Anaerobic canning device for liquid culture medium

Abstract

The utility model relates to the technical field of culture medium canning storage, in particular to a liquid culture medium anaerobic canning device which exhausts air in a liquid containing bottle, avoids oxidation of oxygen in the air and can be stored for a long time. Comprising a storage tank, a liquid containing bottle, a sealing assembly, a cooling assembly and a deoxidizing assembly, the liquid containing bottle is located in the storage tank, the sealing assembly is installed at the top of the storage tank, the sealing assembly comprises a top cover, two fixing plates, two screw rods, two pulling plates, two tensioning blocks, a sealing sleeve and a sealing plug, and the top of the liquid containing bottle is sealed; the cooling assembly is installed at the bottom of the storage tank, the deoxidizing assembly is installed on the rear side wall of the storage tank, and the output end of the deoxidizing assembly communicates with the interior of the liquid containing bottle.

Description

Technical Field

[0001] The utility model relates to the technical field of culture medium canning and storage, in particular to an anaerobic canning device for liquid culture medium. Background Art

[0002] Culture medium is an artificially prepared nutrient for the growth and maintenance of microorganisms, plant tissues and animal tissues. It generally contains carbohydrates, nitrogen-containing substances, inorganic salts, vitamins and water. Due to the different raw materials, the use requirements of culture medium are different, and the storage and preservation are slightly different. Generally, culture medium is easily contaminated by bacteria or decomposed and deteriorated after being heated and absorbing moisture. Therefore, general culture medium must be stored in a moisture-proof, light-proof and cool place. For some culture media that need to be strictly sterilized, they must be placed in a refrigerator at 3-6℃ for long-term storage. Prior art announcement number CN207826967U proposes a canned storage device for culture medium, including a storage tank body, a culture medium storage test tube fixedly installed in the inner cavity of the storage tank body, a test tube plug inserted and installed at the upper end of the inner cavity of the culture medium storage test tube, a liquid nitrogen cavity opened in the inner cavity wall of the storage tank body, a bronchial cavity fixedly installed at the upper end of the liquid nitrogen cavity, a one-way air intake valve fixedly installed at the lower left end of the liquid nitrogen cavity, a valve cover fixedly installed at the right end of the one-way air intake valve by thread, a tank cover fixedly installed at the upper end of the storage tank body by thread, and a top groove opened on the lower end surface of the tank cover. However, anaerobic bacteria must grow in an oxygen-free environment, and it is not convenient to discharge oxygen in the prior art, and it is not very applicable to situations where the degree of anaerobicity is required to be high. Utility Model Content

[0003] In order to solve the above technical problems, the utility model provides an anaerobic canning device for liquid culture medium which can discharge the air inside a liquid bottle and avoid the oxidation effect of oxygen in the air and can store the liquid culture medium for a long time.

[0004] The utility model discloses an anaerobic canning device for liquid culture medium, comprising a storage tank, a liquid filling bottle, a sealing component, a cooling component and a deoxygenating component. The liquid filling bottle is located inside the storage tank, the sealing component is installed on the top of the storage tank to seal the top of the liquid filling bottle, the cooling component is installed on the bottom of the storage tank, the deoxygenating component is installed on the rear side wall of the storage tank, and the output end of the deoxygenating component is connected with the inside of the liquid filling bottle. After the culture medium is added into the liquid filling bottle, the inside of the liquid filling bottle is sealed by the sealing component, and the air inside the liquid filling bottle is discharged by the deoxygenating component to avoid the oxidation effect of oxygen in the air. The prepared culture medium has no oxidation layer and can be stored for a long time. The temperature inside the liquid filling bottle can be reduced by the cooling component to extend the storage time of the culture medium.

[0005] Preferably, the sealing assembly includes a top cover, two fixed plates, two screws, two pull plates, two tensioning blocks, a sealing sleeve and a sealing plug, the two fixed plates are symmetrically installed on the left and right ends of the outer wall of the storage tank, the two screws are rotatably installed on the fixed plates, the top cover is located above the storage tank, the left and right ends of the top cover are fixedly installed with pull plates, the top of the pull plates is fixedly installed with tensioning blocks, the middle part of the tensioning blocks is screwed on the outer wall of the screws, the bottom of the top cover is installed with a sealing sleeve that matches the top of the storage tank, and the middle part of the sealing sleeve is fixedly connected with a sealing plug that matches the top of the liquid bottle; after the culture medium is added to the liquid bottle, the top cover is installed on the top of the storage tank, the interior of the storage tank is sealed by the sealing sleeve, and at the same time, the sealing plug is driven to contact with the top of the liquid bottle to seal the interior of the liquid bottle, the screw is rotated to cooperate with the tensioning block, so that the pull plate and the fixed plate are close to each other, so that the top cover is tightly fixed to the top of the storage tank, thereby improving the sealing effect.

