Fluorescence detection equipment

Abstract

The utility model discloses fluorescence detection equipment, which relates to the technical field of fluorescence detection and comprises a shell, a plurality of detection holes are arranged on the shell, optical filters are arranged at the detection holes, a plurality of detection stations corresponding to the detection holes are arranged in the shell, and a PCR (polymerase chain reaction) tube and an LED (light-emitting diode) lamp are arranged on each detection station. Light emitted by the LED lamp can penetrate through the PCR tube and the optical filter and is projected to the light intensity detection module, a heating device capable of heating the PCR tube is arranged in the shell, and a digital temperature control meter in communication connection with the heating device is arranged in the shell. The fluorescence detection equipment provided by the utility model can improve the detection efficiency, and is convenient to carry and flexible to use.

Description

Technical Field

[0001] The utility model relates to the technical field of fluorescence detection, in particular to a fluorescence detection device. Background Art

[0002] PCR (polymerase chain reaction) is a technique used to amplify DNA fragments. Through repeated denaturation, primer binding and extension steps under temperature control, PCR can amplify DNA templates in a short period of time and produce a large amount of target DNA. PCR is widely used in gene sequencing, genetic research and disease diagnosis. It has high sensitivity and specificity and is an important tool in molecular biology.

[0003] Optical imaging visualization is a technology that uses fluorescent dyes to label samples and uses a camera module to display fluorescent images. The fluorescence spectrum is selectively filtered and separated by filters of specific wavelengths, and the camera module takes pictures. The images can be displayed and processed in real time through imaging devices such as computers or mobile phones. Fluorescence imaging visualization technology plays an important role in life science research. It can provide high signal-to-noise ratio and high-resolution fluorescent images to help researchers observe and analyze reaction processes and compare reaction results. Therefore, fluorescence imaging visualization research of PCR instruments is very necessary.

[0004] Traditional instruments are usually fixed on a workbench and cannot be moved, so they need to be carried manually. When conducting some outdoor experiments, due to the heavy weight of the PCR instrument itself, it is very inconvenient to carry it over long distances. It is not only labor-intensive but also poses a risk of falling. Utility Model Content

[0005] The purpose of the utility model is to provide a fluorescence detection device to solve the problems existing in the above-mentioned prior art, which can improve the detection efficiency, is easy to carry and flexible to use.

[0006] To achieve the above purpose, the utility model provides the following solutions:

[0007] The utility model provides a fluorescence detection device, comprising a shell, wherein a plurality of detection holes are opened on the shell, filters are arranged at the detection holes, a plurality of detection stations are arranged inside the shell corresponding to the detection holes, a PCR tube and an LED lamp are arranged on each of the detection stations, the light emitted by the LED lamp can pass through the PCR tube and the filter and be projected onto a light intensity detection module, a heating device capable of heating the PCR tube is arranged inside the shell, and a digital temperature control meter communicatively connected to the heating device is arranged inside the shell.

[0008] Preferably, a bracket is fixedly arranged inside the housing, and the filter is fixedly arranged on the bracket.

[0009] Preferably, the wavelength of the LED lamp is 450nm or 540nm.

[0010] Preferably, the heating device comprises a heat sink, a heating plate fixedly disposed on the heat sink, and a temperature sensor fixedly disposed on the heat sink, and both the temperature sensor and the heating plate are communicatively connected to the digital temperature control meter.

[0011] Preferably, the heating sheet and the temperature sensor are both adhered to the heat sink by thermally conductive silica gel.

[0012] Preferably, the heating sheet is made of ceramic material.

[0013] Preferably, the light intensity detection module is a light intensity meter.

[0014] Compared with the prior art, the utility model has achieved the following technical effects:

[0015] The fluorescence detection device provided by the utility model is provided with a plurality of detection stations, and can detect samples at the same time, which greatly improves the detection efficiency. At the same time, the PCR tube can be heated by the heating device so that the sample can produce amplification phenomenon. The light intensity detection module can detect the light intensity value passing through the PCR tube. The device is functionally integrated, easy to carry, flexible to use, and can meet the requirements of fluorescence detection in multiple scenarios. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] In order to more clearly illustrate the embodiments of the utility model or the technical solutions in the prior art, the drawings required for use in the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the utility model. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0017] Figure 1 A schematic diagram of the structure of the fluorescence detection device provided by the utility model;

[0018] Figure 2 A schematic diagram of the internal structure of the fluorescence detection device provided by the utility model;

[0019] Figure 3 A cross-sectional view of the internal structure of the fluorescence detection device provided by the utility model;

[0020] Figure 4 A schematic diagram of the structure of the heating device provided by the utility model;

[0021] In the figure: 1-housing; 11-filter; 12-bracket; 2-PCR tube; 3-LED lamp; 4-heat sink; 5-heating plate; 6-temperature sensor; 7-digital temperature controller. DETAILED DESCRIPTION

[0022] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.

[0023] The utility model aims to provide a fluorescence detection device to solve the problems existing in the prior art, improve the detection efficiency, and is easy to carry and flexible to use.

[0024] In order to make the above-mentioned purposes, features and advantages of the present invention more obvious and easy to understand, the present invention is further described in detail below in conjunction with the accompanying drawings and specific implementation methods.

