PCR amplification detection system and detection method
By designing a PCR amplification detection system that adapts to reaction tubes of different shapes, the problem of limited applicability of PCR instrument consumables has been solved, enabling high-throughput detection and experimental convenience, and improving the practicality and economy of PCR instruments.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- HUNAN BIOMETA INTELLIGENT MFG TECH CO LTD
- Filing Date
- 2024-10-31
- Publication Date
- 2026-05-01
AI Technical Summary
Existing PCR instruments can only be used with one type of consumable, which limits their application range and cannot meet the compatibility requirements of consumables of various shapes.
A PCR amplification detection system was designed, comprising a consumable loading module, an optical module, a drive module, and a control module. It has multiple loading chambers and a temperature control component, can adapt to reaction tubes of different shapes, realize fluorescence excitation and acquisition, and precisely regulate the temperature through the temperature control module.
It achieves compatibility and convenience with reaction tubes of different shapes, supports multi-throughput detection, and improves the efficiency and economy of PCR experiments.
Smart Images
Figure CN121950489A_ABST
Abstract
Description
PCR amplification detection system and detection method Technical Field
[0001] This application belongs to the field of PCR detection technology, specifically relating to a PCR amplification detection system and detection method. Background Technology
[0002] Polymerase chain reaction (PCR) is a molecular biology technique used to amplify specific DNA fragments. It can be viewed as a special form of DNA replication outside of a living organism. The most significant characteristic of PCR is its ability to dramatically increase minute amounts of DNA. PCR is a method for the in vitro enzymatic synthesis of specific DNA fragments. It consists of several steps—high-temperature denaturation, low-temperature annealing (renaturation), and optimal-temperature extension—forming a cycle that is repeated cyclically. This allows for rapid amplification of the target DNA, and it is characterized by high specificity, high sensitivity, ease of operation, and time-saving.
[0003] In PCR amplification and detection systems, consumables and reaction reagents are fundamental to PCR experiments. Existing PCR reaction tubes come in various shapes, including round, flat, and multi-tube. However, different shapes of reaction tubes have their own advantages, and different PCR reactions may have different consumable compatibility requirements. However, different PCR instruments can only be matched with consumables of one shape. This limitation of consumable compatibility with a single PCR instrument restricts the scope of PCR instrument use and clearly cannot meet more comprehensive business needs. Summary of the Invention
[0004] The purpose of this application is to provide a PCR amplification detection system and detection method to improve the compatibility of PCR detection equipment with reaction tube consumables of different shapes.
[0005] To achieve the above objectives, this application provides a PCR amplification detection system, which includes:
[0006] The consumable loading module has multiple loading cavities for matching the shape of the consumables;
[0007] The optical module is used for fluorescence excitation and collection of consumables within the loading cavity;
[0008] The driving module is used to drive the optical module to a preset position, so that the optical module moves to the fluorescence excitation and acquisition position; and
[0009] The control module is electrically connected to the drive module and is used to control the start and stop of the drive module according to the working mode of the PCR amplification detection system.
[0010] In the embodiments of this application, the number of loading cavities is two, namely a first loading cavity and a second loading cavity, and the consumable loading module includes:
[0011] The loading body has a first loading cavity and a second loading cavity located at the top and bottom ends of the loading body, respectively.
[0012] A heat-conducting component is disposed on the outer peripheral wall of the loading body, and a temperature sensing element is provided on the heat-conducting component; and
[0013] The temperature control module is electrically connected to the temperature detection element. The temperature control module is used to receive the temperature signal sent by the temperature detection element and adjust the temperature of the consumables according to the temperature signal.
[0014] In embodiments of this application, the temperature control module includes:
[0015] The first temperature control component is located on the side of the heat conduction component away from the outer peripheral wall of the loading body. The first temperature control component is used to regulate the temperature of the first consumable in the first loading cavity or the second consumable in the second loading cavity.
