Microporous plate for immunoassay

Abstract

The utility model discloses a microwell plate for immunoassay, and relates to the technical field of medical equipment. The device comprises a bottom plate, a main body assembly and a sealing assembly, the main body assembly comprises a mounting plate, detection blocks, a rectangular-ambulatory-plane frame, a transverse frame and an L-shaped plate, the mounting plate is fixedly connected to the center of the upper surface of the bottom plate, the multiple groups of detection blocks are clamped to the upper surface of the mounting plate in a rectangular array distribution mode, and the rectangular-ambulatory-plane frame is fixedly connected to the upper portion of the mounting plate; the sealing assembly comprises a fixing plate, a lifting frame, a mounting frame, a winding roller and a sealing film. According to the utility model, the position of the L-shaped plate is adjusted through the frame shaped like a Chinese character'hui 'and the transverse frame in the main body assembly, and the detection block is fixed on the mounting plate through the L-shaped plate, so that the problem that the number of detection holes is inconvenient to adjust according to actual requirements in the prior art is solved; the problem that an existing sealing structure is inconvenient to clean is solved.

Description

Technical Field

[0001] The utility model belongs to the technical field of medical equipment, in particular to a microplate used for immune testing. Background Art

[0002] Immunology is a biomedical science that studies the immune response of organisms to antigens and their methods. In simple terms, it studies the body's resistance to the external environment. A large number of medical devices are used in the process of immunology testing, among which microplates are the most common medical devices used for immunological testing.

[0003] The existing document with publication number CN220542790U discloses a luminescence detection microplate equipped with a sealing cover, comprising a microplate, a plurality of luminescence detection holes arranged in a matrix are formed on the top of the microplate, a circle of annular sealing protrusions are formed at the top opening of each luminescence detection hole, and a first magnet is embedded on the side wall of the microplate; a sealing cover, the sealing cover constitutes a square cover structure corresponding to the microplate, a flexible sealing gasket is provided at the bottom of the sealing cover, a plurality of annular sealing grooves corresponding to the sealing protrusions are formed on the bottom side of the flexible sealing gasket, and a second magnet is embedded on the side wall of the sealing cover, the second magnet and the first magnet correspond to each other and have opposite magnetic poles;

[0004] However, it still has the following disadvantages in actual use:

[0005] 1. The microplate mentioned above has a plurality of luminescent detection holes arranged in a matrix on the top of the microplate, through which the culture fluid is placed. However, since the luminescent detection holes are directly opened on the microplate, the number of luminescent detection holes is fixed, which makes it inconvenient to freely adjust the number of luminescent detection holes according to actual needs.

[0006] 2. The microplate mentioned above has a circle of annular sealing protrusions formed at the top opening of the luminescence detection hole, and the bottom side of the flexible sealing pad at the bottom of the sealing cover is formed with a plurality of annular sealing grooves that match the sealing protrusions one by one. The luminescence detection holes are sealed by the sealing cover to avoid overflow of the culture solution, but it also increases the workload of cleaning the microplate, thereby affecting the use efficiency of the microplate.

[0007] To this end, we provide a microplate for immunoassay to solve the above problems. Utility Model Content

[0008] The utility model aims to provide a microplate for immunoassay, wherein the position of the L-shaped plate is adjusted by means of a circular frame and a horizontal frame in a main body component, and a detection block is fixed to a mounting plate by means of the L-shaped plate, thereby solving the problem that it is inconvenient to adjust the number of detection holes according to actual needs. The sealing film is driven to be attached to the detection holes by means of a lifting frame and a winding roller in a sealing component, thereby solving the problem that the existing sealing structure is inconvenient to clean.

[0009] In order to solve the above technical problems, the utility model is realized by the following technical solutions:

[0010] The utility model is a microplate for immunoassay, comprising a bottom plate, a main body component and a sealing component;

[0011] The main assembly includes a mounting plate, a detection block, a back-shaped frame, a horizontal frame and an L-shaped plate. The mounting plate is fixedly connected to the center of the upper surface of the bottom plate. The upper surface of the mounting plate is connected with multiple groups of detection blocks in a rectangular array. The top of the mounting plate is fixedly connected with a back-shaped frame. The front and rear ends of the inner side of the back-shaped frame are movably connected with the horizontal frame. The two sides of the end of the horizontal frame close to each other are movably connected with the L-shaped plate.

