Filtering device for pretreatment of detection sample
By designing a filter device that includes a filter cartridge, a filter screen, an L-shaped fixing plate, a clamping component, and a limiting component, the problem in the existing technology that sample pretreatment and filtration operations are cumbersome and can easily lead to sample loss and contamination is solved. It achieves high-efficiency filtration of filtrate and effective protection of sample cells.
Patent Information
- Application Number
- CN202420755069.4
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-04-12
- Publication Date
- 2025-01-17
- Estimated Expiration
- 2034-04-12
AI Technical Summary
In the prior art, the filtering process of pre-processing of samples is filtered using a nylon mesh. The operation is complicated and it is easy to cause liquid leakage, resulting in sample loss and contamination; the filter connected to the externally large and unsolid connection is easy to fall off and cause sample overflow. The drop direction of the filtrate is difficult to control and is easily attached to the wall of the test tube, resulting in cell loss.
A filter device is designed that includes a filter cartridge, a filter screen, an L-shaped fixed plate, a clamping component and a limiting component. The bottom of the filter cartridge is a through-structure, the filter screen is set in a conical shape, and the clamping component and the limiting component are used for Stably fix the filter cartridge in the test tube to ensure that the filtrate is filtered along the circumferential slope of the conical filter screen and drips out from the through hole at the bottom of the filter cartridge.
Through this device, the dripping path of the filtrate is greatly shortened, which prevents the filtrate from adhering to the wall of the test tube in large quantities and reduces the loss of sample cells; the stable fixation of the filter cartridge ensures the safety and efficiency of the filtration operation and improves the filtration of the filtrate. Effect.
Smart Images
Figure CN222358510U_ABST
Abstract
Description
Technical Field
[0001] The present application relates to the field of medical detection technology, and in particular to a filtering device for pre-treatment of detection samples. Background Art
[0002] Flow cytometry is an advanced method that can quantitatively analyze single cells. It has the advantages of rapidity, high precision and multi-parameters. It is widely used in clinical and scientific research fields, especially in immunology, hematology, clinical oncology, etc. However, before the flow cytometer is used for detection, the pretreatment of the test sample is crucial, especially the process of preparing a single cell suspension, in order to avoid signal interference and instrument damage caused by cell aggregation.
[0003] At present, nylon mesh is often used for filtration in the sample pre-treatment process in the laboratory. The operation is cumbersome and can easily lead to liquid leakage, resulting in sample loss and contamination. In addition, the externally docked filter is large in size and the connection is not firm, so it is easy to fall off and cause sample spillage. In addition, this external docking method makes it difficult to control the direction of the filtrate falling, and it is easy to adhere to the wall of the test tube, which will cause cell loss for samples with limited cell quantity. Utility Model Content
[0004] In view of the shortcomings of the prior art, the purpose of the present application is to provide a filtering device for pre-treatment of detection samples to solve the problems raised in the above background technology.
[0005] According to one aspect of the present application, a filtering device for pre-processing a sample for detection includes a filter cartridge, a filter screen, an L-shaped fixing plate, a clamping assembly and a limit assembly, wherein the bottom of the filter cartridge is a through structure, a filter screen is fixedly provided on the inner side wall of the filter cartridge near the bottom position, and L-shaped fixing plates are symmetrically provided on both sides of the top of the filter cartridge, the horizontal portion of the L-shaped fixing plate is placed at the position of the tube mouth of the detection test tube, the vertical portion of the L-shaped fixing plate is parallel to the axial direction of the tube body of the detection test tube, and a clamping assembly is slidably provided on the vertical portion of each of the L-shaped fixing plates toward the filter cartridge, and the clamping assemblies arranged on both sides of the filter cartridge are symmetrically arranged, the clamping assembly is used to clamp and fix the detection test tube, a limit assembly is slidably provided on each of the L-shaped fixing plates at a position below the clamping assembly, and the limit assemblies arranged on both sides of the filter cartridge are symmetrically arranged, and the ends of the limit assemblies are in contact with the outer side walls of the detection test tube.
[0006] Preferably, the filter screen is a conical structure, and the axis of the filter screen coincides with the axis of the filter cartridge, and the circular end of the filter screen is fixed on the inner wall of the filter cartridge, and the tip of the filter screen extends toward the through-hole at the bottom of the filter cartridge.
