Magnetic bead incubation device convenient to assemble

Abstract

The utility model discloses a magnetic bead incubation device convenient to assemble, which comprises a box body and a cover body covering the upper part of the box body, the bottom in the box body is fixedly connected with an oscillator, the upper end of the oscillator is connected with a mounting table, the mounting table is detachably connected with a mixing incubation box, and the bottom of the mixing incubation box is provided with a connecting plate. The mounting table comprises a table body connected with the oscillator and a mounting groove located in the upper portion of the table body, at least two positioning columns which penetrate through the table body and extend into the mounting groove are arranged on one side of the table body, and the positioning columns slide on one side of the table body, penetrate through the mounting groove and extend into positioning holes in the other side of the table body. The connecting plate comprises a plate body matched with the mounting groove and an insertion hole allowing the insertion end of the positioning column to penetrate through. According to the utility model, the mounting table and the connecting plate can be quickly assembled only by operating the push-pull plate, a plurality of test tubes can be uniformly mixed and incubated at the same time, and the uniformly mixing and incubating steps are combined together, so that the uniformly mixing and incubating efficiency of the magnetic beads is improved, the incubation is always in a darkroom and a low-temperature environment, and the combining efficiency of the magnetic beads and sample cells is improved.

Description

Technical Field

[0001] The utility model relates to the technical field of magnetic bead sorting and incubation, in particular to a magnetic bead incubation device which is convenient to assemble. Background Art

[0002] Immunomagnetic bead sorting technology uses magnetic microbeads to magnetically mark target cells, and then enriches the desired cells through sorting columns in a stable high-intensity magnetic field. The purity of the target cells after sorting is very high, reaching more than 90%. High-purity cells can be used for cell culture, cytogenetics, immunology, and molecular biology detection and analysis, and in clinical practice, it is conducive to the early diagnosis and treatment of diseases. For example, the use of CD138 magnetic beads to enrich plasma cells can effectively increase the MM positive detection rate and avoid missed diagnosis; the use of B / T / NK magnetic beads to sort and classify lymphocyte subtypes, and then quantify EBV nucleic acid, can determine the development stage and infection type of chronic active EB virus disease (CAEBV), which is helpful for disease treatment and prognosis evaluation. The magnetic bead sorting technology process mainly includes two stages: incubation (magnetic bead labeling) and sorting. The quality of incubation directly determines the sorting effect. Incubation is generally closely related to temperature, whether it is protected from light, and whether the magnetic beads and samples are mixed.

[0003] In the current magnetic bead incubation process, the sample and magnetic beads need to be added to the test tube in turn and mixed, and then placed in a 2-8°C refrigerator for incubation. The sample and magnetic bead mixing step before incubation is often carried out at room temperature. Although the time is short, it will still affect the binding efficiency of the magnetic beads and the target cells. In addition, the mixing process needs to be mixed by blowing or using an oscillator. Of these two mixing methods, blowing and mixing will cause loss of the sample magnetic bead mixture and reduce the cell yield, so it is generally rarely used. The oscillator mixing process is difficult to control, which can easily cause the test tube to fall out of hand, and it is not convenient to mix multiple test tubes at the same time. Utility Model Content

[0004] In order to solve the technical problems in the prior art that mixing at room temperature affects the binding efficiency of magnetic beads and target cells, and the oscillator mixing process is difficult to control, which easily causes the test tube to fall out of the hand, and it is inconvenient to mix multiple test tubes at the same time, the utility model provides the following technical solutions.

[0005] The utility model discloses a magnetic bead incubation device which is easy to assemble, comprising a box body and a cover body which covers the upper part of the box body, an oscillator is fixedly connected to the bottom of the box body, an upper end of the oscillator is connected to a mounting table, the mounting table is detachably connected to a mixing incubation box with a connecting plate at the bottom, the mounting table comprises a table body connected to the oscillator and a mounting groove located at the upper part of the table body, at least two positioning posts which penetrate the table body and extend into the mounting groove are provided on one side of the table body, the positioning posts slide on one side of the table body and pass through the mounting groove to extend into the positioning holes on the other side of the table body, and the connecting plate comprises a plate body which matches the mounting groove and an insertion hole for the insertion end of the positioning post to pass through.

