Black rice protein peptide separation and purification device

Abstract

The utility model relates to the technical field of protein peptide separation and purification, and discloses a black rice protein peptide separation and purification device which comprises a base and a top cover, a shell is arranged on the periphery of the upper portion of the base, an anti-falling frame is arranged on the periphery of the outer side of the top cover, and a driving chassis and a supporting rotating shaft are arranged in the middle of the inner side of the base. A limiting ring is arranged at the top of the supporting rotating shaft, a separation column is arranged on the inner side of the limiting ring, positioning grooves are formed in the two sides of the top of the limiting ring, a supporting ring is arranged on the outer side of the upper portion of the separation column, and positioning blocks are arranged on the two sides of the bottom of the supporting ring. The separation and purification device for the black rice protein peptide is simple in structure and easy to operate, the labor cost is saved, and the consumption of expensive reagents is reduced and the separation and purification cost is reduced by utilizing a ligand of the black rice protein peptide or a specific adsorbent of an elution device for other specific reagents.

Description

Technical Field

[0001] The utility model relates to the technical field of protein peptide separation and purification, in particular to a device for separating and purifying umbelliferyl protein peptide. Background Art

[0002] Wumi protein peptide is a biomacromolecule with multiple biological activities and is widely used in medicine, food, cosmetics and other fields. However, the content of Wumi protein peptide in natural resources is low and is interfered by other impurities, so it needs to be separated and purified.

[0003] Traditional separation and purification methods such as gel filtration, ion exchange, affinity chromatography, etc. have problems such as complex operation, high cost and low efficiency. Utility Model Content

[0004] The utility model aims to provide a device for separating and purifying umami protein peptides, which can solve the problems of complex operation, high cost and low efficiency, and has the characteristics of simple operation, low cost and high efficiency.

[0005] To achieve the above-mentioned purpose, a device for separating and purifying umami protein peptide is provided, comprising a base and a top cover, a shell is arranged around the top of the base, an anti-slip frame is arranged around the outer side of the top cover, a driving chassis and a supporting shaft are respectively arranged in the middle of the inner side of the base, a limiting ring is arranged on the top of the supporting shaft, a separation column is arranged on the inner side of the limiting ring, positioning grooves are arranged on both sides of the top of the limiting ring, a supporting ring is arranged on the outer side above the separation column, positioning blocks are arranged on both sides of the bottom of the supporting ring, a control panel is arranged on the front side of the outside of the base, an granule tube is arranged on the inner side of the middle part of the top cover, an elution device is arranged on the top of the granule tube, and the elution device is arranged on the upper surface of the top cover.

[0006] According to the device for separating and purifying umami protein peptides, the top cover is made of transparent glass, the upper part of the base is fixedly contacted and connected to the shell, the outer part of the top cover is fixedly connected to the anti-slip frame, and the top cover is connected to the space above the shell through the anti-slip frame and the upper outer surface of the shell to form a supporting contact effect.

[0007] According to the device for separating and purifying umami protein peptides, the top of the driving chassis is connected to the supporting shaft in a limiting contact manner, the driving chassis and the supporting shaft form a T-shaped structure, and the driving chassis and the supporting shaft are connected in a snap-fitting contact manner with the middle portion of the inner side of the base.

[0008] According to the device for separating and purifying umami protein peptides, the control panel is fixedly contacted with the front side of the outside of the base, the driving chassis is connected to the control panel for signal transmission, and the supporting shaft forms a rotation effect above the driving chassis through the signal transmission formed by the driving chassis and the control panel.

[0009] According to the device for separating and purifying umami protein peptides, the limiting ring is fixedly contacted and connected with the top of the supporting shaft, the separation column usually uses a silica gel column with a C18 alkyl chain of a chemically bonded phase, and the separation column is limitedly contacted and connected with the inside of the limiting ring.

[0010] According to the device for separating and purifying umami protein peptides, the support ring is fixedly connected to the outer side above the separation column, both sides of the top of the limiting ring are recessedly connected to the positioning groove, and both sides of the bottom of the support ring are fixedly contacted with the positioning block. The separation column forms a snap-fitting positioning effect with the inside of the limiting ring through the snap-fitting connection between the positioning block at the bottom of the support ring and the positioning groove.

[0011] According to the device for separating and purifying the umami protein peptide, the elution device is filled with the ligand of the umami protein peptide or other specific reagents, and the elution device is contact-connected with the surface support above the top cover.

[0012] According to the device for separating and purifying umami protein peptides, the granule tube is pressure-connected and contacted with the elution device through a limiting contact connection on the inner side of the middle part of the top cover, and the bottom end of the granule tube forms a supporting effect with the space above the separation column through the inner side of the middle part of the top cover.

