Culture medium cryopreservation bottle with spiral air exhaust cover

Abstract

The utility model discloses a culture medium cryopreservation bottle with a spiral air exhaust cover, which comprises a bottle body and the air exhaust cover, the air exhaust cover is in threaded connection with the bottle body, a liquid level area is formed in the bottle body, the air exhaust cover comprises a bottle cap and an air exhaust assembly, the bottle cap is a screw cap, the air exhaust assembly is arranged between the screw cap and the liquid level area, and the air exhaust assembly is connected with the bottle body. The air exhaust assembly comprises an upper gland, a spring column and a lower gland which are sequentially connected, an air hole is formed in the rotary cover, through holes which are communicated are formed in the center of the upper gland, the center of the spring column and the center of the lower gland, and the upper gland and the rotary cover are of an integrally-formed structure. And gas in the bottle sequentially escapes from the lower gland, the spring column, the through hole of the upper gland and the gas hole of the screw cap. The culture medium cryopreservation bottle with the spiral air exhaust cover can solve the air exhaust packaging problem of the culture medium cryopreservation bottle, and is simple in operation, simplified in working procedure and easy to package in batches.

Description

Technical Field

[0001] The utility model relates to the technical field of cell culture vessels, in particular to a culture medium freezing bottle with a spiral air extraction cover. Background Art

[0002] Liquid cell culture medium is a biological reagent widely used in in vitro human and animal cell culture to maintain the activity of cells in the culture dish and provide sufficient nutrients. Therefore, culture medium is indispensable in in vitro cell culture and drug research. The basal culture medium contains at least 90% water, vitamins, amino acids, glucose and inorganic salts, and is contained in PVC cryopreservation bottles, generally 200-500ml large bottles, and the storage conditions are 2-6°C; there are also various culture medium supplements to further meet customized cell culture and make the cell culture results more ideal. The ingredients of these supplements are very diverse, such as antibiotics (used to inhibit bacteria during cell culture), cell growth factors (confidential formulas of various suppliers, with complex ingredients, mainly composed of various proteins or recombinant proteins, which are better than basal culture media and can solve some problems with high culture failure rates), fetal bovine serum (a very important ingredient in the culture medium, with good culture effects. Although there are many alternative serum-free culture media, the product performance is still not as good as that of serum-free culture media), recombinant insulin, etc. Culture medium supplements are also contained in PVC cryopreservation bottles, but the specifications are generally 2-10ml, and the storage conditions are complex, with refrigeration at 2-6°C and freezing at minus 20°C.

[0003] Most of the cryopreservation bottles of basal culture medium are narrow-mouthed, square-bodied containers. The narrow-mouth design is to prevent overflow, splashing, and dust from entering when pouring the culture medium, and the square bottle body is convenient for batch packaging. The cryopreservation bottles of culture medium supplements are relatively standard cylindrical shapes. Because they are relatively small, they do not use a narrow-mouth design, as shown in the schematic diagram of the cryopreservation bottle shown in patent CN202030210407.3.

[0004] However, the storage of liquid culture medium is sensitive to the environment. The components in the culture medium are prone to biochemical reactions with the air, causing deterioration, thereby reducing the performance of the culture medium. This is especially true for culture medium supplements, where active ingredients such as protein, serum, and insulin are extremely susceptible to oxidation. Therefore, when packaging in batches, how to more cost-effectively evacuate the air in the cryopreservation bottle to create a relatively vacuum environment inside the bottle is a difficult problem in the current process. Utility Model Content

[0005] In view of the above-mentioned deficiencies of the current liquid culture medium, the utility model provides a culture medium freezing bottle with a spiral vacuum cover, which can solve the vacuum packaging problem of the culture medium freezing bottle, is simple to operate, simplifies the process, and is easy to package in batches.

[0006] In order to achieve the above object, the embodiment of the utility model adopts the following technical solution:

[0007] A culture medium freezing bottle with a spiral vacuum cover comprises a bottle body and a vacuum cover, wherein the vacuum cover is threadedly connected to the bottle body, a liquid level area is formed in the bottle body, the vacuum cover comprises a bottle cap and a vacuum assembly, the bottle cap is a screw cap, the vacuum assembly is arranged between the screw cap and the liquid level area, the vacuum assembly comprises an upper pressure cover, a spring column and a lower pressure cover connected in sequence, a spring is arranged on the outer ring of the spring column, an air hole is arranged on the screw cap, a connected through hole is arranged in the center of the upper pressure cover, the spring column and the lower pressure cover, the upper pressure cover and the screw cap are an integrally formed structure, and during the process of tightening the screw cap, the gas in the bottle escapes from the lower pressure cover, the spring column, the through hole of the upper pressure cover and the air hole of the screw cap in sequence.