[0016] In embodiments of this application, the temperature control module further includes a second temperature control component disposed on the inner peripheral wall of the second loading cavity, the second temperature control component being used to regulate the temperature of the second consumable in the second loading cavity.
[0017] In the embodiments of this application, the loading body includes two loading parts spliced together. Each loading part includes a first end and a second end connected together. A concave surface is located on the first end. The opposing surfaces of the two first ends have concave surfaces. The two concave surfaces are arranged opposite each other to form a first loading cavity. A second loading cavity is formed between the second ends of the two loading parts.
[0018] In the embodiments of this application, the first end and the second end are connected by a heat-insulating connector in the same loading section.
[0019] In the embodiments of this application, the first end and the second end are integrally formed in the same loading part.
[0020] In embodiments of this application, the optical module includes a fluorescence excitation component and a fluorescence acquisition component. The fluorescence excitation component is used to irradiate the first loading cavity or the second loading cavity and excite fluorescence; the fluorescence acquisition component is used to acquire the fluorescence signal emitted from the first loading cavity or the second loading cavity.
[0021] In the embodiments of this application, the fluorescence excitation component and the fluorescence acquisition component are integrated into a single structure.
[0022] In the embodiments of this application, the fluorescence excitation component and the fluorescence acquisition component are independently arranged and located on both sides of the consumable loading module, and the included angle between the optical axes of the fluorescence excitation component and the fluorescence acquisition component is less than or equal to 90°.
[0023] In embodiments of this application, the fluorescence excitation component includes a light source, a first collimating lens, a first filter, and a first focusing lens that are sequentially connected along the light transmission direction. The first focusing lens is disposed corresponding to a first loading cavity or a second loading cavity. The fluorescence acquisition component includes a second collimating lens, a second filter, a second focusing lens, and a photodetector that are sequentially connected along the light transmission direction. The second collimating lens is disposed corresponding to a first loading cavity or a second loading cavity.
[0024] A second aspect of this application provides a PCR amplification detection method, applied to the PCR amplification detection system described above, the PCR amplification detection method comprising the following steps:
[0025] Select the desired work mode;
[0026] Load the consumables into the corresponding loading chamber according to the required working mode;
[0027] Drive the optical module to move to the preset position;
[0028] The temperature control module is activated to regulate the temperature of the consumables in the loading cavity, so that the consumables are kept within the preset temperature range.
[0029] The optical module is activated to excite and collect fluorescence from the consumables inside the loading cavity.
[0030] In embodiments of this application, the step of activating the temperature control module to regulate the temperature of the consumables in the loading cavity so as to keep the consumables within a preset temperature range further includes:
[0031] Before fluorescence excitation and collection of the second consumable, the temperature of the second loading chamber is acquired in real time and the first temperature control component is activated.
[0032] When the temperature of the second consumable needs to be raised or lowered rapidly, the second temperature control component is activated, and the first and second temperature control components work together.
[0033] Through the above technical solutions, the PCR amplification and detection system provided by the embodiments of the present invention has the following beneficial effects:
[0034] This application enables the consumable loading device to accommodate different types of reaction tubes by forming multiple loading cavities on the consumable loading module to match the shape of the consumables. Before experimental operation, the shape of the reaction tube is determined, and the corresponding reaction tube is installed in the corresponding loading cavity. The optical module is driven by the drive module to move to a preset position corresponding to the corresponding loading cavity, so that the optical module moves to the fluorescence excitation and acquisition position. Then, the optical module is activated to excite and acquire fluorescence from the consumables. This application not only achieves compatibility with various consumable shapes, providing greater compatibility and convenience for experiments, but also allows multiple loading cavities to conduct independent experiments simultaneously through appropriate consumable design, meeting multi-throughput detection requirements. It has strong practicality and economy.
