Device for rapidly separating soil bacillus

Abstract

The utility model relates to the field of soil bacillus separating devices, in particular to a device for quickly separating soil bacillus, and aims to solve the technical problems that a soil sample is rich in strains, bacillus is difficult to be independently enriched on a culture medium, and the separation is not thorough. According to the technical scheme, the device for rapidly separating the soil bacillus comprises a water bath tank, a dilution tank, a first-stage liquid outlet pipe, a heating rod, a liquid accumulation tank, a boss, a second-stage liquid outlet pipe and a coating assembly; according to the integrated soil bacillus separating device with the water bath heating function, samples are added and diluted, meanwhile, the samples are preheated, heat-labile strains in soil are inactivated, ten independent samples with the same amount can be separated out at a time through the liquid collecting tank with ten secondary liquid outlet pipes, and the separation efficiency is greatly improved. The separation efficiency of soil bacillus in a multi-sample control experiment is improved, and the problems that a soil sample is rich in multiple strains, bacillus is difficult to be independently enriched on a culture medium, and separation is not thorough are solved.

Description

Technical Field

[0001] The utility model relates to the field of soil bacillus separation devices, in particular to a device for rapidly separating soil bacillus. Background Art

[0002] Since soil has the nutrients, air and water required by microorganisms, it is extremely rich in bacterial samples. It is the place where microorganisms are most concentrated. Almost any desired strain can be isolated from the soil, so soil samples are often the preferred collection target. Bacillus is a microorganism that is abundant in the soil and has wide application value.

[0003] The isolation of Bacillus in soil is usually carried out by the dilution spread plate method, in which the soil sample is diluted and spread on the surface of the solid culture medium in the plate to form an isolated strain. However, the soil sample contains not only Bacillus, but also other bacterial species. During separation, other bacterial species will also be enriched on the culture medium, resulting in incomplete isolation of Bacillus.

[0004] Therefore, in view of the fact that the above-mentioned soil samples are rich in many bacterial species, Bacillus is difficult to enrich alone on the culture medium, and the separation is not thorough, a device for rapid separation of soil Bacillus based on a high-temperature inactivation method can be designed, and multiple equal amounts of soluble Bacillus samples can be separated at one time, which is conducive to rapid control experiments. Utility Model Content

[0005] In order to overcome the problem that soil samples are rich in many bacterial species, Bacillus is difficult to enrich alone on the culture medium and the separation is not complete.

[0006] The technical scheme of the utility model is: a device for quickly separating soil bacillus, comprising a support platform, a back frame, a support ring, a water bath, a dilution tank, a primary liquid outlet pipe, a heating rod, a liquid accumulation tank, a boss, a secondary liquid outlet pipe, and a coating component; a flat plate with a protein culture medium added is placed on the support platform, a back frame is fixedly connected to the upper side of the rear end of the support platform, a support ring located above the middle of the flat plate is fixedly connected to the upper end of the back frame, a water bath is plugged and installed in the support ring, sterile water and a soil sample are contained in the dilution tank, and the dilution tank is in the water bath The inner and lower end of the liquid storage tank is integrally connected with a primary liquid outlet pipe which passes through the lower end of the water bath tank. Six heating rods are installed in the water bath tank and surround the dilution tank. A liquid storage tank is arranged below the water bath tank. The lower end of the primary liquid outlet pipe is in the liquid storage tank. An upwardly raised boss is arranged on the bottom wall of the liquid storage tank. Ten secondary liquid outlet pipes are equidistantly arranged around the boss on the bottom wall of the liquid storage tank, and the upper end of the secondary liquid outlet pipe is 4cm-8cm above the bottom wall surface of the liquid storage tank. The boss is a hollow structure and a coating component for coating the sample liquid is installed inside.

[0007] Preferably, because Bacillus can produce spores and resist high heat, other non-heat-resistant microorganisms in the soil sample can be killed by an integrated soil Bacillus separation device with a water bath heating function. Then, the sample is discharged into the liquid accumulation tank through the dilution tank and is filled with Bacillus when collected, which is helpful for the enrichment and separation of Bacillus. By opening ten secondary liquid outlet pipes, ten independent samples of equal amounts can be separated at one time, and soil impurities can be prevented from entering the secondary liquid outlet pipes, and then each sample is coated on a different culture medium using a coating component.