[0006] As a further technical solution, one end of the positioning column away from the platform is fixedly connected to a push-pull plate, and a spring sleeved on the outer circumference of the positioning column is fixedly connected between the push-pull plate and the platform.

[0007] As a further technical solution, a plurality of test tube holes are provided on the upper portion of the mixing incubator, and a plurality of elastic rings for clamping the test tubes are longitudinally provided in the test tube holes.

[0008] As a further technical solution, the elastic ring is made of silicone material.

[0009] As a further technical solution, a sealing groove is provided at the upper end of the box body, a sealing strip matching the shape of the sealing groove is provided on the lower side of the cover body, a locking tongue is provided on one side of the upper part of the box body, and a locking plate matching the locking tongue is provided on one side of the cover body.

[0010] As a further technical solution, a refrigerant connection port penetrating through the box body and connected to an external refrigerant is provided on the outer side of the box body, and a cold outlet connected to the refrigerant connection port is provided on the inner wall of the box body.

[0011] The utility model has the beneficial effects that an oscillator is fixedly arranged in the box, a mounting platform is connected to the upper part of the oscillator, the mounting platform is connected to a mixing incubation box with a connecting plate at the bottom, the mounting groove at the upper part of the mounting platform and the connecting plate can be quickly matched, and the mounting platform and the connecting plate can be quickly assembled by operating the push-pull plate, and the test tube can be directly inserted into the test tube hole of the mixing incubation box with an elastic ring, and multiple test tubes can be mixed and incubated at the same time, and the mixing and incubation steps are combined to improve the mixing and incubation efficiency of magnetic beads. The cover body is sealed and connected to the box body, and through the refrigerant connection port and the cold outlet connected to the external refrigerant, the incubation is always in a dark room and a low temperature (2-8°C) environment, which greatly improves the combination efficiency of magnetic beads and sample cells. BRIEF DESCRIPTION OF THE DRAWINGS

[0012] Figure 1 It is a schematic diagram of the structure of the magnetic bead incubation device which is easy to assemble in the utility model;

[0013] Figure 2 This is a schematic diagram of the decomposition of the magnetic bead incubation device which is easy to assemble in the utility model;

[0014] Figure 3 It is a schematic diagram of the installation platform of the magnetic bead incubation device which is convenient to assemble in the utility model;

[0015] Figure 4 It is a schematic diagram of a connecting plate of a magnetic bead incubation device that is easy to assemble according to the utility model;

[0016] In the figure: 1-box; 101-sealing groove; 102-locking tongue; 103-refrigerant connection port; 104-cold outlet; 2-cover; 201-sealing strip; 202-locking plate; 3-oscillator; 4-mounting table; 401-table body; 402-mounting groove; 403-push-pull plate; 404-positioning column; 405-insertion end; 406-positioning hole; 407-spring; 5-connecting plate; 501-plate body; 502-insertion hole; 6-mixing incubator; 601-test tube hole; 602-elastic ring. DETAILED DESCRIPTION

[0017] In order to make the purpose, technical solution and advantages of the utility model more clear, the utility model is further described in detail below in conjunction with the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are only used to explain the utility model and are not used to limit the utility model. It should be noted that the embodiments in the utility model and the features in the embodiments can be combined with each other without conflict.

[0018] In the description of the present invention, it should be understood that the terms "upper", "lower" and "both sides" are based on the orientation or positional relationship shown in the drawings, and are only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as limiting the present invention. In addition, the terms "first", "second" and the like are only used for descriptive purposes, and cannot be understood as indicating or implying relative importance or implicitly indicating the number of the indicated technical features.