[0035] Other features and advantages of the embodiments of this application will be described in detail in the following detailed description section. Attached Figure Description
[0036] The accompanying drawings are provided to further illustrate the embodiments of this application and form part of the specification. They are used together with the following detailed description to explain the embodiments of this application, but do not constitute a limitation on the embodiments of this application. For those skilled in the art, other drawings can be obtained based on the structures shown in these drawings without any inventive effort. In the drawings:
[0037] Figure 1 is a schematic diagram of the consumable loading module in the PCR amplification detection system according to this application;
[0038] Figure 2 is a schematic diagram of the PCR amplification detection system according to the first embodiment of this application;
[0039] Figure 3 is a schematic diagram of the PCR amplification detection system according to the second embodiment of this application;
[0040] Figure 4 is a schematic diagram of the control flow of the PCR amplification detection method according to this application.
[0041] Explanation of reference numerals in the attached figures
[0042] 11 First loading cavity 63 First filter
[0043] 12 Second loading cavity 64 First focusing lens
[0044] 13 First end 70 Fluorescence acquisition component
[0045] 14 Second end 71 Second collimating lens
[0046] 20 Thermal conductive component 72 Second filter
[0047] 30 First temperature control component 73 Second focusing lens
[0048] 40 Second temperature control component 75 Photodetector
[0049] 50 mounting holes, 76 dichroic mirror
[0050] 60 Fluorescence excitation assembly 77 Lens assembly
[0051] 61 Light source 80 Thermal insulation connector
[0052] 62 First collimating lens 100 consumable loading module Detailed Implementation
[0053] The specific embodiments of this application will be described in detail below with reference to the accompanying drawings. It should be understood that the specific embodiments described herein are for illustration and explanation only and are not intended to limit this application.
[0054] The PCR amplification detection system and detection method according to this application are described below with reference to the accompanying drawings.
[0055] As shown in Figure 2, in an embodiment of this application, a PCR amplification detection system is proposed. The PCR amplification detection system includes a consumable loading module 100, an optical module, a driving module, and a control module. The consumable loading module 100 has multiple loading cavities that match different consumable shapes, as shown in Figure 2. The loading cavity can be two, namely a first loading cavity 11 for matching a first consumable and a second loading cavity 12 for matching a second consumable, so that the consumable loading module 100 can load reaction tubes of different types. The optical module is used to excite and collect fluorescence in the consumables in the first loading cavity 11 or the second loading cavity 12. The driving module is used to drive the optical module to move to a preset position so that the optical module moves to the fluorescence excitation and collection position. The control module is electrically connected to the driving module and is used to control the start and stop of the driving module according to the working mode of the PCR amplification detection system.
[0056] The optical module is slidably mounted on a slide rail. The drive module can use either a linear drive system (drive motor + rotary lead screw) or an electric linear actuator to move the optical module along the linear slide rail to a preset position. It is important to ensure that the movement path of the optical module does not structurally interfere with the consumable loading device. Specifically, when the drive module uses a drive motor + rotary lead screw structure, the optical module is connected to a mounting block on the rotary lead screw. The mounting block and the rotary lead screw are connected by a rotary thread. When the drive motor drives the rotary lead screw to rotate, it can cause the mounting block and the optical module to move linearly together. When the drive module uses an electric linear actuator structure, the drive end of the electric linear actuator is connected to the optical module. When the drive end of the electric linear actuator moves linearly, it can move the optical module.
[0057] In this application, the first loading chamber 11 and the second loading chamber 12 can be switched between. That is, when the instrument is in use, one loading chamber is selected based on the shape of the consumable, while the other loading chamber remains idle. In fact, the two loading chambers can have independent temperature control modes and fluorescence acquisition programs, and the signals within the two loading chambers do not interfere with each other. Therefore, through appropriate consumable design and specific programming, the optical module can be controlled to scan the two loading chambers, allowing them to acquire signals at different time points, thus achieving simultaneous operation of both loading chambers and meeting the detection needs of more than one sample. This application not only achieves compatibility with two different shaped consumables, providing greater compatibility and convenience for experiments, but also allows the two loading chambers to conduct independent experiments simultaneously through appropriate consumable design, meeting multi-throughput detection needs, and possesses strong practicality and economy.