[0006] Preferably, the deoxygenation assembly includes a support seat, a spring, a pushing seat, a high-purity nitrogen cylinder, a top frame, a connecting head, a telescopic tube, an air inlet pipe, a solenoid valve and an air outlet pipe. The support seat is fixedly mounted on the rear end side wall of the storage tank, the pushing seat is slidably mounted inside the support seat, the spring is fixedly mounted on the inner bottom end of the support seat, the top of the spring is connected to the bottom of the pushing seat, the bottom of the high-purity nitrogen cylinder is in contact with the top of the pushing seat, the top frame is fixedly mounted on the upper part of the rear side wall of the storage tank, the connecting head is fixedly mounted on the lower part of the top frame, the high-purity nitrogen cylinder is inserted into the connecting head, the output end of the connecting head is connected to the telescopic tube, and the output end of the telescopic tube is connected to the input end of the air inlet pipe , an air inlet pipe passes through the top cover and the sealing plug and extends into the upper end of the liquid bottle, an electromagnetic valve is installed on the air inlet pipe, an air outlet pipe passes through the top cover and the sealing plug and extends into the lower side of the liquid bottle, and an electromagnetic valve is arranged on the air outlet pipe; when in use, the high-purity nitrogen cylinder is plugged into the connector, the high-purity nitrogen cylinder is pushed and fixed by a spring and a pushing seat, the electromagnetic valve is opened, and nitrogen is input into the liquid bottle through the telescopic tube and the air inlet pipe. Since nitrogen is lighter than air, the input end of the air outlet pipe is located at the lower part of the liquid bottle, which is conducive to fully exhausting the air in the liquid bottle and avoiding the oxidation of oxygen in the air. The prepared culture medium has no oxide layer and can be stored for a long time.

[0007] Preferably, the cooling component includes a semiconductor refrigeration plate, a cylinder, a plurality of first support rods, a fan, a temperature detector and a controller; the semiconductor refrigeration plate is mounted on the bottom end of the storage tank; the cylinder is sleeved on the bottom end of the storage tank outside the semiconductor refrigeration plate; the fan is fixedly mounted on the lower end of the inner wall of the cylinder through a plurality of first support rods; the temperature detector is fixedly mounted on the inner wall of the storage tank; an insulation layer is provided on the inner wall of the storage tank; the controller is fixedly mounted on the top of the top cover; the semiconductor refrigeration plate, the fan and the temperature detector are electrically connected to the controller; the interior of the storage tank is cooled by the semiconductor refrigeration plate and the fan; the temperature inside the storage tank is detected by the temperature detector; the device is conveniently controlled by the controller.

[0008] Preferably, the output end of the air outlet pipe is connected to a nitrogen detector, and the nitrogen detector and the solenoid valve are electrically connected to the controller; the discharged air is detected by the nitrogen detector, and when the discharged nitrogen content becomes high, the solenoid valve is controlled to close to ensure that the air is discharged cleanly and improve practicality.

[0009] Preferably, a filter is fixedly installed on the lower part of the outer wall of the cylinder, and a protective fence is connected to the bottom end of the cylinder; the cylinder facilitates heat dissipation to the outside, and the protective fence protects the fan to prevent the entry of debris, thereby increasing the service life.

[0010] Preferably, a plurality of support rods are evenly and fixedly connected to the bottom of the storage tank, and a support ring base is fixedly connected to the bottom of the support rods; the storage tank is supported by the support rods and the support ring base to ensure stability after placement.

