Peripheral blood mononuclear cell separation device

Abstract

The utility model discloses a peripheral blood mononuclear cell separation device which is characterized in that the inner surface of a separation device shell is rotatably connected with an outer cylinder, the lower surface of the separation device shell is fixedly connected with a motor, the output end of the motor is fixedly connected to the lower surface of the outer cylinder, the inner surface of the outer cylinder is movably connected with an inner cylinder, and a clamping groove is formed in the outer cylinder; the outer surface of the inner cylinder is fixedly connected with a clamping block, the clamping groove is in clearance fit with the clamping block, the upper surface of the inner cylinder is movably connected with a cover plate, the upper surface of the separation device shell is rotationally connected with a sealing cover, the upper surface of the sealing cover is fixedly connected with a hydraulic cylinder, the output end of the hydraulic cylinder is fixedly connected with a telescopic rod, and the other end of the telescopic rod is fixedly connected with a connecting rod. The side surface of the connecting rod is fixedly connected with bristles, the side surfaces of the bristles are movably connected with the inner surface of the inner barrel, through the components, the inner surface of the inner barrel can be automatically brushed, and the workload of operators can be reduced.

Description

Technical Field

[0001] The utility model relates to the technical field of separation devices, in particular to a peripheral blood mononuclear cell separation device. Background Art

[0002] The existing peripheral blood mononuclear cell separation device extracts mononuclear cells from the peripheral blood sample through a filter or a centrifugal cylinder after placing the peripheral blood sample inside the separation device. However, when it is used, it does not have a structure for automatically cleaning the inside of the machine. Since there is no structure for automatically cleaning the inside of the machine, manual cleaning requires multiple repeated operations to ensure that the inside of the machine is clean and hygienic, which consumes time and energy. For example, a Chinese patent discloses a "peripheral blood mononuclear cell rapid separation device" with an application number of "201922205534.9". This device allows the drug solution to directly enter the body through the drug solution tank and the liquid outlet pipe, and the peripheral blood mononuclear cell solution and the drug solution can be fully mixed by the stirring blades on the surface of the rotating column. The peripheral blood mononuclear cell solution can be fully separated by the first filter and the second filter, and the inside of the body can be fully heated by the electric heating network and the heat conducting plate to achieve a better separation effect. However, when it is used, its internal structure is relatively complex and does not have the function of automatic cleaning. Manual cleaning requires multiple repeated operations to ensure that the inside of the machine is clean and hygienic, which consumes too much time and energy. Utility Model Content

[0003] The utility model aims to solve at least one of the technical problems existing in the prior art, and provides a peripheral blood mononuclear cell separation device, which is lowered into the inner cylinder by a telescopic rod with a connecting rod and bristles, and the inner cylinder is rotated by a motor to drive the outer cylinder so that the bristles can automatically scrub the inner surface of the inner cylinder, which can reduce the workload of operators, and no longer need to manually clean and maintain the device, saving time and human resources, and avoiding cross contamination. Automatic cleaning can ensure the internal cleanliness of the device and avoid cross contamination between different samples, which is very important for laboratories that need to process multiple samples, and can ensure the accuracy and reliability of the separation results. By adding the peripheral blood sample into the inner cylinder, the outer cylinder is driven by a motor to rotate the inner cylinder to generate centrifugal force, and the mononuclear cells are separated, which can be extracted quickly and efficiently. The process of extracting peripheral blood mononuclear cells by centrifugal force is relatively fast and efficient, and the cell separation can be completed in a shorter time, thereby improving the efficiency of the experiment. When extracting peripheral blood mononuclear cells by centrifugal force, the use of chemical reagents or enzyme treatment can be avoided, so the integrity and biological activity of the cells can be better maintained.

