Peripheral blood immune cell centrifugal separation device

Abstract

The utility model relates to the technical field of cell separation, in particular to a peripheral blood immune cell centrifugal separation device which comprises a treatment box, a motor is fixedly connected to the bottom end in the treatment box, a second supporting disc is fixedly connected to the output shaft end of the motor, and four evenly-distributed mounting grooves are formed in the upper end face of the second supporting disc. The upper end of the second supporting disc is fixedly connected with a fixing rod, the upper end of the fixing rod is fixedly connected with a first supporting disc, the upper ends of the first supporting disc and the second supporting disc are connected with extrusion plates through reciprocating mechanisms, the two reciprocating mechanisms are fixedly connected through a rotating rod, and the upper end of the rotating rod is fixedly connected with a handle. According to the utility model, the test tubes with different diameters can be stably and firmly positioned in the mounting grooves, so that the test tubes with different specifications can be stably mounted in the first supporting disc and the second supporting disc, and the immune cell separation work is carried out on peripheral blood in the test tubes with different specifications.

Description

Technical Field

[0001] The utility model relates to the technical field of cell separation, in particular to a peripheral blood immune cell centrifugal separation device. Background Art

[0002] Human immune cells refer to cells that participate in or are related to immune responses, including lymphocytes, dendritic cells, monocytes, macrophages, granulocytes, mast cells, etc.; various immune cells play important roles in the human body. Mononuclear cells separated from the blood include: T cells, B cells, NK cells and DC cells, etc., which are the initial cell source in immune cell therapy. With age, the activity of immune cells in the body will also decrease. Therefore, freezing immune cells with high activity in a young age can provide a good guarantee for the treatment of tumor-related diseases in the future. Therefore, the separation and freezing of immune cells is of great significance.

[0003] At present, cell separation equipment is needed to separate peripheral blood immune cells. Before separating the cells, the extracted peripheral blood needs to be stored in a test tube, and the test tube is installed in the cell separation equipment. For test tubes, test tubes of different specifications have different diameters. In order to ensure that test tubes of different specifications can be firmly placed in the cell separation equipment for centrifugal separation operations, it is necessary to design a peripheral blood immune cell centrifugal separation device. Utility Model Content

[0004] The utility model aims to solve the shortcomings in the prior art and provides a peripheral blood immune cell centrifugal separation device.

[0005] In order to achieve the above-mentioned purpose, the utility model adopts the following technical scheme: a peripheral blood immune cell centrifugal separation device comprises a processing box, the bottom end of the processing box is fixedly connected to a motor, the output shaft end of the motor is fixedly connected to a second support plate, the upper end surface of the second support plate is provided with four evenly distributed mounting grooves, the upper end of the second support plate is fixedly connected to a fixing rod, the upper end of the fixing rod is fixedly connected to a first support plate, the upper end surface of the first support plate is provided with four evenly distributed mounting holes, the multiple mounting holes are respectively located at the upper ends of the corresponding multiple mounting grooves, the upper ends of the first support plate and the second support plate are connected to an extrusion plate through a reciprocating mechanism, the two reciprocating mechanisms are fixedly connected by a rotating rod, the rotating rod passes through the first support plate and is rotatably connected to the first support plate, and the upper end of the rotating rod is fixedly connected to a handle.

[0006] Furthermore, a support wheel is fixedly connected to the lower end of the second support plate, and a plurality of the support wheels are provided and evenly distributed around the motor.

[0007] Further, the reciprocating mechanism includes a disc, a fixed shaft, a connecting rod and a movable plate, the upper ends of the first support disc and the second support disc are rotatably connected with a disc, the two discs are fixedly connected by a rotating rod, the upper ends of the two discs are fixedly connected with four evenly distributed fixed shafts, the circumferential surface of each of the fixed shafts is rotatably connected with a connecting rod, the upper ends of the first support disc and the second support disc are slidably connected with a plurality of movable plates, the movable plate is fixedly connected to the extrusion plate, the plurality of movable plates are respectively located at the lower ends of a plurality of connecting rods, and the movable plates are rotatably connected to the connecting rods.