[0058] It should be noted that the first and second consumables of this application may have the same or different shapes. When the first and second consumables have the same shape, both the first and second consumables may be round tubes, flat tubes, or square tubes, etc.; however, the first and second consumables of this application may also have different shapes. For ease of understanding, the following description assumes that the first consumable is a round tube and the second consumable is a flat tube.
[0059] In the embodiments of this application, the consumable loading module 100 includes a loading body, a heat-conducting component 20, and a temperature control module; the first loading cavity 11 and the second loading cavity 12 are located at the top and bottom ends of the loading body, respectively; the heat-conducting component 20 is disposed on the outer peripheral wall of the loading body, and a temperature detection element for detecting the temperature of the loading cavity is provided on the heat-conducting component 20; the temperature control module is electrically connected to the temperature detection element, and the temperature control module is used to receive the temperature signal sent by the temperature detection element and to regulate the temperature of the consumable according to the temperature signal.
[0060] This application determines the shape of the reaction tube before experimental operation, installs the corresponding reaction tube in the first loading chamber 11 or the second loading chamber 12, acquires the temperature of the loading chamber through a temperature detection device, and adjusts the temperature of the loading chamber through a temperature control module, thereby ensuring that the temperature of the consumables in the loading chamber reaches a suitable reaction range and achieves the optimal reaction conditions for the PCR experiment. The heat-conducting component 20 can be a metal block with good thermal conductivity, such as copper or some alloy components; the temperature control module can be a semiconductor cooler (TEC), etc., used to regulate the temperature of the consumables. The temperature detection device is a temperature sensor, used for real-time feedback adjustment of the temperature control module, and achieves precise control of the loading chamber temperature through temperature program settings, providing the temperature conditions required for the PCR experimental reaction.
[0061] In embodiments of this application, the temperature control module includes a first temperature control component 30. The first temperature control component 30 is disposed on the side of the heat-conducting component 20 away from the outer peripheral wall of the loading body. The first temperature control component 30 is used to regulate the temperature of the round tube consumable in the first loading cavity 11 or the flat tube consumable in the second loading cavity 12. The first temperature control component 30 is adapted to the outer peripheral shape of the loading body, completely covering the outer periphery of the loading body, thereby enabling temperature regulation of the round tube consumable in the first loading cavity 11 or the flat tube consumable in the second loading cavity 12.
[0062] In embodiments of this application, the temperature control module further includes a second temperature control component 40 disposed on the inner peripheral wall of the second loading cavity 12. The second temperature control component 40 is used to regulate the temperature of the flat tube consumable within the second loading cavity 12. Further, there are two sets of second temperature control components 40, arranged opposite each other on the inner peripheral wall of the second loading cavity 12. The second temperature control component 40 has a block structure and its area is relatively smaller than that of the first temperature control component 30. During experiments, the second temperature control component 40 can closely adhere to the flat tube consumable, providing faster and more efficient temperature regulation and improving PCR experimental efficiency. Of course, in some cases, if a gentler temperature regulation is required, or for cost considerations, one or both of the second temperature control components 40 can be removed, allowing the flat tube consumable surface to directly contact the heat-conducting component 20, with temperature regulation achieved through the first temperature control component 30 outside the second loading cavity 12.
[0063] In the embodiments of this application, the loading body includes two loading parts spliced together. Each loading part includes a first end 13 and a second end 14 connected together. The concave surface is located on the first end 13. The opposing surfaces of the two first ends 13 have concave surfaces. The two concave surfaces are arranged opposite each other and form a first loading cavity 11. A second loading cavity 12 is formed between the second ends 14 of the two loading parts.
