Automatic heating and sealing device for fluorescent quantitative PCR (Polymerase Chain Reaction) test tube

Abstract

The utility model discloses a fluorescent quantitative PCR test tube automatic heating and sealing device which comprises a base, a storage roller, an air heater, a vertical plate, a placing frame and a cutting ring, two supports are fixedly welded to the left side of the top end of the base, the storage roller is movably placed at the top ends of the supports, and two fixing blocks are fixedly welded to the right end of the base; a recycling roller is movably installed between the side walls of the fixing blocks, two vertical plates are fixedly installed in the center of the top end of the base between the storage roller and the recycling roller, sliding grooves are formed in the side walls of the vertical plates, sliding strips are fixedly connected to the left side wall and the right side wall of the containing frame, and the sliding strips are transversely and slidably connected to the inner sides of the sliding grooves; a supporting frame is fixedly connected to the center of the edge of the top end of the base, an electric push rod is fixedly installed below the top end of the supporting frame, and a pressing plate is fixedly installed at the bottom of the electric push rod; the cutting ring is arranged to cut the heated sealing film, so that each test tube can be independently packaged and is convenient to take, and the practicability is relatively high.

Description

Technical Field

[0001] The present application relates to the technical field of test tube sealing equipment, and in particular to an automatic heating and sealing device for fluorescent quantitative PCR test tubes. Background Art

[0002] Fluorescence quantitative PCR is a technology that can monitor the PCR process in real time. By adding fluorescent groups to the PCR reaction system, the entire PCR process is monitored in real time using the accumulation of fluorescent signals, thereby achieving quantitative detection of target DNA fragments. Fluorescence quantitative PCR test tubes are usually made of highly transparent materials such as polypropylene or polycarbonate to facilitate observation of the progress of the PCR reaction. The test tubes need to be sealed during use to ensure that the samples will not be contaminated.

[0003] Application No. 201820865043.X discloses an automatic heating and sealing device for a fluorescent quantitative PCR test tube, including a fixed ring, a sealing film, a heating hole, a membrane disk and a sealing detector, etc. By setting the sealing film, the sealing material of the test tube can be made lighter and resource waste can be reduced. By setting the sealing detector, the sealing quality of the test tube can be tested to ensure the integrity of the test tube seal. By setting the fixed ring, the test tube can be made more stable when sealed, ensuring the sealing quality of the test tube.

[0004] However, in use, there are the following defects:

[0005] Although a whole piece of sealing film is used to seal the test tubes below through heating, the lack of a dividing device makes it impossible to take out the test tubes individually after sealing. Moreover, when taking out and gluing new sealing films, it cannot be guaranteed that only one sheet is adsorbed at a time, and multiple sheets may be glued together, which not only affects the subsequent heating effect, but also causes waste of sealing film. Utility Model Content

[0006] The purpose of the present application is to provide an automatic heating and sealing device for fluorescent quantitative PCR test tubes, which solves the problem proposed in the background technology that although a whole sealing film is used to seal the test tubes below by heating, due to the lack of a dividing device, the test tubes cannot be taken out individually after sealing, and when taking out and bonding a new sealing film, it cannot be guaranteed that only one sheet is adsorbed at a time, and multiple sheets may be bonded, which not only affects the subsequent heating effect, but also causes the problem of waste of sealing film.

[0007] To achieve the above-mentioned objectives, the present application provides the following technical solutions: an automatic heating and sealing device for fluorescent quantitative PCR test tubes, comprising a base, a storage roller, a hot air blower, a vertical plate, a placement rack and a cutting ring, wherein two brackets are fixedly welded to the left side of the top end of the base, a storage roller is movably placed on the top of the bracket, and two fixed blocks are fixedly welded to the right end of the base, a recovery roller is movably installed between the side walls of the fixed blocks, and two vertical plates are fixedly installed at the center of the top end of the base between the storage roller and the recovery roller, a sliding groove is provided on the side wall of the vertical plate, the left and right side walls of the placement rack are fixedly connected with sliding bars, and the sliding bars are laterally slidably connected on the inner side of the sliding groove, a number of test tube slots are evenly provided on the top of the placement rack, a support frame is fixedly connected to the center of the top edge of the base, an electric push rod is fixedly installed below the top of the support frame, and a pressure plate is fixedly installed at the bottom of the electric push rod.