[0012] The sealing assembly includes a fixed plate, a lifting frame, an installation frame, a winding roller and a sealing film. The fixed plate is fixedly connected to both sides of the upper surface of the base plate, and the front and rear ends of the upper surface of the fixed plate are fixedly connected to the lifting frame and the installation frame respectively. A winding roller is longitudinally rotatably connected between the lifting frame and the installation frame, and a sealing film is wrapped around the outer wall of the winding roller.

[0013] The utility model is further configured as follows: a plurality of positioning holes are provided on the upper surface of the mounting plate in a rectangular array, positioning posts are provided in the internal gaps of the positioning holes, the tops of the positioning posts are welded to the bottom of the detection blocks, and detection holes are provided on the tops of the detection blocks.

[0014] The utility model is further configured as follows: sliding grooves are provided on the inner walls on both sides of the return frame, the inner side of the sliding groove is connected to a first screw rod for longitudinal rotation, the front and rear ends of the outer wall of the first screw rod are threadedly sleeved with a first slider, one side of the first slider is respectively welded to the outer walls on both sides of the horizontal frame, the inside of the horizontal frame is connected to a second screw rod for transverse rotation, the outer wall of the second screw rod is threadedly sleeved with a second slider on both sides, and one side of the second slider is welded to the horizontal support arm of the L-shaped plate.

[0015] The utility model is further configured as follows: two ends of the first screw rod respectively rotate to penetrate the front and rear ends of the slide slot and are respectively fixedly connected with the first knob and the synchronous wheel; the first knob and the synchronous wheel are respectively located at the front and rear ends of the return frame, and the synchronous wheels are connected by belt transmission.

[0016] The utility model is further configured as follows: a rotating shaft is rotatably passed through the top center position of the horizontal frame, a second knob is fixedly connected to the top end of the rotating shaft, a bevel gear is sleeved on the outer wall center position of the second screw rod and the bottom end of the rotating shaft, and the bevel gears are meshed and connected with each other.

[0017] The utility model is further configured as follows: a third slider is vertically movably connected inside the lifting frame, the front end of the winding roller is rotatably connected to the rear end surface of the third slider, a sleeve rod passes through the top of the lifting frame, a threaded rod is vertically threaded inside the sleeve rod, a third knob is fixedly connected to the top of the threaded rod, and the bottom end of the threaded rod is rotatably connected to the top of the third slider.

[0018] The utility model is further configured as follows: a fourth slider is vertically movably connected inside the installation frame, the rear end of the winding roller is rotatably connected to the front end surface of the fourth slider, grooves are vertically opened on the inner walls on both sides of the installation frame, and protrusions are movably connected inside the grooves, one side of the protrusions passes through the grooves and is respectively welded to the outer walls on both sides of the fourth slider.

[0019] The utility model is further configured as follows: torsion springs are sleeved on the outer sides of the front and rear ends of the winding roller.

[0020] The utility model has the following beneficial effects:

[0021] 1. The utility model sets a main body component, and the detection block is positioned at a specified position on the mounting plate through the clearance fit between the positioning column and the positioning hole, and the L-shaped plate is driven to move through the threaded connection between the first screw rod, the second screw rod and the first slider and the second slider, so that the L-shaped plate is clamped at the four corners of the detection block, thereby fixing the detection block, and making it convenient for the staff to adjust the number of detection holes according to actual needs.

[0022] 2. The utility model sets a sealing component, drives the winding roller and the sealing film to rise and fall through the third slider in the lifting frame, and winds up the two ends of the sealing film through the winding roller, so that the sealing film fits tightly on the detection block. The setting of the sealing film can prevent the culture solution in the detection hole from overflowing, and when cleaning is needed, only the sealing film needs to be replaced.