[0019] like Figure 1 and Figure 2As shown, in a preferred embodiment, the utility model is a conveniently assembled magnetic bead incubation device, comprising a box body 1 and a cover body 2 covering the upper part of the box body 1, a sealing groove 101 is provided at the upper end of the box body 1, and the sealing groove 101 is provided at three edges of the upper end of the box body 1, a sealing strip 201 matching the shape of the sealing groove 101 is provided at the lower side of the cover body 2, a locking tongue 102 is provided at one side of the upper part of the box body 1, and a locking plate 202 matching the locking tongue 102 is provided at one side of the cover body 2. When the cover body 2 covers the upper end of the box body 1, the sealing strip 201 is inserted into the sealing groove 101, and the locking plate 202 matches the locking tongue 102, so that the cover body 2 seals the box body 1.

[0020] The outer side of the box body 1 is provided with a refrigerant connection port 103 that penetrates the box body 1 and is connected to an external refrigerant. The external refrigerant controls the temperature in the box body 1 to be between low temperature (2-8°C). The inner wall of the box body 1 is provided with a cold outlet 104 that is connected to the refrigerant connection port 103. The refrigerant connection port 103 and the cold outlet are two sets that are arranged oppositely. After the cover body 2 covers and seals the box body 1, the box body 1 is in a dark room and low temperature (2-8°C) environment, which can combine the mixing and incubation steps together, greatly improving the efficiency of combining the magnetic beads and sample cells in the test tube.

[0021] In a preferred embodiment, an oscillator 3 is fixedly connected to the bottom of the box 1. The oscillator 3 adopts the existing technology and will not be described in detail. The upper end of the oscillator 3 is connected to a mounting platform 4, and the mounting platform 4 is detachably connected to a mixing incubation box 6. A connecting plate 5 is provided at the bottom of the mixing incubation box 6. The connecting plate 5 can be connected to the bottom of the mixing incubation box 6 by bolts or integral molding. The efficiency of the mixing incubation process can be improved by the quick assembly connection of the mounting platform 4 and the connecting plate 5.

[0022] In a preferred embodiment, the mounting platform 4 includes a platform body 401 connected to the oscillator 3 and a mounting groove 402 located on the upper part of the platform body 401. The mounting groove 402 is arranged along the length direction of the platform body 401 and can accommodate the installation of the connecting plate 5. Two positioning columns 404 are arranged on one side of the platform body 401, which penetrate the platform body 401 and extend into the mounting groove 402. The positioning columns 404 are cylindrical structures, and one end of the positioning columns 404 extending into the mounting groove 402 is provided with an insertion end 405. The positioning columns 404 can slide horizontally on one side wall of the platform body 401 and pass through the mounting groove 402 to extend into the positioning hole 406 on the other side of the platform body 401. The positioning hole 406 is a blind hole, and its shape matches the insertion end 405. The connecting plate 5 includes a plate body 501 matching the mounting groove 402 and an insertion hole 502 for the insertion end 405 of the positioning columns 404 to pass through. Thus, the positioning column 404 can slide and extend to the installation groove 402 on one side wall of the platform 401, and after being inserted into the insertion hole 502, extend from the insertion hole 502 into the positioning hole 406, completing the rapid assembly of the mounting platform 4 and the connecting plate 5, and then the oscillator 3 can be started to mix the mixing incubator 6.

[0023] In a preferred embodiment, one end of the positioning column 404 away from the platform 401 is fixedly connected to the push-pull plate 403, both ends of the push-pull plate 403 are respectively fixedly connected to one end of the positioning column 404, and a spring 407 sleeved on the outer periphery of the positioning column 404 is fixedly connected between the push-pull plate 403 and the platform 401. Under the action of the spring 407, the positioning column 404 is pressed tightly against the positioning hole 406.

[0024] In a preferred embodiment, a plurality of test tube holes 601 are provided on the upper portion of the mixing incubator 6, such as eight in this embodiment, and a plurality of elastic rings 602 for clamping the test tube are longitudinally provided in each test tube hole 601. The elastic ring 602 is made of silicone material and can stably clamp the test tube in the test tube hole 601.

