Device for uniformly mixing Escherichia coli resuspension and alkali lysis solution

Abstract

The utility model belongs to the technical field of mixing devices, and particularly relates to a device for uniformly mixing an escherichia coli resuspension solution and an alkali lysis solution, which comprises a processing tank and a base fixedly connected with the processing tank, and is characterized in that a liquid inlet pipe is connected onto the processing tank, a servo motor is fixedly mounted at the axis of the bottom of the processing tank, and a liquid outlet pipe is connected onto the base. An output shaft is fixedly connected to the output end of the servo motor, a flow guide cap is fixedly connected to the top of the output shaft, a flow guide groove is formed in the flow guide cap, stirring blades are fixedly connected to the periphery of the output shaft, and one end of the processing tank communicates with a fixed material pouring shell through a notch. Through rotation of the stirring blades and guidance of the flow guide cap, escherichia coli resuspension liquid and alkali lysis liquid can be effectively and uniformly mixed, accurate control over the mixing process and the discharging process can be achieved through the use of the servo motor and the electric push rod, the structural design is relatively simple, operation and maintenance are easy, liquid mixing and treating efficiency can be improved, and the production cost is reduced. And time and resources are saved.

Description

Technical Field

[0001] The utility model relates to the technical field of mixing devices, in particular to a device for mixing Escherichia coli resuspension liquid and alkaline lysis liquid. Background Art

[0002] The alkaline lysis method is commonly used to extract plasmid DNA. It is the most widely used method for preparing plasmid DNA. Its basic operation method is: first centrifuge the E. coli liquid and then resuspend it. The cell wall and cell membrane of the E. coli resuspended liquid are destroyed under alkaline conditions (pH12.5), and the intracellular substances are released. Under high alkaline conditions, the double helix structure of the linear chromosome DNA is untied and denatured. Although the hydrogen bonds of the plasmid DNA are broken, the two complementary chains are entangled with each other and tightly bound together. When potassium acetate is added to restore to neutrality, the chromosome DNA molecules are difficult to renature, while the plasmid DNA molecules renature quickly. During centrifugation, the chromosome DNA is precipitated together with the cell fragments, while the plasmid DNA remains in the supernatant. After precipitation with isopropanol or ethanol, washing can obtain relatively pure plasmid DNA.

[0003] During the alkaline lysis process, stirring is required to mix the components evenly, which is often done by manual stirring. However, due to uncontrollable factors such as the speed and strength of manual stirring, insufficient mixing may occur, which may easily lead to DNA damage and low extraction efficiency. The market needs a device that can control the precise mixing of bacterial resuspension and lysis solution.

[0004] Therefore, we proposed a device for mixing Escherichia coli resuspension and alkaline lysis solution to solve the above problems. Utility Model Content

[0005] 1. Technical issues to be solved

[0006] In view of the deficiencies of the prior art, the utility model provides a device for mixing Escherichia coli resuspension and alkaline lysis solution, which solves the problems raised in the above-mentioned background technology.

[0007] (II) Technical solution

[0008] In order to achieve the above-mentioned purpose, the utility model specifically adopts the following technical solutions:

[0009] A device for mixing Escherichia coli resuspension and alkaline lysis solution, comprising a processing tank and a base fixedly connected to the processing tank, characterized in that: the processing tank is connected to a liquid inlet pipe, a servo motor is fixedly installed at the bottom axis of the processing tank, the output end of the servo motor is fixedly connected to an output shaft, the top of the output shaft is fixedly connected to a guide cap, the guide cap is provided with a guide groove, the output shaft is fixedly connected with stirring blades around, one end of the processing tank is connected to a fixed pouring shell through a notch, one end of the pouring shell is fixedly connected to an electric push rod, one end of the electric push rod is fixedly connected to a mounting frame, one end of the mounting frame is connected to a sealing door, the sealing door has the same shape as the notch and is used to seal the notch.

[0010] Furthermore, the two liquid inlet pipes are divided into a group, one group of liquid inlet pipes are horizontally parallel, and the other group of liquid inlet pipes are arranged at symmetrical angles in the vertical direction.

[0011] Furthermore, a liquid spray device for resuspending bacterial cell buffer and an alkaline solution liquid spray device are installed inside the liquid inlet pipe.

