Adding device of hematopoietic stem cell cryopreservation protective agent

Abstract

The utility model discloses an adding device of a hematopoietic stem cell cryopreservation protective agent, and relates to the technical field of hematopoietic stem cells, the adding device comprises an adding platform, the front side and the rear side of the top of the adding platform are fixedly provided with shaking-up positioning mechanisms which are arranged in a mirror image mode, and the center of the top of the adding platform is fixedly provided with an injection mechanism. The shaking-up positioning mechanism on the front side comprises a fixing box, the fixing box is fixedly mounted at the top of the adding platform, a positioning flat plate is arranged at the top of the fixing box, three positioning mechanisms are fixedly mounted at the top of the positioning flat plate, and three vertical plates are fixedly mounted on the rear side of the top of the positioning flat plate. According to the utility model, through the mutual cooperation of the fixing box, the positioning flat plate, the positioning mechanism, the turntable and the convex block, the blood bag fixed on the positioning mechanism can be vibrated and extruded during injection, so that a cryopreservation protective agent added into the hematopoietic stem cell blood bag is uniformly mixed with hematopoietic stem cells; and the probability of damage during later low-temperature storage is reduced.

Description

Technical Field

[0001] The utility model relates to the technical field of hematopoietic stem cells, in particular to a device for adding a hematopoietic stem cell cryopreservation protective agent. Background Art

[0002] In order to extract hematopoietic stem cells from umbilical cord blood, it is necessary to conduct a separation experiment on the umbilical cord blood, and the stem cells finally extracted after separation need to be stored in liquid nitrogen at -196°C at a deep cryogenic temperature. In order to protect the cells from damage in a low temperature environment, a cryopreservation fluid needs to be added to the hematopoietic stem cells from the umbilical cord blood as a protective agent for the hematopoietic stem cells from the umbilical cord blood. The process of adding the cryopreservation fluid requires slow and uniform addition, and the blood bag should be shaken during the addition process to fully mix the cryopreservation fluid with the hematopoietic stem cells from the umbilical cord blood. The existing technology has the following problems:

[0003] Because the existing cryoprotectant addition device uses a single injection mechanism for injection, the blood bag storing hematopoietic stem cells cannot be shaken evenly during injection, resulting in uneven mixing between the cryoprotectant and the hematopoietic stem cells, poor mixing effect, and easy to cause accidental damage to the hematopoietic stem cells at low temperatures. Utility Model Content

[0004] The utility model provides a device for adding a hematopoietic stem cell cryopreservation protective agent to solve the problems raised in the above background technology.

[0005] In order to solve the above technical problems, the technical solution adopted by the utility model is:

[0006] A device for adding a hematopoietic stem cell cryopreservation protective agent comprises an adding platform, mirror-imaged shaking and positioning mechanisms are fixedly installed on the front and rear sides of the top of the adding platform, an injection mechanism is fixedly installed at the top center of the adding platform, the front shaking and positioning mechanism comprises a fixing box, the fixing box is fixedly installed on the top of the adding platform, a positioning plate is arranged on the top of the fixing box, three positioning mechanisms are fixedly installed on the top of the positioning plate, three vertical plates are fixedly installed on the rear side of the top of the positioning plate, driving motors are fixedly installed on the rear sides of the three vertical plates, the output shafts of the three driving motors respectively penetrate the front sides of the three vertical plates and are fixedly installed with turntables, and a plurality of protrusions are fixedly installed on the front sides of the three turntables.

[0007] A further improvement of the technical solution of the utility model is that the three positioning mechanisms on the front side respectively include two columns, a fixed cross plate is fixedly installed on the rear side of the top ends of the two columns, a knob is movably installed on the left side of the rear end of the three fixed cross plates, the front ends of the three knobs penetrate to the front side of the fixed cross plate and are fixedly installed with threaded rods, the outer walls of the three threaded rods are threadedly installed with clamping plates, the front ends of the three threaded rods are fixedly installed with limiting disks, the right sides of the front ends of the three fixed cross plates are fixedly installed with sliding rods, the sliding rods are slidably connected to the clamping plates, and the opposite surfaces of the three fixed cross plates and the three clamping plates are respectively provided with bag clamping grooves.