[0023] Of course, any product implementing the present invention does not necessarily need to achieve all of the advantages described above at the same time. BRIEF DESCRIPTION OF THE DRAWINGS

[0024] In order to more clearly illustrate the technical solutions of the embodiments of the utility model, the drawings required for describing the embodiments are briefly introduced below. Obviously, the drawings described below are only some embodiments of the utility model. For ordinary technicians in this field, other drawings can be obtained based on these drawings without creative work.

[0025] Figure 1 The figure is a schematic diagram of the overall structure of a microplate used for immunoassay;

[0026] Figure 2 It is a structural schematic diagram of the main components of the utility model;

[0027] Figure 3 This is a schematic diagram of the structure of the mounting plate of the utility model;

[0028] Figure 4 This is a schematic diagram of the structure of the detection block of the utility model;

[0029] Figure 5 It is a schematic diagram of the installation between the horizontal frame and the L-shaped plate of the utility model;

[0030] Figure 6 This is a schematic diagram of the structure of the second screw rod of the utility model;

[0031] Figure 7 This is a schematic diagram of the structure of the sealing component of the utility model;

[0032] Figure 8 This is a structural schematic diagram of the lifting frame of the utility model;

[0033] Fig. 9 This is a structural disassembly diagram of the installation frame of the utility model;

[0034] Fig.10 It is a structural schematic diagram of the winding roller of the utility model.

[0035] In the accompanying drawings, the components represented by the reference numerals are listed as follows:

[0036] 1-bottom plate, 2-main assembly, 201-mounting plate, 201a-positioning hole, 202-detection block, 202a-positioning column, 202b-detection hole, 203-return frame, 203a-slideway, 204-horizontal frame, 205-L-shaped plate, 206-first screw rod, 206a-first knob, 206b-synchronous wheel, 206c-belt, 207-first slider, 208-second screw rod, 208a-rotating shaft , 208b-second knob, 208c-conical gear, 209-second slider, 3-sealing assembly, 301-fixed plate, 302-lifting frame, 302a-third slider, 302b-sleeve rod, 302c-threaded rod, 302d-third knob, 303-installing frame, 303a-fourth slider, 303b-groove, 303c-bump, 304-winding roller, 304a-torsion spring, 305-sealing film. DETAILED DESCRIPTION

[0037] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments of the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model. Specific embodiment 1

[0039] See also Figure 1-6 As shown, it is the first embodiment of the utility model, which provides a microplate for immunoassay, including a base plate 1 and a main body component 2, and the main body component 2 includes a mounting plate 201, a detection block 202, a circular frame 203, a horizontal frame 204 and an L-shaped plate 205. The L-shaped plate 205 is driven to move by the circular frame 203 and the horizontal frame 204, and the detection block 202 is fixed to the mounting plate 201 by the L-shaped plate 205, which solves the existing problem of being inconvenient to adjust the number of detection holes 202b according to actual needs.

[0040] Specifically, the mounting plate 201 is fixedly connected to the center position of the upper surface of the base plate 1, and the upper surface of the mounting plate 201 is distributed in a rectangular array and is connected with multiple groups of detection blocks 202. A circular frame 203 is fixedly connected to the top of the mounting plate 201, and the front and rear ends of the inner sides of the circular frame 203 are movably connected to a horizontal frame 204, and both sides of the ends of the horizontal frame 204 that are close to each other are movably connected to an L-shaped plate 205. The mounting plate 201 is used to install structures such as the circular frame 203, and the detection block 202 is used to hold the culture solution. The circular frame 203 is used to movably install the horizontal frame 204, and the horizontal frame 204 is used to movably install the L-shaped plate 205, and the detection block 202 is clamped and fixed by the L-shaped plate 205.