[0011] Compared with the prior art, the utility model has the following beneficial effects: after the culture medium is added into the liquid bottle, the inside of the liquid bottle is sealed by the sealing component, and the air inside the liquid bottle is discharged by the deoxygenation component to avoid the oxidation effect of oxygen in the air. The prepared culture medium has no oxidation layer and can be stored for a long time. The temperature inside the liquid bottle can be lowered by the cooling component to extend the shelf life of the culture medium. BRIEF DESCRIPTION OF THE DRAWINGS

[0012] Figure 1 It is a structural schematic diagram of the utility model;

[0013] Figure 2 It is a schematic diagram of the axonometric structure of the utility model;

[0014] Figure 3 It is a right view structural schematic diagram of the utility model;

[0015] Figure 4 It is a front cross-sectional structural schematic diagram of the utility model;

[0016] Figure 5 It is a left-side cross-sectional structural schematic diagram of the utility model;

[0017] Markings in the attached drawings: 1. Storage tank; 2. Liquid bottle; 3. Top cover; 4. Fixed plate; 5. Screw; 6. Pull plate; 7. Tightening block; 8. Sealing sleeve; 9. Sealing plug; 10. Support seat; 11. Spring; 12. Tightening seat; 13. High-purity nitrogen cylinder; 14. Top frame; 15. Connector; 16. Telescopic tube; 17. Inlet pipe; 18. Solenoid valve; 19. Outlet pipe; 20. Nitrogen detector; 21. Semiconductor refrigeration plate; 22. Cylinder; 23. First support rod; 24. Fan; 25. Temperature detector; 26. Controller; 27. Filter; 28. Protective fence; 29. ​​Support rod; 30. Support ring base. DETAILED DESCRIPTION

[0018] In order to facilitate the understanding of the utility model, the utility model will be described more comprehensively below with reference to the relevant drawings. The utility model can be implemented in many different forms and is not limited to the embodiments described herein. On the contrary, the purpose of providing these embodiments is to make the disclosure of the utility model more thorough and comprehensive.

[0019] Example 1

[0020] like Figure 1 , Figure 3 , Figure 4 and Figure 5 As shown, the liquid bottle 2 is located inside the storage tank 1, two fixing plates 4 are symmetrically installed on the left and right ends of the outer wall of the storage tank 1, two screws 5 are rotatably installed on the fixing plates 4, the top cover 3 is located above the storage tank 1, and the left and right ends of the top cover 3 are fixedly installed with pull plates 6, the top of the pull plate 6 is fixedly installed with a tightening block 7, the middle part of the tightening block 7 is screwed on the outer wall of the screw 5, the bottom of the top cover 3 is installed with a sealing sleeve 8 that matches the top of the storage tank 1, the middle part of the sealing sleeve 8 is fixedly connected with a sealing plug 9 that matches the top of the liquid bottle 2, the support seat 10 is fixedly installed on the rear end side wall of the storage tank 1, the pushing seat 12 is slidably installed inside the support seat 10, and the spring 11 is fixedly installed on the support seat 10. At the inner bottom end, the top of the spring 11 is connected to the bottom of the push seat 12, the bottom of the high-purity nitrogen cylinder 13 is in contact with the top of the push seat 12, the top frame 14 is fixedly installed on the upper part of the rear side wall of the storage tank 1, the connector 15 is fixedly installed at the lower part of the top frame 14, the high-purity nitrogen cylinder 13 is inserted on the connector 15, the output end of the connector 15 is connected to the telescopic tube 16, the output end of the telescopic tube 16 is connected to the input end of the air inlet pipe 17, the air inlet pipe 17 passes through the top cover 3 and the sealing plug 9 and extends into the inner upper end of the liquid bottle 2, the air inlet pipe 17 is installed with a solenoid valve 18, the air outlet pipe 19 passes through the top cover 3 and the sealing plug 9 and extends into the inner lower side of the liquid bottle 2, and the air outlet pipe 19 is provided with a solenoid valve 18;

[0021] After the culture medium is added to the liquid bottle 2, the top cover 3 is installed on the top of the storage tank 1, and the interior of the storage tank 1 is sealed by the sealing sleeve 8. At the same time, the sealing plug 9 is driven to contact the top of the liquid bottle 2 to seal the interior of the liquid bottle 2, and the screw 5 is rotated to cooperate with the tightening block 7 to make the pulling plate 6 and the fixing plate 4 close to each other, so that the top cover 3 is tightly fixed on the top of the storage tank 1 to improve the sealing effect, and the high-purity nitrogen cylinder 13 is plugged into the connector 15, and the high-purity nitrogen cylinder 13 is pushed and fixed by the spring 11 and the pushing seat 12, and the solenoid valve 18 is opened to allow nitrogen to be input into the liquid bottle 2 through the telescopic tube 16 and the air inlet pipe 17. Since nitrogen is lighter than air, the input end of the air outlet pipe 19 is located at the lower part of the liquid bottle 2, which is conducive to fully exhausting the air in the liquid bottle 2 and avoiding the oxidation of oxygen in the air. The prepared culture medium has no oxide layer and can be stored for a long time.