[0008] According to one aspect of the utility model, a groove is provided on the top of the rotary cover, the air hole is provided in the groove, an air valve is provided on the groove, and the air valve is movably connected to the groove.

[0009] According to one aspect of the utility model, the bottom of the bottle body is a flat bottom, and the bottom of the inner wall of the bottle body is a U-shaped bottom.

[0010] According to one aspect of the utility model, an anti-slip pad is provided on the outer side of the bottom of the bottle body.

[0011] According to one aspect of the utility model, the top of the spring is fixedly connected to the bottom of the upper pressure cover, and the bottom of the spring is fixedly connected to the top of the lower pressure cover.

[0012] According to one aspect of the utility model, the lower pressure cover and the upper pressure cover are made of PVC material, and the spring column is made of rubber material.

[0013] According to one aspect of the utility model, a conduit is provided in the air hole, and the conduit passes through the air hole and the through holes in the upper pressure cover, the spring column and the lower pressure cover in sequence, and the gas in the bottle is discharged through the conduit.

[0014] Advantages of the implementation of the utility model: The utility model is designed with a spiral air extraction cover, which includes a bottle cap and an air extraction component. The air extraction component is arranged between the screw cap and the liquid level area. The air extraction component includes an upper pressure cap, a spring column and a lower pressure cap connected in sequence. An air hole is arranged on the screw cap. The centers of the upper pressure cap, the spring column and the lower pressure cap are provided with a connected through hole. The gas in the bottle is discharged through the through hole and the air hole during the process of screwing the lower cap. The operation is simple. Only simple steps are required to complete the air extraction installation, which reduces the risk caused by human operation errors. The overall shape is regular and easy to package in batches. The material can also be recycled, which reduces the impact on the environment. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] In order to more clearly illustrate the technical solutions in the embodiments of the present invention, the following briefly introduces the drawings required for use in the embodiments. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without creative work.

[0016] Figure 1 This is a schematic structural diagram of a culture medium freezing bottle with a spiral vacuum cover according to the utility model;

[0017] Figure 2 It is a structural schematic diagram of the rotary cover described in the utility model;

[0018] Figure 3 The figure is a schematic structural diagram of the anti-slip mat of the utility model.

[0019] In the figure: 1. screw cap; 2. upper pressure cap; 3. spring; 4. spring column; 5. lower pressure cap; 6. liquid level area; 7. bottle body; 8. anti-slip pad; 9. groove; 10. air hole; 11. air valve. DETAILED DESCRIPTION

[0020] In the description of the present invention, it should be understood that the terms "center", "longitudinal", "lateral", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inside" and "outside" etc., indicating orientations or positional relationships, are based on the orientations or positional relationships shown in the accompanying drawings, and are only for the convenience of describing the present invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore should not be understood as a limitation on the present invention.

[0021] In the description of the present invention, it should be noted that, unless otherwise clearly specified and limited, the terms "installed", "connected" and "connected" should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection, or it can be indirectly connected through an intermediate medium, or it can be the internal communication of two components. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood by specific circumstances.

[0022] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.

[0023] The current methods for pumping gas from culture medium cryopreservation bottles are mainly the following:

[0024] (1) No air extraction: Suitable for general, non-customized, and general basal culture media;

[0025] (2) Using an exhaust device: Using an exhaust pump or a vacuum pump to exhaust the air in the cryostat, which requires designing reserved air holes and air valves on the lid of the cryostat, and the operation is relatively cumbersome;

[0026] (3) Filling: Fill the bottle with culture medium liquid. Although this solves the problem of residual air in the bottle, it also causes new problems, such as liquid splashing when opening the lid and pouring, and the bottle cap loosening when the external temperature changes greatly (thermal expansion and contraction).