[0064] This solution designs and manufactures a loading structure compatible with different consumable shapes and a corresponding temperature control module. The consumable loading structure consists of two loading parts with concave structures. These two parts are appropriately spliced to form a first loading cavity 11 at the upper position to accommodate round tube consumables, and a second loading cavity 12 at the lower position to accommodate flat consumables. The shapes of the first end 13 and the second end 14 are differentiated to form the first loading cavity 11 adapted to round tube consumables and the second loading cavity 12 adapted to flat tube consumables.
[0065] Furthermore, mounting holes 50 for mounting temperature sensors are provided on the thermally conductive assembly 20 at the corresponding locations of the first end 13 and the second end 14. As shown in Figure 1, there are two temperature sensors, which are respectively installed in the mounting holes 50 on the first end 13 and the second end 14. In addition, more temperature sensors can be set according to actual needs, and their positions can also be adjusted, which is not limited here.
[0066] In the first embodiment of this application, in order to enable the first loading cavity 11 and the second loading cavity 12 to have independent temperature control modes, the first end 13 and the second end 14 are separated, as shown in FIG1. In the same loading part, the first end 13 and the second end 14 are connected by a non-thermally conductive heat-insulating connector 80. In this way, the upper first loading cavity 11 is temperature-controlled by the peripheral first temperature control component 30, and the lower second loading cavity 12 is temperature-controlled by the internal second temperature control component 40 and the peripheral first temperature control component 30, which can achieve faster temperature change and effectively achieve independent temperature control for the upper and lower parts.
[0067] In the second embodiment of this application, the first end portion 13 and the second end portion 14 are integrally formed in the same loading section. In this embodiment, the heat-conducting components 20 and the temperature control modules of the first loading cavity 11 and the second loading cavity 12 are interconnected. In actual working condition, the first loading cavity 11 and the second loading cavity 12 cannot achieve independent temperature control adjustment.
[0068] In embodiments of this application, the optical module includes a fluorescence excitation component 60 and a fluorescence acquisition component 70. The fluorescence excitation component 60 is used to irradiate the first loading cavity 11 or the second loading cavity 12 and excite fluorescence; the fluorescence acquisition component 70 is used to acquire the fluorescence signal emitted from the first loading cavity 11 or the second loading cavity 12.
[0069] The fluorescence excitation component 60 and the fluorescence acquisition component 70 can be multiple modules with different wavelength channels, such as red, yellow, green, and blue. Through folded or stepped optical path multi-channel combination, multiplex PCR detection can be achieved. The connection structure of the fluorescence excitation component 60 and the fluorescence acquisition component 70 can be implemented in two ways: independently and integrated.
[0070] In the first embodiment, the fluorescence excitation component 60 and the fluorescence acquisition component 70 are independently arranged and located on opposite sides of the consumable loading module 100, with the angle between their optical axes less than or equal to 90°. For more efficient fluorescence excitation and acquisition, the central optical axes of the fluorescence excitation component 60 and the fluorescence acquisition component 70 should still be located on the center plane of the gap between the two loading sections. In a preferred embodiment, the reagent liquid level in the loading cavity should be higher than the optical focal point height to ensure efficient fluorescence excitation and acquisition. Furthermore, to reduce the influence of stray excitation light on fluorescence acquisition, the angle between the optical axes of the fluorescence excitation component 60 and the fluorescence acquisition component 70 should be controlled to be no higher than 90°.
[0071] In the embodiments of this application, the fluorescence excitation component 60 includes a light source 61, a first collimating lens 62, a first filter 63, and a first focusing lens 64 that are sequentially connected along the light transmission direction. The first focusing lens 64 is disposed corresponding to the first loading cavity 11 or the second loading cavity 12. The fluorescence acquisition component 70 includes a second collimating lens 71, a second filter 72, a second focusing lens 73, and a photodetector 75 that are sequentially connected along the light transmission direction. The second collimating lens 71 is disposed corresponding to the first loading cavity 11 or the second loading cavity 12.