[0041] Furthermore, the upper surface of the mounting plate 201 is provided with a plurality of positioning holes 201a in a rectangular array, the internal gaps of the positioning holes 201a are matched with positioning posts 202a, the tops of the positioning posts 202a are welded to the bottom of the detection block 202, and the tops of the detection blocks 202 are provided with detection holes 202b, and the arrangement of the positioning holes 201a and the positioning posts 202a realizes the positioning between the mounting plate 201 and the detection block 202;

[0042] Slide grooves 203a are provided on the inner walls of both sides of the return frame 203, and the inner side of the slide groove 203a is connected to the first screw rod 206 for longitudinal rotation. The front and rear ends of the outer wall of the first screw rod 206 are threadedly sleeved with first sliders 207, and one side of the first slider 207 is respectively welded to the outer walls of both sides of the cross frame 204, and the inside of the cross frame 204 is connected to the second screw rod 208 for transverse rotation. The outer wall of the second screw rod 208 is threadedly sleeved with second sliders 209 on both sides, and one side of the second slider 209 is welded to the transverse support arm of the L-shaped plate 205. The slide groove 203a is used to install the first screw rod 206, and the first screw rod 206 and the first slider 207 are used to drive the cross frame 204 to move toward or away from each other in the front and rear directions, and at the same time, the threaded connection between the second screw rod 208 and the second slider 209 drives the L-shaped plate 205 to move toward or away from each other in the left and right directions.

[0043] The two ends of the first screw rod 206 rotate through the front and rear ends of the slide slot 203a and are respectively fixedly connected with the first knob 206a and the synchronous wheel 206b. The first knob 206a and the synchronous wheel 206b are respectively located at the front and rear ends of the return frame 203. The synchronous wheels 206b are connected by a belt 206c. The first knob 206a is used to rotate the first screw rod 206, and the synchronous wheel 206b and the belt 206c are used to drive the two groups of first screw rods 206 to rotate synchronously.

[0044] A rotating shaft 208a is rotatably passed through the top center position of the horizontal frame 204, and a second knob 208b is fixedly connected to the top of the rotating shaft 208a. A bevel gear 208c is sleeved on the outer wall center position of the second screw rod 208 and the bottom end of the rotating shaft 208a. The bevel gears 208c are meshed with each other. The rotating shaft 208a is used to drive the bevel gear 208c to rotate, and the meshing transmission between the bevel gears 208c drives the second screw rod 208 to rotate. At the same time, the second knob 208b is configured to facilitate the rotation of the rotating shaft 208a.

[0045] The operation process of this embodiment is as follows: first, the number of detection blocks 202 is selected according to actual needs, and the detection block 202 is positioned on the mounting plate 201 through the clearance fit between the positioning column 202a and the positioning hole 201a, and then the first knob 206a and the second knob 208b are rotated, and the first knob 206a and the second knob 208b respectively drive the first screw rod 206 and the rotating shaft 208a to rotate synchronously, and under the action of the threaded connection between the first screw rod 206 and the first slider 207, the first slider 207 drives the cross frame 204 to move toward or away from each other in the front and rear directions, thereby The lateral support arms of the L-shaped plate 205 are in contact with the front and rear ends of the detection block 202. At the same time, the rotating shaft 208a drives the second screw rod 208 to rotate synchronously through the bevel gear 208c. Under the action of the threaded connection between the second screw rod 208 and the second slider 209, the L-shaped plate 205 moves towards or away from each other in the left and right directions, and then the longitudinal support arms of the L-shaped plate 205 are respectively in contact with the two sides of the detection block 202, thereby fixing the detection block 202. Finally, the culture fluid is placed in the detection hole 202b on the top of the detection block 202, and the sample for immunoassay is placed in the culture fluid. Specific embodiment 2

[0047] See also Figure 7-10 As shown, it is the second embodiment of the utility model, which is based on the previous embodiment, but is different from the previous embodiment in that it also includes a sealing component 3, and the sealing component 3 includes a fixed plate 301, a lifting frame 302, an installation frame 303, a winding roller 304 and a sealing film 305. The sealing film 305 is driven by the lifting frame 302 and the winding roller 304 to adhere to the main component 2, which solves the existing problem of inconvenience in cleaning and replacing the sealing structure.

[0048] Specifically, the fixed plate 301 is fixedly connected to both sides of the upper surface of the base plate 1, and the front end and the rear end of the upper surface of the fixed plate 301 are fixedly connected with the lifting frame 302 and the installation frame 303 respectively. A winding roller 304 is connected between the lifting frame 302 and the installation frame 303 in a longitudinal rotation manner, and a sealing film 305 is wrapped around the outer wall of the winding roller 304. The setting of the fixed plate 301 is used to install structures such as the lifting frame 302 and the installation frame 303. The setting of the lifting frame 302 is used to drive the winding roller 304 to rise and fall, and the setting of the installation frame 303 makes the lifting and falling of the winding roller 304 more stable. At the same time, the setting of the winding roller 304 is used to roll up the two ends of the sealing film 305, and the sealing film 305 is used to seal the culture medium.