[0027] (4) Filling in an inert gas environment: Packaging is carried out in a closed operating workshop filled with inert gases such as nitrogen and helium. This method does not require vacuuming, and the air remaining in the cryopreservation bottle is mainly inert gas, which is not easy to react with the culture medium. However, this method is very expensive.

[0028] To solve the above-mentioned problem of vacuum packaging of cryopreservation bottles, Figures 1 to 3 As shown, the utility model provides a culture medium freezing bottle with a spiral vacuum cover, including a bottle body 7 and a vacuum cover. The bottle body 7 is made of PVC material. The vacuum cover is threadedly connected to the bottle body 7. A liquid level area 6 is formed in the bottle body 7, which is an area for storing culture fluid. The space capacity of the liquid level area 6 accounts for 2 / 3 of the capacity of the entire bottle body 7.

[0029] In practical applications, the vacuum cap includes a bottle cap and a vacuum assembly. The bottle cap is a screw cap 1, and the material of the screw cap 1 is PVC. The outer side of the screw cap 1 is provided with vertical lines for easy tightening or loosening. The vacuum assembly is arranged between the screw cap 1 and the liquid level area 6. The vacuum assembly includes an upper pressure cap 2, a spring column 4 and a lower pressure cap 5 connected in sequence. The upper pressure cap 2 and the screw cap 1 are an integrally formed structure. The outer ring of the spring column 4 is provided with a spring 3, which is used for adjustment during the process of tightening the bottle cap to avoid crushing the bottle wall or loosening of the bottle cap caused by thermal expansion and contraction. The top of the spring 3 is fixedly connected to the bottom of the upper pressure cap 2, and the bottom of the spring 3 is fixedly connected to the top of the lower pressure cap 5. The lower pressure cap 5 and the upper pressure cap 2 are made of PVC material, and the material of the spring column 4 is rubber material. The lower pressure cap 5 is used to press out the air in the bottle.

[0030] The screw cap 1 is provided with an air hole 10, and the centers of the upper pressure cap 2, the spring column 4 and the lower pressure cap 5 are provided with a connected through hole. The outer side of the upper pressure cap 2 is provided with a spiral pattern structure, which matches the spiral pattern of the inner wall of the bottle body 7 to further tighten the bottle cap. During the tightening process of the screw cap 1, the gas in the bottle escapes from the lower pressure cap 5, the spring column 4, the through hole of the upper pressure cap 2 and the air hole 10 of the screw cap 1 in turn. A conduit is provided in the air hole 10, and the conduit passes through the air hole 10 and the through holes in the upper pressure cap 2, the spring column 4 and the lower pressure cap 5 in turn, and the gas in the bottle is discharged through the conduit. The inner diameter of the conduit is 0.5-1 mm.

[0031] In practical applications, a groove 9 is provided at the top of the screw cap 1, and an air hole 10 is provided in the groove 9. An air valve 11 is provided on the groove 9. The air valve 11 is movably connected to the groove 9, and is specifically provided with a push-pull rail and a buckle matching the air valve 11, so as to facilitate the horizontal push and pull of the air valve 11. The material of the air valve 11 is PVC material, which is used to close the air hole 10. The air valve 11 is provided with horizontal lines for easy pushing and pulling. When the gas in the bottle is discharged during use, the air valve 11 on the screw cap 1 is pushed to close the air hole 10.

[0032] The bottom of the bottle body 7 is flat, and the bottom of the inner wall of the bottle body 7 is a smooth U-shaped bottom, which can effectively prevent the liquid from remaining at the bottom of the bottle and cannot be poured out. The outer side of the bottom of the bottle body 7 is provided with an anti-skid pad 8, and the pattern on the anti-skid pad 8 is a circular pattern, and the anti-skid pad 8 is attached to the bottom of the bottle body 7.

[0033] The utility model provides a culture medium freezing bottle with a spiral vacuum cover. During use, firstly, 2 / 3 of the volume of culture solution is filled in the bottle body 7, and then the vacuum cover is screwed down clockwise. During the tightening process, the remaining gas in the bottle escapes from the lower pressure cover 5, the spring column 4, the through hole of the upper pressure cover 2, and then to the air hole 10 on the screw cover 1. Finally, the air valve 11 on the screw cover 1 is pushed to close the air hole 10, and the air hole 10 and the through hole are connected by a conduit.

