Ultrasonic cell crusher

Abstract

The utility model discloses an ultrasonic cell crusher which comprises a main machine and a box body, an ultrasonic generator and a bearing platform are respectively arranged at the top and the bottom in the box body, the bearing platform comprises a water bath disc, a lifting mechanism and a rotating mechanism, the water bath disc is erected on the lifting mechanism, an overflow port is formed in the middle of the water bath disc, and a water storage disc is arranged below the overflow port. The rotating mechanism comprises a rotating platform, a crank, a telescopic shaft and a driving structure, the crank is located at the overflow port, the telescopic shaft is located at the middle axis of the overflow port, the rotating platform is arranged on the crank, the driving structure is arranged at the bottom of the box body, one end of the crank is connected with the telescopic shaft, and the telescopic shaft penetrates through the water storage disc to be connected with the driving structure; according to the ultrasonic crushing device, the temperature of a sample solution is controlled in a water bath mode, the water storage disc is used for storing liquid flowing down from the overflow port, and the rotating mechanism can enable the container filled with the sample solution to rotate continuously, so that the ultrasonic generator can stir the sample solution during working, and the crushing effect is improved.

Description

Technical Field

[0001] The utility model relates to the technical field of cell crushing, in particular to an ultrasonic cell crusher. Background Art

[0002] Ultrasonic cell disruptors are widely used in the biomedical field. The working principle of ultrasonic cell disruptors is to use the cavitation and mechanical action of high-intensity ultrasound to produce strong impact and shear force on cell membranes and cell walls, causing them to rupture and release cell contents, which is beneficial to the extraction and purification of intracellular proteins, DNA, RNA and other biological molecules.

[0003] Temperature is an important factor in the process of cell disruption. Low temperature can protect the stability of intracellular substances and reduce irreversible processes such as denaturation, coagulation, and inactivation of intracellular substances. Therefore, in the process of cell disruption, the ambient temperature needs to be controlled between 0-4°C.

[0004] However, the existing ultrasonic cell disruptors lack the ability to control the temperature of the sample solution when working, and the container containing the sample solution can usually only be placed in a fixed position. The sample solution lacks fluidity during the cell disruption process, which affects the overall cell disruption effect. Summary of the invention

[0005] The utility model aims to provide an ultrasonic cell disruptor to solve the problems existing in the prior art.

[0006] In order to achieve the above purpose, the technical solution adopted by the utility model is:

[0007] An ultrasonic cell disruptor comprises a main unit and a housing, wherein an ultrasonic generator and a supporting platform are respectively arranged at the top and the bottom of the housing, wherein the supporting platform comprises a water bath, a lifting mechanism and a rotating mechanism, wherein the water bath is mounted on the lifting mechanism, an overflow port is provided in the middle of the water bath, a water storage pan is provided below the overflow port, the rotating mechanism comprises a rotating platform, a crank, a telescopic shaft and a driving structure, wherein the crank is located at the overflow port, the telescopic shaft is located at the central axis of the overflow port, the rotating platform is arranged on the crank, the driving structure is arranged at the bottom of the housing, one end of the crank is connected to the telescopic shaft, and the telescopic shaft passes through the water storage pan and is connected to the driving structure.

[0008] The utility model adopts a water bath to control the temperature of the sample solution. The overflow port is arranged in the water bath tray to facilitate the installation and use of the rotating mechanism. The water storage tray is used to buffer the liquid flowing down from the overflow port. The rotating mechanism can make the container containing the sample solution rotate continuously, so that the ultrasonic generator can stir the sample solution when working, so that the sample solution can flow evenly and the crushing effect is improved.

[0009] Preferably, the lifting mechanism comprises a hydraulic push rod and a telescopic rod, the hydraulic push rod and the telescopic rod are both arranged at the bottom of the box body, and the hydraulic push rod and the telescopic rod are arranged around the central axis of the overflow port.

[0010] The lifting mechanism is used to adjust the height of the water bath, which, on the one hand, facilitates the ultrasonic generator to adapt to containers of different sizes, and on the other hand, can lower the height of the water bath to take out the container.

