Organ-like section antibody incubation wet box

By using an inclined support structure and lighting device in the incubation box of the organoid section antibody, the problem of easy damage to the slides and difficulty in observation during the liquid absorption process is solved, and the convenience of liquid absorption and safety protection of the slice are achieved.

CN222299632UActive Publication Date: 2025-01-03THE FIRST MEDICAL CENT CHINESE PLA GENERAL HOSPITAL
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Patent Information

Application Number
CN202420882934.1
Authority / Receiving Office
CN · China
Patent Type
Utility models(China)
Current Assignee / Owner
Filing Date
2024-04-26
Publication Date
2025-01-03
Estimated Expiration
2034-04-26

AI Technical Summary

Technical Problem

During the liquid aspiration process of existing organoid section antibody incubation box, the slides are easily touched by the pipette, causing damage, and the liquid aspiration is inconvenient, making it difficult to effectively observe the position of the section.

Method used

A wet box of organoid slice antibodies incubation is designed, using an inclined support structure and lighting device, providing a liquid suction tank by operating the inclined partition and support plate in the compartment, combining lighting rods and LED lamp beads to ensure that the slides are in Place it inclined and can be clearly observed to avoid damage to the gun head.

Benefits of technology

The convenience of liquid ablation and safety protection of organoid slices are achieved, which avoids accidental damage and improves the visibility of the operation.

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Abstract

The utility model provides an organ-like section antibody incubation wet box. An operation compartment is integrally formed outside the side wall of one end of the organ-like section antibody incubation wet box; an inclined partition plate is formed in the middle of the operation compartment; a through hole is formed in the side wall; the through hole is located above the partition plate. A left supporting plate and a right supporting plate are formed on the partition plate in an upward extending mode. And inclined glass slide placing grooves are formed at the upper ends of the left supporting plate and the right supporting plate. According to the organ-like section antibody incubation wet box, glass slides can be placed in the glass slide placement grooves of the operation compartments during liquid suction, so that the glass slides are inclined, and the liquid suction operation is facilitated.
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Description

Technical Field

[0001] The present application relates to a medical experimental device, and in particular to an organoid slice antibody incubation wet box. Background Art

[0002] In the prior art, the organoid slice antibody incubation wet box 10 is as follows Figure 1 As shown, during the immunohistochemistry or immunofluorescence process of organoid slices, it is necessary to remove the liquid on the slide to recover the antibody liquid. The existing slide is placed horizontally on the organoid slice antibody incubation wet box, which is not conducive to the removal of liquid. Moreover, the organoid slices are very small, and during the process of removing the liquid, they are easily hit by the tip of the pipette, causing damage to the organoid slices. Utility Model Content

[0003] In view of the above problems, the present application aims to propose an organoid slice antibody incubation wet box, which can provide an inclined support for the slide during the liquid aspiration step, thereby facilitating the aspiration of liquid during the operation; the present application also aims to propose an organoid slice antibody incubation wet box, which is convenient for observing the position of the organoid slice during the liquid aspiration process, thereby avoiding accidental damage to the organoid slice by the pipette tip.

[0004] The organoid slice antibody incubation wet box of the present application has an operation compartment integrally formed outside the side wall at one end thereof; an inclined partition is formed in the middle of the operation compartment; a through hole is formed on the side wall; the through hole is located above the partition; a left support plate and a right support plate are formed on the partition extending upward; an inclined slide placement groove is formed at the upper ends of the left support plate and the right support plate.

[0005] Preferably, the partition is transparent;

[0006] A left plug hole is formed on the left wall of the operating compartment, and a right plug hole is formed on the right wall of the operating compartment; the left plug hole and the right plug hole are aligned; the lighting rod can be plugged in and out of the left plug hole and the right plug hole; the lighting rod is located below the partition;

[0007] At least one LED lamp bead is formed on the upper side of the lighting stick; a battery is installed inside the lighting stick; a switch is also installed on the lighting stick; the battery, the LED lamp bead and the switch are connected in series to form a power supply circuit.

[0008] Preferably, a plurality of steps are formed at the lower ends of the glass slide placement grooves of the left support plate and the right support plate.