[0008] Furthermore, four evenly distributed fixing cylinders are fixedly connected to the lower end of the first supporting plate, and the fixing cylinders are located directly below the mounting holes. Flexible sleeves are fixedly installed on the inner walls of the fixing cylinders, mounting grooves and mounting holes, and a flexible pad is fixedly connected to one end of the extrusion plate away from the movable plate.

[0009] Furthermore, the upper ends of the first support plate and the second support plate are fixedly connected with guide rods, the guide rods penetrate the movable plate, and the movable plate is slidably connected to the guide rods.

[0010] Furthermore, a plurality of evenly distributed threaded holes are provided on the upper end surface of the upper disk, and fasteners are connected to the internal threads of the threaded holes. A plurality of evenly distributed slots are provided on the upper end surface of the first support disk, and the diameter of the slots is the same as the diameter of the threaded barrel.

[0011] The utility model has the following beneficial effects:

[0012] 1. Compared with the prior art, the peripheral blood immune cell centrifugal separation device can drive the extrusion plate to move toward or away from the rotating rod by controlling the operation of the reciprocating mechanism, so that test tubes of different diameters can be stably and firmly located in the installation groove, so that test tubes of different specifications can be stably installed in the first support plate and the second support plate, thereby performing immune cell separation work on the peripheral blood in the test tubes of different specifications.

[0013] 2. Compared with the prior art, this peripheral blood immune cell centrifugal separation device utilizes a fastener to be inserted into a corresponding slot along a threaded groove, so that the disc can be stably located at the upper end of the first support disc and will not rotate arbitrarily, thereby enabling the extrusion plate to stably squeeze the test tube and allow the test tube to be stably located in the mounting groove, so as to facilitate the subsequent smooth immune cell separation operation. BRIEF DESCRIPTION OF THE DRAWINGS

[0014] Figure 1 This is a schematic diagram of the overall structure of a peripheral blood immune cell centrifugal separation device proposed by the utility model;

[0015] Figure 2This is a schematic diagram of the top view of the peripheral blood immune cell centrifugal separation device proposed by the utility model after the disc and the fastener are separated;

[0016] Figure 3 This is a schematic diagram of the structure of a peripheral blood immune cell centrifugal separation device proposed by the utility model, with the processing box hidden;

[0017] Figure 4 The structure diagram of a peripheral blood immune cell centrifugal separation device proposed by the utility model is shown in the front view after partial section;

[0018] Figure 5 A peripheral blood immune cell centrifugal separation device proposed by the utility model Figure 4 Schematic diagram of the enlarged structure of A.

[0019] Legend:

[0020] 1. Processing box; 2. First support plate; 3. Second support plate; 4. Support wheel; 5. Motor; 6. Fixed rod; 7. Fixed cylinder; 8. Mounting groove; 9. Mounting hole; 10. Moving plate; 11. Connecting rod; 12. Handle; 13. Guide rod; 14. Disc; 15. Fastener; 16. Rotating rod; 17. Fixed shaft; 18. Slot; 19. Threaded hole; 20. Extrusion plate. DETAILED DESCRIPTION

