Homogenizing bag for aerobic bacteria culture

Abstract

The utility model belongs to a fungus culture technology, and discloses a homogenizing bag for aerobic bacteria culture, which is characterized in that an air inlet elbow is arranged on a bag body of the homogenizing bag, and a filter material and a plurality of air inlets are arranged on the air inlet elbow. On one hand, air entering the bag body through the air inlet elbow is secondarily filtered to achieve the purposes of improving the oxygen purity and reducing the pollutant entering amount, and on the other hand, the number of the air inlets can be adjusted according to needs to guarantee effective flowing and mixing of air in the homogenizing bag, and homogenizing uniformity is improved; therefore, by adopting the homogenizing bag disclosed by the utility model, the homogeneity and the purity of aerobic bacteria culture are improved, the accuracy and the reliability of experiments are ensured, and the problems in the prior art are solved.

Description

Technical Field

[0001] The utility model belongs to fungus culture technology, in particular to a homogenizing bag used for aerobic bacteria culture. Background Art

[0002] Homogenizing bags are usually used in experiments of culturing microorganisms or cells such as bacteria, fungi, and cells. Their main function is to provide a closed, sterile environment to protect the culture from contamination by the external environment and provide suitable growth conditions to promote the reproduction and growth of the culture. Common usage scenarios include experiments such as bacterial culture, fungal culture, and cell culture.

[0003] Since aerobic bacteria have a large demand for oxygen, an air inlet is usually required on the homogenizing bag used for aerobic bacteria. Although the cultivation of microorganisms is usually carried out in the laboratory, it is impossible to achieve a completely sterile state. Therefore, some bacteria or particulate matter in the air will still enter the homogenizing bag and affect or contaminate the cultivation of aerobic bacteria.

[0004] Based on the above analysis, the present utility model application is to design a homogenizing bag for aerobic bacteria culture. Utility Model Content

[0005] The utility model aims to provide a homogenizing bag for aerobic bacteria culture, so as to solve the problem that during the existing aerobic bacteria culture process, pollutants in the air easily enter the homogenizing bag through the air inlet 5 on the homogenizing bag and then contaminate the aerobic bacteria culture.

[0006] In order to solve the above problems, the utility model provides a homogenizing bag for aerobic bacteria culture, including a bag body, which is hot-pressed on all sides, and a sealing strip and an easy-tear opening are provided on the bag body near the upper end. An air inlet bend is provided on one side of the bag body, and the air inlet bend is a multi-section continuous bent tube, and a filter material is provided in the air inlet bend. At the same time, at least two air inlets are provided on the air inlet bend, and the two air inlets are located at different positions of the air inlet bend and a cap is provided on the air inlet.

[0007] As a preferred embodiment of the utility model, the filter material is graphene and nanofiber membrane, and the graphene and nanofiber membrane are respectively arranged at different positions of the air intake bend pipe, wherein the graphene filter material is arranged at a section of the air intake bend pipe away from the bag body, and the nanofiber membrane filter material is arranged at a section of the air intake bend pipe close to the bag body.

[0008] As a preferred embodiment of the utility model, one of the two air inlets is located on the air inlet elbow between the graphene filter material and the nanofiber membrane filter material, and the other air inlet is located at the end of the air inlet elbow.

[0009] As a preferred embodiment of the utility model, the diameter of the air intake elbow is 0.2-0.5 mm.

[0010] As a preferred embodiment of the utility model, the outer walls on both sides of the bag body are symmetrically provided with a support frame capable of supporting the bag body, and the support frame includes a first support arm and a second support arm, the length of the first support arm is greater than the length of the second support arm, one end of the first support arm is fixed to the side wall of the bag body, and the other end is connected to one end of the second support arm to form an L-shaped tripod structure.

[0011] As a preferred embodiment of the present utility model, the end of the first support arm is fixed to the side wall of the bag body by heat pressing or detachably fixed by a connecting piece.

[0012] As a preferred embodiment of the present utility model, when the end of the first support arm is detachably fixed to the bag body via a connecting piece, the connecting piece is a snap button or a magnetic buckle.

[0013] As a preferred embodiment of the present utility model, the first support arm is hinged to the second support arm, and the second support arm can rotate around the hinge point as an axis to achieve folding and extension relative to the first support arm.

[0014] Compared with the existing technology, the advantages of this homogenizing bag are:

[0015] The utility model provides an air inlet elbow on the bag body of the homogenizing bag, and provides filter materials and a plurality of air inlets on the air inlet elbow. On the one hand, secondary filtration of the air entering the bag body through the air inlet elbow is achieved to achieve the purpose of improving the oxygen purity and reducing the amount of pollutants entering. On the other hand, the number of air inlets can be adjusted as needed to ensure the effective flow and mixing of the gas in the homogenizing bag, thereby improving the homogenization uniformity. Therefore, the use of the homogenizing bag improves the homogeneity and purity of aerobic bacteria culture, ensures the accuracy and reliability of the experiment, and solves the problems existing in the prior art. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1 It is a partial cross-sectional structural schematic diagram of the homogenizing bag of the utility model.