[0022] Example 2

[0023] like Figures 2 to 4 As shown, on the basis of Example 1, it also includes a semiconductor refrigeration plate 21 installed at the bottom end of the storage tank 1, a cylinder 22 is sleeved on the bottom end of the storage tank 1 outside the semiconductor refrigeration plate 21, a fan 24 is fixedly installed on the lower end of the inner wall of the cylinder 22 through a plurality of first support rods 23, a temperature detector 25 is fixedly installed on the inner wall of the storage tank 1, an insulation layer is provided on the inner wall of the storage tank 1, a controller 26 is fixedly installed on the top of the top cover 3, the semiconductor refrigeration plate 21, the fan 24 and the temperature detector 25 are electrically connected to the controller 26, the output end of the air outlet pipe 19 is connected to the nitrogen detector 20, the nitrogen detector 20 and the solenoid valve 18 are electrically connected to the controller 26, a filter screen 27 is fixedly installed on the lower part of the outer wall of the cylinder 22, a protective fence 28 is connected to the bottom end of the cylinder 22, a plurality of support rods 29 are evenly fixedly connected to the bottom of the storage tank 1, and a support ring base 30 is fixedly connected to the bottom of the support rods 29;

[0024] The interior of the storage tank 1 is cooled by the semiconductor refrigeration plate 21 and the fan 24, the temperature detector 25 detects the temperature inside the storage tank 1, the controller 26 facilitates the control of the equipment, the exhaust air is detected by the nitrogen detector 20, when the exhaust nitrogen content becomes high, the solenoid valve 18 is controlled to close to ensure that the air is discharged cleanly and improve practicality, the cylinder 22 facilitates the dissipation of heat to the outside, and the protective fence 28 protects the fan 24 to prevent the entry of debris and increase the service life, and the storage tank 1 is supported by the support rod 29 and the support ring base 30 to ensure stability after placement.

[0025] like Figures 1 to 5As shown, the utility model is an anaerobic canning device for liquid culture medium. When it is working, after the culture medium is added to the liquid bottle 2, the top cover 3 is installed on the top of the storage tank 1, and the inside of the storage tank 1 is sealed by the sealing sleeve 8. At the same time, the sealing plug 9 is driven to contact with the top of the liquid bottle 2 to seal the inside of the liquid bottle 2. The screw 5 is rotated to cooperate with the tension block 7 to make the pull plate 6 and the fixing plate 4 close to each other, so that the top cover 3 is tightly fixed on the top of the storage tank 1, and the high-purity nitrogen cylinder 13 is plugged into the connector 15. The high-purity nitrogen cylinder 13 is pushed and fixed by the spring 11 and the pushing seat 12, and the solenoid valve 18 is opened to allow nitrogen to be transported through the telescopic tube 16 and the air inlet pipe 17. The nitrogen gas enters the liquid bottle 2. Since nitrogen is lighter than air, the input end of the air outlet pipe 19 is located at the lower part of the liquid bottle 2, which is conducive to fully exhausting the air in the liquid bottle 2. The discharged air is detected by the nitrogen detector 20. When the discharged nitrogen content becomes high, the solenoid valve 18 is controlled to be closed, and the interior of the storage tank 1 is cooled by the semiconductor refrigeration plate 21 and the fan 24. The temperature detector 25 detects the temperature inside the storage tank 1, and the cylinder 22 is used to facilitate outward heat dissipation. At the same time, the protective fence 28 protects the fan 24 to prevent the entry of debris. The storage tank 1 is supported by the support rod 29 and the support ring base 30 to ensure stability after placement.

[0026] The high-purity nitrogen cylinder 13, solenoid valve 18, nitrogen detector 20, semiconductor refrigeration plate 21, fan 24 and controller 26 of the liquid culture medium anaerobic canning device of the utility model are purchased on the market, and technicians in the industry only need to install and operate them according to the accompanying instruction manual, without the need for technical personnel in this field to make creative labor.

[0027] The above is only a preferred embodiment of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the technical principle of the present invention. These improvements and modifications should also be regarded as the protection scope of the present invention.

