Electromagnetic microneedle device for cell sorting
By designing an electromagnetic microneedle device, using the magnetic permeable metal material and the magnetic force of the electromagnetic rod, the problem of inaccurate cell sorting in magnetic bead sorting is solved, and the precise selection of cells under a microscope is achieved, and cell sorting is improved. accuracy and purity.
Patent Information
- Application Number
- CN202323252213.7
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2023-11-30
- Publication Date
- 2025-01-07
- Estimated Expiration
- 2033-11-30
AI Technical Summary
In the existing cell sorting methods, although the magnetic bead sorting method is simple, the sorted cells are not accurate, making it difficult to achieve accurate cell sorting.
An electromagnetic microneedle device is designed, including microneedles and magnetic producing chambers. The microneedle is made of magnetic metal material, and is equipped with a magnetic coil, a battery pack and an electronically controlled switch. It generates magnetic force through the electromagnetic rod to accurately select cells.
The device is simple to operate, and combined with magnetic bead method, the precise selection of cells is achieved under a microscope, improving the accuracy and purity of cell sorting.
Smart Images
Figure CN222313182U_ABST
Abstract
Description
Technical Field
[0001] The utility model belongs to the technical field of microscope operating equipment, in particular to an electromagnetic micro-needle device for cell sorting. Background Art
[0002] Cell sorting is a technique that separates a specific cell subpopulation from a mixed cell sample based on the characteristics of the cell. It is an important topic in cytological research. When a specific cell needs to be functionally analyzed, such as detecting cell molecules through ELISA analysis of cell culture supernatant, detecting cell function through cell co-culture, etc., the premise is to obtain high-purity target cells. There are two commonly used cell sorting methods: flow cytometry and immunomagnetic cell sorting.
[0003] Immunomagnetic cell sorting is based on the property that cell surface antigens can bind to specific antibodies connected to magnetic beads. In an external magnetic field, cells connected to magnetic beads through antibodies are adsorbed and retained in the magnetic field, while cells without such surface antigens cannot bind to specific antibodies connected to magnetic beads and are not magnetic, so they do not stay in the magnetic field to achieve the purpose of cell separation.
[0004] The equipment required for magnetic bead cell sorting is simple and does not require high technical personnel. The sorted cells have high sensitivity, good purity, and good cell activity and recovery rate. It has little impact on downstream applications and has broad application prospects.
[0005] However, although the magnetic bead sorting method requires simple equipment, the sorted cells are not accurate. How to find a more ideal solution between these two methods, which can achieve accurate cell sorting without considering the cost of instruments and equipment. Summary of the invention
[0006] Technical solution: In order to solve the above-mentioned technical problems of cell sorting requirements, the utility model provides an electromagnetic microneedle device for cell sorting, which specifically includes a microneedle and a magnetic generating chamber; the microneedle is fixedly installed at the front end of the magnetic generating chamber; the magnetic generating chamber is a cylindrical structure, and a magnetic coil, a battery pack, and an electric control switch are installed inside; wherein the magnetic coil is electrically connected to the battery pack, and the battery pack is electrically connected to the electric control switch; the electric control switch is installed on the side wall of the cylindrical structure; the microneedle is a structure made of magnetically conductive metal material.
[0007] As an improvement, the needle size of the microneedle is 0.1um-1mm.
[0008] As an improvement, the battery pack supplies power to the electromagnetic rod, which generates magnetic force when powered on and disappears when powered off.
[0009] Beneficial effects: The device proposed by the utility model is easy to operate and combines the magnetic bead method to sort cells, so as to achieve the purpose of accurately selecting cells under a microscope. BRIEF DESCRIPTION OF THE DRAWINGS
[0010] Figure 1 This is a schematic diagram of the structure of the electromagnetic microneedle of the utility model.
[0011] Figure 2 This is a schematic diagram of the electromagnetic microneedle of the utility model being used under a microscope.