[0007] Preferably, the clamping assembly includes a push-pull rod, a hand-held plate, an arc-shaped clamping plate and a spring, the push-pull rod is vertically slidably arranged on the vertical portion of the L-shaped fixing plate, a hand-held plate is fixedly arranged on one end of the push-pull rod located on the outside of the vertical portion of the L-shaped fixing plate, and an arc-shaped clamping plate is fixedly arranged on one end of the push-pull rod located on the inside of the vertical portion of the L-shaped fixing plate, the inner arc surface of the arc-shaped clamping plate is arranged toward the filter cartridge, a spring is sleeved on the outside of the rod body of the push-pull rod located between the arc-shaped clamping plate and the vertical portion of the L-shaped fixing plate, and both ends of the spring respectively contact the arc-shaped clamping plate and the vertical portion of the L-shaped fixing plate.
[0008] Preferably, a rubber pad is fixedly provided on the inner arc surface of the arc-shaped clamping plate.
[0009] Preferably, two push-pull rods are provided on each vertical portion of the L-shaped fixing plate along its vertical direction.
[0010] Preferably, the limit assembly includes an adjusting threaded rod, a rubber contact and a fixing nut, and a fixing nut is fixedly provided on the side of each L-shaped fixing plate located below the clamping assembly, the fixing nut is internally threaded with an adjusting threaded rod, and a rotating head is fixedly provided at one end of the adjusting threaded rod located on the outside of the vertical part of the L-shaped fixing plate, and a rubber contact is fixedly provided at one end of the adjusting threaded rod located on the inside of the vertical part of the L-shaped fixing plate, and the rubber contact is arranged toward the filter cartridge.
[0011] Preferably, a shielding flare is provided at the opening edge of the top of the filter cartridge and extends toward the outside thereof, and the inner diameter of the shielding flare is larger than the inner diameter of the detection test tube.
[0012] Compared with the prior art, the advantages of the present application are as follows: a filtering device for sample pretreatment of the present application, through the setting of the filter cartridge extension structure, during the filtering operation, the through-hole at the bottom end of the filter cartridge is extended into the test tube, and a filter screen is arranged at the through-hole at the bottom end of the filter cartridge, so that the path of the filtrate dripping from the filter screen to the bottom of the test tube is greatly shortened, thereby avoiding the loss of sample cells caused by a large amount of filtrate adhering to the wall of the test tube; through the clamping assembly and the limiting assembly symmetrically arranged on both sides of the filter cartridge, when the filter cartridge is extended into the test tube, the hand-held plates in the clamping assemblies on both sides are pulled outward, and then the push-pull rod drives the arc-shaped clamping plate to overcome the elastic force of the spring and move toward the outside of the test tube; when the horizontal part of the L-shaped fixing plate is placed at the position of the test tube orifice, the hand-held plate is released, and then the elastic restoring force of the spring pushes the arc-shaped clamping plate to clamp and fix it on the outer wall of the test tube, so that the filter cartridge can be stably arranged in the test tube. In addition, when the clamping assembly clamps After the holding is completed, the rotating heads at the ends of the adjusting threaded rods in the limit assemblies on both sides of the filter cartridge are twisted at the same time, and then the adjusting threaded rod is screwed in, thereby driving the rubber contact on the other end thereof to move and contact the outer wall of the test tube, so that the body of the filter cartridge is located at the central axis position inside the test tube, thereby avoiding the outer wall of the filter cartridge from contacting the inner wall of the test tube; by setting the filter screen to a conical structure, and the tip of the conical filter screen extends to the through-hole at the bottom of the filter cartridge, so that the filtrate can flow and filter along the circumferential inclined surface of the conical filter screen when flowing downward in the filter cartridge and drip out from the through-hole at the bottom of the filter cartridge, so that the filtrate in the filter cartridge can flow through the conical filter screen as much as possible, thereby improving the filtering effect of the filtrate in the filter cartridge; by setting a shielding expansion at the top opening of the filter cartridge, and the inner diameter size of the shielding expansion is larger than the inner diameter size of the test tube, it is possible to avoid the filtrate from dripping directly into the test tube from the gap between the filter cartridge and the test tube to affect the quality of the test sample. BRIEF DESCRIPTION OF THE DRAWINGS
[0013] Figure 1 It is a stereoscopic diagram of a filtering device for pre-treatment of detection samples according to an embodiment of the present application.
[0014] Figure 2 It is a cross-sectional view of a filtering device for pre-treatment of detection samples according to an embodiment of the present application.
[0015] Figure 3 It is a structural schematic diagram of a conical filter screen of a filtering device for pre-treatment of detection samples according to an embodiment of the present application.