[0017] Figure 2 It is a schematic diagram of the main structure of the homogenizing bag of the utility model.

[0018] Figure 3 It is a schematic diagram of the side structure of the homogenizing bag of the utility model.

[0019] Figure 4 For the utility model embodiment Figure 3 A local enlarged schematic diagram of point A in the middle.

[0020] Attached photos

[0021] 1 is the bag body 1; 2 is the sealing strip; 3 is the tearable opening; 4 is the air inlet elbow; 5 is the air inlet; 6 is the filter material; 7 is the diaphragm; 8 is the support frame; 81 is the first support arm; 82 is the second support arm; 83 is the hinge shaft. DETAILED DESCRIPTION

[0022] The present invention will be further described in detail below in conjunction with specific implementations and with reference to the accompanying drawings. It should be emphasized that the following description is merely exemplary and is not intended to limit the scope of the present invention and its application.

[0023] like Figure 1 As shown, a homogenizing bag for aerobic bacteria culture provided in this embodiment comprises a bag body 1, and the bag body 1 is a sealing structure formed by hot pressing around two pieces of bag materials. A sealing strip 2 and an easy-tear opening 3 are provided on the bag body 1 near the upper end. It can be understood that the easy-tear opening 3 should be located above the sealing strip 2, and the bag body 1 with an upper end opening can be formed by tearing along the easy-tear opening 3, and the bag body 1 can be opened and closed by the sealing strip 2; in this embodiment, a diaphragm 7 is provided in the bag body 1 of the homogenizing bag, and the diaphragm 7 is used to facilitate filtering and obtaining the culture solution.

[0024] An air inlet bend 4 is provided on one side of the bag body 1, and the diameter of the air inlet bend 4 is 0.2-0.5 mm. It can be understood that the air inlet bend 4 should be arranged at a position close to the upper end of the bag body 1 to prevent the culture solution from flowing out of the air inlet bend 4. The air inlet bend 4 is a multi-section continuous bend tube, that is, it is composed of a plurality of sections of continuous bends, and a filter material 6 is provided in the air inlet bend 4. In this embodiment, the filter material 6 can be provided in one place in the air inlet bend 4, or multiple places can be provided. At the same time, at least two air inlets 5 are provided on the air inlet bend 4, and the two air inlets 5 are located at different positions of the air inlet bend 4 and a cover is provided on the air inlet 5. Cap, since the air intake volume of a single air inlet 5 is limited, in order to ensure uniform circulation of gas in the homogenizing bag and improve the cultivation efficiency of microorganisms, multiple air inlets 5 can be opened at the same time when necessary, or one of the air inlets 5 can be opened according to the air index in the laboratory. Specifically, when the air index in the laboratory is average, the air inlet 5 farthest from the bag body 1 can be opened at this time, so that the air can be filtered twice through the filter material 6 in the air inlet elbow 4 after entering, avoiding the influence on the aerobic bacteria culture structure. When the air index in the laboratory is good, the air inlet 5 closest to the bag body 1 can be opened to increase the air intake speed.

[0025] In this embodiment, the preferred filter material 6 includes graphene and nanofiber membrane, which are respectively arranged at different positions of the air intake bend 4, that is, multiple filter materials 6 are arranged in the air intake bend 4, wherein the graphene filter material 6 is arranged at a section of the air intake bend 4 away from the bag body 1, and the nanofiber membrane filter material 6 is arranged at a section of the air intake bend 4 close to the bag body 1. Setting the graphene filter material 6 at a position away from the bag body 1 is conducive to achieving better gas pollutant filtration and antibacterial effect, and the nanofiber membrane can filter tiny particles and bacteria. The double filtration not only improves the purity of oxygen entering the homogenizing bag, but also improves the purity of the bacteria in the culture medium, thereby avoiding the contamination of the culture by bacteria and particulate matter in the external environment.

[0026] In summary, this embodiment provides an air inlet elbow 4 on the bag body 1 of the homogenizing bag, and provides a filter material 6 and a plurality of air inlets 5 on the air inlet elbow 4. On the one hand, secondary filtration of the air entering the bag body 1 through the air inlet elbow 4 is achieved to achieve the purpose of improving the oxygen purity and reducing the amount of pollutants entering. On the other hand, the number of air inlets 5 can be adjusted as needed to ensure the effective flow and mixing of the gas in the homogenizing bag and improve the homogenization uniformity. Therefore, the homogenizing bag of this embodiment improves the homogeneity and purity of aerobic bacteria culture, ensures the accuracy and reliability of the experiment, and solves the problems existing in the prior art.

