Sampling and flaking combination device
By designing a sampling and filming combination, the cell production steps in thin-layer liquid-based cytology detection technology are simplified, and the problems of high equipment costs and cumbersome operations in grassroots hospitals are solved, and work efficiency and equipment applicability are improved.
Patent Information
- Application Number
- CN202420860399.X
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-04-24
- Publication Date
- 2025-01-07
- Estimated Expiration
- 2034-04-24
AI Technical Summary
The promotion of thin-layer liquid-based cytology detection technology in grassroots hospitals is limited, and due to the cumbersome operation and high equipment cost, the production steps are inefficient.
A sampling and production combination device is designed, including a smear rack, bin and cover. Through axial chute, slot, sealing ring, filter and spike structures, it simplifies the collection, preservation and oscillation of cell samples. Mixing process to reduce operation steps.
The cell production steps are simplified, work efficiency is improved, cell samples are transferred between different containers, operational complexity and equipment cost are reduced, and it is suitable for use in grassroots hospitals.
Smart Images
Figure CN222318590U_ABST
Abstract
Description
Technical Field
[0001] The utility model relates to the technical field of pathological detection, in particular to a sampling and slicing combiner. Background Art
[0002] Thin-Cytologic Test (TCT) was born in the United States and other countries in 1991 and was first used in gynecological cytology examinations. China began research on liquid-based cytology screening for cervical cancer in 2001, which has led to the rapid development of this technology.
[0003] TCT fundamentally solves the technical problems of high false negative rate, high cell loss rate and poor smear quality in conventional exfoliated cell preparation, and has made a significant contribution to exfoliated cytological diagnosis. However, in the past, this technology required foreign slide making machines, cell preservation fluid, etc., which were expensive. Only some large hospitals in China could carry out this project, and its promotion in grassroots hospitals was relatively slow. Later, some domestic manufacturers developed semi-automatic centrifugal slide making machines and independently developed cell preservation fluid, which greatly reduced the production cost of thin layer liquid-based cells, making this technology easy to be accepted by the majority of grassroots hospitals and promoted for use. At present, in pathological detection, it is often necessary to make cell slides for microscopic observation.
[0004] Collecting cells in a pathology laboratory is a tedious task. During the sample collection stage, cell samples need to be stored in sampling bottles containing cell preservation fluid. During the sample processing stage, buffer needs to be added to the sample bottle. After shaking and mixing, the cell sample in the sampling bottle is added to the preparation chamber on the slide before centrifugal smear can be performed. The operation is cumbersome. Utility Model Content
[0005] In view of this, the utility model provides a sampling and film-making combiner, the main purpose of which is to simplify the TCT film-making steps and improve work efficiency.
[0006] In order to achieve the above purpose, the utility model mainly provides the following technical solutions:
[0007] The utility model provides a sampling and smearing joint device, which comprises: a smear rack, a storage tube and a cover body;
[0008] The upper surface of the smear rack is provided with an axial slide groove for placing the slide glass, and the opposite sides of the axial slide groove are respectively provided with a clamping groove;
[0009] The opposite sides of the lower end of the silo are respectively fixedly connected to the limit plates, the limit plates are clamped in the clamping groove, the lower end of the silo is provided with a sealing ring, the sealing ring is attached to the slide, a filter screen and spikes are arranged in the silo, the spikes are located above the filter screen, and the spikes extend out of the upper end of the silo;
[0010] The cover body is threadedly connected to the upper end of the silo, and the inner wall of the cover body is fixedly connected to the edge of the diaphragm to form a closed space between the diaphragm and the top wall of the cover body, and the closed space is filled with a buffer solution.
[0011] The purpose of the utility model and the solution to its technical problems can also be further achieved by adopting the following technical measures.
[0012] Optionally, the spike is eccentrically disposed in the silo.
[0013] Optionally, a limiting ring is further included, the outer edges of which are respectively fixedly connected to the opposite sides of the axial slide groove, so as to form the slot on the opposite sides of the axial slide groove, and arc-shaped notches are respectively provided on the opposite sides of the inner edge of the limiting ring, and the contour of the arc-shaped notch matches the contour of the limiting plate.
[0014] Optionally, the lower axial side of the silo is fixedly connected to the inner edge of the baffle ring, the outer diameter of the baffle ring matches the inner diameter of the limiting ring, and the two limiting plates are respectively fixedly connected to the opposite sides of the outer edge of the baffle ring.
[0015] Optionally, the two arc-shaped notches are arranged along the axial direction of the axial sliding groove.
[0016] Optionally, the upper end of the silo is provided with an external thread, and the cover body is provided with an internal thread, the external thread includes adjacent upper external threads and lower external threads, the internal thread is screwed into the upper external thread to create a gap between the spike and the diaphragm, and the internal thread is screwed into the lower external thread to allow the spike to cut through the diaphragm.
