design of the fluorescence excitation module 10.

[0069] In one embodiment, such as Figure 1As shown, the excitation unit 11 includes a light source 111, a collimating lens 112, a filter 113, and a dichroic mirror 114, which are sequentially connected along the optical path transmission direction. The light source 111 emits visible light within a preset wavelength range. The visible light emitted by the light source 111 can pass through the dichroic mirror 114 or be reflected on the dichroic mirror 114 until the visible light is transmitted to the focusing lens 12. The excitation unit 11 provided in this embodiment includes a light source 111, a collimating lens 112, a filter 113, and a dichroic mirror 114, which are sequentially connected along the optical path. The light source 111 can emit visible light within a preset wavelength range, wherein the wavelength range of visible light emitted by the light source 111 of different excitation units 11 is different. The collimating lens 112 can collimate the discrete visible light emitted by the light source 111, converting the visible light passing through the collimating lens 112 into parallel-transmitting visible light. The parallel-transmitting visible light is further connected to the filter 113 for filtering, reducing stray light influence. The dichroic mirror 114 allows visible light of a specified wavelength to pass through, while visible light outside the specified range is reflected when it strikes the dichroic mirror 114. Based on this principle of the dichroic mirror 114, this invention enables filtered visible light to pass through the dichroic mirror and further into the focusing lens 12.

[0070] In one embodiment, such as Figure 1 As shown, the angle between the dichroic mirror 114 and the filter 113 is 45 degrees. When visible light passes perpendicularly through the leftmost filter 113, it will illuminate the dichroic mirror 114 and propagate among multiple dichroic mirrors 114 until it reaches the rightmost dichroic mirror 114. The light path is reflected on the rightmost dichroic mirror 114 and then transmitted to the focusing lens 12.

[0071] In one embodiment, the transmission fiber 13 has a Y-shaped structure with two split ends 132. The Y-shaped transmission fiber 13 includes two split ends 132, which can be respectively disposed on both sides of the sample 40. The ports of the split ends 132 are oriented towards the sample 40, which can make the light path illumination more uniform and the imaging effect better.

[0072] In one embodiment, such as Figure 3 The diagram shown is a schematic representation of a fluorescence excitation module according to another embodiment of the present invention. Each of the ports of the plurality of beam splitters 132 is provided with a collimation component 50 for collimating visible light. In a specific embodiment, the collimation component 50 includes a base and a collimating convex lens. Using the aforementioned collimation component 50, visible light emitted from the nozzles of the beam splitters can be collimated, compressing the divergence angle of the irradiation beam, resulting in more concentrated visible light irradiation of the sample 40, thereby more effectively exciting the fluorescence of the sample 40.

[0073] In one embodiment, the distance range between the beam splitter 132 and the center point of the sample 40 must satisfy the following condition:

[0074]

[0075] Where R is the distance between the splitter 132 and the center point of the sample 40, D is the circular diameter of the sample 40, and NA1 is the numerical aperture of the transmission fiber 13.

[0076] Since the beam splitter 132 of the transmission fiber 13 obliquely illuminates the sample 40, the shape of the light spot is elliptical. Therefore, the optimal state is for the two light spots to overlap, such as... Figure 4 The diagram shows a schematic of the light spot illuminating the sample 40. The two light spots can compensate for each other, improving the uniformity of the light spot. To effectively utilize the output optical path of the transmission fiber 13, the positional relationship between the transmission fiber 13 and the sample 40 should satisfy Rtan(θ / 2)≥D / 2, where θ is the exit cone angle of the transmission fiber 13 and D is the circular diameter of the sample 40. This results in better excitation efficiency of the optical path. The numerical aperture of the transmission fiber 13 is NA1, satisfying the relationship sin(θ / 2)≤NA1. From the above, we can obtain... Given that θ is relatively small and cos(θ / 2)≤1, it can be seen after processing that the installation distance of transmission fiber 13 should meet the following requirements.

[0077] In one embodiment, the angle between the light path emitted from the beam splitter 132 and the plane where the sample 40 is located is 45°. The beam splitter 132 set at 45° can better excite the fluorescence of the sample 40 and acquire fluorescence imaging.