[0008] Preferably, a manually opened and closed valve structure is installed on both the primary liquid outlet pipe and the secondary liquid outlet pipe, the heating rod is externally connected to the mains power supply and heats the water in the water bath to 100°C, the upper end of the boss is an arc-shaped protrusion structure, and the lower end of the primary liquid outlet pipe is above the boss. 10g of soil sample and 90ml of sterile water are put into the dilution tank, and the solution is stirred with a stirring rod to accelerate dissolution. At the same time, the heating rod heats the water in the water bath to 100°C to kill the heat-sensitive bacteria in the dilution tank and retain the Bacillus. The valve on the primary liquid outlet pipe is opened, and the inactivated sample passes through The first-level liquid outlet pipe drips onto the boss and slides down along the boss and is deposited at the bottom of the liquid accumulation tank. The water-insoluble soil impurities in the sample solution accumulate at the bottom of the liquid accumulation tank, and the aqueous solution is in the upper layer. The aqueous solution slowly rises and slowly flows into from the top of the second-level liquid outlet pipe. After all the solution is discharged from the dilution tank, the liquid level of the aqueous solution in the liquid accumulation tank is stable and flush with the top of the second-level liquid outlet pipe. The valve on the second-level liquid outlet pipe is opened, and ten test tubes are placed under each second-level liquid outlet pipe respectively. The aqueous solution in the second-level liquid outlet pipe flows out into the test tubes and is collected waiting for the isolation of Bacillus.

[0009] Preferably, the coating assembly includes a ferrule, a coating brush, and a rubber head; the ferrule is fixedly connected to the cavity at the bottom of the boss, the coating brush is in the cavity at the bottom of the boss, the upper end of the coating brush is fixedly connected to the rubber head, and the rubber head is plugged and fixed in the ferrule, and the coating brush is hidden in the cavity at the bottom of the boss by plugging and fixing the rubber head and the ferrule. After being pulled out, the sample in the test tube is dipped and coated on different culture medium plates.

[0010] Preferably, the outer wall of the water bath is fixedly connected to a ring beam above the supporting ring, and the lower end of the water bath is symmetrically fixedly connected to two suspension rods with external threads, on which screw rings are threaded. The supporting ring supports the ring beam to prevent the water bath from sliding off the supporting ring, and the suspension rod serves as a suspension and fixing structure of the liquid accumulation tank.

[0011] Preferably, a balance frame is fixedly connected to the left and right ends of the outer wall of the liquid accumulation tank, and a ring that is sleeved on the corresponding suspension rod is fixedly connected to the end of the balance frame. The screw ring is supported on the lower side of the ring, and the screw ring is screwed on the suspension rod, and the bottom of the ring is supported to prevent the ring from slipping. This installation method is also convenient for the assembly and disassembly of the liquid accumulation tank and the suspension rod.

[0012] Preferably, a back plate with a hollow structure is fixedly installed at the front end of the back frame, and the back plate is inclined forward and downward. An auxiliary light powered by an external AC power supply is installed at the front end of the back plate. The auxiliary light provides auxiliary lighting for the position of the liquid accumulation tank, making it convenient to operate the equipment and group the sample solutions at night.

[0013] Preferably, a blower powered by an external mains electricity is installed at the rear end of the back panel, and an air outlet connected to the air outlet channel of the blower is opened at the lower part of the front end of the back panel. The blower conveys wind power to one side of the liquid accumulation tank through the air outlet to accelerate the cooling of the aqueous solution sample and ensure that the temperature of the sample solution is below 25°C during coating to avoid melting of the culture medium.

[0014] Beneficial effects of the utility model:

[0015] 1. The integrated soil Bacillus separation device with water bath heating function can preheat the sample while adding sample dilution, quickly inactivate heat-sensitive bacteria in the soil, and help enrich and separate Bacillus. In addition, the liquid storage tank with ten secondary liquid outlet pipes can separate ten independent samples of equal volume at one time, without the need to separate samples one by one, thus improving the separation efficiency of soil Bacillus in multi-sample control experiments;

[0016] 2. Through the height difference between the boss in the liquid storage tank and the top of the secondary liquid outlet pipe and the bottom wall of the liquid storage tank, when separating the samples, the water-insoluble soil impurities can be deposited at the bottom of the liquid storage tank to prevent the impurities from entering the secondary liquid outlet pipe, which is conducive to the subsequent coating work;

[0017] 3. The blower on the back plate and the air outlet facing the liquid accumulation tank can be used to cool the discharged sample solution to avoid melting of the culture medium when the sample is coated with heat. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] Figure 1 The three-dimensional structure diagram of the device for rapid separation of soil Bacillus of the utility model is shown;

[0019] Figure 2 The schematic diagram of the three-dimensional structure of the support platform of the device for rapid separation of soil Bacillus of the utility model is shown;

[0020] Figure 3 Shown is a schematic diagram of the overall structure of the water bath tank of the device for rapid separation of soil Bacillus of the utility model;

[0021] Figure 4 Shown is a three-dimensional schematic diagram of the internal structure of the water bath tank of the device for rapid separation of soil Bacillus of the utility model;

[0022] Figure 5The three-dimensional structure diagram of the overall structure of the liquid storage tank of the device for rapid separation of soil Bacillus of the utility model is shown;

[0023] Figure 6 Shown is a three-dimensional schematic diagram of the internal structure of the liquid storage tank of the device for quickly separating soil Bacillus of the present invention.

