A filter cartridge pollution prevention device for a Pasteur pipette used in biological sample processing

Abstract

The present application relates to sample supernatant transfer equipment technical field, and disclose a built-in filter element pollution prevention device for biological sample processing, including the platform, the top of the platform is fixedly connected with first connecting frame, the top of first connecting frame is fixedly connected with auxiliary assembly, the top of auxiliary assembly is fixedly connected with second connecting frame, the surface of one end of auxiliary assembly is slidably connected with sliding cover, the surface of the other end of auxiliary assembly is fixedly connected with control center. The both sides of the top of auxiliary assembly are fixedly connected with fixture, the inside of auxiliary assembly is provided with pasteur pipette, the rear end surface of auxiliary assembly is fixedly connected with vertical table, the top of pasteur pipette is provided with funnel shape, the bottom of pasteur pipette is provided with conical shape, the inside of the middle part of pasteur pipette is provided with filter element, the material of filter element is provided with polypropylene, polytetrafluoroethylene and nylon mixed material, the device has the advantages of improving the extraction effect of the device on sample supernatant, convenient for users to use and the like.

Description

Technical Field

[0001] This invention relates to the field of sample supernatant transfer equipment, specifically a pasteurized pipette with built-in filter cartridge for preventing contamination in biological sample processing. Background Technology

[0002] A Pasteur pipette, also known as a dropper, is a commonly used laboratory tool for precisely transferring small amounts of liquid. Its key features are a thin tube with a tapered end and a compressible rubber / plastic bladder at the tip. The liquid is drawn in and released by manually squeezing to create negative or positive pressure.

[0003] Publication No. CN202022800210 discloses a contamination-resistant pipette for cell culture, including a sterilization box. The sterilization box is characterized by: a top cover movably connected to the top of the sterilization box via a rotating shaft; a handle fixedly connected to one side of the top of the top cover; a placement plate and a bottom plate fixedly arranged inside the sterilization box; the placement plate and the bottom plate dividing the space inside the sterilization box from top to bottom into a placement chamber, a sterilization chamber, and a battery chamber; a pipette clamping device movably arranged within the placement chamber and the sterilization chamber; a plurality of placement slots fixedly opened on the placement plate; the pipette clamping device movably arranged within the placement slots; an elastic rubber bladder at the top of the pipette clamping device; a fixed opening fixedly connected to the top of the elastic rubber bladder; a rubber plug at the top of the fixed opening; and a first elastic metal clip and a second elastic metal clip symmetrically arranged at the bottom of the elastic rubber bladder; a conduit disposed inside the first elastic metal clip. This device can continuously sterilize the pipette when it is idle, preventing bacterial contamination before reuse. In existing biological liquid sample testing in practical research, Pasteur pipettes are typically used to aspirate and transfer the supernatant from the sample. During this process, the user needs to constantly squeeze the Pasteur pipette to create negative pressure at the bottom, thus successfully drawing the sample supernatant into the pipette. However, in actual testing, some sample supernatants contain suspended solids and other impurities. When using a Pasteur pipette to extract the sample supernatant, some of these suspended solids and impurities are also extracted into the pipette. In subsequent testing, these suspended solids and impurities are likely to affect the experimental results, resulting in poor actual performance of the device in extracting and transporting sample supernatant. There is room for further improvement in the extraction efficiency of existing devices for sample supernatant. Summary of the Invention

[0004] To address the shortcomings of existing technologies, this invention provides a built-in filter cartridge anti-contamination device for pasteurized pipettes used in biological sample processing, which has advantages such as improving the extraction efficiency of sample supernatant and being easy for users to use.

[0005] To achieve the above objectives, the present invention provides the following technical solution: a pasteurized pipette with built-in filter cartridge anti-contamination device for biological sample processing, comprising: a base, a first connecting frame, a second connecting frame, a first linear drive assembly, an electromagnet, a locking block, an active chamber, a slot, a permeation tank, a sealing ring, a magnetic suction plate, a stop block, a shielding component, a side plate, a first elastic component, a guardrail, a clamp, a stand, an auxiliary assembly, a control center, a sliding cover, a second linear drive assembly, a top plate, a push plate, a fixing plate, a through rod, a locking plate, a fourth linear drive assembly, an output head, a push rod, a diversion chamber, a baffle, a top plate, an adjustment chamber, a second elastic component, a delivery pipe, a pasteurized pipe, a pressure layer, a filter cartridge, a sealing plate, a connecting plate, and a fixing ring.