[0016] Figure numerals: 1. filter cartridge; 2. filter screen; 3. L-shaped fixing plate; 4. clamping assembly; 41. push-pull rod; 42. hand-held plate; 43. arc-shaped clamping plate; 44. spring; 45. rubber pad; 5. limit assembly; 51. adjusting threaded rod; 52. rubber contact; 53. fixing nut; 54. rotating head; 6. inspection test tube; 7. shielding flare. DETAILED DESCRIPTION
[0017] In order to make the content of this application easier to understand, the technical solutions in the embodiments of this application will be described clearly and completely in conjunction with the drawings in the embodiments of this application. It should be noted that the words "front", "rear", "left", "right", "upper" and "lower" used in the following description refer to the attached drawings. Figure 2 As used herein, the terms "inward" and "outward" refer to directions toward and away from, respectively, the geometric center of the particular component.
[0018] like Figure 1 to Figure 3 As shown, a filtering device for sample pretreatment includes a filter cartridge 1, a filter screen 2, an L-shaped fixing plate 3, a clamping assembly 4 and a limiting assembly 5. The bottom of the filter cartridge 1 is a through structure, and a filter screen 2 is fixedly provided on the inner side wall of the filter cartridge 1 near the bottom. Specifically, the filter screen 2 is a conical structure (such as Figure 3As shown in the figure, the axis of the filter screen 2 coincides with the axis of the filter cartridge 1, and the circular end of the filter screen 2 is fixedly arranged on the inner wall of the filter cartridge 1, and the tip of the filter screen 2 extends toward the bottom through-hole of the filter cartridge 1. In this design, the filter screen 2 is set as a conical structure, and the tip of the conical filter screen 2 extends to the bottom through-hole of the filter cartridge 1, so that the filtrate can flow and filter along the circumferential inclined surface of the conical filter screen 2 when flowing downward in the filter cartridge 1, and drip out from the bottom through-hole of the filter cartridge 1, so that the filtrate in the filter cartridge 1 can be The filter cartridge 1 is provided with an L-shaped fixing plate 3, which is symmetrically arranged on both sides of the top of the filter cartridge 1. The L-shaped fixing plate 3 is symmetrically arranged on both sides of the top of the filter cartridge 1. The L-shaped fixing plate 3 is symmetrically arranged on both sides of the top of the filter cartridge 1. The L-shaped fixing plate 3 is symmetrically arranged on both sides of the top of the filter cartridge 1. The L-shaped fixing plate 3 is symmetrically arranged on both sides of the top of the filter cartridge 1. The horizontal part is placed at the position of the test tube 6, the vertical part of the L-shaped fixing plate 3 is parallel to the axial direction of the test tube 6, and a clamping assembly 4 is provided on the vertical part of each L-shaped fixing plate 3 to slide toward the filter cartridge 1, and the clamping assemblies 4 arranged on both sides of the filter cartridge 1 are symmetrically arranged, and the clamping assemblies 4 are used to clamp and fix the test tube 6, and a limit assembly 5 is threadedly connected at a position below the clamping assembly 4 on each L-shaped fixing plate 3, and the limit assemblies 5 arranged on both sides of the filter cartridge 1 are symmetrically arranged, and the limit assemblies 5 are arranged on the bottom of the filter cartridge 1. The end portion abuts against the outer wall of the detection test tube 6. In this design, through the clamping assembly 4 and the limiting assembly 5 symmetrically arranged on both sides of the filter cartridge 1, after the filter cartridge 1 is inserted into the detection test tube 6, the clamping assembly 4 can stably place the filter cartridge 1 in the detection test tube 6, and the limiting assembly 5 can adjust and limit the position of the filter cartridge 1 inside the detection test tube 6, so that the body of the filter cartridge 1 is located at the center axis position inside the detection test tube 6, thereby avoiding the outer wall of the filter cartridge 1 from contacting the inner wall of the detection test tube 6.
