Anti-pollution culture dish for solid culture
By designing a double-circle structure of anti-pollution culture dish, the gap between the annular outer wall and the inner wall is used to limit the opening angle and cover the gap of the dish lid, the problem of microbial contamination is solved, and the effect of minimizing exogenous contamination in microbial culture is achieved.
Patent Information
- Application Number
- CN202421086562.8
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-05-19
- Publication Date
- 2025-01-14
- Estimated Expiration
- 2034-05-19
AI Technical Summary
During the microbial or cell culture process, opening and inverting the culture dish can easily lead to exogenous microbial contamination, especially spores of filamentous fungi enter through the gap between the lid and the dish body, thereby contaminating the culture medium.
A double-circle structure anti-pollution petri dish is designed, including an annular outer wall and an inner wall. The lid is placed on the annular inner wall. The gap between the annular outer wall and the inner wall limits the opening angle of the dish lid and reduces the entry of microorganisms in the air. The gap formed between the lid and the body of the dish is covered by an annular outer wall to prevent spore contamination.
It effectively reduces microbial contamination, especially the contamination of filamentous fungi, is simple to operate, low cost, and has no difference from conventional petri dishes during cleaning and sterilization.
Smart Images
Figure CN222348998U_ABST
Abstract
Description
Technical Field
[0001] The utility model relates to an anti-pollution culture dish used for solid culture. Background Art
[0002] At present, most of the plate separation and purification of microbial cells such as bacteria and fungi or the culture of animal and plant tissues use culture dishes. The common culture dishes in the laboratory are composed of a dish body and a dish cover made of glass or plastic. When the experimenter or researcher is culturing microorganisms or cells, on the one hand, since the dish cover needs to be opened when pouring the plate and inoculating, this process can easily cause microbial contamination outside the culture dish, resulting in experimental failure, especially for beginners, microbial cross contamination is more serious; on the other hand, for a very skilled experimenter or researcher, even if strict aseptic operation is performed during actual operations such as pouring the plate and inoculating on the sterile clean bench, when the plate is taken out of the sterile clean bench for conventional inverted culture, it is often found that the edges of some plates are contaminated with filamentous fungi. The only possible reason is that the spores of filamentous fungi in the air may settle into the gap between the dish cover and the dish body formed when the culture dish is inverted. Then, the spores can germinate, grow and reproduce with the help of a small amount of condensed water carrying trace culture medium components in the gap, and finally the hyphae contaminate the cultured microorganisms by extending along the inner wall. Utility Model Content
[0003] In order to solve the above technical problems, the utility model provides an anti-pollution culture dish for solid culture which has simple structure, convenient operation, low cost and can prevent contamination caused by exogenous microorganisms during microbial experimental operation.
[0004] The technical solution adopted by the utility model is:
[0005] The invention discloses a pollution-proof culture dish for solid culture, comprising a double-ring dish body and a dish cover. The double-ring dish body comprises a dish bottom, an annular outer wall and an annular inner wall; the annular outer wall and the annular inner wall are coaxially fixed on the dish bottom, and the dish cover is sleeved on the annular inner wall of the dish body.
[0006] Preferably, the annular inner wall is higher than the annular outer wall, the inner diameter of the dish cover is larger than the outer diameter of the annular inner wall, and the outer diameter of the dish cover is smaller than the inner diameter of the annular outer wall.
[0007] Preferably, the thickness of the dish cover, the annular inner wall and the annular outer wall are all 2 mm, and the gap between the annular outer wall and the annular inner wall is no more than 7 mm.
[0008] Compared with the prior art, the beneficial effects of the utility model are:
[0009] The utility model has a simple structure and low cost. When the utility model is used for microbial inoculation, if the conventional operation is followed, when the left hand holds the culture dish and the thumb is used to open the cover, the small interval between the annular outer wall and the annular inner wall limits the angle of opening the dish cover (controlled within 45 degrees), thereby minimizing the microbial cells from the air from entering the utility model to cause contamination, and the operation is convenient.
[0010] When the microorganism solid is inverted for culture, the annular outer wall can cover a circle of space formed between the annular inner wall and the dish cover, thereby effectively preventing the contamination of exogenous spore-forming filamentous fungi.
[0011] The utility model can be cleaned just like a conventional culture dish, and is not difficult to clean; it can be sterilized just like a conventional culture dish, and can be sterilized by wet heat high pressure sterilization and dry heat high temperature sterilization; it can be operated just like a conventional culture dish, and does not affect the operating habits. BRIEF DESCRIPTION OF THE DRAWINGS
[0012] Figure 1 It is a structural schematic diagram of the utility model when it is opened.
[0013] Figure 2 It is a schematic diagram of the structure of the utility model when it is opened upside down. DETAILED DESCRIPTION
[0014] The utility model is described in detail below in conjunction with the accompanying drawings.
[0015] like Figure 1-2 As shown, the utility model comprises a dish cover 1 and a double-circle dish body 2, wherein the double-circle dish body 2 comprises a dish bottom 21, an annular outer wall 22 and an annular inner wall 23; the annular outer wall 22 and the annular inner wall 23 are coaxially fixed on the dish bottom 21, and the annular inner wall 23 is higher than the annular outer wall 22. The dish bottom 21 is circular and coaxial with the annular outer wall 22, and the diameter of the dish bottom 21 is the same as the outer diameter of the annular outer wall 22. The inner diameter of the dish cover 1 is larger than the outer diameter of the annular inner wall 23, and the outer diameter of the dish cover 1 is smaller than the inner diameter of the annular outer wall 22. The dish cover 1 is sleeved on the annular inner wall 23 of the dish body 2.
[0016] The thickness of the dish cover 1, the annular inner wall 23 and the annular outer wall 22 are all 2 mm, and the gap between the annular outer wall 22 and the annular inner wall 23 is not greater than 7 mm. As long as the spacing between the annular outer wall 22 and the annular inner wall 23 can allow the opening angle of the dish cover to be less than 45°, it will be sufficient.
[0017] When the utility model is used, it is operated in the same way as the conventional culture dish. It is sufficient as long as the distance between the two walls of the outer circle of the dish and the inner circle of the dish can limit the angle of opening the dish cover to less than 45 degrees.
[0018] When performing actual microbial operations, the anti-pollution culture dish of the present invention can be operated just like a conventional culture dish without changing conventional operating habits. In addition, the smaller interval between the annular outer wall 22 and the annular inner wall 23 limits the angle at which the dish cover is opened, thereby minimizing the entry of microbial cells from the air into the present invention and avoiding contamination.
Claims
1. A contamination-proof culture dish for solid culture, comprising a double-ring dish body and a dish cover, characterized in that: The double-circle dish body comprises a dish bottom, an annular outer wall and an annular inner wall; the annular outer wall and the annular inner wall are coaxially fixed on the dish bottom, and the dish cover is sleeved on the annular inner wall of the dish body.
2. The anti-pollution culture dish for solid culture according to claim 1, characterized in that: The annular inner wall is higher than the annular outer wall, the inner diameter of the dish cover is larger than the outer diameter of the annular inner wall, and the outer diameter of the dish cover is smaller than the inner diameter of the annular outer wall.
3. The anti-pollution culture dish for solid culture according to claim 1, characterized in that: The thickness of the dish cover, the annular inner wall and the annular outer wall are all 2 mm, and the gap between the annular outer wall and the annular inner wall is not greater than 7 mm.