Claims

1. A liquid culture medium anaerobic canning device, characterized in that: The storage tank (1) comprises a storage tank (1), a liquid bottle (2), a sealing component, a cooling component and a deoxygenating component. The liquid bottle (2) is located inside the storage tank (1). The sealing component is installed on the top of the storage tank (1) to seal the top of the liquid bottle (2). The cooling component is installed on the bottom of the storage tank (1). The deoxygenating component is installed on the rear side wall of the storage tank (1), and the output end of the deoxygenating component is connected to the inside of the liquid bottle (2).

2. The liquid culture medium anaerobic canning device according to claim 1, characterized in that: The sealing assembly comprises a top cover (3), two fixed plates (4), two screw rods (5), two pull plates (6), two tension blocks (7), a sealing sleeve (8) and a sealing plug (9). The two fixed plates (4) are symmetrically mounted on the left and right ends of the outer wall of the storage tank (1). The two screw rods (5) are rotatably mounted on the fixed plates (4). The top cover (3) is located above the storage tank (1). The left and right ends of the top cover (3) are fixedly mounted with pull plates (6). The top of the pull plates (6) is fixedly mounted with a tension block (7). The middle part of the tension block (7) is screwed onto the outer wall of the screw rods (5). The bottom of the top cover (3) is mounted with a sealing sleeve (8) matched with the top of the storage tank (1). The middle part of the sealing sleeve (8) is fixedly connected with a sealing plug (9) matched with the top of the liquid bottle (2).

3. The liquid culture medium anaerobic canning device according to claim 2, characterized in that: The deoxidation assembly comprises a support seat (10), a spring (11), a push seat (12), a high-purity nitrogen cylinder (13), a top frame (14), a connector (15), a telescopic tube (16), an air inlet pipe (17), a solenoid valve (18) and an air outlet pipe (19). The support seat (10) is fixedly mounted on the rear end side wall of the storage tank (1), the push seat (12) is slidably mounted inside the support seat (10), the spring (11) is fixedly mounted on the inner bottom end of the support seat (10), the top of the spring (11) is connected to the bottom of the push seat (12), the bottom of the high-purity nitrogen cylinder (13) is in contact with the top of the push seat (12), and the top frame (14) is fixedly mounted on the rear end side wall of the storage tank (1). The connecting head (15) is installed on the upper part of the rear side wall of the storage tank (1), and the connecting head (15) is fixedly installed on the lower part of the top frame (14). The high-purity nitrogen cylinder (13) is inserted into the connecting head (15). The output end of the connecting head (15) is connected to the telescopic tube (16), and the output end of the telescopic tube (16) is connected to the input end of the air inlet pipe (17). The air inlet pipe (17) passes through the top cover (3) and the sealing plug (9) and extends into the upper end of the liquid bottle (2). The air inlet pipe (17) is installed with a solenoid valve (18). The air outlet pipe (19) passes through the top cover (3) and the sealing plug (9) and extends into the lower side of the liquid bottle (2). The air outlet pipe (19) is provided with a solenoid valve (18).

4. The liquid culture medium anaerobic canning device according to claim 1, characterized in that: The cooling component comprises a semiconductor refrigeration plate (21), a cylinder (22), a plurality of first support rods (23), a fan (24), a temperature detector (25) and a controller (26); the semiconductor refrigeration plate (21) is mounted on the bottom end of the storage tank (1); the cylinder (22) is sleeved on the bottom end of the storage tank (1) outside the semiconductor refrigeration plate (21); the fan (24) is fixedly mounted on the lower end of the inner wall of the cylinder (22) through the plurality of first support rods (23); the temperature detector (25) is fixedly mounted on the inner wall of the storage tank (1); a heat-insulating layer is arranged on the inner wall of the storage tank (1); the controller (26) is fixedly mounted on the top of the top cover (3); the semiconductor refrigeration plate (21), the fan (24) and the temperature detector (25) are electrically connected to the controller (26).

5. The liquid culture medium anaerobic canning device according to claim 3, characterized in that: The output end of the gas outlet pipe (19) is connected to a nitrogen detector (20), and the nitrogen detector (20) and the solenoid valve (18) are electrically connected to a controller (26).

6. The liquid culture medium anaerobic canning device according to claim 4, characterized in that: A filter screen (27) is fixedly mounted on the lower portion of the outer wall of the cylinder (22), and a protective fence (28) is connected to the bottom end of the cylinder (22).

7. The liquid culture medium anaerobic canning device according to claim 1, characterized in that: A plurality of support rods (29) are evenly and fixedly connected to the bottom of the storage tank (1), and a support ring base (30) is fixedly connected to the bottom of the support rods (29).

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