[0006] The positions and connections of the above structures are as follows: The Pasteur pipette with built-in filter for biological sample processing includes a base. A first connecting frame is fixedly connected to the top of the base. An auxiliary component is fixedly connected to the top of the first connecting frame. A second connecting frame is fixedly connected to the top of the auxiliary component. A sliding cover is slidably connected to one end of the auxiliary component, and a control center is fixedly connected to the other end. Clamps are fixedly connected to both sides of the top of the auxiliary component. A Pasteur tube is installed inside the auxiliary component, and a stand is fixedly connected to the rear end of the auxiliary component.

[0007] Preferably, the top of the Pasteur tube is funnel-shaped, the bottom of the Pasteur tube is cone-shaped, a filter element is disposed inside the middle part of the Pasteur tube, the filter element is made of a mixture of polypropylene, polytetrafluoroethylene and nylon, and a fixing ring is fixedly connected to the top of the filter element, the fixing ring being fixedly connected inside the Pasteur tube.

[0008] Preferably, a first linear drive assembly is fixedly connected to the bottom of the first connecting frame. The first linear drive assembly is specifically an electric push rod. An electromagnet is fixedly connected to the bottom output end of the first linear drive assembly. Both ends of the electromagnet are fixedly connected to a locking block, and the bottom edge of the locking block is rounded. An active chamber is provided at the bottom of the first linear drive assembly. A sealing ring is fixedly connected to the outer surface of the active chamber. A slot is opened at the top center of the active chamber. The electromagnet and the two locking blocks extend into the interior of the active chamber through the slot. A magnetic suction plate is fixedly connected to the center of the bottom inner wall of the active chamber.

[0009] Preferably, a third linear drive assembly is fixedly connected to both sides of the auxiliary component. The third linear drive assembly is specifically an electric push rod. A push plate is fixedly connected to the output end of the third linear drive assembly near its symmetrical plane. A fixing plate is fixedly connected to the end of the push plate near its symmetrical plane. A through rod is fixedly connected to the bottom of the fixing plate. A clamping plate is slidably connected inside the through rod and the clamping plate is limited at the through rod. A sealing plate is fixedly connected to both sides of the bottom of the filter element. The sealing plate is arc-shaped and a connecting plate is fixedly connected to the end of the sealing plate away from the filter element. The shape and size of the connecting plate are adapted to the clamping plate. The two connecting plates pass through the Barcol tube and extend to the outer sides of the left and right ends of the Barcol tube. The extended part of the connecting plate is clamped inside the clamping plate.

[0010] Preferably, a fourth linear drive assembly is fixedly connected to the top of the fixed plate. An output head is fixedly connected to the top output end of the fourth linear drive assembly. Two push rods are fixedly connected to the top of the output head. A diversion chamber is provided on the top of the output head. Adjustment chambers are fixedly connected to both the front and rear ends of the diversion chamber. A baffle is sleeved inside the diversion chamber. The baffle extends through and to the outer top of the diversion chamber, and a cover plate is fixedly connected to the extension of the baffle. A push rod is fixedly connected to the end of the output head near the adjustment chamber. The push rod extends through the adjustment chamber and into the interior of the adjustment chamber. The extension of the push rod is fixedly connected to the baffle. A second elastic element, specifically a spring, is fixedly connected to the top of the adjustment chamber. The other end of the second elastic element is fixedly connected to the top of the extension of the push rod. The two diversion chambers extend through and into the interior of the Bartholin's tube and are located on the top of the filter element. A conveying pipe is fixedly connected to the other end of the diversion chamber. The other end of the conveying pipe extends to the outer rear end of the auxiliary assembly.

[0011] Preferably, a pressure-resistant layer is provided in the upper middle section of the Bartholin tube. The pressure-resistant layer is made of soft rubber and is located at the bottom of the diversion chamber. A second linear drive assembly is fixedly connected to the rear end of the sliding cover and the auxiliary components. The second linear drive assembly is specifically an electric push rod. A top plate is fixedly connected to the output end of the second linear drive assembly. The size of the top plate is adapted to the size of the pressure-resistant layer.

[0012] Preferably, the activity room has permeation grooves on both sides of the top, extending to the bottom of the activity room. A guardrail is fixedly connected to one end of the activity room near the permeation groove. A stop block is slidably connected to both sides of the magnetic plate. A blocking member is fixedly connected to the end of the stop block away from the magnetic plate, and the shape and size of the blocking member are adapted to the permeation groove. The end of the blocking member near the magnetic plate has rounded corners. Side plates are fixedly connected to the front and rear ends of the stop block. A first elastic member, specifically a spring, is fixedly connected to the end of the side plate away from the magnetic plate. The other end of the first elastic member is fixedly connected to the inner wall of the activity room.