[0008] A further improvement of the technical solution of the utility model lies in that: a vibration motor is fixedly installed at the center of the bottom inner wall of the fixed box on the front side, a knocking plate is fixedly installed on the output shaft of the vibration motor, a knocking block is fixedly installed on the bottom of the knocking plate, telescopic rods are fixedly installed on the left and right sides of the bottom inner wall of the fixed box, the top ends of the two telescopic rods are fixedly connected to the bottom of the positioning plate, springs are arranged on the left and right sides of the vibration motor, the upper and lower ends of the two springs are respectively fixedly connected to the bottom of the positioning plate and the bottom of the inner wall of the fixed box, and the top of the knocking plate overlaps the bottom of the positioning plate.

[0009] A further improvement of the technical solution of the utility model is that the injection mechanism includes a base plate, the base plate is fixedly installed at the top center of the adding platform, a motor slot is opened at the top center of the base plate, and a rotating motor is fixedly installed in the motor slot, the output shaft of the rotating motor is fixedly installed with a bottom plate, the top of the bottom plate is fixedly installed with an electric push rod, and the top of the electric push rod is fixedly installed with a hanging top plate.

[0010] A further improvement of the technical solution of the utility model lies in that: a cryoprotectant storage tank is fixedly installed on the top of the hanging top plate, a liquid pump is fixedly installed on the top of the front horizontal part of the hanging top plate, the input end of the liquid pump is connected with the inner cavity of the cryoprotectant storage tank, the output end of the liquid pump is fixedly connected with an infusion tube, a liquid storage box is fixedly installed on the bottom of the front horizontal part of the hanging top plate, the output end of the infusion tube passes through the bottom of the front horizontal part of the hanging top plate and is connected with the inner cavity of the liquid storage box, and three injection tubes are evenly fixedly installed on the bottom of the liquid storage box, and the three injection tubes are respectively located above the three positioning mechanisms on the front side.

[0011] Due to the adoption of the above technical solution, the utility model has achieved the following technical progress compared with the prior art:

[0012] 1. The utility model provides a device for adding a cryopreservation protective agent for hematopoietic stem cells. Through the cooperation of a fixing box, a positioning plate, a positioning mechanism, a turntable, and a convex block, a blood bag fixed on the positioning mechanism can be vibrated and squeezed during injection, so that the cryopreservation protective agent added to the hematopoietic stem cell blood bag is evenly mixed with the hematopoietic stem cells, reducing the probability of damage during later low-temperature storage.

[0013] 2. The utility model provides a device for adding cryopreservation protective agents for hematopoietic stem cells. Through the cooperation between the positioning mechanism and the injection mechanism, the blood bags fixed on the positioning mechanisms on the front and rear sides of the adding platform can be alternately added with cryopreservation protective agents. At the same time, while adding blood bags on one side, the blood bags on the other side can be replaced, thereby improving the efficiency of adding cryopreservation protective agents to hematopoietic stem cells. BRIEF DESCRIPTION OF THE DRAWINGS

[0014] Figure 1 It is an overall schematic diagram of the structure of the utility model;

[0015] Figure 2 It is a schematic diagram of the shaking and positioning mechanism of the utility model structure;

[0016] Figure 3 It is a schematic diagram of the positioning mechanism of the utility model structure;

[0017] Figure 4 It is a schematic diagram of the interior of the fixing box of the utility model structure;

[0018] Figure 5 It is a schematic diagram of the injection mechanism of the utility model structure.

[0019] In the figure: 1. Adding platform; 2. Shaking positioning mechanism; 21. Fixed box; 211. Vibrating motor; 212. Knocking plate; 213. Knocking block; 214. Spring; 215. Telescopic rod; 22. Positioning plate; 23. Vertical plate; 24. Driving motor; 25. Turntable; 26. Bump; 27. Positioning mechanism; 271. Vertical column; 272. Fixed horizontal plate; 273. Knob; 274. Threaded rod; 275. Limiting plate; 276. Clamping plate; 277. Sliding rod; 278. Bag mouth clamping groove; 3. Injection mechanism; 31. Base plate; 32. Rotating motor; 33. Bottom plate; 34. Electric push rod; 35. Suspended top plate; 351. Liquid storage box; 352. Injection tube; 36. Cryoprotectant storage tank; 37. Liquid pump; 371. Infusion tube. DETAILED DESCRIPTION

[0020] In order to make the technical means, creative features, objectives and effects achieved by the present invention easy to understand, the present invention is further described below in conjunction with specific implementation methods.