[0013] The above scheme has the beneficial effects: through the provision of the base, shell, top cover and anti-detachment frame, the structure of the umami protein peptide separation and purification device is simple in structure, easy to operate, and saves labor costs. The specific adsorbent of the elution device containing the umami protein peptide ligand or other specific reagents is utilized to reduce the consumption of expensive reagents and reduce the cost of separation and purification.

[0014] Additional aspects and advantages of the present invention will be given in part in the following description, and in part will become apparent from the following description, or will be learned through the practice of the present invention. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] Figure 1 This is a schematic diagram of the structure of a device for separating and purifying umami protein peptides according to the utility model;

[0016] Figure 2 This is a structural cross-sectional view of the top cover of a device for separating and purifying umami protein peptides according to the utility model;

[0017] Figure 3 This is a structural cross-sectional view of the shell of a device for separating and purifying umami protein peptides according to the utility model;

[0018] Figure 4 It is a front perspective view of a device for separating and purifying umami protein peptides according to the utility model.

[0019] Legend:

[0020] 1. Base; 2. Shell; 3. Top cover; 4. Anti-slip frame; 5. Elution device; 6. Control panel; 7. Fluid tube; 8. Driving chassis; 9. Support shaft; 10. Limiting ring; 11. Separation column; 12. Support ring; 13. Positioning groove; 14. Positioning block. DETAILED DESCRIPTION

[0021] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.

[0022] Reference Figure 1-4 The utility model embodiment is a device for separating and purifying umami protein peptide, which includes a base 1 and a top cover 3. A shell body 2 is arranged around the top of the base 1, an anti-slip frame 4 is arranged around the outer side of the top cover 3, a driving chassis 8 and a supporting shaft 9 are respectively arranged in the middle of the inner side of the base 1, a limiting ring 10 is arranged on the top of the supporting shaft 9, a separation column 11 is arranged on the inner side of the limiting ring 10, and positioning grooves 13 are arranged on both sides of the top of the limiting ring 10, a support ring 12 is arranged on the outer side above the separation column 11, and positioning blocks 14 are arranged on both sides of the bottom of the support ring 12, a control panel 6 is arranged on the front side of the outside of the base 1, an ion tube 7 is arranged on the inner side of the middle part of the top cover 3, an elution device 5 is arranged on the top of the ion tube 7, and the elution device 5 is arranged on the upper surface of the top cover 3.

[0023] The top cover 3 is made of transparent glass, and the upper part of the base 1 is fixedly contacted with the shell 2, and the outer part of the top cover 3 is fixedly connected with the anti-slip frame 4. The top cover 3 is connected to the upper outer surface of the shell 2 through the anti-slip frame 4, and the top cover 3 is connected to the upper space of the shell 2 to form a supporting contact effect. The transparent glass top cover 3 is arranged to facilitate observation of the state of the high-speed rotation of the umami protein peptide solution inside the shell 2. The upper part of the driving chassis 8 is contacted with the support shaft 9 to limit the contact, and the driving chassis 8 and the support shaft 9 form a T-shaped structure. The driving chassis 8 and the support shaft 9 are contacted and connected with the middle part of the inner side of the base 1. The support shaft 9 is arranged to provide a rotating base for the rotation of the limiting ring 10, so as to facilitate the flow rate of the umami protein peptide solution. The control panel 6 is fixedly contacted with the outer front side of the base 1, and the driving chassis 8 is connected to the control panel 6 for signal transmission control. The support shaft 9 forms a rotation effect with the upper part of the driving chassis 8 through the signal transmission formed by the driving chassis 8 and the control panel 6. The limiting ring 10 is fixedly contacted with the top of the support shaft 9, and the separation column 11 is usually made of chemical The separation column 11 is connected to the inner limit position of the limiting ring 10 by setting the separation column 11 of the silica gel column of the C18 alkyl chain of the chemically bonded phase, which is suitable for the separation of hydrophobic proteins and peptides. The support ring 12 is fixedly connected to the outer side above the separation column 11, and both sides of the top of the limiting ring 10 are connected to the positioning groove 13 by a recessed engagement, and both sides of the bottom of the support ring 12 are fixedly connected to the positioning block 14. The separation column 11 is connected by the engagement between the positioning block 14 at the bottom of the support ring 12 and the positioning groove 13. A snap-fitting positioning effect is formed with the inside of the limiting ring 10. The positioning groove 13 and the positioning block 14 are set to prevent the limiting ring 10 from rotating at a fast speed, which reduces the rotation effect of the separation column 11. The elution device 5 is filled with the ligand of the umami protein peptide or other specific reagents. The elution device 5 is supported and contacted with the upper surface of the top cover 3. The granule tube 7 is pressure-connected and contacted with the elution device 5 through the limiting contact connection on the inner side of the middle part of the top cover 3. The bottom end of the granule tube 7 forms a supporting effect with the space above the separation column 11 through the inner side of the middle part of the top cover 3.