[0012] Furthermore, the stirring blades are divided into four, one is a scraper plate, one is a flat plate, one is a push plate, and one is a guide plate.

[0013] Furthermore, the scraper plate contacts the inner wall of the processing tank, the flat plate is a 45° inclined plate, the push plate is a 15° inclined plate, the interface of the guide plate is an arc shape, and the guide plate contacts the bottom of the processing tank.

[0014] (III) Beneficial effects

[0015] Compared with the prior art, the utility model provides a device for mixing Escherichia coli resuspension and alkaline lysis solution, which has the following beneficial effects:

[0016] The utility model can effectively mix the Escherichia coli resuspension and the alkaline lysis solution through the rotation of the stirring blades and the guidance of the guide cap. The use of the servo motor and the electric push rod can realize accurate control of the mixing process and the discharging process. The structural design is relatively simple and easy to operate and maintain, which helps to improve the efficiency of mixing and processing liquids and save time and resources. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] Figure 1 It is a schematic diagram of the structure of the utility model;

[0018] Figure 2 This is a side view of the structure of the utility model;

[0019] Figure 3 This is a schematic diagram of the structure of the servo motor of the utility model;

[0020] Figure 4 It is a schematic diagram of the inverted shell structure of the utility model.

[0021] In the figure: 1. Processing tank; 2. Base; 3. Liquid inlet pipe; 4. Servo motor; 5. Output shaft; 6. Guide cap; 7. Guide groove; 8. Stirring blade; 9. Notch; 10. Reverse shell; 11. Electric push rod; 12. Mounting frame; 13. Sealing door. DETAILED DESCRIPTION

[0022] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.

[0023] Example

[0024] like Figure 1-4 As shown, an embodiment of the utility model proposes a device for mixing Escherichia coli resuspension and alkaline lysis solution, comprising a processing tank 1 and a base 2 fixedly connected to the processing tank 1, the processing tank 1 is connected to a liquid inlet pipe 3, a servo motor 4 is fixedly installed at the bottom axis of the processing tank 1, the output end of the servo motor 4 is fixedly connected to an output shaft 5, the top of the output shaft 5 is fixedly connected to a guide cap 6, a guide groove 7 is provided on the guide cap 6, and stirring blades 8 are fixedly connected to the four sides of the output shaft 5, one end of the processing tank 1 is connected to a fixed pouring shell 10 through a notch 9, one end of the pouring shell 10 is fixedly connected to an electric push rod 11, one end of the electric push rod 11 is fixedly connected to a mounting frame 12, one end of the mounting frame 12 is connected to a sealing door 13, and the sealing door 13 has the same shape as the notch 9 and is used to seal the notch 9;

[0025] The liquid inlet pipe 3 is used to input atomized liquids such as E. coli resuspension liquid and alkaline lysis liquid;

[0026] The servo motor 4 drives the guide cap 6 and the stirring blade 8 to rotate through the output shaft 5. The guide groove 7 on the guide cap 6 can guide the flow of the liquid and promote mixing;

[0027] The rotation of the stirring blade 8 can further mix the liquid;

[0028] The liquids are mixed in the processing tank 1;

[0029] When the mixed liquid needs to be poured out, the electric push rod 11 pushes the mounting frame 12 to make the sealing door 13 open the gap 9;

[0030] The mixed liquid can then be discharged through the pouring shell 10.

[0031] The entire device is driven by a motor, mixed by a stirring blade 8 and controlled by a sealing door 13, thereby achieving effective mixing and discharge of the E. coli resuspension and the alkaline lysis solution.

[0032] The design of this device can improve the mixing efficiency and effect, ensuring that the liquids are mixed evenly.

[0033] like Figure 2 As shown, in some embodiments, the two liquid inlet pipes 3 are divided into a group, one group of liquid inlet pipes 3 is horizontally parallel, and the other group of liquid inlet pipes 3 is set at a symmetrical angle in the vertical direction. The bacterial heavy suspension and the alkali solution are respectively connected to the spray device through pipelines and pumps; after the liquid collision is completed, it is collected through the processing tank 1 for further mixing.