[0072] The specific optical path of the fluorescence excitation component 60 is as follows: the excitation light emitted by the light source 61 (which can be an LED lamp, laser, etc.) is collimated by the first collimating lens 62, filtered by the first filter 63, and then the light of a specific wavelength is focused by the first focusing lens 64 into the loading cavity of the corresponding consumable. Since the consumable contains a sample and fluorescent substances are added to the sample, the fluorescent substances in the loading cavity can be irradiated, thereby exciting fluorescence. On the other side of the consumable loading module 100 is the fluorescence acquisition component 70. The fluorescence signal generated by the fluorescent substance is collimated by the second collimating lens 71, filtered by the second filter 72, and the fluorescence of a specific wavelength is focused by the second focusing lens 73 onto the photodetector 75 to realize the detection of the fluorescence signal.
[0073] In the second embodiment of this application, as shown in FIG3, the fluorescence excitation component 60 and the fluorescence acquisition component 70 are integrated into one structure. This structure can optimize the structure of the entire PCR amplification detection system, making the entire system smaller in size.
[0074] The fluorescence excitation component 60 includes a light source 61, a first collimating lens 62, and a first filter 63 arranged sequentially along the optical path transmission direction. The fluorescence acquisition component 70 includes a second filter 72, a second focusing lens 73, and a photodetector 75 arranged sequentially along the optical path transmission direction. The lens assembly 77 is used to excite and acquire fluorescence from the sample within the consumable. The fluorescence excited by the fluorescence excitation component 60 is transmitted to the lens assembly 77 via a dichroic mirror 76, and the dichroic mirror 76 can reflect the fluorescence emitted by the lens assembly 77 back to the fluorescence acquisition component 70. In this embodiment, the dichroic mirror is used to transmit the excitation light from the fluorescence excitation component 60 and reflect the fluorescence back to the fluorescence acquisition component 70. In another embodiment, the dichroic mirror can be used to reflect the excitation light from the fluorescence excitation component 60 and transmit the fluorescence back to the fluorescence acquisition component 70.
[0075] As shown in Figure 4, a second aspect of this application provides a PCR amplification detection method, applied to the PCR amplification detection system described above. The PCR amplification detection method includes the following steps:
[0076] Select the desired work mode;
[0077] Load the consumables into the corresponding loading chamber according to the required working mode;
[0078] Drive the optical module to move to the preset position;
[0079] The temperature control module is activated to regulate the temperature of the consumables in the loading cavity, so that the consumables are kept within the preset temperature range.
[0080] The optical module is activated to excite and collect fluorescence from the consumables inside the loading cavity.
[0081] It should be noted that the detection method does not necessarily have to be performed in the above order. For example, it can be performed in the following steps:
[0082] In single-tube operation, consumables are loaded into corresponding loading chambers according to their shape. The position of the optical module is then adjusted to align with the corresponding loading chamber. A PCR cycle experiment is then initiated, repeatedly performing temperature-controlled PCR amplification and fluorescence excitation and acquisition, enabling real-time monitoring of the PCR reaction. For multi-tube scanning mode, the optical module switches between two positions, sequentially performing fluorescence excitation and acquisition, achieving real-time monitoring of the multi-tube PCR reaction. Specifically, based on a preset trigger temperature point, the logic for moving the optical module is that whichever loading chamber completes one round of PCR amplification and reaches the trigger temperature point, the optical module is moved to the corresponding loading chamber position for fluorescence detection. Furthermore, a time-based sequence strategy is used to avoid detection conflicts between the two loading chambers. This application, through the moving scanning method of the optical module, enables independent control and detection of PCR experiments in two loading chambers, improving the versatility of the device. In this application, the preset position of the optical module needs to correspond to the loading cavity and facilitate fluorescence collection and excitation. Since the position of the loading cavity is fixed, at the beginning of the experiment, an optimal position for fluorescence excitation and collection needs to be set, and the optical module is driven to move according to the preset optimal position.