[0021] like Figure 1 , Figure 2 , Figure 3 As shown, the utility model provides a device for adding a hematopoietic stem cell cryopreservation protective agent, comprising an adding platform 1, mirror-imaged shaking and positioning mechanisms 2 are fixedly installed on the front and rear sides of the top of the adding platform 1, an injection mechanism 3 is fixedly installed at the top center of the adding platform 1, the front shaking and positioning mechanism 2 comprises a fixing box 21, the fixing box 21 is fixedly installed on the top of the adding platform 1, a positioning plate 22 is arranged on the top of the fixing box 21, three positioning mechanisms 27 are fixedly installed on the top of the positioning plate 22, three vertical plates 23 are fixedly installed on the rear side of the top of the positioning plate 22, and driving motors 24 are fixedly installed on the rear sides of the three vertical plates 23, and the output shafts of the three driving motors 24 respectively penetrate the front sides of the three vertical plates 23 and are fixedly installed with turntables 25, and the three turntables A plurality of protrusions 26 are fixedly installed on the front side of each of the two upright posts 271. The three positioning mechanisms 27 on the front side respectively include two upright posts 271. A fixed horizontal plate 272 is fixedly installed on the rear side of the top ends of the two upright posts 271. A knob 273 is movably installed on the left side of the rear ends of the three fixed horizontal plates 272. The front ends of the three knobs 273 all penetrate the front side of the fixed horizontal plate 272 and are fixedly installed with a threaded rod 274. The outer walls of the three threaded rods 274 are threadedly installed with a clamping plate 276. The front ends of the three threaded rods 274 are fixedly installed with a limiting disk 275. The right sides of the front ends of the three fixed horizontal plates 272 are fixedly installed with a sliding rod 277. The sliding rod 277 is slidably connected with the clamping plate 276. The opposite surfaces of the three fixed horizontal plates 272 and the three clamping plates 276 are respectively provided with a bag opening clamping groove 278.

[0022] By moving the three blood bags containing hematopoietic stem cells upward between the two columns 271 included in the three positioning mechanisms 27 on the front side, and making the hard plastic injection port of the blood bag between the bag opening clamping groove 278 opened on the opposite sides of the fixed horizontal plate 272 and the clamping plate 276, and then rotating the knob 273 to drive the threaded rod 274 to rotate, and utilizing the threaded connection between the threaded rod 274 and the clamping plate 276, the clamping plate 276 is stably moved toward the fixed horizontal plate 272 using the sliding rod 277, until the hard plastic injection port of the blood bag is firmly clamped by the bag opening clamping groove 278, and at the same time, one side of the blood bag will stick to several protrusions 26 on the front side of the turntable 25, and then the turntable 25 can be driven to rotate by starting the driving motor 24 on the rear side of the vertical plate 23, so that the protrusions 26 squeeze the blood bag containing hematopoietic stem cells to achieve internal mixing.

[0023] like Figure 4As shown, a vibration motor 211 is fixedly installed at the center of the bottom of the inner wall of the front fixed box 21, a knocking plate 212 is fixedly installed on the output shaft of the vibration motor 211, a knocking block 213 is fixedly installed on the bottom of the knocking plate 212, telescopic rods 215 are fixedly installed on the left and right sides of the bottom of the inner wall of the fixed box 21, the tops of the two telescopic rods 215 are fixedly connected to the bottom of the positioning plate 22, springs 214 are arranged on the left and right sides of the vibration motor 211, the upper and lower ends of the two springs 214 are fixedly connected to the bottom of the positioning plate 22 and the bottom of the inner wall of the fixed box 21, and the top of the knocking plate 212 overlaps the bottom of the positioning plate 22;

[0024] At the same time, when the cryopreservative protective agent is injected, the vibration motor 211 in the fixed box 21 is used to drive the knocking plate 212 to rotate, and the knocking block 213 at the bottom of the knocking plate 212 is used to repeatedly knock the top positioning plate 22, so that it vibrates up and down using the spring 214, and at the same time cooperates with the two telescopic rods 215 to maintain the stability of the up and down vibration, so that the hematopoietic stem cells in the blood bag and the cryopreservative protective agent can be mixed more evenly using vibration.

