Protein desalting device

Abstract

The utility model relates to the technical field of protein desalination, in particular to a protein desalination device which comprises a first pipe sleeve, a desalination mechanism and a fixing mechanism, and the fixing mechanism comprises a base, a vertical rod, a connecting assembly, a placing ring and a clamping assembly. According to the desalting device, the placing ring is arranged on the base, so that the longitudinal height of the placing ring is adjusted, then the first pipe sleeve is placed on the placing ring, the desalting mechanism is convenient to operate and observe by adjusting the height of the placing ring, and then the clamping assembly clamps the first pipe sleeve on the placing ring and fixes the first pipe sleeve, so that the stability of the first pipe sleeve on the placing ring is improved; finally, the protein solution can be injected into the desalting mechanism for a desalting experiment, the first pipe sleeve does not need to be held by hand in the whole process, the convenience of the experiment process is improved, and the problems that the first pipe sleeve is not provided with related fixing equipment, so that the experiment needs to be carried out by hand, and the convenience of the experiment is reduced are solved.

Description

Technical Field

[0001] The utility model relates to the technical field of protein desalination, in particular to a protein desalination device. Background Art

[0002] The laboratory often uses a desalting centrifugal column, which is used to add the protein solution to the desalting centrifugal column and perform centrifugal screening on a centrifuge. Since this desalting method must be used in conjunction with a centrifuge, the operation is cumbersome and costly.

[0003] At present, the prior art (CN209166965U) discloses a protein desalting device, including a first sleeve, a second sleeve, an enrichment component and a blowing component, wherein the second sleeve is non-sealed and arranged in the first sleeve, a waste liquid storage space is arranged between the bottom of the second sleeve and the bottom of the first sleeve, and a first through hole is arranged at the bottom of the second sleeve; the enrichment component is arranged in the second sleeve for protein enrichment; the blowing component is sealed and connected to the upper end of the second sleeve for blowing air into the second sleeve. The protein desalting device provided by the utility model blows air into the second sleeve through the blowing component to increase the internal air pressure of the second sleeve, and the protein solution flows into the waste liquid storage space through the enrichment component and the first through hole under the action of the air pressure, and the protein and polypeptide are collected in the enrichment component to achieve protein purification, thereby avoiding the disadvantages of the original technology of using a centrifuge, and having the advantages of simple operation and high cost performance.

[0004] In the above manner, the first pipe sleeve is not provided with relevant fixing equipment, so it needs to be handheld for experiment, which reduces the convenience of the experiment. Utility Model Content

[0005] The utility model aims to provide a protein desalting device, aiming to solve the problem that the first pipe sleeve is not provided with relevant fixing equipment, so the experiment needs to be carried out by hand, which reduces the convenience of the experiment.

[0006] To achieve the above-mentioned purpose, the utility model provides a protein desalting device, including a first pipe sleeve, a desalting mechanism and a fixing mechanism, the fixing mechanism including a base, a vertical rod, a connecting assembly, a placement ring and a clamping assembly, the desalting mechanism is arranged in the first pipe sleeve, the vertical rod is fixedly connected to the base and is located on the top of the base, the connecting assembly is slidably arranged on the outer side wall of the vertical rod, the placement ring is fixedly arranged on the outer side wall of the connecting assembly, the clamping assembly is arranged on the inner side wall of the placement ring, and the first pipe sleeve is located in the placement ring and contacts with the clamping assembly.

[0007] Wherein, the connecting assembly includes a slip ring, a supporting block and a screw rod. The slip ring is slidably connected to the vertical rod and is located on the outer wall of the vertical rod. The supporting block is slidably connected to the slip ring and is located on the inner wall of the slip ring. The screw rod is rotatably connected to the supporting block, is threadedly connected to the slip ring, and passes through the slip ring.

[0008] Wherein, the connecting assembly also includes a handle and a friction pad, the handle is fixedly connected to the screw rod and is located on a side away from the supporting block, and the friction pad is fixedly connected to the handle and is located on an outer side wall of the handle.