[0083] In embodiments of this application, the step of activating the temperature control module to regulate the temperature of the consumables in the loading cavity so as to keep the consumables within a preset temperature range further includes:
[0084] Before fluorescence excitation and collection of the flat tube consumable, the temperature of the second loading chamber 12 is acquired in real time and the first temperature control component 30 is turned on.
[0085] When the temperature of the flat tube consumable needs to be raised or lowered rapidly, the second temperature control component 40 is activated, and the first temperature control component 30 and the second temperature control component 40 work together.
[0086] Before fluorescence excitation and collection of the flat tube consumables, the temperature of the second loading chamber 12 needs to be monitored in real time to obtain the temperature of the flat tube consumables. When the flat tube consumables are within a suitable temperature range, only the first temperature control component 30 needs to be turned on to regulate the temperature of the flat tube consumables. If the temperature of the flat tube consumables needs to be rapidly increased or decreased during the experiment, the second temperature control component 40, which is in direct contact with the flat tube consumables, also needs to be turned on. The second temperature control component 40 and the first temperature control component 30 work together to meet the temperature regulation of the flat tube consumables, so as to provide faster and more efficient temperature change requirements and improve the efficiency of PCR experiments.
[0087] When performing fluorescence excitation and acquisition on the round tube consumable, it is only necessary to turn on the first temperature control component 30 to monitor the temperature of the round tube consumable.
[0088] In the description of this application, it should be understood that the terms "first" and "second" are used for descriptive purposes only and should not be construed as indicating or implying relative importance or implicitly specifying the number of technical features indicated. Therefore, a feature defined as "first" or "second" may explicitly or implicitly include at least one of that feature. In the description of this application, "multiple" means at least two, such as two, three, etc., unless otherwise explicitly specified.
[0089] In this application, unless otherwise expressly specified and limited, the terms "installation," "connection," "linking," and "fixing," etc., should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral part; they can refer to a mechanical connection, an electrical connection, or a connection that allows communication between components; they can refer to a direct connection or an indirect connection through an intermediate medium; they can refer to the internal communication of two components or the interaction between two components, unless otherwise expressly limited. Those skilled in the art can understand the specific meaning of the above terms in this application based on the specific circumstances.
[0090] In the description of this specification, the references to terms such as "one embodiment," "some embodiments," "example," "specific example," or "some examples," etc., refer to specific features, structures, materials, or characteristics described in connection with that embodiment or example, which are included in at least one embodiment or example of this application. In this specification, the illustrative expressions of the above terms do not necessarily refer to the same embodiment or example. Furthermore, the specific features, structures, materials, or characteristics described may be combined in any suitable manner in one or more embodiments or examples. Moreover, without contradiction, those skilled in the art can combine and integrate the different embodiments or examples described in this specification, as well as the features of different embodiments or examples.
[0091] Although embodiments of this application have been shown and described above, it is understood that the above embodiments are exemplary and should not be construed as limiting this application. Those skilled in the art can make changes, modifications, substitutions and variations to the above embodiments within the scope of this application.
Claims
1. A PCR amplification detection system, characterized in that, The PCR amplification detection system includes: a consumable loading module (100) having multiple loading cavities for matching the shape of the consumables; an optical module for fluorescence excitation and acquisition of the consumables in the loading cavities; a driving module for driving the optical module to move to a preset position so that the optical module moves to the fluorescence excitation and acquisition position; and a control module electrically connected to the driving module, the control module being used to control the start and stop of the driving module according to the working mode of the PCR amplification detection system.