[0004] The utility model also provides the above-mentioned peripheral blood mononuclear cell separation device, comprising: a separation device shell, the inner surface of the separation device shell is rotatably connected with an outer cylinder, the lower surface of the separation device shell is fixedly connected with a motor, the output end of the motor is fixedly connected to the lower surface of the outer cylinder, the inner surface of the outer cylinder is movably connected with the inner cylinder, a card slot is provided on the outer cylinder, the outer surface of the inner cylinder is fixedly connected with a card block, the card slot and the card block are clearance-matched, the upper surface of the inner cylinder is movably connected with a cover plate, the upper surface of the separation device shell is rotatably connected with a sealing cover, the upper surface of the sealing cover is fixedly connected with a hydraulic cylinder, the output end of the hydraulic cylinder is fixedly connected with a telescopic rod, the other end of the telescopic rod is fixedly connected with a connecting rod, the side surface of the connecting rod is fixedly connected with bristles, and the side surface of the bristles is movably connected to the inner surface of the inner cylinder. Through the above components, the inner surface of the inner cylinder can be automatically scrubbed, which can reduce the workload of operators.

[0005] According to the peripheral blood mononuclear cell separation device of the utility model, a handle is fixedly connected to the upper surface of the sealing cover, and an anti-slip sleeve is fixedly connected to the outer surface of the handle. The above components facilitate the use of the sealing cover.

[0006] According to the peripheral blood mononuclear cell separation device described in the utility model, a sealing gasket is fixedly connected to the lower surface of the sealing cover, and the side surface of the sealing gasket is movably connected to the inner surface of the separation device shell. The above components facilitate the improvement of the sealing performance of the sealing cover.

[0007] According to the peripheral blood mononuclear cell separation device of the utility model, a handle is fixedly connected to the side surface of the separation device shell, and the above components are convenient for users to move the device.

[0008] According to the peripheral blood mononuclear cell separation device of the utility model, a base is fixedly connected to the lower surface of the separation device shell, and the above components facilitate the bearing of the device.

[0009] According to the peripheral blood mononuclear cell separation device described in the utility model, the lower surface of the base is fixedly connected with a support leg, and the lower surface of the support leg is fixedly connected with an anti-slip pad, and the above components are used to facilitate lifting the equipment.

[0010] According to the peripheral blood mononuclear cell separation device described in the utility model, the inner surface of the separation device shell is fixedly connected to a heat conducting plate, the lower surface of the separation device shell is fixedly connected to a heater, and the output end of the heater is fixedly connected to the side surface of the heat conducting plate. Through the above components, it is convenient to keep a constant temperature inside the separation device shell and maintain the activity of single cells.

[0011] According to the peripheral blood mononuclear cell separation device described in the utility model, the side surface of the cover plate is fixedly connected with a damping pad, and the outer surface of the damping pad is movably connected to the inner surface of the inner tube. Through the above components, the cover plate can be installed more tightly and is not easy to fall off.

[0012] Beneficial Effects

[0013] 1. Compared with the prior art, the peripheral blood mononuclear cell separation device, through the telescopic rod, carries the connecting rod and the bristles to the inside of the inner cylinder, and drives the outer cylinder to rotate the inner cylinder through the motor, so that the bristles can automatically brush the inner surface of the inner cylinder, which can reduce the workload of the operator, and no longer need to manually clean and maintain the device, saving time and human resources, and avoiding cross contamination. Automatic cleaning can ensure the internal cleanliness of the device and avoid cross contamination between different samples. This is very important for laboratories that need to process multiple samples and can ensure the accuracy and reliability of the separation results.

[0014] 2. Compared with the prior art, the peripheral blood mononuclear cell separation device can extract mononuclear cells quickly and efficiently by adding the peripheral blood sample into the inner cylinder and driving the outer cylinder to rotate the inner cylinder to generate centrifugal force through a motor. The process of extracting peripheral blood mononuclear cells by centrifugal force is relatively fast and efficient, and the cell separation can be completed in a shorter time, thereby improving the efficiency of the experiment. When extracting peripheral blood mononuclear cells by centrifugal force, the use of chemical reagents or enzyme treatment can be avoided, thereby better maintaining the integrity and biological activity of the cells. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] The utility model is further described below in conjunction with the accompanying drawings and embodiments;

[0016] Figure 1 This is a main structural diagram of the peripheral blood mononuclear cell separation device of the utility model;

[0017] Figure 2 This is a cross-sectional structural diagram of the peripheral blood mononuclear cell separation device of the utility model;

[0018] Figure 3 This is a rear structural diagram of the peripheral blood mononuclear cell separation device of the utility model;

[0019] Figure 4 This is a left view of the structure of the peripheral blood mononuclear cell separation device of the utility model.