[0008] In this technical solution, the test tube is inserted into the test tube slot, and then the slide bars on both sides of the placement rack are pushed into the slide groove so that the placement rack is located under the pressure plate, and then the sealing film on the storage roller is pulled out and connected to the recovery roller on the right. The electric push rod is powered on and started, driving the pressure plate to descend, pressing the test tube mouth, and the hot air blower is started after power is turned on to blow hot air from the heating hole at the bottom of the pressure plate, thereby heating the sealing film and making the sealing film adhere to the test tube mouth, thereby completing the sealing. The sealing film can be cut by the cutting ring, compression plate and shock absorber. During the process of pressing the pressure plate down, the sealing film has completed the sealing of the test tube after heating, and the top of the cutting ring will cut the sealing film. And when the cutting ring is subjected to the pressure of the pressing plate, it can be compressed downwards under the action of the compression plate and the shock absorber to prevent the pressing plate from being damaged by excessive downward pressure. The cut sealing film will be individually bonded to the opening of each test tube, so as to facilitate the individual and batch taking of the test tubes, which is more convenient and practical. At the same time, the sealing film can be automatically replaced and recycled through the driving motor and the recovery roller. After the sealing is completed, the driving motor is energized and started, driving the recovery roller to rotate, thereby rolling up the sealing film currently heated and sealed and cut, and pulling a new section of the sealing film to the bottom of the pressing plate, thereby eliminating the need for manual replacement of the sealing film, reducing the waste of the sealing film, and improving the sealing efficiency.

[0009] As an optional solution of the technical solution of the present application, hot air blowers are fixedly installed on the top of the pressure plates on both sides of the electric push rod, and a plurality of heating holes are evenly opened on the bottom of the pressure plate, and the heating holes are connected to the hot air blowers.

[0010] As an optional solution of the technical solution of the present application, a bottom groove is opened on the inner side of the placement rack below the test tube slot, and a plurality of shock absorbers are fixedly installed at the bottom of the bottom groove, and the top of the shock absorber is fixedly connected to the bottom of the compression plate.

[0011] As an optional solution of the technical solution of the present application, a cutting ring is vertically slidably connected to the surface of the placement rack outside the test tube slot, and the bottom of the cutting ring is fixedly connected to the top surface of the compression plate.

[0012] As an optional solution of the technical solution of the present application, a distribution box is fixedly installed on the side wall of the base on one side of the support frame.

[0013] As an optional solution of the technical solution of the present application, a driving motor is fixedly mounted on the side wall of the fixed block on the opposite side of one end of the recycling roller, and the rear end of the transmission shaft of the driving motor is fixedly connected to the recycling roller.

[0014] Compared with the prior art, the present invention has the following beneficial effects:

[0015] 1. The present application can cut the sealing film through the cutting ring, compression plate and shock absorber. During the downward pressing process of the pressure plate, the sealing film has completed sealing the test tube after heating, and the top of the cutting ring will cut the sealing film. The cutting ring can be compressed downward under the action of the compression plate and the shock absorber when subjected to the pressure of the pressure plate to prevent the pressure plate from being damaged by excessive downward pressure. The cut sealing film will be individually bonded to the opening of each test tube, thereby facilitating the individual and batch removal of the test tubes, which is more convenient and improves practicality.

[0016] 2. The present application can automatically replace and recycle the sealing film through the driving motor and the recovery roller. After the sealing is completed, the driving motor is energized and started, driving the recovery roller to rotate, thereby rolling up the sealing film that is currently heated, sealed and cut, and pulling the new section of the sealing film under the pressure plate, thereby eliminating the need for manual replacement of the sealing film, reducing the waste of the sealing film, and improving the sealing efficiency. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] Other features, objects and advantages of the present application will become more apparent by reading the detailed description of non-limiting embodiments with reference to the following drawings:

[0018] Figure 1 This is an overall view of an automatic heating and sealing device for fluorescent quantitative PCR test tubes in this application;

[0019] Figure 2 This is a schematic diagram of the bottom structure of a heating plate of an automatic heating and sealing device for fluorescent quantitative PCR test tubes in this application;

[0020] Figure 3 This is a front cross-sectional schematic diagram of an automatic heating and sealing device for a fluorescent quantitative PCR test tube according to the present application.