[0011] Preferably, a slide groove is provided at the bottom of the box body, and the slide groove is located between the hydraulic push rod and the telescopic rod. A water tank is provided under the water bath tray, and the water tank is slidably connected to the slide groove. The water tank is concave, and the water tank is partially arranged around the telescopic shaft and the driving structure.

[0012] The water tank moves along the chute to the outside of the box body, which is convenient for adding ice-water mixture into the water tank. At the same time, in order to expand the volume of the water tank and avoid the rotating mechanism, the water tank is designed to be concave.

[0013] Preferably, the water bath is provided with a first water inlet and a first water outlet, the top of the water tank is provided with a second water inlet and a second water outlet, a submersible pump is provided in the water tank, the submersible pump is connected to the second water outlet through a hose, the second water outlet is connected to the first water inlet through a hose, and the first water outlet is connected to the second water inlet through a hose.

[0014] Preferably, a third water outlet is provided on the water storage tray, a third water inlet is provided on the top of the water tank, and the third water outlet is connected to the third water inlet through a hose.

[0015] The utility model utilizes the water bath, the water storage tray and the water tank to form a water cycle, an ice-water mixture is added to the water tank, a submersible pump lifts low-temperature water into the water bath, the heated water flows back from the water bath to the water tank and the water storage tray, and the hot water in the water storage tray eventually flows back to the water tank, thereby ensuring the water bathing effect and preventing the water in the water bath from being heated in a short time and losing its cooling capacity.

[0016] Preferably, the water storage pan includes a bottom plate, a side plate and a cylindrical sleeve, the side plate is arranged around the central axis of the overflow port, the side plate is located between the water bath pan and the bottom plate, the cylindrical sleeve is arranged in the middle of the bottom plate, the two ends of the cylindrical sleeve are respectively protruded from the bottom plate, a rubber sealing ring is arranged in the cylindrical sleeve, and the telescopic shaft passes through the rubber sealing ring.

[0017] The water storage pan collects the water flowing from the overflow port and then sends it back to the water tank through the third water outlet. The cylindrical sleeve protrudes from the bottom plate to avoid the rubber sealing ring from bearing a large water pressure. The rubber sealing ring is used to block the gap between the cylindrical sleeve and the telescopic shaft to prevent water from directly overflowing to the bottom of the box.

[0018] Compared with the prior art, the beneficial effects of the utility model are:

[0019] (1) The utility model uses a water bath to control the temperature of the sample solution. An overflow port is provided in the water bath tray to facilitate the installation and use of the rotating mechanism. The water storage tray is used to buffer the liquid flowing down from the overflow port. The rotating mechanism can continuously rotate the container containing the sample solution, thereby enabling the ultrasonic generator to stir the sample solution during operation, so that the sample solution can flow evenly and improve the crushing effect.

[0020] (2) The utility model utilizes a water bath, a water storage pan and a water tank to form a water cycle. An ice-water mixture is added to the water tank. A submersible pump lifts low-temperature water into the water bath. The heated water flows back from the water bath to the water tank and the water storage pan. The hot water in the water storage pan eventually flows back to the water tank, thereby ensuring the water bathing effect and preventing the water in the water bath from being heated in a short period of time and losing its cooling ability. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] Figure 1 It is a schematic diagram of the structure of the utility model;

[0022] Figure 2 This is a schematic diagram of the structure of the box after the door is opened;

[0023] Figure 3 The structure after the box is cut open Figure 1 ;

[0024] Figure 4 The structure after the box is cut open Figure 2 ;

[0025] Figure 5 The structure after the box is cut open Figure 3 ;

[0026] Figure 6 It is a schematic diagram of the structure when the water bath tray is turned upside down;

[0027] Figure 7 It is a schematic diagram of the structure of the water storage pan;

[0028] In the figure: 1. box body; 2. ultrasonic generator; 3. water bath; 301. overflow; 302. first water inlet; 303. first water outlet; 4. hydraulic push rod; 5. telescopic rod; 6. rotating platform; 7. crank; 8. telescopic shaft; 9. driving structure; 10. water storage tray; 1001. third water outlet; 11. cylindrical sleeve; 12. water tank; 1201. second water inlet; 1202. second water outlet; 1203. third water inlet. DETAILED DESCRIPTION

[0029] The following will be combined with the drawings in the utility model to clearly and completely describe the technical solution of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.