[0020] Legend:

[0021] 1. Sealing cover; 2. Handle; 3. Anti-slip cover; 4. Separation device housing; 5. Support leg; 6. Anti-slip pad; 7. Hydraulic cylinder; 8. Handle; 9. Base; 10. Telescopic rod; 11. Block; 12. Slot; 13. Cover plate; 14. Inner tube; 15. Outer tube; 16. Motor; 17. Sealing pad; 18. Connecting rod; 19. Bristles; 20. Damping pad; 21. Heat transfer plate; 22. Heater. DETAILED DESCRIPTION

[0022] This section will describe in detail the specific embodiments of the utility model. The preferred embodiments of the utility model are shown in the accompanying drawings. The purpose of the accompanying drawings is to supplement the description of the text part of the specification with graphics, so that people can intuitively and vividly understand each technical feature and the overall technical solution of the utility model, but it cannot be understood as a limitation on the protection scope of the utility model.

[0023] Reference Figure 1-4 The peripheral blood mononuclear cell separation device of the utility model embodiment comprises: a separation device shell 4, a handle 8 is fixedly connected to the side surface of the separation device shell 4 for the user to move the device, a base 9 is fixedly connected to the lower surface of the separation device shell 4 for carrying the device, a supporting leg 5 is fixedly connected to the lower surface of the base 9, and a non-slip pad 6 is fixedly connected to the lower surface of the supporting leg 5 for raising the device for easy use, a heat conducting plate 21 is fixedly connected to the inner surface of the separation device shell 4, a heater 22 is fixedly connected to the lower surface of the separation device shell 4, and the output end of the heater 22 is fixedly connected to the side surface of the heat conducting plate 21 for keeping the internal temperature of the device constant, an outer cylinder 15 is rotatably connected to the inner surface of the separation device shell 4 for driving the inner cylinder to rotate, a motor 16 is fixedly connected to the lower surface of the separation device shell 4, and the output end of the motor 16 is fixedly connected to the lower surface of the outer cylinder 15, and the inner surface of the outer cylinder 15 is movably connected to the inner cylinder 14 for separating mononuclear cells therein.

[0024] A card slot 12 is provided on the outer cylinder 15, and a card block 11 is fixedly connected to the outer surface of the inner cylinder 14. The card slot 12 and the card block 11 are clearance-matched, so that the outer cylinder can drive the inner cylinder to move. A cover plate 13 is movably connected to the upper surface of the inner cylinder 14 to prevent the peripheral blood sample from splashing out during rotation. A damping pad 20 is fixedly connected to the side surface of the cover plate 13. The outer surface of the damping pad 20 is movably connected to the inner surface of the inner cylinder 14 to increase the connection strength of the cover plate. A sealing cover 1 is rotatably connected to the upper surface of the separation device housing 4 for sealing the device. A handle 2 is fixedly connected to the upper surface of the sealing cover 1, and an anti-slip sleeve 3 is fixedly connected to the outer surface of the handle 2 for the user to use the sealing cover.

[0025] A sealing gasket 17 is fixedly connected to the lower surface of the sealing cover 1, and the side surface of the sealing gasket 17 is movably connected to the inner surface of the separation device housing 4 to increase the sealing performance of the sealing cover. A hydraulic cylinder 7 is fixedly connected to the upper surface of the sealing cover 1, and a telescopic rod 10 is fixedly connected to the output end of the hydraulic cylinder 7 to drive the connecting rod to rise and fall so that the bristles can brush the inner cylinder. A connecting rod 18 is fixedly connected to the other end of the telescopic rod 10, and bristles 19 are fixedly connected to the side surface of the connecting rod 18 to brush the inner cylinder. The side surface of the bristles 19 is movably connected to the inner surface of the inner cylinder 14.

