Novel culture dish convenient for separating and culturing cells by membrane tissue block adherence method

By using threaded needles and silicone rails to fix the membrane tissue blocks in the culture dish, combined with a sealing component, the problems of difficulty in flattening and suspension of the membrane tissue blocks in the adherent culture method were solved, achieving a flat and tight adherent effect in cell culture.

CN222313190UActive Publication Date: 2025-01-07THE 924TH HOSPITAL OF THE CHINESE PEOPLES LIBERATION ARMY JOINT LOGISTICS SUPPORT FORCE
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Patent Information

Application Number
CN202420794717.7
Authority / Receiving Office
CN · China
Patent Type
Utility models(China)
Current Assignee / Owner
Filing Date
2024-04-17
Publication Date
2025-01-07
Estimated Expiration
2034-04-17

AI Technical Summary

Technical Problem

In existing technologies, membrane tissue blocks are not easy to flatten during adherent culture, are prone to wrinkling, and are difficult to adhere tightly to the bottom of the culture dish, easily becoming suspended.

Method used

A novel culture dish was designed, comprising a threaded needle, a silicone rail, and a sealing component. The threaded needle secures the tissue, the silicone rail keeps it flat, and the sealing component provides a sterile environment, ensuring that the tissue adheres tightly to the bottom of the dish.

Benefits of technology

This effectively prevents the membrane tissue blocks from wrinkling and becoming suspended during the culture process, ensuring the stability and sterile conditions of cell culture.

✦ Generated by Eureka AI based on patent content.

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Abstract

The utility model relates to the technical field of membrane tissue block culture, in particular to a novel culture dish convenient for separating and culturing cells by a membrane tissue block adherence method, which comprises a culture dish body, a fixing component, a mounting component and a sealing component, the fixing assembly comprises threaded needles and two silica gel columns, a plurality of positioning holes are formed in the culture dish body, a fixing groove is formed in the outer side of the culture dish body, the threaded needles are detachably connected with the culture dish body, the two silica gel columns are connected with the culture dish body, and the two silica gel columns are detachably connected with the culture dish body. A plurality of fixing holes are formed in the surface of each silica gel column, the mounting assembly is connected with the culture dish body, the sealing assembly is connected with the culture dish body, and the problems that when a novel culture dish for culturing primary adherent cells through a tissue block adherent method is used, membrane tissue is inconvenient to flatten, wrinkles are prone to being generated in the middle, and the culture dish is inconvenient to use are solved. The membrane tissue cannot be tightly attached to the bottom of the culture dish and is easy to suspend due to addition of a culture solution.
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Claims

1. A novel culture dish that is convenient for separating and culturing cells by the membrane tissue block adhesion method, comprising a culture dish body, characterized in that: Also includes a fixing assembly, a mounting assembly and a sealing assembly; The fixing component includes a threaded pin and a silicone bar. A plurality of positioning holes are arranged inside the culture dish body. A fixing groove is arranged on the outside of the culture dish body. There are a plurality of threaded pins, which are detachably connected to the culture dish body and respectively located in the positioning holes. There are two silicone bars, which are respectively connected to the culture dish body and located outside the threaded pin. A plurality of fixing holes are arranged on the surface of each silicone bar. The mounting component is connected to the culture dish body, and the sealing component is connected to the culture dish body.

2. A novel culture dish for facilitating separation and culture of cells by membrane tissue block adhesion method as claimed in claim 1, characterized in that: The silicone bar is 0.5 cm wide and 0.3 cm thick, and the threaded needle is 1.5 cm high.

3. A novel culture dish for facilitating separation and culture of cells by membrane tissue block adhesion method as claimed in claim 2, characterized in that: The fixing assembly also includes a needle tip and a nut. The top of each threaded needle is fixedly connected with a needle tip. There are multiple nuts, which are respectively threadedly connected to the threaded needle and are respectively located on the top of the silicone column.

4. A novel culture dish for facilitating separation and culture of cells by membrane tissue block adherence method as claimed in claim 3, characterized in that: The mounting assembly comprises a support seat and a mounting seat, wherein the support seat is fixedly connected to the culture dish body and is located at the bottom of the culture dish body, and the mounting seat is connected to the support seat and is located at the bottom of the support seat.

5. A novel culture dish for facilitating separation and culture of cells by membrane tissue block adherence method as claimed in claim 1, characterized in that: The sealing component comprises a sealing film and a sealing ring. The sealing film is connected to the culture dish body and is located outside the culture dish body. The sealing ring is connected to the sealing film and is located inside the fixing groove.