[0024] Explanation of the reference numerals: 1. support platform; 2. back frame; 3. support ring; 4. water bath tank; 5. dilution tank; 6. primary liquid outlet pipe; 7. heating rod; 8. liquid accumulation tank; 9. boss; 10. secondary liquid outlet pipe; 12. ring beam; 13. suspension rod; 14. screw ring; 15. balance frame; 16. sleeve ring; 17. back plate; 18. auxiliary light; 19. blower; 20. air outlet; 1101. ferrule; 1102. coating brush; 1103. rubber head. DETAILED DESCRIPTION

[0025] The utility model is further described below in conjunction with the accompanying drawings and embodiments.

[0026] See also Figure 1-6 The utility model provides an embodiment: a device for quickly separating soil bacillus, comprising a support platform 1, a back frame 2, a support ring 3, a water bath tank 4, a dilution tank 5, a primary liquid outlet pipe 6, a heating rod 7, a liquid accumulation tank 8, a boss 9, a secondary liquid outlet pipe 10, and a coating component; a plate with protein culture medium added is placed on the support platform 1, a back frame 2 is fixedly connected to the upper side of the rear end of the support platform 1, a support ring 3 located above the middle of the plate is fixedly connected to the upper end of the back frame 2, a water bath tank 4 is plugged and installed in the support ring 3, a dilution tank 5 is filled with sterile water and a soil sample, the dilution tank 5 is in the water bath tank 4 and the lower end is integrally connected with a primary liquid outlet pipe 6 that passes through the lower end of the water bath tank 4, six heating rods 7 that surround the dilution tank 5 are installed in the water bath tank 4, a liquid accumulation tank 8 is arranged below the water bath tank 4, the lower end of the primary liquid outlet pipe 6 is in the liquid accumulation tank 8, and the liquid accumulation tank 8 A boss 9 that protrudes upward is provided on the bottom wall. Ten secondary liquid outlet pipes 10 are equidistantly arranged around the boss 9 on the bottom wall of the liquid storage tank 8, and the height from the upper end of the secondary liquid outlet pipe 10 to the bottom wall surface of the liquid storage tank 8 is 4cm-8cm. The boss 9 is a hollow structure and a coating component for coating the sample liquid is installed inside. Because Bacillus can produce spores and can resist high heat, other non-heat-resistant microorganisms in the soil sample can be killed by an integrated soil Bacillus separation device with a water bath heating function. Then the sample is discharged into the liquid storage tank 8 through the dilution tank 5 and is filled with Bacillus when collected, which is helpful for the enrichment and separation of Bacillus. Opening the ten secondary liquid outlet pipes 10 can separate ten independent samples of equal amount at one time, and prevent soil impurities from entering the secondary liquid outlet pipes 10, and then use the coating component to coat each sample on a different culture medium.

[0027] See also Figure 3-6In this embodiment, both the primary liquid outlet pipe 6 and the secondary liquid outlet pipe 10 are equipped with a manually opened and closed valve structure, the heating rod 7 is externally connected to the mains power supply and heats the water in the water bath tank 4 to 100°C, the upper end of the boss 9 is an arc-shaped protruding structure, and the lower end of the primary liquid outlet pipe 6 is above the boss 9. 10g of soil sample and 90ml of sterile water are put into the dilution tank 5. While the stirring rod is used to stir the solution to accelerate the dissolution, the heating rod 7 heats the water in the water bath tank 4 to 100°C, kills the heat-sensitive bacteria in the dilution tank 5, retains the Bacillus, opens the valve on the primary liquid outlet pipe 6, and the inactivated sample drips onto the boss 9 through the primary liquid outlet pipe 6 and slides along the boss 9 and is deposited at the bottom of the liquid accumulation tank 8. The water-insoluble soil impurities in the sample solution accumulate at the bottom of the liquid accumulation tank 8, and the water-soluble The liquid is in the upper layer, and the aqueous solution slowly rises and slowly flows into from the top of the secondary liquid outlet pipe 10. After the solution is completely discharged from the dilution tank 5, the liquid level of the aqueous solution in the liquid accumulation tank 8 is stable and flush with the top of the secondary liquid outlet pipe 10. Open the valve on the secondary liquid outlet pipe 10, and place ten test tubes under each secondary liquid outlet pipe 10 respectively. The aqueous solution in the secondary liquid outlet pipe 10 flows out into the test tubes and is collected to wait for the separation of Bacillus. The outer wall of the water bath tank 4 is fixedly connected with a ring beam 12 located above the supporting ring 3. The lower end of the water bath tank 4 is fixedly connected with two suspension rods 13 with external threads symmetrically on the left and right. The suspension rods 13 are threaded with screw rings 14. The supporting ring 3 supports the ring beam 12 to prevent the water bath tank 4 from sliding off the supporting ring 3. The suspension rod 13 serves as a suspended fixing structure of the liquid accumulation tank 8.