[0013] Preferably, the control center includes a drive and force control module, a process and logic module, a user interaction module, and an advanced function integration module, which are electrically connected to each other.

[0014] Beneficial effects 1. This pasteurized pipette for biological sample processing has a built-in filter cartridge to prevent contamination. By activating the auxiliary components, the device can filter the sample supernatant while extracting samples, improving the extraction quality and sampling efficiency of the device and making it easier for users to use.

[0015] 2. This pasteurized pipette for biological sample processing has a built-in filter cartridge anti-contamination device. By activating the auxiliary components, the device reduces the likelihood of some sample supernatant remaining in the filter cartridge, making it difficult to recover. This sample supernatant, along with the filter cartridge and pasteurized pipette, is then discarded, improving the recovery efficiency of the sample supernatant and making it easier for users to use.

[0016] 3. The pasteurized pipette used for biological sample processing has a built-in filter cartridge anti-contamination device. By activating the auxiliary components, the device reduces the possibility of sample supernatant flowing back to the filter cartridge and re-introducing suspended matter, thereby improving the transfer efficiency of the device and making it easier for users to use. Attached Figure Description

[0017] Figure 1 This is a schematic diagram of the external structure of the Pasteur pipette built-in filter anti-contamination device for biological sample processing according to the present invention; Figure 2 This is a schematic diagram of the sliding cover movement structure of the anti-contamination device with built-in filter element in the Pasteur pipette used for biological sample processing according to the present invention; Figure 3 This is a rear view schematic diagram of the anti-contamination device with built-in filter element in the Pasteur pipette used for biological sample processing according to the present invention. Figure 4 This is a schematic diagram of the internal structure of the sliding cover of the anti-contamination device with built-in filter element for the Pasteur pipette used in biological sample processing according to the present invention; Figure 5 This is a schematic diagram of the internal structure of the anti-contamination device with built-in filter in the Pasteur pipette used for biological sample processing according to the present invention. Figure 6 This is a schematic diagram of the push plate structure of the Pasteur pipette built-in filter anti-contamination device for biological sample processing according to the present invention; Figure 7 This is a schematic diagram of the external structure of the Pasteur pipette with built-in filter for preventing contamination in biological sample processing according to the present invention. Figure 8 This is a schematic diagram of the filter element structure of the Pasteur pipette built-in filter element anti-contamination device for biological sample processing according to the present invention; Figure 9This is a schematic diagram of the diversion chamber structure of the Pasteur pipette built-in filter cartridge anti-contamination device for biological sample processing according to the present invention; Figure 10 This is a schematic diagram of the internal structure of the adjustment chamber of the Pasteur pipette built-in filter anti-contamination device for biological sample processing according to the present invention; Figure 11 This is a schematic diagram of the anti-contamination device block structure of the built-in filter cartridge of the Pasteur pipette used for biological sample processing according to the present invention; Figure 12 This is a schematic diagram of the internal structure of the active chamber of the Pasteur pipette built-in filter anti-contamination device for biological sample processing according to the present invention.

[0018] In the diagram: 1. Platform; 10. First connecting frame; 11. Second connecting frame; 12. First linear drive assembly; 120. Electromagnet; 121. Locking block; 13. Activity chamber; 130. Slot; 131. Infiltration tank; 132. Sealing ring; 133. Magnetic suction plate; 134. Abutment; 135. Blocking component; 136. Side plate; 137. First elastic component; 138. Guardrail; 14. Clamp; 15. Stand; 3. Auxiliary assembly; 30. Control center; 31. Sliding cover; 310. 311. Second linear drive assembly; 32. Top plate; 32. Push plate; 320. Fixing plate; 321. Through rod; 322. Clamping plate; 323. Fourth linear drive assembly; 324. Output head; 3240. Push rod; 325. Diverting chamber; 3250. Baffle; 3251. Cover plate; 326. Adjusting chamber; 3260. Second elastic element; 327. Conveying pipe; 4. Barcol tube; 40. Pressure layer; 41. Filter element; 410. Sealing plate; 411. Connecting plate; 42. Fixing ring. Detailed Implementation

