A wupyv infection model, and a method for constructing the same and use thereof

By constructing a three-dimensional human lung organoid model, the problem of the difficulty in isolating and studying the WUPyV virus was solved, and the infection simulation and characterization of the virus in human lung organs were realized, providing a new research tool for pathogenic mechanisms and immune response mechanisms.

CN122168689APending Publication Date: 2026-06-09STATION OF VIRUS PREVENTION & CONTROL CHINA DISEASES PREVENTION & CONTROL CENT
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
STATION OF VIRUS PREVENTION & CONTROL CHINA DISEASES PREVENTION & CONTROL CENT
Filing Date
2024-12-09
Publication Date
2026-06-09

AI Technical Summary

Technical Problem

Existing technologies are insufficient for effectively isolating and studying WUPyV virus, and the lack of suitable animal models and cell culture methods limits research into its infection mechanism.

Method used

A three-dimensional human lung organoid model was constructed. By culturing proximal differentiated lung organoids and co-infecting them with WUPyV samples, the infection process of the virus in human lung organs was simulated. Specific culture media and matrix gel were used for culturing and isolation.

Benefits of technology

The successful culture and isolation of WUPyV virus provides a new infection model, simulating and characterizing its infection process in human lung organs, and offering a new strategy for studying pathogenic mechanisms and immune response mechanisms.

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Abstract

The application discloses a WUPyV infection model and a construction method and application thereof, and the construction method comprises the following steps: (1) culturing proximal differentiated lung organoids; (2) removing collagen from the lung organoids to prepare lung organoid ball suspension, mixing the lung organoid ball suspension with a WUPyV sample, and co-incubating and infecting; and (3) re-embedding the lung organoids in basement glue after infection, and continuing to culture in a basic culture medium to obtain the WUPyV infection model. The WUPyV infection model is constructed by using three-dimensional human lung organoids, WUPyV can be separated and cultured, the infection process of WUPyV in human lung organs can be better simulated and characterized, and a new infection model is provided for WUPyV infection pathogenesis and body innate immune response mechanism and interaction between viruses and host cells.
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