[0021] Reference Figure 1-5The utility model provides a peripheral blood immune cell centrifugal separation device, including a processing box 1, a motor 5 is fixedly connected to the bottom end of the processing box 1, and a second support plate 3 is fixedly connected to the output shaft end of the motor 5. The upper end surface of the second support plate 3 is provided with four evenly distributed mounting grooves 8, and the upper end of the second support plate 3 is fixedly connected to a fixing rod 6, and the upper end of the fixing rod 6 is fixedly connected to the first support plate 2. The upper end surface of the first support plate 2 is provided with four evenly distributed mounting holes 9, and the plurality of mounting holes 9 are respectively located at the upper ends of the corresponding plurality of mounting grooves 8. The upper ends of the first support plate 2 and the second support plate 3 are both connected to an extrusion plate 20 through a reciprocating mechanism, and the two reciprocating mechanisms are fixedly connected by a rotating rod 16, which passes through the first support plate 2 and is rotatably connected to the first support plate 2, and the upper end of the rotating rod 16 is fixedly connected to a handle 12. During operation, peripheral blood is placed in the test tube, and then the position of the squeezing plate 20 is adjusted according to the diameter of the test tube. At this time, the rotating handle 12 can drive the rotating rod 16 to rotate, and then under the action of the reciprocating mechanism, the squeezing plate 20 is driven to move in the direction close to the rotating rod 16, until the squeezing plate 20 moves to a suitable position, the test tube can be loaded into the mounting groove 8 along the mounting hole 9, and then the rotating rod 16 is rotated in the opposite direction. Under the action of the reciprocating mechanism, the squeezing plate 20 is moved in the direction away from the rotating rod 16 until the squeezing plate 20 tightly squeezes the test tube. Since the upper ends of the first support plate 2 and the second support plate 3 are both provided with squeezing plates 20, and with the cooperation of the mounting hole 9 and the mounting groove 8, the test tube can be more stably located in the mounting groove 8, and then the motor 5 can be controlled to drive the second support plate 3 to rotate. At this time, with the cooperation of the fixed rod 6, the first support plate 2 is driven to rotate, and the test tube is driven to rotate by the first support plate 2 and the second support plate 3, thereby realizing the centrifugal separation of immune cells inside the peripheral blood.

[0022] Further, the lower end of the second support plate 3 is fixedly connected with a support wheel 4, and a plurality of support wheels 4 are provided and evenly distributed around the motor 5. During operation, under the action of the support wheel 4, the second support plate 3 can be rotated more smoothly.

[0023] Furthermore, the reciprocating mechanism includes a disc 14, a fixed shaft 17, a connecting rod 11 and a movable plate 10. The upper ends of the first support disc 2 and the second support disc 3 are rotatably connected to the disc 14, and the two discs 14 are fixedly connected by a rotating rod 16. The upper ends of the two discs 14 are fixedly connected to four evenly distributed fixed shafts 17, and the circumferential surface of each fixed shaft 17 is rotatably connected to the connecting rod 11. The upper ends of the first support disc 2 and the second support disc 3 are slidably connected to multiple movable plates 10, and the movable plate 10 is fixedly connected to the extrusion plate 20. The multiple movable plates 10 are respectively located at the lower ends of the multiple connecting rods 11, and the movable plates 10 are rotatably connected to the connecting rods 11. During operation, the rotation of the rotating rod 16 can simultaneously drive the two discs 14 to rotate, thereby driving the fixed shaft 17 to rotate, and then driving the connecting rod 11 to rotate. At this time, with the cooperation of the movable plate 10, the squeezing plate 20 is pulled toward or moves in the direction of the rotating rod 16, so that the position of the squeezing plate 20 can be adjusted, so that the squeezing plate 20 can clamp and fix test tubes of different diameters.

[0024] Furthermore, four evenly distributed fixing tubes 7 are fixedly connected to the lower end of the first support plate 2, and the fixing tubes 7 are located at the lower end of the mounting hole 9. Flexible sleeves are fixedly installed on the inner walls of the fixing tubes 7, the mounting grooves 8 and the mounting hole 9, and a flexible pad is fixedly connected to one end of the extrusion plate 20 away from the moving plate 10. During operation, the mutual cooperation of the flexible pad and the flexible sleeve can reduce the probability of test tube damage.

[0025] Furthermore, the upper ends of the first support plate 2 and the second support plate 3 are fixedly connected with guide rods 13, the guide rods 13 penetrate the movable plate 10, and the movable plate 10 is slidably connected to the guide rods 13. During operation, under the action of the guide rods 13, the movable plate 10 can be moved more stably.