[0009] In which, the clamping assembly includes an arc plate, a return spring and a sliding rod, the return spring is fixedly connected to the placement ring and is located inside the placement ring, the sliding rod is fixedly connected to the return spring, slidably connected to the placement ring, and is located on the inner wall of the placement ring, the arc plate is fixedly connected to the sliding rod, contacts the outer wall of the first tube sleeve, and is located on the side away from the return spring.

[0010] Wherein, the fixing mechanism further includes a limit block and a buffer pad, the limit block is fixedly connected to the vertical rod and is located on a side away from the base, and the buffer pad is fixedly connected to the limit block and is located at the bottom of the limit block.

[0011] The utility model provides a protein desalting device. When conducting a protein desalting experiment, firstly, the connecting component is slid on the vertical rod to adjust the longitudinal height of the placement ring, and then the first tube sleeve is placed on the placement ring. By adjusting the height of the placement ring, it is convenient to operate and observe the desalting mechanism. Then, the clamping component clamps the first tube sleeve on the placement ring to fix the first tube sleeve to improve the stability of the first tube sleeve on the placement ring. Finally, the protein solution can be injected into the desalting mechanism to conduct a desalting experiment. The first tube sleeve does not need to be held by hand during the whole process, which improves the convenience of the experimental process and solves the problem that the first tube sleeve has no relevant fixing equipment, so it needs to be held by hand for the experiment, which reduces the convenience of the experiment. BRIEF DESCRIPTION OF THE DRAWINGS

[0012] In order to more clearly illustrate the embodiments of the present application or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art are briefly introduced below.

[0013] Figure 1 It is a structural schematic diagram of a protein desalting device according to the first embodiment of the utility model.

[0014] Figure 2 It is a cross-sectional view of a first pipe sleeve and a fixing mechanism of a protein desalting device according to the second embodiment of the utility model.

[0015] 101-first sleeve, 102-desalting mechanism, 103-fixing mechanism, 104-base, 105-vertical rod, 106-connecting assembly, 107-placing ring, 108-clamping assembly, 109-slip ring, 110-holding block, 111-screw, 112-handle, 113-friction pad, 114-arc plate, 115-reset spring, 116-slide rod, 201-limiting block, 202-buffer pad. DETAILED DESCRIPTION

[0016] The first embodiment of the present application is:

[0017] See also Figure 1 , Figure 1 It is a structural schematic diagram of a protein desalting device according to the first embodiment of the utility model.

[0018] The utility model provides a protein desalting device, including a first pipe sleeve 101, a desalting mechanism 102 and a fixing mechanism 103, wherein the fixing mechanism 103 includes a base 104, a vertical rod 105, a connecting assembly 106, a placement ring 107 and a clamping assembly 108, wherein the connecting assembly 106 includes a slip ring 109, a support block 110, a screw rod 111, a handle 112 and a friction pad 113, and the clamping assembly 108 includes an arc plate 114, a reset spring 115 and a slide rod 116. The above solution solves the problem that the first pipe sleeve 101 is not provided with relevant fixing equipment, so the experiment needs to be carried out by hand, which reduces the convenience of the experiment. It can be understood that the above solution can be used in the scene of protein desalting experiment.

[0019] According to the present specific embodiment, the protein desalting device comprises a first sleeve 101, a desalting mechanism 102 and a fixing mechanism 103, wherein the fixing mechanism 103 comprises a base 104, a vertical rod 105, a connecting assembly 106, a placement ring 107 and a clamping assembly 108, wherein the desalting mechanism 102 is arranged in the first sleeve 101, the vertical rod 105 is fixedly connected to the base 104 and is located at the top of the base 104, the connecting assembly 106 is slidably arranged on the outer wall of the vertical rod 105, the placement ring 107 is fixedly arranged on the outer wall of the connecting assembly 106, the clamping assembly 108 is arranged on the inner wall of the placement ring 107, and the first sleeve 101 is located in the placement ring 107 and is in contact with the clamping assembly 108. When conducting a protein desalting experiment, first, the connecting component 106 is slid on the vertical rod 105 to adjust the longitudinal height of the placement ring 107, and then the first tube sleeve 101 is placed on the placement ring 107. By adjusting the height of the placement ring 107, it is convenient to operate and observe the desalting mechanism 102. Then, the clamping component 108 clamps the first tube sleeve 101 on the placement ring 107 to fix the first tube sleeve 101 and improve the stability of the first tube sleeve 101 on the placement ring 107. Finally, the protein solution can be injected into the desalting mechanism 102 to conduct a desalting experiment. The whole process does not require holding the first tube sleeve 101, which improves the convenience of the experimental process and solves the problem that the first tube sleeve 101 is not provided with relevant fixing equipment, so it needs to be held by hand for the experiment, which reduces the convenience of the experiment.