[0009] The organoid slice antibody incubation wet box of the present application can place the slide in the slide placement slot in the operation compartment when the liquid is sucked out, so that the slide is tilted to facilitate the liquid suction operation; a lighting rod is arranged under the partition to facilitate the operator to observe the organoid slices on the slide and avoid accidental stabbing of the organoid slices by the tip of the pipette. BRIEF DESCRIPTION OF THE DRAWINGS

[0010] Figure 1 A schematic diagram of the structure of an organoid slice antibody incubation wet box in the prior art;

[0011] Figure 2 This is a schematic diagram of the structure of the organoid slice antibody incubation wet box of the present application;

[0012] Figure 3 A schematic diagram of the organoid slice antibody incubation humid chamber of the present application after removing the illumination rod;

[0013] Figure 4 for Figure 3 Schematic diagram of the cross-section of the wet box for antibody incubation of organoid slices;

[0014] Figure 5 for Figure 3 A partial enlarged schematic diagram of the step portion of the antibody incubation humidified box for organoid slices;

[0015] Figure 6 Schematic diagram of the illumination bar in the humidified chamber for antibody incubation of organoid slices according to the present application. DETAILED DESCRIPTION

[0016] Below, the organoid slice antibody incubation wet box of the present application is described in detail with reference to the accompanying drawings.

[0017] The organoid slice antibody incubation wet box 10 of the present application has an operation compartment 11 integrally formed outside the side wall at one end thereof.

[0018] An inclined partition 13 is formed in the middle of the operating compartment.

[0019] A through hole 16 is formed on the side wall, so that the liquid on the partition 13 flows through the through hole into the organoid slice antibody incubation humid box 10. The through hole 16 is located above the partition 13 and is adjacent to the partition.

[0020] A left support plate 12a and a right support plate 12b are formed on the partition 13 to extend upward; an inclined slide placement groove is formed at the upper end of the left support plate 12a and the right support plate 12b to facilitate the inclined placement of the slide and to facilitate the use of a pipette to remove the liquid on the slide.

[0021] The partition 13 is transparent; a left plug hole 15a is formed on the left wall of the operating compartment 11, and a right plug hole 15b is formed on the right wall of the operating compartment 11; the left plug hole 15a and the right plug hole 15b are aligned; the lighting rod 20 can be plugged in and out of the left plug hole 15a and the right plug hole 15b; the lighting rod 20 is located below the partition 13 to provide lighting to facilitate the operator to see the position of the organoid slice on the slide, so as to avoid the pipette accidentally stabbing the organoid slice during the operation of sucking out the liquid.

[0022] At least one LED lamp bead 22 is formed on the upper side of the lighting stick 20; a battery (not shown in the figure) is installed inside the lighting stick 20; a switch 21 is also installed on the lighting stick 20; the battery, the LED lamp bead 22, and the switch 21 are connected in series to form a power supply circuit.

[0023] In order to adjust the inclination angle of the slide, a plurality of steps 17 may be formed at the lower ends of the slide placement grooves of the left support plate 12a and the right support plate 12b. Figure 5 Thus, by supporting the lower edge of the slide on different steps, the degree of inclination of the slide can be changed.

[0024] When in use, place the slide in the slide support groove and select the appropriate step so that the slide is tilted at an angle suitable for the operator to remove the liquid. Press the switch to turn on the power supply circuit of the lighting stick, and the LED lamp beads will emit light under the partition; the light will illuminate the slide through the transparent partition, so that the operator can clearly see the position of the organoid slices and avoid the pipette tip accidentally stabbing the organoid slices when removing the liquid. Due to the existence of the partition, even if liquid drips, it will not cause a short circuit to the lighting stick.

Claims

1. A wet box for incubating organoid slices with antibodies, characterized in that: An operating compartment is integrally formed outside the side wall at one end thereof; an inclined partition is formed in the middle of the operating compartment; a through hole is formed on the side wall; the through hole is located above the partition; a left support plate and a right support plate are formed on the partition extending upward; an inclined slide placement groove is formed at the upper ends of the left support plate and the right support plate.

2. The organoid slice antibody incubation humid chamber according to claim 1, characterized in that: The partition is transparent; A left plug hole is formed on the left wall of the operating compartment, and a right plug hole is formed on the right wall of the operating compartment; the left plug hole and the right plug hole are aligned; The lighting rod can be plugged into the left and right sockets; the lighting rod is located below the partition; At least one LED lamp bead is formed on the upper side of the lighting stick; a battery is installed inside the lighting stick; a switch is also installed on the lighting stick; the battery, the LED lamp bead and the switch are connected in series to form a power supply circuit.

3. The organoid slice antibody incubation humid chamber according to claim 1, characterized in that: A plurality of steps are formed at the lower ends of the glass slide placement grooves of the left supporting plate and the right supporting plate.