[0030] In the description of the present invention, it should be noted that the directions or positional relationships indicated by terms such as "middle", "upper", "lower", "left", "right", "inside" and "outside" are based on the directions or positional relationships shown in the accompanying drawings, and are only for the convenience of describing the present invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific direction, be constructed and operated in a specific direction, and therefore cannot be understood as a limitation on the present invention.

[0031] like Figures 1 to 7 As shown, the specific scheme of the embodiment is as follows: an ultrasonic cell disruptor includes a main unit and a housing 1, wherein the top and bottom of the housing 1 are respectively provided with an ultrasonic generator 2 and a supporting platform, and the supporting platform includes a water bath 3, a lifting mechanism and a rotating mechanism.

[0032] The lifting mechanism includes a hydraulic push rod 4 and three telescopic rods 5, which are both arranged at the bottom of the box body 1, and the water bath 3 is mounted on the hydraulic push rod 4 and the telescopic rod 5. An overflow port 301 is opened in the middle of the water bath 3, and the hydraulic push rod 4 and the telescopic rod 5 are distributed at the four corners of the water bath 3. The hydraulic push rod 4 and the telescopic rod 5 are arranged around the central axis of the overflow port 301.

[0033] A water storage pan 10 is provided below the overflow port 301. The water storage pan 10 includes a bottom plate, a side plate and a cylindrical sleeve 11. The side plate is arranged around the central axis of the overflow port 301. The side plate is located between the water bath pan 3 and the bottom plate. The cylindrical sleeve 11 is arranged in the middle of the bottom plate. Both ends of the cylindrical sleeve 11 protrude out of the bottom plate respectively. A rubber sealing ring is arranged inside the cylindrical sleeve 11.

[0034] The rotating mechanism includes a rotating platform 6, a crank 7, a telescopic shaft 8 and a driving structure 9. The crank 7 is located at the overflow port 301, the telescopic shaft 8 is located at the central axis of the overflow port 301, the rotating platform 6 is arranged on the crank 7, and the rotating platform 6 is used to place a container containing a sample solution. The driving structure 9 is arranged at the bottom of the box body 1. The driving structure 9 adopts a motor and a gear reducer. One end of the crank 7 is connected to the telescopic shaft 8, and the telescopic shaft 8 is connected to the gear reducer through a rubber sealing ring, and the motor is connected to the telescopic shaft 8 through the gear reducer.

[0035] A slide groove is provided at the bottom of the box body 1, and the slide groove is located between the hydraulic push rod 4 and the telescopic rod 5. A water tank 12 is provided under the water bath plate 3, and the water tank 12 is slidably connected to the slide groove. The water tank 12 is concave, and the water tank 12 is partially arranged around the telescopic shaft 8 and the driving structure 9.

[0036] A first water inlet 302 and a first water outlet 303 are provided on the water bath tray 3, a second water inlet 1201 and a second water outlet 1202 are provided on the top of the water tank 12, a submersible pump is provided in the water tank 12, the submersible pump is connected to the second water outlet 1202 through a hose, the second water outlet 1202 is connected to the first water inlet 302 through a hose, and the first water outlet 303 is connected to the second water inlet 1201 through a hose.

[0037] A third water outlet 1001 is provided on the water storage pan 10, and a third water inlet 1203 is provided on the top of the water tank 12. The third water outlet 1001 is connected to the third water inlet 1203 through a hose.

[0038] When it is necessary to add water to the water tank 12, the water tank 12 is pulled out of the housing 1 along the chute portion, the cover of the water tank 12 is opened, and the ice-water mixture is poured into the water tank 12, and then the cover is closed and the water tank 12 is pushed back into the housing 1. During this process, since the hose has a sufficient length, the connectivity of the water inlets and outlets will not be affected.

[0039] When the lifting mechanism is working, the supporting platform works as follows: when the lifting mechanism lifts the water bath 3, the ultrasonic generator 2 will extend into the container containing the sample solution to start the cell disruption work, and the water bath 3 will drive the rotating platform 6 to rise. At this time, the telescopic shaft 8 can extend with the rotating platform 6; when the lifting mechanism lowers the water bath 3, the ultrasonic generator 2 will be separated from the container containing the sample solution to take out the container containing the sample solution, and the rotating platform 6 will press down the telescopic shaft 8 to shorten the telescopic shaft 8; when the lifting mechanism is working, the ultrasonic generator 2, the rotating mechanism and the submersible pump do not need to work.