[0017] Optionally, one end of the axial slide groove is open, and the other end of the axial slide groove is closed.
[0018] By means of the above technical solution, the utility model has at least the following advantages:
[0019] Before using this slide making jointer, the barrel needs to be rotated forward so that the limit piece on the opposite side of the lower end of the barrel slides horizontally into the slot, so that the sealing ring is close to the slide, so that the position of the barrel, slide and smear rack is fixed to avoid leakage from the lower end of the barrel.
[0020] On the basis of the above, when sampling in the hospital, the operator adds cell preservation fluid into the silo, then immerses the collected cell sample in the cell preservation fluid, completes the operation of fixing the cells with the preservation fluid, and then screws the cover to the first depth at the upper end of the silo to form a sealed space inside the silo, but the spikes do not contact the diaphragm.
[0021] When the operator moves the preparation joint device to the testing laboratory and needs to mix and oscillate the specimen solution, the cover body is further screwed on to the second depth at the upper end of the storage tube, the sharp thorns cut the diaphragm, and the buffer solution in the enclosed space of the cover body leaks into the storage tube. The buffer solution and the cell preservation solution are mixed, and the oscillation mixing operation can be performed.
[0022] After the mucus is broken up and the specimen is mixed, an even number of slide-making jointers are directly placed in a centrifuge (slide-making machine) for centrifugation. After centrifugation, the supernatant is poured off, and then the specimen is shaken and mixed again to make the cell sediment into a uniform cell suspension, which is then placed in a centrifuge (slide-making machine) for centrifugation.
[0023] After completing the above work, take the slide making unit out of the centrifuge (slide making machine), rotate the bin in the opposite direction to disengage the limit plate from the slot, and disengage the sealing ring at the lower end of the bin from the slide. After removing the slide from the axial slide slot, dry the slide and then stain it. BRIEF DESCRIPTION OF THE DRAWINGS
[0024] Figure 1 A stereoscopic diagram of a first sampling and film-making joint device provided by an embodiment of the utility model from a first viewing angle;
[0025] Figure 2 A stereoscopic diagram of a second viewing angle of the first sampling and film-making joint device provided by an embodiment of the utility model;
[0026] Figure 3 A front view of a first sampling and film-making joint device provided in an embodiment of the utility model;
[0027] Figure 4 A side view of a first sampling and film-making joint device provided by an embodiment of the utility model;
[0028] Figure 5 A stereoscopic diagram of a second sampling and film-making joint device provided by an embodiment of the utility model from a first viewing angle;
[0029] Figure 6 A stereoscopic diagram of a second sampling and film-making joint device provided by an embodiment of the utility model from a second viewing angle;
[0030] Figure 7 A side view of a second sampling and film-making joint device provided by an embodiment of the utility model;
[0031] Figure 8 A front view of a second sampling and film-making combiner provided in an embodiment of the utility model.
[0032] The figure marks in the drawings of the specification include: smear rack 1, storage tube 2, cover body 3, axial slide groove 4, slide glass 5, card slot 6, limit plate 7, filter screen 8, spike 9, diaphragm 11, limit ring 12, baffle ring 13, arc notch 14, external thread 15, internal thread 16. DETAILED DESCRIPTION
[0033] In order to further explain the technical means and effects adopted by the utility model to achieve the predetermined utility model purpose, the specific implementation methods, structures, features and effects of the utility model application are described in detail below in conjunction with the accompanying drawings and preferred embodiments. In the following description, different "one embodiment" or "embodiment" does not necessarily refer to the same embodiment. In addition, specific features, structures, or characteristics in one or more embodiments may be combined in any suitable form.
[0034] The utility model is further described in detail below in conjunction with the accompanying drawings and embodiments.
[0035] An embodiment of the utility model provides a sampling and smearing joint device, which comprises: a smear rack 1, a storage tube 2 and a cover body 3;
[0036] The upper surface of the smear rack 1 is provided with an axial slide groove 4 for placing a glass slide 5, and opposite sides of the axial slide groove 4 are respectively provided with a clamping groove 6;
[0037] The opposite sides of the lower end of the silo 2 are respectively fixedly connected to the limit plates 7, the limit plates 7 are clamped in the clamping groove 6, the lower end of the silo 2 is provided with a sealing ring, the sealing ring is attached to the slide glass 5, the silo 2 is provided with a filter screen 8 and a spike 9, the spike 9 is located above the filter screen 8, and the spike 9 extends out of the upper end of the silo 2;
[0038] The cover body 3 is threadedly connected to the upper end of the silo 2, and the inner wall of the cover body 3 is fixedly connected to the edge of the diaphragm 11 to form a closed space between the diaphragm 11 and the top wall of the cover body 3, and the closed space is filled with a buffer solution.