[0019] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0020] Example Please see Figures 1 to 3A pasteurized pipette with built-in filter for preventing contamination during biological sample processing includes a base 1, a first connecting frame 10 fixedly connected to the top of the base 1, an auxiliary component 3 fixedly connected to the top of the first connecting frame 10, a second connecting frame 11 fixedly connected to the top of the auxiliary component 3, a sliding cover 31 slidably connected to one end of the auxiliary component 3, and a control center 30 fixedly connected to the other end of the auxiliary component 3. Clamps 14 are fixedly connected to both sides of the top of the auxiliary component 3, a pasteurized pipette 4 is disposed inside the auxiliary component 3, and a stand 15 is fixedly connected to the rear end of the auxiliary component 3. In existing biological liquid sample testing in actual research, Pasteur pipettes are usually used to aspirate and transfer the supernatant from the sample. During this process, the user needs to squeeze the Pasteur pipette constantly to create negative pressure at the bottom of the pipette, so as to successfully draw the sample supernatant into the pipette. However, in actual testing, some sample supernatants contain suspended matter and other impurities. When using Pasteur pipettes to extract the sample supernatant, some of the suspended matter and impurities are also extracted into the Pasteur pipette. In subsequent testing, these suspended matter and impurities are likely to affect the experimental results, resulting in poor actual performance of the device in extracting and transporting sample supernatant. This invention discloses a contamination prevention device for a Pasteur pipette with an internal filter cartridge 41 for biological sample processing. The sample is placed on top of a platform 1. Initially, the two diversion channels are separated from the Pasteur tube 4, the active chamber 13 is not located inside the top side of the Pasteur tube 4, the two clamping plates 322 do not contact the two connecting plates 411, and the electromagnet 120 and the magnetic suction plate 133 attract each other. When the user needs to extract and transfer the sample supernatant, the user can manually pull open the sliding cover 31, place the top part of the Pasteur tube 4 into the two clamps 14, and use the control center 30 to activate the two second linear drive components 310. The activation of the two second linear drive components 310 pushes out and moves the push plate 32. The movement of the push plate 32 drives the clamping plates 322 via the fixing plate 320. The diversion chamber 325 moves until the connecting plate 411 is engaged inside the clamping plate 322 and the diversion chamber 325 extends into the inside of the Parshall tube 4. The sliding cover 31 is then reset. At this time, the two top plates 311 at the front and rear ends are directly opposite the pressure layer 40 of the Parshall tube 4. At this time, the user can manually control the control center 30 to open the first linear drive assembly 12. The opening of the first linear drive assembly 12 drives the movable chamber 13 and the sealing ring 132 to move through the electromagnet 120 and the magnetic suction plate 133 until the movable chamber 13 and the sealing ring 132 move into the funnel-shaped inside of the Parshall tube 4. This application uses the auxiliary assembly 3 and the Parshall tube 4 to filter out the suspended matter in the extracted sample supernatant, thereby improving the extraction quality of the sample supernatant and making it easier for the user to use. Please see Figures 5 to 7Furthermore, in the above description, the top of the Pasteur tube 4 is set in a funnel shape, the bottom of the Pasteur tube 4 is set in a cone shape, and a filter element 41 is provided inside the middle part of the Pasteur tube 4. The filter element 41 is made of a mixture of polypropylene, polytetrafluoroethylene and nylon. A fixing ring 42 is fixedly connected to the top of the filter element 41 and is fixedly connected inside the Pasteur tube 4. After the Pasteur tube 4 is installed in the above steps, its conical bottom part is submerged in the sample. At this time, the user can draw the supernatant from the sample into the Pasteur tube 4 by generating negative pressure inside the Pasteur tube 4. During the extraction of the sample supernatant, it first passes through the filter element 41. The filter element 41 filters the suspended matter inside the sample supernatant and then transports the sample supernatant to the top side inside the Pasteur tube 4. This completes the filtration of the sample supernatant while extracting, improving the extraction quality and sampling efficiency of the device and making it easier for users to use. Please see Figures 5 to 11 Furthermore, as described above, a first linear drive assembly 12 is fixedly connected to the bottom of the first connecting frame 10. The first linear drive assembly 12 is specifically an electric push rod. An electromagnet 120 is fixedly connected to the bottom output end of the first linear drive assembly 12. Both ends of the electromagnet 120 are fixedly connected to a locking block 121, and the bottom edge of the locking block 121 is rounded. An active chamber 13 is provided at the bottom of the first linear drive assembly 12. A sealing ring 132 is fixedly connected to the outer surface of the active chamber 13. A slot 130 is provided at the top center of the active chamber 13. The electromagnet 120 and the two locking blocks 121 extend into the interior of the active chamber 13 through the slot 130. A magnetic suction plate 133 is fixedly connected to the center of the bottom inner wall of the active chamber 13. Because the presence of filter element 41 filters suspended matter, the sample supernatant must pass through filter element 41 during transport, resulting in a significantly slower flow rate than a regular pipette, especially when processing viscous samples. In the above steps, the control center 30 activates the first linear drive assembly 12, which moves the electromagnet 120 upward. The movement of the electromagnet 120, via the magnetic suction plate 133, moves the movable chamber 13 and the sealing ring 132 upward inside the top side of the Parshall tube 4. At this time, a negative pressure is formed inside the Parshall tube 4, drawing out the sample supernatant. This application utilizes the first linear drive assembly 12, the movable chamber 13, the sealing ring 132, and the Parshall tube. 4. The funnel-shaped structure at the top forms a piston-like structure, which replaces manual