[0021] In the figure: 1. base; 2. bracket; 3. storage roller; 4. fixing block; 5. recovery roller; 6. driving motor; 7. support frame; 8. electric push rod; 9. pressing plate; 901. hot air blower; 10. heating hole; 11. distribution box; 12. vertical plate; 121. slide groove; 13. slide bar; 14. placement rack; 15. test tube slot; 16. cutting ring; 18. bottom groove; 19. shock absorber; 20. compression plate. DETAILED DESCRIPTION

[0022] In order to make the technical means, creative features, objectives and effects of the utility model easier to understand, they are further explained below in conjunction with specific implementation methods.

[0023] like Figure 1 As shown, an automatic heating and sealing device for fluorescent quantitative PCR test tubes includes a base 1, a storage roller 3, a hot air blower 901, a vertical plate 12, a placement rack 14 and a cutting ring 16. Two brackets 2 are fixedly welded to the left side of the top of the base 1, and the storage roller 3 is movably placed on the top of the bracket 2. Two fixed blocks 4 are fixedly welded to the right end of the base 1, and a recovery roller 5 is movably installed between the side walls of the fixed blocks 4. Two vertical plates 12 are fixedly installed in the center of the top of the base 1 between the storage roller 3 and the recovery roller 5.

[0024] like Figure 1 As shown, a driving motor 6 is fixedly mounted on the side wall of the fixing block 4 on the opposite side of one end of the recovery roller 5, and the rear end of the transmission shaft of the driving motor 6 is fixedly connected to the recovery roller 5, so that the recovery roller 5 can quickly replace and recover the sealing film.

[0025] like Figure 1 As shown, a support frame 7 is fixedly connected to the center of the top edge of the base 1, a distribution box 11 is fixedly installed on the side wall of the base 1 on one side of the support frame 7, an electric push rod 8 is fixedly installed below the top of the support frame 7, and a pressure plate 9 is fixedly installed at the bottom of the electric push rod 8. The pressure plate 9 can press the sealing film downward to make it contact the opening of the test tube.

[0026] like Figure 2 As shown, hot air blowers 901 are fixedly installed on the top of the pressing plates 9 on both sides of the electric push rod 8, and a plurality of heating holes 10 are evenly opened on the bottom of the pressing plate 9. The heating holes 10 are connected to the hot air blower 901. The hot air blower 901 can heat the sealing film to make it adhere to the mouth of the test tube, thereby completing the sealing.

[0027] like Figure 1 and Figure 3 As shown, a plurality of test tube slots 15 are evenly arranged at the top of the placement rack 14, a bottom groove 18 is arranged on the inner side of the placement rack 14 below the test tube slots 15, a plurality of shock absorbers 19 are fixedly installed at the bottom of the bottom groove 18, the top of the shock absorber 19 is fixedly connected to the bottom of the compression plate 20, and the shock absorber 19 can enable the compression plate 20 and the cutting ring 16 to move up and down.

[0028] like Figure 3 As shown, a slide groove 121 is formed on the side wall of the vertical plate 12 , and the left and right side walls of the placement rack 14 are fixedly connected with a slide bar 13 , and the slide bar 13 is laterally slidably connected inside the slide groove 121 .

[0029] like Figure 3 As shown, a cutting ring 16 is vertically slidably connected to the surface of the placement rack 14 outside the test tube slot 15, and the bottom of the cutting ring 16 is fixedly connected to the top surface of the compression plate 20. The top of the cutting ring 16 is relatively sharp and can cut the sealing film.