[0034] Since the bottle is in a relative vacuum state, the screw cap is very tight. If you want to open the culture medium cryopreservation bottle, you must first push the air valve to open the air hole, and then rotate the air pump cap counterclockwise to open it. Since there is still some air in the spring area between the upper and lower glands in the bottle, the bottle cap can be prevented from loosening due to thermal expansion and contraction. In addition, the liquid level area is relatively vacuum, and the culture medium is not easy to prepare.

[0035] The utility model can well solve the problem of small-size packaging of culture medium supplements. For large-size packaging, the culture medium freezing bottle adopts a smooth-mouth bottle design. If it is directly poured, it is indeed easy to cause the liquid in the bottle to splash out. However, at present, a pipette gun is generally used to take liquid in a biochemical laboratory, and the laboratory environment should be relatively sterile and dust-free. Therefore, the design of a narrow-mouth bottle is not necessary. The utility model is designed with a spiral vacuum cover, which includes a bottle cap and a vacuum assembly. The vacuum assembly is arranged between the screw cap and the liquid level area. The vacuum assembly includes an upper pressure cover, a spring column and a lower pressure cover connected in sequence. An air hole is provided on the screw cap, and a connected through hole is provided in the center of the upper pressure cover, the spring column and the lower pressure cover. The gas in the bottle is discharged through the through hole and the air hole during the process of screwing the lower cover. The operation is simple, and only simple steps are required to complete the vacuum installation, which reduces the risk caused by human operating errors. The overall shape is regular, easy to package in batches, and the material can also be recycled, reducing the impact on the environment.

[0036] The above is only a specific implementation of the utility model, but the protection scope of the utility model is not limited thereto. Any changes or substitutions that can be easily thought of by any technician familiar with the technical field within the technical scope disclosed in the utility model should be included in the protection scope of the utility model. Therefore, the protection scope of the utility model shall be based on the protection scope of the claims.

Claims

1. A culture medium freezing bottle with a screw-type vacuum cover, comprising a bottle body (7) and a vacuum cover, wherein the vacuum cover is threadedly connected to the bottle body (7), and a liquid level area (6) is formed in the bottle body (7), characterized in that: The vacuum cover comprises a bottle cap and a vacuum assembly, wherein the bottle cap is a screw cap (1), and the vacuum assembly is arranged between the screw cap (1) and a liquid level area (6). The vacuum assembly comprises an upper pressure cap (2), a spring column (4) and a lower pressure cap (5) which are connected in sequence, wherein the outer ring of the spring column (4) is provided with a spring (3), and an air hole (10) is provided on the screw cap (1). The centers of the upper pressure cap (2), the spring column (4) and the lower pressure cap (5) are provided with a connected through hole, and the upper pressure cap (2) and the screw cap (1) are an integrally formed structure. During the process of tightening the screw cap (1), the gas in the bottle escapes from the lower pressure cap (5), the spring column (4), the through hole of the upper pressure cap (2) and the air hole (10) of the screw cap (1) in sequence.

2. The culture medium freezing bottle according to claim 1, characterized in that: The top of the rotary cover (1) is provided with a groove (9), the air hole (10) is provided in the groove (9), the groove (9) is provided with an air valve (11), and the air valve (11) is movably connected to the groove (9).

3. The culture medium freezing bottle according to claim 1, characterized in that: The bottom of the bottle body (7) is a flat bottom, and the bottom of the inner wall of the bottle body (7) is a U-shaped bottom.

4. The culture medium freezing bottle according to claim 1, characterized in that: An anti-slip pad (8) is provided on the outer side of the bottom of the bottle body (7).

5. The culture medium freezing bottle according to claim 1, characterized in that: The top of the spring (3) is fixedly connected to the bottom of the upper pressure cover (2), and the bottom of the spring (3) is fixedly connected to the top of the lower pressure cover (5).

6. The culture medium freezing bottle according to claim 1, characterized in that: The lower pressure cover (5) and the upper pressure cover (2) are made of PVC material, and the spring column (4) is made of rubber material.

7. The culture medium freezing bottle according to claim 1, characterized in that: A conduit is arranged in the air hole (10), and the conduit passes through the air hole (10) and the through holes in the upper gland (2), the spring column (4) and the lower gland (5) in sequence, and the gas in the bottle is discharged through the conduit.

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