[0020] The desalting mechanism 102 is described in the prior art as "a second sleeve, an enrichment component and a blowing component, the second sleeve is non-sealed and arranged in the first sleeve, a waste liquid storage space is arranged between the bottom of the second sleeve and the bottom of the first sleeve, and a first through hole is arranged at the bottom of the second sleeve; the enrichment component is arranged in the second sleeve for protein enrichment; the blowing component is sealed and connected to the upper end of the second sleeve for blowing air into the second sleeve. The protein desalting device provided by the utility model blows air into the second sleeve through the blowing component to increase the internal air pressure of the second sleeve, and the protein solution flows into the waste liquid storage space through the enrichment component and the first through hole under the action of the air pressure, and the protein and polypeptide are collected in the enrichment component to realize protein purification, thereby avoiding the disadvantages of the original technology of relying on a centrifuge, and has the advantages of simple operation and high cost performance".

[0021] The connection assembly 106 includes a slip ring 109, a support block 110 and a screw rod 111. The slip ring 109 is slidably connected to the vertical rod 105 and is located on the outer side wall of the vertical rod 105. The support block 110 is slidably connected to the slip ring 109 and is located on the inner side wall of the slip ring 109. The screw rod 111 is rotationally connected to the support block 110 and is threadedly connected to the slip ring 109 and penetrates the slip ring 109. The placement ring 107 is driven to slide on the vertical rod 105 by the slip ring 109, so that the height of the placement ring 107 can be adjusted. After the adjustment is completed, the screw rod 111 is screwed so that the screw rod 111 moves to the side close to the vertical rod 105 through threaded engagement, thereby pushing the support block 110 to support the vertical rod 105, so as to fix the position of the slip ring 109.

[0022] Secondly, the connecting assembly 106 further includes a handle 112 and a friction pad 113. The handle 112 is fixedly connected to the screw rod 111 and is located on a side away from the abutting block 110. The friction pad 113 is fixedly connected to the handle 112 and is located on an outer side wall of the handle 112. The handle 112 is used to increase the contact area between the screw rod 111 and the hand, and the friction pad 113 is used to increase the friction between the handle 112 and the hand, thereby improving the force effect on the screw rod 111.

[0023] At the same time, the clamping assembly 108 includes an arc plate 114, a reset spring 115 and a sliding rod 116. The reset spring 115 is fixedly connected to the placement ring 107 and is located inside the placement ring 107. The sliding rod 116 is fixedly connected to the reset spring 115 and is slidably connected to the placement ring 107 and is located on the inner wall of the placement ring 107. The arc plate 114 is fixedly connected to the sliding rod 116 and is in contact with the outer wall of the first sleeve 101 and is located on the side away from the reset spring 115. During the process of placing the first tube sleeve 101 into the placement ring 107, the first tube sleeve 101 pushes the slide rod 116 to squeeze the reset spring 115 through the arc plate 114 until the first tube sleeve 101 is placed. When the first tube sleeve 101 is placed in the placement ring 107 and there is a gap, the reset spring 115 resets and pushes the slide rod 116 to slide out of the placement ring 107. The slide rod 116 pushes the arc plate 114 to contact the first tube sleeve 101 to fill the gap, thereby improving the stability of the first tube sleeve 101 on the placement ring 107.