[0019] In one embodiment, in combination Figure 2 and Figure 3The clamping assembly 4 includes a push-pull rod 41, a hand-held plate 42, an arc-shaped clamping plate 43 and a spring 44. The push-pull rod 41 is vertically slidably arranged on the vertical part of the L-shaped fixing plate 3, and two push-pull rods 41 are arranged on the vertical part of each L-shaped fixing plate 3 along its vertical direction, so that the entire clamping assembly 4 is more stable when sliding. The two push-pull rods 41 are located on the outer side of the vertical part of the L-shaped fixing plate 3. One end of the push-pull rod 41 is fixed with a hand-held plate 42, and one end of the push-pull rod 41 is located on the inner side of the vertical part of the L-shaped fixing plate 3. The inner arc surface of the arc-shaped clamping plate 43 is arranged toward the filter cartridge 1, and a rubber pad 45 is fixed on the inner arc surface of the arc-shaped clamping plate 43. The arrangement of the rubber pad 45 can protect the outer side wall of the detection test tube 6 when clamping the detection test tube 6, so as to prevent the arc-shaped clamping plate 43 from causing damage to the outer side wall of the detection test tube 6 when clamping. Damage, a spring 44 is sleeved on the outer side of the rod body of the push-pull rod 41 located between the arc-shaped clamping plate 43 and the vertical part of the L-shaped fixing plate 3, and the two ends of the spring 44 respectively conflict with the arc-shaped clamping plate 43 and the vertical part of the L-shaped fixing plate 3; in this design, through the clamping assemblies 4 symmetrically arranged on both sides of the filter cartridge 1, when the filter cartridge 1 is extended into the detection test tube 6, the hand-held plates 42 in the clamping assemblies 4 on both sides are pulled outward, and then the arc-shaped clamping plate 43 is driven by the push-pull rod 41 to overcome the elastic force of the spring 44 and move toward the outside of the detection test tube 6. When the horizontal part of the L-shaped fixing plate 3 is placed at the mouth of the detection test tube 6, the hand-held plate 42 is released, and then the elastic restoring force of the spring 44 pushes the arc-shaped clamping plate 43 to be clamped and fixed to the outer wall of the detection test tube 6, so that the filter cartridge 1 can be stably set in the detection test tube 6.
[0020] In one embodiment, referring to Figure 2 and Figure 3 The limit assembly 5 includes an adjusting threaded rod 51, a rubber contact 52 and a fixing nut 53. A fixing nut 53 is fixedly provided at a side of each L-shaped fixing plate 3 at a position below the clamping assembly 4. The fixing nut 53 is internally threaded with an adjusting threaded rod 51. A rotating head 54 is fixedly provided at one end of the adjusting threaded rod 51 located at the outer side of the vertical portion of the L-shaped fixing plate 3. A rubber contact 52 is fixedly provided at one end of the adjusting threaded rod 51 located at the inner side of the vertical portion of the L-shaped fixing plate 3. The rubber contact 52 is arranged toward the filter cartridge 1. In this design, by the limit assembly 5 symmetrically arranged on both sides of the filter cartridge 1, when the clamping assembly 4 is clamped, the rotating heads 54 at the ends of the adjusting threaded rod 51 in the limit assembly 5 on both sides of the filter cartridge 1 are simultaneously twisted, and then the adjusting threaded rod 51 is screwed in, thereby driving the rubber contact 52 on the other end thereof to move and contact the outer wall of the detection test tube 6, so that the body of the filter cartridge 1 is located at the central axis position inside the detection test tube 6, thereby avoiding the outer wall of the filter cartridge 1 from contacting the inner wall of the detection test tube 6.
[0021] In one embodiment, in combination Figure 2A shielding flare 7 is extended outward from the edge of the top opening of the filter cartridge 1, and the inner diameter of the shielding flare 7 is larger than the inner diameter of the detection test tube 6. As a result, when the filtrate is dripped from the top opening of the filter cartridge 1, the shielding flare 7 can prevent the filtrate from dripping directly into the detection test tube 6 from the gap between the filter cartridge 1 and the detection test tube 6, thereby affecting the quality of the detection sample.
[0022] Working principle: During the filtering operation, the through-hole at the bottom end of the filter cartridge 1 is inserted into the test tube 6, and the hand-held plates 42 in the clamping assemblies 4 on both sides are pulled outward, and then the push-pull rod 41 drives the arc-shaped clamping plate 43 to overcome the elastic force of the spring 44 and move toward the outside of the test tube 6. When the horizontal part of the L-shaped fixing plate 3 is placed at the mouth of the test tube 6, the hand-held plate 42 is released, and then the elastic restoring force of the spring 44 pushes the arc-shaped clamping plate 43 to clamp and fix it on the outer wall of the test tube 6, so that the filter cartridge 1 can be stably set in the test tube 6. After the clamping assembly 4 is clamped, At the same time, turn the rotating heads 54 at the ends of the adjusting threaded rods 51 in the limit assemblies 5 on both sides of the filter cartridge 1, and then screw the adjusting threaded rods 51 inward to drive the rubber contacts 52 on the other end thereof to move and contact the outer wall of the detection test tube 6, so that the body of the filter cartridge 1 is located at the center axis position inside the detection test tube 6, thereby avoiding contact between the outer wall of the filter cartridge 1 and the inner wall of the detection test tube 6, and then drip the filtrate from the opening at the top of the filter cartridge 1. When the filtrate flows downward in the filter cartridge 1, it can flow and filter along the circumferential inclined surface of the conical filter screen 2 and drip out from the through-hole at the bottom of the filter cartridge 1 to the bottom of the detection test tube 6.