[0012] Figure 3 This is the field of view of the eyepiece of this practical magnetic microneedle microscope.
[0013] Figure 4 The utility model adopts the device to carry out the experimental data obtained by electrophoresis detection.
[0014] In the figure: 1 is a microneedle, 2 is a magnetic coil, 3 is a battery pack, 4 is an electric control switch, 5 is a microscope objective, 6 is a glass slide, 7 is a microscope stage structure, 8 is a magnetic bead carrier, and 9 is a carrier combined with magnetic beads. DETAILED DESCRIPTION
[0015] The following examples are used to illustrate the present invention, but are not intended to limit the scope of the present invention.
[0016] See Figure 1 As shown, the electromagnetic microneedle device for cell sorting specifically includes a microneedle 1 and a magnetic generating chamber; the microneedle 1 is fixedly installed at the front end of the magnetic generating chamber; the magnetic generating chamber is a cylindrical structure, and a magnetic coil 2, a battery pack 3, and an electric control switch 4 are installed inside; wherein the magnetic coil 2 is electrically connected to the battery pack 3, and the battery pack 3 is electrically connected to the electric control switch 4; the electric control switch 4 is installed on the side wall of the cylindrical structure; the microneedle 1 is a structure made of magnetically conductive metal material.
[0017] Working principle: Select an animal peripheral blood sample; Lyse the animal peripheral blood with red blood cell lysis buffer, resuspend the white blood cell sample obtained by centrifugation with PBS; Add cell sorting magnetic beads (the magnetic beads are coupled with a specific antibody) to the cell sample in PBS, and bind at 37℃ for 30 minutes; Place the sample on a magnetic rack and remove the liquid; Resuspend the magnetic beads with PBS again, and add fluorescent antibodies, and bind at 37℃ for 30 minutes; Place the sample on a magnetic rack, remove the liquid, and retain about 10uL of liquid. Figure 2 As shown, spread the magnetic beads onto a glass slide and place it on a microscope stage.
[0018] See Figure 3Under the microscope, the microscope objective 6 observes the target cells on the glass slide 6 on the microscope stage structure 7. The microneedle 1 of the utility model is used to pick up the target cells with the electromagnetic microneedle. The magnetic bead carrier 8 and the carrier 9 combined with the magnetic beads can be clearly seen, and the target cells are placed in the PCR tube. Further, other measurement experiments can be performed, for example, the sample in the PCR tube can be amplified by WGA; the WGA amplification result is detected by electrophoresis, and the result is as follows: Figure 4 .
[0019] The above-mentioned embodiments only express several implementation methods of the utility model, and the description is relatively specific and detailed, but it cannot be understood as limiting the scope of the utility model patent. It should be pointed out that for ordinary technicians in this field, several modifications and improvements can be made without departing from the concept of the utility model, which all belong to the protection scope of the utility model. Therefore, the protection scope of the utility model patent shall be based on the attached claims.
Claims
1. An electromagnetic microneedle device for cell sorting, characterized in that: The invention comprises a microneedle (1) and a magnetic generating chamber; the microneedle (1) is fixedly mounted at the front end of the magnetic generating chamber; the magnetic generating chamber is a cylindrical structure, and a magnetic coil (2), a battery pack (3), and an electric control switch (4) are installed inside; the magnetic coil (2) is electrically connected to the battery pack (3), and the battery pack (3) is electrically connected to the electric control switch (4); the electric control switch (4) is mounted on the side wall of the cylindrical structure; the microneedle (1) is a structure made of magnetically conductive metal material.
2. The electromagnetic microneedle device for cell sorting according to claim 1, characterized in that: The needle tip size of the microneedle (1) is between 0.1um and 1mm.
3. The electromagnetic microneedle device for cell sorting according to claim 1, characterized in that: The battery pack (3) is an electromagnetic rod that supplies power. The electromagnetic rod generates magnetic force when the power is on, and the magnetic force disappears when the power is off.