[0078] In one embodiment, in order for all the light transmitted by the focusing lens 12 to be transmitted into the transmission fiber 13, the numerical aperture NA2 of the focusing lens 12 and the numerical aperture NA1 of the transmission fiber 13 should satisfy the following conditions:

[0079] NA2≤NA1

[0080] The aforementioned fluorescence excitation module 10 integrates multiple excitation units 11 into a single unit. These excitation units 11 emit visible light of different wavelengths. The visible light passes through a focusing lens 12 and enters the transmission fiber 13 from the combining end 131. The transmission fiber 13 transmits the visible light and outputs it from the beam splitting end 132. This method reduces the volume occupied by the fluorescence excitation module 10, facilitating its miniaturization. Furthermore, the excitation unit 11 includes a light source 111, a collimating lens 112, a filter 113, and a dichroic mirror 114. The collimating lens 112 and filter 113 collimate and filter the visible light emitted by the light source 111, while the dichroic mirror 114 transmits the visible light emitted by the multiple light sources 111 within the excitation unit 11, reducing its volume and eliminating stray light.

[0081] In one embodiment, a sample imaging analysis device is provided, comprising: the fluorescence excitation module 10 described above; an imaging lens 20 for acquiring the fluorescence emitted by the sample 40 and obtaining a fluorescence image; and an image analysis module 30, with the image analysis module 30 and the sample 40 located on opposite sides of the imaging lens 20, the image analysis module 30 being used to receive, analyze, and display the fluorescence image. The imaging lens 20 can acquire the fluorescence emitted by the sample 40 and perform imaging processing to obtain a fluorescence image, while the image analysis module 30 can acquire and analyze the fluorescence image transmitted by the imaging lens 20, facilitating operator observation and detection of the image.

[0082] In one embodiment, the image analysis module 30 includes: a display unit for receiving and displaying a fluorescence image; and an analysis unit for analyzing and processing the fluorescence image, located between the display unit and the imaging lens 20. The analysis unit of the image analysis module 30 can first analyze the acquired fluorescence image and then display it on the display unit. The analysis unit can analyze and process the fluorescence image to obtain the statistical results of fluorescence image pixels and the abundance of a specific target. In a specific embodiment, the image detector includes a binary pixel image sensor such as CCD or CMOS and a display. The sample 40 and the image analysis module 30 have an object-image conjugate relationship. The distance between the sample 40 and the imaging lens 20 is a first distance, and the distance between the image analysis module 30 and the imaging lens 20 is a second distance. The absolute value of the difference between the second distance and the first distance is less than a preset distance. The size of the imaging image can be adjusted by adjusting the size of the first distance and the second distance.

[0083] In one embodiment, the imaging lens 20 includes, sequentially along the optical path transmission direction, a first lens 21, a second lens 22, an aperture stop (not shown), a third lens 23, and a fourth lens 24. The first lens 21 and the fourth lens 24 are mirror-symmetrically placed, as are the second lens 22 and the third lens 23. The first lens 21 and the fourth lens 24 are both positive power lenses, while the second lens 22 and the third lens 23 are both negative power lenses. In a specific embodiment, the first lens 21 and the fourth lens 24 are identical lenses, and the second lens 22 and the third lens 23 are also identical lenses.

[0084] In one embodiment, the first lens 21, the second lens 22, the third lens 23, and the fourth lens 24 all satisfy the following conditions:

[0085] arctan(HI / EFL)≤8 0

[0086] Where HI is the half-image height of the image plane, and EFL is the effective focal length of the imaging lens (20);

[0087] Both the first lens 21 and the fourth lens 24 satisfy the following conditions:

[0088] 0.6≤|F S1 / EFL|≤1

[0089] Among them, F S1 The focal lengths of the first lens 21 and the fourth lens 24;

[0090] Both the second lens 22 and the third lens 23 satisfy the following conditions:

[0091] 1.2≤|F S2 / EFL|≤1.6

[0092] Among them, F S2 The focal lengths of the second lens 22 and the third lens 23 are given.

[0093] In one embodiment, the first lens 21, the second lens 22, the third lens 23, and the fourth lens 24 are all cemented lenses.