[0026] Furthermore, a plurality of evenly distributed threaded holes 19 are provided on the upper end surface of the upper disk 14, and a fastener 15 is connected to the inner thread of the threaded hole 19, and a plurality of evenly distributed slots 18 are provided on the upper end surface of the first support disk 2, and the diameter of the slot 18 is the same as the diameter of the threaded barrel. During operation, when the disk 14 is rotated to a suitable position, the fastener 15 is inserted into the corresponding slot 18 along the threaded groove, so that the disk 14 can be stably located at the upper end of the first support disk 2 and will not rotate arbitrarily, so that the squeezing plate 20 can stably squeeze the test tube and the test tube can be stably located in the mounting groove 8, so as to facilitate the subsequent smooth immune cell separation operation.

[0027] Finally, it should be noted that the above are only preferred embodiments of the present invention and are not intended to limit the present invention. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art can still modify the technical solutions described in the aforementioned embodiments or make equivalent substitutions for some of the technical features therein. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the protection scope of the present invention.

Claims

1. A peripheral blood immune cell centrifugal separation device, comprising a processing box (1), characterized in that: The bottom end of the processing box (1) is fixedly connected to a motor (5), the output shaft end of the motor (5) is fixedly connected to a second support plate (3), the upper end surface of the second support plate (3) is provided with four evenly distributed mounting grooves (8), the upper end of the second support plate (3) is fixedly connected to a fixing rod (6), the upper end of the fixing rod (6) is fixedly connected to the first support plate (2), the upper end surface of the first support plate (2) is provided with four evenly distributed mounting holes (9), the plurality of mounting holes (9) are respectively located at the upper ends of the corresponding plurality of mounting grooves (8), the upper ends of the first support plate (2) and the second support plate (3) are both connected to an extrusion plate (20) via a reciprocating mechanism, the two reciprocating mechanisms are fixedly connected via a rotating rod (16), the rotating rod (16) passes through the first support plate (2) and is rotatably connected to the first support plate (2), and the upper end of the rotating rod (16) is fixedly connected to a handle (12).

2. A peripheral blood immune cell centrifugal separation device according to claim 1, characterized in that: The lower end of the second support plate (3) is fixedly connected to a support wheel (4), and a plurality of the support wheels (4) are provided and are evenly distributed around the motor (5).

3. A peripheral blood immune cell centrifugal separation device according to claim 1, characterized in that: The reciprocating mechanism comprises a disc (14), a fixed shaft (17), a connecting rod (11) and a movable plate (10); the upper ends of the first supporting disc (2) and the second supporting disc (3) are both rotatably connected with the disc (14); the two discs (14) are fixedly connected via a rotating rod (16); the upper ends of the two discs (14) are both fixedly connected with four evenly distributed fixed shafts (17); the circumferential surface of each fixed shaft (17) is rotatably connected with a connecting rod (11); the upper ends of the first supporting disc (2) and the second supporting disc (3) are both slidably connected with a plurality of movable plates (10); the movable plates (10) are fixedly connected with a pressing plate (20); the plurality of movable plates (10) are respectively located at the lower ends of a plurality of connecting rods (11), and the movable plates (10) are rotatably connected with the connecting rods (11).

4. A peripheral blood immune cell centrifugal separation device according to claim 1, characterized in that: The lower end of the first support plate (2) is fixedly connected to four evenly distributed fixing cylinders (7), and the fixing cylinders (7) are located directly below the mounting holes (9). Flexible sleeves are fixedly installed on the inner walls of the fixing cylinders (7), the mounting grooves (8) and the mounting holes (9), and a flexible pad is fixedly connected to one end of the extrusion plate (20) away from the movable plate (10).

5. The peripheral blood immune cell centrifugal separation device according to claim 1, characterized in that: The upper ends of the first support plate (2) and the second support plate (3) are both fixedly connected with guide rods (13), the guide rods (13) penetrate the movable plate (10), and the movable plate (10) is slidably connected to the guide rods (13).

6. The peripheral blood immune cell centrifugal separation device according to claim 1, characterized in that: The upper end surface of the upper circular disc (14) is provided with a plurality of evenly distributed threaded holes (19), the internal threads of the threaded holes (19) are connected with fasteners (15), and the upper end surface of the first supporting disc (2) is provided with a plurality of evenly distributed slots (18), the diameter of the slots (18) being the same as the diameter of the threaded barrel.

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