[0025] like Figure 5 As shown, the injection mechanism 3 includes a base plate 31, the base plate 31 is fixedly installed at the top center of the adding platform 1, a motor slot is opened at the top center of the base plate 31, and a rotating motor 32 is fixedly installed in the motor slot, a bottom plate 33 is fixedly installed on the output shaft of the rotating motor 32, an electric push rod 34 is fixedly installed on the top of the bottom plate 33, a hanging top plate 35 is fixedly installed on the top of the electric push rod 34, a cryoprotectant storage tank 36 is fixedly installed on the top of the hanging top plate 35, and a cryoprotectant storage tank 36 is fixedly installed on the top of the front side horizontal surface of the hanging top plate 35. A liquid pump 37 is installed, the input end of the liquid pump 37 is connected to the inner cavity of the cryoprotectant storage tank 36, the output end of the liquid pump 37 is fixedly connected to an infusion tube 371, a liquid storage box 351 is fixedly installed at the bottom of the front horizontal side of the suspension top plate 35, the output end of the infusion tube 371 penetrates to the bottom of the front horizontal side of the suspension top plate 35 and is connected to the inner cavity of the liquid storage box 351, and three injection tubes 352 are evenly fixedly installed at the bottom of the liquid storage box 351, and the three injection tubes 352 are respectively located above the three positioning mechanisms 27 on the front side;

[0026] During injection, the electric push rod 34 on the top of the bottom plate 33 can be started to drive the hanging top plate 35 and the liquid storage box 351 to descend, so that the three injection tubes 352 are respectively inserted into the three blood bag injection ports fixed on the front side, and the cryoprotectant in the cryoprotectant storage tank 36 is extracted by the liquid pump 37, and injected into the inner cavity of the liquid storage box 351 through the infusion tube 371, and finally injected into the three blood bags by the three injection tubes 352. After the injection is completed, the electric push rod 34 can be driven to drive the hanging top plate 35 to move upward. Thereby, the injection tube 352 pulls out the blood bag, and then the rotating motor 32 in the base plate 31 is started to drive the overall bottom plate 33 and the hanging top plate 35 on the top electric push rod 34 to rotate 180 degrees, so that the three injection tubes 352 are aligned with the top of the three positioning mechanisms 27 on the rear side, and the three blood bags fixed on the rear side are injected. At this time, the blood bag injected on the front side can be removed and replaced. After the blood bag on the rear side is injected, the injection tube 352 is rotated back to the front side for injection, and the cryoprotectant is quickly added in this way.

[0027] The working principle of the device for adding the hematopoietic stem cell cryopreservation protective agent is described in detail below.

[0028] like Figure 1-5As shown, three blood bags containing hematopoietic stem cells are moved upward between two upright posts 271 included in the three front positioning mechanisms 27, and the hard plastic injection port of the blood bag is located between the bag opening clamping groove 278 provided on the opposite sides of the fixed horizontal plate 272 and the clamping plate 276, and then the knob 273 is rotated to drive the threaded rod 274 to rotate, and the threaded connection between the threaded rod 274 and the clamping plate 276 is used to make the clamping plate 276 move toward the fixed horizontal plate 272 stably by the sliding rod 277, until the hard plastic injection port of the blood bag is firmly clamped by the bag opening clamping groove 278. At the same time, one side of the blood bag will stick to several protrusions 26 on the front side of the turntable 25, and then the turntable 25 can be driven to rotate by starting the driving motor 24 on the rear side of the vertical plate 23, so that the protrusions 26 squeeze the blood bag containing hematopoietic stem cells to achieve internal mixing. When injecting, the electric push rod 34 on the top of the bottom plate 33 can be started to drive the hanging top plate 35 and the liquid storage box 351 to descend, so that the three injection tubes 352 are respectively inserted into the three blood bag injection ports fixed on the front side, and the cryoprotectant in the cryoprotectant storage tank 36 is extracted by the liquid extraction pump 37 and then injected into the blood bag 36. The liquid tube 371 is injected into the inner cavity of the liquid storage box 351, and finally the three injection tubes 352 are used to inject into the three blood bags respectively. After the injection is completed, the electric push rod 34 can be driven to drive the hanging top plate 35 to move upward, so that the injection tube 352 can pull out the blood bag, and then the rotating motor 32 in the base plate 31 is started to drive the overall bottom plate 33 and the hanging top plate 35 on the top electric push rod 34 to rotate 180 degrees, so that the three injection tubes 352 are aligned with the top of the three positioning mechanisms 27 on the rear side, and the three blood bags fixed on the rear side are injected. At this time, the blood bag injected on the front side can be taken out and put into When the cryoprotectant is injected, the vibration motor 211 in the fixed box 21 drives the knocking plate 212 to rotate, and the knocking block 213 at the bottom of the knocking plate 212 repeatedly knocks the top positioning plate 22, so that it vibrates up and down by the spring 214, and the two telescopic rods 215 maintain the stability of the up and down vibration, so that the hematopoietic stem cells in the blood bag and the cryoprotectant are mixed more evenly by vibration.