[0025] When the utility model is in use, the push-pull plate 403 is first pulled, and the positioning column 404 moves from the mounting groove 402 to the inner wall of one side of the platform body 401. At this time, the mixing incubation box 6 is placed on the upper part of the mounting platform 4, so that the plate body 501 of the connecting plate 5 matches the mounting groove 402 box. Then, the push-pull plate 403 is loosened, and the positioning column 404 slides and extends to the mounting groove 402 under the action of the spring 407 and is inserted into the insertion hole 502, and extends from the insertion hole 502 to the positioning hole 406, completing the rapid assembly of the mounting platform 4 and the connecting plate 5, and then the sample and magnetic beads are added to the test tube in turn, and multiple test tubes are inserted into the test tube holes 601 of the mixing incubation box 6 in turn, and then the cover body 2 is closed on the upper end of the box body 1, and the external refrigerant is started to make the low temperature (2-8°C) environment in the box body 1, and the oscillator 3 is started at the same time to mix the mixing incubation box 6, and after mixing, the incubation in the box body 1 continues.

[0026] The preferred specific implementation modes and embodiments of the utility model are described in detail above in conjunction with the accompanying drawings, but the utility model is not limited to the above implementation modes and embodiments. Within the knowledge scope of those skilled in the art, various changes or equivalent substitutions can be made without departing from the concept of the utility model. Therefore, the utility model is not limited to the specific embodiments disclosed herein, and all embodiments falling within the scope of the claims of this application belong to the scope protected by the utility model.

Claims

1. A magnetic bead incubation device that is easy to assemble, comprising a box (1) and a cover (2) covering the upper part of the box (1), characterized in that: An oscillator (3) is fixedly connected to the bottom of the box body (1), and a mounting platform (4) is connected to the upper end of the oscillator (3). The mounting platform (4) is detachably connected to a mixing incubator (6) having a connecting plate (5) at the bottom. The mounting platform (4) includes a platform body (401) connected to the oscillator (3) and a mounting groove (402) located at the upper part of the platform body (401). One side of the platform body (401) is provided with at least two positioning columns (404) that penetrate the platform body (401) and extend into the mounting groove (402). The positioning columns (404) slide on one side of the platform body (401) and pass through the mounting groove (402) to extend into the positioning holes (406) on the other side of the platform body (401). The connecting plate (5) includes a plate body (501) that matches the mounting groove (402) and an insertion hole (502) for the insertion end (405) of the positioning column (404) to pass through.

2. The magnetic bead incubation device that is easy to assemble according to claim 1, characterized in that: One end of the positioning column (404) away from the platform (401) is fixedly connected to a push-pull plate (403), and a spring (407) sleeved on the outer periphery of the positioning column (404) is fixedly connected between the push-pull plate (403) and the platform (401).

3. The magnetic bead incubation device that is easy to assemble according to claim 1, characterized in that: The upper portion of the mixing incubator (6) is provided with a plurality of test tube holes (601), and a plurality of elastic rings (602) for clamping the test tubes are longitudinally arranged inside the test tube holes (601).

4. The magnetic bead incubation device that is easy to assemble according to claim 3, characterized in that: The elastic ring (602) is made of silicone material.

5. The conveniently assembled magnetic bead incubation device according to claim 1, characterized in that: The upper end of the box body (1) is provided with a sealing groove (101), the lower side of the cover body (2) is provided with a sealing strip (201) whose shape matches that of the sealing groove (101), one side of the upper part of the box body (1) is provided with a locking tongue (102), and one side of the cover body (2) is provided with a locking plate (202) which matches the locking tongue (102).

6. The conveniently assembled magnetic bead incubation device according to claim 1, characterized in that: The outer side of the box body (1) is provided with a refrigerant connection port (103) penetrating the box body (1) and connected to an external refrigerant, and the inner wall of the box body (1) is provided with a cold outlet (104) connected to the refrigerant connection port (103).

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