[0027] As a preferred embodiment of the utility model, support frames 8 capable of supporting the bag body 1 are symmetrically provided on the outer walls on both sides of the bag body 1. Figure 2-3 The support frame 8 can support the bag body 1 so that it can stand independently, preventing the bag body 1 from tipping over and causing the culture solution to overflow from the air inlet 5 and affect the flow of gas in the bag body 1; the support frame 8 includes a first support arm 81 and a second support arm 82, the length of the first support arm 81 is greater than the length of the second support arm 82, one end of the first support arm 81 is fixed to the side wall of the bag body 1, and the other end is connected to one end of the second support arm 82 to form an L-shaped tripod structure. It can be understood that the second support arm 82 should be in contact with the bottom of the bag body 1, and the second support arm 82 cooperates with the first support arm 81 to support the main body of the bag body 1, thereby assisting the bag body 1 to stand.

[0028] It can be understood that one end of the first support arm 81 should be set near the middle or upper end of the bag body 1, so as to achieve a better supporting effect. In this embodiment, the connection position between the first support arm 81 and the bag body 1 is preferably located 3-5 cm above the liquid level in the bag body 1.

[0029] The end of the first support arm 81 is fixed to the side wall of the bag body 1 by heat pressing or detachably fixed by a connecting piece. The hot pressing fixing method does not require installation and is convenient for direct use. The detachable fixing method using the connecting piece is conducive to the disassembly, assembly and storage of the support frame 8.

[0030] When the connection is detachable via a connector, the connector may be a snap button or a magnetic button.

[0031] In order to facilitate the storage of the support frame 8 in this embodiment, one end of the first support arm 81 is preferably fixed to the side wall of the bag body 1 by heat pressing, and the other end is hinged to the second support arm 82 through a hinge shaft 83. The second support arm 82 can rotate 90° around the hinge point to achieve folding and extension relative to the first support arm 81. Figure 4 As shown; it can be understood that the hinge shaft 83 is an existing conventional hinge structure, and the hinge shaft 83 is preferably made of plastic material.

[0032] When in use, the second support arm 82 can be rotated relative to the hinge shaft 83 to make it horizontal. At this time, it contacts the bottom of the bag body 1 and cooperates with the first support arm 81 to support the bag body 1. The support of the support frame 8 can assist the bag body 1 to stand independently. On the one hand, it can improve the stability of the bag body 1 and prevent the bag body 1 from tipping over and causing the culture solution to flow out from the air inlet 5. On the other hand, it can also improve the three-dimensionality of the bag body 1, facilitate air entry to achieve effective gas flow, improve the culture effect of the culture solution in the homogenous bag, and at the same time, facilitate subsequent liquid extraction and other operations.

[0033] The above is only an embodiment of the utility model, and the common knowledge such as the known specific structure and characteristics in the scheme is not described in detail here. It should be pointed out that for those skilled in the art, several improvements can be made without departing from the premise of the utility model, which should also be regarded as the protection scope of the utility model, and these will not affect the effect of the implementation of the utility model and the practicality of the patent. The protection scope required by the utility model shall be based on the content of the claims, and the specific implementation methods and other records in the specification can be used to interpret the content of the claims.

Claims

1. A homogenizing bag for aerobic bacteria culture, comprising a bag body, the four sides of which are heat-pressed, and a sealing strip and an easy-tear opening are provided on the bag body near the upper end, characterized in that: An air intake bend is provided on one side of the bag body. The air intake bend is a multi-section continuous bent tube, and a filter material is provided inside the air intake bend. At the same time, at least two air inlets are provided on the air intake bend, and the two air inlets are located at different positions of the air intake bend and a cap is provided on the air inlet.

2. The homogenizing bag for aerobic bacteria culture according to claim 1, characterized in that: The filter material includes graphene and nanofiber membrane, which are respectively arranged at different positions of the air intake bend, wherein the graphene filter material is arranged at a section of the air intake bend away from the bag body, and the nanofiber membrane filter material is arranged at a section of the air intake bend close to the bag body.

3. The homogenizing bag for aerobic bacteria culture according to claim 2, characterized in that: One of the two air inlets is located on the air inlet elbow between the graphene filter material and the nanofiber membrane filter material, and the other air inlet is located at the end of the air inlet elbow.

4. The homogenizing bag for aerobic bacteria culture according to claim 1, characterized in that: The diameter of the air intake elbow is 0.2-0.5 mm.

5. The homogenizing bag for aerobic bacteria culture according to claim 1, characterized in that: The outer walls on both sides of the bag body are symmetrically provided with a support frame that can support the bag body, and the support frame includes a first support arm and a second support arm. The length of the first support arm is greater than the length of the second support arm. One end of the first support arm is fixed to the side wall of the bag body, and the other end is connected to one end of the second support arm to form an L-shaped tripod structure.

6. The homogenizing bag for aerobic bacteria culture according to claim 5, characterized in that: The end of the first support arm is fixed to the side wall of the bag body by heat pressing or detachably fixed by a connecting piece.

7. The homogenizing bag for aerobic bacteria culture according to claim 6, characterized in that: When the end of the first support arm is detachably fixed to the bag body via a connecting piece, the connecting piece is a snap button or a magnetic button.

8. The homogenizing bag for aerobic bacteria culture according to claim 5, characterized in that: The first support arm is hinged to the second support arm, and the second support arm can rotate around the hinge point as an axis to achieve folding and extension relative to the first support arm.

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