[0024] When conducting a protein desalting experiment, the placement ring 107 is first driven to slide on the vertical rod 105 by the slip ring 109 to adjust the height of the placement ring 107. After the adjustment is completed, the screw rod 111 is screwed to move toward the side close to the vertical rod 105 through threaded engagement, thereby pushing the abutment block 110 to abut the vertical rod 105 to fix the position of the slip ring 109. Then, the first pipe sleeve 101 is placed on the placement ring 107. By adjusting the height of the placement ring 107, it is convenient to operate and observe the desalting mechanism 102. Then, in the process of placing the first pipe sleeve 101 into the placement ring 107, the first pipe sleeve 101 is pushed by the arc plate 114. The slide bar 116 is moved to squeeze the reset spring 115 until the first tube sleeve 101 is placed. When the first tube sleeve 101 is placed in the placement ring 107 and there is a gap, the reset spring 115 is reset to push the slide bar 116 to slide out of the placement ring 107. The slide bar 116 pushes the arc plate 114 to contact the first tube sleeve 101 to fill the gap, thereby improving the stability of the first tube sleeve 101 on the placement ring 107. Finally, the protein solution can be injected into the desalination mechanism 102 for desalination experiment. The whole process does not need to hold the first tube sleeve 101, which improves the convenience of the experimental process and solves the problem that the first tube sleeve 101 is not provided with relevant fixing equipment, so it needs to be held for experiment, which reduces the convenience of the experiment.

[0025] The second embodiment of the present application is:

[0026] Based on the first embodiment, please refer to Figure 2 , Figure 2 It is a cross-sectional view of a first pipe sleeve and a fixing mechanism of a protein desalting device according to the second embodiment of the utility model.

[0027] The fixing mechanism 103 of the protein desalination device of this embodiment further includes a limit block 201 and a buffer pad 202 .

[0028] According to this specific embodiment, the fixing mechanism 103 also includes a limit block 201 and a buffer pad 202. The limit block 201 is fixedly connected to the vertical rod 105 and is located on a side away from the base 104. The buffer pad 202 is fixedly connected to the limit block 201 and is located at the bottom of the limit block 201.

[0029] The limit block 201 can prevent the slip ring 109 of the connection assembly 106 from sliding off the vertical rod 105 and then detaching, and the buffer pad 202 can prevent the slip ring 109 from colliding with the limit block 201 when the slip ring 109 slides and causes damage to the surfaces of both.

[0030] What is disclosed above is only one or more preferred embodiments of the present application, and cannot be used to limit the scope of rights of the present application. Ordinary technicians in this field can understand that all or part of the processes of implementing the above embodiments and equivalent changes made according to the claims of the present application are still within the scope covered by the present application.

Claims

1. A protein desalting device, comprising a first pipe sleeve and a desalting mechanism, wherein the desalting mechanism is arranged in the first pipe sleeve, characterized in that: Also included is a fixing mechanism, which includes a base, a vertical rod, a connecting assembly, a placement ring and a clamping assembly; The vertical rod is fixedly connected to the base and is located on the top of the base. The connecting component is slidably arranged on the outer wall of the vertical rod. The placement ring is fixedly arranged on the outer wall of the connecting component. The clamping component is arranged on the inner wall of the placement ring. The first pipe sleeve is located in the placement ring and contacts with the clamping component.

2. The protein desalination device according to claim 1, characterized in that The connecting assembly includes a slip ring, a supporting block and a screw rod. The slip ring is slidably connected to the vertical rod and is located on the outer wall of the vertical rod. The supporting block is slidably connected to the slip ring and is located on the inner wall of the slip ring. The screw rod is rotationally connected to the supporting block, is threadedly connected to the slip ring, and passes through the slip ring.

3. The protein desalination device according to claim 2, characterized in that: The connecting assembly also includes a handle and a friction pad. The handle is fixedly connected to the screw rod and is located on a side away from the abutting block. The friction pad is fixedly connected to the handle and is located on an outer side wall of the handle.

4. The protein desalination device according to claim 3, characterized in that: The clamping assembly includes an arc plate, a return spring and a sliding rod. The return spring is fixedly connected to the placement ring and is located inside the placement ring. The sliding rod is fixedly connected to the return spring and is slidably connected to the placement ring and is located on the inner wall of the placement ring. The arc plate is fixedly connected to the sliding rod and is in contact with the outer wall of the first tube sleeve and is located on a side away from the return spring.

5. The protein desalination device according to claim 1, characterized in that: The fixing mechanism further comprises a limit block and a buffer pad, wherein the limit block is fixedly connected to the vertical rod and is located at a side away from the base, and the buffer pad is fixedly connected to the limit block and is located at the bottom of the limit block.

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