squeezing by increasing the negative pressure delivery force per unit time. The movement of the movable chamber 13 and the sealing ring 132 provides uniform and controllable negative pressure. The magnitude and speed of the negative pressure can be precisely controlled by the upward movement distance of the movable chamber 13 of the first linear drive assembly 12, which significantly improves the flow rate and consistency, provides constant and programmable suction inside the Parshall tube 4, avoids flow rate fluctuations caused by uneven manual operation, and the Parshall tube 4 itself is still used as a disposable item, reducing the impact of the filter element 41 on the extraction efficiency of the sample supernatant and further improving the extraction efficiency of the device, making it convenient for users to use. Please see Figures 5 to 10 Furthermore, in the above description, a third linear drive assembly is fixedly connected to both sides of the auxiliary component 3. The third linear drive assembly is specifically an electric push rod. A push plate 32 is fixedly connected to the output end of the third linear drive assembly near its symmetrical plane. A fixing plate 320 is fixedly connected to the end of the push plate 32 near its symmetrical plane. A through rod 321 is fixedly connected to the bottom of the fixing plate 320. A clamping plate 322 is slidably connected inside the through rod 321, and the clamping plate 322 is limited at the through rod 321. A sealing plate 410 is fixedly connected to both sides of the bottom of the filter element 41. The sealing plate 410 is arc-shaped, and a connecting plate 411 is fixedly connected to the end of the sealing plate 410 away from the filter element 41. The shape and size of the connecting plate 411 are adapted to the clamping plate 322. The two connecting plates 411 pass through the Barcol tube 4 and extend to the outer sides of the left and right ends of the Barcol tube 4. The extended part of the connecting plate 411 is clamped inside the clamping plate 322. After the sample supernatant extraction is completed, the remaining sample supernatant inside the filter element 41 is difficult to recover due to its material properties, resulting in poor extraction efficiency. In the above steps, the connecting plate 411 is engaged inside the clamping plate 322, and the sealing plate 410 blocks the connection between the connecting plate 411 and the Parshall tube 4. After the Parshall tube 4 has extracted the sample supernatant from the sample, the user can manually push the two clamping plates 322. Guided by the through rod 321, the clamping plates 322 move upward under the influence of the thrust. The movement of the clamping plates 322 causes the connecting plate 411 and the sealing plate 410 to move. The movement of the sealing plate 410 causes the bottom part of the filter element 41 to move towards the top part of the filter element 41, thereby squeezing the inside of the filter element 41. Since the suspended matter is transported from the bottom to the top of the filter element 41, this part of the suspended matter forms a whole after being squeezed. When the filter element 41 is squeezed, the sample supernatant inside is blocked by the squeezed suspended matter. Most of the sample supernatant can continue to flow into the top of the filter element 41 and be adsorbed and extracted by the movement of the moving chamber 13 and the sealing ring 132. Only a part of the sample supernatant penetrates to the bottom of the filter element 41 and falls back to the sample. This part of the fallen sample supernatant can be re-adsorbed after the second Parshall tube 4 is installed. Thus, the device reduces the situation where some sample supernatant is left in the filter element 41 and is difficult to recover, and this part of the sample supernatant is discarded along with the filter element 41 and the Parshall tube 4. It improves the recovery effect of the sample supernatant and is convenient for users to use. Please see Figures 5 to 10 Further, as described above, a fourth linear drive assembly 323 is fixedly connected to the top of the fixed plate 320. An output head 324 is fixedly connected to the top output end of the fourth linear drive assembly 323. Two push rods 3240 are fixedly connected to the top of the output head 324. A diversion chamber 325 is provided on the top of the output head 324. Adjustment chambers 326 are fixedly connected to both the front and rear ends of the diversion chamber 325. A baffle 3250 is sleeved inside the diversion chamber 325. The baffle 3250 penetrates and extends to the outer top of the diversion chamber 325, and a cover plate 3251 is fixedly connected to the extension of the baffle 3250. The end of the output head 324 near the adjustment chamber 326 is... A top rod 3240 is fixedly connected, which passes through the adjustment chamber 326 and extends into the interior of the adjustment chamber 326. The extended portion of the top rod 3240 is fixedly connected to the baffle 3250. A second elastic element 3260, which is specifically a spring, is fixedly connected to the top of the extended portion of the top rod 3240. Two diversion chambers 325 pass through and extend into the interior of the Bartholin tube 4 and are located on the top of the filter element 41. A conveying pipe 327 is fixedly connected to the other end of the diversion chamber 325. The other end of the conveying pipe 327 extends to the outer rear end of the auxiliary component 3. After the sample supernatant is extracted, it needs to be transferred to another container. During the transfer, if the sample supernatant passes through the filter element 41, suspended matter may be reintroduced into the sample supernatant. At this time, the user can activate the two fourth linear drive components 323 through the control center 30. The activation of the fourth linear drive components 323 drives the output head 324 to move upward. The movement of the output head 324 drives the two push rods 3240 to move upward. Initially, the baffle 3250 blocks the inside of the diversion chamber 325, preventing the sample supernatant from being discharged through the diversion chamber 325 during extraction. When the push rods 3240 move upward, they drive the baffle 3250 to move upward, causing the second elastic element 3260 to compress and deform. When the baffle 3250 moves to no longer obstruct the flow chamber 325, the user can use the control center 30 to activate the first linear drive assembly 12 to drive the movable chamber 13 and the sealing ring 132 downward. At this time, the internal top side air pressure of the Bartholin tube 4 increases, and the extracted sample