[0030] In actual use, the test tube is inserted into the test tube slot 15, and then the slide bars 13 on both sides of the placement rack 14 are pushed into the slide groove 121, so that the placement rack 14 is located below the pressure plate 9, and then the sealing film on the storage roller 3 is pulled out and connected to the recovery roller 5 on the right. The electric push rod 8 is powered on and started, driving the pressure plate 9 to descend, pressing the test tube mouth, and the hot air blower 901 is started after power is turned on to blow hot air from the heating hole 10 at the bottom of the pressure plate 9, thereby heating the sealing film and making the sealing film adhere to the test tube mouth, thereby completing the sealing. The sealing film can be cut by the cutting ring 16, the compression plate 20 and the shock absorber 19. In the process of pressing the pressure plate 9 downward, the sealing film has completed the sealing of the test tube after heating, and the top of the cutting ring 16 will The sealing film is cut, and the cutting ring 16 can be compressed downward under the action of the compression plate 20 and the shock absorber when subjected to the pressure of the pressure plate 9, so as to prevent the pressure from being too large and damaging the pressure plate 9. The cut sealing film will be individually bonded to the opening of each test tube, so as to facilitate the individual and batch taking of the test tubes, which is more convenient and practical. At the same time, the sealing film can be automatically replaced and recycled by driving the motor 6 and the recovery roller 5. After the sealing is completed, the driving motor 6 is powered on and started, driving the recovery roller 5 to rotate, so as to roll up the sealing film that is currently heated, sealed and cut, and pull the new section of the sealing film to the bottom of the pressure plate 9, thereby eliminating the need for manual replacement of the sealing film, reducing the waste of the sealing film, and improving the sealing efficiency.

Claims

1. A fluorescent quantitative PCR test tube automatic heating and sealing device, comprising a base (1), a storage roller (3), a hot air blower (901), a vertical plate (12), a placement rack (14) and a cutting ring (16), characterized in that: Two brackets (2) are fixedly welded on the left side of the top of the base (1), and a storage roller (3) is movably placed on the top of the bracket (2). Two fixed blocks (4) are fixedly welded on the right end of the base (1). A recovery roller (5) is movably installed between the side walls of the fixed blocks (4), and two vertical plates (12) are fixedly installed at the center of the top of the base (1) between the storage roller (3) and the recovery roller (5). The side walls of the vertical plates (12) are provided with sliding grooves (121). The left and right side walls of the placement rack (14) are fixedly connected with sliding bars (13), and the sliding bars (13) are laterally slidably connected on the inner side of the sliding grooves (121). A plurality of test tube slots (15) are evenly opened at the top of the placement rack (14). A support frame (7) is fixedly connected to the center of the top edge of the base (1). An electric push rod (8) is fixedly installed below the top of the support frame (7), and a pressing plate (9) is fixedly installed at the bottom of the electric push rod (8).

2. The automatic heating and sealing device for fluorescent quantitative PCR test tubes according to claim 1, characterized in that: A hot air blower (901) is fixedly mounted on the top of the pressing plates (9) on both sides of the electric push rod (8), and a plurality of heating holes (10) are evenly arranged on the bottom of the pressing plates (9), and the heating holes (10) are connected to the hot air blower (901).

3. The automatic heating and sealing device for fluorescent quantitative PCR test tubes according to claim 1, characterized in that: A bottom groove (18) is provided inside the placement rack (14) below the test tube slot (15), and a plurality of shock absorbers (19) are fixedly installed at the bottom of the bottom groove (18), and the top of the shock absorber (19) is fixedly connected to the bottom of the compression plate (20).

4. The automatic heating and sealing device for fluorescent quantitative PCR test tubes according to claim 3, characterized in that: A cutting ring (16) is vertically slidably connected to the surface of the placement rack (14) outside the test tube slot (15), and the bottom of the cutting ring (16) is fixedly connected to the top surface of the compression plate (20).

5. The automatic heating and sealing device for fluorescent quantitative PCR test tubes according to claim 1, characterized in that: A distribution box (11) is fixedly mounted on the side wall of the base (1) on one side of the support frame (7).

6. The automatic heating and sealing device for fluorescent quantitative PCR test tubes according to claim 1, characterized in that: A driving motor (6) is fixedly mounted on the side wall of the fixed block (4) on the opposite side of one end of the recycling roller (5), and the rear end of the transmission shaft of the driving motor (6) is fixedly connected to the recycling roller (5).

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