[0094] In a preferred embodiment, the imaging lens 20 has an effective focal length (EFL) of 41.69 mm at 565 nm, an object diameter of 7.3 mm, a corrected wavelength range of 500-700 nm, a working F-number of 4.04, a lens-image conjugate distance of 154.7 mm, and arctan(HI / EFL) = 6.32. 0 F S1 =34.47, F S2 = -58.9.

[0095] The specific design parameters of the imaging lens 20 in this embodiment are as follows.

[0096]

[0097]

[0098] The imaging lens 20 provided in this embodiment of the invention has the following advantages: it eliminates various chromatic aberrations, has high imaging quality and low distortion, adopts a symmetrical lens combination, reduces the number of lenses, and lowers the assembly complexity and cost; the lens structure is compact, and sufficient structural space is reserved on the light-incident side and the imaging end, which facilitates lens assembly, adjustment and further mounting of other functional optical components, such as filter components, lens protection components, etc.

[0099] In one embodiment, a filter module 60 is further disposed between the imaging lens 20 and the image analysis module 30, and the filter range of the filter module 60 is adjustable. When the fluorescence excitation module 10 excites the sample 40, the filter module 60 can adjust its own filter range, allowing fluorescence in another wavelength range to pass through the filter module 60 and be imaged on the image sensor in the image analysis module, thereby presenting a fluorescence image on the display module. In a specific embodiment, the filter module 60 is a wheel-type switchable filter module 60, which includes a rotating shaft and a plurality of fluorescence filters 113 on the outer periphery of the rotating shaft, and the filter range of the plurality of fluorescence filters 113 corresponds to the selection of a specific fluorescence wavelength.

[0100] like Figure 6 The figure shows the MTF curve of the lens imaging provided according to the embodiment of the present invention. The MTF curve represents the overall resolution level of an optical system. As can be seen from the figure, the spatial frequency of MTF above 0.5 in the center field of view and the edge field of view can reach 145 line pairs, and the spatial frequency of MTF above 0.3 can reach 225 line pairs, indicating excellent image resolution.

[0101] like Figure 7 The figure shows a lens imaging point diagram provided according to an embodiment of the present invention. As can be seen from the figure, for wavelengths of 515nm, 565nm, 609nm, and 680nm respectively, the root mean square radius of the light spot is <1.2μm, the distribution is relatively uniform, and the light spots are basically within the Airy disk, resulting in a good imaging effect.

[0102] like Figure 8 The image shown is a field curvature / distortion curve detected under visible light according to an embodiment of the present invention. The distortion curve represents the magnitude of distortion under different field of view angles. Figure 7 It can be seen that optical distortion is negative distortion, with a value ≤0.0005%, which is basically distortion-free.

[0103] By using the above-mentioned sample imaging analysis device, the size of the sample imaging analysis device can be reduced, which facilitates the miniaturization of the sample imaging analysis device and reduces the manufacturing and transportation costs of the sample imaging analysis device.

[0104] In the description of this invention, it should be understood that the terms "first" and "second" are used for descriptive purposes only and should not be construed as indicating or implying relative importance or implicitly specifying the number of indicated technical features. Thus, a feature defined as "first" or "second" may explicitly or implicitly include at least one of that feature. In the description of this invention, "a plurality of" means at least two, such as two, three, etc., unless otherwise explicitly specified.

[0105] In this invention, unless otherwise explicitly specified and limited, the terms "installation," "connection," "linking," and "fixing," etc., should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral part; they can refer to a mechanical connection, an electrical connection, or a connection that allows communication between them; they can refer to a direct connection or an indirect connection through an intermediate medium; they can refer to the internal communication of two components or the interaction between two components, unless otherwise explicitly limited. Those skilled in the art can understand the specific meaning of the above terms in this invention according to the specific circumstances.

[0106] In the description of this specification, the references to terms such as "one embodiment," "some embodiments," "example," "specific example," or "some examples," etc., indicate that a specific feature, structure, material, or characteristic described in connection with that embodiment or example is included in at least one embodiment or example of the present invention. In this specification, the illustrative expressions of the above terms do not necessarily refer to the same embodiment or example. Furthermore, the specific features, structures, materials, or characteristics described may be combined in any suitable manner in one or more embodiments or examples. Moreover, without contradiction, those skilled in the art can combine and integrate the different embodiments or examples described in this specification, as well as the features of different embodiments or examples.