[0029] The above generally describes the present invention in detail, but it is obvious to a person skilled in the art that some modifications or improvements can be made to the present invention. Therefore, modifications or improvements that do not depart from the spirit of the present invention are within the scope of protection of the present invention.

Claims

1. A device for adding a hematopoietic stem cell cryoprotectant, comprising an adding platform (1), characterized in that: The top of the adding platform (1) is fixedly installed with mirror-imaged shaking and positioning mechanisms (2) on both front and rear sides, and the top center of the adding platform (1) is fixedly installed with an injection mechanism (3). The front shaking and positioning mechanism (2) includes a fixing box (21), and the fixing box (21) is fixedly installed on the top of the adding platform (1). A positioning plate (22) is arranged on the top of the fixing box (21), and three positioning mechanisms (27) are fixedly installed on the top of the positioning plate (22). Three vertical plates (23) are fixedly installed on the rear side of the top of the positioning plate (22), and driving motors (24) are fixedly installed on the rear sides of the three vertical plates (23). The output shafts of the three driving motors (24) respectively penetrate the front sides of the three vertical plates (23) and are fixedly installed with turntables (25), and the front sides of the three turntables (25) are fixedly installed with a plurality of protrusions (26).

2. The device for adding a hematopoietic stem cell cryoprotectant according to claim 1, characterized in that: The three positioning mechanisms (27) on the front side respectively include two upright posts (271), a fixed horizontal plate (272) is fixedly installed on the rear side of the top ends of the two upright posts (271), a knob (273) is movably installed on the left side of the rear ends of the three fixed horizontal plates (272), the front ends of the three knobs (273) all penetrate to the front side of the fixed horizontal plate (272) and are fixedly installed with a threaded rod (274), the outer walls of the three threaded rods (274) are threadedly installed with a clamping plate (276), the front ends of the three threaded rods (274) are fixedly installed with a limiting disk (275), the right sides of the front ends of the three fixed horizontal plates (272) are fixedly installed with a sliding rod (277), the sliding rod (277) is slidably connected with the clamping plate (276), and the opposite surfaces of the three fixed horizontal plates (272) and the three clamping plates (276) are respectively provided with bag opening clamping grooves (278).

3. The device for adding a hematopoietic stem cell cryoprotectant according to claim 1, characterized in that: A vibration motor (211) is fixedly mounted at the center of the bottom of the inner wall of the fixed box (21) at the front side, a knocking plate (212) is fixedly mounted on the output shaft of the vibration motor (211), a knocking block (213) is fixedly mounted at the bottom of the knocking plate (212), telescopic rods (215) are fixedly mounted on both left and right sides of the bottom of the inner wall of the fixed box (21), the top ends of the two telescopic rods (215) are fixedly connected to the bottom of the positioning plate (22), springs (214) are arranged on both left and right sides of the vibration motor (211), the upper and lower ends of the two springs (214) are fixedly connected to the bottom of the positioning plate (22) and the bottom of the inner wall of the fixed box (21), and the top of the knocking plate (212) overlaps the bottom of the positioning plate (22).

4. The device for adding a hematopoietic stem cell cryoprotectant according to claim 1, characterized in that: The injection mechanism (3) comprises a base plate (31), the base plate (31) is fixedly mounted at the top center of the adding platform (1), a motor slot is opened at the top center of the base plate (31), and a rotating motor (32) is fixedly mounted in the motor slot, the output shaft of the rotating motor (32) is fixedly mounted with a bottom plate (33), the top of the bottom plate (33) is fixedly mounted with an electric push rod (34), and the top of the electric push rod (34) is fixedly mounted with a hanging top plate (35).

5. The device for adding a hematopoietic stem cell cryoprotectant according to claim 4, characterized in that: A cryoprotectant storage tank (36) is fixedly installed on the top of the suspension top plate (35); a liquid pump (37) is fixedly installed on the top of the front horizontal part of the suspension top plate (35); the input end of the liquid pump (37) is connected to the inner cavity of the cryoprotectant storage tank (36); the output end of the liquid pump (37) is fixedly connected to an infusion tube (371); a liquid storage box (351) is fixedly installed on the bottom of the front horizontal part of the suspension top plate (35); the output end of the infusion tube (371) passes through the bottom of the front horizontal part of the suspension top plate (35) and is connected to the inner cavity of the liquid storage box (351); three injection tubes (352) are evenly fixedly installed on the bottom of the liquid storage box (351); the three injection tubes (352) are respectively located above the three positioning mechanisms (27) on the front side.

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