[0024] Working principle: first, the top cover 3 and the anti-detachment frame 4 are separated from the top of the shell 2 to expose the separation column 11 inside the shell 2, and the umami protein peptide solution to be separated is introduced into the separation column 11, and then the top cover 3 and the anti-detachment frame 4 are in a supporting contact state with the top of the shell 2, and the control panel 6 is turned on to adjust the temperature parameters of the environment containing the umami protein peptide solution, and the umami protein peptide solution is allowed to flow in the separation column 11, and the umami protein peptide is adsorbed on the specific adsorbent on the separation column 11 and separated from other impurities, and then the elution device 5 is turned on, and the specific binding agent of the umami protein peptide is added to the inside of the separation column 11 by the granule tube 7 to elute the umami protein peptide adsorbed on the separation column 11, and later, the top cover 3 and the anti-detachment frame 4 are separated from the top of the shell 2 to facilitate the separation of the separation column 11 and the inside of the limiting ring 10, so as to achieve the purpose of collecting the umami protein peptide, and clean the separation column 11 for the next separation and purification.

[0025] Finally, it should be noted that the above is only a preferred embodiment of the present invention and is not intended to limit the present invention. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art can still modify the technical solutions described in the aforementioned embodiments or make equivalent substitutions for some of the technical features therein. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the protection scope of the present invention.

Claims

1. A device for separating and purifying umbelliferyl protein peptides, comprising a base (1) and a top cover (3), characterized in that: A shell (2) is arranged around the top of the base (1); an anti-slip frame (4) is arranged around the outer side of the top cover (3); a driving chassis (8) and a supporting shaft (9) are arranged in the middle of the inner side of the base (1); a limiting ring (10) is arranged on the top of the supporting shaft (9); a separation column (11) is arranged on the inner side of the limiting ring (10); positioning grooves (13) are arranged on both sides of the top of the limiting ring (10); a supporting ring (12) is arranged on the outer side above the separation column (11); positioning blocks (14) are arranged on both sides of the bottom of the supporting ring (12); a control panel (6) is arranged on the front side of the outer side of the base (1); an infusion tube (7) is arranged on the inner side of the middle part of the top cover (3); an elution device (5) is arranged on the top of the infusion tube (7); and the elution device (5) is arranged on the upper surface of the top cover (3).

2. A device for separating and purifying umami protein peptides according to claim 1, characterized in that: The top cover (3) is made of transparent glass, the upper periphery of the base (1) is fixedly connected to the shell (2), the outer periphery of the top cover (3) is fixedly connected to the anti-slip frame (4), and the top cover (3) is connected to the space above the shell (2) by snapping the anti-slip frame (4) and the upper outer surface of the shell (2) to form a supporting contact effect.

3. A device for separating and purifying umami protein peptides according to claim 1, characterized in that: The driving chassis (8) is connected to the supporting shaft (9) in a limited contact manner at the top, the driving chassis (8) and the supporting shaft (9) form a T-shaped structure, and the driving chassis (8) and the supporting shaft (9) are connected to the middle part of the inner side of the base (1) in a clamping contact manner.

4. A device for separating and purifying umbelliferyl protein peptide according to claim 1, characterized in that: The control panel (6) is fixedly contacted with the front side of the outside of the base (1), the driving chassis (8) is connected with the control panel (6) for signal transmission control, and the supporting shaft (9) forms a rotation effect above the driving chassis (8) through signal transmission formed by the driving chassis (8) and the control panel (6).

5. A device for separating and purifying umami protein peptides according to claim 1, characterized in that: The limiting ring (10) is fixedly contacted and connected with the top of the supporting shaft (9), and the separation column (11) usually uses a silica gel column with a C18 alkyl chain of a chemically bonded phase. The separation column (11) is internally contacted and connected with the limiting ring (10).

6. A device for separating and purifying umami protein peptides according to claim 1, characterized in that: The support ring (12) is fixedly connected to the outer side of the upper part of the separation column (11); both sides of the top of the limit ring (10) are recessed and connected with the positioning groove (13); both sides of the bottom of the support ring (12) are fixedly contacted and connected with the positioning block (14); the separation column (11) forms a snap-fitting positioning effect with the inside of the limit ring (10) through the snap-fitting connection between the positioning block (14) at the bottom of the support ring (12) and the positioning groove (13).

7. A device for separating and purifying umami protein peptides according to claim 1, characterized in that: The elution device (5) is filled with a ligand of the umami protein peptide or other specific reagents, and the elution device (5) is connected to the upper surface of the top cover (3) in a supporting contact manner.

8. A device for separating and purifying umbelliferyl protein peptide according to claim 7, characterized in that: The granule tube (7) is pressure-connected and contact-connected with the elution device (5) via a limiting contact connection on the inner side of the middle part of the top cover (3), and the bottom end of the granule tube (7) forms a supporting effect with the space above the separation column (11) via the inner side of the middle part of the top cover (3).

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