[0107] Although embodiments of the present invention have been shown and described above, it is understood that the above embodiments are exemplary and should not be construed as limiting the present invention. Those skilled in the art can make changes, modifications, substitutions and variations to the above embodiments within the scope of the present invention.

Claims

1. A fluorescence excitation module, used in a sample imaging analysis device, characterized in that, The fluorescence excitation module (10) comprises, in sequence along the transmission direction of the optical path: a plurality of excitation units (11) integrated and arranged in one body, each of the plurality of excitation units (11) being configured to emit visible light of different wavelengths; a focusing lens (12) configured to focus the visible light emitted from the plurality of excitation units (11) and transmit the visible light to a transmission optical fiber (13), and the transmission optical fiber (13) having a combination end (131) for receiving the visible light and a plurality of beam splitting ends (132) for illuminating a sample (40).

2. The fluorescence excitation module of claim 1, wherein, The excitation unit (11) comprises, in sequence along the transmission direction of the optical path, a light source (111), a collimating lens (112), a filter (113), and a dichroic mirror (114), the light source (111) emitting visible light of a predetermined wavelength range, the visible light emitted by the light source (111) being able to pass through or reflect on the dichroic mirror (114) until being transmitted to the focusing lens (12).

3. The fluorescence excitation module of claim 1, wherein, Each of the plurality of beam splitting ends (132) is provided with a collimating assembly (50) for collimating the visible light.

4. The fluorescence excitation module of claim 3, wherein, The distance between the beam splitting end (132) and the center point of the sample (40) satisfies the following condition: wherein R is the distance between the beam splitting end (132) and the center point of the sample (40), D is the circular diameter of the sample (40), and NA1 is the numerical aperture of the transmission optical fiber (13).

5. A sample imaging analysis apparatus, characterized by, The fluorescence excitation module (10) according to any one of claims 1 to 4; an imaging lens (20) configured to acquire fluorescence emitted by the sample (40) and obtain a fluorescence image; an image analysis module (30) located on the two sides of the imaging lens (20) respectively, the image analysis module (30) being configured to receive the fluorescence image and analyze and display the fluorescence image. The image analysis module (30) comprises:

6. The sample imaging analysis device of claim 5, wherein, a display unit configured to receive the fluorescence image and display the fluorescence image; an analysis unit configured to analyze the fluorescence image, the analysis unit being located between the display unit and the imaging lens (20). The imaging lens (20) comprises, in sequence along the transmission direction of the optical path, a first lens (21), a second lens (22), a diaphragm, a third lens (23), and a fourth lens (24), the first lens (21) and the fourth lens (24) being mirror-symmetrically arranged, the second lens (22) and the third lens (23) being mirror-symmetrically arranged, the first lens (21) and the fourth lens (24) being positive focal length lenses, and the second lens (22) and the third lens (23) being negative focal length lenses.

7. The sample imaging analysis device of claim 5, wherein, The first lens (21), the second lens (22), the third lens (23), and the fourth lens (24) satisfy the following condition:

8. The sample imaging analysis device of claim 7, wherein, arctan(HI / EFL)≤8° ​ Wherein, HI is half image height of an image plane, and EFL is effective focal length of the imaging lens (20) ; The first lens (21) and the fourth lens (24) both satisfy the following condition: 0.6≤|F S1 / EFL|≤1 wherein F S1 is the focal length of the first lens (21) and the fourth lens (24); The second lens (22) and the third lens (23) both satisfy the following condition: 1.2≤|F S2 / EFL|≤1.6 wherein F S2 is the focal length of the second lens (22) and the third lens (23).

9. The sample imaging analysis device of claim 7, wherein, The first lens (21), the second lens (22), the third lens (23) and the fourth lens (24) are all cemented lenses.

10. The sample imaging analysis device of claim 5, wherein, The imaging lens (20) and the image analysis module (30) are further provided with a filter module (60), and a filter range of the filter module (60) is adjustable.

Images