[0034] like Figure 2 As shown, in some embodiments, the liquid spray device for resuspending the bacterial cell buffer and the alkali liquid spray device are installed inside the liquid inlet pipe 3. This part is installed in the prior art. At the same time, the liquid spray device for resuspending the bacterial cell buffer is used to spray the resuspended bacterial cell buffer, and the alkali liquid spray device is used to spray the alkali liquid. The bacterial cell resuspended liquid and the alkali liquid are atomized and sprayed into the processing tank 1 to perform collision mixing of the atomized liquids.

[0035] like Figure 3 As shown, in some embodiments, the stirring blade 8 is divided into four pieces, one is a scraper plate, one is a flat plate, one is a push plate, and one is a guide plate. The four blades can be designed at different heights.

[0036] Scraper: used to scrape the inner wall of the processing tank 1 to prevent material from adhering or promote mixing;

[0037] Flat plate: The shape is relatively flat and can play a certain mixing role during the stirring process;

[0038] Push plate: It is assumed that it has the function of pushing the material and helps the movement of the material in the tank;

[0039] Guide plate: Its arc-shaped interface is conducive to guiding the flow of materials.

[0040] like Figure 3 As shown, in some embodiments, the scraper plate contacts the inner wall of the processing tank 1, the flat plate is a plate inclined at 45°, the push plate is a plate inclined at 15°, the interface of the guide plate is an arc shape, and the guide plate contacts the bottom of the processing tank 1;

[0041] The scraper plate can increase the stability of the structure and remove the liquid from the inner arm;

[0042] The inclination angles of the flat plate and push plate help to achieve a specific flow-guiding action for the fluid;

[0043] The arc-shaped interface of the guide plate can better guide the flow of fluid or material.

[0044] Finally, it should be noted that the above description is only a preferred embodiment of the utility model and is not intended to limit the utility model. Although the utility model is described in detail with reference to the above embodiments, those skilled in the art can still modify the technical solutions recorded in the above embodiments or replace some of the technical features therein by equivalents. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the utility model shall be included in the protection scope of the utility model.

Claims

1. A device for mixing a resuspended Escherichia coli solution with an alkaline lysis solution, comprising a processing tank (1) and a base (2) fixedly connected to the processing tank (1), characterized in that: The processing tank (1) is connected to a liquid inlet pipe (3), a servo motor (4) is fixedly installed at the bottom axis of the processing tank (1), an output end of the servo motor (4) is fixedly connected to an output shaft (5), a flow guide cap (6) is fixedly connected to the top of the output shaft (5), a flow guide groove (7) is provided on the flow guide cap (6), stirring blades (8) are fixedly connected to the four sides of the output shaft (5), one end of the processing tank (1) is connected to a fixed pouring shell (10) through a notch (9), one end of the pouring shell (10) is fixedly connected to an electric push rod (11), one end of the electric push rod (11) is fixedly connected to a mounting frame (12), one end of the mounting frame (12) is connected to a sealing door (13), the sealing door (13) has the same shape as the notch (9) and is used to seal the notch (9).

2. The device for mixing Escherichia coli resuspension and alkaline lysis solution according to claim 1, characterized in that: The two liquid inlet pipes (3) are divided into a group, one group of liquid inlet pipes (3) are horizontally parallel, and the other group of liquid inlet pipes (3) are arranged at symmetrical angles in the vertical direction.

3. The device for mixing Escherichia coli resuspension and alkaline lysis solution according to claim 2, characterized in that: A liquid spray device for resuspending bacterial cell buffer and an alkali liquid spray device are installed inside the liquid inlet pipe (3).

4. The device for mixing Escherichia coli resuspension and alkaline lysis solution according to claim 1, characterized in that: The stirring blade (8) is divided into four parts, one is a scraping plate, one is a flat plate, one is a pushing plate, and one is a guide plate.

5. The device for mixing Escherichia coli resuspension and alkaline lysis solution according to claim 4, characterized in that: The scraper plate contacts the inner wall of the processing tank (1), the flat plate is a plate inclined at 45°, the push plate is a plate inclined at 15°, the interface of the guide plate is arc-shaped, and the guide plate contacts the bottom of the processing tank (1).

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