[0025] The utility model provides a fluorescence detection device, which is designed by Solidworks software. Figures 1 to 4 As shown, in this embodiment, it includes a shell 1, which is made of an acrylic plate after laser cutting. The top outer surface of the shell 1 is sprayed black with ABS and silk-screened, and can pass full-light UV. The outer side is sprayed white with ABS, matte, and five-point elimination. The bottom outer surface is sprayed black with ABS, matte, and five-point elimination, and silk-screened. A plurality of detection holes are opened on the shell 1, and a filter 11 is arranged at the detection hole. A plurality of detection stations are arranged inside the shell 1 corresponding to each detection hole. Preferably, there are four detection stations, which can be used for four samples to be detected at the same time, Ex / Em=450 / 530nm and Ex / Em=541 / 590nm, two each. Each detection station is provided with a PCR tube 2 and an LED lamp 3. The light emitted by the LED lamp 3 can pass through the PCR tube 2 and the filter 11 and be projected onto the light intensity detection module. A heating device capable of heating the PCR tube 2 is provided inside the outer shell 1. A digital temperature controller 7 communicating with the heating device is provided inside the outer shell 1. A button capable of controlling the operation of the equipment is provided on the outer shell 1. A 5V rechargeable lithium battery is provided inside the outer shell 1 for powering the LED lamp 3, the heating device and the digital temperature controller 7. A corresponding charging port is provided on the outer shell 1.

[0026] A bracket 12 is fixedly arranged inside the housing 1, and the filter 11 is fixedly arranged on the bracket 12, and the bracket 12 is made by 3D printing.

[0027] The excitation wavelength of the LED lamp 3 is 450nm or 540nm, wherein the excitation wavelength of the LED lamp 3 at two detection stations is 450nm, and the excitation wavelength of the LED lamp 3 at the other two detection stations is 540nm. The filter 11 matching the LED lamp 3 adopts a narrow-band filter 11, thereby realizing two detection modes of Ex / Em=450 / 530nm and Ex / Em=541 / 590nm.

[0028] The heating device includes a heat sink 4 , a heating plate 5 fixedly disposed on the heat sink 4 , and a temperature sensor 6 fixedly disposed on the heat sink 4 . Both the temperature sensor 6 and the heating plate 5 are communicatively connected to a digital temperature control meter 7 .

[0029] The heating sheet 5 and the temperature sensor 6 are both adhered to the heat sink 4 through thermally conductive silica gel.

[0030] The heating sheet 5 is made of ceramic material.

[0031] The heat sink 4 is fixed on the bottom of the PCR tube 2, and the area around the PCR tube 2 is heated to the set temperature through the heat sink 4. When the heating button is pressed, the ceramic heating plate 5 is energized and heated, and the heat is dissipated to the area around the PCR tube 2 through the heat sink 4. When the temperature sensor 6 detects that the temperature reaches the set temperature upper limit (the default is 38°C), the digital temperature controller 7 automatically disconnects the power supply of the ceramic heating plate 5 and stops heating. The temperature drops until the set temperature lower limit (the default is 35°C), and the digital temperature controller 7 automatically connects the power supply of the ceramic heating plate 5, thereby controlling the ambient temperature around the PCR tube 2 to about 37°C.

[0032] The light intensity detection module is a light intensity meter, preferably a light intensity meter APP downloaded on a mobile phone. Press the excitation light switch (Ex=450 / 541nm), and the LED lamp 3 emits a monochromatic excitation light with a wavelength of 450nm or 540nm, which passes through the PCR tube 2 filled with reagents. Aim the mobile phone camera at the filter 11 installed in front of the PCR tube 2, and turn on the light intensity meter APP on the mobile phone to detect the light intensity value passing through the PCR tube 2, that is, fluorescence. The specific fluorescence measurement principle is: the mobile phone is sensitive to light through the camera, and the light intensity is fed back through the photoresistor integrated in the mobile phone (the greater the light intensity, the smaller the resistance value), so the voltage on the photoresistor changes with the resistance. According to the voltage-illuminance function relationship, the light intensity value is calculated from the voltage, and then output in the form of a visual interface for users to view the results.

[0033] The present invention uses specific examples to illustrate the principle and implementation of the present invention. The above examples are only used to help understand the method and core idea of ​​the present invention. At the same time, for those skilled in the art, according to the idea of ​​the present invention, there will be changes in the specific implementation and application scope. In summary, the content of this specification should not be understood as limiting the present invention.

Claims

1. A fluorescence detection device, characterized in that: It includes a shell, a plurality of detection holes are opened on the shell, filters are arranged at the detection holes, a plurality of detection stations are arranged inside the shell corresponding to the detection holes, a PCR tube and an LED lamp are arranged on each detection station, the light emitted by the LED lamp can pass through the PCR tube and the filter and project onto the light intensity detection module, a heating device capable of heating the PCR tube is arranged inside the shell, and a digital temperature control meter communicatively connected to the heating device is arranged inside the shell.

2. A fluorescence detection device according to claim 1, characterized in that: A bracket is fixedly arranged inside the shell, and the filter is fixedly arranged on the bracket.

3. A fluorescence detection device according to claim 1, characterized in that: The wavelength of the LED lamp is 450nm or 540nm.

4. A fluorescence detection device according to claim 1, characterized in that: The heating device comprises a heat sink, a heating plate fixedly arranged on the heat sink, and a temperature sensor fixedly arranged on the heat sink. Both the temperature sensor and the heating plate are communicatively connected with the digital temperature control meter.

5. A fluorescence detection device according to claim 4, characterized in that: The heating sheet and the temperature sensor are both adhered to the heat sink through thermally conductive silica gel.

6. A fluorescence detection device according to claim 4, characterized in that: The heating sheet is made of ceramic.

7. A fluorescence detection device according to claim 1, characterized in that: The light intensity detection module is a light intensity meter.

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