2. The PCR amplification detection system according to claim 1, characterized in that, The number of loading cavities is two, namely a first loading cavity (11) and a second loading cavity (12). The consumable loading module (100) includes: a loading body, wherein the first loading cavity (11) and the second loading cavity (12) are located at the top and bottom ends of the loading body, respectively; a heat-conducting component (20) disposed on the outer peripheral wall of the loading body, wherein a temperature detection element is provided on the heat-conducting component (20); and a temperature control module electrically connected to the temperature detection element, wherein the temperature control module is used to receive the temperature signal sent by the temperature detection element and to regulate the temperature of the consumables according to the temperature signal.
3. The PCR amplification detection system according to claim 2, characterized in that, The temperature control module includes a first temperature control component (30), which is located on the side of the heat conduction component (20) away from the outer peripheral wall of the loading body. The first temperature control component (30) is used to regulate the temperature of the first consumable in the first loading cavity (11) or the second consumable in the second loading cavity (12).
4. The PCR amplification detection system according to claim 2, characterized in that, The temperature control module further includes a second temperature control component (40) disposed on the inner peripheral wall of the second loading cavity (12), the second temperature control component (40) being used to regulate the temperature of the second consumable in the second loading cavity (12).
5. The PCR amplification detection system according to claim 2, characterized in that, The loading body includes two spliced loading parts, each of which includes a first end (13) and a second end (14) connected together. The two first ends (13) have concave surfaces facing each other. The two concave surfaces are arranged opposite each other to form the first loading cavity (11). A second loading cavity (12) is formed between the two second ends (14) of the two loading parts.
6. The PCR amplification detection system according to claim 5, characterized in that, In the same loading section, the first end (13) and the second end (14) are connected by a heat-insulating connector (80).
7. The PCR amplification detection system according to claim 5, characterized in that, The optical module includes a fluorescence excitation component (60) and a fluorescence acquisition component (70). The fluorescence excitation component (60) is used to irradiate the first loading cavity (11) or the second loading cavity (12) and excite fluorescence. The fluorescence acquisition component (70) is used to acquire the fluorescence signal emitted from the first loading cavity (11) or the second loading cavity (12).
8. The PCR amplification detection system according to claim 7, characterized in that, The fluorescence excitation component (60) and the fluorescence acquisition component (70) are integrated into one unit.
9. The PCR amplification detection system according to claim 7, characterized in that, The fluorescence excitation component (60) and the fluorescence acquisition component (70) are independently configured and located on both sides of the consumable loading module (100), and the included angle between the optical axes of the fluorescence excitation component (60) and the fluorescence acquisition component (70) is less than or equal to 90°.
10. The PCR amplification detection system according to claim 9, characterized in that, The fluorescence excitation component (60) includes a light source (61), a first collimating lens (62), a first filter (63), and a first focusing lens (64) connected in sequence along the light transmission direction. The first focusing lens (64) is configured to correspond to the first loading cavity (11) or the second loading cavity (12). The fluorescence acquisition component (70) includes a second collimating lens (71), a second filter (72), a second focusing lens (73), and a photodetector (75) connected in sequence along the light transmission direction. The second collimating lens (71) is configured to correspond to the first loading cavity (11) or the second loading cavity (12).
11. A PCR amplification detection method, characterized in that, In the PCR amplification detection system according to any one of claims 1 to 10, the PCR amplification detection method includes the steps of: selecting the required working mode; loading consumables into the corresponding loading chamber according to the required working mode; and driving the optical module to move to a preset position. The temperature control module is activated to regulate the temperature of the consumables in the loading cavity, so that the consumables are kept within a preset temperature range; the optical module is activated to excite and collect fluorescence from the consumables in the loading cavity.
12. The PCR amplification and detection method according to claim 11, characterized in that, The step of activating the temperature control module to regulate the temperature of the consumables in the loading cavity so as to keep the consumables within a preset temperature range further includes: before performing fluorescence excitation and collection on the second consumables, acquiring the temperature of the second loading cavity (12) in real time and activating the first temperature control component (30); when the temperature of the second consumables needs to be rapidly increased or decreased, activating the second temperature control component (40), with the first temperature control component (30) and the second temperature control component (40) working together.