[0049] Furthermore, a third slider 302a is movably connected vertically inside the lifting frame 302, and the front end of the winding roller 304 is rotatably connected to the rear end surface of the third slider 302a. A sleeve rod 302b runs through the top of the lifting frame 302, and a threaded rod 302c is vertically threaded inside the sleeve rod 302b. A third knob 302d is fixedly connected to the top of the threaded rod 302c, and the bottom end of the threaded rod 302c is rotatably connected to the top of the third slider 302a. The third slider 302a is used to install the front end of the winding roller 304, and the sleeve rod 302b and the threaded rod 302c are used to drive the third slider 302a to slide, thereby driving the winding roller 304 to rise and fall, and the third knob 302d is convenient for rotating the threaded rod 302c.

[0050] A fourth slider 303a is movably connected vertically inside the installation frame 303, and the rear end of the winding roller 304 is rotatably connected to the front end surface of the fourth slider 303a. Grooves 303b are vertically provided on the inner walls on both sides of the installation frame 303, and bumps 303c are movably connected inside the grooves 303b. One side of the bumps 303c passes through the grooves 303b and is respectively welded to the outer walls on both sides of the fourth slider 303a. The fourth slider 303a is used to rotatably install the rear end of the winding roller 304, and the grooves 303b and the bumps 303c are used to slide the fourth slider 303a inside the installation frame 303.

[0051] Torsion springs 304a are sleeved on the outside of the front and rear ends of the winding roller 304, one end of the torsion spring 304a is fixed on the winding roller 304, and the other end of the torsion spring 304a is respectively fixed on the third slider 302a and the fourth slider 303a. The setting of the torsion spring 304a realizes the automatic reset of the winding roller 304.

[0052] The rest of the structure is the same as that of the first embodiment.

[0053] The operation process of this embodiment is: first, rotate the winding roller 304, and wind the two ends of the sealing film 305 on the winding roller 304 respectively, then rotate the third knob 302d, the third knob 302d drives the threaded rod 302c to rotate synchronously, and under the action of the threaded connection between the threaded rod 302c and the sleeve rod 302b, the threaded rod 302c drives the third slider 302a to rise and fall, thereby driving the winding roller 304 and the sealing film 305 to rise and fall, so that the sealing film 305 is attached to the storage structure of the culture solution, and finally release the winding roller 304. At this time, under the action of the torsion spring 304a, the winding roller 304 rotates to the initial position, so that the two ends of the sealing film 305 are rolled up by the winding roller 304, so that the sealing film 305 is tightly attached to the storage structure of the culture solution.

[0054] In the description of this specification, the description with reference to the terms "one embodiment", "example", "specific example", etc. means that the specific features, structures, materials or characteristics described in conjunction with the embodiment or example are included in at least one embodiment or example of the utility model. In this specification, the schematic representation of the above terms does not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials or characteristics described can be combined in any one or more embodiments or examples in a suitable manner.

[0055] The preferred embodiments of the utility model disclosed above are only used to help explain the utility model. The preferred embodiments do not describe all the details in detail, nor do they limit the utility model to the specific implementation methods described. Obviously, many modifications and changes can be made according to the content of this specification. This specification selects and specifically describes these embodiments in order to better explain the principles and practical applications of the utility model, so that technicians in the relevant technical field can well understand and use the utility model. The utility model is limited only by the claims and their full scope and equivalents.