[0039] The working process of a sampling and film-making joint device is as follows:
[0040] Before using the present film-making combiner, the barrel 2 needs to be rotated forward so that the limit plate 7 on the opposite side of the lower end of the barrel 2 slides horizontally into the slot 6 so that the sealing ring is in close contact with the glass slide 5, so that the positions of the barrel 2, the glass slide 5 and the smear rack 1 are fixed to prevent leakage from the lower end of the barrel 2.
[0041] On the basis of the above, when sampling in the hospital, the operator adds cell preservation solution into the silo 2, and then immerses the collected cell sample into the cell preservation solution to complete the operation of fixing the cells with the preservation solution, and then screws the cover 3 to the first depth at the upper end of the silo 2 to form a sealed space inside the silo 2, but the spikes 9 do not contact the diaphragm 11.
[0042] When the operator moves the preparation joint device to the testing laboratory and needs to mix and oscillate the specimen solution, the cover body 3 is further screwed to the second depth at the upper end of the storage tube 2, the spike 9 cuts through the diaphragm 11, and the buffer solution in the enclosed space of the cover body 3 leaks into the storage tube 2. The buffer solution and the cell preservation solution are mixed and the oscillation mixing operation can be performed.
[0043] After the mucus is broken up and the specimen is mixed, an even number of slide-making jointers are directly placed in a centrifuge (slide-making machine) for centrifugation. After centrifugation, the supernatant is poured off, and then the specimen is shaken and mixed again to make the cell sediment into a uniform cell suspension, which is then placed in a centrifuge (slide-making machine) for centrifugation.
[0044] After completing the above work, take the slide making unit out of the centrifuge (slide making machine), rotate the bin 2 in the opposite direction, so that the limit plate 7 is separated from the slot 6, and the sealing ring at the lower end of the bin 2 is separated from the slide 5. After the slide 5 is taken out of the axial slide groove 4, the slide 5 is dried and then stained.
[0045] In the technical solution of the utility model, the bin 2 is always used from the sampling step to the drying step, and there is no need to transfer the cell samples between different containers, which simplifies the TCT preparation steps and improves work efficiency.
[0046] Specifically, the sealing ring is made of elastic medical plastic material. When the limiting piece 7 is slidably inserted into the card slot 6 , the sealing ring is squeezed by the glass slide 5 to seal the gap between the lower end of the silo 2 and the glass slide 5 .
[0047] Specifically, the diaphragm 11 is made of aluminum foil, which has stable chemical properties.
[0048] Specifically, the lower end of the spike 9 is fixedly connected to the inner wall of the silo 2 , and the upper end of the spike 9 extends out of the upper port of the silo 2 .
[0049] In a specific embodiment, the spike 9 is eccentrically arranged in the silo 2 .
[0050] Specifically, if the spike 9 coincides with the central axis of the silo 2 and the central axis of the cover 3 , the spike 9 can only pierce a point-shaped through hole on the diaphragm 11 .
[0051] However, in this embodiment, when the cover 3 is screwed from the first depth to the second depth, the spike 9 cuts a circular opening on the lower surface of the diaphragm 11 to increase the mixing speed of the buffer solution and the cell preservation solution.
[0052] In a specific embodiment, it also includes a limit ring 12, the outer edges of which are fixedly connected to the opposite sides of the axial slide groove 4, respectively, for forming the slot 6 on the opposite sides of the axial slide groove 4, and the opposite sides of the inner edge of the limit ring 12 are respectively provided with arc-shaped notches 14, and the contour of the arc-shaped notch 14 is consistent with the contour of the limit plate 7.
[0053] In this embodiment, specifically, when disassembling the silo 2, the operator rotates the silo 2 in the opposite direction. Due to the constraint of the limiting ring 12, the silo 2 can only rotate within the limiting ring 12 with the central axis of the silo 2 as the center. The sealing ring at the lower end of the silo 2 will not touch the area of the slide 5 coated with cells, thereby avoiding the loss of the specimen.
[0054] In a specific embodiment, the lower axial side of the silo 2 is fixedly connected to the inner edge of the baffle ring 13, the outer diameter of the baffle ring 13 matches the inner diameter of the limiting ring 12, and the two limiting plates 7 are respectively fixedly connected to the opposite sides of the outer edge of the baffle ring.
[0055] Specifically, the inner edge contour of the slot 6 matches the contour of the limiting piece 7, and both are arc-shaped. When the slide glass 5 slides in the axial slide groove 4, the slide glass 5 cannot cover the inner area of the slot 6. If the limiting piece 7 is directly fixedly connected to the lower end axial side of the silo 2, when the slide making jointer is used, the distance between the inner edge of the silo 2 and the edge of the slide glass 5 will be smaller.