[0040] When the ultrasonic generator 2 starts working, the working mode of the rotating mechanism is as follows: the motor drives the telescopic shaft 8 to rotate through the gear reducer, the telescopic shaft 8 drives the crank 7 to rotate, the crank 7 drives the rotating platform 6 to rotate, and the rotating platform 6 drives the container containing the sample solution to rotate, so that the ultrasonic generator 2 stirs the sample solution.

[0041] When the ultrasonic generator 2 starts working, the water circulation process of the device is as follows: the submersible pump pumps water into the water bath 3 through the second water outlet 1202 and the first water inlet 302. On the one hand, the water in the water bath 3 directly flows back to the water tank 12 through the first water outlet 303 and the second water inlet 1201, and on the other hand, it flows into the water storage tray 10 from the overflow port 301. The water in the water storage tray 10 flows back to the water tank 12 through the third water outlet 1001 and the third water inlet 1203. Since the cylindrical sleeve 11 protrudes from the bottom plate, only when too much water accumulates in the water storage tray 10 will it overflow the cylindrical sleeve 11, thereby enhancing the waterproofness of the cylindrical sleeve 11. The rubber sealing ring is used to fill the gap between the cylindrical sleeve 11 and the telescopic shaft 8, which can avoid direct friction between the two and prevent water from flowing out of the gap.

[0042] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. An ultrasonic cell disruptor, comprising a main unit and a housing, wherein an ultrasonic generator and a supporting platform are respectively arranged at the top and the bottom of the housing, It is characterized in that The supporting platform includes a water bath tray, a lifting mechanism and a rotating mechanism. The water bath tray is mounted on the lifting mechanism. An overflow port is opened in the middle of the water bath tray, and a water storage tray is arranged below the overflow port. The rotating mechanism includes a rotating platform, a crank, a telescopic shaft and a driving structure. The crank is located at the overflow port, and the telescopic shaft is located at the central axis of the overflow port. The rotating platform is arranged on the crank, and the driving structure is arranged at the bottom of the box. One end of the crank is connected to the telescopic shaft, and the telescopic shaft passes through the water storage tray and is connected to the driving structure.

2. An ultrasonic cell disruptor according to claim 1, It is characterized in that The lifting mechanism comprises a hydraulic push rod and a telescopic rod, both of which are arranged at the bottom of the box body, and the hydraulic push rod and the telescopic rod are arranged around the central axis of the overflow port.

3. An ultrasonic cell disruptor according to claim 2, It is characterized in that A slide groove is provided at the bottom of the box body, and the slide groove is located between the hydraulic push rod and the telescopic rod. A water tank is provided under the water bath tray, and the water tank is slidably connected to the slide groove. The water tank is concave, and the water tank part is arranged around the telescopic shaft and the driving structure.

4. An ultrasonic cell disruptor according to claim 3, It is characterized in that The water bath tray is provided with a first water inlet and a first water outlet, the top of the water tank is provided with a second water inlet and a second water outlet, a submersible pump is provided in the water tank, the submersible pump is connected to the second water outlet through a hose, the second water outlet is connected to the first water inlet through a hose, and the first water outlet is connected to the second water inlet through a hose.

5. An ultrasonic cell disruptor according to claim 3, It is characterized in that The water storage tray is provided with a third water outlet, the top of the water tank is provided with a third water inlet, and the third water outlet is communicated with the third water inlet through a hose.

6. An ultrasonic cell disruptor according to claim 1, It is characterized in that The water storage pan includes a bottom plate, a side plate and a cylindrical sleeve. The side plate is arranged around the central axis of the overflow port, and the side plate is located between the water bath pan and the bottom plate. The cylindrical sleeve is arranged in the middle of the bottom plate, and the two ends of the cylindrical sleeve protrude from the bottom plate respectively. A rubber sealing ring is arranged in the cylindrical sleeve, and the telescopic shaft passes through the rubber sealing ring.

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