supernatant is transported to the two flow chambers 325. The two flow chambers 325 then transport the sample supernatant to the two delivery pipes 327. During this period, the user needs to place the container on the stand 15. The delivery pipe 327 then transports the sample supernatant to the container on the top of the stand 15. In this way, the device completes the transfer of the sample supernatant. During the transfer process, the sample supernatant is reduced from flowing back to the filter element 41 and bringing in suspended matter again, which improves the transfer effect of the device and makes it easier for the user to use. Please see Figures 3 to 7 Furthermore, in the above description, a pressure layer 40 is provided in the middle and upper section of the Bartholin tube 4. The pressure layer 40 is made of soft rubber and is located at the bottom of the diversion chamber 325. A second linear drive assembly 310 is fixedly connected to the rear end of the sliding cover 31 and the auxiliary assembly 3. The second linear drive assembly 310 is specifically an electric push rod. A top plate 311 is fixedly connected to the output end of the second linear drive assembly 310. The size of the top plate 311 is adapted to the size of the pressure layer 40. During the above steps, while the fourth linear drive component 323 moves, the internal program of the control center 30 activates two second linear drive components 310. The activation of the second linear drive components 310 drives the two cover plates 3251 to move. The two cover plates 3251 squeeze the pressure layer 40 of the Barcol tube 4 one in front and one behind, so that the sample supernatant cannot fall through the pressure layer 40 of the Barcol tube 4 to the filter element 41. This works in conjunction with the above-mentioned diversion chamber 325 to prevent the sample supernatant from flowing back to the filter element 41 and causing it to be re-contaminated with suspended matter, making it convenient for users to use. Please see Figures 11 to 12Furthermore, as described above, the activity room 13 has permeation grooves 131 on both sides of the top and extends to the bottom of the activity room 13. Inside the activity room 13, a guardrail 138 is fixedly connected to one end near the permeation groove 131. Abutments 134 are slidably connected to both sides of the magnetic suction plate 133. A shielding member 135 is fixedly connected to one end of the abutment 134 away from the magnetic suction plate 133, and the shape and size of the shielding member 135 are adapted to the permeation groove 131. The shielding member 135 is rounded at one end near the magnetic suction plate 133. Side plates 136 are fixedly connected to the front and rear ends of the abutment 134. A first elastic member 137 is fixedly connected to one end of the side plate 136 away from the magnetic suction plate 133. The first elastic member 137 is specifically a spring. The other end of the first elastic member 137 is fixedly connected to the inner wall of the activity room 13. In the initial stage of Parshall tube 4 extraction, before the sample is placed on platform 1, the pores of the drying filter element 41 are filled with air. The sample supernatant needs to overcome surface tension to "squeeze out" the air before it can pass through, resulting in extremely high resistance. This makes it difficult for the Parshall tube 4 to extract the sample, and the flow rate is extremely slow and unstable. Initially, when the electromagnet 120 and the magnetic plate 133 attract each other, the two locking blocks 121 apply a pushing force away from the magnetic plate 133 to the two abutment blocks 134 respectively, the two first elastic elements 137 undergo compressive elastic deformation, and the two blocking elements 135 block the permeation tank 131. At this time, the internal shape of the active chamber 13 is as follows: The space is enclosed. During this period, the user can use the control center 30 to turn off the electromagnet 120 and turn on the first linear drive component 12 to move the electromagnet 120 upward in the active chamber 13, but without leaving the active chamber 13. At this time, the electromagnet 120 and the magnetic suction plate 133 no longer attract each other. After the electromagnet 120 moves, the locking block 121 no longer provides pushing force to the two abutments 134. The first elastic element 137 elastically deforms and resets, generating elastic force that acts on the abutments 134, causing the abutments 134 to move. The movement of the abutments 134 causes the blocking element 135 to move on the surface of the guardrail 138. At this time, the blocking element 135 moves. When component 135 no longer blocks the permeation tank 131, the user can pour a portion of a buffer solution, such as physiological saline, that will not affect the sample supernatant into the top side of the Pasteur tube 4. At this time, the physiological saline enters the Pasteur tube 4 through the permeation tank 131 and falls into the filter element 41, wetting the filter element 41. Since the electromagnet 120 itself has not detached from the active chamber 13, the structure at the top of the electromagnet 120 blocks the connection between the electromagnet 120 and the active chamber 13. When all the physiological saline has entered the Pasteur tube 4, the control center 30 reopens the electromagnet 120. 134. The shielding component 135 resets, causing the interior of the active chamber 13 to form a closed space again. The first linear drive component 12 drives the active chamber 13 to move downward, increasing the air pressure inside the Bartholin tube 4. This causes the saline solution at the top of the filter element 41 to be transported through the filter element 41 to the bottom of the Bartholin tube 4 for discharge. When the saline solution soaks the filter element 41 and replaces the air inside, the air pressure can force out a portion of the saline solution inside the filter element 41, minimizing dead space residue, reducing the dilution of subsequent sample supernatant, improving the extraction effect of the sample supernatant, and making it easier for users to use. Please see Figures 1 to 3 Furthermore, as described above, the control center 30 includes a drive and force control module, a process and logic module, a user interaction module, and an advanced function integration module, which are electrically connected to each other. The control center 30 is used to control the start and stop of a series of components such as the auxiliary component 3, the first linear drive component 12 and the electromagnet 120, to ensure the normal operation of the device.