Claims

1. A microplate for immunoassay, comprising a base plate (1), a main body component (2) and a sealing component (3), characterized in that: The main body component (2) comprises a mounting plate (201), a detection block (202), a back-shaped frame (203), a horizontal frame (204) and an L-shaped plate (205), wherein the mounting plate (201) is fixedly connected to the center position of the upper surface of the bottom plate (1), the upper surface of the mounting plate (201) is arranged in a rectangular array and is connected with a plurality of groups of detection blocks (202), and the top of the mounting plate (201) is fixedly connected with the back-shaped frame (203), the front and rear ends of the inner side of the back-shaped frame (203) are both movably connected with the horizontal frame (204), and the two sides of the mutually adjacent ends of the horizontal frame (204) are both movably connected with the L-shaped plate (205); The sealing assembly (3) comprises a fixed plate (301), a lifting frame (302), an installation frame (303), a winding roller (304) and a sealing film (305), wherein the fixed plate (301) is fixedly connected to both sides of the upper surface of the bottom plate (1), the front end and the rear end of the upper surface of the fixed plate (301) are fixedly connected to the lifting frame (302) and the installation frame (303), respectively, and a winding roller (304) is rotatably connected between the lifting frame (302) and the installation frame (303) in the longitudinal direction, and the sealing film (305) is wound on the outer wall of the winding roller (304).

2. A microplate for immunoassay according to claim 1, characterized in that: The upper surface of the mounting plate (201) is provided with a plurality of positioning holes (201a) in a rectangular array, and the internal gaps of the positioning holes (201a) are matched with positioning columns (202a), the top ends of the positioning columns (202a) are welded to the bottom of the detection block (202), and the tops of the detection blocks (202) are provided with detection holes (202b).

3. A microplate for immunoassay according to claim 1, characterized in that: The inner walls of both sides of the letter-returning frame (203) are provided with sliding grooves (203a), and the inner side of the sliding groove (203a) is connected to a first screw rod (206) for longitudinal rotation, the front and rear ends of the outer wall of the first screw rod (206) are threadedly sleeved with a first slider (207), and one side of the first slider (207) is respectively welded to the outer walls of both sides of the horizontal frame (204), the inside of the horizontal frame (204) is connected to a second screw rod (208) for transverse rotation, and the outer walls of the second screw rod (208) are threadedly sleeved with a second slider (209) on both sides, and one side of the second slider (209) is welded to the horizontal support arm of the L-shaped plate (205).

4. A microplate for immunoassay according to claim 3, characterized in that: The two ends of the first screw rod (206) are respectively rotated to pass through the front and rear ends of the slide groove (203a) and are respectively fixedly connected with a first knob (206a) and a synchronous wheel (206b), and the first knob (206a) and the synchronous wheel (206b) are respectively located at the front and rear ends of the return word frame (203), and the synchronous wheels (206b) are connected by a belt (206c).

5. A microplate for immunoassay according to claim 3, characterized in that: A rotating shaft (208a) is rotatably passed through the top center position of the horizontal frame (204), and a second knob (208b) is fixedly connected to the top of the rotating shaft (208a), and a bevel gear (208c) is sleeved on the center position of the outer wall of the second screw rod (208) and the bottom end of the rotating shaft (208a), and the bevel gears (208c) are meshed and connected with each other.

6. A microplate for immunoassay according to claim 1, characterized in that: The lifting frame (302) is vertically movably connected to a third slider (302a) inside, the front end of the winding roller (304) is rotatably connected to the rear end surface of the third slider (302a), a sleeve rod (302b) passes through the top of the lifting frame (302), and a threaded rod (302c) is vertically threadedly connected inside the sleeve rod (302b), the top of the threaded rod (302c) is fixedly connected to a third knob (302d), and the bottom end of the threaded rod (302c) is rotatably connected to the top of the third slider (302a).

7. A microplate for immunoassay according to claim 1, characterized in that: A fourth slider (303a) is movably connected to the interior of the installation frame (303) in the vertical direction, and the rear end of the winding roller (304) is rotatably connected to the front end surface of the fourth slider (303a). Grooves (303b) are vertically provided on the inner walls on both sides of the installation frame (303), and a protrusion (303c) is movably connected to the interior of the groove (303b), and one side of the protrusion (303c) passes through the groove (303b) and is respectively welded to the outer walls on both sides of the fourth slider (303a).

8. A microplate for immunoassay according to claim 1, characterized in that: Torsion springs (304a) are sleeved on the outer sides of the front and rear ends of the winding roller (304).

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