[0023] The above embodiments are only used to illustrate the technical solutions of the embodiments of the present application, rather than to limit them. Although the embodiments of the present application are described in detail with reference to the above embodiments, it should be understood by those skilled in the art that the technical solutions described in the above embodiments can still be modified, or some of the technical features can be replaced by equivalents, without departing from the spirit and scope defined by the claims of the present application.
Claims
1. A filtering device for sample pretreatment, comprising a filter cartridge (1), a filter screen (2), an L-shaped fixing plate (3), a clamping assembly (4) and a limiting assembly (5), characterized in that: The bottom of the filter cartridge (1) is a through structure. A filter screen (2) is fixedly provided on the inner side wall of the filter cartridge (1) near the bottom. L-shaped fixing plates (3) are symmetrically provided on both sides of the top of the filter cartridge (1). The horizontal portion of the L-shaped fixing plates (3) is placed at the position of the pipe mouth of the detection test tube (6). The vertical portion of the L-shaped fixing plates (3) is parallel to the axial direction of the pipe body of the detection test tube (6). A clamping assembly (4) is slidably provided on the vertical portion of each of the L-shaped fixing plates (3) toward the filter cartridge (1). The clamping assemblies (4) arranged on both sides of the filter cartridge (1) are symmetrically arranged. The clamping assemblies (4) are used to clamp and fix the detection test tube (6). A limit assembly (5) is threadedly connected at a position below the clamping assembly (4) on each of the L-shaped fixing plates (3). The limit assemblies (5) arranged on both sides of the filter cartridge (1) are symmetrically arranged. The ends of the limit assemblies (5) abut against the outer side walls of the detection test tube (6).
2. A filtering device for pre-treatment of detection samples according to claim 1, characterized in that: The filter screen (2) is of a conical structure, and the axis of the filter screen (2) coincides with the axis of the filter cartridge (1), and the circular end of the filter screen (2) is fixedly arranged on the inner wall of the filter cartridge (1), and the tip of the filter screen (2) extends toward the bottom through-hole of the filter cartridge (1).
3. A filtering device for pre-treatment of detection samples according to claim 1, characterized in that: The clamping assembly (4) comprises a push-pull rod (41), a hand-held plate (42), an arc-shaped clamping plate (43) and a spring (44); the push-pull rod (41) is vertically slidably arranged on the vertical portion of the L-shaped fixing plate (3); the hand-held plate (42) is fixedly arranged at one end of the push-pull rod (41) located outside the vertical portion of the L-shaped fixing plate (3); the arc-shaped clamping plate (43) is fixedly arranged at one end of the push-pull rod (41) located inside the vertical portion of the L-shaped fixing plate (3); the inner arc surface of the arc-shaped clamping plate (43) is arranged toward the filter cartridge (1); the spring (44) is sleeved on the outer side of the rod body of the push-pull rod (41) located between the arc-shaped clamping plate (43) and the vertical portion of the L-shaped fixing plate (3); and the two ends of the spring (44) are respectively in contact with the arc-shaped clamping plate (43) and the vertical portion of the L-shaped fixing plate (3).
4. A filtering device for pre-treatment of detection samples according to claim 3, characterized in that: A rubber pad (45) is fixedly provided on the inner arc surface of the arc-shaped clamping plate (43).
5. The filtering device for pre-treatment of detection samples according to claim 3, characterized in that: Two push-pull rods (41) are arranged on the vertical portion of each L-shaped fixing plate (3) along its vertical direction.
6. The filtering device for pre-treatment of detection samples according to claim 1, characterized in that: The limiting assembly (5) comprises an adjusting threaded rod (51), a rubber contact (52) and a fixing nut (53); a fixing nut (53) is fixedly provided on the side of each L-shaped fixing plate (3) at a position below the clamping assembly (4); the fixing nut (53) is internally threadedly connected to the adjusting threaded rod (51); a rotating head (54) is fixedly provided at one end of the adjusting threaded rod (51) located on the outside of the vertical portion of the L-shaped fixing plate (3); a rubber contact (52) is fixedly provided at one end of the adjusting threaded rod (51) located on the inside of the vertical portion of the L-shaped fixing plate (3); and the rubber contact (52) is arranged toward the filter cartridge (1).
7. The filtering device for pre-treatment of detection samples according to claim 1, characterized in that: A shielding expansion opening (7) is provided at the top opening edge of the filter cartridge (1) and extends toward the outside thereof, and the inner diameter of the shielding expansion opening (7) is larger than the inner diameter of the detection test tube (6).