[0021] Although embodiments of the invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A pasteurized pipette with built-in filter cartridge anti-contamination device for biological sample processing, comprising a base (1), characterized in that: The top of the platform (1) is fixedly connected to a first connecting frame (10), the top of the first connecting frame (10) is fixedly connected to an auxiliary component (3), the top of the auxiliary component (3) is fixedly connected to a second connecting frame (11), a sliding cover (31) is slidably connected to one end surface of the auxiliary component (3), and a control center (30) is fixedly connected to the other end surface of the auxiliary component (3). Clamps (14) are fixedly connected to both sides of the top of the auxiliary component (3), a Bartholin's tube (4) is installed inside the auxiliary component (3), and a platform (15) is fixedly connected to the rear end surface of the auxiliary component (3).

2. The anti-contamination device with built-in filter cartridge for pasteurized pipettes used for biological sample processing according to claim 1, characterized in that: The top of the Bartholomew's tube (4) is funnel-shaped, and the bottom of the Bartholomew's tube (4) is cone-shaped. A filter element (41) is provided inside the middle part of the Bartholomew's tube (4). The filter element (41) is made of a mixture of polypropylene, polytetrafluoroethylene and nylon. A fixing ring (42) is fixedly connected to the top of the filter element (41). The fixing ring (42) is fixedly connected inside the Bartholomew's tube (4).