[0028] See also Figure 5-6 In this embodiment, the coating assembly includes a sleeve 1101, a coating brush 1102, and a rubber head 1103; the sleeve 1101 is fixedly connected to the cavity at the bottom of the boss 9, the coating brush 1102 is in the cavity at the bottom of the boss 9, the upper end of the coating brush 1102 is fixedly connected to the rubber head 1103, the rubber head 1103 is plugged and fixed to the sleeve 1101, and the coating brush 1102 is hidden in the empty space at the bottom of the boss 9 by plugging and fixing the rubber head 1103 with the sleeve 1101. The sample in the test tube is dipped into the cavity and spread on different culture medium plates after being pulled out. A balance frame 15 is fixedly connected to both ends of the outer wall of the liquid accumulation tank 8. The end of the balance frame 15 is fixedly connected to a ring 16 which is sleeved on the corresponding suspension rod 13. The screw ring 14 is supported on the lower side of the ring 16. The screw ring 14 is screwed on the suspension rod 13 and supports the bottom of the ring 16 to prevent it from slipping. This installation method is also convenient for the assembly and disassembly of the liquid accumulation tank 8 and the suspension rod 13.

[0029] See also Figure 2In this embodiment, a back plate 17 with a hollow structure is fixedly installed at the front end of the back frame 2. The back plate 17 is inclined forward and downward. An auxiliary light 18 powered by an external AC power supply is installed at the front end of the back plate 17. The auxiliary light 18 provides auxiliary lighting for the position of the liquid accumulation tank 8, which is convenient for operating the equipment and grouping the sample solutions at night. A blower 19 powered by an external AC power supply is installed at the rear end of the back plate 17. An outlet 20 connected to the air outlet channel of the blower 19 is provided at the lower part of the front end of the back plate 17. The blower 19 conveys air to one side of the liquid accumulation tank 8 through the outlet 20, accelerates the cooling of the aqueous solution sample, ensures that the sample solution temperature is below 25°C during coating, and avoids melting of the culture medium.

[0030] When working, the operator takes 10g of soil sample and 90ml of sterile water and puts them into the dilution tank 5, then adds 200ml of sterile water into the water bath tank 4, starts the heating rod 7 to heat the water in the water bath tank 4 to 100°C, and uses the stirring rod to stir the solution to accelerate the dissolution of the soil sample, kill the heat-sensitive bacteria in the dilution tank 5, and retain the Bacillus;

[0031] Then, the valve on the primary liquid outlet pipe 6 is opened, and the inactivated sample drips onto the boss 9 through the primary liquid outlet pipe 6. The water-insoluble soil impurities in the sample solution accumulate at the bottom of the liquid storage tank 8, and the aqueous solution is in the upper layer. The aqueous solution slowly rises and slowly flows into and fills the secondary liquid outlet pipe 10 from the top of the secondary liquid outlet pipe 10. Each secondary liquid outlet pipe 10 has 10 ml of aqueous solution sample. After all the solution is discharged from the dilution tank 5, the liquid level of the aqueous solution in the liquid storage tank 8 is stable and flush with the top of the secondary liquid outlet pipe 10.

[0032] At this time, the blower 19 sends air to the liquid storage tank 8 to accelerate the cooling of the aqueous solution sample, and then ten test tubes are placed under each secondary liquid outlet pipe 10, and the aqueous solution in the secondary liquid outlet pipe 10 flows out into the test tubes and is collected to wait for the separation of Bacillus;

[0033] Take a plate containing culture medium and place it on the support 1, then remove the coating brush 1102 from the sleeve 1101, dip the sample in the test tube and spread it on the culture medium plate, then replace another sample and spread it on another culture medium plate.