[0056] In this embodiment, the outer diameter of the baffle ring is consistent with the inner diameter of the limiting ring 12, and the distance between the inner edge of the silo 2 and the edge of the slide 5 at least increases the radial width of the baffle ring, reducing the possibility of liquid inside the silo 2 leaking from the edge of the slide 5.
[0057] In a specific implementation, the two arc-shaped notches 14 are arranged along the axial direction of the axial sliding groove 4 .
[0058] In this embodiment, specifically, the two arc-shaped notches 14 are arranged along the axial direction of the axial slide groove 4, and the limiting piece 7 rotates from the slot 6 to the arc-shaped notch 14. The silo 2 needs to rotate 90° to seal the internal space of the silo 2 to the greatest extent.
[0059] In a specific embodiment, the upper end of the silo 2 is provided with an external thread 15, and the cover body 3 is provided with an internal thread 16, the external thread 15 includes adjacent upper external threads 15 and lower external threads 15, the internal thread 16 is screwed into the upper external thread 15, and is used to create a gap between the spike 9 and the diaphragm 11, and the internal thread 16 is screwed into the lower external thread 15, and is used to allow the spike 9 to scratch the diaphragm 11.
[0060] In this embodiment, specifically, a first marking line is provided between the upper external thread 15 and the lower external thread 15 , the cover body 3 is made of transparent plastic material, and a second marking line is provided at the lower end of the internal thread 16 .
[0061] When the operator takes samples in the hospital, he screws the cover 3 to the first depth at the upper end of the silo 2 (i.e., when the first mark line and the second mark line coincide, he stops screwing the cover 3 downward); when oscillation and mixing are required, he continues to screw the cover 3 downward to the second depth (the second mark line passes over the first mark line), the spikes 9 cut through the diaphragm 11, and the buffer enters the silo 2 to mix with the cell preservation solution.
[0062] In a specific implementation, one end of the axial sliding groove 4 is open, and the other end of the axial sliding groove 4 is closed.
[0063] In this embodiment, specifically, the operator can insert or pull out the slide 5 from the open end of the axial slide 4, but cannot pull it out from the closed end of the axial slide 4. In this way, when using a centrifuge (slide making machine) for centrifugation, the closed end of the axial slide 4 is placed outward to ensure that the slide 5 cannot slide out of the axial slide 4 due to centrifugal force.
[0064] The above is only a specific implementation of the utility model, but the protection scope of the utility model is not limited thereto. Any technician familiar with the technical field can easily think of changes or substitutions within the technical scope disclosed by the utility model, which should be included in the protection scope of the utility model. Therefore, the protection scope of the utility model should be based on the protection scope of the claims.
Claims
1. A sampling and film-making combined device, characterized in that: include: A smear rack, wherein the upper surface of the smear rack is provided with an axial slide groove for placing a glass slide, and opposite sides of the axial slide groove are respectively provided with a clamping groove; A silo, wherein opposite sides of the lower end of the silo are respectively fixedly connected to the limit plates, the limit plates are clamped in the clamping groove, a sealing ring is provided at the lower end of the silo, the sealing ring is fitted to the slide, a filter screen and spikes are provided in the silo, the spikes are located above the filter screen, and the spikes extend out of the upper end of the silo; The cover body is threadedly connected to the upper end of the silo, and the inner wall of the cover body is fixedly connected to the edge of the diaphragm to form a closed space between the diaphragm and the top wall of the cover body, and the closed space is filled with a buffer solution.
2. The sampling and film-making joint device according to claim 1, characterized in that: The spike is eccentrically arranged in the silo.
3. The sampling and film-making joint device according to claim 1, characterized in that: It also includes a limiting ring, the outer edges of which are respectively fixedly connected to the opposite sides of the axial slide groove, so as to form the slot on the opposite sides of the axial slide groove, and the opposite sides of the inner edge of the limiting ring are respectively provided with arc-shaped notches, and the contour of the arc-shaped notches matches the contour of the limiting plate.
4. The sampling and film-making joint device according to claim 3, characterized in that: The lower end axial side of the silo is fixedly connected to the inner edge of the baffle ring, the outer diameter of the baffle ring matches the inner diameter of the limiting ring, and the two limiting plates are respectively fixedly connected to the opposite sides of the outer edge of the baffle ring.
5. The sampling and film-making joint device according to claim 3, characterized in that: The two arc-shaped notches are arranged along the axial direction of the axial sliding groove.
6. The sampling and film-making joint device according to any one of claims 1 to 5, characterized in that: The upper end of the silo is provided with an external thread, and the cover body is provided with an internal thread. The external thread includes adjacent upper external threads and lower external threads. The internal thread is screwed into the upper external thread to create a gap between the spike and the diaphragm. The internal thread is screwed into the lower external thread to allow the spike to cut through the diaphragm.
7. The sampling and film-making joint device according to any one of claims 1 to 5, characterized in that: One end of the axial sliding groove is open, and the other end of the axial sliding groove is closed.