3. The anti-contamination device with built-in filter cartridge for pasteurized pipettes used for biological sample processing according to claim 1, characterized in that: The first linear drive assembly (12) is fixedly connected to the bottom of the first connecting frame (10). The first linear drive assembly (12) is specifically an electric push rod. An electromagnet (120) is fixedly connected to the bottom output end of the first linear drive assembly (12). Both ends of the electromagnet (120) are fixedly connected to a locking block (121), and the bottom edge of the locking block (121) is rounded. An active chamber (13) is provided at the bottom of the first linear drive assembly (12). A sealing ring (132) is fixedly connected to the outer surface of the active chamber (13). A slot (130) is opened at the top center of the active chamber (13). The electromagnet (120) and the two locking blocks (121) extend into the interior of the active chamber (13) through the slot (130). A magnetic suction plate (133) is fixedly connected to the center of the bottom inner wall of the active chamber (13).

4. The anti-contamination device with built-in filter cartridge for pasteurized pipettes used for biological sample processing according to claim 1, characterized in that: The auxiliary component (3) has a third linear drive component fixedly connected to both sides inside. The third linear drive component is specifically an electric push rod. A push plate (32) is fixedly connected to the output end of the third linear drive component near its symmetrical plane. A fixed plate (320) is fixedly connected to the end of the push plate (32) near its symmetrical plane. A through rod (321) is fixedly connected to the bottom of the fixed plate (320). A locking plate (322) is slidably connected inside the through rod (321), and the locking plate (322) is positioned at the through rod (321). For the limiting treatment, a sealing plate (410) is fixedly connected to both sides of the bottom of the filter element (41). The sealing plate (410) is set in an arc shape and a connecting plate (411) is fixedly connected to the end of the sealing plate (410) away from the filter element (41). The shape and size of the connecting plate (411) are adapted to the clamping plate (322). The two connecting plates (411) pass through the barium tube (4) and extend to the outer side of the left and right ends of the barium tube (4). The extended part of the connecting plate (411) is clamped inside the clamping plate (322).

5. The anti-contamination device with built-in filter cartridge for pasteurized pipettes used for biological sample processing according to claim 3, characterized in that: A fourth linear drive assembly (323) is fixedly connected to the top of the fixed plate (320). An output head (324) is fixedly connected to the top output end of the fourth linear drive assembly (323). Two push rods (3240) are fixedly connected to the top of the output head (324). A diversion chamber (325) is provided on the top of the output head (324). Adjustment chambers (326) are fixedly connected to both the front and rear ends of the diversion chamber (325). A baffle (3250) is sleeved inside the diversion chamber (325). The baffle (3250) penetrates and extends to the outer side of the top of the diversion chamber (325), and a cover plate (3251) is fixedly connected to the extension of the baffle (3250). A top rod is fixedly connected to the end of the output head (324) near the adjustment chamber (326). A rod (3240) and a top rod (3240) pass through the regulating chamber (326) and extend into the interior of the regulating chamber (326). The extension of the top rod (3240) is fixedly connected to a baffle (3250). A second elastic element (3260) is fixedly connected to the top of the regulating chamber (326). The second elastic element (3260) is specifically a spring. The other end of the second elastic element (3260) is fixedly connected to the top of the extension of the top rod (3240). Two diversion chambers (325) pass through and extend into the interior of the Bartholin's tube (4). The diversion chambers (325) are located on the top of the filter element (41). The other end of the diversion chambers (325) is fixedly connected to a conveying pipe (327). The other end of the conveying pipe (327) extends to the outer rear end of the auxiliary component (3).

6. The anti-contamination device with built-in filter cartridge for pasteurized pipettes used for biological sample processing according to claim 5, characterized in that: A pressure-resistant layer (40) is provided in the middle and upper section of the Bartholin tube (4). The pressure-resistant layer (40) is made of soft rubber. The pressure-resistant layer (40) is located at the bottom of the diversion chamber (325). A second linear drive assembly (310) is fixedly connected to the rear end of the sliding cover (31) and the auxiliary assembly (3). The second linear drive assembly (310) is specifically an electric push rod. A top plate (311) is fixedly connected to the output end of the second linear drive assembly (310). The size of the top plate (311) is adapted to the size of the pressure-resistant layer (40).

7. The anti-contamination device with built-in filter cartridge for pasteurized pipettes used for biological sample processing according to claim 3, characterized in that: The activity room (13) has permeation grooves (131) on both sides of the top, and the permeation grooves (131) extend to the bottom of the activity room (13). Inside the activity room (13), a guardrail (138) is fixedly connected to one end of the permeation groove (131). Abutments (134) are slidably connected to both sides of the magnetic suction plate (133). A blocking member (135) is fixedly connected to one end of the blocking member (134) away from the magnetic suction plate (133). The shape of the blocking member (135) is as follows. The size is adapted to the permeation tank (131). The end of the shield (135) near the magnetic plate (133) is rounded. The front and rear ends of the abutment (134) are fixedly connected to the side plate (136). The end of the side plate (136) away from the magnetic plate (133) is fixedly connected to the first elastic element (137). The first elastic element (137) is specifically a spring. The other end of the first elastic element (137) is fixedly connected to the inner wall of the activity chamber (13).

8. The anti-contamination device with built-in filter cartridge for pasteurized pipettes used for biological sample processing according to claim 1, characterized in that: The control center (30) includes a drive and force control module, a process and logic module, a user interaction module and an advanced function integration module, which are electrically connected to each other.

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