[0034] Through the above steps, by using an integrated soil Bacillus separation device with a water bath heating function, the sample can be preheated simultaneously while adding sample dilution, and the heat-intolerant bacteria in the soil can be quickly inactivated, which is helpful for the enrichment and separation of Bacillus. In addition, by using a liquid storage tank 8 with ten secondary liquid outlet pipes 10, ten equal independent samples can be separated at one time without the need to separate the samples one by one, thereby improving the soil Bacillus separation efficiency in multi-sample control experiments, so as to solve the problem that the soil sample is rich in many bacterial species, Bacillus is difficult to enrich alone on the culture medium, and the separation is not thorough.

[0035] The embodiments of the present invention are described in detail above in conjunction with the accompanying drawings, but the present invention is not limited to the above embodiments, and various changes can be made within the knowledge scope of those skilled in the art without departing from the purpose of the present invention.

Claims

1. A device for rapidly separating soil Bacillus, comprising a support platform (1) and a back frame (2); characterized in that: It also includes a supporting ring (3), a water bath tank (4), a dilution tank (5), a primary liquid outlet pipe (6), a heating rod (7), a liquid accumulation tank (8), a boss (9), a secondary liquid outlet pipe (10), and a coating component; a plate with a protein culture medium added is placed on the supporting platform (1), a back frame (2) is fixedly connected to the upper side of the rear end of the supporting platform (1), a supporting ring (3) located above the middle of the plate is fixedly connected to the upper end of the back frame (2), the water bath tank (4) is plugged into the supporting ring (3), the dilution tank (5) contains sterile water and soil samples, the dilution tank (5) is in the water bath tank (4) and the lower end is integrally connected to a tube extending from the water bath tank. (4) a first-level liquid outlet pipe (6) at the lower end, six heating rods (7) are installed in the water bath (4) and surround the dilution tank (5), a liquid storage tank (8) is arranged below the water bath (4), the lower end of the first-level liquid outlet pipe (6) is in the liquid storage tank (8), a boss (9) protruding upward is arranged on the bottom wall of the liquid storage tank (8), ten second-level liquid outlet pipes (10) are arranged equidistantly around the boss (9) on the bottom wall of the liquid storage tank (8), and the height of the upper end of the second-level liquid outlet pipe (10) from the bottom wall surface of the liquid storage tank (8) is 4 cm-8 cm, the boss (9) is a hollow structure and a coating component for coating sample liquid is installed inside.

2. A device for rapid separation of soil Bacillus according to claim 1, characterized in that: A manually opened and closed valve structure is installed on both the first-stage liquid outlet pipe (6) and the second-stage liquid outlet pipe (10). The heating rod (7) is externally connected to the mains power supply and heats the water in the water bath tank (4) to 100° C. The upper end of the boss (9) is an arc-shaped raised structure, and the lower end of the first-stage liquid outlet pipe (6) is located above the boss (9).

3. A device for rapid separation of soil Bacillus according to claim 1, characterized in that: The coating assembly comprises a ferrule (1101), a coating brush (1102), and a rubber head (1103); the ferrule (1101) is fixedly connected to the cavity at the bottom of the boss (9), the coating brush (1102) is in the cavity at the bottom of the boss (9), the upper end of the coating brush (1102) is fixedly connected to the rubber head (1103), and the rubber head (1103) is plugged and fixed to the ferrule (1101).

4. The device for rapid separation of soil Bacillus according to claim 1, characterized in that: The outer wall of the water bath tank (4) is fixedly connected with a ring beam (12) located above the supporting ring (3), and the lower end of the water bath tank (4) is symmetrically fixedly connected with two suspension rods (13) with external threads, and the suspension rods (13) are threadedly connected with a screw ring (14).

5. A device for rapid separation of soil Bacillus according to claim 4, characterized in that: A balance frame (15) is fixedly connected to both left and right ends of the outer wall of the liquid storage tank (8), and a sleeve ring (16) sleeved on the corresponding suspension rod (13) is fixedly connected to the end of the balance frame (15), and the screw ring (14) is supported on the lower side of the sleeve ring (16).

6. The device for rapid separation of soil Bacillus according to claim 1, characterized in that: A back plate (17) with a hollow structure is fixedly mounted on the front end of the back frame (2). The back plate (17) is tilted forward and downward. An auxiliary lamp (18) powered by an external mains power supply is mounted on the front end of the back plate (17).

7. A device for rapid separation of soil Bacillus according to claim 6, characterized in that: A blower (19) powered by an external mains electricity supply is installed at the rear end of the back plate (17), and an air outlet (20) communicating with an air outlet channel of the blower (19) is provided at the lower front end of the back plate (17).

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