[0026] Working principle: When in use, the separation device housing 4 is opened through the handle 2, the inner cylinder 14 is taken out, the cover 13 is opened, and the peripheral blood sample is added into the inner cylinder 14, and the cover 13 is put back, and the inner cylinder 14 is put back into the outer cylinder 15, and the card block 11 is inserted into the card slot 12 to ensure that it can be smoothly driven by the outer cylinder 15. After placement, the sealing cover 1 is put back, and the motor 16 is turned on to drive the outer cylinder 15 and the inner cylinder 14 to rotate, so that centrifugal force is generated inside to separate the mononuclear cells in the peripheral blood sample inside. After use, when cleaning the inside, add disinfectant cleaning liquid into the inner cylinder 14, and the connecting rod 18 with the brush 19 is lowered through the telescopic rod 10, and the outer cylinder 15 and the inner cylinder 14 are driven to rotate by the motor 16, so that the brush 19 brushes the inside.

[0027] The embodiments of the present invention are described in detail above in conjunction with the accompanying drawings, but the present invention is not limited to the above embodiments, and various changes can be made within the knowledge scope of ordinary technicians in the relevant technical field without departing from the purpose of the present invention.

Claims

1. A peripheral blood mononuclear cell separation device, characterized in that: include: A separation device housing (4), wherein the inner surface of the separation device housing (4) is rotatably connected to an outer cylinder (15), the lower surface of the separation device housing (4) is fixedly connected to a motor (16), the output end of the motor (16) is fixedly connected to the lower surface of the outer cylinder (15), the inner surface of the outer cylinder (15) is movably connected to an inner cylinder (14), a clamping groove (12) is provided on the outer cylinder (15), and a clamping block (11) is fixedly connected to the outer surface of the inner cylinder (14); The card slot (12) and the card block (11) are loosely matched, the upper surface of the inner cylinder (14) is movably connected to a cover plate (13), the upper surface of the separation device housing (4) is rotatably connected to a sealing cover (1), the upper surface of the sealing cover (1) is fixedly connected to a hydraulic cylinder (7), the output end of the hydraulic cylinder (7) is fixedly connected to a telescopic rod (10), the other end of the telescopic rod (10) is fixedly connected to a connecting rod (18), the side surface of the connecting rod (18) is fixedly connected to bristles (19), and the side surface of the bristles (19) is movably connected to the inner surface of the inner cylinder (14).

2. The peripheral blood mononuclear cell separation device according to claim 1, characterized in that: The upper surface of the sealing cover (1) is fixedly connected to a handle (2), and the outer surface of the handle (2) is fixedly connected to an anti-slip sleeve (3).

3. The peripheral blood mononuclear cell separation device according to claim 1, characterized in that: A sealing gasket (17) is fixedly connected to the lower surface of the sealing cover (1), and a side surface of the sealing gasket (17) is movably connected to the inner surface of the separation device housing (4).

4. The peripheral blood mononuclear cell separation device according to claim 1, characterized in that: A handle (8) is fixedly connected to the side surface of the separation device housing (4).

5. The peripheral blood mononuclear cell separation device according to claim 1, characterized in that: A base (9) is fixedly connected to the lower surface of the separation device housing (4).

6. The peripheral blood mononuclear cell separation device according to claim 5, characterized in that: The lower surface of the base (9) is fixedly connected to a support leg (5), and the lower surface of the support leg (5) is fixedly connected to an anti-slip pad (6).

7. The peripheral blood mononuclear cell separation device according to claim 1, characterized in that: A heat conducting plate (21) is fixedly connected to the inner surface of the separation device housing (4), a heater (22) is fixedly connected to the lower surface of the separation device housing (4), and an output end of the heater (22) is fixedly connected to the side surface of the heat conducting plate (21).

8. The peripheral blood mononuclear cell separation device according to claim 1, characterized in that: A damping pad (20) is fixedly connected to the side surface of the cover plate (13), and the outer surface of the damping pad (20) is movably connected to the inner surface of the inner tube (14).

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